Example of possible preference indices, leaving, entering and net flow calculations and final ranking.Slightly modified after [5].
\r\n\tAccording to research, even in developed societies, at least three-quarters of the society has a health-related complaint, but only one-third of them consult a physician for help. Access to professional healthcare is low for several reasons. These reasons are poverty, lack of education or health literacy, lack of job skills, lack of a country's natural resources, lack of scientific knowledge, lack of infrastructures such as roads, communication lines, efficient government, false beliefs, bad environmental conditions such as pollution of water, soil, and air, racial, ethnic, sexual, and age discrimination in employment practices, corrupt or incompetent governments, poorly developed economy.
\r\n\tIn today's conditions, the increase in natural disasters due to global climate change and the loss of existing natural resources such as water and soil deteriorates good environmental conditions. Disasters cause acute health problems such as injuries and infections, and access to health services becomes difficult in disaster situations. For these reasons, professional health services should be well planned in crisis and disaster situations. Social justice and equity should be considered in planning. In recent years, we have seen an increase in violence and wars around the world. Wars caused by man-made disasters take away people's right to life and right to health. In addition, extraordinary situations such as wars, famine, floods, soil erosion, hurricanes, earthquakes, and droughts cause large migrations and make access to health services difficult. On the other hand, nuclear power plants threaten people's lives in some regions due to wars. It has also become difficult to provide health services for professional healthcare providers.
European legislation calls for a well-planned sustainable development. As such, it has to include a social, economic as well as an environmental dimension. According to Agenda 21 (http://www.un.org/esa/dsd/agenda21/), countries should undertake efforts to build up a comprehensive national inventory of their land resources in order to establish land information systems. The overall objective is to provide information for the improvement or the restructuring of land-use decision processes including the consideration of socio-economic and environmental issues.
\n\t\t\tIn the last decades conflicts caused by competing land uses have increased, particularly in urban areas. Consequently, a lot of research has been done aiming to develop methods and tools that assist complex spatial decision problems. The development of Spatial Decision Support Systems (SDSS) has turned out to be very beneficial in assisting to the solution of complex land-use problems [1, 3].
\n\t\t\tIn addition, any planning process must focus on a mix of hard (objective) and soft (subjective) information. The former are derived from reported facts, quantitative estimates, and systematic opinion surveys. The soft information denotes the opinions (preferences, priorities, judgments, etc.) of the interest groups and decision makers. The idea of combining the objective and subjective elements of the planning process in a computer based system lies at the core of the concept of SDSS [1, 3].
\n\t\t\tSDSS can be defined as an interactive, computer-based system designed to support a user or a group of users in achieving a greater degree of effectiveness in decision making when solving a semi-structured spatial decision problem [3]. SDSS also refers to the combination of GIS and sophisticated decision support methodologies, e.g. in terms of multicriteria analysis techniques [3, 6], and are therefore suitable to manage sustainable development of urban areas.
\n\t\t\tAlthough the development of multicriteria analysis began mainly in the \'70s (the first scientific meeting devoted entirely to decisionmaking was held in 1972 in South Carolina) its origins can be dated back to the eighteenth century [4]. Reflections on French policies in the action of judges and their translation into policy (social choice), led people like Condorcet to deepen in decision taken supported in several criteria [4].
\n\t\t\tIn the last two decades of the twentieth century there was an increased trend of integration of Multicriteria Evaluation techniques (MCE) and Geographic Information Systems (GIS), trying to solve some of the analytical shortcomings of GIS “For example see [4, 7, 15]“. Wallenius et al. [16], made a study of the evolution in the use of MCE techniques from 1992 to 2006, showing that the use of multiattribute techniques has increased 4.2 times during this period. In recent years, there has also been a great effort in the integration of MCE and GIS techniques on the Internet “For example see [17, 20]”.
\n\t\t\tSince we consider land-use decision making in general as an intrinsic multicriteria decision problem, in our opinion these are valid methodologies to support the land-use decision process by means of a land-use suitability analysis.
\n\t\t\tLand-use suitability analysis aims to identify the most appropriate spatial pattern for future land uses according to specified requirements or preferences [3, 21, 22]. GIS-based land-use suitability analyses have been applied in a wide variety of situations, including ecological and geological approaches, suitability for agricultural activities, environmental impact assessment, site selection for facilities, and regional planning [3, 6, 11, 17, 21,23, 28].
\n\t\t\tDifferent attempts to classify Multicriteria Decision Making (MCDM) methods by diverse authors exist in the literature [4, 6, 7, 11, 26, 29]. The majority of them agree that additive decision rules are the best known and most widely used Multiattribute Decision Making (MADM) methods in GIS based decision making. Some of the techniques more commonly described in literature are: Simple Additive Weighting (SAW), Ordered Weighting Averaging (OWA) technique, the Analytical Hierarchy Process (AHP), ideal point methods (e.g. TOPSIS), concordance methods or outranking techniques (e.g. PROMETHEE, Electre).
\n\t\t\tNevertheless, the integration of these techniques continues to pose certain problems or difficulties at the time of developing specific applications. Among the most notable drawbacks are [4]:
\n\t\t\tThe impracticality of applying pairwise comparison techniques as PROMETHEE with long series of data due to limitations posed by existing informatics systems.
The difficulty on the implementation of some MCE methods, thereby leading to a difficult analysis of the results, as well as an ignorance of the internal procedure of the methods by non-specialist users.
The need to generate data processing software attached to the GIS, based on algorithms that describe MCE methods, which naturally implies that many users of these systems cannot access these methods.
In this chapter, we compare the results obtained by the application of two distinctive land-use suitability analyses to the location of industrial sites, applying two different multicriteria analysis techniques. The multicriteria analysis employed has been performed in a raster environment and been used for two objectives. During the site search analysis each pixel was considered a potential location alternative. This analysis used a SAW method which signifies a weighted summation. It can thus easily be performed in GIS [24, 25, 30, 31]. A site selection analysis then used the PROMETHEE-2 methodology [32] and a set of predefined alternatives [30, 33]. All of the techniques used in the project were coded and integrated within ArcGIS by Marinoni [5, 34].
\n\t\t\tA problem in the application of multicriteria analysis is the definition of weights for a given set of criteria. A variety of approaches does exist, see for example [26], and the probably best known weight evaluation method is the AHP [35], which we have used in our case as well.
\n\t\t\tAnother problem is the specification of the criteria performance scores which are often subjective in their determination. Data which have been measured directly will certainly be regarded as more reliable than data which have been estimated, interpolated, taken from a map or simply interpreted. Thus, the method of criteria data collection plays a central role [5]. A stochastic approach which takes account of the uncertainty of input values and which is presented at a last step in this chapter could be a way out of this dilemma.
\n\t\tZaragoza city and its surroundings are located in the Ebro corridor, a highly dynamic economic area within the Iberian Peninsula. The climate in this area is semi-arid with mean annual precipitation of about 350 mm and a mean annual temperature of about 15° C.This city is crossed by the cited Ebro river and two of its main tributaries, the Gállego and Huerva rivers (Figure 1). Geologically, Quaternary alluvial terraces of the Ebro river were deposited above Tertiary gypsum formations, forming a covered karst area with intense karstification processes. The Quaternary materials are an important source of sand and gravel which are needed for civil engineering purposes. In addition, it hosts important groundwater reservoirs, used for domestic, industrial and agricultural purposes.
\n\t\t\t\t\n\t\t\t\tThe availability of these resources has been one of the reasons of the fast development of the city in the last decades. But this fast development has also led to negative interactions with the environment and man-made infrastructure. Intense irrigation triggered land subsidence which in turn caused costly damage and/or destruction of infrastructure such as roads, buildings, gas and water supply networks [36]. Many infrastructures that have been built occupy areas where soils of high fertility had naturally developed, making these areas inaccessible to agriculture. Also, many ecologically important areas have been harmed and an increased contamination of the aquifer has been observed [37].
\n\t\t\t\tLocation and geomorphology of study area.
Based on the above, the area surrounding Zaragoza, which represents a rapidly growing urban area, merits closer investigation in terms of geoscientific factors. Thus, a research project was initiated to develop a methodological workflow which will facilitate the sustainable development in the surroundings of a growing city. Our main objective was to perform a land-use suitability analysis to identify the most appropriate future land-use patterns. Therefore a variety of tasks needed to be performed such as:
\n\t\t\t\tCharacterization of the study area and collection, analysis and processing of the available information for its introduction into a GIS environment.
Geo-hazards and geo-resources detection, description and modelling with the help of GIS and 3D techniques.
Land-use suitability analysis by means of SDSS.
Here, we report on the land-use suitability analysis to find most suitable locations for industrial facilities. As mentioned above, we compare the results obtained by the application of two distinctive multicriteria analysis techniques for environmental decision making on industrial location. For more details on the general project workflow and geo-resources and geo-hazards modellingsee [24, 25, 30, 31, 33, 37, 39].
\n\t\t\tIt is important to differentiate between the site selection problem and the site search problem. The aim of site selection analysis is to identify the best location for a particular activity from a given set of potential (feasible) sites. Where there is no predetermined set of candidate sites, the problem is referred to as site search analysis [3].
\n\t\t\t\tIn terms of the MCE methods applied, the main advantage of the SAW approach can be considered its low degree of complexity as which made it attractive to be used for the site search analysis in this project. It is precisely this simplicity that makes weighted summation actually quite widely applied in real-world settings [8, 40, 42].
\n\t\t\t\tThe site selection analysis has been performed by the implementation of PROMETHEE-2 which belongs to the ‘family’ of outranking techniques. Since the mentioned techniques require pairwise or global comparisons among alternatives, these methods become impractical for applications where the number of alternatives ranges in the tens or hundreds of thousands (Pereira and Duckstein, 1993). For a more detailed description of both methodologies see [24, 25, 30, 33, 35, 36].
\n\t\t\t\tWorkflow of the land-use suitability analysis.
In order to perform both site search and site selection, several steps needed to be covered. These included (Figure 2):
\n\t\t\t\tDefinition of alternatives (decision options): feasible location areas.
Definition of constraints: areas with land-use restrictions.
Definition of important factors in the decision process: identification of criteria.
Determination of criteria weights
The criteria weights were determined with the AHP. This technique represents another MCE method and involves pairwise comparison of criteria where preferences between criteria are expressed on a numerical scale usually ranging from 1 (equal importance) to 9 (strongly more important). This preference information is used to compute the weights by means of an eigenvalue computation where the normalized eigenvector of the maximum eigenvalue characterizes the vector of weights. Empirical applications suggest that this pairwise comparison method is one of the most effective techniques for spatial decisionmaking approaches based on GIS [15, 43]. There exist many well-documented examples of application of this method with success [44, 46].
\n\t\t\t\tIt is well known that the input data to the GIS multicriteria evaluation procedures usually present the property of inaccuracy, imprecision, and ambiguity. In spite of this knowledge, the methods typically assume that the input data are precise and accurate. Some efforts have been made to deal with this problem by combining the GIS multicriteria procedures with sensitivity analysis [47] and error propagation analysis [48]. Another approach is to use methods based upon fuzzy logic [3].
\n\t\t\t\tIn many situations it is hard to choose the input values for multicriteria analysis procedures, since the criteria values for the different alternatives usually do not have a single realization, but can obtain a range of possible values [5]. Performing a multicriteria analysis with the mean values produces some kind of mean result, but the uncertainty in either the input values or the result cannot be quantified. A solution to this dead-end is a stochastic approach, which utilizes probability distributions for the input parameters instead of single values. A stochastic multicriteria analysis implies that the analysis is performed multiple times with varying input values for the criteria involved. These criteria input values (or performance scores) are drawn from probability distributions that are inferred from empirical criteria populations (e.g pixels on a map, expert knowledge). Such an approach uses the whole range of possible criteria value outcomes and extreme events are according to their low outcome probabilities realistically represented as rare events.\n\t\t\t\tIn a last step we explored the influence of criteria weightsby conducting a sensitivity analysis.
\n\t\t\t\tWithin the site search analysis, every pixel was considered a decision alternative. Constraints depict the areas where industry is and will not be allowed. These restrictions are generally characterized by the existence of other land uses (e.g. urbanareas), the protection of natural areas and land management planning. These restrictions are (Figure 3):
\n\t\t\t\t\tNatura 2000 network areas: natural reserve of the oxbows in La Cartuja (map provided by the Aragon Government).
Urbanized areas: obtained from the topographic map scale 1:25,000 from the National Geographical Institute (IGN,
Infrastructures (roads, rail roads, canals) and their area of protection: also extracted from the topographic maps. The area of protection of roads and train rails was delineated as defined by the Spanish Roads Law and according to the Spanish Railway Sector Law, respectively.
Other restrictive planning: Zaragoza Land Management Planning (PGOUZ,
Cattle tracks: tracks traditionally used by the seasonal migration of livestock which are protected by law, provided by the Aragon Government.
Industrial areas where no space is left for new industries. Provided by the Aragon Institute of Public Works (IAF,
Industrial restrictions.
A variety of social, economic and environmental factors were taken into consideration. Figure 4 shows the mapping of all the variables that were considered relevant for industrial development. Areas considered less suitable are kept in red while a higher suitability is shown in green.These variables are:
\n\t\t\t\t\tImportant areas from the environment point of view: natural areas included in the Natura network 2000 as SPAs (Special Protection Areas for birds), and the SACs (Special Conservation Areas), habitats, points of geological interest and other areas which mapping has been provided mainly by the Aragon Government.
Doline (sinkhole) susceptibility: model developed within the project using a quantitative method, a logistic regression technique [38].
Groundwater protection: a model developed also within the project, performed with Gocad [37] and applying a methodology by the German Geological Survey [49].
Flooding hazard: a flooding hazard mapping developed along the Ebro river [50] was digitised and introduced in the land-use suitability analysis. This model shows the different periods of return of flood events.
Agricultural capability of the soils: mapping developed within the project [39] applying the Cervatana Model [51].
Slope of the terrain: developed from the DEM (resolution 20x20 m) from the Ministry of Agriculture (
Geotechnical characteristics of the subsoil: different geomorphological units with better or worse geotechnical characteristics, described according to the PGOUZ. This classification has been applied to the geomorphological units derived from the geological map, scale 1:50,000, from National Geological Survey (IGME,
Variable mapping. 1) natural protected areas, 2) doline susceptibility, 3) groundwater protection, 4) flooding hazard, 5) agricultural capability of the soils, 6) slope percentage, 7) geotechnical characteristics.
Many multicriteria methods, as the SAW methodology, require criteria standardization to bring all of them to a common scale. The classification ensures that the weights properly reflect the importance of a criterion.The standardization method used here may be classified as a subjective scales approach [26] since the variables are classified in subjective ranges. These ranges can be selected following standards, legal requirements, or the classes already determined in the geo-resources and geo-hazards models used as criteria in the decision process. Six categories were selected considering the adaptation of these classes to the variables to be introduced. For more details on the standardization approach see [25, 30, 31].
\n\t\t\t\t\tWeights for criteria are assigned with the help of the AHP. An AHP extension was specifically developed for the ArcGIS environment at the Institute of Applied Geosciences of the Technische Universität Darmstadt [34]. This tool can be downloaded from the ESRI web page (http://arcscripts.esri.com/). For more details on the AHP performance see [25, 30, 31]. \n\t\t\t\t\tIn a last step all classified raster files (criteria) are multiplied by its corresponding weight and summed up.
\n\t\t\t\tThe main objective of a site selection analysis is the ranking of feasible alternatives. Generally, outranking methods, such as PROMETHEE-2, require pairwise or global comparisons among alternatives. Here location alternatives are represented by industrial areas, as defined in the Aragon Institute of Public Works (IAF) database, which signify spaces for the establishment of new industries. Geometrically, these alternatives represent a polygon each. A total of twenty seven industrial areas were evaluated for the site selection analysis.
\n\t\t\t\t\tAs alternatives are directly compared along their criteria values, the application of outranking methods does not require a transformation or standardization of criteria values. The restrictions (constraints) and criteria are the same used for the site search analysis. Alternatives located completely in restrictions areas were eliminated from the analysis. However, there exist some industrial polygons, representing one alternative, located partially in restricted areas, as these polygons are partially occupied or crossed by a road or a cattle track. It has implied the inclusion of the constraints as an additional criterion in the decision process. The criterion representative of use restrictions was then reclassified into two different values; zero in the area where industry is forbidden or not possible due to the presence of other uses, and one in areas where this use is permitted or feasible.
\n\t\t\t\t\tIt is important to define whether a higher value of a particular criterion leads to an improvement or to a decrease in land-use suitability. In the case of industrial development, an increase in the value of all criteria, with the exception of groundwater protection and geotechnical characteristics, implies a suitability decrease. For example, a higher groundwater protection value implies an increase in suitability to industrial use location while an increase in doline development susceptibility implies a decrease in industrial use location suitability.
\n\t\t\t\t\tGeometrically, every alternative is a polygon so that within each polygon a variety of criteria values (pixels in the criteria layers) are to be found. The question then arises which of the multiple criteria realization to use for the multicriteria analysis evaluation. Therefore, a multicriteria GIS extension was developed to draw site specific values (minimum, maximum, mean etc.) for raster cell populations that lie within the polygonal outline of a location alternative. For our analysis the mean value was used for all criteria since, in our opinion, this value better symbolizes all alternative values. Minimum and maximum values are usually rare events with a low probability of occurrence.
\n\t\t\t\t\tPROMETHEE-2 methodology uses preference function, which is a function of the difference between two alternatives for any criterion [32]. Six types of functions based on the notions of criteria, are proposed. For more details on preference functions see [5, 30, 32].
\n\t\t\t\t\tWe exclusively used the “usual criterion” preference function that is based on the simple difference of values between alternatives as this function helps to discriminate best between available alternatives which we wanted to achieve.
\n\t\t\t\t\tThe pair comparison of alternatives produces a preference matrix for each criterion (Figure 5). Having calculated the preference matrices along each criterion, a first aggregation is performed by multiplying each preference value by a weighting factor w (expressing the weight or importance of a criterion), and building the sum of these products [5]. This results in a preference index, Π (see Figure 5). The AHP has also been integrated in this tool and used for criteria weighting.
\n\t\t\t\t\tSchematic calculation of the preference index Π. Source [
The final ranking of alternatives is performed by calculating the net flow Φ (a1) for every alternative, a, which is a subtraction between the leaving flow and the entering flow. The higher the net flow is, the higher is the preference of an alternative over the others (Table 1).The leaving flow Φ+ (a1) represents a measure of the outranking character of a1 (how a1 is outranking all the other alternatives). Symmetrically, the entering flow Φ- (a1) is giving the outranked character of a1 (how a1 is dominated by all the other actions).
\n\t\t\t\tThe stochastic PROMETHEE-2 approach requires the assignment of theoretical distribution types to every criterion of the available alternatives. Distribution models were inferred based upon the criteria value populations (pixel values) within each location alternative (polygon) along all criteria. The software used to fit distribution types and to perform distribution fitting test was @Risk [52]. In a next step the distribution models were used within a Monte Carlo Simulation (MCS). The number of iterations
Starting a MCS with
\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t- | \n\t\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t\t0.75 | \n\t\t\t\t\t\t\t\t1.0 | \n\t\t\t\t\t\t\t\t0 | \n\t\t\t\t\t\t\t\t2 | \n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t0.75 | \n\t\t\t\t\t\t\t\t- | \n\t\t\t\t\t\t\t\t0.75 | \n\t\t\t\t\t\t\t\t1.5 | \n\t\t\t\t\t\t\t\t1 | \n\t\t\t\t\t\t\t\t1 | \n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t\t- | \n\t\t\t\t\t\t\t\t0.5 | \n\t\t\t\t\t\t\t\t-1 | \n\t\t\t\t\t\t\t\t3 | \n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t1 | \n\t\t\t\t\t\t\t\t0.5 | \n\t\t\t\t\t\t\t\t1.5 | \n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t |
Example of possible preference indices, leaving, entering and net flow calculations and final ranking.Slightly modified after [5].
However, the alternative possessing the highestnumber of first ranks may not necessarily be the best [5]. Therefore, it was suggested calculating a dimensionless mean stochastic rank MSR for every alternative.
\n\t\t\t\t\twhere:
\n\t\t\t\t\tm: number of iterations
\n\t\t\t\t\tAj: jth alternative
\n\t\t\t\t\tn: number of available alternatives
\n\t\t\t\t\tRi: rank count for the ith rank
\n\t\t\t\t\tIn order to compare mean stochastic ranks of simulations with different iteration counts, the MSR value must be standardized which leads to the stochastic rank index SI [5]:
\n\t\t\t\t\twhere:
\n\t\t\t\t\tm: number of iterations
\n\t\t\t\t\tSIAj: stochastic rank index for the jth alternative
\n\t\t\t\t\tMSRAj: MSR for the jth alternative
\n\t\t\t\t\tMSRmin: the lowest possible MSR value
\n\t\t\t\t\tMSRmax: the largest possible MSR value
\n\t\t\t\t\tThe more the SI value approaches 0, the better the alternative.
\n\t\t\t\t\tPROMETHEE input value determination for one iteration cycle. Source: [
Left: example distribution of 4 scenarios (s1,..., s4) for rank 1. Right: rank distribution for scenario 1. Source: [
The criteria preference values have been assigned by the authors after discussions with experts from different stakeholder groups from the Zaragoza City Council and the Ebro River Authority (CHE,
The validation of a model consists in checking whether the structure of the model is suitable for the purpose and if it achieves an acceptable level of accuracy in predictions. In the case of explanatory or predictive models, validation is usually carried out by checking the degree of agreement between the data produced by the model and data from the real world [4]. In the case of our project in order to validate the model has been verified that the result follows the preferences in the assignation of the weights to the criteria.
\n\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t8.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t0.2288 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t0.1736 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.1131 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t8.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t0.2288 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.0678 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.13 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.13 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.0251 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.0427 | \n\t\t\t\t\t\t
Pairwise comparison matrix, criteria weights for site searchanalysis.A) Groundwater protection, B) Doline susceptibility, C)Flooding hazard, D)Location of natural areas, E) Agricultural capability of soils, F) Slope percentage, G) Geotechnical characteristics.
\n\t\t\t\t\tFigure 8 shows the final results of the land-use suitability analysis for new industrial development. The lefthand side of figure 8 shows the suitability map under sustainability. The grey sections indicate the areas where industrial locationis not possible due to the constraints. Although the suitability analysis sometimes presents good values, the constraints imply that these areas cannot be exploited due to any restriction.The most suitable locations for industrial development are on the pediments or glacis (Figure 1) and Tertiary materials outside environmental protected areas where the groundwater vulnerability and flood risk is lower. The least suitable locations are the floodplains with high groundwater vulnerability and flood risk, environmentally protected areas around the river bed and other areas in the higher terraces which present more susceptibility to doline development.
\n\t\t\t\tTo test the robustness of the results, a sensitivity analysis of the model has been performed where higher weights were given to economic aspects. The highest weights were assigned to doline susceptibility and flooding hazard, which might cause the destruction of future industrial sites (Table 3). Slope and geotechnical characteristics of the soils were also assigned high values as a more technically difficult terrain will increase the construction budget. Figure 8 shows the results of this last approach where the best locations for industry were identified to be the pediments and slopes in Tertiary sediments. The least favorable locations are on the flood plain and low river terraces, where sinkhole susceptibility shows higher values. In fact, in order to measure the correlation between both results the Pearson coefficient of correlation between both raster images has been calculated giving a value of 0.874, significant at a 0.01 level, implying a high agreement between both results.
\n\t\t\tResult of a) site search analysis under sustainability and b) sensitivity analysis for industrial development site search analysis (objective economic development).
As a consequence of the introduction of a new criterion depicting restriction of use or constraints as explained in section 2.2.2., the criteria weights used for the site search analysis are not valid implying a new calculation of them using the AHP. Table 4 shows the preference matrix and criteria weights of the site selection analysis for industrial development. The criteria preference values are the same as for the site search analysis (Table 2) under the sustainability scenario, however the constraints obtained the highest preference values and as a consequence the highest weight, in order to avoid the outranking of alternatives located partially in forbidden areas.
\n\t\t\t\t\n\t\t\t\tThe preference indices and the leaving and entering flow generated after the application of PROMETHEE-2 methodology are presented in Table 5. Figure 9 shows the location of the alternatives of the site selection analysis. The best alternatives are generally located south of Zaragoza city, outside the alluvial sector (i.e. alternatives 25, 26 and 27). In contrast, the worst locations are the alluvial areas in the surroundings of El Burgo de Ebro (i.e. alternatives 4 and 18), the industrial areas in the north of Zaragoza city (i.e. alternatives 16 and 17), and the Logroño Road Corridor, upstream of Zaragoza (i.e. alternative 8).
\n\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.0735 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t8.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.3492 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.1774 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.0505 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t0.13 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.0224 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.1892 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.1378 | \n\t\t\t\t\t\t
Pairwise comparison matrix, criteria weights for site search analysis under economic aspects.A) Groundwater protection, B) Doline susceptibility, C)Flooding hazard, D)Location of natural areas, E) Agricultural capability of soils, F) Slope percentage, G) Geotechnical characteristics.
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t8.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.197 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.121 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.087 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t5.00 | \n\t\t\t\t\t\t\t8.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.197 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.048 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.13 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.13 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t0.11 | \n\t\t\t\t\t\t\t0.020 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.25 | \n\t\t\t\t\t\t\t0.20 | \n\t\t\t\t\t\t\t0.17 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.14 | \n\t\t\t\t\t\t\t0.030 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t3.00 | \n\t\t\t\t\t\t\t4.00 | \n\t\t\t\t\t\t\t2.00 | \n\t\t\t\t\t\t\t6.00 | \n\t\t\t\t\t\t\t9.00 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t1.00 | \n\t\t\t\t\t\t\t0.300 | \n\t\t\t\t\t\t
Pairwise comparison matrix, criteria weights for site selection analysis.A) Groundwater protection, B) Doline susceptibility, C)Flooding hazard, D)Location of natural areas, E) Agricultural capability of soils, F) Slope percentage, G) Geotechnical characteristics, H) Constraints.
In the stochastic approach, distribution types have to be assigned to every alternative and criterion and a MCS is performed over a MCE method (here PROMETHEE-2) meaning that the multicriteria analysis is performed a specified number of times (here: 5000; hence stochastic PROMETHEE-2). It should be noted that, due to local/regional variability, the local distributions of a criterion are highly likely to be different for each location alternative. Thus, although it seems reasonable, at first sight, to determine one distribution type for one criterion, if location dependent statistical analyses indicate varying distribution types, then varying types should be assigned to one criterion.
\n\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tSAW Mean value | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.69 | \n\t\t\t\t\t\t\t12.22 | \n\t\t\t\t\t\t\t-1.48 | \n\t\t\t\t\t\t\t14 | \n\t\t\t\t\t\t\t0.41 | \n\t\t\t\t\t\t\t10 | \n\t\t\t\t\t\t\t3.95 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.41 | \n\t\t\t\t\t\t\t8.74 | \n\t\t\t\t\t\t\t-4.67 | \n\t\t\t\t\t\t\t20 | \n\t\t\t\t\t\t\t0.75 | \n\t\t\t\t\t\t\t22 | \n\t\t\t\t\t\t\t3.09 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.65 | \n\t\t\t\t\t\t\t8.62 | \n\t\t\t\t\t\t\t-5.03 | \n\t\t\t\t\t\t\t22 | \n\t\t\t\t\t\t\t0.65 | \n\t\t\t\t\t\t\t19 | \n\t\t\t\t\t\t\t3.26 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t15.76 | \n\t\t\t\t\t\t\t8.50 | \n\t\t\t\t\t\t\t-7.25 | \n\t\t\t\t\t\t\t25 | \n\t\t\t\t\t\t\t0.50 | \n\t\t\t\t\t\t\t13 | \n\t\t\t\t\t\t\t3.95 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t12.77 | \n\t\t\t\t\t\t\t9.28 | \n\t\t\t\t\t\t\t-3.49 | \n\t\t\t\t\t\t\t16 | \n\t\t\t\t\t\t\t0.70 | \n\t\t\t\t\t\t\t21 | \n\t\t\t\t\t\t\t3.25 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t7.48 | \n\t\t\t\t\t\t\t12.93 | \n\t\t\t\t\t\t\t5.45 | \n\t\t\t\t\t\t\t8 | \n\t\t\t\t\t\t\t0.27 | \n\t\t\t\t\t\t\t7 | \n\t\t\t\t\t\t\t4.60 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t6.42 | \n\t\t\t\t\t\t\t17.08 | \n\t\t\t\t\t\t\t10.66 | \n\t\t\t\t\t\t\t4 | \n\t\t\t\t\t\t\t0.06 | \n\t\t\t\t\t\t\t1 | \n\t\t\t\t\t\t\t5.38 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.31 | \n\t\t\t\t\t\t\t6.91 | \n\t\t\t\t\t\t\t-6.40 | \n\t\t\t\t\t\t\t24 | \n\t\t\t\t\t\t\t0.69 | \n\t\t\t\t\t\t\t20 | \n\t\t\t\t\t\t\t3.55 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t11.97 | \n\t\t\t\t\t\t\t8.25 | \n\t\t\t\t\t\t\t-3.72 | \n\t\t\t\t\t\t\t17 | \n\t\t\t\t\t\t\t0.56 | \n\t\t\t\t\t\t\t14 | \n\t\t\t\t\t\t\t3.75 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t12.30 | \n\t\t\t\t\t\t\t8.11 | \n\t\t\t\t\t\t\t-4.18 | \n\t\t\t\t\t\t\t18 | \n\t\t\t\t\t\t\t0.63 | \n\t\t\t\t\t\t\t17 | \n\t\t\t\t\t\t\t3.32 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t9.49 | \n\t\t\t\t\t\t\t10.92 | \n\t\t\t\t\t\t\t1.44 | \n\t\t\t\t\t\t\t10 | \n\t\t\t\t\t\t\t0.42 | \n\t\t\t\t\t\t\t11 | \n\t\t\t\t\t\t\t3.69 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.30 | \n\t\t\t\t\t\t\t8.96 | \n\t\t\t\t\t\t\t-4.34 | \n\t\t\t\t\t\t\t19 | \n\t\t\t\t\t\t\t0.63 | \n\t\t\t\t\t\t\t18 | \n\t\t\t\t\t\t\t3.79 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t8.99 | \n\t\t\t\t\t\t\t11.42 | \n\t\t\t\t\t\t\t2.44 | \n\t\t\t\t\t\t\t9 | \n\t\t\t\t\t\t\t0.56 | \n\t\t\t\t\t\t\t15 | \n\t\t\t\t\t\t\t3.57 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t8.61 | \n\t\t\t\t\t\t\t15.29 | \n\t\t\t\t\t\t\t6.69 | \n\t\t\t\t\t\t\t7 | \n\t\t\t\t\t\t\t0.19 | \n\t\t\t\t\t\t\t5 | \n\t\t\t\t\t\t\t4.66 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t10.74 | \n\t\t\t\t\t\t\t9.97 | \n\t\t\t\t\t\t\t-0.77 | \n\t\t\t\t\t\t\t12 | \n\t\t\t\t\t\t\t0.60 | \n\t\t\t\t\t\t\t16 | \n\t\t\t\t\t\t\t4.76 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t15.17 | \n\t\t\t\t\t\t\t4.93 | \n\t\t\t\t\t\t\t-10.25 | \n\t\t\t\t\t\t\t26 | \n\t\t\t\t\t\t\t0.95 | \n\t\t\t\t\t\t\t27 | \n\t\t\t\t\t\t\t3.61 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t13.40 | \n\t\t\t\t\t\t\t7.00 | \n\t\t\t\t\t\t\t-6.40 | \n\t\t\t\t\t\t\t23 | \n\t\t\t\t\t\t\t0.88 | \n\t\t\t\t\t\t\t26 | \n\t\t\t\t\t\t\t3.75 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t19.37 | \n\t\t\t\t\t\t\t6.63 | \n\t\t\t\t\t\t\t-12.75 | \n\t\t\t\t\t\t\t27 | \n\t\t\t\t\t\t\t0.86 | \n\t\t\t\t\t\t\t25 | \n\t\t\t\t\t\t\t2.55 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t11.97 | \n\t\t\t\t\t\t\t8.74 | \n\t\t\t\t\t\t\t-3.24 | \n\t\t\t\t\t\t\t15 | \n\t\t\t\t\t\t\t0.45 | \n\t\t\t\t\t\t\t12 | \n\t\t\t\t\t\t\t4.32 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t12.74 | \n\t\t\t\t\t\t\t7.78 | \n\t\t\t\t\t\t\t-4.96 | \n\t\t\t\t\t\t\t21 | \n\t\t\t\t\t\t\t0.76 | \n\t\t\t\t\t\t\t24 | \n\t\t\t\t\t\t\t3.9 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t10.83 | \n\t\t\t\t\t\t\t9.58 | \n\t\t\t\t\t\t\t-1.25 | \n\t\t\t\t\t\t\t13 | \n\t\t\t\t\t\t\t0.76 | \n\t\t\t\t\t\t\t23 | \n\t\t\t\t\t\t\t3.83 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t5.25 | \n\t\t\t\t\t\t\t15.16 | \n\t\t\t\t\t\t\t9.91 | \n\t\t\t\t\t\t\t5 | \n\t\t\t\t\t\t\t0.33 | \n\t\t\t\t\t\t\t8 | \n\t\t\t\t\t\t\t4.68 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t4.90 | \n\t\t\t\t\t\t\t15.82 | \n\t\t\t\t\t\t\t10.92 | \n\t\t\t\t\t\t\t3 | \n\t\t\t\t\t\t\t0.21 | \n\t\t\t\t\t\t\t6 | \n\t\t\t\t\t\t\t5.68 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t10.13 | \n\t\t\t\t\t\t\t9.98 | \n\t\t\t\t\t\t\t-0.15 | \n\t\t\t\t\t\t\t11 | \n\t\t\t\t\t\t\t0.39 | \n\t\t\t\t\t\t\t9 | \n\t\t\t\t\t\t\t5.61 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t5.78 | \n\t\t\t\t\t\t\t17.63 | \n\t\t\t\t\t\t\t11.85 | \n\t\t\t\t\t\t\t1 | \n\t\t\t\t\t\t\t0.11 | \n\t\t\t\t\t\t\t4 | \n\t\t\t\t\t\t\t4.88 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t6.18 | \n\t\t\t\t\t\t\t17.32 | \n\t\t\t\t\t\t\t11.14 | \n\t\t\t\t\t\t\t2 | \n\t\t\t\t\t\t\t0.10 | \n\t\t\t\t\t\t\t3 | \n\t\t\t\t\t\t\t4.80 | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t7.22 | \n\t\t\t\t\t\t\t17.04 | \n\t\t\t\t\t\t\t9.83 | \n\t\t\t\t\t\t\t6 | \n\t\t\t\t\t\t\t0.07 | \n\t\t\t\t\t\t\t2 | \n\t\t\t\t\t\t\t5.32 | \n\t\t\t\t\t\t
Leaving floe, entering flow, net flow and rank for site selection analysis, stochastic rank index and final rank for stochastic approach, and mean value in SAW methodology for every alternative of location.
\n\t\t\t\t\tTable 6 show the distribution types assigned to every alternative and criterion for the suitability analyses. Distribution fitting tests were performed to confirm/reject a modeled distribution type. The software used was @Risk [52]. If physical properties can only have non-negative values distribution types can (and should) be selected such that this feature is reflected, for example by choosing an exponential distribution.
\n\t\t\t\tLocation of alternatives for site selection analysis.
The more commonly used distributions for continuous variables (i.e. slope percentage) are normal and lognormal, but also logistic and exponential distributions are present in some variable (i.e. groundwater protection and/or doline susceptibility).
\n\t\t\t\tA binomial distribution was selected for categorical variables having two possible outcomes.If there are more than two categories (possible outcomes) the use of a categorical distribution can be problematic, implying the inclusion in the decision process of categories not present in the alternative. For example, if one alternative presented values 1 and 4 in agricultural capability criterion, the distribution selected by the fitting test would have given values 2 and 3 to this alternative, which are not present in the real world. Thus, instead of assigning a distribution, the percentage of cases (p value in Table 6) in every category was calculated and used as the probability of occurrence of every category. This was also the case for some continuous variables, which presented few different values, thus complicating the distribution selection (i.e. alternative 3 in doline susceptibility criterion). In these cases, the percentage or probability of occurrence of every value was introduced in the analysis.
\n\t\t\t\tIn the case of the criterion “susceptibility to doline development”, difficulties were experienced as some alternatives showed continuous values close to value 0 (see Figure 10). Since it was not possible to apply the percentage of values in these cases, a decision was made to apply an exponential distribution in order to avoid the introduction of negative values in the suitability analysis, even though the adopted solution was not absolutely satisfactory. Finally, some alternatives presented the same value for the whole alternative (unique value in the tables). Some representative examples of the selected distribution types can be seen in Figures 10. The bars symbolize the original (empirical) values retrieved from the pixels within each location alternative; the solid line the fitted theoretical distribution model.
\n\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tiu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tl | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tn | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\te | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tu | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tln | \n\t\t\t\t\t\t\tp | \n\t\t\t\t\t\t\tb | \n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t\t | \n\t\t\t\t\t\t |
Distribution types for every alternative and criterion for industrial settlements suitability analysis. Al.) Alternative, A) Groundwater protection, B) Doline susceptibility, C) Flooding hazard, D) Location of natural areas, E) Agricultural capability of soils, F) Slope percentage, G) Geotechnical characteristics, H) Constraints. p) percentage, ln) lognormal, u) unique value, b) binomial, l) logistic, n) normal, e)exponential, iu) Intuniform
The results of the site selection suitability analysis based on stochastic PROMETHEE-2 can be seen in Table 5. In general, there are few differences in the SI values and total flows between the first rankings: alternatives 7, 25, 23, 26 and 27 (Figure 9). In addition, all these alternatives are located in the areas with higher suitability values in the site search analysis (SAW mean value in Table 5). Nevertheless, alternative 24 presents a high mean value in the SAW methodology but is ranked 11 and 9 in the PROMETHEE-2 and the stochastic approach. This is due to the fact that the major part of this polygon is located in a restricted area. In fact, the worst rankings, alternative 16, 17 and 18, are located partially inside restricted areas. However, in the case of alternative 24 the weight assigned to the constraint factor in PROMETHEE-2 and the stochastic approach it was not enough to rank this alternative in the last positions. Besides, the first rank changes from alternative number 25 to alternative number 7, in the PROMETHEE-2 and the stochastic approach, respectively. This is the consequence of assigning a unique mean value in the PROMETHEE-2 approach to the alternatives. Figure 11 saws the values of the SAW methodology for both alternatives. It can be observed how, although both alternatives present similar mean value, alternative 7 present homogeneous high values, implying more percentage of high values in its distribution, while alternative 25 present a variety of suitability values, implying a less percentage of high suitability values. The stochastic approach overcomes this handicap by simulating values along the whole range of values inside the distribution assigned to the alternatives.
\n\t\t\t\tExamples of distributions assigned to alternatives.
The industrial use suitability map developed with the SAW and AHP methods integrated in a GIS for the surroundings of Zaragoza, is a substantial aid in the land-use management of this city. Besides, an additional benefit is achieved by integrating geoscientific aspects in the land-use decision process, as demanded by Agenda 21.
\n\t\t\tSAW values for alternatives 7 and 25.
A fundamental problem of decision theory is how to derive weights of criteria. One disadvantage of the AHP method is the inherent subjectivity of assigning preference values between criteria. The weights derived from these preference values have usually a profound effect on the results of the suitability analysis. However, in our particular case, in the industrial suitability analysis, there were no strong differences between the results of the site search analysis performed under the concept of sustainable development or the site search analysis performed under the concept of economic development, although different weights were assigned to the criteria in both approaches.
\n\t\t\tIf differences are greater, a possible solution is to establish a set of suitability maps and to combine these to select the most suitable areas.
\n\t\t\tAfter some talks with different managers in the administration and following the approach under sustainability aspects, our results suggest that the best location for new industries is on the pediments and Tertiary sediments outside the natural protected areas, where the groundwater vulnerability and flood risk is lower, although the geotechnical characteristics of the terrain are less favorable, according to the PGOUZ. The least favorable location embodies the floodplain with high groundwater vulnerability values and the natural protected areas around the river bed, and other areas in the higher terraces which are more susceptible to doline development.
\n\t\t\tAn advantage of outranking methods as PROMETHEE-2 is the fact that criteria do not need standardization or transformation processes which reduces subjectivity. However, in a spatial multicriteria analysis decisions still need to be made, as for example what characteristic value (from the population of pixels within a location alternative polygon) to use for a subsequent multicriteria analysis (e.g.maximum, minimum, mean, etc.). If using PROMETHEE-2 more decisions needs to be made in regards to the selection of the preference function as well as which set of criteria weights to use.
\n\t\t\tIt is important to notice the similarity of the results after applying the site search analysis and the site selection analysis. In general, the highest rank positions are present in alternatives located in areas where the site search analysis also presented the highest suitability values. Some differences can be observed in alternatives located in areas with restrictions, as in the site selection analysis constrains are included as criteria. This is the case of alternative 24 which presented a high mean value in the SAW methodology but present a rank 11 and 9 in the PROMETHEE-2 and the stochastic approach. In this case, the weight assigned to the constraint factor in PROMETHEE-2 and the stochastic approach it was not enough to rank this alternative in the last positions. Thus, a higher weight should be given to the constraint factor in the site selection approaches.
\n\t\t\tPerforming a PROMETHEE-2 with the mean values produces a mean result, but the uncertainty in either the input values or the result cannot be quantified. The stochastic approach helps approaching this problem by using probability distributions for the input parameters, instead of single values. For spatial multicriteria analysis in a variable data environment it is our recommendation to use stochastic approaches although, in this case, the process was not absolutely integrated in the GIS, and as a consequence it is very time consuming.
\n\t\tThis research was funded by the Deutsche Forschungsgemeinschaft (DFG, Ho 804/7-1+2). We also greatly acknowledge support of the Ebro River Authority (CHE), the Aragón Region Authority and the Zaragoza Council.
\n\t\tWith the dawn of the age of nanotechnology, there has been an intense scurrying and scavenging for nanomaterials with unique properties and specific molecular arrangements that allow it to find application in specific niches inaccessible to alternative forms. Nanostructured materials display unique physicochemical properties such as excellent electrical and thermal conductivity, solubility, porosity, surface interactions, density, band gap and surface electronic charge resulting in exceptional catalytic and optical activity and enhanced performance compared to their bulk counterparts [1]. Presently, nanoscale devices have widespread application in cell targeted therapeutic delivery, high resolution tissue imaging and in replacing damaged tissue [2]. In agriculture, nanomaterials are being used to enhance crop production as nanofertilizers [3] and for crop protection as nanopesticides and nanobiosensors [4]. These active ingredients are encapsulated in nanocapsules, micelles, gels, liposomes, mesoporous silica nanoparticles or hollow nanoparticles to ensure controlled release, better solubility and for active stablity in the long-term [5]. To compensate for hazardous emissions to the environment, nanomaterials have been functionalized to remove contaminants through adsorption [6], immobilization, photocatalytic degradation, and electro-nanoremediation [7]. It is therefore undeniable that uncovering novel multifunctional nanosized materials is an elaborate pursuit with promising outcomes, yet filled with pressing concerns which are in dire need to be addressed.
One of the primary concerns of nanotechnology is the indiscriminate release of hazardous nanowaste, generated during the manufacturing and processing of engineered of nanomaterials, which could inevitably accumulate in the environment and inevitably end up in the food chain [8]. This has roused an overdrive in the hunt for sustainable nanomaterials from renewable bioresources such as cellulose, starch, chitosan, gelatin, alginate and chitin which are biodegradable, leave minimal implications on health and the environment and could be retrieved as value added waste in the production of a new generation of green nanomaterials [9].
Cellulose is a renewable feedstock with interesting properties such as biocompatibility and biodegradability. It is found to be chemically inert, displays excellent stiffness, high strength and dimensional stability, low density and easily functionalized surface chemistry [10]. Lignocellulosic biomass such as wood and agricultural residues such as tree trunks, rice straw, sugarcane bagasse, coconut husks, oil palm empty fruit bunches energy crops and grass are excellent feedstocks for green nanomaterials derived from cellulose or nanocellulose. This natural biopolymer is abundantly available and can be used as renewable feedstock in the generation of sustainable nanomaterials [11]. Reconstruction of lignocellulosic biomass waste residues into value added products such as nanostructures is an attractive, feasible option [12].
Cotton is an abundantly available fibrous crop grown for global commercial production with over 95% cellulose in its plant structure. Cotton stalk which is an overbearing agricultural residue generated in cotton-producing countries such as India, USA, China, Brazil and Pakistan, represents a semi-wood raw material made up of cellulose, hemicellulose, and lignin which could be utilized to fabricate value-added nanocellulose, paving an excellent way to maximize the utilization of waste [13]. Nanocellulose has exceptional properties such as high tensile strength, high Young’s modulus, low weight, mechanical robustness, low coefficient of thermal expansion, biodegradability, surface functionality and hydrophilicity, biocompatibility and lack of toxicity [14]. In recent times, nanocellulose is used in energy storage, as aerogels, emulsion stabilizers, enzyme immobilization substrates, low-calorie food additives, reinforcing fillers, pharmaceutical binder, biomimetic materials and biosensors [15, 16, 17]. Nanocellulose derived from cotton feedstock can be broadly categorized as cellulose nanocrystals (CNC) and nanofibrillated cellulose (NFC). CNCs (as shown in Figure 1), also known as cellulose nanowhiskers or nanorods, are short (<500 nm) and narrow (<40 nm) rod shaped, rigid crystalline structures with diameters between 1 and 100 nm [18] with tremendous application potential in regenerative medicine [19], optoelectronics [20], automotive polymers [21] and as composite materials [22]. It is generated by eliminating the amorphous regions in cellulose fibers using acid hydrolysis [23]. CNC have been extracted from cotton fibers [24], processed cotton [25] and cotton linters [26], a byproduct of cotton processing. NFC or cellulose nanofibers (as shown in Figure 2) are longer (< 3000 nm) and wider (< 100 nm) fibers with low crystallinity obtained by the mechanical disintegration of cotton biomasses using a high-speed ball grinder [27], ultrasonicator [28] or high-pressure homogenization [29].
TEM image depicting cotton-derived CNC.
FESEM images of the cotton based NCFs.
Nanocomposites are materials made up of 2 or more constituent phases with at least 1 phase of nano-size particles (<100 nm) which creates a discontinuous phase over a matrix of standard material [30]. This unique multiphase structure that is reinforced by a stronger component of nanosized fillers [31] demonstrates greater mechanical and tensile strength and increased capacity for thermal expansion and conductivity [32]. CNCs are interesting materials that could function as nanofillers owing to the abundance of the -OH groups, reactivity, high surface area, mechanical, thermal and optical properties, even at low concentrations [33] which enhances tensile strength and decreases elasticity due to the strong intermolecular linkages such as covalent bonds, van der Waals forces, mechanical interlocking and molecular entanglement between the fillers and its polymeric matrix [34]. Various methods have been developed to generate cellulose nanocomposites which include melt extrusion, ball milling, injection molding, compression molding, 3D printing, layered assembly, electrospinning, among others [35, 36]. Cellulose nanocomposites find vast application as packaging material, automotive and aerospace paints and coatings, adhesives, hydrogels, nanobarriers, fire retardants, construction materials, military defense and as emerging smart hybrids which display outstanding properties such as stretch ability, high mechanical strength, optical transparency, electrical and thermal conductivity, porosity and high adsorption [37]. Cotton based cellulose nanocomposites constructed with metals, metal oxides and non-metallic elements have exhibited innovative features due to its synergetic effects which are unattainable as pure nanomaterials [38]. Nanocomposites loaded with noble metal nanostructures have antibacterial properties and are used in biomedicine, enzyme immobilization, catalysis and as biosensors [39]. Rumi et al., 2021 observed that cotton-based CNC display high crystallinity, tensile strength and stiffness making it an attractive engineering nanomaterial for composite reinforcement [40]. In a separate study, Araujo et al., 2018 found that biopolymer nanocomposites reinforced with hydrolyzed cotton NFC extracted from cotton waste textiles resulted in a composite material with greater tensile strength and thermal capacity compared to the pure biopolymer [25]. Rafaella et al., 2019 constructed a cotton NFC/chitosan nanocomposite with collagen like properties which demonstrated increased surface roughness, improved cell adhesion, spreading and proliferation when used as scaffolds in tissue engineering [41]. Thus, surface modification of polymeric materials with cotton NFC for substrates used as scaffolds in tissue engineering would result in functionalized nanocomposites with novel physicochemical properties and large surface area which allow numerous contact points between cells and the nanocomposite surfaces for cell viability and growth. In a separate study, Li et al., (2013) generated cotton CNC through electrospinning and functionalized it into composites by surface coating it with CeO2 nanoparticles using the hydrothermal reaction. The resulting cotton based cellulose nanocomposite demonstrated excellent UV-shielding and enhanced photocatalytic properties making it of great value in medicine, military operations and optoelectronics [42].
Multifunctional cotton-based nanomaterials have been inadvertently thrust into the limelight with the recent Covid-19 pandemic through the design of various nanosensor devices for viral detection, surface decontaminants, antiviral compounds and nanocomposite fabrics which serve to prevent or annihilate the SARS-CoV-2. In this aspect, cotton nanocomposites have been constructed as nanosensors in the detection of the virus and as antimicrobial textiles for medical PPE (personal protective equipment). Eissa and Zourob, (2021) fabricated a cotton CNF-tipped electrochemical immunosensor as a one-step diagnostic tool for the detection of SARS-CoV-2 viral antigen [43]. Textiles embedded with antimicrobial nanoparticles such as Ag, ZnO and CuO have been tailored as a protective measure in PPE’s for those on the frontline of defense against the SARS-CoV-2. An extensive research resulting in the design and manufacture of antibacterial cotton-based face mask embedded with CuO nanoparticles (CuONps) demonstrated that cotton could be reconstructed as an antimicrobial nanocomposite and used as a PPV fabric to secure the protection of medical personnel embodying it [37]. In this work, Perelshtein et al., 2016 functionalized cotton fabric with CuONps using ultrasound-assisted deposition by an in-situ coating process on the surface of the fabric. The resulting nanocomposite material retained excellent antibacterial properties after 65 washing cycles at 75–92°C, making it an excellent material as a reusable medical PPE [44]. In a separate study, Adhikari et al., 2021 synthesized a nanoceutical cotton ZnO composite fabric using the hydrothermal method to filter viral particles without compromising on user’s breathing mechanism [45]. The design of this nanoceutical fabric was constructed to find application as a one-way valve in a face mask that would facilitate breathing while trapping and filtering airborne viral pathogens and reducing transmission through droplets. It is therefore undisputable that cotton nanocomposite fabrics are the textiles of the future as a shield of protection in the war against the multitude of rising murderous pathogens of this millennia.
Cotton textiles are used widely in numerous applications and various industries particularly as sportswear and medical textiles due to its exceptional properties such as breathability, hypo allergenicity, hygroscopicity and low cost [46]. Some of the drawbacks of cotton include low tensile strength, UV-vulnerability, enhanced capacity for microbial growth and easily wrinkled [47]. Inserting nanoparticles into cotton as antimicrobial agents to form nanocomposites is a way forward to manufacture value-added fabric material [48]. These nanocomposites which are formed through the in situ or ex situ deposition of nanoparticles in the fabric material has endowed multi-functionalities to the cotton fabrics such as self-cleaning, UV protection and electric conductivity [49]. Cotton based textiles can actually be designed with self-cleaning features when hydrophobic surfaces are fabricated on these textiles to repel water in such a way that spherical droplets of water can remove stains through a mechanism known as easy roll-off. Wu et al., 2016 demonstrated that a sequential deposition of poly(ethylenimine), silver nanoparticles (AgNp) and fluorinated decylpolyhedral oligomeric silsesquioxane (F-POSS) on cotton fabrics resulted in a superhydrophobic surface entailing a 169° angle of water contact with a 3° sliding angle [50]. Cotton based nanocomposites embedded with ZnO, TiO2 and reduced graphene oxides have also shown great promise in UV protection [51] and electromagnetic interference (EMI) shielding properties [52].
Fabrics with antimicrobial properties are sought after for the manufacture of healthcare textiles particularly as packaging material for drugs and syringes or medical tools, for the personal protective gear of medical personnel, in wound dressing, surgical aprons and hospital bedding [53]. While cotton is undoubtedly widely popular in the textile industry, its fibers are highly hydrophilic with a high tendency of water absorption and oxygen retention and with a large surface area causing it to be a breeding ground for bacteria and fungi [54]. Cotton nanocomposites have been designed to incorporate metallic nanoparticles for the demonstration of antimicrobial activity [55]. Incorporation of antimicrobial metallic nanoparticles into cotton to generate nanocomposites could be carried out via ex situ or in situ methods. An understanding of the interactions of the intramolecular forces in a cotton nanocomposite architecture is critical in the selection of methods which appropriates its functionality.
The
The cellulose structure of cotton fiber constitutes complex chain conformations based on its chirality, length and morphology which varies consistently based on the high degree of polymerization of cellulose chains in its fiber which is about 15,000 [58]. One of the major challenges in the synthesis of cotton nanocomposites is to ensure uniform dispersion of nanoparticles without particle aggregation. Nanoparticles aggregate due to high surface area, high surface energy and strong inter-particle attractions [59] leading to lower Gibb’s free energy making it detrimental to material performance [60]. The spatial distribution and nanoparticle assembly in a nanocomposite is primarily dependent on the delicate balance of intermolecular forces between nanoparticles within the matrix of its [61]. For proper particle dispersion, thermodynamic miscibility must be achieved [62]. Dispersion of the nanoparticles is highly dependent on the hydrogen bonding capacity of the cotton cellulose network. Where self-assembly of nanoparticles is strategically manipulated within a polymer matrix, it would result in a novel functionality of the forthcoming nanocomposite and expand its horizons for application due to its emerging properties such as water resistance, modulation of light, electrical conductivity and antibacterial sustenance [63]. It has to be noted that the ultimate performance of the nanocomposite is dependent on the interaction of the introduced nanoparticles and the cotton matrix which modulates the self-assembly architecture of the nanocomposite. Cotton fibers possess a backbone structure that is largely comprised of hydroxy groups which impart a strong affinity to water molecules, inducing microbial growth and raising the risk of contamination. The incorporation of the nanoparticle assembly however, renders the composite surface to be hydrophobic. Hydrogen bonding is the primary determinant in the spatial arrangement and self-assembly mechanism of molecules in cotton nanocomposites caused by the OH groups present in the glycoside backbones of the cotton cellulose fibers [57]. Hydrophobic interactions are also prevalent in cotton nanocomposites but are also responsible for the aggregation of nanoparticles in the structure [64]. Another force that participates in the self-assembly of nanoparticles in biopolymers during the in-situ synthesis of cotton nanocomposites is the van der Waals force, a short-ranged force, relatively weaker than hydrogen bonding, created by a transient dipole moment produced by an attractive force when nanoparticles move into close proximity [65].
Vajja et al., (2017) developed cotton nanocomposite material through the
The
An ex situ method used to form cotton nanocomposites of added value was by surface coating the material with metallic or metal oxide nanoparticles. Daoud et al. (2004) reported the deposition of anatase TiO2NPs on cotton and observed that the coated cotton nanocomposite had enhanced UV protection, antibacterial potential and self-cleaning properties [75]. Uddin et al. (2021) showed ex situ deposition of TiO2NPs on cotton fabric using the sol–gel method which demonstrated similar properties [76]. A nanocomposite of AgNp loaded with SiO2 nanoparticles was prepared using the sol–gel technique in which AgNps were generated using the
The
Cotton nanofibers are natural fibers which mostly constitute holocellulose (cellulose and hemicellulose) and lignin and has several advantages such as lower density, availability, biodegradability and exceptional mechanical properties which make it an ideal candidate as a polymer nanocomposite. The valorisation of agro residues of cotton would result in novel materials that could be used as fillers or reinforcement materials to form nanocomposites of potent value. Unlike other plants such as jute, flax and kenaf which are made up of only 25% cellulose and wood-based trees which contain 40–50% cellulose, cotton fibers are made up of 90% cellulose [82]. The cellulose in the cotton fibers are among the highest in molecular weight among all plant fibers and the most crystalline and fibrillated [83]. Cotton fiber comprises cellulose with 1,4-d-glucopyranose structural units [84] which accumulate as microfibrils arranged in regular pattern with excellent mechanical properties such as the Young’s Modulus and low thermal expansion [85]. Nanofibers generated from cellulose isolated from cotton fibers can be categorized as nanowires with aspect ratio beyond 1000, nanorods with aspect ratios between 3 and 5, nanoribbons and nanotubes with aspect ratios >10 [17]. Dried cotton fibers comprise large amounts of cellulose and hemi-cellulose which increase in tensile strength and durability when the impurities are removed. These cellulose based fibers are usually added as reinforcement material to generate nanocomposites needed in construction, automotive and electronics industry, as membranes for ultrafiltration, ion exchange and fuel cells and as binders in pharmaceuticals and cosmetic fillers [86]. Cellulose nanofibrils gives greater tensile strength compared to natural fibers and it has exceptionally large surface to volume ratio compared to its bulk form [87].
The extraction of cotton nanocellulose can be carried out using mechanical methods such as high-pressure homogenization, ball grinding, ultrasonication or high-speed blending [88] or chemical methods using acid hydrolysis with strong acids such as sulfuric acid or hydrochloric acid, oxidation with TEMPO (2,2,6,6-tetramethylpyperidine-l-oxyl) [89] or a combination of both mechanical and chemical methods [90]. It is found that acid hydrolysis removes the amorphous regions in the cotton fiber and generates nanocellulose with high crystallinity and uniform size distribution [89]. Sulfuric acid generates a more stable colloidal suspension of cellulose nanocrystals [24] and is preferred to hydrochloric acid which causes mass aggregation of cellulose nanocrystals because of the minimal surface charge that causes a lack of electrostatic repulsion force between the crystal particles [91]. Also, the hazards of inorganic acids and their corrosive nature are detrimental to the environment [92]. Mechanical processes generate nanofibers at a high success rate but the strong mechanical shearing forces causes disruption of the fibers, depict excessive energy consumption and homogenizer obstruction after prolonged use [88]. To elude the shortcomings presented by both the mechanical and chemical processes of nanocellulose extraction, pre-treatment with cellulase or enzymatic hydrolysis has been considered. Enzymatic hydrolysis is an appropriate pretreatment method used to disrupt interfibrillar cohesive forces and facilitate the disintegration of cotton fibers, while decreasing the size and degree of polymerization of cellulose fibers [93]. This method has been found to be highly selective and carried out at conditions with lower energy requirements [14]. Additionally, it replaces harmful solvents with biodegradable enzymes such as cellulases, which does not release hazardous emissions to the environment [94]. Cellulose is comprised of highly ordered crystalline regions interspersed with disorganized amorphous regions. The amorphous regions of cellulose are more susceptible to enzymatic degradation compared to the crystalline area. Cellulase enzyme has the potential of selective hydrolyzation of the amorphous region while maintaining the crystalline region, making it a process of choice to isolate cellulose nanocrystals. Therefore, this route has become increasingly popular as a sustainable method to prepare cellulose nanocrystals because of its high selectivity, mild conditions, and weak changes in surface chemistry [93]. Moreover, it complies with the principles of green chemistry as it leaves no carbon footprint, generates no hazardous waste and poses less water and energy consumption [95].
The addition of nanocellulose extracted from cotton as a reinforcing agent to a polymer system such as plastic, rubber or concrete improves the mechanical, thermodynamic and adsorption properties of the composite without changing the original qualities of the parent material [94]. Cotton fibers with a diameter in the range of 10–30 nm and a high aspect ratio are observed to improve the mechanical properties in a polymer composite for non-food packaging applications [96]. These nanocomposites have been postulated to hold tremendous potential in biomedicine as scaffolds in tissue engineering and for encapsulation in drug delivery [97]. The advances in mammalian cell culture technology are astounding. Here, nanocomposite biopolymers perform as biomimetic substrates for cell adhesion and proliferation. The nanotopography of substrates constructed from biomolecules such as collagen which includes surface roughness and porosity, influences interface interaction with mammalian cells or tissue that could improve cell adhesion and multiplication [98]. The incorporation of nanomaterials into these polymer matrixes can yield composites with the necessary properties for cell and tissue culture. Cotton based cellulose nanofibers (CCN) have a tremendous potential to be engineered for polymer composite reinforcement [91] as it mimics the structure of collagen in directionality and surface functionalization which is paramount to the adhesion, spreading and proliferation of cells [99].
Translating cotton based nanocellulose into polymer nanocomposites can be carried out using electrospinning, cast drying, freeze drying, vacuum assisted filtration, wet spinning, layer by layer assembly, micropatterning, melt blending, intercalated polymerization, sol-gel and solvent evaporation technique [100]. The solvent evaporation technique is the simplest method for nanocomposite synthesis which involves nanocellulose dispersion in polymer solution through energetic agitation followed by controlled evaporation of the solvent and composite film casting [101]. Li et al., (2014) prepared a nancomposite of cotton nanofiber in high density polyethylene (HDPE) using 2 different pretreatment methods. The first was blending the HDPE in a cotton CNF suspension, dehydrating and freeze drying the mixture followed by compounding and extrusion. This was a rapid, eco-friendly method as there were no chemical solvents involved in the process. In the second method, polyoxyethylene (PEO) was used as a dispersion agent to coat the cotton CNF before adding to HDPE granules and extraction. FESEM results revealed that both methods produced well dispersed CNF in HDPE and generated an excellent network structure of the cotton CNF/HDPE composites but the nanocomposite produced using the blending method was preferred as it demonstrated greater bending strength (MOR) and bending modulus (MOE) [102].
Nanocomposites have several advantages over conventional composites in their superior tensile strength, thermal capacity and barrier properties, biodegradability, recyclability and low weight [103]. Insertion of nanocellulose to biodegradable polymers to form bio-nanocomposites may improve the brittleness, poor barrier properties and low thermal stability of pure biodegradable polymers [104]. Much work has been carried out in recent times to explore the design of bionanocomposites en route to the development of higher quality bioplastics [105, 106].
A problem faced in generating cotton based cellulose nanocomposites is the limited dispersion of nanocellulose in polymers. This can be overcome by attaching a hydrophobic group to the surface of the cellulose matrix through esterification, acetylation or silanization which increases compatibility with the matrix. Solution casting is commonly used in the preparation of nanocomposite films but it its unsuitable for commercial scale production. Another method known as extrusion using melt processing has shown much promise for large scale production of cotton based cellulose nanocomposites [107]. However, for transforming research to industry and commercialization of cotton based cellulose nanocomposites, it is necessary to weigh the production costs, waste emissions, energy consumption, feasibility of the process and compliance to environmental ethics. Overall, the application prospects for nanocellulose appear to be very optimistic, but further research is needed to develop viable methods from laboratory to industrialization.
Nanocomposites are defined as multi-element materials with at least one element having a dimension of less than 100 nm [108]. In this chapter we have reviewed cotton based cellulose nanocomposites which are constructed by adding multifunctional nanoparticles to the cotton fabric using in situ or ex situ processes or by extracting nanocellulose structures from cotton fibers and incorporating it into polymer matrices. This results in novel nanocomposites with enhanced antimicrobial activity, polymer reinforcement and enhanced adhesion and adsorption in inert matrixes.
The introduction of metallic nanoparticles into cotton textiles has resulted in high performance multifunctional cotton nanocomposites which demonstrate excellent antimicrobial activity, water repellency, UV protection and antistatic finishes. These nanocomposites are gaining much interest particularly in generating antimicrobial material for protection against emerging pathogens. It is projected that further research in nanocomposite technology would decipher the details of the functional properties and performance of existing and emerging cotton nanocomposites and to determine the toxicity and safety of the generated fabrics. Additionally, there is a pressing need that the discoveries in the laboratory should be translated to commercial applications through the design of fabrication processes that favor cost effective, large scale production.
The incorporation of cotton nanocellulose into polymers as fillers to form reinforced nanocomposites also shows much promise particularly in the creation of chemical and biodegradable polymers of increased strength and tensile modulus and as scaffolds and support substrates in biomedicine. Yet there are issues that need to be addressed prior to translation into commercial viability such as the influence of the size and morphology of cotton nanocellulose fillers in the polymer matrix and the structural compatibility of the resulting polymer, biocompatibility of the nanocomposites in biomedical applications and the poor dispersion of cotton nanocellulose in the polymeric domain structure [109]. It is believed that these issues will be addressed aggressively in the near future to pave the way for the birth of a new breed of nanocomposite material using cotton nanostructures.
The author declares no conflict of interest.
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Wrzal"}]},{id:"43632",doi:"10.5772/53110",title:"The Importance of Cancer Cell Lines as in vitro Models in Cancer Methylome Analysis and Anticancer Drugs Testing",slug:"the-importance-of-cancer-cell-lines-as-in-vitro-models-in-cancer-methylome-analysis-and-anticancer-d",totalDownloads:5060,totalCrossrefCites:21,totalDimensionsCites:50,abstract:null,book:{id:"3002",slug:"oncogenomics-and-cancer-proteomics-novel-approaches-in-biomarkers-discovery-and-therapeutic-targets-in-cancer",title:"Oncogenomics and Cancer Proteomics",fullTitle:"Oncogenomics and Cancer Proteomics - Novel Approaches in Biomarkers Discovery and Therapeutic Targets in Cancer"},signatures:"Daniela Ferreira, Filomena Adega and Raquel Chaves",authors:[{id:"155129",title:"Prof.",name:"Raquel",middleName:null,surname:"Chaves",slug:"raquel-chaves",fullName:"Raquel Chaves"},{id:"157394",title:"M.Sc.",name:"Daniela",middleName:"Perneta",surname:"Ferreira",slug:"daniela-ferreira",fullName:"Daniela Ferreira"},{id:"157395",title:"Dr.",name:"Filomena",middleName:null,surname:"Adega",slug:"filomena-adega",fullName:"Filomena Adega"}]},{id:"24601",doi:"10.5772/22656",title:"Combination Chemotherapy in Cancer: Principles, Evaluation and Drug Delivery Strategies",slug:"combination-chemotherapy-in-cancer-principles-evaluation-and-drug-delivery-strategies",totalDownloads:4908,totalCrossrefCites:3,totalDimensionsCites:38,abstract:null,book:{id:"374",slug:"current-cancer-treatment-novel-beyond-conventional-approaches",title:"Current Cancer Treatment",fullTitle:"Current Cancer Treatment - Novel Beyond Conventional Approaches"},signatures:"Ana Catarina Pinto, João Nuno Moreira and Sérgio Simões",authors:[{id:"48598",title:"Prof.",name:"Sergio",middleName:null,surname:"Simoes",slug:"sergio-simoes",fullName:"Sergio Simoes"},{id:"54753",title:"Dr.",name:"Ana",middleName:null,surname:"Pinto",slug:"ana-pinto",fullName:"Ana Pinto"},{id:"54754",title:"Prof.",name:"Joăo",middleName:null,surname:"Moreira",slug:"joao-moreira",fullName:"Joăo Moreira"}]},{id:"22475",doi:"10.5772/24666",title:"Extracellular Matrix Microenvironment in Glioma Progression",slug:"extracellular-matrix-microenvironment-in-glioma-progression",totalDownloads:2505,totalCrossrefCites:14,totalDimensionsCites:27,abstract:null,book:{id:"355",slug:"glioma-exploring-its-biology-and-practical-relevance",title:"Glioma",fullTitle:"Glioma - Exploring Its Biology and Practical Relevance"},signatures:"Marzenna Wiranowska and Mumtaz V. Rojiani",authors:[{id:"58793",title:"Dr.",name:"Marzenna",middleName:null,surname:"Wiranowska",slug:"marzenna-wiranowska",fullName:"Marzenna Wiranowska"},{id:"137692",title:"PhD.",name:"Mumtaz",middleName:null,surname:"Rojiani",slug:"mumtaz-rojiani",fullName:"Mumtaz Rojiani"}]},{id:"23038",doi:"10.5772/27785",title:"Topical Administration of Anticancer Drugs for Skin Cancer Treatment",slug:"topical-administration-of-anticancer-drugs-for-skin-cancer-treatment",totalDownloads:4866,totalCrossrefCites:6,totalDimensionsCites:25,abstract:null,book:{id:"992",slug:"skin-cancers-risk-factors-prevention-and-therapy",title:"Skin Cancers",fullTitle:"Skin Cancers - Risk Factors, Prevention and Therapy"},signatures:"Stephânia Fleury Taveira and Renata Fonseca Vianna Lopez",authors:[{id:"71528",title:"Prof.",name:"Renata",middleName:null,surname:"Lopez",slug:"renata-lopez",fullName:"Renata Lopez"},{id:"72379",title:"Dr.",name:"Stephânia",middleName:null,surname:"Taveira",slug:"stephania-taveira",fullName:"Stephânia Taveira"}]}],mostDownloadedChaptersLast30Days:[{id:"54281",title:"Towards Metabolic Engineering of Podophyllotoxin Production",slug:"towards-metabolic-engineering-of-podophyllotoxin-production",totalDownloads:1710,totalCrossrefCites:3,totalDimensionsCites:3,abstract:"The pharmaceutically important anticancer drugs etoposide and teniposide are derived from podophyllotoxin, a natural product isolated from roots of Podophyllum hexandrum growing in the wild. The overexploitation of this endangered plant has led to the search for alternative sources. Metabolic engineering aimed at constructing the pathway in another host cell is very appealing, but for that approach, an in-depth knowledge of the pathway toward podophyllotoxin is necessary. In this chapter, we give an overview of the lignan pathway leading to podophyllotoxin. Subsequently, we will discuss the engineering possibilities to produce podophyllotoxin in a heterologous host. This will require detailed knowledge on the cellular localization of the enzymes of the lignan biosynthesis pathway. Due to the high number of enzymes involved and the scarce information on compartmentalization, the heterologous production of podophyllotoxin still remains a tremendous challenge. At the moment, research is focusing on the last step(s) in the conversion of deoxypodophyllotoxin to (epi)podophyllotoxin and 4′-demethyldesoxypodophyllotoxin by plant cytochromes.",book:{id:"5767",slug:"natural-products-and-cancer-drug-discovery",title:"Natural Products and Cancer Drug Discovery",fullTitle:"Natural Products and Cancer Drug Discovery"},signatures:"Christel L. C. Seegers, Rita Setroikromo and Wim J. Quax",authors:[{id:"196901",title:"Prof.",name:"Wim",middleName:null,surname:"Quax",slug:"wim-quax",fullName:"Wim Quax"},{id:"197867",title:"MSc.",name:"Christel L.C.",middleName:null,surname:"Seegers",slug:"christel-l.c.-seegers",fullName:"Christel L.C. Seegers"},{id:"197868",title:"Ms.",name:"Rita",middleName:null,surname:"Setroikromo",slug:"rita-setroikromo",fullName:"Rita Setroikromo"}]},{id:"24598",title:"Electrotherapy on Cancer: Experiment and Mathematical Modeling",slug:"electrotherapy-on-cancer-experiment-and-mathematical-modeling",totalDownloads:3917,totalCrossrefCites:1,totalDimensionsCites:4,abstract:null,book:{id:"374",slug:"current-cancer-treatment-novel-beyond-conventional-approaches",title:"Current Cancer Treatment",fullTitle:"Current Cancer Treatment - Novel Beyond Conventional Approaches"},signatures:"Ana Elisa Bergues Pupo, Rolando Placeres Jiménez and Luis Enrique Bergues Cabrales",authors:[{id:"64471",title:"Dr.",name:"Luis Enrique",middleName:null,surname:"Bergues Cabrales",slug:"luis-enrique-bergues-cabrales",fullName:"Luis Enrique Bergues Cabrales"}]},{id:"48215",title:"Local Metastasis in Head and Neck Cancer - an Overview",slug:"local-metastasis-in-head-and-neck-cancer-an-overview",totalDownloads:3047,totalCrossrefCites:0,totalDimensionsCites:3,abstract:null,book:{id:"4533",slug:"contemporary-issues-in-head-and-neck-cancer-management",title:"Contemporary Issues in Head and Neck Cancer Management",fullTitle:"Contemporary Issues in Head and Neck Cancer Management"},signatures:"Suwarna Dangore–Khasbage",authors:[{id:"82999",title:"Dr.",name:"Suwarna",middleName:null,surname:"Dangore-Khasbage",slug:"suwarna-dangore-khasbage",fullName:"Suwarna Dangore-Khasbage"}]},{id:"55831",title:"African Plants with Antiproliferative Properties",slug:"african-plants-with-antiproliferative-properties",totalDownloads:2103,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"Plant-derived compounds have been an integral component in man’s quest to discover ideal anticancer agents. A number of new agents are currently in clinical development with promising selective activity against cancer cell lines and cancer-related molecular targets. This book chapter discusses 14 of such compounds isolated from African plants from 15 plant families. Also contained in this book chapter are compounds from African plants that hold prospect as potential anticancer agents as informed by their in vitro and in vivo preclinical studies. It is, therefore, worthwhile that researchers in the African continent and the world over should keep on working on identifying biomolecules with potential in cancer management.",book:{id:"5767",slug:"natural-products-and-cancer-drug-discovery",title:"Natural Products and Cancer Drug Discovery",fullTitle:"Natural Products and Cancer Drug Discovery"},signatures:"Newman Osafo, Yaw Duah Boakye, Christian Agyare, Samuel\nObeng, Judith Edem Foli and Prince Amankwaah Baffour Minkah",authors:[{id:"182058",title:"Dr.",name:"Christian",middleName:null,surname:"Agyare",slug:"christian-agyare",fullName:"Christian Agyare"},{id:"186987",title:"Dr.",name:"Yaw Duah",middleName:null,surname:"Boakye",slug:"yaw-duah-boakye",fullName:"Yaw Duah Boakye"},{id:"196452",title:"Dr.",name:"Newman",middleName:null,surname:"Osafo",slug:"newman-osafo",fullName:"Newman Osafo"},{id:"201381",title:"Ms.",name:"Judith",middleName:null,surname:"Edem Foli",slug:"judith-edem-foli",fullName:"Judith Edem Foli"},{id:"201382",title:"Mr.",name:"Prince",middleName:"Amankwah Baffour",surname:"Minkah",slug:"prince-minkah",fullName:"Prince Minkah"},{id:"204731",title:"Mr.",name:"Samuel",middleName:null,surname:"Obeng",slug:"samuel-obeng",fullName:"Samuel Obeng"}]},{id:"53856",title:"Early-Stage Progression of Breast Cancer",slug:"early-stage-progression-of-breast-cancer",totalDownloads:1711,totalCrossrefCites:4,totalDimensionsCites:4,abstract:"Breast cancer can be defined as a group of diseases with heterogeneous origins, molecular profiles and behaviors characterized by uncontrolled proliferation of cells within the mammary tissue. Around one in eight women in the US will develop breast cancer in their lifetime, making it the second most frequently diagnosed cancer behind skin cancer [1]. In 2015, an estimated 231,840 cases of invasive carcinoma were diagnosed, and over 40,000 deaths were caused by breast cancer which accounts for almost 7% of all cancer mortality each year. In 2015, 60,290 cases of in situ breast cancer were diagnosed, representing over 14% of all new cancer cases among women and men. The steep increase in diagnosis of early‐stage breast cancer over the past 10 years is believed to be a result of more frequent mammography. However, since over half of these in situ lesions will not progress to invasive breast cancer, controversies have arisen about approaches to treatment and prevention of progression of early‐stage in situ breast cancer. Understanding the mechanisms of transition of normal breast to in situ pre‐neoplastic lesions and invasive breast cancer is currently a major focus of breast cancer research with implications for preventive and clinical management of breast cancer. In this review, we give an overview of current knowledge on the molecular and pathological changes that occur during early‐stage progression of breast cancer and describe some of the current models that are used to study this process.",book:{id:"5431",slug:"breast-cancer-from-biology-to-medicine",title:"Breast Cancer",fullTitle:"Breast Cancer - From Biology to Medicine"},signatures:"William Kietzman, Anna T. Riegel and Virginie Ory",authors:[{id:"190578",title:"Prof.",name:"Anna",middleName:null,surname:"Riegel",slug:"anna-riegel",fullName:"Anna Riegel"},{id:"190580",title:"Dr.",name:"Virginie",middleName:null,surname:"Ory",slug:"virginie-ory",fullName:"Virginie Ory"},{id:"190583",title:"MSc.",name:"William",middleName:null,surname:"Kietzman",slug:"william-kietzman",fullName:"William Kietzman"}]}],onlineFirstChaptersFilter:{topicId:"190",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"81836",title:"Perspective Chapter: Cervical Cancer Elimination by 2030—The W.H.O Goal: Neo Challenges and Next Gen Solutions “TIT for TAT”—The Community Competency Model of Raj ©",slug:"perspective-chapter-cervical-cancer-elimination-by-2030-the-w-h-o-goal-neo-challenges-and-next-gen-s",totalDownloads:12,totalDimensionsCites:0,doi:"10.5772/intechopen.104660",abstract:"Cervical Cancer is the fourth most common cancer among women, worldwide. It accounts for 600,000 new cases per year, and 340,000 deaths globally (WHO 2020 data). It causes a lot of maladies and suffering for women, in the age group of 30–60 years, especially in the poor community of developing countries. Cervical cancer is a great public health problem and is a cause of grave concern for the health system in Low-Middle-Income Countries—LMIC. But cervical cancer is amenable for early detection and successful treatment of precancer stages. Human Papilloma Virus—HPV vaccines offer a high level of primordial prevention, against cervical cancer. Therefore, the World Health Organization, in 2018, has called for “Elimination of Cervical Cancer by 2030.” The objective is to reduce the incidence rate of cervical cancer to below 4/100,000, by the year 2030. This leads to many “Neo Challenges” and also opens the door for “Next Gen Solutions”. The author, with vast experiences in his Cervical Cancer Screening Projects of IARC/ WHO, at Tamil Nadu, India, during 2000–2007, advocates a strategy called “TIT for TAT—The Community Competency model of Raj©.”",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Rajamanickam Rajkumar"},{id:"81872",title:"Benign Prostatic Hyperplasia: Epidemiology, Pathophysiology, and Clinical Manifestations",slug:"benign-prostatic-hyperplasia-epidemiology-pathophysiology-and-clinical-manifestations",totalDownloads:11,totalDimensionsCites:0,doi:"10.5772/intechopen.104823",abstract:"The prostate secretes 20% of the seminal fluid. One of its main pathologies is benign prostatic hyperplasia (BPH), the most common benign disease in older men. It has an 8–10% prevalence in men 40 years of age and older, increasing to more than 90% in men over 90 years, with lower urinary tract symptoms being one of its main complications. Although the etiology of BPH is not still fully known, testosterone and estradiol have shown a permissive role. Likewise, other factors have emerged, such as inflammation, growth factors, and prolactin, which influence the development of BPH. These factors act through binding to specific receptors, intervening in BPH and prostate cancer development. Existing treatments significantly reduce clinical symptoms, including lower urinary tract symptoms. However, it is a nonpreventable disease; some factors can reduce its incidence: diet, physical activity, and moderate consumption of alcohol and tobacco, some of which have been proposed to have a protective role. Therefore, this chapter aims to update the preclinical and clinical evidence on the etiology of this disease, briefly describing the epidemiology, clinical manifestations, and therapeutic and preventive modalities in managing BPH.",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Luz Irene Pascual Mathey"},{id:"81779",title:"Cancer Genes and Breast Cancers",slug:"cancer-genes-and-breast-cancers",totalDownloads:21,totalDimensionsCites:0,doi:"10.5772/intechopen.104801",abstract:"Cancer is the name given to all malignant tumors, the main reason for which is uncontrolled growth, and the tumor, which has become a mass as a result of uncontrolled cell proliferation, also attacks the surrounding cells and envelops the whole body (metastasis) in the later stages of the disease. Although cancer is an important health problem, it is not a common disease in childhood. On the other hand, statistics show that cancer affects one in three adults, causes up to 20% of all deaths, and covers about 10% of treatment costs in developed countries. Although it is known that cancer develops under the influence of genetic and environmental factors, environmental factors are more prominent in the formation of some types of cancer. Breast cancer is one of the cancer types known to have tumor suppressor genes in its etiology. These tumor suppressor genes are BRCA1 and BRCA2 genes. Studies have shown that these two genes are particularly effective in the development of familial breast cancers. These types of cancers occur much earlier than non-familial cancers. The research, two genes; It has shown that it is especially effective in the development of familial breast cancers.",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Metin Budak and Hatice Segmen"},{id:"81028",title:"Molecular Genetic Mechanisms in Cancers of Keratinocytic Origin",slug:"molecular-genetic-mechanisms-in-cancers-of-keratinocytic-origin",totalDownloads:21,totalDimensionsCites:0,doi:"10.5772/intechopen.103134",abstract:"Keratinocytic cancers (KC) comprise a group of diseases that have a broad spectrum clinically and pathologically. At one end of the spectrum are benign proliferations (acanthomas), and at the other end are malignant tumors with aggressive growth and metastatic potential. Traditionally, about 80% of KC cases have basal cell carcinoma (BCC) and 20% have cutaneous squamous cell carcinoma (cSCC). Both tumors have different phenotypic features due to different oncogenic pathways. cSCC is biologically different and requires a different approach due to the higher risk of local recurrence, metastasis and death. Genetic factors play an important role in the development of KC. Family and family history studies, the presence of KC as a feature of rare hereditary syndromes, and genetic association studies give us clues in this regard. More than 20 genetic syndromes associated with KC have been described. Some syndromes are associated with multiple BCC, some with multiple cSCC, and some with both BCC and cSCC. Environmental risk factors include exposure to ultraviolet light radiation and immunosuppression in both tumors. Exposure to ionizing radiation is most common in BCC, while smoking and photosensitive drug use are among the environmental risk factors for cSCC. Molecular, epidemiological, and clinical studies will help better understand the cellular processes involved in tumorigenesis, and develop new strategies for treating and preventing KCs.",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Yildiz Gürsel Ürün"},{id:"80850",title:"Splicing in Cancer",slug:"splicing-in-cancer",totalDownloads:35,totalDimensionsCites:0,doi:"10.5772/intechopen.102707",abstract:"Defects in splicing, especially alternative splicing have been frequently found in cancers. Mutations in the splicing regulatory elements of important genes involved in cancers or the genes encoding regulatory splicing machinery could play a key role in carcinogenesis. Alterations in regulator factors in splicing have emerged as a new class of oncoproteins and tumor suppressor genes. Understanding the molecular mechanism of how defects in splicing and in particular alternative splicing are involved in carcinogenesis, could lead to new strategies to cancer therapy. Here, we review the molecular mechanism of splicing and regulatory factors involved in alternative splicing, as well as the aberrant splicing that affects cancer hallmarks. Finally, we summarize new approaches in cancer therapy based on splicing.",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Mehdi Moghanibashi and Parisa Mohamadynejad"},{id:"80759",title:"Molecular Epidemiology of High-Risk Human Papillomavirus Infection in Burkina Faso",slug:"molecular-epidemiology-of-high-risk-human-papillomavirus-infection-in-burkina-faso",totalDownloads:55,totalDimensionsCites:0,doi:"10.5772/intechopen.102327",abstract:"The aim of the present study was to determine the distribution of high-risk human papillomavirus (HR-HPV) genotypes in childbearing age women, teenage girls, HIV-infected women, women with high-grade precancerous lesions and cervical cancer, sex workers, men, and otolaryngology tumor cases in Burkina Faso. This descriptive cross-sectional study with several target groups, consisted of 2386 samples from Burkina Faso. HR-HPV genotypes were characterized using real-time multiplex PCR. The prevalence of HR-HPV ranged from 15.63 to 72.31% depending on the target population and the nature of the samples. The most predominant genotypes in descending order were HPV-56, HPV-52, HPV-39, HPV-59, HPV-51, HPV-35, HPV-31, HPV-18, HPV-68, HPV-16, HPV-66, HPV-58, HPV-45, and HPV-33. The results of the present study show a wide variation in the distribution of HR-HPV genotypes in Burkina Faso. Genotypes 16 and 18 covered by HPV vaccines only accounted for 32.23% of HR-HPV cases.",book:{id:"10793",title:"Molecular Mechanisms in Cancer",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg"},signatures:"Théodora Mahoukèdè Zohoncon, Rogomenoma Alice Ouedraogo, Florencia Wendkuuni Djigma, Lassina Traore, Teega-Wendé Clarisse Ouedraogo, Maimouna Ilboudo, Regine Ilboudo, Catherine Salambanga, Sindimalgdé Patricia Guigma, Sessi Frida Tovo, Mah Alima Esther Traore, Prosper Bado, Ali Kande, Cyrille Bisseye, Abdoul Karim Ouattara, Ina Marie Angèle Traore, Djeneba Ouermi, Tani Sagna, Albert Théophane Yonli, Wendyam Marie Christelle Nadembega, Dorcas Obiri-Yeboah, Yvette Marie Chantal Gyebre, Olga Mélanie Lompo, Charlemagne Marie Ragnag-Newende Ouedraogo and Jacques Simpore"}],onlineFirstChaptersTotal:12},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:330,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:9,numberOfPublishedChapters:139,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:122,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:21,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. 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Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. 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He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. 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He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. 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His present research includes organic synthesis, drug discovery and development, biochemistry, nanoscience, and nanotechnology.",institutionString:"Visiting Scientist at Lipid Nanostructures Laboratory, Centre for Smart Materials, School of Natural Sciences, University of Central Lancashire",institution:null},{id:"428125",title:"Dr.",name:"Vinayak",middleName:null,surname:"Adimule",slug:"vinayak-adimule",fullName:"Vinayak Adimule",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/428125/images/system/428125.jpg",biography:"Dr. Vinayak Adimule, MSc, Ph.D., is a professor and dean of R&D, Angadi Institute of Technology and Management, India. He has 15 years of research experience as a senior research scientist and associate research scientist in R&D organizations. He has published more than fifty research articles as well as several book chapters. He has two Indian patents and two international patents to his credit. Dr. Adimule has attended, chaired, and presented papers at national and international conferences. He is a guest editor for Topics in Catalysis and other journals. He is also an editorial board member, life member, and associate member for many international societies and research institutions. His research interests include nanoelectronics, material chemistry, artificial intelligence, sensors and actuators, bio-nanomaterials, and medicinal chemistry.",institutionString:"Angadi Institute of Technology and Management",institution:null},{id:"284317",title:"Prof.",name:"Kantharaju",middleName:null,surname:"Kamanna",slug:"kantharaju-kamanna",fullName:"Kantharaju Kamanna",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284317/images/21050_n.jpg",biography:"Prof. K. Kantharaju has received Bachelor of science (PCM), master of science (Organic Chemistry) and Doctor of Philosophy in Chemistry from Bangalore University. He worked as a Executive Research & Development @ Cadila Pharmaceuticals Ltd, Ahmedabad. He received DBT-postdoc fellow @ Molecular Biophysics Unit, Indian Institute of Science, Bangalore under the supervision of Prof. P. Balaram, later he moved to NIH-postdoc researcher at Drexel University College of Medicine, Philadelphia, USA, after his return from postdoc joined NITK-Surthakal as a Adhoc faculty at department of chemistry. Since from August 2013 working as a Associate Professor, and in 2016 promoted to Profeesor in the School of Basic Sciences: Department of Chemistry and having 20 years of teaching and research experiences.",institutionString:null,institution:{name:"Rani Channamma University, Belagavi",country:{name:"India"}}},{id:"158492",title:"Prof.",name:"Yusuf",middleName:null,surname:"Tutar",slug:"yusuf-tutar",fullName:"Yusuf Tutar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/158492/images/system/158492.jpeg",biography:"Prof. Dr. Yusuf Tutar conducts his research at the Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, University of Health Sciences, Turkey. He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"94311",title:"Prof.",name:"Martins",middleName:"Ochubiojo",surname:"Ochubiojo Emeje",slug:"martins-ochubiojo-emeje",fullName:"Martins Ochubiojo Emeje",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94311/images/system/94311.jpeg",biography:"Martins Emeje obtained a BPharm with distinction from Ahmadu Bello University, Nigeria, and an MPharm and Ph.D. from the University of Nigeria (UNN), where he received the best Ph.D. award and was enlisted as UNN’s “Face of Research.” He established the first nanomedicine center in Nigeria and was the pioneer head of the intellectual property and technology transfer as well as the technology innovation and support center. 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He received his post-doctoral training in oncology and cancer proteomics for two years at the Cancer Research Institute of Human Medical University in China. In 2001, he went to the University of Tennessee Health Science Center (UTHSC) in USA, where he was a post-doctoral researcher and focused on mass spectrometry and cancer proteomics. Then, he was appointed as an Assistant Professor of Neurology, UTHSC in 2005. He moved to the Cleveland Clinic in USA as a Project Scientist/Staff in 2006 where he focused on the studies of eye disease proteomics and biomarkers. He returned to UTHSC as an Assistant Professor of Neurology in the end of 2007, engaging in proteomics and biomarker studies of lung diseases and brain tumors, and initiating the studies of predictive, preventive, and personalized medicine (PPPM) in cancer. In 2010, he was promoted to Associate Professor of Neurology, UTHSC. Currently, he is a Professor at Xiangya Hospital of Central South University in China, Fellow of Royal Society of Medicine (FRSM), the European EPMA National Representative in China, Regular Member of American Association for the Advancement of Science (AAAS), European Cooperation of Science and Technology (e-COST) grant evaluator, Associate Editors of BMC Genomics, BMC Medical Genomics, EPMA Journal, and Frontiers in Endocrinology, Executive Editor-in-Chief of Med One. He has\npublished 116 peer-reviewed research articles, 16 book chapters, 2 books, and 2 US patents. 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He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. 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He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"418340",title:"Dr.",name:"Jyotirmoi",middleName:null,surname:"Aich",slug:"jyotirmoi-aich",fullName:"Jyotirmoi Aich",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038Ugi5QAC/Profile_Picture_2022-04-15T07:48:28.png",biography:"Biotechnologist with 15 years of research including 6 years of teaching experience. Demonstrated record of scientific achievements through consistent publication record (H index = 13, with 874 citations) in high impact journals such as Nature Communications, Oncotarget, Annals of Oncology, PNAS, and AJRCCM, etc. Strong research professional with a post-doctorate from ACTREC where I gained experimental oncology experience in clinical settings and a doctorate from IGIB where I gained expertise in asthma pathophysiology. A well-trained biotechnologist with diverse experience on the bench across different research themes ranging from asthma to cancer and other infectious diseases. An individual with a strong commitment and innovative mindset. Have the ability to work on diverse projects such as regenerative and molecular medicine with an overall mindset of improving healthcare.",institutionString:"DY Patil Deemed to Be University",institution:null},{id:"349288",title:"Prof.",name:"Soumya",middleName:null,surname:"Basu",slug:"soumya-basu",fullName:"Soumya Basu",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035QxIDQA0/Profile_Picture_2022-04-15T07:47:01.jpg",biography:"Soumya Basu, Ph.D., is currently working as an Associate Professor at Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. With 16+ years of trans-disciplinary research experience in Drug Design, development, and pre-clinical validation; 20+ research article publications in journals of repute, 9+ years of teaching experience, trained with cross-disciplinary education, Dr. Basu is a life-long learner and always thrives for new challenges.\r\nHer research area is the design and synthesis of small molecule partial agonists of PPAR-γ in lung cancer. She is also using artificial intelligence and deep learning methods to understand the exosomal miRNA’s role in cancer metastasis. Dr. Basu is the recipient of many awards including the Early Career Research Award from the Department of Science and Technology, Govt. of India. She is a reviewer of many journals like Molecular Biology Reports, Frontiers in Oncology, RSC Advances, PLOS ONE, Journal of Biomolecular Structure & Dynamics, Journal of Molecular Graphics and Modelling, etc. She has edited and authored/co-authored 21 journal papers, 3 book chapters, and 15 abstracts. She is a Board of Studies member at her university. She is a life member of 'The Cytometry Society”-in India and 'All India Cell Biology Society”- in India.",institutionString:"Dr. D.Y. Patil Vidyapeeth, Pune",institution:{name:"Dr. D.Y. Patil Vidyapeeth, Pune",country:{name:"India"}}},{id:"354817",title:"Dr.",name:"Anubhab",middleName:null,surname:"Mukherjee",slug:"anubhab-mukherjee",fullName:"Anubhab Mukherjee",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y0000365PbRQAU/ProfilePicture%202022-04-15%2005%3A11%3A18.480",biography:"A former member of Laboratory of Nanomedicine, Brigham and Women’s Hospital, Harvard University, Boston, USA, Dr. Anubhab Mukherjee is an ardent votary of science who strives to make an impact in the lives of those afflicted with cancer and other chronic/acute ailments. He completed his Ph.D. from CSIR-Indian Institute of Chemical Technology, Hyderabad, India, having been skilled with RNAi, liposomal drug delivery, preclinical cell and animal studies. He pursued post-doctoral research at College of Pharmacy, Health Science Center, Texas A & M University and was involved in another postdoctoral research at Department of Translational Neurosciences and Neurotherapeutics, John Wayne Cancer Institute, Santa Monica, California. In 2015, he worked in Harvard-MIT Health Sciences & Technology as a visiting scientist. He has substantial experience in nanotechnology-based formulation development and successfully served various Indian organizations to develop pharmaceuticals and nutraceutical products. He is an inventor in many US patents and an author in many peer-reviewed articles, book chapters and books published in various media of international repute. Dr. Mukherjee is currently serving as Principal Scientist, R&D at Esperer Onco Nutrition (EON) Pvt. Ltd. and heads the Hyderabad R&D center of the organization.",institutionString:"Esperer Onco Nutrition Pvt Ltd.",institution:null},{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/319365/images/system/319365.png",biography:"Manash K. Paul is a Principal Investigator and Scientist at the University of California Los Angeles. He has contributed significantly to the fields of stem cell biology, regenerative medicine, and lung cancer. His research focuses on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering lung stem cell niche, pulmonary disease modeling, immuno-oncology, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration and detecting the metastatic phenotype of lung cancer via machine-learning-based analyses of exosomal signatures. Dr. Paul has published in more than fifty peer-reviewed international journals and is highly cited. He is the recipient of many awards, including the UCLA Vice Chancellor’s award, a senior member of the Institute of Electrical and Electronics Engineers (IEEE), and an editorial board member for several international journals.",institutionString:"University of California Los Angeles",institution:{name:"University of California Los Angeles",country:{name:"United States of America"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. He is currently working on the protective activity of phenolic compounds in disorders associated with oxidative stress and inflammation.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Dr.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329248",title:"Dr.",name:"Md. Faheem",middleName:null,surname:"Haider",slug:"md.-faheem-haider",fullName:"Md. Faheem Haider",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329248/images/system/329248.jpg",biography:"Dr. Md. Faheem Haider completed his BPharm in 2012 at Integral University, Lucknow, India. In 2014, he completed his MPharm with specialization in Pharmaceutics at Babasaheb Bhimrao Ambedkar University, Lucknow, India. He received his Ph.D. degree from Jamia Hamdard University, New Delhi, India, in 2018. He was selected for the GPAT six times and his best All India Rank was 34. Currently, he is an assistant professor at Integral University. Previously he was an assistant professor at IIMT University, Meerut, India. He has experience teaching DPharm, Pharm.D, BPharm, and MPharm students. He has more than five publications in reputed journals to his credit. Dr. Faheem’s research area is the development and characterization of nanoformulation for the delivery of drugs to various organs.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/system/329795.png",biography:"Dr. Mohd Aftab Siddiqui is an assistant professor in the Faculty of Pharmacy, Integral University, Lucknow, India, where he obtained a Ph.D. in Pharmacology in 2020. He also obtained a BPharm and MPharm from the same university in 2013 and 2015, respectively. His area of research is the pharmacological screening of herbal drugs/natural products in liver cancer and cardiac diseases. He is a member of many professional bodies and has guided many MPharm and PharmD research projects. Dr. Siddiqui has many national and international publications and one German patent to his credit.",institutionString:"Integral University",institution:null}]}},subseries:{item:{id:"88",type:"subseries",title:"Marketing",keywords:"Consumer Trends, Consumer Needs, Media, Pricing, Distribution, Branding, Innovation, Neuromarketing",scope:"