\r\n\tCKs have crucial roles in various viral infections such as influenza, hepatitis B virus (HBV), hepatitis C virus (HCV), viral meningitis, human immunodeficiency virus (HIV), and SARS-CoV-2. \r\n\tCKs mediate the directing of the transport of leukocyte cells into the tumor microenvironment to generate the host response against cancer. CKs can directly modulate tumor tissue expansion by inducing the proliferation of cancerous cells and inhibiting their apoptosis. They can also indirectly modulate the growth of tumor tissue through the effects of CKs on tumor stromal cells, by inducing the release of growth and angiogenic factors of cells that make up the tumor microenvironment.
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1. Introduction
The fast changes in soil use and higher vegetal resource demands favor the triggering of water erosion that needs to have its rates expressed in space and time for the proper adaptation of control practices and resources for agricultural planning. The physical processes of disaggregation, transport and soil deposition that define the erosive process are hydrologically directed and the movement of the water on the soil undergoes the interference of the topography, climate, soil class and land use, so that the studies regarding the theme are based on the intense experimentation of the effects of the variations of these factors on the sediment production. The estimate of the topographic variables, although benefitted by automatic generation and spatial distribution made possible by the Geographical Information Systems (GIS’s), is the target of controversy related to the formulation of algorithms for this end, so that its three-dimensional calculation is not a current procedure in the geoprocessing programs [1].
Given the scope of the process, the topographic modeling in the erosion analyses can differ in terms of complexity, processes considered and data required for model use and calibration, which can be empirical, physical and conceptual [2]. In the empirical models most used, such as USLE (Universal Soil Loss Equation) [3] and the revised version of USLE (Revised Universal Soil Loss Equation - RUSLE) [4], the topographic factor is expressed by the association of the steepness and the length of the slope called, respectively, factors S and L. Considering the proper formulation of USLE and its adaptation to the work context in the Digital Elevation Model (DEM), obviously the advantages associated to DEM derive almost entirely on issues related to the topographic LS factor, because it can be evaluated with the aid of DEM and where the precision of the extracted parameters can become apparent [5].
An aspect that hinders the estimate of appropriate topographic factor (LS) values for applications in GIS and results in high limitation in the use of the USLE and RUSLE erosion models [6, 7] are the dynamics of the erosive process in complex reliefs and hydrographic basins, since USLE was primarily developed for the prediction of the erosion in not very accentuated and uniform slope stretches, in other words, not considering if they are concave, convex [8], or in combination. The limitation of the empirical modeling in the soil loss estimates in complex profiles impelled the development of conceptual models (or semi-empirical), such RUSLE 3D and USPED (Unit Stream Power Erosion and Deposition) [9]. Derived from USLE, these models intend to represent their advancements by adding a physical basis that tries to relate the morphology of the relief and the erosion defining parameters.
In this sense, given the strategic need for generation and diffusion of algorithms for automatic mapping of the topographic variables used in the operationalization of the digital analyses of water erosion, the objective of the present chapter was to conduct a review of the topographic factor development in erosion equations applied in computational geoprocessing systems, with prominence for USLE and RUSLE, addressing the main theories and algorithms used in the digital treatment of the data.
2. Digital Elevation Model (DEM)
In the landscape, the topography determines the behavior of the surface runoff, the phase of the hydrologic cycle that is most directly associated to the water erosion and that requires a rigorous and effective analysis throughout its entire extension, make possible with the use of digital elevation models (DEM). The analyses developed on a DEM allow: to visualize the model in planar geometric projection; generating gray scale images, shaded images and thematic images; calculating fill (embankment) and cut volumes; conducting profile analyses on predetermined trajectories; and generating derivative maps, such as steepness and exposure maps, drainage maps, contour maps and visibility maps. Products of the analyses can even be integrated with other geographical data types aiming at the development of several geoprocessing applications, such as urban and rural planning, agricultural suitability analyses, risk area determination, environmental impact report generation [10], elaboration of digital soil maps, as well as maps of soil attributes such as soil organic matter content [11], among others. Therefore, DEM should faithfully represent the relief allowing to capture the topographic variations presented.
The elaboration and creation of a DEM, indispensable for the representation of a real surface on the computer, can be represented by analytical equations or a network (grid) of points, in a way that transmits the spatial characteristics of the land to the user [12]. Therefore, the information contained before in specific points (vectors) are transformed into a continuous spatial distribution of the relief (raster), enabling new inferences about the local relief. Different methods exist for the interpolation of the data and DEM generation, which are built through regular rectangular grids, such as the Topogrid [13], or triangulated irregular networks (TIN) [14]. For the choice of efficient DEM in the evaluation of the erosive process, an intense preliminary analysis of information, from a hydrologic point of view, is recommended, because the development of the water erosion occurs in response to the manner the water moves through and on the landscape [15].
The geomorphological and hydrological consistency of a DEM is reached when the matrix image faithfully represents the relief features, such as the hydrographic basin watershed, thalwegs and concave and convex elements, and it assures the convergence of the surface runoff for the mapped drainage network. In this sense, several water erosion analysis and modeling works have used the TIN model [16-18], as well as the Topogrid model [19-21] for DEM generation. In a research conducted with the objective of defining the drainage network in a sub-basin [22], the original contour curves (scale 1:10.000) were compared to the curves generated by DEM\'s of the Topogrid, linear TIN and TIN natural neighbor interpolators. A higher Topogrid hydrological consistency was observed verified in the better continuity of the contour curves and higher drainage area and watershed detailing, resulting in a smaller amount of flat areas and in more detailed drainage pathways. The authors emphasized that all of the generated models have high reliability due to the precise topographic surveys data from which they originated, a fact also observed by [15, 18]. Starting from this same comparison among interpolators, other works [23, 24] made similar observations regarding the behavior and reliability of the models.
The precision of the data collection will influence the quality of the corresponding digital model and the choice of the database to be used in the construction of DEM becomes fundamental. Such data can be derived from contour curves, elevation points, photogrammetric analysis from aerial photography, information collected by stereoscopic satellite images, or radar [25, 26]. The digital database can result from a digitized manual survey obtained through direct readout digital equipment (total stations, topographic GPS), or by remote sensing equipment (radar, laser) in which different DEM generation models can be applied supplying DEM\'s with varying precision [26]. The spatial distribution and the amount of errors propagated can vary according to the spatial resolution, therefore, the effect of the spatial resolution on supplying useful information for the determination of an appropriate resolution should be investigated [27]. The higher the scale of a map, the higher detail it presents, and to the contrary, the lower the scale in a map, the higher the degree of generalization seen which increases the minimum cartographic area of the land [27]. With better horizontal resolution DEM’s, it is possible to include more relief roughness aspects, reducing the length of the slope straight line segments and increasing the accuracy of the L and S factors [18].
The evolution of the geographical information systems and the growing availability of better quality radar images enabled the obtaining of terrain elevation models (DEM) with increasingly better spatial resolutions. However, data surveys considered as having high precision (below 10 meters of resolution) still possess high costs, limiting their use in research. Currently, the most widely used digital database for the generation of DEM’s originates from of the digitization of topographic maps or those obtained by remote sensing maintaining their original precision. Because the influence of the pixel size has a significant weight in the analyses derived from DEM’s, the choice of the spatial resolution proportional to the scale of the primary data must have certain considerations, among them, the original contour curve scale and the characteristics of the mapped relief. For instance, with a minimum horizontal distance between the curves on the order of 20 m, the spatial resolution of 15 m can be shown appropriate for the detailing of the relief presented in the original base, considering that lower resolutions would tend to generate erroneous information (nonexistent), while higher resolutions would not detail the relief in a satisfactory way [11]. Furthermore, a sufficient spatial resolution cannot only depend on the aimed information and/or the precision used in the collection of this information, but also on the topography. In areas where simple hillsides exist with flat topography, a coarse resolution (> 20 m) may not lead to major errors in hydrographic basins, being able to be used with little uncertainty. In a complex topography, with accentuated slopes, a coarse resolution can result in great uncertainty, and a better resolution could be necessary.
Along those lines, studies have been developed seeking to define the best DEM resolution that is capable of precisely representing the variations of the relief, thus reducing the uncertainties of the erosion prediction models that need topographic modeling. In Slovakia, the S factor derived from a MED with 50 m resolution, obtained from digitized contours of the topographic maps in a 1:50.000 scale, promoted a sufficient level of detail for this type of regional evaluation and the spatial resolution selected reflected the scale of the primary data [28]. To determine the resolution of DEM when the objective is to promote an impartial average global estimate, the global variance of the LS factor can be used [29].
The local variance of a cell measure the average local space variability, while the global variance measures the global variation of the estimates showing a considerable difference of behavior with the increase of the DEM resolution. Thus, in [29] the objective of the research was to evaluate the appropriate DEM resolution for the spatial prediction of the LS factor. A decrease of the global variation of the LS values obtained in the 30 by 100 meter resolution in DEM was observed, followed by a stabilization after 100 m. The local average variance and semivariance of a cell increased with the 30 by 50 m resolution and it decreased after 50 m. The highest local variance and semivariance of a cell was at the 50 m resolution and thus it could be considered appropriate for the necessary detailing of the spatial information (distribution and variability) for the LS factor prediction [29].
A study developed in Thailand analyzed the influence of the spatial resolution on the results of the LS factor [30]. Two DEM resolutions extracted from a SRTM (Shuttle Radar Topography Mission) radar image with 90 m of resolution (original image resolution) and 30 m (resampling of the 90 m resolution) were appraised. The grid size change affected the steepness values, compromising the L and S factor values, since the L factor depends on the grid size and the steepness and the S factor only on the steepness. When affecting the L and S factors, the resolution also affected the sediment transport ratio. The best sediment production estimates were observed in DEM with resolution of 30 m. A fundamental observation is made by the authors of the study, who highlight that the better results of the 30 m resolution compared to the 90 m using the USLE methodology, is probably due to fact this resolution is closer to the 22.4 m slope length, the length used in the derivation of the USLE relationships. Another study [31] compared SRTM radar images with spatial a resolution of 90 x 90 m and digitized hypsometric curves to determine the topographic factor. The highest detail was obtained by the SRTM images that evidenced lower LS ranges. They concluded that the difference occurred due to the higher detailing in plane areas, where LS is lower (ramp height lower than 40 m, with low steepness).
3. Water erosion modeling
3.1. USLE and RUSLE empirical modeling
The empirical models are the simplest ones, generally possessing less data and lower computational base than the physical and conceptual ones. The empirical models normally have a high aggregation of time and space and are based on analyses of the erosion process using statistic techniques. For this reason, they are particularly useful as the first step to identify the sediment sources. Universal Soil Loss Equation (USLE) and Revised Universal Soil Loss Equation (RUSLE) have been the most used models in the world for predicting erosion processes due to their simplicity and the availability of information.
The topographic factor is the most sensitive parameter of USLE/RUSLE in the soil loss predictions, where a higher relative effect of the steepness factor is observed in a simple analysis of sensitivity. However, an interaction of the steepness (S) and the slope length (L) exists and, LS being used as the “only” parameter in the sensitivity analysis, its influence is even higher on the soil loss than the remaining parameters, including L and S individually [32]. By definition, the slope length (L) is the distance from the point of origin of the surface flow to the point where each slope gradient (S) decreases enough for the beginning of deposition or when the flow comes to concentrate in a defined channel [3]. The soil losses increase with the increase of the slope length and steepness, conditions where the surface flow reaches high-speeds.
The procedure to obtain the slope length was originally manual in these models, which may be adapted to GIS framework. It initially consists of slope length identification through information plans of slope steepness and classified aspects, such as the exposition angle between the slopes and the north. From each rill slope or polygon, the average slope steepness (degrees) and the altitude are calculated [33, 34]. It is possible to calculate the slope length through the following equation [33]:
L=DHsinαE1
Where L is slope length (m); DH is altitude difference (m); and α is average slope steepness (degrees).
The slope angle α corresponds to the inverse of the tangent angle that may be calculated dividing sin α (altitude difference) by cos α (distance between level curves and/or quoted points). The α angle of a surface defined by two points (A and B) is calculated with the horizontal as show in Figure 1 [35].
Figure 1.
Trigonometric variables in the calculation of the slope.
The slope length (L) and slope steepness (S) factors of the USLE were developed for uniform slopes based on empirical models, which means that they use dependent field measurements. For USLE/RUSLE, they are calculated from the comparison with a ramp length of 22.1 m and 9% slope with the use of a factor m for different steepness classes [3].
The L factor can also be obtained by pixel size in the DEM. If one size of the pixel is considerate as flow length, an equal value is determined for the flown length; therefore it is assumed that the inclination is composed by segments of equal dimensions, consequently with different inclinations, which is not true. However, this approach is considerably feasible if a pixel of suitable dimension is used [36]. A study developed in Thailand evaluated two DEM resolutions and observed better results from 30 m resolution using USLE methodology due to the fact that this one is closer to 22.1 m slope length, which is used for the derivation of model relations [30].
The calculation methodology of LS factors proposed by the USLE was improved in the equation revision, named RUSLE [4], considered more extensive than the previous model. The L factor, in both USLE and RUSLE, is expressed as [3]:
L=(λ22.1)mE2
Where L is slope length effect on soil loss standardized for 22.1 m length; λ is field slope length (m); and m is slope length exponent.
In the USLE, the m recommend value is from 0.2 to 0.5 for slope levels lower than 1%; 1-3%; 3.5-4.5%; and 5% or more, respectively. Therefore, if a slope gradient is higher than 5%, slope length factor do not change with slope inclination. However, in the RUSLE, m continues to increase with slope inclination (Equation 3). Thus, in the RUSLE, the slope length effect is a function of the erosion ratio of rill to interrill [36].
m=β(1+β)E3
β=(sinθ0.0896)[3(sinθ)0.8+0.56]E4
Where β is ratio of rill to interrill erosion; and θ is slope angle.
Researchers observed that the m = 0.5 exponent of USLE is better adapted for very accentuate slopes [37]. When the slope increases from 9% to 60%, the m exponent increases from 0.5 to 0.71. The slope length exponent, m, is 0.7 for a 50% slope with 60 m length and a more moderated ratio of rill and interrill erosion. When the 0.7 factor is used, the RUSLE predicts an addition of 22% of soil loss than the USLE (m = 0.5) through a 60 m of length slope. When the slope is lower than 9%, the USLE will predict a higher soil loss than RUSLE and, being steeper than 9%, the RUSLE will predict a higher soil loss than USLE. The higher difference occurs in much accentuated slopes.
The equation used in the USLE slope factor (S) (Equation 5) was modified to obtain more accurate results in RUSLE model (Equation 6), probably due to changes in the slope factor [34, 38], which depends on the slope angle Ө.
S=65.4sin2θ+4.56sinθ+0.0654E5
S=10.8sinθ+0.03,forθ<9%orS=16.8sinθ−0.50E6
3.1.1. USLE and RUSLE limitations
The restrictions of the USLE and RUSLE empirical models frequently occur because neither examines the hydrologic phenomena in their geographical context, using a simplified representation of spatial elements that assumes the hydrographic basin as uniform [39]. Many methods have been developed seeking to include complex slopes, common in a context of hydrographic basins [40]. In a comparison of several manual methods it was concluded that there is no obviously better method [41]. The errors of the empirical models are produced because the water erosion, being a hydrologically driven process, is not evaluated in relation to the surface runoff [42-45]. In the soil loss estimates using the USLE and RUSLE models the surface runoff is not considered in a direct way, though they indirectly consider that the flow transports the eroded sediment and the concentration of sediments depends on the kinetic energy level of the rain, in the sample space of a parcel [44]. Thus, the surface runoff in the empirical models is a primitive factor. This presupposition limits the potential of these models in predicting erosive factor changes, on the scale of basins or drainage systems, which are favored in models based on physical and semi-empirical processes where the surface runoff constitutes a fundamental factor in the water erosion prediction.
For local conservation planning, the LS factor is usually estimated or calculated from length and inclination measurements in the field [6], or even through manual procedures on cartographic bases, making the procedure very difficult and slow due to the difficulty of individualization of each slope [1]. The measurement of the ramp length is made from the evaluated point in relation to the watershed. Besides possessing the difficulty of locating the watershed, this procedure considers the straight line distance until the watershed, concealing the importance of the relief form, because the erosion is affected by the torrent that comes from the whole contribution area. These labor intensive in-field measurements rendered the soil erosion modeling obviously unviable on a regional scale [6] leading to the determination of the ramp length based on the estimate of an average value for hydrographic basins, which is an oversimplification of the true situation [7]. Furthermore, an underestimate of LS values, obtained manually, and consequently also of the erosion risk is observed when compared to the irregular slopes considered in automated models [46].
A second shortcoming of these models is the evaluation of only the erosion, without sediment deposition prediction [47]. When adopting an average rate for an entire slope or hydrographic basin, addressing the erosion using the USLE and RUSLE models does not offer any information as to the sources and sinks of the erosion materials. In spite of the methodology of dividing complex landscapes into series of semi-homogeneous planes used by these models, to provide some consideration as to the convexity and concavity of the inclination, the erosion is only calculated along the flow in a rectilinear manner, without full consideration of the convergence and divergence flow influence [45]. No approach adequately supplies spatially distributed information on the erosion necessary for effective control of the erosion and sediments. Thus, on a hydrographic basin or landscape scale, the spatial distribution of the soil erosion predicted by such models will distort the current conditions and will tend to overestimate the erosion [47, 48]. Some studies mention overestimates in lower soil losses and underestimates for the high losses using the USLE and RUSLE models [44, 49, 50]. As a solution, studies recommend to first identify those portions of the landscape subject to the deposition and to exclude them from the analysis when applying the USLE and RUSLE models [9].
USLE was related to GIS due to the advantages of handling great amounts of spatial data. Until the middle of the 1990’s, a great limitation in the use of the USLE and RUSLE erosion models on a regional landscape scale was the difficulty in estimating appropriate LS factor values for applications in GIS [7], since such models evaluate the effects of the topography on the erosion in a two-dimensional way. In that context, the use of models distributed in space came to represent a powerful environmental analysis tool, highlighting soil erosion by water on the hydrographic basin scale.
3.2. Conceptual modeling
The conceptual methods incorporate the impact of different erosive processes through empirical parameters [51] usually obtained through calibration with observed data, such as flow discharge and sediment concentration [52]. Therefore, these models represent the processes within the scale in which they were simulated [53]. It is noteworthy, particularly on a large scale, to mention that deposition patterns and sediment residence time are still little understood in a way that the erosion prediction and the sediment deposition rates on these scales are based, usually, on empirical or semi-empirical studies that are applied in a uniform way throughout the whole area [54].
The semi-empirical LS factor explains the double phenomenon of drainage convergence and furrow [27]. The result of the LS factor thus comes to be equivalent to the traditional LS factor on flat surfaces, but with the advantage of being applicable to slopes with complex geometries [55-57]. When substituting the empirical topographic factor by the semi-empirical one in USLE, the laminar and concentrated flow in complex terrains is considered in the spatial distribution of the erosion, making the estimate more precise.
In the conceptual models the slope length factor is substituted by the upstream contribution area [9, 46, 55, 56] whose modeling conducted in the digital elevation model (DEM) allows to determine the drainage network considering the direction of the surface runoff and the accumulated flow. For each cell, the contribution area upstream is obtained from DEM initially calculating the steepness and aspect maps, building the water flow paths later. The upstream contribution area map is determined from the water flow path lines and the DEM spatial resolution. The precision of the model is related to the uncertainty of the empirical parameters used in the LS factor equation, the accuracy and resolution of DEM and to the methods for derivation of the topographical variables related to LS, such as steepness, aspect and contribution area [27]. In that way, the topographical LS factor can be finally obtained.
Incorporating this concept, an equation modified to compute the LS factor in the form of finite difference in a grid of cells representing a segment of the hillside was derived [46]. Another model, called RUSLE 3D (Revised Universal Soil Loss Equation 3D) presented a simple and continuous form of the LS factor equation considering the impact of the convergent flow [9]. Also considering the contribution area, the USPED model (Unit Stream Power Erosion and Deposition) was developed from the drainage force unit theory [56, 57] for analysis of the erosion and deposition.
3.2.1. Contribution area modeling
The modeling of the contribution area is conducted resorting to DEM, because it contains information that allows to determine the surface runoff network. As such, based on DEM, the flow direction and the accumulated flow and the steepness are determined. The area of contribution of each cell (pixel) of DEM, considering a grid of cells, is its own area plus the area of the upstream neighbors that possess some drained fraction for the pixel in question. The contribution area (A) of a specific grid of cells is calculated from the product of the accumulated flow (χ) and the area of each cell (η) [58]:
A=χηE7
The determination of the accumulated drainage areas (or accumulated flow), which allows the simulation of the hydrographic network, are defined based exclusively on the flow directions. The accumulated flow represents the amount of rain that will drain through each cell, supposing that all of the rain become torrents and there is no interception, evapotranspiration, or loss of underground water. Each pixel receives a value corresponding to the sum of the areas of all of the pixels whose drainage contributed to the analyzed pixel [59].
The flow direction defines the flow path of water, as well as sediments and nutrients, in areas adjacent to the lower altitude points in all of the positions in the hydrographic basin [60]. Independent of the magnitude of the rain event, the flow algorithm in a GIS establishes a one-dimensional flow network connecting each cell with other cells of the hydrographic basin in DEM until the point where the whole surface runoff generated inside the hydrographic basin meets, defined as the mouth [61]. As such, the hydrological relationships are built between different points within a hydrographic basin, topographical continuity being necessary so that functional drainage exists [62].
The estimate of the flow direction is based on the physical principle that the mass of controlled gravity proceed in the direction of the most accentuated slope. The slope is characterized identifying the plane tangent to the topographical surface in the center of the cell. The maximum plane elevation change rate characterizes the inclination gradient, while the correspondent cardinal direction of this larger difference is the aspect [63] (Figure 2).
Figure 2.
Code of flow direction in analogy to cardinal points generated on the aspect map.
Many algorithms have been developed to consider the contribution area. The flow direction methods, profoundly different, are classified in: concentrative, also called single direction or eight directions, that transfer the whole source pixel matter to the downstream pixel in question [59, 64-68]; and dispersive or multiple direction, that divide the matter of the source cell among several receptors [63, 66, 69-73]. That means that, while those of single flow consider all of the slopes as concave or parallel, the multiples also differentiate the convex ones [46]. In water erosion analysis, the most thoroughly used methods are the concentrative [1, 7, 29, 47, 74-81].
The first and simpler method to specify the flow direction attributes the flow of each pixel to one of their eight neighbors, be it adjacent or diagonal, in the direction of the steepest hillside slope. This method, designated Deterministic 8 Algorithm (D8), is based on the fact that the water can move in 8 possible directions, as demonstrated in Figure 2 (8 flow directions) [64]. The D8 approach has disadvantages arising from the determination of the flow distributed equally in only one of the eight possible directions, separate by 45˚, that is expressed in parallel (or convergent) flow patterns in the directions of the cardinal or diagonal points, intermediate values not being possible [60]. In a complex topography, however, the divergent flow frequently can occur causing a significant impact on the delimitation of the basin contribution area [80].
Is suggested to overcome these random flow direction attribution problems for one of the descending neighbors, with the probability proportional to the slope [65]. Other flow direction methods [69, 70] have also been suggested in an attempt to solve the limitations of D8. These attribute a fractional flow to each smaller neighbor, proportional to the slope (or, in the case of the Freeman method, inclination to an exponent) for that neighbor. The multiple flow direction method, here designated by MS (based on multiple slope directions), have the disadvantage that the pixel flow is dispersed for all of the neighboring pixels with lower elevation.
An algorithm was developed using the aspect associated to each pixel to specify the flow directions [71]. The flow is directed as if it was a ball rolling on a plane, liberated from the center of each cell grid. This plane is suited to the elevation of the corners of the pixel, and such corner elevations are estimated by the average of the elevations of the central elevation of the adjacent pixels. This procedure has the advantage of continually specifying the flow direction (an angle between 0 and 2π) without dispersion. Extending the ideas of the previous methodology, a group of elaborated procedures was presented called Demon [66]. Gridded elevation values are used as pixel corners, instead of limiting to the center, and a plane surface is formed for each pixel. The authors recognize the flow as two uniform dimensional origins along the area of the pixel, instead of flow paths drawn from the center of each pixel. The upstream area is evaluated through the construction of detailed flow tubes. It is presupposed that a local plane adjustment for each pixel requires approximation because only three points are necessary to determine a plane. The best adjustment plane, in general, cannot cross the four elevations in the corners, leading to a surface representation discontinuity on the edges of the pixel. The local plane adjustment for specific combinations can lead to inconsistent or deduced flow directions that are a problem in the Lea and Demon methods [63].
Being such, a new procedure to represent the flow directions and calculation of the upstream areas using a grid based DEM was suggested, see reference [63], called infinite D or D∞. The D∞ method calculates the water flow direction according to the steepness of the terrain, distributing the flow proportionally among the neighboring cells. This procedure continually specifies the flow direction (an angle between 0 and 2π) taken in the most accentuated hillside slope, distributing it among the eight facets generate by a 3 x 3 pixel mesh that contains the analyzed pixel in the center. These facets avoid the approximation involved in the plane adjustment and the influence of neighbors with higher altitudes on the upstream water flow [82]. When the direction does not follow one of the cardinal (0, π/2, π, 3π/2) or diagonal (π/4, 3π/4, 5π/4, 7π/4) directions, the accumulated flow is calculated from the flow contribution of a pixel between the two upstream pixels according to the proximity of the flow angle in relation to a right angle for the central pixel. A great advantage of the method D∞ is in considering the form of the divergent surface, in other words, the flow also becomes divergent [83]. Comparing results of the statistical tests and map influence and dependence analysis on the calculation of the upstream area in DEM, a better performance of the D∞ method was observed in relation to the D8, MS and Lea methods, being comparable to Demon, but overcoming its problems of frequent inconsistencies [63].
On defining the drainage directions, it is expected that the resulting drainage network is located within the river channel. Depending on the method used, significant DEM differences in the distribution of the contribution area are obtained. Figure 3 presents the accumulated flow map using the D8 and D∞ methods. As the D8 method routes the whole flow to the cell of higher gradient, rectilinear drainage lines are observed. In turn, the D∞ method, since it considers a proportional distribution among the pixels according to the steepness, does not present the characteristic angular tracings of the flow path restriction.
Using low and high resolution DEM assay data examples, differences are more notable with the increase of the DEM data resolution, especially on the slopes scale [63]. Furthermore, the drainage direction determination methods can produce different results that do not always agree with the reality, mainly when applied in plane areas [60, 84, 85], because they depend on the treatment that each algorithm gives to these regions.
Figure 3.
The accumulated flow by the D8 and D-infinity (D∞) methods. (Source: Elaborated by the authors).
Seeking to define a hydrologically consistent digital elevation model and to obtain a sub-basin drainage network, a study [22] tested the D8 [64] and D∞ [63] methods. In the analysis of the mean error between the observed and estimated drainage, the D∞ method provided higher drainage pathway detailing and agreement among the drainage networks. The D8 method provoked errors in the orientation of the drainage network matrix.
The inability of the single direction method (D8) to simulate the flow direction along the inclination of the hillside was also noted [60], which emphasized best performance of the multiple direction method (D∞). The same observations were made in erosive process spatial distribution analysis studies [20, 83, 86-88]. These results were even verified in a study whose purpose was the modeling of the topographic factor [46], which opted for the multiple flow direction method due to its better adjustment in the erosive process analyses.
3.2.2. Desmet & Govers algorithm
The great disadvantage observed in the USLE/RUSLE models is the two-dimensional evaluation used to determine the effects of the topography. In these models, the landscape has been generically treated as homogeneous, with plane characteristics. The first research that developed a procedure for the soil loss calculation capable to consider the slope form divided the irregular slopes into a limited number of uniform segments was [89]. Continuing this study, weights were attributed for the slope stretches according to their convexity or concavity [3].
Extending the study of [89], the upstream contribution area concept was introduced for the calculation of the L factor [46] that was applied to the RUSLE LS factor equations [90, 91]. For the calculation of the contribution area, a multiple flow direction algorithm was used [70]. The L factor is expressed according to the equation:
Where Li,j is the slope length factor of a cell with coordinates (i, j); Ai,j-in is the contribution area of a cell with coordinates (i, j) (m2); D = is the cell grid size (m); xi,j is the flow direction value, obtaining the equation x = senα+ cosα, where α is the flow direction angle; m is the coefficient that assumes the values: 0.5, if S ≥ 5% (S the steepness degree); 0.4, if 3% ≤ S < 5%; 0.3, if 1% ≤ S < 3%; and 0.2, if S < 1%.
For the steepness calculation, the following algorithm was employed [92]:
Gij=Gx2+Gy2E9
Where Gx and Gy are, respectively, the gradient in the direction x (m/m) and the gradient in the direction y (m/m).
The LS factor for a grid of cells can be thus obtained by the insertion of Li,j and Gij in the LS factor equations of the chosen USLE or RUSLE approach.
This algorithm makes calculations of the steepness, flow direction and the amount of flow that accumulated upstream from a pixel for each pixel [46]. As such, the pixel to pixel topographical factor is calculated along complex slopes. As result, it is possible to define where there is significant distance from the watershed and where there is flow convergence (concave slopes), as well as high steepness, where the LS value tends to be high. In compensation, that value is low in the interfluves (hill tops and plateaus), because the slope length and the steepness are reduced.
The method is not limited to express the sediment transport capacity by the runoff, but it also considers the surface flow, the ramp geometry - if concave or convex - and the erosion way [94]. Comparing the results obtained in the automatic method [46] and manual one [3], a research work [94] verified similar LS values in areas of low steepness. However, in the case of complex slopes, in more sloping areas, the LS values generated by the Desmet & Govers algorithm [46] were significantly superior. That can probably be explained by the assimilation of the convergence and by the respective flow accumulation of this method, which does not occur with the Wischmeier & Smith method [3].
Refining the method of Desmet & Govers, another study [80] incorporated an infinite flow direction model (D∞) and additional methods to isolate the slope length factor. Such method can be applied in situations where a complex topography can influence the surface runoff path and where the excessively long slope lengths, calculated from DEM, can be in need of new landscape detailing. The authors validated the method by the comparison of the statistical distribution of the LS values in GIS with the LS distribution, calculated from field observation data, providing support for applicability of the GIS method to obtain spatial heterogeneity and LS factor magnitude. The evaluation in GIS presented statistical distributions of the LS factor values very similar to those described in the field data, supplying strong support for the use of GIS based methods to represent the spatial heterogeneity and LS factor magnitude for the first time.
3.2.3. RUSLE–3D
From Equation 8, derived by Desmet & Govers, an LS factor equation was generated that is used by the RUSLE 3D model [9]. The model includes irregular hillsides integrating a wide spectrum of hillside convexities and concavities and it incorporates the contribution area A for the determination of the LS factor:
Gij=Gx2+Gy2E10
Where A(r) is the upstream contribution area (m2); β(r) is the slope inclination angle (degrees); and m and n are flow type dependent parameters.
Typical m values are 0.4-0.6 and for n 1-1.3. The exponents for the runoff and slope terms in the soil detachment and sediment transport equations reflect the interaction among different flow, detachment and soil transport types. Figure 4 shows the spatial pattern of the topographic potential by RUSLE-3D with different values for the exponent m. For laminar flow (m = 0.1), the detachment and transport of sediments increase relatively little with the amount of water. This type of flow is typical for areas with good plant covering, but also for a severely compacted soil, where the compacting prevents the detachment and formation of furrows. The value of the exponent m of this flow is low, represented by the contribution area [51].
In case the area presents both flow types, usually due to the spatial variability of soil use and properties, an m value=0.4 balances the impact of the surface laminar and turbulent flow and it supplies average satisfactory results [51] (Figure 4). When furrow and gully erosion in degraded soils vulnerable to the formation of deep furrows prevails, there are high water flow turbulence conditions and higher water impact, reflecting in a high exponent (m=0.6). The dense vegetation impedes the creation of furrows and maintains a dispersed water flow, while in situations of bare soil, the detachment caused by the flow turbulence increase leads to the formation of furrows [51].
Figure 4.
LS factor of RUSLE-3D with m value of = 0.1 for laminar flow; m = 0.4 for laminar and concentrated flow impact; and m = 0.6 for high flow impact in the erosion pattern. (Source: Elaborated by the authors).
The exponent of the spatial variable based on the covering can vary seeking to increase the negative impact of the disturbed areas and to reduce the impact in vegetated areas [51]. For instance, for forest m=0.2, for pasture m=0.4, for degraded pasture m=0.5 and for degraded areas m=0.6. In a study of these coefficient calibrations for two hydrographic sub-basins with forest vegetation, pastures and native field (prairie) in Mexico [95], the estimated value for m was 0.49. In the evaluation of the water erosion in forest systems [17] the value adopted for the coefficient was 0.4. In a hydrographic sub-basin with prevalence of native forest in Australia, the coefficient m=0.6 was considered more representative [96]. It was observed that the m value is low (m=0.1) when sediment detachment and transport increase relatively little with the amount of water. Thus, the geometric properties of the topography (slope, curvatures) play a more important role in the evolution of the soil detachment and erosion/deposition pattern than the water flow pattern [95].
The RUSLE model supplies an exponent m expressed in function of the slope angle that reflects the predominating dispersed flow in plane hillsides of gentle slope, while the flow in accentuated slopes is more turbulent. However, in the RUSLE-3D model this exponent supplies satisfactory results only for short segments. The formula for m based on the slope can result in values of 0.8 or higher in longer slopes. For slopes with hundreds of meters in length or for the concentrated flow this exponent predicts extremely high erosion rates in RUSLE-3D due to the contribution area [51]. As such, the RUSLE equation for the variable m was developed for the slope length and traditional field applications, and therefore it is not recommended for use as contribution area without evaluating the results through field measurements.
Considering this question, a study conducted the calibration of the m and n parameters through the comparison of the result of each soil loss estimated by the different coefficients, with the soil losses obtained in sample portions inserted in an analyzed sub-basin [48]. Joint analysis the values that presented good performance in relation to the mean error and mean difference for the soil loss estimates were determined. The obtained results, m=0.5 and n=1.0, correspond to those mentioned in [51] (m=0.4 and n=1.0) for areas with high spatial variability of use and soil properties under which both flow types, laminar and concentrated, occur. In fact, the forest use is predominant in the sub-basin and so the laminar flow is favored. At the same time, the high variability of soils and their respective properties also influenced by the relief can generate concentrated flows.
3.2.4. USPED
As the RUSLE-3D model, the Unit Stream Power Erosion and Deposition (USPED) [9] model is derived from USLE and represents its modifications or improvements. The model was developed considering the limitation of the empirical models when estimating the soil loss for convergent and divergent terrain in large areas allied to the Geographic |Information System (GIS). Proposing an adaptation of the contribution area variable the LS topographic factor was derived using the drainage force unit theory to describe the erosive process associated to the laminar and furrow flow in steep hillsides starting from a DEM [55-57].
An advantage of USPED is the fact that it predicts the spatial distribution of the erosion, as well as the deposition rates under conditions of uniform surface flow and high precipitation. Thus, this model can be applied in complex terrains where the erosion is limited by the capacity of the runoff to transport sediment. The topographic index represents the change in the transport capacity of the flow direction, being positive for areas with topographic potential for deposition and negative for areas with erosion potential. The contribution area is used as the representation of the water flow in a place or grid of cells. In USPED, the LS factor equation is [9]:
LS=(m+1)[A(r)22.13]m[sinβ(r)0.09]nE11
Where A is the contribution area (m2); θ is the slope angle; and m and n are constants that depend on the flow and soil property types. For situations where the furrow erosion dominates, these parameters are usually established as m = 1.6 and n = 1.3; where the laminar erosion prevails, m = n = 1.0 is considered [57, 97].
In this model, the water erosion in a DEM cell is dependent on the surface runoff in this cell that in turn depends on the upstream drainage area. When substituting the slope length the upstream contribution area generates the erosion network calculated as the convergence of the sediment flow and the deposition network obtained by the alteration in the sediment transport capacity.
Due to USPED computing divergence of the sediment flow, the impact of the exponents is more complex when compared to RUSLE 3D [51]. In USPED the water flow exponent controls the ratio between the erosion extension and deposition, reflecting the fact that the turbulent flow can transport sediments and the impact of the concentrated erosion will be wider than if the flow was dispersed throughout the vegetation. Figure 5 shows the spatial pattern of topographic potential by USPED with different values for the exponent m. For m = 1, a case of dispersed laminar flow and deposition along the hillside. With m = 1.4 we have the case of the influence of both flow types, laminar and in furrows, on the erosion and deposition, with the deposition beginning in the lower third of the hillside and gullying beginning in headwater areas. For m=1.6, furrows and concentrated flows prevail beginning with great force in the headwater areas and turning the erosion even longer and wider with potential for gullying. In this situation, the extension of the deposition areas is even more reduced.
Figure 5.
Spatial pattern of topographic potential for erosion and deposition by USPED with m=1.0; m=1.4; and m=1.6. (Source: Elaborated by the authors).
The variation of the LS factor coefficients of USPED interferes in the soil loss estimates by varying with the relief forms, plant covering and erosive processes. For this reason, the value of the exponents has been documented and established for different climates and areas. For the United States, the value suggested for n in [98] varies from 0.3 to 2.0. In laminar erosion situations the coefficient n=1.0 prevails and where the erosion in furrows is dominant, n=1.3. For a hydrographic basin of forest and agricultural use in Italy, the values adopted were m=n=1 [99], as well as in [95] after the calibration of these parameters. In the identification of the mining impact in an agricultural area of India, the coefficients m=1.6 and n=1.3 were adopted [100], while in Poland, they opted for m=1.4 and n=1.2 for two hydrographic basins with the presence of intense erosive processes [101]. In a sub-basin of forest use located in Brazil, the coefficients m=n=1.0 were determined [48].
As the conceptual models reflect the physical processes that govern the system describing them with empirical relationships, various quantitative evaluation studies of erosion risk in hydrographic basins have opted for the association of the USLE model with an LS factor that reflects the expected surface drainage according to the topography, in order to reach soil loss estimates closer to reality [1, 55, 56, 77, 79, 102-104]. The analysis of the erosion risk in a sub-basin was conducted evaluating the performance of four topographic factor models (USLE, RUSLE, RUSLE 3D and USPED) in the USLE model [48]. The USPED (0.1286 ton ha-1) and RUSLE 3D (0.0668 ton ha-1) models did not present statistical differences in relation to the field losses (0.1354 ton ha-1) and they generated a water erosion distribution meditated by the accumulated flow, while the LS factors of RUSLE (2.74 ton ha-1) and USLE (3.65 ton ha-1) overestimated the soil losses [48]. The model considered most efficient in the modeling of the erosion was USPED. This model represented the erosive process in a broad manner when estimating potential erosion and deposition areas, thus allowing to more precisely define the priority areas for conservationist practices under different management sceneries, agreeing with other studies [51, 99]. The advantages of USPED related to the possibility of predicting the spatial distribution of the erosion as well as the deposition rates were stood out in [105], after its comparison with the LS factor of RUSLE 3D. In this context, several research works are opting for the use of the USPED model [9, 45, 51, 98-100, 106, 107].
4. Models in real environmental scenarios
Application these topographic models in the real environmental scenarios have permitted more accuracy and faster estimate of soil erosion in different regions, reliefs and land uses than manual methods. This way, studies have tried to figure out better results applying different LS factor in the water erosion models to estimate and understand this process on watersheds.
LS RUSLE was utilized by [38] in the USLE model to estimate water erosion distribution caused by forest ecosystems in a small watershed and generating soil loss prediction maps according different land use situations. This same LS factor was used in USLE model by [108] allowing identification of the water erosion potential in a watershed forested with eucalyptus and by [109] to estimate of the sediment delivery ratio in a watershed upstream from the hydroelectricity plants.
[110] applied USPED to identify the influence of changing land use on erosion and sedimentation in different land use situations in watershed. [48] applied USLE, RUSLE 3D and USPED in a small watershed for predicting water erosion by eucalyptus plantation founding best results for USPED model. USPED and RUSLE were also applied for assessing the impact of soil erosion/deposition on the archaeological surface at the archaeological site in Greece and USPED presented better results [111]. Using USPED, [112] presented a modeling approach to implement the support practices factor using geographic systems information where data are unavailable and they concluded that USPED with adequate support practices permit to reduce erosion process.
Thus, considering the erosion problems in the world and data available efforts have been done to improve erosion models.
5. Final considerations
The analysis and obtaining of the topographic factors conducted in the digital environment has become a fundamental piece in erosion model progress, because they address the systematic analyses from specific Geographical Information Systems (GIS’s) tools, as well as allow the empirical processing of the data through adaptations of analogical techniques, thus maintaining researcher interpretation. The analysis of the topography in GI enables the analyses of the landscape on a large scale, considers the effects of the topographical complexity more fully in the soil erosion, makes the data processing easier and faster and reduces the relative cost. It is stood out that the reliability of these estimates is directly related to the precision of the topographic surveys used for the derivation of the digital elevation model (DEM).
Evolution of LS factor driven by advanced technology allows the application of different topographic models in the USLE/RUSLE equations modernizing, and improving the estimate of those models. In semi-empirical algorithms, where the contribution area constitutes the central concept, advantages include application in slopes of complex geometries, representation of the surface runoff paths and incorporation of the convergent and divergent flow impact by calibration of empirical coefficients that allow to indicate the qualitative and quantitative effects of the changes in the land use without demanding large spatial and temporal databases. Such models, besides determining the erosion areas on a hydrographic basin level, in the case of the USPED model, even allows to determine the deposition areas, including the erosive process to its full extent.
\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/41377.pdf",chapterXML:"https://mts.intechopen.com/source/xml/41377.xml",downloadPdfUrl:"/chapter/pdf-download/41377",previewPdfUrl:"/chapter/pdf-preview/41377",totalDownloads:3552,totalViews:517,totalCrossrefCites:8,totalDimensionsCites:24,totalAltmetricsMentions:0,impactScore:8,impactScorePercentile:97,impactScoreQuartile:4,hasAltmetrics:0,dateSubmitted:"April 24th 2012",dateReviewed:"October 23rd 2012",datePrePublished:null,datePublished:"February 27th 2013",dateFinished:"December 5th 2012",readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/41377",risUrl:"/chapter/ris/41377",book:{id:"3224",slug:"soil-processes-and-current-trends-in-quality-assessment"},signatures:"Anna Hoffmann Oliveira, Mayesse Aparecida da Silva, Marx Leandro Naves Silva, Nilton Curi, Gustavo Klinke Neto and Diego Antonio França de Freitas",authors:[{id:"155331",title:"M.Sc.",name:"Diego Antonio",middleName:null,surname:"Franca de Freitas",fullName:"Diego Antonio Franca de Freitas",slug:"diego-antonio-franca-de-freitas",email:"diego_ufla@yahoo.com.br",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Federal University of Lavras",institutionURL:null,country:{name:"Brazil"}}},{id:"156390",title:"Dr.",name:"Anna",middleName:null,surname:"Hoffmann Oliveira",fullName:"Anna Hoffmann Oliveira",slug:"anna-hoffmann-oliveira",email:"anna.ufla@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Federal University of Lavras",institutionURL:null,country:{name:"Brazil"}}},{id:"156395",title:"MSc.",name:"Mayesse",middleName:null,surname:"Aparecida Da Silva",fullName:"Mayesse Aparecida Da Silva",slug:"mayesse-aparecida-da-silva",email:"mayesse@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"156396",title:"Prof.",name:"Marx",middleName:null,surname:"Leandro Naves Silva",fullName:"Marx Leandro Naves Silva",slug:"marx-leandro-naves-silva",email:"marx@dcs.ufla.br",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"156397",title:"Prof.",name:"Nilton",middleName:null,surname:"Curi",fullName:"Nilton Curi",slug:"nilton-curi",email:"niltcuri@ufla.br",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"166453",title:"Mr.",name:"Gustavo",middleName:null,surname:"Klinke Neto",fullName:"Gustavo Klinke Neto",slug:"gustavo-klinke-neto",email:"gus@vitaramae.com.br",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Digital Elevation Model (DEM)",level:"1"},{id:"sec_3",title:"3. Water erosion modeling",level:"1"},{id:"sec_3_2",title:"3.1. USLE and RUSLE empirical modeling ",level:"2"},{id:"sec_3_3",title:"3.1.1. USLE and RUSLE limitations",level:"3"},{id:"sec_5_2",title:"3.2. Conceptual modeling",level:"2"},{id:"sec_5_3",title:"3.2.1. Contribution area modeling ",level:"3"},{id:"sec_6_3",title:"3.2.2. Desmet & Govers algorithm",level:"3"},{id:"sec_7_3",title:"3.2.3. RUSLE–3D",level:"3"},{id:"sec_8_3",title:"3.2.4. USPED",level:"3"},{id:"sec_11",title:"4. Models in real environmental scenarios",level:"1"},{id:"sec_12",title:"5. Final considerations",level:"1"}],chapterReferences:[{id:"B1",body:'Bloise, G.L.F.; Carvalho Júnior, O.A.; Reatto, A.; Guimarães, R.F.; Martins, E.S.; Carvalho, A.P.F. Avaliação da suscetibilidade natural à erosão dos solos da Bacia do Olaria – DF. Planaltina: Embrapa Cerrados, 2001. 33 p. 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Comment on “Length-slope factors for the Revised Universal Soil Loss Equation: simplified method of estimation”. Journal of Soil and Water Conservation. 1994. 49: 171–173.'},{id:"B98",body:'Pricope, N.G. Assessment of Spatial Patterns of Sediment Transport and Delivery for Soil and Water Conservation Programs. Journal of Spatial Hydrology, 2009. 9: (1)21-46.'},{id:"B99",body:'Pistocchi, A.; Cassani, G.; Zani, O. Use of the USPED model for mapping soil erosion and managing best land conservation practices. In: Rizzoli, A. E.; Jakeman, A. J. (eds.). Integrated Assessment and Decision Support, Proceedings of the First Biennial Meeting of the International Environmental Modelling and Software Society, v. 1, 2002. pp. 163-168. Disponível em: <iEMSs, 2002. http://www.iemss.org/iemss2002/proceedings/>.'},{id:"B100",body:'Kandrika, S.; Dwivedi, R.S. Assessment of the impact of mining on agricultural land using erosion-deposition model and space borne multispectral data. Journal of Spatial Hydrology, 2003. 3: (2).'},{id:"B101",body:'Drzewiecki, W.; Mularz, S. Model USPED jako narzędzie prognozowania efektów erozji i depozycji materiału glebowego. In: Annals of Geomatics (Associação Polonesa de Informação Espacial), 2005. 3: (2)52–54.'},{id:"B102",body:'Andrade, A.C.; Leal, L.R.; Carvalho Júnior, O.A.; Martins, E.S.; Reatto, A. Estudo dos processos erosivos na Bacia do Rio Grande (BA) como subsídio ao planejamento agroecológico. Planaltina, DF: Embrapa Cerrados, 2002. 26 p. Boletim técnico n. 63.'},{id:"B103",body:'Erdogan, E.H.; Erpul, G.; Bayramin, İ. Use of USLE/GIS Methodology for Predicting Soil Loss in a Semiarid Agricultural Watershed. Environmental Monitoring and Assessment, 2007. 131: 153–161.'},{id:"B104",body:'Jain, M.; Kothyari, U.C. Estimation of soil erosion and sediment yield using GIS. Hydrological Science Journal, 2000. 45: (5)771-786.'},{id:"B105",body:'Saavedra, C.P.; Mannaerts, C.M. Erosion estimation in an Andean catchment combining coarse and fine resolution satellite imagery. In: Proceedings of the 31st International Symposium on Remote Sensing of Environment: global monitoring for sustainability and security. Saint Petersburg, 20-24 June, 2005. 4 p.'},{id:"B106",body:'Liu, J.; Liu, S.; Tieszen, L.; Chen, M. Estimating soil erosion using the USPED model and consecutive remotely sensed land cover observations. Proceedings of the 2007 summer computer simulation conference, San Diego, 2007.'},{id:"B107",body:'Alimohammadi, A.; Sheshangosht, S.; Soltani, M.J. Evaluation of relations between DEM-Based USPED Model Output and Satellite-based spectral indices. In: Conference Proceedings of Map India 2006. Anais… Disponível em: <http://www.gisdevelopment.net/proceedings/mapindia/2006/index.htm>.'},{id:"B108",body:'Avanzi, J. C. Soil properties, condition and soil losses for south and east Brazilian forest areas. PhD thesis. Universidade Federal de Lavras, 2009.'},{id:"B109",body:'Beskow S.; Mello, C. R.; Norton, L. D.; Curi, N.; Viola, M. R.; Avanzi, J. C. Soil erosion prediction in the Grande River Basin, Brazil using distributed modeling. Catena, 2009. 79: 49-59.http://www.sciencedirect.com/science/article/B6VCG-4WKJ5JC-1/2/fb138c9c89ca86d834b52c3124987424 (accessed 19 October 2012). '},{id:"B110",body:'Leh, M.; Bajwa, S.; Chaubey, I. Impact of land use change on erosion risk: an integrated Remote sensing, geographic information system and modeling methodology. Land Degradation & Development, 2011. https://engineering.purdue.edu/ecohydrology/Pubs/2011%20LDD%20Leh-Bajwa-Chaubey.pdf. (accessed 19 October 2012). '},{id:"B111",body:'Gouma, M.; Wijngaarden, G. J. V.; Soetens, S. Assessing the effects of geomorp hological processe son archaeologic al densities: a GIS case stud y on Zakynth os Island, Greece. Journal of Archaeological Science, 2011. 38: 2714-2725'},{id:"B112",body:'Pelacani, S.; Märker, M.; Rodolfi, G. Simulation of soil erosion and deposition in a changing land use: A modelling approach to implement the support practice factor. Geomorphology, 2008. 99: 329 – 340'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Anna Hoffmann Oliveira",address:"anna.ufla@gmail.com",affiliation:'
Soil Science Department, Federal University of Lavras, Lavras, MG, Brazil
'},{corresp:null,contributorFullName:"Mayesse Aparecida da Silva",address:null,affiliation:'
Soil Science Department, Federal University of Lavras, Lavras, MG, Brazil
CAPES and CNPq (Visiting scholar) scholarships, Brazil
'},{corresp:null,contributorFullName:"Marx Leandro Naves Silva",address:null,affiliation:'
Soil Science Department, Federal University of Lavras, Lavras, MG, Brazil
Vitaramae Environmental Consulting Ltd., Lavras, MG, Brazil
'},{corresp:null,contributorFullName:"Diego Antonio França de Freitas",address:null,affiliation:'
Soil Science Department, Federal University of Lavras, Lavras, MG, Brazil
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1. Introduction
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The great journey of building rational scientific knowledge includes observing, making conjectures, and severely verifying them for flaws, limitations, and errors. When conjectures falter, scientists revisit, revise, abandon, start afresh, search for alternatives, etc. They seek unity in diversity or generalize to include diversity, with the knowledge that “truth” is not knowable. In this journey, they seek to be rational, parsimonious1 in making conjectures, and methodical, open, transparent, and consistent when sharing them. Conjectures are deemed scientifically valid only if there is potential scope of finding an error [1]. “Though [a mistake] stresses our fallibility it does not resign itself to scepticism, for it also stresses the fact that knowledge can grow, and that science can progress—just because we can learn from our mistakes” [1]. The process is criticism controlled.
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In the last few decades, technology has provided some remarkable tools to accelerate, not merely speed up, this process, and these tools have tremendous potential of becoming even more versatile. In the context of synthetic biology, the tools include the triad: clustered regularly interspaced short palindromic repeats (CRISPR) gene editing technology in genetic engineering, artificial intelligence (AI), and quantum computing (QC). There is also a torrential gathering of data since the Human Genome Project [2] published a draft sequence and initial analysis of the human genome in February 2001 [3]. The new sources include data flowing from the Human Cell Atlas project, which plans to identify and locate every type of cell we possess [4], and various brain projects initiated in the US, Europe, Japan, and Korea, and privately funded Allen Institute for Brain Science. China and Taiwan are also getting in the fray [5]. To make sense of the growing mountains of data in terms of finding “the molecular logic of the living state” in a timely manner rather than drowning in it will require data curation and analysis tools and resources that presently only CRISPR, AI, and QC can provide. This appears fortuitous since we anticipate a catastrophic speciation of the Homo sapiens to occur soon because of a rapidly changing environment that will likely lead to its decimation unless synthetic biology comes to the rescue.
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This chapter is therefore written for the millennials on whose shoulders will fall the responsibility of navigating through a socioeconomic epochal change that is already under way—the emerging postindustrial era—and a possibly unanticipated speciation of the Homo sapiens. The aim is to show that the time is ripe for synthetic biology, AI, and QC to join hands and form a purposeful, integrated discipline to further explore the secrets of life, create new life, and find harmonious ways by which the Homo sapiens can speciate in a controlled manner.
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2. Time for human speciation is near
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Biology is a game of creation, survival by adaptation, and annihilation; it is a game that is “red in tooth and claw”. Survival of the fittest (also called natural selection) means survival of those best able to adapt to the environment they are in. This is not about individual survival but of cohesive groups belonging to a species capable of exchanging genes or interbreeding. Natural selection is an ultraslow process in which sudden, dramatic changes in the environment generally mean sudden decimation of species living in it. Homo sapiens already find themselves in this unenviable but self-created situation that includes climate change (that also brings deadly heat, spreads diseases, overwhelms hospitals2), epidemics, automation initiated unemployment, large-scale immigration due to ism-related (e.g., political, religion related dogmatism) strife, concentration of information and wealth in the hands of fewer and fewer people, depletion of natural resources faster than its replenishment by Nature, the rising irrelevance of rote education, the escalating cost and deterioration of health care, a rising global population that embeds a disproportionately rising population from the less developed countries (see Figure 1), the rapidly rising population of the aged whose needs must be paid for by a shrinking, less fecund, younger working population (itself worried about an insecure and financially bleak future), etc. Each by itself is a major stress creator; collectively, they are approaching a crescendo portending an environmental catastrophe that leads to speciation or extinction, and destruction of the biosphere’s existing order.
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Figure 1.
(Left) World population growth. (Right) World population growth, 1950–2050. Source: World Population Prospects: The 2010 Revision, United Nations, 2011, http://www.un.org/en/development/desa/population/publications/pdf/trends/WPP2010/WPP2010_Volume-I_Comprehensive-Tables.pdf Note the rapidly increasing population size in the less developed countries.
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With the benefit of hindsight, we can discern the heralding signs of speciation that went unnoticed. In the rapidly growing global population (presently at 7.7 billion plus), the collective population of the more developed countries (characterized by high living standards and education, and low birth rate) since the last several decades has stabilized to about 1.3 billion (including immigrants), while that of the less developed countries (with opposite characteristics) is steadily rising. Concurrently, globally wealth has concentrated into fewer and fewer hands. In January 2018, Oxfam reported that “82% of all wealth created in the last year went to the top 1%, and nothing went to the bottom 50%”, that the wealthiest 42 people now had as much wealth as the poorest half, and two-thirds of billionaires wealth come from inheritance, monopoly, and cronyism [7, 8]. The environment for the poorest (hence unfittest) is already brutal.
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When natural speciation starts, its largest and earliest victims will come from the less developed countries before it hits the developed ones. In this respect, Africa appears to be highly vulnerable; it “has become the source of some of the greatest threats to the global economic order. Rather than capitalizing on opportunities, international engagement is increasingly focused on mitigating risks” [9]. When speciation begins, these risk mitigation efforts will be in vain because it is the global socioeconomic structure itself that will be disintegrating. The Homo sapiens’ incommensurate brain power will then make it vulnerable to extinction. The historical legacy of the Homo sapiens will not be its fossil record, but its amazing science, technology, engineering, and mathematics (STEM) record for successor species, if any, to peruse.
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Speciation is about adapting to the environment. Homo sapiens is the only known species to have developed substantial capacity to change the environment to its needs. Thus, it reduced the pressure for speciation since the agricultural era by adopting a socioeconomic structure built around division of labor and a tolerable taxation dogma of “from each according to his ability, to each according to his need” to temper Nature that is “red in tooth and claw”. That dogma is increasingly unsustainable because of an escalating need to subsidize the less well off. The affluent 1.3 billion can no longer subsidize the life span of the rest of the unemployable world. But there is the tantalizing possibility that since synthetic biology is ultrafast in editing DNA (deoxyribonucleic acid) and with advancing AI and QC, it will be even faster and better as compared to natural mutation and it may enable the Homo sapiens to initiate its own speciation in a programmed manner and survive extinction. What we cannot predict and may even fail to control once initiated are the unintended consequences that will certainly follow. If Ray Kurzweil’s prediction about the future capabilities of AI machines (“By 2029, computers will have human-level intelligence” [10]), turn out to be reasonably true, and genetic engineering continues at its present rate of development aided by advances in QC and in understanding RNA (ribonucleic acid)-mediated cellular activity using AI, artificially induced speciation of Homo sapiens by the end of this century may become possible before natural selection steps in anger.
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Kurzweil also forecasts that the future will provide opportunities of unparalleled human-machine synthesis:
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2029 is the consistent date I have predicted for when an AI will pass a valid Turing test and therefore achieve human levels of intelligence. I have set the date 2045 for the ‘Singularity’ which is when we will multiply our effective intelligence a billion-fold by merging with the intelligence we have created. [11]
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Kurzweil’s forecasts are based on his “law of accelerating returns” that enunciates that fundamental measures of information technology follow predictable and exponential trajectories seemingly unaffected by dramatic socioeconomic events such as war or peace, and prosperity or recession, paralleling Moore’s law in computer technology—the number of transistors on integrated circuit chips doubles approximately almost every 2 years. Indeed, it turns out that once a technology becomes de facto information technology, it comes under the grip of the law of accelerating returns because computer simulation of any technology is all about mathematics and computation. The exponential change is the inevitable effect of our ability to conceptualize in larger and larger conceptual blocks by aggregating and augmenting smaller conceptual blocks discovered earlier. This simple mechanism enables the human mind to deal with and find solutions to more complex problems by using the same number of but more versatile concepts rather than an unmanageably larger number of simpler concepts. The method is no different than what mathematicians do. We were first exposed to this method when we studied Euclidean geometry in school. Mathematicians start with simple, primitive concepts they call axioms and build more and more complex theorems as they go along. It works if the axiomatic system is consistent because once a theorem is proven, its validity can be taken for granted even by those who know nothing about mathematics, for example, by machines. This is how machines acquire “intelligence.”
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During the industrial era just behind us, most people reached their peak capacity to educate and skill themselves in activities (including earning a living) that required mechanizable “intelligent” rote education. That AI machines, in principle, can far surpass humans in such activities had become evident when Alan Turing showed how arithmetical calculations can be mechanized [12] and Gödel had earlier shown that any axiomatic system can be arithmetized [13]. This meant that any form of rational knowledge could be axiomatized and rote education programmed into computers. While creating new knowledge would still require human creativity, once that knowledge had matured and was formalized into an axiomatic system, it would be mechanizable and expandable. It would then be a matter of time that humans would increasingly face competition from machines and eventually be overwhelmed by them. Kurzweil’s prediction that this would happen during 2029–2045 is bolstered by recent advances in AI. Ongoing advances in deep learning by machines indicate that through self-learning they can become highly creative and creators of original technology (the patent system will go for a toss) and scientific discoveries without human intervention may well become the norm [14]. Of some 150 predictions since the 1990s, Kurzweil claims an 86% accuracy rate [11, 15, 16]. Since synthetic biology has now come under the grip of mathematics, its exponential development is certain. Synthetic biology is now a part of information technology.
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It is only in the last few years that the enormous significance of the exponential growth property of the law of accelerating returns has sunk in the minds of people. As one can see from Figure 2 (left), till one reaches the vicinity of the knee of the curve, the curve looks deceptively linear with a mild slope. This allows human minds to extrapolate into the future from gathered knowledge and experience. At the knee, the curve bends upward so rapidly that the human mind cannot respond fast enough to absorb, assess, contemplate, and react rationally. Knee-jerk reaction is about the best humans are capable of in such a situation. Homo sapiens now find themselves in an environment which they neither understand nor have the intellectual ability to rationally cope with. This is germane for triggering a speciation event.
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Figure 2.
Exponential growth. Source: (top left) Author. Nature of exponential growth. (Top right) Steve Jurvetson. An updated version of Moore’s Law (based on Kurzweil’s graph). Wikimedia Commons. https://commons.wikimedia.org/wiki/File:Moore%27s_Law_over_120_Years.png
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Once AI breaches a certain threshold, one should expect a runaway technological growth resulting in a phase transition in human civilization, including perhaps the speciation of the Homo sapiens. A likely component of the phase transition may well be that AI enters self-improvement cycles (feedback loops) that eventually cause it to evolve into a powerful level of superintelligence that would qualitatively surpass intelligence levels of all Homo sapiens. The accelerating progress of STEM in concert has also brought about commensurate changes in our lifestyle, expectations from life, and erosion of superstition and belief in religion. In the last few decades, the exponential nature of these changes has become noticeable and taxing enough even for the socioeconomic upper strata Homo sapiens to cope with. When, to mitigate their anxiety about AI, people claim that AI cannot do this or that which humans can, they often forget to ask if those tasks are worth doing.
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Exponential growth in AI has advanced the possibility that artificially induced speciation of Homo sapiens may occur by the end of this century. Recent findings show that Homo sapiens evolved about 300,000 years ago [17, 18].3 In recent times, their socioeconomic environment too has changed dramatically. Billions face the prospect of AI machines depriving them of sustainable livelihood and a dignified existence in society. Under such dramatic conditions of environmental change, Nature will force speciation toward life forms with an evolved brain far superior to that of the Homo sapiens. The very process may start too late and move too slowly and lead to the extinction of the Homo sapiens. Artificially induced speciation may therefore be the only means that may allow the Homo sapiens to transition to a new species in a controlled manner. On the flip side, one or more renegade group of Homo sapiens may strategize to surreptitiously create a colony of new species with the aim of dominating the Earth and decimating the Homo sapiens as an unnecessary burden on Earth.
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3. DNA is an information molecule
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The language of information now pervades molecular biology—genes are linear sequences of bases (like letters of an alphabet) that carry information (like words) to produce proteins (like sentences). For the process of going from DNA sequences to proteins, we use words like “transcription” and “translation,” and of passing genetic “information” from one generation to another. It is rather uncanny that molecular biology can be understood by ignoring chemistry and treating the DNA as a computer program (with enough input data included) in stored memory residing in a computer (the cellular machinery). It is this aspect that bioinformatics exploits. It is analogous to viewing Euclidean geometry not in terms of drawings but in terms of algebra.
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In a sense, in the DNA sequences in our cells, written using an alphabet of only four letters, lies hidden the story of who we are and where we come from. For all we know, it might even tell us where we might be going. Albert Lehninger wrote:
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… living organisms are composed of lifeless molecules … that conform to all the laws of chemistry but interact with each other in accordance with another set of principles—the molecular logic of the living state. [19]
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It is this “molecular logic of the living state” that is yet to be completely understood, and therein may lie our ability to understand emotion, cognition, and intelligence. So, in a deep sense, the DNA is the master molecule of life. A marvelous thing about cells is that they are so designed that for many purposes one can totally ignore their chemistry and think just about their logic. The fact that one can get away with this is one of the most elegant aspects of molecular biology. The algorithmic side of molecular biology is bioinformatics, the study of information flows in living matter. Bioinformatics is about the development and application of algorithms and methods to turn biological data into knowledge of biological systems. Of fundamental interest is the organization and control of genes in the DNA sequence, the identification of transcriptional units in the DNA, the prediction of protein structure from sequence, and the analysis of molecular function. If there is mathematical logic in living things, then one naturally seeks to determine the formal mathematical system that governs life, that is, how information in the DNA is stored and used by the rest of the cell’s machinery to do the myriad of things that it does.
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We already know that a DNA molecule—a genotype—is converted into a physical organism—a phenotype—by a very complex process, involving the manufacture of proteins, the replication of the DNA, the replication of cells, the gradual differentiation of cell types, and so on. This epigenetic process is guided by a set of enormously complex cycles of chemical reactions and feedback loops. By the time the full organism appears, there is no discernible similarity between the physical characteristics of the organism and its genotype. Yet molecular biologists attribute the physical structure of the organism to the information encoded in its DNA, and to that alone. This is because there is overwhelming experimental evidence that only DNA transmits hereditary properties. The genotype and the phenotype are isomorphic. However, this isomorphism is so complex that so far it has not been possible to divide the phenotype and genotype into parts, which can be mapped onto each other directly, unlike as, say, in the case of a music record and a record player where portions of a record’s track can be easily mapped to specific musical notes [20]. One hopes that AI and QC together will enable us to find this complex mapping. It is all about information processing.
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By information we mean the precise determination of sequence, either of bases in the nucleic acid or of amino acid residues in the protein. We gain knowledge of biological systems when we can interpret information in some “meaningful” way without it being easily refuted. That is, we make conjectures and put them through rigorous tests of refutations. Molecular biologists are becoming increasingly sure that “life is a partnership between genes and mathematics” [21]. Indeed, we increasingly tend to believe as Max Tegmark does about the Universe itself:
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Our reality isn’t just described by mathematics – it is mathematics … Not just aspects of it, but all of it, including you. [In other words,] our external physical reality is a mathematical structure. [22]
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One can well imagine the enormous strides synthetic biology will make when researchers get a deeper understanding of the Book of Life, with AI software becoming their research assistant, and quantum computers executing all the computing required by the AI software.
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4. The technology triad
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The time has come for synthetic biology, AI, and QC to join hands and form a purposeful, integrated discipline to further explore the secrets of life, create new life, and find harmonious ways by which Homo sapiens can speciate. The main responsibility will fall on the shoulders of the millennials. The technology triad (CRISPR, AI, and QC) share some important properties, the ability to create, share, process, and communicate information in digital form. This means they can be supported and integrated with the full power of mathematics and physics. As Richard Feynman notes:
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Mathematics is a language plus reasoning; it is like a language plus logic. Mathematics is a tool for reasoning. … [I]t is impossible to explain honestly the beauties of the laws of nature in a way that people can feel, without their having some deep understanding of mathematics. [23]
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Mathematics is the lingua franca of the physicists because a formal mathematical statement to be of any value is either true or false; it cannot be true to some and false to others. This is the reason why knowledge based on any axiomatic system, that is, a consistent system in which every valid statement or query has a “yes” or “no” answer, can be arithmetized (i.e., translated into arithmetical statements), encoded in a binary string, and processed in a digital computer. Mathematics is the language that binds men and machine together in a rational dialog. In short, axiomatic systems permit men and machines to mutually communicate without ambiguity or confusion. This is the foundation on which artificial intelligence (AI) rests. It is why Pierre Simon Marquis de Laplace did not even acknowledge God as the creator of the Universe in his mathematical magnum opus on celestial mechanics [24]. He famously told Napoleon Bonaparte, “I had no need of that hypothesis” [25].
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Creating and advancing rational knowledge, inter alia, requires an ability to communicate thoughts concisely, precisely, and accurately apart from refining knowledge by trial and error, that is, by making our conjectures fitter and fitter for survival. Benjamin Lee Whorf (1897–1941) said, “Language shapes the way we think, and determines what we can think about.” And Ludwig Wittgenstein (1889–1951) said, “The limits of my language mean the limits of my world.” Mathematics provides fewer limitations than any other language known to Homo sapiens. The power of mathematics lies in its ability to extract unity from diversity by abstraction, that is, by eliminating unnecessary context; it helps in discovering group properties (abstract or otherwise) common to all members of the group, for example, the DNA of a species.
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Both AI and QC are inseparable from mathematics; they are powerful means of processing and interpreting information (e.g., in the DNA) as well as aiding in inventing novel DNA for specific purposes. Both support and are supported by 3D printing that began by making plastic widgets, but now make guns, houses, prosthetic limbs, vehicle parts, etc. from inanimate matter. The day is not far off when it will advance to printing living, breathing, bio-organs, such as hearts [26] and kidneys, using nanotechnology, computer-aided engineering, and inanimate biodegradable or biocompatible materials and chemicals to build stem cells. Replicating and growing cells, say, in petri dishes is well established, and such cells are already in use as bioink in bioprinters. 3D printing offers the possibility of printing an entire organ, along with a system of arteries, capillaries, and veins that can support it [27, 28]. A major issue in developing this technology is to make it immune-system friendly, since the body may reject organs or cells thus produced, something that can occur even when tissue from one area of the body is put into another.
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4.1 CRISPR technology connects synthetic biology with information technology
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CRISPR technology has enabled a simple and affordable method of manipulating and editing DNA that has radically changed the ambitions of synthetic biologists. The technology promises to revolutionize how Homo sapiens may deal with the world’s biggest problems, for example, finding cures for cancer, blindness, and Alzheimer’s disease, improving food and eliminating food shortages, fulfilling organ transplant needs, and producing fuel and manufacturing chemicals. Biotechnologists are racing to develop the most efficient, precise, versatile, affordable, and commercially viable genome-editing tools possible. This will be a long and exciting race that may eventually lead to the Homo sapiens creating a super species that far exceeds them in the evolutionary path in a controlled manner.
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CRISPR is a series of short repeating DNA sequences with “spacers” separating them. The CRISPR technology harnesses an ancient bacteria-based defense system. Bacteria use these genetic sequences to “remember” the viruses that have attacked them by the simple mechanism of incorporating the virus’ DNA into their own bacterial genome. The viral DNA thus resides as spacers in the CRISPR sequence as identification tags the bacteria can use to mount an attack if the virus attacks again. Accompanying the CRISPR are locally stationed genes called Cas (Crispr-associated) genes. Once activated, these genes produce enzymes that act as “molecular scissors” that can cut into DNA with specificity. The significance is that in subsequent virus attacks, the bacteria can recall the virus signature and send RNA and Cas to locate and destroy the virus. Among the Cas enzymes derived from bacteria, Cas9 is the best-known molecular scissors enzyme for cutting animal and human DNA. Although the CRISPR sequence was first discovered in 1987, its function was discovered only in 2012.
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The ability to cut DNA allows one to either knock out, say, an unwanted disease-causing gene, or splice a “fixed” version of a gene into the DNA. This is analogous to the “Find & Replace” function in text editing software. Indeed, CRISPR technology has advanced so rapidly beyond the Find & Replace function that by December 2017, the Salk Institute had designed a version of the CRISPR-Cas9 system that could switch on or off a targeted gene without even editing the gene. The basic ingredients of gene editing are (1) a piece of RNA, called the guide RNA, that locates the targeted gene, (2) the scissors (the CRISPR-associated protein 9), and (3) the desired DNA segment for insertion after the break. Once the guide RNA locates the targeted gene, Cas9 makes a double-stranded break in the DNA carrying the targeted gene and replaces it with the desired DNA segment. A quick tutorial on CRISPR is available at [29].
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CRISPR-based therapies are still nascent. As expected, single-gene disorders are among the best understood because of their simple inheritance patterns (recessive or dominant) and relatively simple genetic etiology (cause). Such disorders include cystic fibrosis, hemochromatosis, Tay-Sachs, and sickle cell anemia. For example, cure for sickle cell disease (an inherited form of anemia in which distorted red blood cells—rigid, sticky, and shaped like sickles—are present in such numbers as to prevent adequate oxygen supply throughout the body) has gained prominence because it is related to an abnormal hemoglobin molecule, which comes from a well-understood genetic mutation. Hence efforts are concentrated on creating therapeutic strategies for fixing the mutated gene. Online Mendelian Inheritance in Man® (OMIM®) provides an Online Catalog of Human Genes and Genetic Disorders, a comprehensive database provides information about the etiology, clinical symptoms, and a bibliography of thousands of genetic conditions.4
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4.2 Artificial intelligence (AI)
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The true test of intelligence is not how much we know how to do, but how we behave when we don’t know what to do. [30]
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This behavior is a product of the brain-mind system an individual is born with and the environment it finds itself in. From conception to death, behavior and intelligence evolve through intimate interaction between the individual and the environment where the individual essentially tries to coexist with the environment by exploring networking strategies, inter alia, based on its information gathering and processing abilities (see Section 5.2). In the past few decades, in an ongoing process, the Homo sapiens using technology they have intelligently developed have already acquired massive amounts of information and placed it in easily accessible public repositories along with some sophisticated automated information processing services. This has happened unexpectedly, suddenly, and on a massive scale at an exponential rate in multiple disciplines (including molecular biology) due to breakthroughs in communication and computing technologies engineered by an exceptionally intelligent group of Homo sapiens. This development is well on its way to dwarfing the intellectual abilities of almost all Homo sapiens. In comparison, individual human brain capacity to understand, assimilate, create, and deal with knowledge appears pathetic and along with it, its ability to find gainful employment in the future. Machines are rapidly learning to create and deal with knowledge. On the positive side,
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There is a paradox in the growth of scientific knowledge. As information accumulates in ever more intimidating quantities, disconnected facts and impenetrable mysteries give way to rational explanations, and simplicity emerges from chaos. [31]
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It is this scientific knowledge ferreted out by a few geniuses among the Homo sapiens, which has allowed the species to extend their life span and improve their lifestyle by not just adapting to an environment but also by aiding the environment to adapt to humans. Along the way, Claude Shannon provided a mathematical theory that highlighted an important aspect of how data can be condensed and communicated efficiently in binary bitstreams. This was an important step in handling data by finding structure in data to reduce redundancy in data representation [32].
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Big data revolution, development and deployment of wearable medical devices, and mobile health applications have provided new powerful tools to the biomedical community for applying AI and machine learning algorithms to vast amount of data. Its impact in predictive analytics, precision medicine, virtual diagnosis, patient monitoring, and drug discovery and delivery is already being felt. More powerful advances are anticipated in the near future. Even at this early stage, AI excels even human experts in certain well but narrowly defined tasks. AI is at a stage where basic building blocks are being built. Soon we will learn to network these blocks and build increasingly powerful systems and subsystems that will solve increasingly complex problems and even create new knowledge. We already have a glimpse of it in Alphabet’s AlphaGo Zero’s ability to learn complex decision-making from scratch [33, 34]. “Previous versions of AlphaGo initially trained on thousands of human amateur and professional games to learn how to play Go. AlphaGo Zero skips this step and learns to play simply by playing games against itself, starting from completely random play. In doing so, it quickly surpassed human level of play and defeated the previously published champion-defeating version of AlphaGo by 100 games to 0” [34]. It acquired this ability within 40 days of self-training in an essentially iterative manner. The key here is the iterative strategy it used. Indeed, Homo sapiens too acquire knowledge iteratively but slowly over years and generations, collaboratively across space and time with other Homo sapiens, by making conjectures and refutations. It is rather uncanny that the essence of the process and its unusual power is mathematically captured by the Mandelbrot set in fractal geometry (see Section 5.3).
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Notwithstanding AlphaGo’s success, many real-life problems are still far too difficult not just for current AI systems but also for the vast-vast majority of Homo sapiens. The competition is really between two classes of geniuses: Homo sapiens who create ab initio knowledge and Homo sapiens who develop AI. Eventually, the latter is expected to win even if they must create an artificial brain using synthetic biology and place it in a humanoid! The task is enormously complex but not out-of-reach, in principle. What is needed is the ability to automate the task of observing and collecting data about the world and about us, create categories, data structures, and algorithms that would enable the collected data to be condensed into a computer program that can calculate the observations. This necessarily means that the size of the computer program (say, as represented by a binary string) must be as compact as possible (an index of the AI system’s intelligence) compared to the collected data (also represented by a binary string). Till this is accomplished, the collected data would remain incomprehensible, that is, algorithmically random, theory-less, unstructured, and irreducible [35]. This is what Homo sapiens in the genius class devote themselves to. As Oren Etzioni notes, machine learning is still 99% human work:
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The equation for AI success is to take a set of categories (for example, cats and dogs) and an enormous amount of data (that is labeled as to whether it is a cat or a dog), and then feed those two inputs through an algorithm. That produces the models that do the work for us. All three of those elements—categories, data, algorithm—are created through manual labor. [36]
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The solution to eliminating manual labor may well be the creation of an artificial brain using synthetic biology. For the present, AI serves mainly by “augmenting human intelligence”. But then automation too had begun by augmenting brawn (muscle) power to eventually become the superbrawn power during the industrial revolution. It only required the Homo sapiens to intelligently harness and control steam by first connecting water, heat, and work and then creating the thermodynamics, the science that would allow machines to make human brawn power look insignificant. Today’s augmented intelligence appears destined to become superintelligence. We have learnt to harness and control reasoning by first connecting logic, axiomatic systems and theorem proving. We are now advancing rapidly into understanding information theory so that quantum computers can become information engines to do intelligent work. It is interesting that the concept of entropy appears fundamental both in thermodynamics and information theory. Both are offsprings of rational thought in physics, and both are intimately related.5
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4.3 Quantum computing will power synthetic biology and AI
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Quantum mechanics deals with the world inhabited by photons, electrons, protons, atoms, molecules, etc. and how they interact among themselves to create larger matter entities. It is an incredibly mysterious world understood only in the language of advanced mathematics. This is the part of physics that tells us how atoms congregate into molecules by adjusting the electrons they carry into configurations that we call chemical bonds, how strong or weak those bonds will be or whether they will bond at all, what a congregation’s physical and chemical properties will be. It has led to many technical innovations and many more are expected, for example, in synthetic biology. The success of quantum mechanics in using mathematical abstractions is such that to a lay person it appears mystical, which even religious mystics cannot understand! Its remarkable success comes even though we still do not know what is meant by measurement in the quantum world and how the measurement process captures the information it outputs and why it releases information in a randomized way. Yet its success is undeniably visible:
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Quantum mechanics is an immensely successful theory. Not only have all its predictions been experimentally confirmed to an unprecedented level of accuracy, allowing for a detailed understanding of the atomic and subatomic aspects of matter; the theory also lies at the heart of many of the technological advances shaping modern society – not least the transistor and therefore all of the electronic equipment that surrounds us. [38, 39]
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Understanding quantum mechanics is out of reach except for a few thousand people in the world at any given time! This should immediately alert us to the fact that human intelligence needed to cope with AI-QC combination in the future will be very high and successor species of the Homo sapiens must evolve in the direction of better and smarter brains rather than any other physical trait. Computation, comprehension, and cognition are all a part of the brain’s activity, and we may assume that a sharper brain will come with a sharper mind. And we may further assume that comprehension and cognition are driven by computation in addition to using intuition, serendipity, flashes of inspiration, and inputs from the environment, etc. The keys are computation, problem-solving algorithms, and rational decision-making processes. These can be simulated by a classical computer, which itself has an abstract mathematical description we call the universal Turing machine (UTM) [12].
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Computing technology has now advanced to a stage where quantum computers can do everything that a UTM can do, and some more. A quantum computer’s phenomenal computing power comes from the extraordinary laws of quantum mechanics that include such esoteric concepts as superposition of quantum states, entanglement (“spooky action at a distance”), and tunneling through insulating walls, which, though highly counterintuitive, play extremely useful roles in understanding Nature at subatomic levels. However, it is not clear if these concepts can be ignored in biology and living processes in the way they are ignored in the design of cars and airplanes. May be not because there are areas in biology where quantum effects have been found, for example, in protein-pigment (or ligand) complex systems [40]. Thus, while the role of quantum mechanics is clear in quantum computing and hence in advancing both AI and synthetic biology research, it is not yet known if in the design of DNA, knowledge of quantum mechanics is required or that natural selection favors quantum-optimized processes. Essentially, we do not know if any cellular DNA maintains or can maintain sustained entangled quantum states between different parts of the DNA (even if it involves only atoms in a nucleotide). But we cannot rule out the possibility that sporadic random entanglements do occur that result in biological mutations or that researchers will not be able to achieve it in the laboratory and find novel uses for it in synthetic biology [41]. For example, in principle, it is possible to design molecular quantum computers, insert them in cells that can observe cellular activity, and activate select chemical pathways in the cell in a programmed manner. There is increasing speculation that some brain activity, for example, cognition, may be quantum mechanical [42].
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5. Integrating the triad: mechanization of speciation
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A combination of emerging technologies such as CRISPR, AI, and QC; new delivery models for products and services that form the core around which Homo sapiens organize themselves through collaborative division of labor; and talent migration, driven not by rote education but by innate creativity and global opportunities for employment open to them is disrupting and changing the character of the global talent pool that society needs today. Globalization has created opportunities for the talented to reach the skies, but in a resource-constrained world, it also means that many others must be or feel deprived. Sections 5.2 and 5.3 provide some glimpses of the dynamics of this situation captured in mathematics. Because mathematics is abstract, the depicted dynamics apply to entities and situations whether they are animate or inanimate. A resource-constrained world provides ample scope for adversarial dynamics in which some are predators and others are preys. Globalization has accentuated the problem at all levels of social structure, and since speciation is triggered by a changing environment, it affects the DNA. This has created survivability demands on the Homo sapiens. As this pressure mounts beyond endurance, Homo sapiens will face speciation by natural selection with uncertain outcomes. However, in the case of Homo sapiens, this process too may face a disruptive change because the highly intelligent among them may boldly initiate speciation using upcoming advances in synthetic biology, perhaps after perfecting their techniques by creating humanoids (a hybrid creation of life with embedded intelligent machinery). This will be a watershed event where a species takes on the task of speciation on itself. This remarkable possibility arises because Homo sapiens created and mastered mathematics, rational thought, computing machinery, and eventually deep data analytics so that life could be designed by them in the laboratory to create superior species.
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Synthetic biology, using methods and rational knowledge of molecular biology, physical sciences, and engineering, aims to design and construct novel biological parts, artificial biological pathways, devices, organisms, and systems for useful purposes. This will also permit us, at all levels of the hierarchy of biological structures (molecules, cells, tissues, and organisms), to redesign existing natural biological systems and may even help us recreate certain extinct species (if we can also recreate the environment, they had adapted to). It is not surprising that an extinct species has never revived itself since speciation and environment go together. Successes of synthetic biology will change the face of human civilization and almost certainly bring in new elements into play when Homo sapiens eventually speciate by playing an active role in it.
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Since the discovery of the double-helix structure of cellular DNA by James Watson and Francis Crick in 1953 [43] and its significance that the “precise sequence of the bases is the code which carries the genetical information …” (emphasis added) [44], the jargon and theory of information has invaded molecular biology (see Section 3). This enriched biotechnology and computational biology with nomenclature, definitions, concepts, and meanings. This also facilitates integration of synthetic biology with AI and QC. DNA is an information-carrying polymer. It is an organized chemical information database that inter alia carries the complete set of instructions for making all the proteins a cell will ever need.
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Just 20 years after Watson and Crick, in 1973 Cohen and Boyer published their pioneering work in recombinant DNA [45] and gave birth to genetic engineering and the biotechnology industry based on their patents [46] under liberal licensing terms. The next landmark was the creation of a bacterial cell controlled by a chemically synthesized genome by Craig Venter and his group in 2010 [47]. In 2014, Floyd Romesberg and colleagues [48] reported the creation of a semisynthetic organism with an expanded genetic alphabet by creating artificial nucleotides not found in Nature. Since its discovery in 2012 [49, 50, 51], CRISPR gene editing technology pioneered by Jennifer Doudna and Emmanuelle Charpentier, and Feng Zhang has come to occupy center stage in molecular biology as a new way of making precise, targeted changes to the genome of a cell or an organism. It has set the stage for major advances in synthetic biology (see Section 4.1). Another major advance was reported by Venter and his research group in March 2016 following their successful creation in 2010 of a bacterial cell controlled by a chemically synthesized genome noted above. In fact, they succeeded in creating a bacterium that contains the minimal genetic ingredients needed for free living. The genome of this bacterium consists of only 473 genes, including 149 whose precise biological function is unknown. It is a minimalist version of the genome of Mycoplasma mycoides [52, 53].
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Synthesis capabilities have developed at a pace where DNA synthesis is now automated. All one needs to do is to provide the desired DNA sequence to a vendor. Researchers in synthetic biology are now inching toward anticipating and preempting evolutionary events that if left to themselves would perhaps take a few million years to occur, and of even resurrecting extinct species. The time is ripe to integrate synthetic biology with AI and QC with a common language to enable seamless communication among them, connect with, and discover conceptual similarities for consistent integration of subsystems and validation of the whole system. That common language is mathematics; it comes with the added benefit that it can be used to also communicate between humans and machines. It is fortuitous that the DNA serves as the “Book of Life” that appears to have structure and grammar amenable to translation into mathematics. Once translated, biologists will discover some amazing patterns that have a direct bearing on life at the molecular level. We introduce a few of these below in brief.
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5.1 The molecular logic of the living state
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All macromolecules are constructed from a few simple compounds comprising a few atoms. It appears paradoxical that the DNA that serves as the epitome of life is itself lifeless. The molecule conforms to all the physical and chemical laws that describe the behavior of inanimate matter. All living organisms extract, transform, and use energy by interacting with the environment. Unlike inanimate matter, a living cell has the unique capacity, using the genetic information contained completely within itself, to grow and maintain itself and do mechanical, chemical, osmotic, and other types of work. But its most unique attribute is its programmed capacity to self-replicate and self-assemble. The great mystery that engulfs molecular biology is: “How does life emerge from an interacting collection of inanimate molecules that constitute living organisms to maintain and perpetuate life?” Once this is understood, chemical engineers will create a new life industry and commoditize it! Imagine buying customized pets as starters.
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As noted in Section 3, the mystery of life is almost certainly encoded in mathematics. The chemical basis of life is one indication because chemistry now has a strong mathematical foundation via quantum chemistry. Even more striking is the fact that all living organisms—bacterium, fish, plant, bird, animal—share common basic chemical features, for example, the same basic structural unit (the cell), the same kind of macromolecules (DNA, RNA (ribonucleic acids), and proteins) built from the same kind of monomeric subunits (nucleotides and amino acids), the same pathways for synthesis of cellular components, the same genetic code, and evolutionary ancestors. The monomeric subunits can be covalently linked in a virtually limitless variety of sequences just as the 26 letters of the English alphabet or the two binary numbers (0, 1) in binary arithmetic can be arranged into a limitless number of strings that stand for words, sentences, books, computer programs, etc.
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Organic compounds of molecular weight less than about 500, such as amino acids, nucleotides, and monosaccharides, serve as monomeric subunits of proteins, nucleic acids, and polysaccharides, respectively. A protein molecule may have a thousand or more amino acids linked in a chain, and DNA typically has millions of nucleotides arranged in sequence. Only a small number of chemical elements from the periodic table of chemistry appear in biomolecules. The carbon atom dominates and, by virtue of its special covalent bonding properties, permits the formation of a wide variety of molecules by bonding with itself, and atoms of hydrogen, oxygen, nitrogen, etc. Nature has placed further constraints. DNA is constructed from only four different kinds of subunits, the deoxyribonucleotides; the RNA is composed from just four types of ribonucleotides; and proteins are put together using 20 different kinds of amino acids. The 8 kinds of nucleotides (4 for DNA and 4 for RNA) from which all nucleic acids are built and the 20 amino acids from which all proteins are built are identical in all living organisms. So, at this level, living organisms are remarkably alike in their chemical makeup. This by itself provides a tantalizing hope that the DNA may indeed be completely decipherable as to its grammar and information content.
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The above observations strongly suggest the likelihood of an underlying, as yet undiscovered set of “axioms” of life that enforce emergent, organizing principles around which diverse life forms evolve and adapt to the environment at various levels, without transgressing any physical or chemical law. The organizing principles appear to include (1) Nature is red in tooth and claw (species are connected to each other in a predator-prey, food-chain relationship in a sparse resource matrix), (2) rules of genetic inheritance, (3) rules of environmental adaptation, and (4) rules of speciation. At each level, the rules are likely to appear stochastic given that there are innumerable interacting factors ranging from nature to nurture.
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5.2 Law of network phase transition
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In 1960, Erdős and Rényi [54, 55] proved a remarkable result in graph theory, which implies that when a large number of entities (e.g., men, machines, ideas, or arbitrary combinations of them represented by dots) begin to connect (link) randomly, a critical condition arises, following which a phase transition occurs in the way the entities form or reform into clusters of connected entities. The critical condition is reached when in a set of n dots, n/2 random links are made. The phase transition abruptly creates a giant connected component, while the next largest component is quite small. Such giant components then grow or shrink rather slowly with the number of dots as they continue to link or delink. Such behavior is observed in protein interaction networks, telephone call graphs, scientific collaboration graphs, and many others [56]. This immediately suggests an involuntary mechanism by which a society at various levels of evolution, by connections alone, spontaneously reorganizes itself as nodes (people, machines, resources, etc.) link or delink in apparent randomness. It is highly pronounced in an Internet of Things (IoT) connected world where the millennials spontaneously polarize on issue-based networks that concern them on social media.
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Synthetic biologists must never forget that between the molecular and environmental levels, there are multiple intermediate levels through which regulated command and control communications pass. At all levels, level-related phase transitions and predatory fights for resources can occur and spread to other levels. In fact, the intimately coupled relationship between Homo sapiens and the environment is often overlooked. We rarely note what Richard Ogle has that
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[I]n making sense of the world, acting intelligently, and solving problems creatively, we do not rely solely on our mind’s internal resources. Instead, we constantly have recourse to a vast array of culturally and socially embodied idea-spaces that populate the extended mind. These spaces … are rich with embedded intelligence that we have progressively offloaded into our physical, social, and cultural environment for the sake of simplifying the burden on our own minds of rendering the world intelligible. Sometimes the space of ideas thinks for us. [57]
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The deep significance of this intimate bonding between the Homo sapiens and the environment is that while they are adapting to the environment, they are also helping the environment to adapt to them. When entities connect, they also acquire emergent properties by virtue of the relationships they are bound by. Certain static group properties emerge based on the network’s topology, while dynamic properties emerge depending on the rate at which entities make, break, or modify connections. The fluctuating dynamics witnessed in the social media, for example, is common among the millennials.
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Rapidly increasing connectivity among men and machines has imposed upon the global socio-politico-economic structure, a series of issue-dependent phase transitions. More will occur in areas where massive connectivity is in the offing. Immediately before a transition, existing man-made laws begin to crack, and in the transition, they break down. Posttransition, new laws must be framed and enforced to establish order. Since such a phase transition is a statistical phenomenon, the only viable way of managing it is to manage groups by abbreviating individual rights. The emergence of strongman style of leadership and its contagious spreading across the world is thus to be expected because job-seeking millennials will expect them to destroy the past and create a new future over the rubble. It appears inevitable that many humans will perish during the transition for lack of jobs or their inability to adapt to new circumstances. Robots and humanoids will gain domination over main job clusters, while society undergoes radical structural changes. Ironically, robots neither need jobs, nor job satisfaction, nor a livelihood. There will be ruthlessness in the reorganization.
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5.3 The logistic map and the Mandelbrot set
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Consider the iteration xn + 1 = r xn (1 – xn), called the logistic map, and a number-pair (r, x0) where r > 0 and 0 < x0 < 1, and plot the points (r, xn → ∞). Note our interest is only in the long-term trajectory of x0 and not in its transitory phase. Note xn + (1 – xn) = 1. The plot (Figure 3) has numerous 2-pronged pitchforks and hence is called the bifurcation diagram. Depending on r, xn may be settled as for 0 < r ≤ 3, and beyond r = 3 migrating from one prong to another of available pitchforks for a given r in the bifurcation diagram. At r = 4 and beyond, migration is chaotic. In between r = 3.5 and 4, there is an intuitively unexpected white band where migration options are few. Such and other unexpected (not discussed here) display of rich complexity tethered to r independent of x0 (i.e., the starting state) caught researchers by great surprise.
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Figure 3.
The logistic map.
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There are countless situations for which the logistic map captures the essence of a situation. For example, in genetics it describes the change in gene frequency in time, or in epidemiology the fraction of the population infected at time t, or in economics it depicts the relationship between commodity quantity and price, or in theories of learning the number of bits of information one can remember after an interval, or in the propagation of rumors the number of people who have heard the rumor after time t, etc. The logistic map allows us to assess the volatility of an adversarial environment by assessing r, that is, the ferocity with which the predators and preys are battling for resources.
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Now consider the following complex iteration. Given the complex variable z = x + iy, where \n\ni\n=\n\n\n−\n1\n\n\n\n and the complex constant c = a + ib, pick a value for c, and iterate with the seed z0 = 0. If the iterations diverge, then c is not in the Mandelbrot set (it is in the escape set), otherwise (even when it is trapped in some repeating loop or is wandering chaotically), it is in the Mandelbrot set (black points in Figure 4) M. (Setting z0 equal to any point in the set that is not a periodic point gives the same result.) This is perhaps the most famous mathematical object yet known. It is a fractal object, an object that is irregular or fragmented at all scales. It is a major discovery of the late 20th century. It cannot be replicated in Euclidean geometry.
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Figure 4.
Mandelbrot set.
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In 1981–1982, Adrien Douady and John H. Hubbard [58] proved that the Mandelbrot set is connected. Quite astoundingly, the Mandelbrot set, when magnified enough, is seen to contain rough copies of itself, tiny bug-like objects (molecules) floating off from the main body, but no matter how great the magnification, none of these molecules exactly match any other (see Figure 5 and follow the white-bordered square from left to right). The boundary of M is where a Mandelbrot set computer program spends most of its time deciding if a point belongs there or not. The simplicity of the iterative formula and the complexity of the Mandelbrot set leave one wondering how such a simple formula can produce a shape of great organic beauty and infinite subtle variation.
\n
Figure 5.
Infinite variations of the Mandelbrot set are embedded in the set itself. Source: Ishaan Gulrajani, A zoom sequence of the Mandelbrot set showing quasi-self-similarity, 01 October 2011, https://commons.wikimedia.org/wiki/File:Blue_Mandelbrot_Zoom.jpg (Placed in public domain).
\n
Since the logistic map and the Mandelbrot set map quadratic functions, and both represent behavior under iteration, it is not surprising that a one-to-one correspondence exists between the constants r and c and that the bifurcations created by r correspond to features that come with changes in c along the real axis where the Mandelbrot set compresses the information in the bifurcation diagram, that is, the map shows the points where the map converges to periodic oscillations and its periodicity, while the Mandelbrot set marks all the points, which end up oscillating, but the periodicity information is encoded in the bulbs of the set (see Figure 6).
\n
Figure 6.
(Left) Connection between the logistic map and the Mandelbrot set. (Public domain) Source: Georg-Johann Lay, 07 April 2008, at https://commons.wikimedia.org/wiki/File:Verhulst-Mandelbrot-Bifurcation.jpg. (Right) Frank Klemm, Mandelbrot set with periodicity of limiting sequences. 12 August 2017. https://commons.wikimedia.org/wiki/File:Mandelbrot_Set_%E2%80%93_Periodicities_coloured.png licensed under the Creative Commons Attribution-Share Alike 3.0 Unported.
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It appears that the Mandelbrot set, inter alia, mimics the working of the mind. Its infinitely many variations embedded within itself seem to say that once the mind latches on to an idea and begins to deeply explore it, it does so by investigating its many variations, often in a random fashion (i.e., choosing c randomly), but does not abandon the core idea (the iterated function, equivalent of a law of Nature). On the other hand, if a mind randomly discovers a few of the dispersed similar looking sets, it begins a search for the mother set, M, itself. Is it then surprising that researchers often tackle new problems through random exploration based on a hunch (the iterated function), and if they are persistent enough, a solution finally emerges if the hunch is right? We see a game of conjectures and refutations at play here. On the other hand, the logistic map appears to work on a species scale where random interactions among minds lead to forming of societies (say, along the lines of the Erdős & Rényi theorem) functioning under constrained resources and an adversarial predator-prey law where the bifurcation points stand for points of speciation (measured in geological time scales).
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The pace at which a system is driven through cyclic (iterative, also called self-referential) processes, that is, cycles of construction and destruction constrained by recyclable finite resources, has a profound effect on how the system evolves. A remarkably simple model as the logistic map shows an amazing variety of nonintuitive dynamics that a nonlinear system can display. It too provides a basic involuntary mechanism by which a society spontaneously reorganizes itself. In his seminal paper on the logistic map, Robert May, a theoretical ecologist and former President of the Royal Society (2000–2005) was so struck by the deep relationship between complexity and stability in natural communities that he exhorted:
\n\n
Not only in research, but also in the everyday world of politics and economics, we would all be better off if more people realised that simple nonlinear systems do not necessarily possess simple dynamical properties. [59]
\n\n
What lessons can we draw from such simple mathematical models? For one, the logistic map indicates that the Earth’s supply chain (the environment) has been grossly disrupted. In this predator-prey game where some Homo sapiens turn into predators and the rest into preys, a massive capture of supplies by predators results in a massive population of preys, and the preys must mutate or speciate to survive or die. The logistic map decides how the selfish genes play the game while the Homo sapiens mainly decide the value of r. The Mandelbrot set tells us that while the laws of Nature need not change for the environment to change, it does contain enough complexities in the form of fractal structures whereby the environment may change enough to force speciation to take place in niches. In the present innovation-driven environment, speciation will push to enhance the brain-mind system of the Homo sapiens. In the process, synthetic biology may discover life as we do not know it. The survival of the fittest is a statistical law and hence it rests on an ensemble being available. The world’s current population certainly fulfills that.
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In the present global environment, saturated by connectivity between humans, machines, and ideas, the largest component emerging in any socioeconomic context is populated by the deprived who cannot fend for themselves. Inter alia, this is highly visible at multiple scales of population size (global, national, provincial, urban, etc.) and context (employment, access to health care, education, skill development, etc.). A wide spectrum of power, opportunities, and assets are grabbed by a minority by simply ignoring the plight of the desperate. This alone enforces a massive decimation of the Homo sapiens’ gene pool. Among the predators, many with inherited wealth (and hence generally lacking survival skills but not the means) too will become preys. In this planetary-scale debacle, a unique minority endowed with an exceptional brain-mind system, perhaps aided by AI and QC, will strive to improve their gene pool by artificial speciation6 using synthetic biology and insulate themselves in an artificially created environment to improve their cognitive abilities, life span, and fecundity. A look at the logistic map shows that as the new species advance even more rapidly, increasingly wild fluctuations in their fortunes will take place within their insulated, resource-constrained environment unless they reduce r by allowing the environment to replenish itself.
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In the absence of irreversible ecological damage, it is possible that, in the early stages, replenishment may happen by itself since Nature would have decimated a large component of the population from the less developed countries, thus presenting the survivors with a sudden increase in per capita resources. We may infer by analogy from the Mandelbrot set that once a new species survives long enough to avoid extinction (because it begins with a small population, which needs time to grow into adulthood), even if it is in some remote fringes of the set, it will likely someday reach the main (central) part of the set since the set is connected. Once this happens, the new species will likely continue for a very long time until it is decimated by the Sun entering its dying phase by turning into a giant red star. That will be a few billion years hence.
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\n
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5.4 Creating novel DNA algorithmically
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The way we acquire knowledge is iterative and nonlinear—we conjecture and put our conjectures on trial, that is, put them to severe critical tests (refutations). As the trial progresses, we edit, discard, refine, and add to our conjectures in a pseudorandom manner controlled by criticism, driven by instinct, hunches, inspiration, etc. Conjectures and refutations in scientific research are deemed self- and community-driven adversarial processes. We connect the dots. At every step of linking the dots, we consult the axioms (conjectures) and the rules for deriving conclusions (theorems) to ensure that we are within the axiomatic system we have put on trial. This means that the process leads us to understand the Universe solely based on our chosen beliefs (axiomatic system).
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As we learn from our mistakes our knowledge grows, even though we may never know—that is, know for certain. Since our knowledge can grow, there can be no reason here for despair of reason. And since we can never know for certain, there can be no authority here for any claim to authority, for conceit over our knowledge, or for smugness. [1, Preface]
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As far as we can tell, creating an axiomatic system is a nonmathematical and a highly intelligent act. Developing a sequence of theorems with a specific nontrivial goal in mind (developing algorithms) is also a highly intelligent act. However, executing an algorithm, once developed, can be mechanized and does not require intelligence, in fact, none at all. If the most useful aspect of intelligence is algorithmic, then it must be mechanizable and converted into computation. We believe the DNA is a book of knowledge about the birth and death of life. In principle, it is in machine-readable form. AI and quantum computing are the most powerful tools we presently have to decipher it. When AI drives our lives, it is the algorithm that really drives us.
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Some recent bold experiments using CRISPR gene editing have provided glimpses of DNA editing as a new source of creating a variety of biomatter and life forms. For example, experiments are in progress for producing meat (beef, pork, poultry, and sea food) without killing animals by growing meat in the laboratory from cultured stem cells by multiplying them dramatically and allowing them to differentiate into primitive fibers that then bulk up to form muscle tissue. This would substantially reduce environmental costs of meat production and eliminate much of the cruel and unethical treatment of animals [62]. Another example is producing offsprings from same-sex mice parents, again using stem cells and CRISPR gene editing technology [63].
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In another development, till recently it was believed that mitochondrion DNA (mtDNA) in nearly all mammals (including humans) is inherited exclusively from the mother. However, recently, Luo et al. [64] have uncovered multiple instances of biparental inheritance of mtDNA “spanning three unrelated multiple generation families, a result confirmed by independent sequencing across multiple unrelated laboratories with different methodologies. Surprisingly, this pattern of inheritance appears to be determined in an autosomal dominant like manner.” Given that the mitochondrion is an energy-producing organelle in the cell, this discovery will have profound implications in synthetic biology and in the design of new drugs.
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Once humans master the art of designing DNA for self-replicating, multicellular organisms (we already know how to design cells not found in Nature and edit DNA), they will create living species of their own design. We also anticipate that when AI machines master the art of learning from mistakes (i.e., the art of making conjectures and refuting them in a spiraling process toward better knowledge, a possibility that mathematically exists), they would have taught themselves how to handily beat humans in intelligent activities and thereby break the human monopoly on intelligence. The seeds of this were sown when the AI program called AlphaGo decisively defeated the world’s greatest Go players in 2016 [65, 66]. AlphaGo has achieved what many scientific researchers had dreamed of achieving. It means that a machine can teach itself in a tiny fraction of the time it takes humans to explore ab initio any axiomatic system. The last bastion of human supremacy over all other creatures on Earth in the form of intelligence has been cracked by AI machines. This is the world the millennials have stepped into. We have no idea how AI machines may organize themselves into networks and network with humans and vice versa. Will the future be written and created by humanoids with humans finding themselves relegated to footnotes and appendices once biotechnology and AI integrate? (See, e.g., [14].)
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So, what comes after Homo sapiens? Given the accelerating march of AI and computing, everything points to the dominating power of algorithms created and executed by quantum computers. It is a matter of understanding how to create novel DNA sequences and creating an environment for it to thrive. It is about writing lengthy books of life using natural and artificial nucleotides. With AI-embedded quantum computers capable of surpassing human intelligence, and the smartest among them developing Godlike abilities, the raw material they will be hunting for is massive amounts of data and mining that data for usable information for the welfare of one or more new species to whom the Homo sapiens will be ancestors.
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6. Conclusions
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The stage appears set for some remarkable advances in synthetic biology including artificial speciation as an alternative to the natural evolution of species. Homo sapiens are now poised to change the evolutionary destiny of life forms (including their own) they choose to target and even design-to-order new life forms. The ramifications are far and wide (see, e.g., [67]). Creating species that can thrive on other planets, colonizing the Moon with single-celled life, etc. are no longer science fiction fantasies.
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We, the Homo sapiens,7 have been around for about 300,000 years [17, 18]. Records of our civilization date back approximately 6000 years. Since Homo sapiens are still evolving, speciation may yet produce superior creatures with new attributes that can give them superior knowledge of the Universe and its origin. After all, it is speciation that made the Homo sapiens overwhelmingly superior in intellect from the great apes and our cousins, the chimpanzees with whom we share 96% of our DNA sequence. “Darwin wasn’t just provocative in saying that we descend from the apes—he didn’t go far enough. We are apes in every way, from our long arms and tailless bodies to our habits and temperament.”8 Yet, at an intellectual level, within a span of few centuries, at the knee of the exponential curve that breathed energetic intellectual life into our neural and socioeconomic networks, we have attained such remarkable feats as formalizing and mechanizing axiomatic systems, discovering deep secrets of the Universe, partially mechanizing brain-mind activities, developing technologies that augment, supplement, and amplify our comparatively puny brain and brawn capacities. Within the past century or so, we have fathomed the power and limitations of rational thought and binary arithmetic to express it in, mechanized arithmetical calculations to unimaginable heights, and used this mechanization to develop robotics, 3D precision manufacturing, biotechnology, AI, QC, cloud computing, etc. These developments are now rapidly networking, the scale of which is such that we now see the combined effects of phase transition of graph theory in the Internet of Things (IoT) (creation and destruction of interlinked man-machine-idea components), of the logistic map in the rapidly changing socioeconomic scenarios that have increasingly made predicting the future at all levels of aggregating individuals a game of dice. The relationship between the logistic map and the Mandelbrot set implies that the future of Homo sapiens will indeed be so complex that a new species capable of handling that level of complexity must either evolve or be artificially created.
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The raw physical limitations of the Homo sapiens’ brain-mind system is distressingly visible in its waning ability to earn a living. Barring exceptional Homo sapiens, our search for meaning in life is now propelled by search engines roaming the Internet and not by our brains. The World Wide Web (WWW) has changed the way we think, what we think about, and how we communicate our thoughts. The millennials’ cognitive abilities are very different from those they were born with and weaned on before the Internet invaded their lives. They are shaped not just by what they read but by how they read. Not only has their lifestyle changed but also has their thought style. All the work of the mind—deep thinking, exhaustive reading, deep analysis, introspection, etc.—is now delegated to AI machines. Humans have thus relinquished their right to control their individual lives and direct their souls (maybe deep inside they already know there is no soul!). If machines can outdo humans so easily without a soul, then perhaps the soul is holding humans back from reaching their potential. Perhaps it is time, AI machines became our role models and our mentors [14].
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Modern computers have made increasingly powerful and compute intensive mathematical algorithms accessible to even those not trained in science and mathematics for solving complex problems. Rapid advances in artificial intelligence (AI) and quantum computing show an inevitable trend that a vast array of human activities that till now required intelligent Homo sapiens to perform and earn a livelihood will soon be performed by AI-enabled computers, including the design of cellular life forms. When this happens, can human-designed speciation of life forms, its DNA coded for superintelligence, and other designed characteristics be prevented by the Homo sapiens’ instinct for survival? One day, nanotechnology will enable biocompatible, implantable, programmable quantum computers to be embedded into our organs or even introduce specialized new miniature organs, and we will be on our way to creating humanoids. We do not know how this will affect the speciation of the Homo sapiens. But before insight-driven complex experimentation aided by deep computing can happen; AI, new quantum algorithms, and embeddable quantum computers will have to evolve. Some early successes, for example, creation of artificial nucleotides, designed cells, attempts at resurrecting extinct species, etc. in molecular biology, indicate that once we master the biochemistry of very-very large molecules, for example, the DNA, RNA, proteins, by understanding their structure and their chemical-structural dynamics through quantum mechanical models, interactions between living and nonliving matter will undergo a sea change.
\n
We therefore anticipate a forced speciation of the Homo sapiens. It will drastically reduce the emergence time for a new species to a few years compared to Nature’s hundreds of millennia. Accelerated speciation by Homo sapiens via domestication, gene splicing, and gene drive mechanisms is now scientifically well understood. Synthetic biology can advance speciation far more rapidly using a combination of CRISPR technology, advanced computing technologies, and knowledge creation using AI. There is no reason why Homo sapiens themselves will not initiate their own speciation once synthetic biology advances to a level where it can safely modify the brain to temper emotion and enhance rational thinking as a means of competing against AI-embedded machines guided by quantum algorithms.
\n
Rapidly advancing research in the life sciences, while promising tools to meet global challenges in health, agriculture, the environment, and economic development, some of which are already on the horizon, also raises the specter of new social, ethical, legal, and security challenges. These include the development of ethical principles for human genome editing, establishment of regulatory systems for the safe conduct of field trials of gene drive-modified organisms, and many others. Additional concerns arise since the knowledge, tools, and techniques resulting from such research could easily lead to the development of bioweapons, facilitate bioterrorism, and the extinction of the Homo sapiens themselves. All these concerns are global not merely national [69]. The subject of this chapter goes beyond such concerns because here the concern is the possibility of self-initiated speciation of the Homo sapiens. The ramification of such a self-referential (iterative) process akin to that of the logistic map and the Mandelbrot set involving, in addition, phase transitions seen in graph theory is unknown. The perspective presented in this chapter is vastly different from that of Erwin Schrödinger (among the pioneers of quantum mechanics) expressed in 1944 [70]. Much water has flown under the bridge since then. A decade later, in 1953, when the structure of the DNA and its role in replicating life was discovered by Watson and Crick [43, 44], molecular biology was born. That led to genetic engineering [45] and synthetic biology [47]. As we write, CRISPR-Cas9 has been used to alter the embryonic genes of twin girls born in December 2018 in China [60, 61], which has elicited deep concern in the scientific community and an immediate response from the WHO: “Gene editing may have unintended consequences, this is uncharted water and it has to be taken seriously … WHO is putting together experts. We will work with member states to do everything we can to make sure of all issues—be it ethical, social, safety—before any manipulation is done” [71]. On the heels of this report comes the news that the world’s first baby born via womb transplant from a dead donor has been successfully achieved in Brazil [72]. With CRISPR, AI, and QC, the Homo sapiens are now on the threshold of creating new life forms and initiating even their own speciation.
\n
\n\n',keywords:"synthetic biology, artificial intelligence, quantum computing, information theory, genetic engineering",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/65149.pdf",chapterXML:"https://mts.intechopen.com/source/xml/65149.xml",downloadPdfUrl:"/chapter/pdf-download/65149",previewPdfUrl:"/chapter/pdf-preview/65149",totalDownloads:2103,totalViews:0,totalCrossrefCites:1,dateSubmitted:"October 2nd 2018",dateReviewed:"December 10th 2018",datePrePublished:"January 13th 2019",datePublished:"February 12th 2020",dateFinished:"January 10th 2019",readingETA:"0",abstract:"We envisage a world where genetic engineering, artificial intelligence (AI), and quantum computing (QC) will coalesce to bring about a forced speciation of the Homo sapiens. A forced speciation will drastically reduce the emergence time for a new species to a few years compared to Nature’s hundreds of millennia. In this chapter, we explain the basic concepts that would allow a forced speciation of the Homo sapiens to occur and its consequences on life on Earth thereafter. Accelerating speciation mediated by Homo sapiens via domestication, gene splicing, and gene drive mechanisms is now scientifically well understood. Synthetic biology can advance speciation far more rapidly using a combination of clustered regularly interspaced short palindromic repeats (CRISPR) technology, advanced computing technologies, and knowledge creation using AI. The day is perhaps not far off when Homo sapiens itself will initiate its own speciation once it advances synthetic biology to a level where it can safely modify the brain to temper emotion and enhance rational thinking as a means of competing against AI-embedded machines guided by quantum algorithms.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/65149",risUrl:"/chapter/ris/65149",signatures:"Rajendra K. Bera",book:{id:"7728",type:"book",title:"Synthetic Biology",subtitle:"New Interdisciplinary Science",fullTitle:"Synthetic Biology - New Interdisciplinary Science",slug:"synthetic-biology-new-interdisciplinary-science",publishedDate:"February 12th 2020",bookSignature:"Madan L. Nagpal, Oana-Maria Boldura, Cornel Baltă and Shymaa Enany",coverURL:"https://cdn.intechopen.com/books/images_new/7728.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-78984-090-2",printIsbn:"978-1-78984-089-6",pdfIsbn:"978-1-78985-329-2",isAvailableForWebshopOrdering:!0,editors:[{id:"182681",title:"Dr.",name:"Madan L.",middleName:null,surname:"Nagpal",slug:"madan-l.-nagpal",fullName:"Madan L. Nagpal"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"77013",title:"Prof.",name:"Rajendra",middleName:null,surname:"Bera",fullName:"Rajendra Bera",slug:"rajendra-bera",email:"rajendrabera@yahoo.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"International Institute of Information Technology",institutionURL:null,country:{name:"India"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Time for human speciation is near",level:"1"},{id:"sec_3",title:"3. DNA is an information molecule",level:"1"},{id:"sec_4",title:"4. The technology triad",level:"1"},{id:"sec_4_2",title:"4.1 CRISPR technology connects synthetic biology with information technology",level:"2"},{id:"sec_5_2",title:"4.2 Artificial intelligence (AI)",level:"2"},{id:"sec_6_2",title:"4.3 Quantum computing will power synthetic biology and AI",level:"2"},{id:"sec_8",title:"5. Integrating the triad: mechanization of speciation",level:"1"},{id:"sec_8_2",title:"5.1 The molecular logic of the living state",level:"2"},{id:"sec_9_2",title:"5.2 Law of network phase transition",level:"2"},{id:"sec_10_2",title:"5.3 The logistic map and the Mandelbrot set",level:"2"},{id:"sec_11_2",title:"5.4 Creating novel DNA algorithmically",level:"2"},{id:"sec_13",title:"6. Conclusions",level:"1"}],chapterReferences:[{id:"B1",body:'Popper KR. Conjectures and Refutations: The Growth of Scientific Knowledge. London: Routledge; 1963. 412 p\n'},{id:"B2",body:'An Overview of the Human Genome Project. National Human Genome Research Institute. No date. 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London: Macmillan; 1893. p. 423. Available from: https://archive.org/details/117770582 [Accessed: 01-12-2018]\n'},{id:"B26",body:'O’Neill S. How to 3D-print a living, beating heart. New Scientist. 2018. Available from: https://www.newscientist.com/article/mg24032040-400-how-to-3d-print-a-living-beating-heart/ [Accessed: 01-12-2018]\n'},{id:"B27",body:'Gilpin L. 3D ‘bioprinting’: 10 things you should know about how it works. TechRepublic. 2014. Available from: https://www.techrepublic.com/article/3d-bioprinting-10-things-you-should-know-about-how-it-works/ [Accessed: 01-12-2018]\n'},{id:"B28",body:'Bioprinted Human Tissue. Organo. 2018. Available from: https://organovo.com/science-technology/bioprinting-process/ [Accessed: 01-12-2018]\n'},{id:"B29",body:'Synthego. Everything You Need to Know About CRISPR-Cas9. CRISPER 101. 2018. Available from: https://www.synthego.com/learn/crispr [Accessed: 02-12-2018]\n'},{id:"B30",body:'Holt JC. How Children fail. Penguin Education; 1964, p. 163. 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One Hundred Years of the Bohr Atom: Proceedings from a Conference. Scientia Danica. Series M: Mathematica et Physica. Vol. 1. Copenhagen: Royal Danish Academy of Sciences and Letters; 2015. pp. 419-434. Available from: http://philsci-archive.pitt.edu/11785/1/BohrQuantumWorld.pdf [Accessed: 02-12-2018]\n'},{id:"B39",body:'Zinkernagel H. Niels Bohr on the wave function and the classical/quantum divide. Studies in History and Philosophy of Modern Physics. 2016;53:9-19. Available from: https://arxiv.org/abs/1603.00353 [Accessed: 02-12-2018]\n'},{id:"B40",body:'Brookes JC. Quantum effects in biology: Golden rule in enzymes, olfaction, photosynthesis and magnetodetection. Proceedings of the Royal Society A, Mathematical, Physical and Engineering Sciences. 2017;473:20160822. DOI: 10.1098/rspa.2016.0822\n'},{id:"B41",body:'Brooks M. Is quantum physics behind your brain’s ability to think? New Scientist, 2015. 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Proceedings of the National Academy of Sciences of the United States of America. 1973;70(11):3240-3244. DOI: 10.1073/pnas.70.11.3240\n'},{id:"B46",body:'Bera RK. The story of the Cohen-Boyer patents. Current Science. 2009;96(6):760-763. Available from: http://www.currentscience.ac.in/Downloads/article_id_096_06_0760_0763_0.pdf [Accessed: 02-12-2018]\n'},{id:"B47",body:'Gibson D et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science. 2010;329(5987):52-56. DOI: 10.1126/science.1190719\n'},{id:"B48",body:'Malyshev DA et al. A semi-synthetic organism with an expanded genetic alphabet. Nature. 2014;509(7500):385-388. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24805238 [Accessed: 02-12-2018]\n'},{id:"B49",body:'Jinek M et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816-821. Available from: https://www.ncbi.nlm.nih.gov/pubmed/22745249 [Accessed: 02-12-2018]\n'},{id:"B50",body:'Cong L et al. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013;339(6121):819-823. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795411/ [Accessed: 02-12-2018]\n'},{id:"B51",body:'Sharlach M. CRISPR Pioneers Honored. The Scientist. 2014. Available from: http://www.the-scientist.com/?articles.view/articleNo/41455/title/CRISPR-Pioneers-Honored/ [Accessed: 02-12-2018]\n'},{id:"B52",body:'Hutchison III CA et al. Design and synthesis of a minimal bacterial genome. Science. 2016;351(6280):1414, aad6253-1-11. DOI: 10.1126/science.aad6253\n'},{id:"B53",body:'Saey TH. Scientists build minimum-genome bacterium. Science News. 2016. Available from: https://www.sciencenews.org/article/scientists-build-minimum-genome-bacterium [Accessed: 02-12-2018]\n'},{id:"B54",body:'Erdös P, Rényi A. On the evolution of random graphs. Publications of the Hungarian Academy of Sciences. 1960;5(1):17-61. Available from: https://users.renyi.hu/~p_erdos/1960-10.pdf [Accessed: 02-12-2018]\n'},{id:"B55",body:'Krivelevich M, Sudakov B. The Phase Transition in Random Graphs—A Simple Proof. arXiv: 1201.6529v4 [math.CO]. 2012. Available from: https://arxiv.org/pdf/1201.6529v4.pdf [Accessed: 02-12-2018]\n'},{id:"B56",body:'Bollobas B, Janson S, Riordan O. The Phase Transition in Inhomogeneous Random Graphs. arXiv:math/0504589 [math.PR]. 2007. Available from: https://arxiv.org/abs/math/0504589 [Accessed: 02-12-2018]. Also as: Random Structures and Algorithms. 2007;31:3-122\n'},{id:"B57",body:'Ogle R. Smart World: Breakthrough Creativity and the New Science of Ideas. Boston, Massachusetts: Harvard Business School Press; 2007. 303 p\n'},{id:"B58",body:'Douady A, Hubbard JH. Exploring the Mandelbrot set. The Orsay Notes. 2009. Available from: http://pi.math.cornell.edu/~hubbard/OrsayEnglish.pdf [Accessed: 03-12-2018]\n'},{id:"B59",body:'May RM. Simple mathematical models with very complicated dynamics. Nature. 1976;261:459-467. Available from: http://abel.harvard.edu/archive/118r_spring_05/docs/may.pdf [Accessed: 03-12-2018]\n'},{id:"B60",body:'Wilson C. Scientists are now very sure that the babies really were gene-edited. New Scientist. 2018. Available from: https://www.newscientist.com/article/2186956-scientists-are-now-very-sure-that-the-babies-really-were-gene-edited/ [Accessed: 03-12-2018]\n'},{id:"B61",body:'Le Page M. CRISPR Babies: More Details on the Experiment that Shocked the World. New Scientist. 2018. Available from: https://www.newscientist.com/article/2186911-crispr-babies-more-details-on-the-experiment-that-shocked-the-world/ [Accessed: 03-12-2018]\n'},{id:"B62",body:'Schaefer G. Lab-Grown Meat. Scientific American. 2018. Available from: https://www.scientificamerican.com/article/lab-grown-meat/ [Accessed: 03-12-2018]\n'},{id:"B63",body:'Maron DF. Same-sex mice parents give birth to healthy brood. Scientific American. 2018. Available from: https://www.scientificamerican.com/article/same-sex-mice-parents-give-birth-to-healthy-brood/ [Accessed: 03-12-2018]\n'},{id:"B64",body:'Luo S et al. Biparental inheritance of mitochondrial DNA in humans. In: Proceedings of the National Academy of Sciences. 2018. DOI: 10.1073/pnas.1810946115\n'},{id:"B65",body:'Gibney E. Google masters go. Nature. 2016;529:445-446. Available from: http://www.nature.com/polopoly_fs/1.19234!/menu/main/topColumns/topLeftColumn/pdf/529445a.pdf [Accessed: 03-12-2018]\n'},{id:"B66",body:'Gibney E. Google secretly tested AI bot. Nature. 2017;541:142. Available from: http://www.nature.com/polopoly_fs/1.21253!/menu/main/topColumns/topLeftColumn/pdf/nature.2017.21253.pdf [Accessed: 03-12-2018]\n'},{id:"B67",body:'Doudna JA, Sternberg SHA. Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution. New York: Houghton Mifflin Harcourt; 2017. 307 p\n'},{id:"B68",body:'Lovgren S. Chimps, Humans 96 Percent the Same, Gene Study Finds. National Geographic News. 2005. Available from: https://news.nationalgeographic.com/news/2005/08/0831_050831_chimp_genes.html [Accessed: 03-12-2018]\n'},{id:"B69",body:'Revill J, Husbands J, Bowman K, Rapporteurs. Governance of Dual Use Research in the Life Sciences: Advancing Global Consensus on Research Oversight: Proceedings of a Workshop. Washington, DC: The National Academies Press; 2018. Available from: http://nap.edu/25154 [Accessed: 04-12-2018]\n'},{id:"B70",body:'Schrödinger E. What is life? The physical aspect of the living Cell. 1944. Available from: http://www.whatislife.ie/downloads/What-is-Life.pdf [Accessed: 04-12-2018]\n'},{id:"B71",body:'Nebehay S. WHO Looks at Standards in ‘Uncharted Water’ of Gene Editing. Reuters. 2018. Available from: https://www.reuters.com/article/us-china-health-who/who-looks-at-standards-in-uncharted-water-of-gene-editing-idUSKBN1O227Q [Accessed: 05-12-2018]\n'},{id:"B72",body:'Kelland K. World’s First Baby Born via Womb Transplant from Dead Donor. Reuters. 2018. Available from: https://www.reuters.com/article/us-health-womb-transplant/worlds-first-baby-born-via-womb-transplant-from-dead-donor-idUSKBN1O32WS [Accessed: 05-12-2018]\n'}],footnotes:[{id:"fn1",explanation:'Following William Occam (1287–1347) who recommended a principle of parsimony (les parsimoniae), famously known as Occam’s razor, "plurality is not to be posited without necessity".'},{id:"fn2",explanation:"Its consequences on human health was recently highlighted in [6]."},{id:"fn3",explanation:"Prior to these papers, Homo sapiens were said to have been around for about 200,000 years."},{id:"fn4",explanation:'"OMIM contain information on all known mendelian disorders and over 15,000 genes. OMIM focuses on the relationship between phenotype and genotype. It is updated daily, and the entries contain copious links to other genetics resources." http://omim.org/about'},{id:"fn5",explanation:"This we know from the explanation of the Maxwell’s demon paradox in thermodynamics. See, e.g., [37]."},{id:"fn6",explanation:"A controversial experiment to this effect seems to have been successfully conducted by He Jiankui who recently presented his work at the Second International Summit on Human Genome Editing in Hong Kong, November 27–29, 2018, http://www.nationalacademies.org/gene-editing/2nd_summit/index.htm [60, 61]."},{id:"fn7",explanation:"The term Homo sapiens was coined by Carl Linnaeus in 1758."},{id:"fn8",explanation:"A quote from Frans de Waal, a primate scientist at Emory University in Atlanta, Georgia, as it appeared in [68]."}],contributors:[{corresp:"yes",contributorFullName:"Rajendra K. Bera",address:"rajendrabera@yahoo.com",affiliation:'
Acadinnet Education Services India Pvt. Ltd., Bangalore, India
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IntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
",metaTitle:"Retraction and Correction Policy",metaDescription:"Retraction and Correction Policy",metaKeywords:null,canonicalURL:"/page/retraction-and-correction-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"
IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\\n\\n
1. RETRACTIONS
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A Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\\n\\n
A formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
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Data fabrication
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Data recycling in a purportedly original research article
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Severe plagiarism - whether or not the plagiarism is to be deemed severe will be determined by the Academic Editor and verified by plagiarism checking software
\\n\\t
Double publication
\\n\\t
Copyright infringement - for example, if a Chapter uses copyrighted figures without permission.
\\n\\t
Unreliable findings
\\n\\t
Unethical research practices
\\n\\t
Any other practice or act considered potentially harmful to the scientific community.
\\n
\\n\\n
Publishing of a Retraction Notice will adhere to the following guidelines:
\\n\\n\\n\\t
All relevant bibliographic information about a retracted Chapter will be given in the title.
\\n\\t
A Retraction Notice will be published as a regular book Chapter and will be given its own Chapter number.
\\n\\n\\n
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Authors shall be required to approve a proposed retraction of their Chapter. If Authors maintain that their Chapter should not be retracted, the Academic Editor may issue a Statement of Concern (see 2. below).
\\n
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1.2. REMOVALS AND CANCELLATIONS
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Additionally, a Chapter retracted on grounds of copyright infringement (e.g. double publication) may be Removed by the publisher should the original copyright owner request such action. A Chapter retracted on grounds of its potential to harm the scientific community, for example, when a Chapter is defamatory in nature, may also be subject to removal.
\\n\\t
No formal Removal Notice will be published but a notice citing the reason for removal will be prominently displayed in place of a retracted and subsequently removed Chapter.
\\n\\t
Chapters published due to inadvertent production mistakes shall be canceled and the cancellation notice will be published.
\\n
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2. STATEMENTS OF CONCERN
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A Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
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Authors refuse to approve a retraction proposed by the Academic Editor
\\n\\t
There is inconclusive evidence of scientific misconduct
\\n\\t
Authors and their respective institutions fail or refuse to provide adequate assistance in an investigation
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The publication of a Statement of Concern will adhere to the Retraction Notice guidelines outlined above
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An article PDF for which a Statement of Concern is published will remain available online without being edited or watermarked
\\n
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IntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
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3. CORRECTIONS
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A Correction will be issued by the Academic Editor when:
\\n\\n
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Only a small portion of a Chapter is flawed in a way that does not severely affect any findings.
\\n\\t
It is determined that the scientific community would be better served by a Correction rather than a Retraction.
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Corrections will be issued in one of two distinct forms -- ERRATUM or CORRIGENDUM, depending on the origin of a mistake.
\\n
\\n\\n
3.1. ERRATUM
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An Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
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A published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
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3.2. CORRIGENDUM
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A Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
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4. FINAL REMARKS
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IntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\\n\\n
In the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\\n\\n
The general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n
1. RETRACTIONS
\n\n
A Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\n
A formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\n
\n\t
Data fabrication
\n\t
Data recycling in a purportedly original research article
\n\t
Severe plagiarism - whether or not the plagiarism is to be deemed severe will be determined by the Academic Editor and verified by plagiarism checking software
\n\t
Double publication
\n\t
Copyright infringement - for example, if a Chapter uses copyrighted figures without permission.
\n\t
Unreliable findings
\n\t
Unethical research practices
\n\t
Any other practice or act considered potentially harmful to the scientific community.
\n
\n\n
Publishing of a Retraction Notice will adhere to the following guidelines:
\n\n\n\t
All relevant bibliographic information about a retracted Chapter will be given in the title.
\n\t
A Retraction Notice will be published as a regular book Chapter and will be given its own Chapter number.
\n\n\n
\n\t
Authors shall be required to approve a proposed retraction of their Chapter. If Authors maintain that their Chapter should not be retracted, the Academic Editor may issue a Statement of Concern (see 2. below).
\n
\n\n
1.2. REMOVALS AND CANCELLATIONS
\n\n
\n\t
Additionally, a Chapter retracted on grounds of copyright infringement (e.g. double publication) may be Removed by the publisher should the original copyright owner request such action. A Chapter retracted on grounds of its potential to harm the scientific community, for example, when a Chapter is defamatory in nature, may also be subject to removal.
\n\t
No formal Removal Notice will be published but a notice citing the reason for removal will be prominently displayed in place of a retracted and subsequently removed Chapter.
\n\t
Chapters published due to inadvertent production mistakes shall be canceled and the cancellation notice will be published.
\n
\n\n
2. STATEMENTS OF CONCERN
\n\n
A Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\n
\n\t
Authors refuse to approve a retraction proposed by the Academic Editor
\n\t
There is inconclusive evidence of scientific misconduct
\n\t
Authors and their respective institutions fail or refuse to provide adequate assistance in an investigation
\n\t
The publication of a Statement of Concern will adhere to the Retraction Notice guidelines outlined above
\n\t
An article PDF for which a Statement of Concern is published will remain available online without being edited or watermarked
\n
\n\n
IntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n
3. CORRECTIONS
\n\n
A Correction will be issued by the Academic Editor when:
\n\n
\n\t
Only a small portion of a Chapter is flawed in a way that does not severely affect any findings.
\n\t
It is determined that the scientific community would be better served by a Correction rather than a Retraction.
\n\t
Corrections will be issued in one of two distinct forms -- ERRATUM or CORRIGENDUM, depending on the origin of a mistake.
\n
\n\n
3.1. ERRATUM
\n\n
An Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\n
A published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n
3.2. CORRIGENDUM
\n\n
A Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n
4. FINAL REMARKS
\n\n
IntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\n
In the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\n
The general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
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He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. 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Radiotherapy and Nuclear Medicine Technology has always been my aspiration and my life. As years passed I accumulated a tremendous amount of skills and knowledge in Radiotherapy and Nuclear Medicine, Conventional Radiology, Radiation Protection, Bioinformatics Technology, PACS, Image processing, clinically and lecturing that will enable me to provide a valuable service to the community as a Researcher and Consultant in this field. My method of translating this into day to day in clinical practice is non-exhaustible and my habit of exchanging knowledge and expertise with others in those fields is the code and secret of success.",institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"313277",title:"Dr.",name:"Bartłomiej",middleName:null,surname:"Płaczek",slug:"bartlomiej-placzek",fullName:"Bartłomiej Płaczek",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/313277/images/system/313277.jpg",biography:"Bartłomiej Płaczek, MSc (2002), Ph.D. (2005), Habilitation (2016), is a professor at the University of Silesia, Institute of Computer Science, Poland, and an expert from the National Centre for Research and Development. His research interests include sensor networks, smart sensors, intelligent systems, and image processing with applications in healthcare and medicine. He is the author or co-author of more than seventy papers in peer-reviewed journals and conferences as well as the co-author of several books. He serves as a reviewer for many scientific journals, international conferences, and research foundations. Since 2010, Dr. Placzek has been a reviewer of grants and projects (including EU projects) in the field of information technologies.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"35000",title:"Prof.",name:"Ulrich H.P",middleName:"H.P.",surname:"Fischer",slug:"ulrich-h.p-fischer",fullName:"Ulrich H.P Fischer",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/35000/images/3052_n.jpg",biography:"Academic and Professional Background\nUlrich H. P. has Diploma and PhD degrees in Physics from the Free University Berlin, Germany. He has been working on research positions in the Heinrich-Hertz-Institute in Germany. Several international research projects has been performed with European partners from France, Netherlands, Norway and the UK. He is currently Professor of Communications Systems at the Harz University of Applied Sciences, Germany.\n\nPublications and Publishing\nHe has edited one book, a special interest book about ‘Optoelectronic Packaging’ (VDE, Berlin, Germany), and has published over 100 papers and is owner of several international patents for WDM over POF key elements.\n\nKey Research and Consulting Interests\nUlrich’s research activity has always been related to Spectroscopy and Optical Communications Technology. Specific current interests include the validation of complex instruments, and the application of VR technology to the development and testing of measurement systems. He has been reviewer for several publications of the Optical Society of America\\'s including Photonics Technology Letters and Applied Optics.\n\nPersonal Interests\nThese include motor cycling in a very relaxed manner and performing martial arts.",institutionString:null,institution:{name:"Charité",country:{name:"Germany"}}},{id:"341622",title:"Ph.D.",name:"Eduardo",middleName:null,surname:"Rojas Alvarez",slug:"eduardo-rojas-alvarez",fullName:"Eduardo Rojas Alvarez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/341622/images/15892_n.jpg",biography:null,institutionString:null,institution:{name:"University of Cuenca",country:{name:"Ecuador"}}},{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/215610/images/system/215610.jpeg",biography:"Muhammad Sarfraz is a professor in the Department of Information Science, Kuwait University. His research interests include computer graphics, computer vision, image processing, machine learning, pattern recognition, soft computing, data science, intelligent systems, information technology, and information systems. Prof. Sarfraz has been a keynote/invited speaker on various platforms around the globe. He has advised various students for their MSc and Ph.D. theses. He has published more than 400 publications as books, journal articles, and conference papers. He is a member of various professional societies and a chair and member of the International Advisory Committees and Organizing Committees of various international conferences. Prof. Sarfraz is also an editor-in-chief and editor of various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/267434/images/system/267434.jpg",biography:"Dr. Rohit Raja received Ph.D. in Computer Science and Engineering from Dr. CVRAMAN University in 2016. His main research interest includes Face recognition and Identification, Digital Image Processing, Signal Processing, and Networking. Presently he is working as Associate Professor in IT Department, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur (CG), India. He has authored several Journal and Conference Papers. He has good Academics & Research experience in various areas of CSE and IT. He has filed and successfully published 27 Patents. He has received many time invitations to be a Guest at IEEE Conferences. He has published 100 research papers in various International/National Journals (including IEEE, Springer, etc.) and Proceedings of the reputed International/ National Conferences (including Springer and IEEE). He has been nominated to the board of editors/reviewers of many peer-reviewed and refereed Journals (including IEEE, Springer).",institutionString:"Guru Ghasidas Vishwavidyalaya",institution:{name:"Guru Ghasidas Vishwavidyalaya",country:{name:"India"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:null},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:null,institution:{name:"Beijing University of Technology",country:{name:"China"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Igor Victorovich Lakhno was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPh.D. – 1999, Kharkiv National Medical Univesity.\nDSC – 2019, PL Shupik National Academy of Postgraduate Education \nProfessor – 2021, Department of Obstetrics and Gynecology of VN Karazin Kharkiv National University\nHead of Department – 2021, Department of Perinatology, Obstetrics and gynecology of Kharkiv Medical Academy of Postgraduate Education\nIgor Lakhno has been graduated from international training courses on reproductive medicine and family planning held at Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor in the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics, and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s been a professor in the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics, and gynecology department. He’s affiliated with Kharkiv Medical Academy of Postgraduate Education as a Head of Department from November 2021. Igor Lakhno has participated in several international projects on fetal non-invasive electrocardiography (with Dr. J. A. Behar (Technion), Prof. D. Hoyer (Jena University), and José Alejandro Díaz Méndez (National Institute of Astrophysics, Optics, and Electronics, Mexico). He’s an author of about 200 printed works and there are 31 of them in Scopus or Web of Science databases. Igor Lakhno is a member of the Editorial Board of Reproductive Health of Woman, Emergency Medicine, and Technology Transfer Innovative Solutions in Medicine (Estonia). He is a medical Editor of “Z turbotoyu pro zhinku”. Igor Lakhno is a reviewer of the Journal of Obstetrics and Gynaecology (Taylor and Francis), British Journal of Obstetrics and Gynecology (Wiley), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for a DSc degree “Pre-eclampsia: prediction, prevention, and treatment”. Three years ago Igor Lakhno has participated in a training course on innovative technologies in medical education at Lublin Medical University (Poland). Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: are obstetrics, women’s health, fetal medicine, and cardiovascular medicine. \nIgor Lakhno is a consultant at Kharkiv municipal perinatal center. He’s graduated from training courses on endoscopy in gynecology. He has 28 years of practical experience in the field.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. RELACION DE PONENCIAS DE LA SOCIEDAD ESPAÑOLA DE OFTALMOLOGIA. 10/2014.",institutionString:null,institution:null},{id:"265335",title:"Mr.",name:"Stefan",middleName:"Radnev",surname:"Stefanov",slug:"stefan-stefanov",fullName:"Stefan Stefanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/265335/images/7562_n.jpg",biography:null,institutionString:null,institution:null},{id:"243698",title:"Dr.",name:"Xiaogang",middleName:null,surname:"Wang",slug:"xiaogang-wang",fullName:"Xiaogang Wang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243698/images/system/243698.png",biography:"Dr. Xiaogang Wang, a faculty member of Shanxi Eye Hospital specializing in the treatment of cataract and retinal disease and a tutor for postgraduate students of Shanxi Medical University, worked in the COOL Lab as an international visiting scholar under the supervision of Dr. David Huang and Yali Jia from October 2012 through November 2013. Dr. Wang earned an MD from Shanxi Medical University and a Ph.D. from Shanghai Jiao Tong University. Dr. Wang was awarded two research project grants focused on multimodal optical coherence tomography imaging and deep learning in cataract and retinal disease, from the National Natural Science Foundation of China. He has published around 30 peer-reviewed journal papers and four book chapters and co-edited one book.",institutionString:null,institution:null},{id:"7227",title:"Dr.",name:"Hiroaki",middleName:null,surname:"Matsui",slug:"hiroaki-matsui",fullName:"Hiroaki Matsui",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Tokyo",country:{name:"Japan"}}},{id:"318905",title:"Prof.",name:"Elvis",middleName:"Kwason",surname:"Tiburu",slug:"elvis-tiburu",fullName:"Elvis Tiburu",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Ghana",country:{name:"Ghana"}}},{id:"336193",title:"Dr.",name:"Abdullah",middleName:null,surname:"Alamoudi",slug:"abdullah-alamoudi",fullName:"Abdullah Alamoudi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"318657",title:"MSc.",name:"Isabell",middleName:null,surname:"Steuding",slug:"isabell-steuding",fullName:"Isabell Steuding",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Harz University of Applied Sciences",country:{name:"Germany"}}},{id:"318656",title:"BSc.",name:"Peter",middleName:null,surname:"Kußmann",slug:"peter-kussmann",fullName:"Peter Kußmann",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Harz University of Applied Sciences",country:{name:"Germany"}}},{id:"338222",title:"Mrs.",name:"María José",middleName:null,surname:"Lucía Mudas",slug:"maria-jose-lucia-mudas",fullName:"María José Lucía Mudas",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Carlos III University of Madrid",country:{name:"Spain"}}}]}},subseries:{item:{id:"4",type:"subseries",title:"Fungal Infectious Diseases",keywords:"Emerging Fungal Pathogens, Invasive Infections, Epidemiology, Cell Membrane, Fungal Virulence, Diagnosis, Treatment",scope:"Fungi are ubiquitous and there are almost no non-pathogenic fungi. Fungal infectious illness prevalence and prognosis are determined by the exposure between fungi and host, host immunological state, fungal virulence, and early and accurate diagnosis and treatment. \r\nPatients with both congenital and acquired immunodeficiency are more likely to be infected with opportunistic mycosis. Fungal infectious disease outbreaks are common during the post- disaster rebuilding era, which is characterised by high population density, migration, and poor health and medical conditions.\r\nSystemic or local fungal infection is mainly associated with the fungi directly inhaled or inoculated in the environment during the disaster. The most common fungal infection pathways are human to human (anthropophilic), animal to human (zoophilic), and environment to human (soilophile). Diseases are common as a result of widespread exposure to pathogenic fungus dispersed into the environment. \r\nFungi that are both common and emerging are intertwined. In Southeast Asia, for example, Talaromyces marneffei is an important pathogenic thermally dimorphic fungus that causes systemic mycosis. Widespread fungal infections with complicated and variable clinical manifestations, such as Candida auris infection resistant to several antifungal medicines, Covid-19 associated with Trichoderma, and terbinafine resistant dermatophytosis in India, are among the most serious disorders. \r\nInappropriate local or systemic use of glucocorticoids, as well as their immunosuppressive effects, may lead to changes in fungal infection spectrum and clinical characteristics. Hematogenous candidiasis is a worrisome issue that affects people all over the world, particularly ICU patients. CARD9 deficiency and fungal infection have been major issues in recent years. Invasive aspergillosis is associated with a significant death rate. 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International Union of Microbiological Societies (IUMS) Fellow, and International Emerging Infectious Diseases (IEID) Fellow, Centers for Diseases Control and Prevention (CDC), Atlanta, USA. Diploma of Dermatological Scientist, Japanese Society for Investigative Dermatology. Ph.D. of Juntendo University, Japan. Bachelor’s and Master’s degree, Medicine, West China University of Medical Sciences. Chair of Sichuan Medical Association Dermatology Committee. General Secretary of The 19th Annual Meeting of Chinese Society of Dermatology and the Asia Pacific Society for Medical Mycology (2013). In charge of the Annual Medical Mycology Course over 20-years authorized by National Continue Medical Education Committee of China. Member of the board of directors of the Asia-Pacific Society for Medical Mycology (APSMM). Associate editor of Mycopathologia. Vice-chief of the editorial board of Chinses Journal of Mycology, China. 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