More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
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Our breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
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“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
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Additionally, each book published by IntechOpen contains original content and research findings.
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We are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
Simba Information has released its Open Access Book Publishing 2020 - 2024 report and has again identified IntechOpen as the world’s largest Open Access book publisher by title count.
\n\n
Simba Information is a leading provider for market intelligence and forecasts in the media and publishing industry. The report, published every year, provides an overview and financial outlook for the global professional e-book publishing market.
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IntechOpen, De Gruyter, and Frontiers are the largest OA book publishers by title count, with IntechOpen coming in at first place with 5,101 OA books published, a good 1,782 titles ahead of the nearest competitor.
\n\n
Since the first Open Access Book Publishing report published in 2016, IntechOpen has held the top stop each year.
\n\n\n\n
More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\n\n
Our breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\n\n
“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\n\n
Additionally, each book published by IntechOpen contains original content and research findings.
\n\n
We are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\n\n
\n\n
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1. Introduction
Recently, climate change is receiving much attention. Changes in the world’s climate have significant effect on water resources which affect the livelihood of people especially in hyper arid regions such as the Kingdom of Saudi Arabia (KSA). The KSA suffers an enduring water shortage problem, despite the fact that the agricultural activities consume up to 90% of the water amount in the Kingdom. Reference Evapotranspiration (ETo) is an agro-climatic property that involves temperature, humidity, solar radiation, and wind speed. Identifying changes in ETo can also help in future planning of agriculture-water projects and identify lower and higher ETo zones for proper planning and management of agricultural projects in arid regions.
1.1. Water resources and climate change
Water shortage is a swelling problem in the arid and semi-arid regions. Affected by its geographic location and its climate, the Kingdom of Saudi Arabia (KSA) suffers a severe water deficit. Even rain, which is the only renewable water source, comes in flash short duration storms of high intensity and most of it vanishes to evaporation. Thus, almost all agriculture of the kingdom is irrigated. Irrigation water, though, consumes 80 to 88 % of the total water consumption (Abu-Ghobar, 2000; Abderrahman, 2001). In addition to these water scarcity conditions, but it seems getting scary by the effects of climate change on the hydrological cycle and water supply. The quantity of irrigation water is determined initially by identifying the reference evapotranspiration (ETo). Several researches was conducted to detect climate changes, trends and variability in various parts of the world using some climate parameters such as air temperature, rainfall depth, ETo, and pan evapotranspiration ETp (Shwartz and Randall, 2003; Garbrecht, et al., 2004; Hegerl, et al., 2007; Fu, et al., 2009; Hakan, et al., 2010; Elnesr and Alazba, 2010; Elnesr et al., 2010a; and Elnesr et al. 2010b). The ETo parameter has a special importance because it combines changes in many other climate parameters including temperature, radiation, humidity, and wind speed. It has, however, direct influence on hydrologic water balance, irrigation and drainage canal design, reservoir operation, potentials for rain-fed agricultural production, and crop water requirements (Dinpashoh, 2006).
1.2. Climate change effect on evapotranspiration worldwide
Several studies conducted in North America have shown that some climate parameters are on the rise including ETo (Fehrman, 2007; Garbrecht et al., 2004; Szilagyi, 2001). Fehrman, 2007 found an increasing trend in ETo over the Mississippi area and that most of ETo increase can be attributed to the increase in July. He also found that the rate of ETo increase was 0.29 mm/years when his study period extended from 1940 to 1999 compared to 0.88 mm/year when the study period was limited to 1950 to 1999 records. The accelerated ET over North America is presumed to be due to a rise in temperature over the past century (Myeni et al. 1997, Milly and Dunne 2001). In the contrary ETo and pan evaporation has shown to decrease in China (Thomas, 2000, Liu et al., 2004) and at a rate of 1.19 mm/year (Song et al., 2010) despite the rise in maximum daily temperature. In the Tibetan Plateau ETo decreases as well at a rate of 1.31 mm/year or 2.0% of the annual total evapotranspiration (Shenbin et al., 2006). The decrease in ETo has been attributed to the decrease in wind speed and net radiation. In another study Gao et al., (2007) found that the actual evapotranspiration had a decreasing trend in most of the eastern part of china and there was an increasing trend in the western and the northern parts of northeast China and that the change in precipitation played a key role for the change of estimated actual evapotranspiration. Similar negative trends in pan evaporation were found in 24 out of 27 observation stations in a 19-year study in Thailand (Tebakari et al., 2005). In India, a significant decreasing trend was found in ETo all over the Indian plateau during the past 40 years, which was mainly caused by a significant increase in the relative humidity and a consistent significant decrease in the wind speed throughout the country (Bandyopadhyay et al., 2009). In Australia, Roderick and Farquhar (2004) found a decreasing trend in pan evaporation and conclude that Australia is becoming less arid. However, there is enough evidence now that a decrease in pan evaporation is an indicator to an increase in actual evaporation. This is what known now as the evaporation paradox (Hobbins et al., 2004).
Some researchers developed a hypothetical scenario to study the effect of possible increase on temperature over the ETo and subsequently on water supply. A study conducted by Abderrahman et al. (1991) concluded that in the KSA, a 1℃ increase in temperature would increase ETo from 1 to 4.5%. In another study, that includes selected cities in KSA, United Arab Emirates and Kuwait, Abderahman and Al-Harazin (2003) concluded that an increase in temperature by 1℃ would increase ETo over these area by a maximum of 20%. In general, studies involving ETo calculation seemed to be more limited worldwide compared to other climate parameters. In the other hand, regarding other climatic parameters, Hakan et al. (2010) reported an increasing trend in temperature and ETo in most of stations they analyzed in Turkey using Mann-Kendall analysis. Cohen and Stanhill, (1996) studied rainfall changes in the Jordan Valley/Jordan and found a tangible but insignificant decrease at a rate of -0.47 and -0.16 mm/year for two different stations. Similar conclusions were observed by Al-Ansari et al (1999) who observed a general decrease in rainfall intensity. Smadi (2006), and Smadi and Zghoul (2006) found a prompt shift in rainfall and temperature in Jordan. ElNesr et al (2010b) concluded that the Saudi Arabia and the Arabian Peninsula are suffering from a considerable warming trend form year 1980 to 2008. Still, Elnesr et al. (2010a) concluded that the percentage land area with annual ETo>4000 mm increased from about 20% to 40% in the period they studied. On the other hand, lower ETo values, less than 3600 mm, contracted from about 30% to 12%.
1.3. Objective of the study
This study aims to trace the ETo values over time throughout all the area of the Saudi Arabia, then to quantify the future of water demand according to the ETo trends
2. Material and methods
2.1. Geography of the Saudi Arabia
Saudi Arabia is the largest country of the Arabian Peninsula; it occupies about 80% of its area (Wynbrandt, 2004). The country lies between latitudes 16°21\'58\'\'N, and 32°9\'57\'\'N, and longitudes 34°33\'48\'\'E and 55°41\'29\'\'E, as illustrated in Fig. 1. Saudi Arabia has a desert dry climate with high temperatures in most of the country. However, the country falls in the tropical and subtropical desert region. Winds reaching the country are generally dry, and almost all the area is arid. Because of the aridity and the relatively cloudless skies, there are great extremes in temperature, but there are also wide variations between the seasons and regions (AQUASTAT, 2008).
2.2. Evapotranspiration calculation
Evapotranspiration was calculated using Food and Agricultural Organization (FAO) Penman- Monteith (PM) procedure, FAO 56 method, presented by Allen et al. (1998). In this method, ETo is expressed as follows:
where ETo is the daily reference evapotranspiration [mm day-1], Rn is the net radiation at the crop surface [MJ m-2 day-1], G is the soil heat flux density [MJ m-2 day-1], Ta is the mean daily air temperature at 2 m height [°C], U2 is the wind speed at 2 m height [m s-1], es: saturation vapor pressure [kPa], ea: actual vapor pressure [kPa], ∆ is the slope of vapor pressure curve [kPa °C-1], and γ is the psychometric constant [kPa °C-1].
Figure 1.
Geographic map of Saudi Arabia, showing 13 districts and 29 meteorological stations. Base map Src: NIMA (2003). Districts Src: MOMRA (2007), Topography Src: Albakry et.al. (2010)
The measured meteorological data available were Ta, Relative humidity (RH) and U2 whereas soil heat flux (G) was taken equal to zero, (Allen et al, 2005). The slope of the saturation vapor pressure curve (Δ) is computed by the following equation as in Murray (1967):
Δ=4098×eo[Ta](Ta+237.3)2E2
whereeo[Ta] is calculated according to (Tetens, 1930):
eo[T]=0.611exp(17.27TT+237.3)E3
The net radiation Rn was estimated as the difference between the net short wave incoming radiation Rns and the net long wave outgoing radiation Rnl. The calculation of Rns, and Rnl, followed the procedures outlined in Allen et al. (1998) and Doorenbos and Pruitt (1977). All radiation was computed in daily energy flux units (MJ m−2 day−1). Allen et al (1998) reported a validated formula to calculate the incoming solar radiation Rs from air temperature difference:
Rs=cRaTx−TnE4
where Ra: extraterrestrial radiation [MJ m-2 d-1], c: an adjustment coefficient =0.19 for coastal stations and 0.16 for inland stations; Tn: minimum dry bulb air temperature [°C], Tx: maximum dry bulb air temperature [°C]. The psychometric constant γ is evaluated as:
γ=0.00163PλE5
where P: atmospheric pressure [kPa], λ: latent heat flux [ MJ kg-1]. The atmospheric pressure is expressed as in Burman et al. (1987)
P=101.3(293−0.0065z293)5.26E6
where z: altitude [m]. The latent heat λ depends on the average temperature, Eqn(7), while it can be taken as an approximate value of 2.45 as reported by Harrison (1963) for Ta=20 ℃. In the current study, we chose to calculate the latent heat using Eqn(7).
λ=2.5−0.00236TaE7
The saturation vapour pressure, es, and actual vapour pressure, ea, are calculated according to Allen et al (2005) as:
es=0.5(eo[Tn]+eo[Tx])E8
ea=0.005(RHxeo[Tn]+RHneo[Tx])E9
where RHx, RHn: maximum and minimum relative humidity [%] respectively.
The average daily ETo in a specific month was calculated by taking the arithmetic average of the daily values in that month. The summation of all ETo daily values in a year for a station will give the total annual ETo for that station.
2.3. Climatic data source and description
Basic climatic data were taken from the Presidency of Meteorology and Environment in KSA, the official climate agency in the country. The data set is the most accurate one in KSA and used by all other governmental and academic agencies for climate research and prediction. Weather stations are equipped with up-to-date monitoring devices and subjected to regular inspection and replacement for defected devices (personal communication with the Presidency of Meteorology and Environment). Data represents 29 meteorological stations as shown in Fig. 1. These stations represent all the 13 districts of the KSA. The data covers 29 years of daily meteorological records for 20 stations, 24 years for 6 stations, and 3 stations with less than 20 years as shown in Table 1. All of the data ends in 2008 and started at 1980 and 1985 for the 29 and 24 years logging.
District
Station
Station coordinates
RecrodedYears**
ID
Name
Latitude
Longitude
Altitude
Deg. N.
Deg. East
m
NorthernBorders
1
Turaif
31.41
38.4
818
29
2
Arar
31.00
41.00
600
29
5
Rafha
29.38
43.29
447
29
AlJouf
3
Guraiat*
31.50
37.50
560
4
4
Al Jouf
29.47
40.06
671
29
Tabuk
7
Tabuk
28.22
36.38
776
29
10
Wejh
26.12
36.28
21
29
Ha\'il
9
Hail
27.26
41.41
1013
29
AlQaseem
11
Gassim
26.18
43.46
650
29
EasternRegion
6
Qaisumah
28.32
46.13
358
29
8
Hafr Al-Batin
28.20
46.07
360
19
12
Dhahran
26.16
50.10
17
29
13
Dammam*
26.42
50.12
1
9
14
Ahsa
25.30
49.48
179
24
Riyadh
16
Riyadh North
24.42
46.44
611
24
17
Riyadh Middle
24.63
46.77
624
29
23
W-Dawasir
20.50
45.16
652
24
Madina
15
Madina
24.33
39.42
636
29
18
Yenbo
24.09
38.04
6
29
Makkah
19
Jeddah
21.30
39.12
17
29
20
Makkah
21.40
39.85
213
24
21
Taif
21.29
40.33
1454
29
Baha
22
Baha
20.30
41.63
1652
24
Aseer
24
Bisha
19.59
42.37
1163
29
25
Abha
18.14
42.39
2093
29
26
Khamis Mushait
18.18
42.48
2057
29
Nagran
27
Nejran
17.37
44.26
1210
29
28
Sharurrah
17.47
47.11
725
24
Gizan
29
Gizan
16.54
42.35
3
29
Table 1.
Geographical information of the meteorological stations included in this study.
2.4. Data grouping and contouring
After correction the data sets, daily ETo values were calculated for each station, then aggregated to annual and monthly values. Annual ETo value (mm/year) for each station was calculated by summation of the daily ETo for the entire year. On the other hand, the monthly average ETo value was calculated by taking the average of the daily ETo values during each month.
Evapotranspiration data were graphically represented by contour maps irrespective of stations altitude. Analysis of ET variations with stations’ altitude for each of the 30 years under study revealed no trends. Other researchers have also found no correlation between ET and station altitude in China (Thomas, 2000). Contour maps present clearly zones of common ET values as well as clarify vividly ET differences between zones and viability a long months or years. This approach has also been adopted by other researchers to study ET variability in China (Thomas, 2000; Shenbin et al., 2006).
Data was arranged in three columns format namely, longitude, latitude, and ETo. Each set of data was gridded separately using the ordinary point-Kriging method which estimates the values of the points at the grid nodes (Abramowitz and Stegun, 1972, and Isaaks and Srivastava, 1989). This procedure is used by SURFER™ Software which has been used in our calculations. The resulted grid was blanked outside the political borders of the KSA. The political borders’ information of the KSA was grabbed from electronic map of NIMA (2003). The electronic map was digitized and converted to DMS geographic coordinate system. The blanked grid was plotted as a contour map using Surfer™ 8.0 software (Surfer, 2002). Sample plots for the average daily ETo during a month, June in this case and the ETo, in a year, 1991 in this case, is shown in Figure (#2a, b), respectively, where darker areas represent smaller magnitudes of ETo.
Figure 2.
Sample contour map of daily and annual evapotranspiration in the KSA. (a): average daily evapotranspiration for the month of June over 30 years period (mm/d). (b): annual evapotranspiration of the year 1991 (mm/y)
All of the data are daily values, the obtained climatic data records were carefully inspected for missing and erroneous reading. Very few errors were found, (median value of 00.45%). Errors were classified into four categories: Errors because of mistaken extreme values such as a relative humidity exceeds 100% or below 0%. Illogical errors such as the recorded maximum daily temperature (Tx) was less than the minimum daily temperature (Tn) in the same day, or if Tx = Tn. Missing values; i.e. Tx is present but Tn is missing. Recording an error-indication number (like 999 or 777) if the sensor is not functioning. On analyzing the data record, any value contains one or more errors was considered missing record unless the missing record could be predicted with minimal error, i.e. if the average temperature (Ta) is missing while Tx and Tn are logged with no errors; in this case Ta = (Tx + Tn)/2. However, the amount of missing data in the recorded period could be considered negligible in most of the stations, where the average amount of missing data is 0.78%.
2.5. Non-parametric trend analysis methods
2.5.1. Mann-kendall test
The Mann-Kendall test is a non-parametric test used for identifying trends in time series data. The test compares the relative magnitudes of sample data rather than the data values themselves Both Kendall tau coefficient (τ) and Mann-Kendall coefficient (s) are nonparametric statistics used to find rank correlation. Kendall (τ) is a ratio between the actual rating score of correlation, to the maximum possible score. To obtain the rating score for a time series, the dataset is sorted in ascending order according to time, and then the following formula is applied:
s=∑j=1j=n−1∑i=j+1i=nSign(xi−xj)E10
where s: the rating score (also called the Mann-Kendall sum); x: the data value; i and j: counters; n: number of data values in the series; Sign is a function having values of +1, 0, or -1 if (xi-xj) is positive, zero, or negative, respectively. According this formula, the maximum value of s is:
smax=12n(n−1)E11
Hence, the Kendall (τ) is calculated as:
τ=ssmaxE12
A positive value of s or τ is an indicator of an increasing trend, and a negative value indicates a decreasing trend. However, it is necessary to compute the probability associated with s or τ and the sample size, n, to quantify the significance of the trend statistically. Kendall and Gibbons (1990) introduced a normal-approximation test that could be applied on datasets of more than ten values with s variance (σ2):
σ2=118n(n−1)(2n+5)−CFRE13
CFR=118∑k=1gmk(mk−1)(2mk+5)E14
where CFR: repetition correction factor, to fix the effect of tied groups of data (when some of the data values appear more than one time in the dataset, this group of values are called a tied group); g: number of tied groups; k: a counter; m: number of data values in each tied group. Then normal distribution parameter (called the Mann-Kendall statistic, Z) is calculated as follows:
Z={1σ(s−1)→s>00→s=01σ(s+1)→s<0E15
The last step is to find the minimum probability level at which the parameter Z is significant, this could be found using two-tailed t statistical Tables or as mentioned by Abramowitz and Stegun (1972):
αmin=(b0e−0.5Z2)∑q=1q=5bq⋅(1+b6ABS(Z))−qE16
where αmin: Minimum level of significance; q: counter; bx: constants: b0= 0.3989, b1= 0.3194, b2= -0.3566, b3= 1.7814, b4= -1.8213, b5= 1.3303, b6= 0.2316, ABS(Z): the absolute value of Z. Kendall tau is considered significant when alpha min is less than a specified alpha value, i.e 0.05.
2.5.2. Sen-slope estimator test
Sen’s statistic is the median slope of each point-pair slope in a dataset (Sen, 1968). To perform the complete Sen’s test, several rules and conditions should be satisfied; the time series should be equally spaced, i.e. the interval between data points should be equal. However, Sen’s method considers missing data. The data should be sorted ascending according to time, and then apply the following formula to calculate Sen’s slope estimator (Q) as the median of Sen’s matrix members.
Q=Median{[[xi−xji−j]j=1j=n−1]i=j+1i=n}E17
Its sign reflect the trend’s direction, while its value reflects how steep the trend is. To determine whether the median slope is statistically different than zero, the variance is calculated using Eqn. (4), to obtain the confidence interval of Q at a specific probability level, e.g 95%. The area (Z) under two-tailed normal distribution curve is calculated at the level (1-α/2), where α=1-confidence level. For example, for a confidence level of 95%, Z should be evaluated at 0.975, hence Z= 1.96. Next, the parameter Cα is calculated as follows:
Cα=Z1−α/2σ2E18
The upper and lower confidence boundaries for Q are then calculated as follows:
Mu=int(0.5(nq−Cα))Ml=int(0.5(nq+Cα))+1E19
where int() represents the integer value; Mu and Ml are the upper and lower boundaries for Q at 1-α probability level; nq is the number of Sen’s matrix members calculated from Equation (17), equal to nq=n(n-1). The median slope is then defined as statistically different from zero for the selected confidence interval if the zero does not lie between the upper and lower confidence limits.
Figure 3.
The monthly average ETo variations during the year in KSA, vertical bars indicate standard deviation values.
3. Results and discussion
The daily ETo data for each of the studied stations were calculated for the study period. Then we summarize the data on monthly basis to find the average, maximum and minimum values per month for the whole country, Figure 3. The lowest values of ETo occurred in the winter season, December and January whereas the highest values occurred during summer months; June, July and August. The results showed high variation in ETo from about 5 mm/day to 15 mm/day in July whereas the absolute minimum and maximum ETo showed even higher variation and ranged from 3.9 mm/day in January to as high as 18.5 mm/day in July. However, Figure 2 indicates the high variability of climate conditions over Saudi Arabia, which highlights the major challenge for agricultural development and water resources planning in the country.
Figure 4.
Relationship between the average annual ETo (mm/d) as related to station altitudes, latitudes, and longitudes.
The variation of ETo over the area of Saudi Arabia is large, as it should be due to its large surface area and large differences in altitudes. Therefore, the average ETo values in the study period for the studied stations were plotted with their altitude, latitude and longitudes and the results are shown in Figure 4. As expected ETo decreased with station altitude as weather stations varied from sea level, Yenbo’ and Jeddah stations to as high as 2300 m above sea level in Nejran. Similar relationships were found with temperature and stations altitudes in the study of ElNesr et al., (2010b), which may be a significant reason for rising ETo values. However, large variabilities were also observed among stations at the sea level suggesting that other factors may have compound effect on ETo in Saudi Arabia such as the geographic location (latitude and longitude). While latitudes seemed to have no effect on the variability of ETo, longitudes have tangible effect on it,. ETo increased steadily with stations longitudes as we travel toward the east. The concentration of oil industries and refineries in the eastern parts of KSA may have affected air temperature, while the nearness to the Arabian Gulf increased the relative humidity, thus leads to raising ETo in the eastern parts of KSA relative to the other parts.
The changes in ETo with time was examined by calculating the average daily evapotranspiration (mm/d) over the whole study period of each station, and plotting time series ETo for the study period, Figure 5. The Figure shows clearly a positive trend of ETowith time during the study period. The ETo has increased from about 9.6 in 1980 to about 10.4 mm/day in 2008 at a rate of 0.02 mm/day. Regression analysis between ETo and time has confirmed the positive trend and showed that the slope of the line was 0.020 with R2 = 0.50. However, this relationship was not statistically significant at 95% probability level. Longer period of data analysis is needed to confirm this result. Nevertheless, the present analysis indicates clearly that climate variability is indeed affecting the country and the evapotranspiration demand is increasing with time.
Due to some restrictions in Man-Kendall and Sen’s methods, two stations out of the 29 stations were omitted from calculations due to the small number of years they had (less than 10 years); those were stations #3 (Guraiat) and # 13 (Dammam). Mann-Kendall and Sen Slope statistics were performed on the rest 27 stations on monthly basis to confirm trends direction and test its significance. Two parameters were calculated namely Kendall τ and Sen Slope Q and their confidence limits at 95% and 99% probability level as described in Materials and Methods. A group of selected results is shown in Figure 6 where the parameters of Mann-Kendall and Sen Slope and their significant tests are presented. The Figure represents ET trends in January for four stations, Tabuk, Sharurrah Yenbo, and Hail, showing possible combinations of Mann-Kendall (MK) and Sen Statistic, in addition to their significance under increasing or decreasing ETo conditions. That is, Figure 6a showing a downtrend with MK and Sen significant at 95% and 99%. Figure 6b showing a downtrend with only MK is significant at 95%. Figure 6c showing an uptrend with all statistics was significant. Figure 6d showing an uptrend with only MK is significant at 95%.
Figure 5.
Temporal change of the average, maximum, and minimum ETo through the study period.
The two tests gave similar results in all of these cases but Sen Slope test were found to be more conservative. A positive sign in τ or Q indicates an increasing trend, Figure 6 C and D while a negative value indicates a decreasing trend, Figure 6 A and B. The significance of τ was tested by comparing the calculated αmin with α = 0.05 or 0.01 for 95% and 99% confidence level, respectively; αmin < 0.05 or 0.01. The corresponding significant test for Q was carried out by calculating its confidence intervals at 95% and 99% indicated by (Qmin95%, Qmax95%) and (Qmin99%, Qmax99%), respectively. If the two limits have similar sign, then the calculated Sen Slope Q value is confirmed not to be zero and therefore the slope is significantly different from zero, indicating a positive or negative trend for +Q or -Q, respectively.
\n\t\t\t\t
Figure 6.
Analysis of monthly average ETo trends using Mann-Kendall and Sen slope estimator and their significant tests parameters: A: Tabuk area, B: Sharurrah area, C: Yenbo area, and D: Hail area.
Figure 6A-D represents four possible cases of Q and τ and their significance. In Tabuk and Sharurrah, both Q (-0.020, -0.028) and τ (-0.365, -0.281), respectively, were negative indicating a decreasing trend for ETo. However, this decrease is significant for Tabuk and not significant for Sharurrah at 99% probability level as confirmed by both statistical methods. In the case of Tabuk, the value of calculated αmin = 0.0029 is less than 0.01 indicating a significant trend according to Mann-Kendall test; for Sen slope test the 99% minimum and maximum values of the confidence interval are both negative indicating that the slope Q is not zero and therefore a negative trend is confirmed. However, for Sharurrah stations αmin = 0.03 is less than 0.05 but larger than 0.01 indicating that the positive trend is significant at 95% but not at 99% level according to Mann-kendall test. A slightly different result was found in Sharurrah with Sen slope test. The upper limits for the Q confidence interval at 95% and 99% were both larger than zero, Qmax95%=0.004, Qmax99%=0.015 indicating that the median slope of the ETo series, Q, can actually be zero and therefore the negative trend is not significant at both levels. This results show that Sen Slope test can be more conservative than Mann-Kendall test.
Both Yenbo and Hail have increasing ETo trends, as shown in Figure 6C and D, because τ and Q values were both positive (Q= 0.022, τ= 0.515 for Yenbo; while Q= 0.01 and τ = 0.24 for Hail). Both tests showed that ETo in Yenbo is increasing significantly at 95% as well as at 99% level since τ and Q were positive and αmin = 0.000 < 0.05 and Qmin95%> 0.0. However, in Hail this uptrend was significant according to Mann-Kendall (αmin = 0.000 < 0.05) at 95% level but not significant according to Sen Slope since Qmin95%=−0.001 indicating the possibility of the slope being zero. These results indicated the validity of these two statistical methods to detect trends in a time series data.
The previous analyses shown in Figure 6 were carried out for the 27 stations out of 29 under study. Man-Kendall, and Sen’s methods’ can deal with data series with 10 or more data points. However, Gurrayat and Dammam have less than 10 years of data and they were excluded from trend analysis. Average ETo time series were analyzed for each month and the resultant Sen slope Q and Kendall τ are shown in Table 2 and Table 3, respectively followed by up or down arrows to indicate their significance. Up-arrows in light or dark black indicate significance up trend while similar light and dark black down arrows indicate a significant down trends at 95% and 99% probability level, respectively. Numbers without arrows are not statistically significant. The total number of stations with a decreasing or increasing trend in each month were calculated and shown at the bottom of the Table. whereas the number of months at which stations showed a decreasing or increasing trends were shown for each station at the right side of the Table. Numbers between brackets indicates the number of months or stations with the corresponding significant trend.
The tests were carried out for maximum, minimum and average monthly ETo but only the average ETo is shown in the Tables, because extreme ETo showed similar behavior to that of average ETo.
Table 2.
Monthly trends in the average ETo as estimated from Sen’s slope statistics (Q) for various meteorological Station. Numbers in parenthesis indicate number of significant values.
Table 3.
Monthly trends in the Average ETo as estimated from Mann-kendall statistics (τ) for various meteorological Station. Numbers in parenthesis indicate number of significant values.
Fourteen stations have a positive Q and τ, for at least 10 months in a year therefore an uptrend in ETo namely; Turaif, Arar, Al jouf, Hafr Al-Baten, Hail, Gassim, Dhahran, Riyadh (old), Yenbo, Taif, W-dawaser, Bisha, Abha, and khamis Mushait. Another six stations showed a negative or zero Q during the whole year, therefore a downtrend in EToincluding; Tabuk, Wejh, Makkah, Nejran, Sharurrah, and Gizan. The other seven stations showed a mix of increasing and decreasing trends during the year and those are Rafha, Qaisumah, Ahsa, Madina, Riyadh (new), Jeddah, and Baha.
However, the up or down trends or downtrends in ETo in the first mentioned group were not always significant as indicated by the upward arrows and summed in the last two columns of Tables 2 and 3. Only Yenbo had a confirmed significant trend at 95% level during the entire year. Other stations showed a significant up trends in ETo for several months during the year including Hail and Taif, 10 months; Turaif and Arar, 9 months; Al jouf and Khamis Mushait, 8 months. The other stations among the uptrend group had significant uptrend in 3 months to 7 months in a year as shown in Table 2.
The number of stations with a decreasing trend is far less than those with increasing ETo. Few stations showed a decreasing trend in ETo for 9 months or higher including, Qaisumah, Tabuk, Wejh, Ahsa, Makkah, Nijran and Sharurrah. However, only Ahsa station had a significant decreasing trend for 7 months followed by Qaisumah and Sharurrah, 4 months, and Tabuk with only 3 months of declining in ETo. The rest of stations, Wejh, Makkah, Nejran, Gizan, had a decreasing trend but this trend is not significant at 95% probability level.
The numbers of stations with increasing or decreasing trend are shown in the last two rows of Table 2 and 3. Figure 7, as well, illustrates the number of stations with significant/non-significant increasing/decreasing trend of ET throughout the studied areas. At least 15 stations or higher showed an increasing trend for the entire year except in January at which 14 stations showed a decreasing trend. March, April and June showed the highest number of stations with increasing ETo. However, the significant increase in ETo were confirmed for about 10 stations and for 9 months, February to October. During the months of October to January about 10 stations showed a decreasing trend but this decrease was significant for only 4 stations in September and October, one station in November and December and 3 stations in January.
Further inspection on the location of stations with increasing trends in ETo revealed that most of these stations are located in the northern part of the Arabian peninsula north to the latitude line of 22 degrees. However, some other stations were located southern of this line at the southern west corner of Saudi Arabia. It seemed that stations located along the longitudinal line of 45 degrees showed an increasing trend. Actually the wind direction over the Arabian peninsula seemed to follow this line from south west to north in rainy seasons and from north to south west in the dry seasons.
To have an aerial graph for the regions with a decreasing or increasing trend in ETo a contour map were plotted for each months and the results are shown in Figure 8. Certainly, regions with increasing trends are concentrated in the northern part of Saudi Arabia and extended to the south along the 45-degree longitudinal line. Significant and increasing regions, indicated by black and grey regions (P>95%) are prevail for most of the year except in January and July to some extent. In January most of SA areas have decreasing ETo as shown in Figure 8 and also in Table 3 where 14 stations have a decreasing trends; although this decrease is not significant at 95% level except for 3 stations. The southeastern parts seemed to have decreasing trends most of the years, but also this trend is not significant at 95% except in July and October as indicated by the darker dotted regions.
Figure 7.
Number of stations with significant/non-significant increasing/decreasing trends of ET in the studied areas.
Figure 8.
Contour plots showing the distribution ETo trends’ direction and confidence level over the area of KSA.
4. Conclusion
Water scarcity problem can be solved by proper management of water usage. Most of the depleted water in KSA is consumed through agriculture. Identifying the ETo trend and knowing the zones having the least ETo values can help in determining the future plans of agricultural and water extensions. Historical analysis of daily ETo in Saudi Arabia was carried out using Penman Monteith equation (FAO-56) for 29 meteorological stations distributed all over Saudi Arabia for the period 1980 to 2008. The long time average daily ETo varied from about 5 mm/d in Jan to 15 mm/day in July which is one of the hottest months in the country. ETo time series analysis using Mann-Kendall and Sen slope statistics revealed that ETo has been increasing steadily during the study period. The average minimum and maximum daily ETo increased steadily and ETo average increased from about 9.6 to about 10.4 mm/day in 2008. Trend analysis revealed that about 14 of the weather stations showed a significant increasing trend in ETo during the year for more than 7 months. Only 4 stations showed decreasing trends in three months, September, October and January.
Increasing ETo trends prevail in the northern and south-west areas along the longitudinal line of 45 degrees while decreasing trends prevail in the north western spot along the red sea and south eastern parts along the Arabian Gulf. This demonstrates that ETo fluctuation is increasing with time that can be considered a significant sign for climate variability in the Arabian peninsula. This increase in ETo seemed to be mainly affected by the global warming or the increase in temperature in the Arabian peninsula which was confirmed by several studies mentioned in this paper. Analyses of longer historic data are needed to confirm these findings. This demonstrates that ETo fluctuation is increasing with time that can be considered a significant sign for climate change. Though, the findings of this research suggest the needs to consider ETo changes in the planning for agricultural and water resources projects. Thus to rank the areas with fixed and decreasing ETo trend as highly recommended zones for future agricultural projects, and to do the opposite with the increasing ETo trends’ zones. Finally, if the low ranked zones are essential due to other circumstances, then the water management policy should consider the increment rate in ETo and its effect on water consumption.
Acknowledgement
The authors wish to express their deep thanks and gratitude to “Shaikh Mohammad Bin Husain Alamoudi” for his kind financial support to the King Saud University, through the research chair “Alamoudi Chair for Water Researches” (AWC), where this paper is part of the AWC chair activities. Thanks should also be expressed to the Presidency of Meteorology and Environment in Riyadh, KSA, who kindly support this research my meteorological data.
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1. Introduction
Spider mites (family Tetranychidae) contains many species that are important pests of agricultural crops. According to Migeon & Dorkeld [1], who provided a database for spider mites of the world, 1300 species had been described until now. Practically all the major food crops and many ornamental plants are subject to attack [2]. Tetranychid mites feed by penetrating the plant tissue with sharp cheliceral stylets and removal of the cell contents. The chloroplasts disappear and the small amount of remaining cellular material coagulates to form an amber mass. The amount of chlorophyll in the leaves may be decreased as much as 60 percent. The mite feeding also causes inhibition of photosynthesis. Small chlorotic spots can be found at feeding sites as the mesophyll tissue collapses due to the destruction of 18–22 cells per minute. Additionally they produce silk webbing which covers the leaves. Continued feeding leads to irregular spots formed by the integration of primary suction spots; finally the leaves turn yellow, gray or bronze. In the case of sever infestation the death of plants occur [3].
A rapid rate of mite development and high reproductive abilities allow spider mites to reach harmful population levels very quickly when the conditions for growth are permissive. A great number of experimental work has been directed toward the control of these mites since they have become resistant to a number of pesticides and their control has become very difficult. Moreover, chemical suppression of mite populations leads to residues on crops, environmental contaminations and toxicity to humans and non-target organisms. For these reasons, research has increasingly been performed to identify alternative methods to chemical control [4].
2. Tetranychidae control Methods
2.1 Chemical control
Prior to world war II, spider mites were minor pests of agricultural crops. This changed rapidly after war, with the extensive use of chemical pesticides, such as DDT [5]. The chemical acaricides used to control Tetranychidae are characterized by a large variety of chemical structures and mode of actions which were reviewed by Attia et al. [6], Knowles [7] and Dekeyser [8]. A pesticide may have both direct and indirect effects on Tetranychidae. Some may kill immediately, while other pesticides take longer to kill. Others may affect mite performance by inhibiting movement and reducing searching ability or lowering oviposition rates. In addition some pesticides (such as carbaryl and DDT) have a stimulatory effect on spider mite reproduction when present in low concentrations. The stimulatory effect on mite reproduction is called hormoligosis. Hormoligosis is an ongoing problem, although it may not be recognized [9]. The chemical control of these mites has become increasingly difficult because of their short life cycle, abundant progeny and arrhenotokous reproduction system. The repeated use of pesticides can lead to the development of resistant population and also can disrupt the natural control of Tetranychidae. Because of its resistance to a large number of chemical compounds, the two-spotted spider mite, Tetranychus urticae Koch, is considered most resistant species nearly in all over the world [10].
2.2 Cultural control
Cultural control involve all agronomic practices that are intended to reduce pest population. Cultural practices include changing the time of planting and harvest to avoid or minimize pest damage. It is known that high humidity reduce the reproductive potential of Tetranychidae whose optimal environment is hot and dry air [11]. Proper management of temperature and humidity can be useful to reduce pests’ populations in greenhouses. Managing fertilizer applications is another important cultural practice. Large quantities of nitrogen or deficiency of potassium can increase the amount of soluble nitrogen available in the plant so that cause population increase of T. urticae [11]. In our previous work on the effect of fertilizer Fosfalim-k application on cucumber and its effect on population growth of T. urticae we showed that its application in the recommended dose had a controlling effect [12].
Another example of cultural control is dust management. Dust management is important for control of Tetranychidae, especially in climates that crop irrigation occurs. Whether the dust makes the foliage more suitable for spider mites or interferes with the spider mites predators’ performance is in controversy. The elimination of crop residues is another way that can destroy pests and prevent transferring to subsequent crops. Crop rotation and polycropping are other methods that can be used to manage pest population. It is not clear that polycropping is useful in phytophagous mites control but if natural enemies are retained in the crops it could be helpful [9]. In our previous work we showed that the intercropping of sunflower and soybean increased natural enemies compared with monocultures [13].
2.3 Host plant resistance
Host plant resistance along with cultural control, is a component of any pest management program. Resistance of plants to pests enables them to avoid or inhibit host selection, inhibit oviposition and feeding, reduce pest survival and development and tolerate or recover from injury of pests that would cause greater damage to other plants of the same species under similar environmental conditions [14, 15]. Three mechanisms of plant resistance to pests have been categorized by Horber [16]: antixenosis, antibiosis and tolerance. Antixenosis describe the inability of a plant to serve as a host to a pest. The basis of this resistance mechanism can be morphological (e.g. leaf hairs, surface waxes and tissue thickness) or chemical (e.g. repellents or antifeedants). Antibiosis is the mechanism that describe the negative effects of a resistant plant on the biology of a pest which has colonized on the plant (e.g. adverse effects on development, survival and reproduction). Both morphological and chemical characteristics of plants can induce antibiosis. Tolerance is the degree to which a plant can tolerate a pest population that under similar conditions would severely damage a susceptible plant [17]. Resistance against spider mites is known to occur in many crops, including melon, pepper, soybean, cotton, cucumber, bean, eggplant and tomatoes. Resistant cultivars can be discovered by comparing mite populations on different crop varieties grown under the same conditions with equivalent initial mite populations [9]. We discovered the antibiosis mechanism of resistance to T. urticae in pepper varieties (unpublished data).
2.4 Biological control
Biological control is the use of natural enemies to manage pests’ populations. Natural enemies are very important agents in reducing or regulating populations of pests and include parasitoids, predators and pathogens. A parasitoid is an organism that spends its larval stage in or on another organism, also known as a host. The larval parasitoid feeds only on the host as it develops, eventually killing the host. There are no report of mite’s parasitoids. Predators are free living organisms, each of which will consume a number of pests (prey) in their lifespan. More than 65 predators have been recorded for European red mite, Panonychus ulmi (Koch), alone. Among the more important of these biological agents are predatory mites and insects, but others include spiders and disease-producing pathogens [3]. Three major methods exist for the use of natural enemies: conservation, classical biological control and augmentation.
Conservation seeks to identify and rectify negative influences of human activities that suppress natural enemies and to enhance agricultural fields as habitats for natural enemies. In conservation, the assumption is that the species of natural enemies already exist locally and have potential to effectively control the pest if given an opportunity to do so [18]. Classical biological control involves importation, evaluation, release and permanent establishment of natural enemies in the environment from the area of origin of a foreign pest. It assume that natural enemies from the area of the pest’s origin will be more effective than natural enemies in the pest’s new environment [9]. Augmentation involves the mass rearing and release of natural enemies to control target pest. The natural enemies must be capable of being mass reared and must be released at an appropriate time and in sufficient number to be effective. Two approaches are taken in augmentation. Inoculation involves releasing small number of natural enemies early in crop cycle with the expectation that they will reproduce and their offspring will provide pest control for an extended period of time. Inundation involves releasing large number of natural enemies for immediate control of pest when insufficient reproduction of the released natural enemies is likely to occur [18].
We found predatory mites from families Phytoseiidae, Ameroseiidae, Parasitidae, Stigmaeidae, Anystidae and Bdellidae as natural enemies of Tetranychidae during our sampling from Northwestern Iran (2007–2008). Among predator insects, we found Stethorus gilvifrons Mulsant (Col.: Coccinellidae), Oenopia conglobata (Linnaeus) (Col.: Coccinellidae), Exochomus quadripustulatus (Linnaeus) (Col.: Coccinellidae), Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae), Scolothrips sp. (Thysanoptera: Thripidae) and Orius horvathi Reuter (Het.: Anthocoridae). Among the predatory mites that we found, here we describe, Phytoseius plumifer (Acari: Phytoseiidae), which we have been worked on it.
Predaceous mites of the family Phytoseiidae are important natural enemies of several phytophagous mites and other pests on various crops. Phytoseiid mites occur throughout the world. Several authors have considered Phytoseius plumifer among the most important predators of phytophagous mites infesting fruit trees [19]. Before using natural enemies in biological control programs, it is essential to evaluate their efficiency and therefore, knowledge of the behavioral attributes of P. plumifer is essential for understanding the efficiency of this predator in the biological control of two-spotted spider mite.
2.4.1 Prey stage preference, switching and mutual interference of Phytoseius plumifer
Prey stage preference may affect prey–predator population dynamics, if the prey stage affects the development and reproduction of the predator. Prey preference by biological control agents can affect their ability to effectively control target pests too [20]. Preference may vary with the relative abundance of two prey types, in which case if the predator or parasitoid eats or oviposits in disproportionately more of the more abundant type, it is said to display switching behavior. In other words, switching is a behavioral phenomenon whereby a predator alters its preference for the prey species or type as prey relative densities change [21]. Murdoch et al. [22] found that switching could result from several different mechanisms including when (1) the predator develops a search image for the prey type with the highest relative abundance, (2) capture success on a prey type increases with increase in its relative abundance and (3) when the predator’s habitat contains sub-habitats that are occupied by different prey types.
Aggregation of predators in space to prey patches causes the prey–predator interaction occur and searching efficiency to decrease with increasing predator density. Inverse density dependence in searching efficiency is known as predator interference or mutual interference. However, it was found that increasing the number of biological control agents released into an environment did not always increase the level of pest control [23]. This occurs when parasites/predators that are searching for a host/prey encounter each other, which can cause one or both to stop searching and possibly leave the area [24].
In our previous work we determined some aspects of the behavioral characteristics of P. plumifer on the two-spotted spider mite. We studied the preference of P. plumifer for different life stages of the two-spotted spider mite under choice and no-choice conditions. Switching of P. plumifer was tested with deutonymphs and larvae of the prey with different ratios too. Also, since the success of a predator in biological control programs is dependent on its behavior under the presence of other con-specific individuals, we investigated the mutual interference of P. plumifer in different densities of predator mites [25].
2.4.1.1 Materials and methods
2.4.1.1.1 No-choice experiment
In the feeding tests, we offered a total of 30 prey individuals of egg, larva, protonymph, deutonymph, male and female separately to a 24 h starved unmated female predator on soybean leaf arena and then allowed each predator to feed on the prey individuals for a total of 24 h. At the end of the experiment we estimated the number of prey individuals consumed per predator on each life stage of the prey.
2.4.1.1.2 Choice experiment
In this experiment we exposed total of 30 prey items i.e. equal number (5) of all stages of T. urticae (egg, larva, protonymph, deutonymph, male and female) to the predator females.
2.4.1.1.3 Switching
Switching of P. plumifer was tested with deutonymphs and larvae of the prey. Deutonymphs (D) and larvae (L) of T. urticae were presented in five different ratio treatments: 30 L:70D, 40 L:60D, 50 L:50D, 60 L:40D and 70 L:30D. The total prey number was 30. For evaluating the value of selectivity the following equation were used:
C=E1/E2E1
where E1 and E2 are the proportion of larvae and deutonymphs killed in 50 L:50D ratio, respectively. To find the expected ratio of killed larvae and deutonymphs in no-choice position the obtained data were analyzed by Murdoch [22] formula as follow:
Y=Cx/1–X+CxE2
where Cx is C× ratio of stage and X is the ratio of a prey stage on a leaf disc.
2.4.1.1.4 Mutual interference
In this experiment, 160 immature individuals (larvae and protonymphs) of T. urticae were placed on each leaf arena. In the next step, female predators at densities of 1, 2, 4, 8 and 16 per leaf arena were allowed to search the prey for 24 h. After this time period, the predators were removed from the arena and the number of eaten preys was counted. Finally, the per capita searching efficiency (a) of the predator at different densities was calculated according to the Nicholson [26] equation as follows:
a=1/PTlnNt/Nt−NaE3
where Nt is the total number of available prey (160), Na is the total number of eaten preys, P is the number of predators, and T is the duration of the experiment (set to 1.0 for one day).
The calculated searching efficiency (a) was fitted against predator density (both on a logarithmic scale). The points were fitted to a linear regression by the least square method, according to the inductive model given by Hassell and Varley [27] as follows:
a=QP−morloga=logQ–mlogPE4
where a is the searching efficiency of the predators, Q is the quest constant, and m includes only the component of interference due to behavioral interactions between predators [28].
2.4.1.2 Results
Our results indicated that in our no-choice preference experiments the predation preference of this predator on the different stages of T. urticae was as follow: eggs>protonymphs>larvae>males>deutonymphs>females of T. urticae. The preferred stage of two-spotted spider mite in choice preference experiments was protonymph. There was no tendency to the adult females of T. urticae in our results maybe because of their big size and the feeding rate was zero. Females of the predator killed more larvae than deutonymphs in switching experiments and they preferred larval stage compared to deutonymphs. There was positive switching behavior of predator for larval stage of prey at all ratios except 40%Larva: 60%Deutonymph (Figure 1) maybe because of their smaller size.
Figure 1.
Switching behavior of Phytoseius plumifer females to different ratios of larval stage and deutonymph of Tetranychus urticae.
The values of total predation rate of P. plumifer were significantly different at different densities of the predator and the highest and lowest values of this parameter were recorded at 16 and 1 density of this predator, respectively. Furthermore, the per capita predation rate decreased to 1/4 with increasing the predator density from 1 to 16 and consequently the per capita searching efficiency also decreased significantly. According to results of Murdoch et al. [22] mechanisms one and two appear likely for our predator and capture increases on a prey type with increasing in its relative abundance.
The linear relationship between the natural logarithm of the predator density and the natural logarithm of per capita searching efficiency in mutual interference analysis has been demonstrated a negative slope. The negative value of the interference coefficient in the mutual interference analysis showed an inverse relationship between the predator density and per capita searching efficiency and this fact revealed that the searching efficiency of P. plumifer significantly decreased with increasing predator density as a result of mutual interference. For most augmentative biological control agents, there is an optimal release rate that produces effective control of a pest species. Increasing the release rate above the optimal rate does not improve the control of pest species and is potentially economically detrimental [29]. In our study although with an increasing number of predators, greater numbers of preys have been consumed but, a doubling in the number of predator employed for T. urticae predation did not result in a doubling in the number of mite consumed, because of mutual interference. A significant decrease of the number of prey consumed per predator with an increased predator density suggests that interference among predators also increase at higher predator density. This is probably due to a closed experimental arena with limited predation time and high probability of mutual interference. However, under field conditions, factors such as large searching areas, the effects of other predator species, spatial complexity, and weather may affect the effectiveness of natural enemies [30].
2.4.2 Phytoseius plumifer performance feeding on corn pollen
Although phytoseiid mites have been mainly described as predators of mites and small insects, several species can feed and reproduce on pollen as well. The potential of phytoseiids to regulate phytophagous mites at low equilibrium densities has been more attended recently and studies have examined some of the characteristics that contribute to the survival of populations at low prey densities, such as feeding on pollens [31]. Pollen is utilized as an easy food source for phytoseiid mites rearing and also has been recognized as an important factor in the successful biological control of spider mites [32].
McMurtry and Croft [31] categorized the life style of phytoseiids based on feeding habitats and related biological and morphological traits. The life styles are: Type I, specialized predators of Tetranychus urticae; Type II, selective predators of tetranychids; Type III, generalist predators that may feed on pollen but perform better on prey; Type IV, specialized pollen feeders-generalist predators. Phytoseius species are categorized as Type III predators. Knowledge of the nutritional value of different plant pollens for P. plumifer could be important not only for mass rearing of the mite, but also for a better understanding of its population dynamics in the field.
In our previous work we described the effect of corn pollen on the life table parameters of P. plumifer at laboratory conditions according to Carey [33] method. We showed that P. plumifer can develop and reproduce on corn pollen under laboratory conditions, so the predator can persist in the field when its main prey is scarce or absent. Survival rate was 97% at immature stages and adult females appeared in 10th day and started laying eggs. On day 16 a sharp decline observed in survival curve and all of individuals died until 20th day (Figure 2). By comparing with Hamedi et al. [34] results we can conclude that corn pollen as lonely food source increases longevity of immature stages and decreases longevity and fecundity of adults of the predator considerably, although the predator can develop and reproduce successfully.
Figure 2.
The age specific survival (lx) and age-specific fecundity (mx) (♀/♀) of Phytoseius plumifer on corn pollen.
2.4.3 Side effect of acaricides on phytoseiid mites with an emphasis on Phytoseius plumifer
Use of pesticides cannot be eliminated in a short period of time in perennial crops because phytoseiid mites, as the most important predators of phytophagous mites, might not be able to maintain the spider mite populations below the economically acceptable level on their own. Therefore successful utilization of biological control agents could depend on the compatibility of the natural predators with pesticides [35]. Most of the phytoseiid mites that naturally occur on plants, even in the absence of tetranychids, are generalist predators [36] and must be preserved using selective plant protection products [37]. Studying the side-effects of pesticides on natural enemies, including predaceous mites is an important task in pest management program, however, the use of pesticides remains necessary due to inadequate control achieved by natural enemies. The combination of biological and chemical control as an IPM program is only possible when the side-effects of pesticides on natural agents are well known [38].
Any indirect effects, which are referred to as sublethal, latent, or cumulative adverse effects may be associated with inhibiting longevity, fecundity, reproduction (based on the eggs laid by females), development time, mobility, prey consumption, emergence rates, and sex ratio and effects of sublethal concentration on the subsequent generation. In our previous study, the subletal effects of two acaricides abamectin (Vermectin_ 1.8% EC, Giah, Iran) and fenpyroximate (Ortus 5% SC, Giah, Iran) on the predatory mite P. plumifer fed on T. urticae was assessed in laboratory conditions. The adult predators were exposed to the residues of these acaricides on fig leaves for LC50 value determination based on a concentration–response analysis. Then sublethal effects of acaricides on performance of treated females and their offspring of P. plumifer were assessed.
2.4.3.1 Materials and methods
The P. plumifer individuals were originally collected from unsprayed (for ten years) fig orchards of Iran. The rearing method were explained comprehensively [34, 39]. All laid eggs were transferred daily from rearing arena to new arenas and were reared to adulthood and then used in the bioassay experiments. Pollen grains and T. urticae were used as food source in rearing and treatment arena.
2.4.3.1.1 Concentration-response bioassay
Concentration–response bioassay was carried out for acaricides using adult females and males at the first day of emergence. A modification of the leaf-dip technique was used [34, 39]. The sublethal concentrations consisted of LC10, LC20 and LC30 were evaluated and used for assessment of sublethal effects on biological performance of P. plumifer [34, 39, 40].
2.4.3.1.2 Sublethal effects of acaricides on biological performance of treated females
Leaf discs with 3.3 cm diameter were treated with sublethal concentrations (LC10, 20, 30) of acaricides and distilled water (as control) and then let to dry. The discs were placed on cotton pads as the same manner as rearing arena [39]. 40 less than 24-h-old unmated females were used in each concentration and stored at 27 ± 1°C, 50% RH and a photoperiod of 16:8 h (L:D). After 72 h treated mites were considered as alive if they were able to move for a distance without losing their balance during the movement and did not turn upside down. The survived females were selected for assessing sublethal effects of acaricides on them. Then each female was exposed to an untreated male from stock colony. Mortality and oviposition were recorded daily until the death of the last female in both treatments and controls. The dead males were replaced with new ones through the experiments.
2.4.3.1.3 Sublethal effects of acaricides on the developmental and biological performance of the offspring from treated females
The eggs laid by the treated and untreated (control) females were collected daily and life-table parameters of both groups were determined and compared to evaluate any possible carry-over activity of acaricides on the offspring. The subsequent generation were checked daily from eggs to dead of the last female. Development time, mortality, oviposition parameters and voracity were recorded daily and life-table parameters were taken until the death of the last female.
2.4.3.1.4 Sublethal effects of acaricides on prey consumption of treated female and the subsequent generation
For assessment of any sublethal effect on prey consumption of treated predators 20 to 30 only protonymphal stage (to decrease the adverse effect of prey webbing on predator) of T. urticae were placed on each treated and untreated (control) leaf disc as predator food source. Forty-eight hours after treatment, an unexposed male from the rearing arena was presented to each surviving female. Males that died during the experiments were replaced. The prey consumption of P. plumifer females was recorded separately for their pre-oviposition, oviposition and post-oviposition periods, because of the different rates for each one, were observed previously in our experiments [39]. Fresh preys were replaced with consumed ones in treated and untreated arena every 24 hours to maintain a constant daily food supply. Through the experiment adult male and female P. plumifer were kept pair. Consumption by the male measured previously as two protonymph per day, which subtracted from the total.
The eggs laid by the treated and untreated females were collected daily and moved to untreated leaf disc for assessment of sublethal effect on prey consumption of P. plumifer treated female’s offspring from nymph to dead of the last female. Depending on the number of eggs, that laid by exposed females, approximately 10 and 30 replications were carried out for abamectin and fenpyroximate treatments, respectively. After emergence of the adults, males and females were paired and male consumption was subtracted as described previously. Individuals were checked daily and the number of protonymphs of T. urticae that had been consumed were counted, recorded and replaced with fresh ones until the death of the last predator. 10, 20, 30 and 20 protonymph stage of T. urticae were provided daily for proto- and deutonymphal stages and the pre-oviposition, oviposition and post-oviposition periods of predators, respectively. This was in excess of that required for daily consumption, as observed by our earlier experiments [39].
2.4.3.1.5 Data analysis
Mortality was corrected by using Abbott’s Equation [41]. The LC50, other sublethal concentrations and the regression equation were evaluated for the dose mortality line were extracted by using a probit program of SAS. The 95% confidence intervals of LC50 obtained from 72 h acute concentration–response curves developed from the responses of adult females and males, for comparing susceptibility of them. Any deviation from the expected sex ratio of 1:1 was determined using a chi-square analysis. For comparing longevity, fecundity, and duration of each stage among different concentrations and the control, analysis of variance (ANOVA) was used. Least Significant Difference (LSD) sequential test was used for comparing the means.
Based on the procedures developed by some authors [33, 42], the following life-table parameters were calculated: gross reproductive rate (GRR), net reproductive rate (R0), intrinsic rate of increase (rm), finite rate of increase (k), doubling time (D), mean generation time (T), intrinsic rate of birth (b) and intrinsic rate of death (d). Jackknife method was used to generate and compare mean demographic parameter estimates with SE values [43]. For comparing life table parameters among different concentrations and controls analysis of variance (ANOVA) was used. The means were compared using LSD sequential test.
2.4.3.2 Results
Our results of several experiments on side effects of acaricides on predatory mite P. plumifer demonstrated that, to evaluate the total effects of acaricides, in spite of effects on treated predator, assessment of all effects on offspring from treated females (subsequent generation) is necessary. Otherwise the real effects of residual exposure on performance of predatory mites would have incomplete end points. Our study proved that abamectin and fenpyroximate had an adverse effect on biological performance of P. plumifer females and their offspring [34, 39, 40]. Many other studies showed these effects on phytoseiid mites too [38, 44, 45, 46, 47].
2.4.3.2.1 Sublethal effects of acaricides on mortality
Reduction in settlement ratio of phytoseiid mites treated by abamectin reported in our study and several other studies too [34, 36, 44]. Our results along with other studies on predators of T. urticae showed that most mortality occurred in 3 days after exposure to abamectin while in the first day there was no effect or a few effects [36, 48, 49, 50]. Abamectin was too toxic for P. plumifer in our study; it caused 100% mortality in female predators in 0.1 concentration that recommended for T. urticae control in the field. Moreover P. plumifer males were more susceptible than females to abamectin and fenpyroximate residue.
2.4.3.2.2 Sublethal effects of acaricides on eggs hatch and sex ratio of subsequent generation
The eggs laid by treated females were hatched at least 96.08% in fenpyroximate treatment so this parameter was not affected significantly. The sex ratio of P. plumifer was affected by fenpyroximate and the treatment caused a reverse in sex ratio. Sex ratio was 16:8 (female:male) in subsequent generation of untreated females that changed to 10:26 (female:male) in subsequent generation of treated females with LC30 of fenpyroximate. Increasing the number of male in comparison with female in subsequent generation of treated female with fenpyroximate can be the other reason of decreasing the predator population after two generations [36, 39]. The sex ratio and egg hatch rate of P. plumifer were not significantly affected by abamectin sublethal concentrations.
2.4.3.2.3 Sublethal effects of acaricides on longevity of females and subsequent generation
Our findings revealed that nymphal periods of offspring of exposed females to acaricides (fenpyroximate and abamectin) were shortened significantly. Moreover, the duration of pre-oviposition, oviposition and post-oviposition periods, and female longevity were significantly affected by sublethal concentrations of acaricides in both treated and their subsequent generation [36, 39]. This is in agreement with another research on Neoseiulus longispinosus (Evans, 1952) [36]. Our results indicated that longevity of treated females and their offspring were adversely affected by abamectin and fenpyroximate treatments. Reduction in female longevity of N. longispinosus after using abamectin, was reported too [36]. We assumed that shortened longevity of both treated females and their offspring may be partially explained by reduced food uptake as a consequence of acaricides effects [40].
2.4.3.2.4 Sublethal effects of acaricides on reproductive performance of females and subsequent generation
Acaricides, abamectin and fenpyroximate caused an overall reduction of P. plumifer population by increasing pre-oviposition period, decreasing oviposition period, decreasing fecundity in both treated female and their offspring. The number of eggs laid by treated female was so affected in both abamectin and fenpyroximate treatment. The total laid eggs were 46.57 eggs in control that decreased to 0.57 and 1.08 eggs in LC20 and LC30 treatment of abamectin and fenpyroximate, respectively. The treated females with LC30 of abamectin laid no egg (Figure 3).
Figure 3.
Effects of sublethal concentrations of acaricides (fenpyroximate and abamectin) on fecundity of Phytoseius plumifer. Different small letters above each bar indicate a statistically significant difference between concentrations. Different capital letters above each bar indicate a statistically significant difference between acaricides (P < 0.05) (LSD).
2.4.3.2.5 Sublethal effects of acaricides on demographic parameters
The intrinsic rate of increase (rm) is based on both survivorship and fecundity. So it has been recommended to use for evaluating the total effects of pesticides [51]. Our results along with several other studies have reported that life-table parameters of phytophagous and predatory mites were affected by sublethal concentrations of acaricides [36, 39, 45, 46, 47]. In our study, the life-table parameters showed significant differences, in population growth and reproductive performance, between offspring from females treated with sublethal concentrations of acaricides (fenpyroximate and abamectin) and untreated females of P. plumifer even in the lowest concentration (LC10). The intrinsic rate of increase (rm), (Figure 4) the net reproductive rate (R0) and the finite rate of increase (λ) of the offspring of treated females with both acaricides were markedly lower compared with the offspring of untreated females. This in turn resulted in a longer doubling time (DT). Moreover, in our laboratory observations the decrease in rm values in sublethal concentrations maybe due to reduction of the mating rate and mobility of the offspring from treated females than untreated ones [36, 39].
Figure 4.
The intrinsic rate of increase (rm) of offspring of the treated and untreated females of Phytoseius plumifer. Different small letters above each bar indicate a statistically significant difference between concentrations. Different capital letters above each bar indicate a statistically significant difference between acaricides (P < 0.05) (LSD).
2.4.3.2.6 Sublethal effects of acaricides on prey consumption of females and the subsequent generation
Our study revealed that prey consumption of treated females were considerably affected by sublethal concentrations of acaricides (abamectin and fenpyroximate) (Figure 5). But these concentrations slightly affected the prey consumption of subsequent generation. Daily prey consumption in the oviposition period was affected more than the other periods in both treated females and their offspring by both of acaricides. Decreasing longevity is another factor that may cause reduction in total prey consumption.
Figure 5.
Total voracity of treated and untreated females of Phytoseius plumifer. Different small letters above each bar indicate a statistically significant difference between concentrations. Different capital letters above each bar indicate a statistically significant difference between acaricides (P < 0.05) (LSD).
3. Conclusion
The low concentrations of pesticides may be used in combination with biological control agents within an IPM system to reduce the selective pressure and development of resistance in pests, but this study showed that adverse effects of fenpyroximate and abamectin on P. plumifer were significant, indicating that this acaricide may not be advisable for combined use with P. plumifer in IPM programs for controlling T. urticae. Even the low concentrations of acaricides that was suggested could be used in combination with biological control agents [52] had considerable adverse effects on this predator.
\n',keywords:"Phytoseiidae, Tetranychus urticae, sublethal dose, abamectin, fenpyroxymate",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/78779.pdf",chapterXML:"https://mts.intechopen.com/source/xml/78779.xml",downloadPdfUrl:"/chapter/pdf-download/78779",previewPdfUrl:"/chapter/pdf-preview/78779",totalDownloads:93,totalViews:0,totalCrossrefCites:1,dateSubmitted:"May 26th 2021",dateReviewed:"September 4th 2021",datePrePublished:"September 27th 2021",datePublished:null,dateFinished:"September 27th 2021",readingETA:"0",abstract:"Spider mites (family Tetranychidae) are important pests of many agricultural, medicinal and ornamental plants worldwide. They possess needle-like chelicerae which pierce plant cells, often feeding on chloroplasts on the under surface of the leaf and cause upper leaf surfaces develop whitish or yellowish stippling. Additionally spider mites produce silk webbing which covers the leaves. In this chapter we present common control methods of these mites including biological control with emphasizing on the prey preference, switching behavior and mutual interference of a biological control agent, Phytoseius plumifer (Canestrini and Fanzago). Additionally the side effects of two acaricides, abamectin and fenpyroxymate, on this predator will be discussed.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/78779",risUrl:"/chapter/ris/78779",signatures:"Samira Khodayari and Nayereh Hamedi",book:{id:"11015",type:"book",title:"Insecticides",subtitle:null,fullTitle:"Insecticides",slug:null,publishedDate:null,bookSignature:"Dr. Ramón Eduardo Rebolledo Ranz",coverURL:"https://cdn.intechopen.com/books/images_new/11015.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-83969-027-3",printIsbn:"978-1-83969-026-6",pdfIsbn:"978-1-83969-028-0",isAvailableForWebshopOrdering:!0,editors:[{id:"193813",title:"Dr.",name:"Ramón Eduardo",middleName:null,surname:"Rebolledo Ranz",slug:"ramon-eduardo-rebolledo-ranz",fullName:"Ramón Eduardo Rebolledo Ranz"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Tetranychidae control Methods",level:"1"},{id:"sec_2_2",title:"2.1 Chemical control",level:"2"},{id:"sec_3_2",title:"2.2 Cultural control",level:"2"},{id:"sec_4_2",title:"2.3 Host plant resistance",level:"2"},{id:"sec_5_2",title:"2.4 Biological control",level:"2"},{id:"sec_5_3",title:"2.4.1 Prey stage preference, switching and mutual interference of Phytoseius plumifer",level:"3"},{id:"sec_5_4",title:"2.4.1.1 Materials and methods",level:"4"},{id:"sec_5_5",title:"2.4.1.1.1 No-choice experiment",level:"5"},{id:"sec_6_5",title:"2.4.1.1.2 Choice experiment",level:"5"},{id:"sec_7_5",title:"2.4.1.1.3 Switching",level:"5"},{id:"sec_8_5",title:"2.4.1.1.4 Mutual interference",level:"5"},{id:"sec_10_4",title:"2.4.1.2 Results",level:"4"},{id:"sec_12_3",title:"2.4.2 Phytoseius plumifer performance feeding on corn pollen",level:"3"},{id:"sec_13_3",title:"2.4.3 Side effect of acaricides on phytoseiid mites with an emphasis on Phytoseius plumifer",level:"3"},{id:"sec_13_4",title:"2.4.3.1 Materials and methods",level:"4"},{id:"sec_13_5",title:"2.4.3.1.1 Concentration-response bioassay",level:"5"},{id:"sec_14_5",title:"2.4.3.1.2 Sublethal effects of acaricides on biological performance of treated females",level:"5"},{id:"sec_15_5",title:"2.4.3.1.3 Sublethal effects of acaricides on the developmental and biological performance of the offspring from treated females",level:"5"},{id:"sec_16_5",title:"2.4.3.1.4 Sublethal effects of acaricides on prey consumption of treated female and the subsequent generation",level:"5"},{id:"sec_17_5",title:"2.4.3.1.5 Data analysis",level:"5"},{id:"sec_19_4",title:"2.4.3.2 Results",level:"4"},{id:"sec_19_5",title:"2.4.3.2.1 Sublethal effects of acaricides on mortality",level:"5"},{id:"sec_20_5",title:"2.4.3.2.2 Sublethal effects of acaricides on eggs hatch and sex ratio of subsequent generation",level:"5"},{id:"sec_21_5",title:"2.4.3.2.3 Sublethal effects of acaricides on longevity of females and subsequent generation",level:"5"},{id:"sec_22_5",title:"2.4.3.2.4 Sublethal effects of acaricides on reproductive performance of females and subsequent generation",level:"5"},{id:"sec_23_5",title:"2.4.3.2.5 Sublethal effects of acaricides on demographic parameters",level:"5"},{id:"sec_24_5",title:"2.4.3.2.6 Sublethal effects of acaricides on prey consumption of females and the subsequent generation",level:"5"},{id:"sec_29",title:"3. 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Sublethal effects of fenpyroximate on life table parameters of the predatory mite Phytoseius plumifer. Biocontrol. 2010;55: 271-278. DOI: 10.1007/s10526-009-9239-4'},{id:"B40",body:'Hamedi N, Fathipour Y, Saber M, Sheikhi Gargan A. Sublethal effects of two common acaricides on the consumption of Tetranychus urticae (Prostigmata: Tetranychidae) by Phytoseius plumifer (Mesostigmata: Phytoseiidae). Systematic and Applied Acarology. 2009;14:197-205. DOI: 10.11158/saa.14.3.4'},{id:"B41",body:'Abbott WS. A method of computing effectiveness of an insecticide. Journal of Economic Entomology. 1925; 18: 265-267.'},{id:"B42",body:'Pielou EC. Mathematical ecology. Wiley, New York; 1977. p. 627-628. DOI: 10.1002/bimj.4710200616'},{id:"B43",body:'Maia AHN, Luiz AJB, Camponhola C. Statistical inference on associated fertility life table parameters using Jackknife technique: computational aspects. Journal of Economic Entomology. 2000;93:511-518. 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Application of demographic analysis for assessing effects of pesticides on the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae). Persian Journal of Acarology. 2021;10(3):281-298. DOI: 10.22073/pja.v10i3.66756'},{id:"B48",body:'Cote KW, Lewis EE, Schultz PB. Compatibility of acaricide residues with Phytoseiulus persimilis and their effects on Tetranychus urticae. Hortscience. 2002;37:906-909. DOI: 10.21273/HORTSCI.37.6.906'},{id:"B49",body:'Chen T, French JV, Liu T, Graca V. Residual toxicities of pesticides to the predaceous mite Galendromus helveolus (Acari: Phytoseiidae) on Texas citrus. Subtropical Plant Science. 2003;55:40-45.'},{id:"B50",body:'Kim DS, Brooks DJ, Riedl R. Lethal and sublethal effects of abamectin, spinosad, methoxyfenozide and acetamiprid on the predaceous plant bug Deraeocoris brevis in the laboratory. Biocontrol. 2006;51:465-484.'},{id:"B51",body:'Stark JD, Wennergren U. Can population effects of pesticides be predicted from demographic toxicological studies? Journal of Economic Entomology. 1995;88:1089-1096. DOI: 10.1093/jee/88.5.1089'},{id:"B52",body:'Dent D. Insect pest management. CABI Publishing, Wallingford, 2th edition. 2000;510 p. DOI: 10.1046/j.1439-0418.2001.0538a.x'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Samira Khodayari",address:"smrkhodayari@gmail.com;, khodayari@maragheh.ac.ir",affiliation:'
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This chapter aims to bring the reader a first-meeting introduction for quickly knowing about MREs, instead of a very deep understanding of MREs.",book:{id:"7685",slug:"smart-and-functional-soft-materials",title:"Smart and Functional Soft Materials",fullTitle:"Smart and Functional Soft Materials"},signatures:"Taixiang Liu and Yangguang Xu",authors:[{id:"283475",title:"Associate Prof.",name:"Yangguang",middleName:null,surname:"Xu",slug:"yangguang-xu",fullName:"Yangguang Xu"}]},{id:"67279",title:"Development, Characterization and Properties of Silk Fibre and Grafted Silk Fibre Reinforced Polymer Composite Films",slug:"development-characterization-and-properties-of-silk-fibre-and-grafted-silk-fibre-reinforced-polymer-",totalDownloads:1107,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"The use of natural fibres over synthetic fibres is gaining widespread importance due to its availability; renewability, low density and satisfactory mechanical properties making them an ecological alternative to synthetic fibres. The innumerable properties of silk fibre have made it superior to be used by researchers both in the plastic and biomedical sector. Silk fibre reinforced PVA (polyvinylalcohol) and PVA/PVP (polyvinyl pyrrolidone) films were prepared via solution casting technique. The effect of silk fibre concentration, on the structural, thermal, mechanical, bio-degradable and the morphological properties of the composite films was assessed. The results indicated that the addition of silk fibres improved the thermal, morphological, mechanical and biodegradable properties of the films. The extensive use of silk fibroin in the biomedical field, due to its robust properties has made it a promising material, suitable in tissue engineering applications. Keeping this in view, the current study also focuses on re-tailoring the properties of silk fibres by grafting a natural polysaccharide like chitosan and thereby fabricate composite films of PVA reinforced with this grafted fibre. The films were tested for their potential applications in tissue engineering, by subjecting them to in vitro biocompatibility tests. The films were also tested for their antibacterial properties. The results thus obtained indicated that the films were non-toxic in all concentrations and were found to be suitable for biomaterial applications.",book:{id:"8162",slug:"generation-development-and-modifications-of-natural-fibers",title:"Generation, Development and Modifications of Natural Fibers",fullTitle:"Generation, Development and Modifications of Natural Fibers"},signatures:"Sareen Sheik and Gundibasappa Karikannar Nagaraja",authors:[{id:"285152",title:"Prof.",name:"G.K.",middleName:null,surname:"Nagaraja",slug:"g.k.-nagaraja",fullName:"G.K. Nagaraja"},{id:"285153",title:"Ms.",name:"Sareen",middleName:null,surname:"Sheik",slug:"sareen-sheik",fullName:"Sareen Sheik"}]},{id:"38395",title:"Structural Health Monitoring for Composite Materials",slug:"structural-health-monitoring-for-composite-materials",totalDownloads:7746,totalCrossrefCites:8,totalDimensionsCites:27,abstract:null,book:{id:"3052",slug:"composites-and-their-applications",title:"Composites and Their Applications",fullTitle:"Composites and Their Applications"},signatures:"Jian Cai, Lei Qiu, Shenfang Yuan, Lihua Shi, PeiPei Liu and Dong Liang",authors:[{id:"140597",title:"Dr.",name:"Jian",middleName:null,surname:"Cai",slug:"jian-cai",fullName:"Jian Cai"},{id:"140715",title:"Prof.",name:"Shenfang",middleName:null,surname:"Yuan",slug:"shenfang-yuan",fullName:"Shenfang Yuan"}]},{id:"69714",title:"Natural Fibers: Applications",slug:"natural-fibers-applications",totalDownloads:1668,totalCrossrefCites:4,totalDimensionsCites:7,abstract:"Fibers derived from bio-based sources such as vegetables and animal origin are termed as natural fibers. This definition includes all natural cellulosic fibers (cotton, jute, sisal, coir, flax, hemp, abaca, ramie, etc.) and protein-based fibers such as wool and silk. There are also man-made cellulose fibers (e.g., viscose rayon and cellulose acetate) that are produced with chemical procedures from pulped wood or other sources (cotton, bamboo). Natural fibers being cost effective and abundantly available yields high potential in various industrial and commercial applications such as in the interior applications of the passenger cars, panels for partition and false ceiling, partition boards, roof tiles, coir fibers in packaging, furniture applications, as insulating materials in low energy houses, geo-textiles for soil protection and erosion control, enhancing barrier properties, composites etc. Due to research and developmental work in modification and treatment methods of natural fibers, utilization of natural fibers has observed a significant growth in various applications. The chapter addresses the potential applications of natural fibers in various commercial sectors for the development of environment-friendly products with an aim to replace synthetic fibers or inorganic fillers with cost-effective and efficient products.",book:{id:"8162",slug:"generation-development-and-modifications-of-natural-fibers",title:"Generation, Development and Modifications of Natural Fibers",fullTitle:"Generation, Development and Modifications of Natural Fibers"},signatures:"Jatinder Singh Dhaliwal",authors:[{id:"272683",title:"Mr.",name:"Jatinder Singh",middleName:null,surname:"Dhaliwal",slug:"jatinder-singh-dhaliwal",fullName:"Jatinder Singh Dhaliwal"}]}],onlineFirstChaptersFilter:{topicId:"934",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. His teaching areas are energy metabolism and regulation, integration and organ specialization and metabolic adaptation.",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null},{id:"18",title:"Proteomics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",isOpenForSubmission:!0,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null}]},overviewPageOFChapters:{paginationCount:36,paginationItems:[{id:"82195",title:"Endoplasmic Reticulum: A Hub in Lipid Homeostasis",doi:"10.5772/intechopen.105450",signatures:"Raúl Ventura and María Isabel Hernández-Alvarez",slug:"endoplasmic-reticulum-a-hub-in-lipid-homeostasis",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Endoplasmic Reticulum",coverURL:"https://cdn.intechopen.com/books/images_new/11674.jpg",subseries:{id:"14",title:"Cell and Molecular Biology"}}},{id:"82409",title:"Purinergic Signaling in Covid-19 Disease",doi:"10.5772/intechopen.105008",signatures:"Hailian Shen",slug:"purinergic-signaling-in-covid-19-disease",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82374",title:"The Potential of the Purinergic System as a Therapeutic Target of Natural Compounds in Cutaneous Melanoma",doi:"10.5772/intechopen.105457",signatures:"Gilnei Bruno da Silva, Daiane Manica, Marcelo Moreno and Margarete Dulce Bagatini",slug:"the-potential-of-the-purinergic-system-as-a-therapeutic-target-of-natural-compounds-in-cutaneous-mel",totalDownloads:10,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82103",title:"The Role of Endoplasmic Reticulum Stress and Its Regulation in the Progression of Neurological and Infectious Diseases",doi:"10.5772/intechopen.105543",signatures:"Mary Dover, Michael Kishek, Miranda Eddins, Naneeta Desar, Ketema Paul and Milan Fiala",slug:"the-role-of-endoplasmic-reticulum-stress-and-its-regulation-in-the-progression-of-neurological-and-i",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Endoplasmic Reticulum",coverURL:"https://cdn.intechopen.com/books/images_new/11674.jpg",subseries:{id:"14",title:"Cell and Molecular Biology"}}}]},overviewPagePublishedBooks:{paginationCount:32,paginationItems:[{type:"book",id:"7006",title:"Biochemistry and Health Benefits of Fatty Acids",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7006.jpg",slug:"biochemistry-and-health-benefits-of-fatty-acids",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Viduranga Waisundara",hash:"c93a00abd68b5eba67e5e719f67fd20b",volumeInSeries:1,fullTitle:"Biochemistry and Health Benefits of Fatty Acids",editors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",middleName:null,surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. Waisundara",profilePictureURL:"https://mts.intechopen.com/storage/users/194281/images/system/194281.jpg",biography:"Dr. Viduranga Waisundara obtained her Ph.D. in Food Science\nand Technology from the Department of Chemistry, National\nUniversity of Singapore, in 2010. She was a lecturer at Temasek Polytechnic, Singapore from July 2009 to March 2013.\nShe relocated to her motherland of Sri Lanka and spearheaded the Functional Food Product Development Project at the\nNational Institute of Fundamental Studies from April 2013 to\nOctober 2016. She was a senior lecturer on a temporary basis at the Department of\nFood Technology, Faculty of Technology, Rajarata University of Sri Lanka. She is\ncurrently Deputy Principal of the Australian College of Business and Technology –\nKandy Campus, Sri Lanka. She is also the Global Harmonization Initiative (GHI)",institutionString:"Australian College of Business & Technology",institution:null}]},{type:"book",id:"6820",title:"Keratin",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6820.jpg",slug:"keratin",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Miroslav Blumenberg",hash:"6def75cd4b6b5324a02b6dc0359896d0",volumeInSeries:2,fullTitle:"Keratin",editors:[{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. 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He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,series:{id:"11",title:"Biochemistry"}}},seriesLanding:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:4,numberOfPublishedChapters:318,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). 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Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. 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Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. 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Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",annualVolume:11414,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",fullName:"Shymaa Enany",profilePictureURL:"https://mts.intechopen.com/storage/users/81926/images/system/81926.png",institutionString:"Suez Canal University",institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"chapter.detail",path:"/chapters/41987",hash:"",query:{},params:{id:"41987"},fullPath:"/chapters/41987",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()