The solubility of isoflavones in pure SCCO2 without ethanol at 313K, 25MPa
1. Extraction of soybean isoflavone
1.1. Soybean isoflavones and attractive potential of supercritical carbon dioxide (SCCO2)
Isoflavones produced from bioresources are gaining attention as attractive components in food supplements. Isoflavones are heterocyclic phenols with a structure very similar to that of estrogens. Isoflavone displays like estrogens and has anti estrogen activity; it influences sex hormone metabolism and related biological activity [1,2] and prevents osteoporosis [3,4], arteriosclerosis , dementia , and cancer [6,7].
Soybeans contain 12 different isoflavones classified into two components, glycosides and aglycons. Glycoside isoflavone has a glucose chain in its molecular structure; aglycon isoflavone does not have a glucose structure.
Ninety-three percent of isoflavones are produced and stored as glycoside. Therefore, in practical separation processes, glycoside isoflavones were the major fraction and were recognized as the main target group rather than aglycons. This article focuses on daidzin, genistin and glycitin as typical glycosides. Their aglycons (i.e., daidzein, genistein and glycitein) were examined for comparison. The aglycons have no glycoside chain; their chemical structure is depicted in Fig. 1.
Methods of extracting isoflavones from soybean have been previously examined by using organic solvent , pressurized liquid , ultrasound [10,11], and supercritical carbon dioxide [12-16]. Supercritical carbon dioxide has been the favorite extraction medium for many food functional components, i.e. caffeine [17-20], capsaicin [21,22], carotenoids [23-26], polyphenol [27-30], aspirin , and coenzyme Q10 .
In general, the solubility of polar components in the SCCO2-only system was very low because carbon dioxide has non-polar characteristics. The solubility of polar components has been well enhanced by adding polar components to the SCCO2 system. The added component was referred to as an entrainer. Ethanol was effectively employed as an entrainer for extraction and applied to caffeine [17,19], capsaicin , catechin , epicatechin , aspirin, and coenzyme Q10 . Rostagno et al. (2002) successfully extracted large amounts of isoflavones from soybean flour by using methanol aqueous solution as an entrainer . Zuo et al. (2008) also extracted isoflavones from soybean meal by using methanol .
To design practical separation processes using SCCO2, it is necessary to establish a reliable database of the entrainer’s enhancement effects. This would facilitate both the choice of a suitable entrainer for an objective component and the quantitative evaluation of separation yield of a target component in actual processes.
In this chapter, we demonstrate the solubility of isoflavones in SCCO2 with ethanol added. The solubility in an SCCO2-only system was also measured for comparison. The effect of the entrainer on solubility is discussed with the hydrophobicity of guest components evaluated from their molecular structure. The thermodynamic relationship between the solubility and the parameter indicated a non-ideal state in SCCO2 .
1.2. Solubility of isoflavones and effect of entrainer
A circulation flow of SCCO2 was employed for the experimental extraction system (JASCO Co., Ltd., Tokyo) as presented in Fig. 2. The 1.0mL stainless-steel extraction vessel was installed in an extraction line with a total volume of 19.8mL. The extraction temperature was set at 313K. The pressure range was from 15 to 25MPa. The CO2 volumetric flow rate in the extraction line was adjusted to a constant 5mL/min at 15MPa and 25MPa.
1.2.2. Solubility of isoflavones
Table 1 summarizes the solubility of isoflavones in the SCCO2-single system. In general, the isoflavones were hardly extracted by the SCCO2-single system. In particular, the solubility of glycoside isoflavones was very low it could not be detected by HPLC.
|Aglycon||Daidzein||5.14 x 10-10|
|Genistein||6.38 x 10-10|
1.2.3. Effect of entrainer (ethanol) on solubility of isoflavones
Figure 3 presents the solubility of daidzin (as glycoside) and daidzein (as aglycon) in the SCCO2 and ethanol binary system. Solubility S was increased remarkably by increasing the molar fraction of ethanol, M. This trend was also obtained at 25MPa. The solubility of genistin (as glycoside) and genistein (as aglycon) presented in Fig. 4 also exhibited the same trend. This remarkable influence of the molar fraction of ethanol also seemed to be similar between glycitin (as glycoside) and glycitein (as aglycon) (Fig. 5). The results indicated that the solubility of hydrophilic glycoside isoflavones (daidzin, genistin, and glycitin) depended more strongly on the molar fraction of ethanol.
As seen in Fig. 3, the solubility of daidzin at 25MPa was far greater than that at 15MPa. Ethanol depended more heavily on the molar fraction at 25MPa than at 15MPa. In contrast to genistin and genistein, the solubility was almost the same in spite of the increased pressure (Fig. 4). The dependency on the molar fraction of ethanol was similar for 25MPa and 15MPa.
The solubility ratio was defined as the solubility of 25 MPa divided by that of 15 MPa. The molar fraction of ethanol was set at 0.10, as evaluated from Figs. 3 and 4, and summarized in Table 2. In the case of daidzin, the solubility ratio was calculated as 6.3 fold. It was especially high among the tested isoflavones, i.e. 1.8 (Genistin), 1.3 (Daidzein), and 1.8 (Genistein). The solubility of daidzin was strongly affected by extraction pressure in four tested isoflavones.
The solubility of daidzin (glycoside) exceeded that of daidzein (aglycon). This trend appeared especially strong in daidzin, in contrast to that of other isoflavones. For other isoflavones, glycosides (genistin and glycitin) were less soluble than the corresponding aglycons (genistein and glycitein) due to their hydrophilic nature and the glycoside chain in their molecular structure. The detailed reasons for the special behavior of daidzin and daidzein are not clear at present.
The enhanced solubility after adding ethanol was preliminarily evaluated by the logarithmic dependency of α on the molar fraction of ethanol M. The solubility S was proportional to the α th. power of M as indicated in empirical equation Eq. (1).
The power term α is summarized in Table 3. The power term α of glycoside isoflavones (daidzin, genistin, glycitin) at both 15MPa and 25MPa often exceeded 3.0. As presented in Table 4, glycoside isoflavones are commonly more hydrophilic than their corresponding aglycons. Additive ethanol concentration in SCCO2 strongly affected the solubility of hydrophilic isoflavones. The power term α of daidzein (aglycon) exceptionally exceeded 3.0 in spite of its hydrophobic nature. The detailed mechanism of solubilization must be investigated further. It may be related to a slight difference of molecular structure.
|Glycoside||Daidzin||3.42 R2=0.708||3.69 R2=0.907|
|Genistin||3.08 R2=0.996||3.41 R2=0.932|
|Glycitin||No data||3.27 R2=0.852|
|Aglycon||Daidzein||3.17 R2=0.967||3.02 R2=0.975|
|Genistein||0.728 R2=0.985||1.72 R2=0.999|
|Glycitein||No data||1.85 R2=0.988|
|R2 : Correlation coefficient|
The dependency on the molar fraction of ethanol increased at higher pressures. The solubilities of genistin and genistein are almost the same in spite of the pressure change. The dependency on molar fraction of ethanol was also similar, suggesting that the solubility depended heavily on the amount of ethanol added. Power term α became large under SCCO2 at higher pressures, except for daidzein.
Solubilities of six different isoflavones were measured in an SCCO2 system with ethanol added. Ethanol effectively increased the solubility of isoflavones. It served as an attractive entrainer with SCCO2. The power term in the molar fraction of ethanol exceeded 3.0. The enhancement was remarkable in more hydrophilic isoflavones (daidzin, genistin, and glycitin). We experimentally determined the hydrophobicity (Log P)  of isoflavones from the equilibrium constant between 1-octanol and water. The hydrophobicity of daidzin was lowest among the tested isoflavones, and the enhancement due to adding ethanol was the highest.
Soybean and other natural bioresources are abundant sources of various glycoside isoflavones. Isoflavones will be successfully extracted from these sources for practical application by SCCO2 with ethanol added.
2. Enzymatic modification of soybean lipid by lipase and immobilized lipase
Soybean is beneficial in food applications and is attractive as a bioresource for functional components. Soybean contains many proteins and much oil. Furthermore, many functional components, isoflavone , lecithin , saponin [35,37], and oligosaccharide, [38,39] are desirable for promoting human health.
Soybean oil generally contains 52% linoleic acid, 22% oleic acid, 10% palmitic acid, and 8% linolenic acid. Soybean oil can be readily hydrolyzed by lipase like other vegetable oils. The produced fatty acids have several applications such as in manufacturing soaps, surfactants, and detergents, and in food.
Lipases have received attention for lipid modification [40-42]. They are used in fields such as food engineering, detergents, beverages, cosmetics, biomedical uses, and the chemical industry. They catalyze hydrolysis, alcoholysis, acidolysis, amidolysis, and esterification in the food and pharmaceutical industries [43-48]. Lipid modifications (hydrolysis, esterification, etc.) often lead to better quality products due to high specificity and selectivity of the lipase. Immobilized lipases have been applied in various hydrophobic reactions [42,49-51]. Reactivity of immobilized lipase was affected by physicochemical factors in reaction media [52,53]. A hydrophobic material is especially favorable for quick initiation of hydrophobic enzymatic reaction due to the easy diffusion of the substrate in the inner pores of the carrier. Previously, hydrophilic gels and solid porous carriers were often employed even for hydrophobic substrate reactions. Detailed technical data focused on carriers to quickly initiate hydrophobic enzymatic reactions, and high yield repeated-use immobilized enzymes are necessary in industrial design of hydrophobic enzyme reactions [54-56].
2.2. Process chemistry of soybean oil modification
Vegetable oils (olive oil [40,42]) can be hydrolyzed to produce monoglyceride, diglyceride, free fatty acids, and glycerol. Free fatty acids are value-added products because of their wide applications in surfactants, soap manufacturing, the food industry, and biomedical uses. The conventional and industrial method of oil hydrolysis has been carried out using a chemical catalyst at high temperatures and pressure. However, successful enzymatic hydrolysis reactions are possible without high temperatures and pressure.
Dalla, R. C. et al. investigated the continuous production of fatty acid ethyl esters from soybean oil in compressed fluids, namely carbon dioxide, propane, and n-butane, using immobilized Novozym 435 as a catalyst . Their work evaluated the effects of some process variables on the production of fatty acid ethyl esters from soybean oil in compressed propane using Novozym 435 as a catalyst in a packed-bed reactor. In contrast to using carbon dioxide and n-butane, their results indicated that lipase-catalyzed alcoholysis was achieved in a continuous tubular reactor in compressed propane with high reaction yields at mild temperatures (70°C) and pressures (60 bar) and with short reaction times. The results demonstrated that lipase-catalyzed alcoholysis in a packed-bed reactor using compressed propane as solvent was promising as a potential alternative to conventional processes. It may be possible to manipulate process variables as well as reactor configurations to achieve acceptable yields.
Guan, F. et al. investigated the transesterification of a combination of two lipases . A combination of two lipases was employed to catalyze methanolysis of soybean oil in an aqueous medium during production process. The aqueous medium was a mixture of 7 g soybean oil, methanol in various molar ratios (3:1, 4:1, 5:1, 6:1, and 9:1; methanol : oil) and 2mL (550U per mL)
In hydrolysis, water is used to break the bonds of certain substances. In biotechnology and living organisms, these substances are often polymers. In hydrolysis involving an ester link between two amino acids in a protein, the products include the hydroxyl (OH) group, which becomes carboxylic acid with the addition of the remaining proton.
Hydrolysis reactions in living organisms are performed with the help of catalysis by a class of enzymes known as hydrolases. The biochemical reactions that break down polymers such as proteins (peptide bonds between amino acids), nucleotides, complex sugars and starch, and fats are catalyzed by hydrolases. Within this class, lipases, amylases, and proteinases hydrolyze fats, sugars and proteins, respectively (Fig. 6).
The hydrolysis of vegetable oils is also industrially important. The complete hydrolysis of triglycerides will produce fatty acids and glycerol. These fatty acids find several applications such as in manufacturing soaps, surfactants, and detergents, and in the food industry. Since there are many kinds of natural substrates, the high specificity and selectivity of the enzymes used in the hydrolysis reaction will lead to products of better quality. Lipase has been used in the hydrolysis of different oils and fats to produce free fatty acids.
Ting, W-J. et al. investigated soybean hydrolysis by immobilized lipase in chitosan beads . Their work is the culmination of their research efforts to develop an enzymatic/acid-catalyzed hybrid process for production with a view to utilizing edible and off-quality soybean oils as feedstock. They achieved a higher degree of hydrolysis. The reaction was carried out at 40°C for 12 h using binary immobilized
Esterification is the chemical process of making esters, which are compounds of the chemical structure R-COOR', where R and R' are either alkyl or aryl groups (Fig. 7). The esterification process has a broad spectrum of uses from preparing highly specialized esters in chemical laboratories to producing millions of tons of commercial ester products. These commercial compounds are manufactured by either a batch or a continuous synthetic process. The batch procedure involves a single pot reactor that is filled with the acid and alcohol reactants.
Sugar fatty acid esters are widely used as non-ionic surfactants in cosmetic and food applications. Current chemical production is based on high-temperature esterification of sugars and fatty acids, using an alkaline catalyst leading to a mixture of products. Alternatively, sugar fatty acid esters can be obtained by fermentation as so-called biosurfactants. The direct esterification of sugar and fatty acid using isolated enzymes (mainly lipases) is hampered by the low solubility of sugars in most organic solvents. Good conversions can be achieved in pyridine, but this solvent is incompatible with food applications. Other solutions are based on the use of alkylglycosides or protected sugars like isopropylidene or phenylboronic acid derivatives, which require additional synthesis steps.
Nagayama, K. et al. investigated lecithin microemulsion-based organogels as immobilization carriers for the esterification of lauric acid with butyl alcohol catalyzed by
2.3. Immobilized enzymatic reaction of soybean lipid modification
Immobilization of lipase has been investigated to improve the stability and reusability of lipase in oil hydrolysis. For practical applications, a systematic strategy is necessary to select suitable support and organic solvents. Authors investigated a key factor of suitable support to improve enzyme activity and stability of immobilized lipase .
Immobilized enzymes have been examined for various industrial applications. In general, enzyme immobilization effectively enables separating the enzyme from products, thus facilitating their recovery and repeated use [40,42,62]. This is promising for industrial enzymatic production of various biomaterials. The main aspects of the currently investigated immobilized enzyme are as follows. First, the molecular structure of the enzyme is directly influenced by immobilization . Second, enzyme reactivity is affected by the physicochemical characteristics of the enzyme carrier and the reaction media [40,51]. To quickly initiate hydrophobic enzymatic reactions, a water-in-oil (W/O) microemulsion system is desirable for achieving higher concentrations of hydrophobic substrate in the reaction media. Third, the diffusion of the substrate and the reaction products determines the rate-limiting condition in the reactivity of the immobilized enzyme . Finally, repeated use of the immobilized enzyme in a practical process is a key factor in reducing costs in industrial applications.
Solid porous carriers are expected to resist compaction and deformation of carrier particles during practical use in bioreactors. Hydrophobic solid porous materials are preferred as immobilized enzymes for hydrophobic reactions. Table 5 summarizes previous hydrophobic substrate reactions using immobilized lipase. Hydrophobic materials, primarily a polypropylene porous commercial carrier called Accurel, have been employed for lipid hydrolysis and esterification. Lipase is adsorbed with strong multipoint interactions in Accurel . Particle size plays a dominant role in determining the rate-limiting condition of the substrate [46,49,55,74]. The particle size as well as handling of particles was very important for both the practical design of the bioreactor and for determining reaction-rate-limiting conditions. In the Accurel EP-100 system, the effect of particle size on reaction rate was examined for a size range of 0.2 to 2.5 mm [49,55,62,74]. A higher reaction rate was obtained for a smaller immobilized carrier. Sabbani et al. reported that the reaction rate was increased six-fold by decreasing the particle size from 0.2 to 1.5 mm. Montero et al. pointed out that cross-linking of lipase (
2.3.1. W/O microemulsion
W/O microemulsions are spontaneous aggregates composed of amphiphilic molecules in non-polar media. The properties of reverse micelles have been extensively investigated in the field of reverse micellar techniques. Reverse micelles enable hydrophilic proteins to be solubilized in organic solvent and are anticipated to be used as separation and enzymatic reaction media with hydrophobic substrates. When enzymes are micro-encapsulated, they are situated inside the water pool of the W/O microemulsion; whether or not they interact with the micellar interface depends on the enzyme species (Fig. 8). For example, an enzyme reaction involving lipase was observed on the interfacial layer between the hydrophobic phase containing substrates, and the hydrophilic phase containing dissolved lipase.
Uehara et al. defined the reaction condition producing high reactivity over a limited range of both hydrophilicity and interfacial fluidity of the microemulsion droplet . Their reaction condition was identified as the most favorable condition for sugar–ester alcohol W/O microemulsion media to perform lipid hydrolysis. The critical micelle concentration depended on the concentration of 1-butanol and was found to be inversely proportional to the second power of the 1-butanol concentration. The initial reaction rate of the hydrolysis of triolein in W/O microemulsion depended on the solubilized water content, reaching a maximum in the limited range of 2 < Wsoln< 4. The maximum initial reaction rate increased about 2-fold following the addition of 1-butanol. The most favorable concentration of 1-butanol for hydrolysis by
Naoe et al. investigated the esterification of oleic acid with octyl alcohol catalyzed by
2.3.2. Gel beads carrier
The major problem that must be solved to employ a microemulsion system in industrial processes is the recovery of the products and the repeated use of enzyme. Usual techniques such as extraction and distillation lead to poor separation because of the problems of emulsion-forming and foaming caused by the presence of surfactants. One approach to simplifying the recovery of the product and the enzyme for reuse from microemulsion based-media has been to employ gelled microemulsion systems. Interestingly, many W/O microemulsions can be gelled by adding gelatin, yielding a matrix suitable for enzyme immobilization. Cooling at room temperature causes a transparent gel with reproducible physical properties to form. These enzyme-containing, gelatin-based gels are rigid and stable in various non-polar organic solvents and may therefore be used for biotransformations in organic media. Under most conditions, the gel matrix fully retains the surfactant, gelatin, water, and enzyme components, allowing the diffusion of non-polar substrates or products between a contacting non-polar phase and the gel pellets.
Natural gelling agents such as gelatin, agar and κ-carrageenan have been tested for the formation of lecithin microemulsion-based gels as well as hydrogels presented by Stamatis, H and Xenakis, A . Lipase-containing microemulsions-based organogels formulated with various biopolymers have considerable potential for their application in biotransformations. Lipase immobilized in gelatin and agar organogels exhibited good stability in catalyzing esterification reactions under mild conditions with high conversion yields. High yields (80%) were obtained with agar and κ-carrageenan organogels in isooctane. The remaining lipase activity in repeated syntheses was found to depend on the nature of the biopolymer used for forming the organogels. Gelatin and agar microemulsion-based gels had the highest operational stability. Moreover, aqueous gelatin and agar gels containing only lipase, water, and biopolymer retain their integrity in organic solvents and can also be used for the synthesis of esters.
Chitosan, poly [β-(1-4)-linked-2-amino-2-deoxy-D-glucose], is non-toxic, hydrophilic, biocompatible, biodegradable, and anti-bacterial and can be used as a material for immobilized carriers since it has a variety of functional groups that can be tailored to specific applications. Xie, W. and Wang, J. investigated the effects of various transesterification parameters on the enzymatic conversion of soybean oil . In their work, magnetic chitosan microspheres were prepared by the chemical co-precipitation approach using glutaraldehyde as the cross-linking reagent for lipase immobilization. Using the immobilized lipase, the conversion of soybean oil to fatty acid methyl esters reached 87% under the optimized conditions of a methanol/oil ratio of 4:1 with the three-step addition of methanol, reaction temperature 35°C, and reaction time 30h. Moreover, the immobilized lipase could be used for four times without significant decrease of activity.
2.3.3. Polypropylene carrier
The immobilized lipase (
The amount of immobilized lipase per unit mass of particle was increased by 19% in smaller particles (500 to 840 μm). The immobilized yield lipase based on the adsorbed amount was high (over 98%) in every class of particle size (Fig. 10). Cross-linking of lipase by glutaraldehyde (GA) holds much promise for immobilization.
The reactivity of immobilized lipase as evaluated from the oleic acid production rate strongly depended on the Accurel particle size. In particular, the 500 to 840 μm (mean diameter 670μm) particles performed significantly outstanding reactivity compared with that of 840 to 1180 μm (mean diameter 1010μm) particles and original Accurel (Fig. 11). The experimental effectiveness factor was obtained and compared with the theoretical effectiveness factor. The difference was speculated to be due to assumptions of the geometrical factor of particles and the partition equilibrium of the substrate between the carrier particle and bulk phase. Quick initiation was observed in the repeated use of immobilized lipase on the 500 to 840 μm particles. The production yield was well-preserved.
2.3.4. Nanofiber membrane
Li, S-F. and Wu, W-T. investigated immobilized lipase activity using a nanofiber membrane . The activity retention of the immobilized lipase was 87.5% of the free enzyme. Under these optimal reaction conditions, the hydrolysis conversion of soybean oil was 72% after 10min and 85% after 1.5h. In reusability, the immobilized lipase retained 65% of its initial conversion after 20 additional batch reactions. Protein loading reached 21.2mg/g material of the membrane due to the large specific surface area provided by the nanofibers. This effective enzyme immobilization method has good potential for industrial applications.
Soybean has been expected to be used both as a food and as a bioresource for attractive functional components. Soybean contains many proteins and much oil. Soybean oil can be hydrolyzed readily by lipase like other vegetable oils. The produced fatty acids find several applications such as in manufacturing soaps, surfactants, and detergents, and in food.
Immobilization of lipase has been investigated to improve its stability and reusability in oil hydrolysis. For practical applications, a systematic strategy is necessary to select suitable support and organic solvent. Since the novel developed method is promising, it could be used industrially for producing chemicals requiring immobilized lipases.
Log P: hydrophobicity index by Laane et al. . P was defined by partition equilibrium (-)
M: molar fraction of ethanol in SCCO2, referred from  ([mol-Ethanol]/[mol-(SCCO2+Ethanol)])
S: molar fraction of extracted sample in the SCCO2 and ethanol binary system, referred from  ] ([mol-extracted sample]/[mol-(SCCO2+Ethanol)])
Wsoln: molar ratio of solubilized water to amphiphile, referred from  ([mol-H2Osoln]/[mol-amphiphile])
α: the power term on the molar fraction of ethanol M, presented by Eq. (1), referred from . It is summarized in Table 3 (-)
Izumi T. Obata A. Arii M. Yamaguchi H. Matsuyama A. 2007 Oral Intake of Soy Isoflavone Improves the Aged skin of Adult Women. 53 57 62
Lee Y. B. Lee H. J. Won M. H. Hwang I. K. Kang T. C. Lee J. Y. Nam S. Y. Kim K. S. Kim E. Cheon S. H. Sohn H. S. 2004 Soy isoflavones Improve Spatial Delayed Matching-to-Place Peformance and Reduce Cholinergic Neuron Loss in Elderly Male Rats. 134 1827 1831
Lee Y. B. Lee H. J. Kim K. S. Lee J. Y. Nam S. Y. Cheon S. H. Sohn H. S. 2004 Evaluation of the preventive Effect of Isoflavone Extract on Bone Loss in Ovariectomized Rats. 68 5 1040 1045
Suh K. S. Koh G. Park C. Y. Woo J. T. Kim S. W. Kim J. W. Park I. K. Kim Y. S. 2003 Soybean isoflavones inhibit tumor facter-α-induced apoptosis and production of interleukin-6 and prostaglandin E2 in osteoblastic cells. 63 209 215
Mahesha H. G. Singh S. A. Rao A. G. A. 2007 Inhibition of lipoxygenase by soy isoflavones: Evidence of isoflavones as redox inhibitors 461 176 185
Chan H. Y. Chan H. Y. Leung L. K. 2003 A potential mechanism of soya isoflavoes against 7,12-dimetylbenz[a]anthracene tumour inhibition. 90 457 465
Clubbs E. A. Bomser J. A. 2007 Glycitein activates extracellular signal-regulated kinase via vascular endothelial growth factor receptor signaling in nontumorigenic (RWPE-1) prostate epithelial cells 18 525 532
Murphy P. A. Barua K. Hauck C. C. 2002 Solvent extraction selection in the determination of isoflavones in soy foods. 777 129 138
Luthria D. L. Biswas R. Natarajan S. 2007 Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean 105 325 333
Rostagno M. A. Palma M. Barroso C. G. 2003 Ultrasound-assisted extraction of soy isoflavones. 1012 119 128
Rostagno M. A. Palma M. Barroso C. G. 2007 Ultrasound-assisted extraction of isoflavones from beverages blended with fruit juices. 597 265 272
Araújo J. M. A. Silva M. V. Chaves J. B. P. 2007 Supercritical fluid extraction of daidzein and genistein isoflavones from soybean hypocotyls after hydrolysis with endogenous β-glucosidases. 105 266 272
Kao T. H. Chien J. T. Chen B. H. 2008 Extraction yield of isoflavones from soybeans cake as affected by solvent and supercritical carbon dioxide. 107 1728 1736
Rostagno M. A. Araújo J. M. A. Sandi D. 2002 Supercritical fluid extraction of isoflavones from soybean flour 78 111 117
Yu J. Liu Y. F. Qiu A. Y. Wang X. G. 2007 Preparation of isoflavones enriched soy protein isolate from defatted soy hypocotyls by supercritical CO2 40 800 806
Zuo Y. B. Zeng A. W. Yuan X. G. Yu K. T. 2008 Extraction of soybean isoflavones from soybean meal with aqueous methanol modified supercritical carbon dioxide 89 384 389
Iwai Y. Nagano H. Lee G. S. Uno M. Arai Y. 2006 Measurement of entrainer effect of water and ethanol on solubility in supercritical carbon dioxide by FT-IR spectroscopy. 38 312 318
Johannsen M. Brunner G. 1994 Solubility of the xanthenes caffeine, theophylline and theobromine in supercritical carbon dioxide. 95 215 226
Kopcak U. Mohamed R. S. 2005 Caffeine solubilities in supercritical carbon dioxide/co-solvent mixtures. 34 209 214
Li S. Varadarajan G. S. Hartland S. 1991 Solubility of theobromine and caffeine in supercritical carbon dioxide: correlation with density-based models. 68 263 280
Duarte C. M. M. Crew M. Casimiro T. Aguiar-Ricardo A. Ponte M. N. 2002 Phase equilibrium for capsaicin + water + ethanol + supercritical carbon dioxide. 22 87 92
de la Fuente J. C. Valderrama J. O. Bottini S. B. del Valle J. M. 2005 Measurement and modeling of solubilities of capsaicin in high-pressure CO2 34 195 201
Cygnarowicz M. L. Maxwell R. J. Selder W. D. 1990 Equilibrium solubility of β-carotene in supercritical carbon dioxide 59 57 71
de la Fuente J. C. Oyarzún B. Quezada N. del Valle J. M. 2006 Solubility of carotenoid pigment (lycopene and astaxanthin) in supercritical carbon dioxide. 247 90 95
Škerget M. Knez Ž. Habulin M. 1995 Solubility of β-carotene and oleic acid in dense CO2 and data correlation by a density based model.Fluid Phase Equilibria. 109 131 138
Subra P. Castellani S. Ksibi H. Garrabos Y. 1997 Contribution to the determination of the solubility of β-carotene in supercritical carbon dioxide and nitrous oxide: experimental data and modeling. 131 269 286
Berna A. Cháfer A. Montón J. B. Subirats S. 2001 High-pressure solubility data of system ethanol (1) + catechin (2) + CO2 (3). 20 157 162
Cháfer A. Berna A. Montón J. B. Nuñoz R. 2002 High-pressure solubility data of system ethanol (1) + epicatechin (2) + CO2 (3). 24 103 109
Nunes A. V. M. Matias A. A. da Ponte M. N. Duarte C. M. 2007 Quaternary Phase Equilibria for scCO2 + Biophenolic Compound + Water + Ethanol. 52 244 247
Wang L. H. Cheng Y. Y. 2005 Solubility of Puerarin in Ethanol + Supercritical Carbon Dioxide 50 1747 1749
Huang Z. Chiew Y. C. Lu W. D. Kawi S. 2005 Solubility of aspirin in supercritical carbon dioxide/alcohol mixture. 237 9 15
Matias A. A. Nunes A. V. M. Casimiro T. Duarte C. M. M. 2004 Solubility of coenzyme Q10 in supercritical carbon dioxide 28 201 206
Nakada M. Imai M. Suzuki I. 2009 Impact of ethanol addition on the solubility of various soybean isoflavones in supercritical carbon dioxide and the effect of glycoside chain in isoflavones 95 564 571
Laane C. Boeren S. Vos K. Veeger C. 1987 Rules for optimization of biocatalysis in organic solvents. 30 81 87
Paucar-Menacho L. M. Amaya-Farfan J. Berhow M. A. Mandarino J. M. G. Mejia E. G. Chang Y. K. 2010 A high-protein soybean cultivar contains lower isoflavones and saponins but higher minerals and bioactive peptides than a low-protein cultivar 120 15 21
Comas D. I. Wagner J. R. Tomas M. C. 2006 Creaming stability of oil in water (O/W) emulsions: Influence of pH on soybean protein-lecithin interaction 20 990 996
Berhow M. A. Wagner E. D. Vaughn S. F. Plewa M. J. 2000 Characterization and antimutagenic activity of soybean saponins. 448 11 22
Viana P. A. Rezende S. T. Falkoski D. L. Leite T. A. Jose I. C. Moreira M. A. Guimaraes V. M. 2007 Hydrolysis of oligosaccharides in soybean products by Debaryomyces hansenii UFV-1 α-galactosidases. 103 331 337
Wang Q. Ying T. Jahangir M. M. Jiang T. 2012 Study on removal of coloured impurity in soybean oligosaccharides extracted from sweet slurry by adsorption resins
Cao L. Bornscheuer U. T. Schmid R. D. 1999 Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme 6 279 285
Kiatsimkul-P P. Sutterlin W. R. Suppes G. J. 2006 Selective hydrolysis of epoxidized soybean oil by commercially available lipases: Effects of epoxy group on the enzymatic hydrolysis 41 55 60
Virto M. D. Agud I. Montero S. Blanco A. Solozabal R. Lascaray J. M. Llama M. J. Serra J. L. Landeta L. C. Renobales M. 1994 Hydrolysis of animal fats by immobilized Candida rugosa lipase. 16 61 65
Hita E. Robles A. Camacho B. Gonzalez P. A. Esteban L. Jimenez M. J. Munio M. M. Molina E. 2009 Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor 46 257 264
Jimenez M. J. Esteban L. Robles A. Hita E. Gonzalez P. A. Munio M. M. Molina E. 2010 Production of triacylglycerols rich in palmitic acid at sn-2 position by lipase-catalyzed acidolysis 51 172 179
Pilarek M. Szewczyk K. W. 2007 Kinetic model of 1,3-specific triacylglycerols alcoholysis catalyzed by lipases 127 736 744
Salis A. Sanjust E. Solinas V. Monduzzi M. 2003 Characterisation of Accurel MP1004 polypropylene powder and its use as a support for lipase immobilization. 24-25 75 82
Watanabe Y. Shimada Y. Sugihara A. Tominaga Y. 2002 Conversion of degummed soybean oil to biodiesel fuel with immobilized Candida antarctica lipase 17 151 155
Xie W. Ma N. 2010 Enzymatic transesterification of soybean oil by using immobilized lipase on magnetic nano-particles 34 890 896
Salis A. Svensson I. Monduzzi M. Solinas V. Adlercreutz P. 2003 The atypical lipase B from Candida antarctica is better adapted for organic media than the typical lipase from Thermomyces lanuginose. 1646 145 151
Palomo J. M. Fernandez-Lorente G. Mateo C. Ortiz C. Fernandez-Lafuente R. Guisan J. M. 2002 Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters. 31 775 783
Persson M. Mladenoska I. Wehtje E. Adlercreutz P. 2002 Preparation of lipases for use in organic solvents 31 833 841
Naoe K. Ohsa T. Kawagoe M. Imai M. 2001 Esterification by Rhizopus delemar lipase in organic solvent using sugar ester reverse micelles 9 67 72
Uehara A. Imai M. Suzuki I. 2008 The most favorable condition for lipid hydrolysis by Rhizopus delemar lipase in combination with a suger-ester and alcohol W/O microemulsion system. 324 79 85
Dizge N. Aydiner C. Imer D. Y. Bayramoglu M. Tanriseven A. Keskinler B. 2009 Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer. 100 1983 1991
Sabbani S. Hedenstrom E. Nordin O. 2006 The enantioselectivity of Candida rugosa lipase is influenced by the particle size of the immobilising support material Accurel 42 1 9
Zhou G. Chen Y. Yang S. 2009 Comparative studies on catalytic properties of immobilized Candida rugosa lipase in ordered mesoporous rod-like silica and vesicle-like silica 119 223 229
Dalla Rosa. C. Morandim M. B. Ninow J. L. Oliveira D. Treichel H. Vladimir Oliveira. J. 2009 Continuous lipase-catalyzed production of fatty acid ethyl esters from soybean oil in compressed fluids 100 5818 5826
Guan F. Peng P. Wang G. Yin T. Peng Q. Huang J. Guan G. Li Y. 2010 Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium 45 1677 1682
Ting W. J. Huang C. M. Giridhar N. Wu W. T. 2008 An enzymatic/acid-catalyzed hybrid process for biodiesel production from soybean oil. 39 203 210
Nagayama K. Yamasaki N. Imai M. 2002 Fatty acid Esterification catalyzed by Candida rugosa lipase in lecithin microemulsion-based organogels 12 231 236
Naya M. Imai M. 2012 Regulation of the hydrolysis reactivity of immobilized Candida rugosa lipase with the aid of a hydrophobic porous carrier 7 S1 S157 S165
Montero S. Blanco A. Virto M. D. Landeta L. C. Agud I. Solozabal R. Lascaray J. M. Renobales M. de Llama M. J. Serra J. L. 1993 Immobilization of Candida rugosa lipase and some properties of the immobilized enzyme. 15 239 247
Ahn K. W. Ye S. H. Chun W. H. Rah H. Kim S. G. 2011 Yield and component distribution of biodiesel by methanolysis of soybean oil with lipase-immobilized mesoporous silica 142 37 44
Huang D. Han S. Han Z. Lin Y. 2012 Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system 63 10 14
Khare S. K. Nakajima M. 2000 Immobilization of Rhizopus japonicas lipase on celite and its application for enrichment of docosahexaenoic acid in soybean oil. 68 153 157
Li S. F. Wu W. T. 2009 Lipase-immobilized electrospun PAN nanofibrous membranes for soybean oil hydrolysis 45 48 53
Li S. F. Fan Y. H. Hu R. F. Wu W. T. 2011 Pseudomonas cepacia lipase immobilized onto the electrospun PAN nanofibrous membranes for biodiesel production from soybean oil 72 40 45
Noureddini H. Gao X. Philkana R. S. 2005 Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil. 96 769 777
Ozmen E. Y. Yilmaz M. 2009 Pretreatment of Candida rugosa lipase with soybean oil before immobilization on β-cyclodextrin-based polymer. 69 58 62
Rodrigues R. C. Záchia Ayub. M. A. 2011 Effects of the combined use of Thermomyces lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil 46 682 688
Wang W. Li T. Ning Z. Wang Y. Yang B. Yang X. 2011 Production of extremely pure diacylglycerol from soybean oil by lipase-catalyzed glycerolysis 49 192 196
Xie W. Wang J. 2012 Immobilized lipase on magnetic chitosan microspheres for transesterification of soybean oil 36 373 380
Gitlesen T. Bauer M. 1997 Adlercreutz, P., Adsorption of lipase on polypropylene powder. 1345 188 196
Al-Duri B. Yong Y. P. 1997 Characterisation of the equilibrium behavior of lipase PS (from Pseudomonas) and lipolase 100L (from Humicola) onto Accurel EP100. 3 177 188
Stamatis H. Xenakis A. 1999 Biocatalysis using microemulsion-based polymer gels containing lipase 6 399 406