Results of EDS analysis of nanotube layers obtained by anodizing at 20V for 2h in 1M H3PO4 containing 0.2; 0.3; 0.4% wt. HF .
Properties of the oxide layers on titanium and its implant alloys can be tailored to desired applications by anodizing parameters. Electrochemical oxidation in various electrolytes and different polarization regimes may shape the morphology, structure and chemical composition of oxide layers to enhance the use of titanium materials in electronics, photovoltaic and medicine. Phosphate electrolytes play specific role in the anodizing process. Besides forming compact barrier layer they enable also to form porous and nanostructural oxide layers enriched with phosphates, which enhance their bioactivity.
The formation of anodic layers: thick or thin, compact or porous, gel-like and nanostructural on titanium and its alloys Ti6Al4V and Ti6Al7Nb in phosphoric acid solutions of different concentrations is described in this charter. Basing on morphological and chemical composition analysis (SEM, XPS) as well as on the electrochemical examination the influence of electrolyte composition on enrichment of surface oxide layers with phosphates and fluorides, enhancing their bioactivity, is presented. Studies to use Ti/titania systems as the platforms of the electrochemical biosensors to detect H2O2 and glucose proved the opportunity to use the nanotubular titania material as a platform for the 2rd generation biosensors.
2. Oxide layers on titanium and its implant alloys formed in H3PO4 solutions
Anodic films formed on titanium and its alloys are of great interest due to the industrial applications of metal covered with oxide layers of various and unique properties [1 -7]. These layers have been investigated extensively by many authors [8 -11]. Thick oxide layers on titanium, obtained by anodizing, provide improved resistance to local corrosion . Anodizing can result in the adsorption and incorporation of inorganic and organic, biologically important species, e.g. phosphate ions, into the oxide layer. Such surface layers, desirable for medical implants, are not only corrosion resistant in a biological environment, but also compatible with tissue response [13 -15]. Anodizing titanium and its alloys has been investigated in a wide range of parameters [16 -20], which include also the participation of the electrolyte components, e.g. anions, in the formation of anodic films [21, 22].
Studies in this field [24, 25] have shown that, phosphate ions can be incorporated into the anodic layer on titanium and Ti-6Al-4V, and in turn stimulate the formation of the bio-compatible hydroxyapatite .
Anodizing in phosphate solutions exhibit some advantages over other acid and base electrolytes. First of all less corrosive attack of phosphoric acid on titanium and its alloys, when compared with other acidic media, is related to the strong adsorption of phosphate anions on the surface [27,28]. Although unalloyed titanium is resistant  to naturally aerated pure solutions of phosphoric acid up to 30 % wt. concentration (~3.6 M) in phosphoric acid of lower concentrations (0.5-4 M), mainly non-dissociated acid molecules and H2PO4- of phosphate ions exist [29,30] and they exhibit a strong affinity or complexing power towards most metal cations.
Thus, anodizing in phosphate solutions leads to the incorporation of phosphate ions into the oxide layers on titanium and Ti-6Al-4V [5 -8] influencing their bioactivity and stimulating deposition of the biocompatible hydroxyapatite. The latter may be used to shape properties of titanium implant materials for medical purposes.
2.1. Thin porous anodic layers
Anodic layers on pure Ti and its alloys Ti6Al4V ELI and Ti6Al7Nb (ASTM F136-84), alloys in the annealed condition) can be formed by anodizing carried out at ambient pressure and room temperature in non-deaerated electrolyte solutions of 0.5 M H3PO4. Both techniques, the galvanostatic at anodizing current density values varied in the range of 0.1-0.5 Am-2 and the potentiostatic at up to 60V  are used. The oxide layers, 30-120nm thick, enriched with phosphorus are formed in these conditions. With mechanical and chemical pre-treatment applied to titanium and its alloys: Ti6Al4V ELI and Ti6Al7Nb (Timet Ltd, UK) [12 -20], the layers obtained at 60V in phosphoric acid are golden and porous [11 , 24 -26] (Fig. 2) and they show very stable values of currents in passive region up to 1 V (SCE) (Fig.3) . At other polarization parameters however layers of different thickness and colorization are formed . Due to the presence of phosphates in the anodic layers they are highly bioactive in comparison to oxides formed in other electrolytes. Just after 9 days in the SBF solution (Simulated Body Fluid) they are covered with hydroxyapatite deposists (Fig. 2) .
The bilayer structure of compact oxide covered with HAp particles can be demonstrated also in the impedance tests (Fig. 3). The first time constant in Bode diagrams in range of the high and intermediate frequencies confirmed the high
To investigate the effect of phosphoric acid concentration (0.5 - 4 M) on the anodising of titanium and its alloys the galvanostatic and potentiodynamic techniques have been applied [11,19]. Particularly, the galvanostatic method with low current densities, up to 0.6 Am-2, applied in order to minimize side effects (ie. oxygen evolution), and more importantly to determine processes responsible for the growth of the oxide layer on the anodised metal at the early stages of its formation, allowed to observe the abnormal behaviour of titanium at anodizing. In Fig. 4 the results presenting the minimum rates of potential growth at initial stages of galvanostatic anodising in 2 M H3PO4 solutions are shown.
Also the anodic polarization curves for titanium in electrolytes of different concentration show various shapes and different slopes in active-passive region (Fig.5).Potentiodynamic control at a comparable rate in 2 M H3PO4 applied to titanium and two of its implant alloys, Ti6Al4V ELI and Ti6Al7Nb, revealed a shift in corrosion potential toward the anodic direction with the lowest current densities in the passive region. This was possibly due to the effect of adsorption of phosphate ions onto the surface layer.
2.2. Gel like layers on anodic titania
Active-passive transition of titanium in phosphoric acid solutions of 0.5-4 M [11,19] reveals that the growth of anodic layer is affected proportionally by the applied anodic potential, but shows the unusual influence of electrolyte concentration. Under galvanostatic conditions at low current densities (0.1-0.6 Am-2) the slope of dE/dt shows the minimum at the concentration ~2 M H3PO4 (Fig. 4), which is resulted due to a coating of an oxide film by an additional gel-like layer during anodizing, [28, 29], similar to the one observed in other media on aluminum.
It was found that the active–passive transition was a process in which an inhibiting effect of phosphate ions on a dissolution of oxide layer was observed during anodizing . Typical examples of voltage
SEM/EDS examination confirm the evidence of two-layered surface film. Such layers presented in Fig. 6, show the whole surface covered by a gel-like layer of H3PO4×0.5H2O.
The SEM/EDS examinations reveal that thin films of anodic titania oxide are covered by gel-like layer with crystalline phosphates nuclei inside. Phosphates deposits are few in layers formed in 0.5 M H3PO4, but numerous and uniformly dispersed in a surface oxide of sample anodised in 2 M H3PO4. However, the oxide and phosphates are covered with the additional layer consisting of 76.3±3.6 wt.% of phosphorus and 23.7±1.5wt.% of oxygen (Fig.6a and 6b).
The EIS spectra of titanium after anodizing at 0.5 A/m2 in 0.5-2 M H3PO4 (Fig. 7), exhibit a behavior typical of a metallic material covered by a porous film which is exposed to an electrolytic environment . Two time constants are seen in the spectra: the first in the high-frequency part arises from the ohmic electrolyte resistance and the impedance resulting from the penetration of the electrolyte through a porous film, and the second in low-frequency part accounts for the processes at the substrate/electrolyte interface.
The EIS data can be fitted to the equivalent circuit in Fig. 7c, which consists of a solution resistance Rs, the capacitance Cp of the barrier layer, the charge transfer resistance associated with the penetration of the electrolyte through the pores Rp; and the polarization resistance of the barrier Rb as well as the electrical double-layer capacitance at the substrate/electrolyte interface Cb. In the case of surface layer formed in 2 M H3PO4 the specimen is covered by a passive oxide film of higher impedance (Fig.7a). The significant increase of the resistance Rb and Rp values for the 2 M H3PO4 anodized samples, over those determined for the 0.5 M H3PO4 anodized titanium, confirm that the EIS results are complementary to those obtained by
Titanium as metal very sensitive to the pre-treatment , due to the polishing, rinsing with water and drying, is usually covered by an air-formed oxide film and on immersion into acid solution shows potentials of the active-transition region [32-34]. However, in solutions of low pH may become active. In sulphate solution the anodic oxide film on titanium dissolves giving Ti3+ ions . Typical activation behaviour and slow decrease in the open-circuit potential (–0.3 V SCE), is observed on immersing titanium into 1M HCl [35,36]. Titanium behaves differently in H3PO4 solutions. Although the values of
In acidic solutions within the cathodic region, the only oxide dissolution is reaction (1). This reaction determines the potential-current changes in active-passive region of titanium in 0.5 M and 3-4 M H3PO4, whereas due to the shift of the corrosion potential
This attribution agrees with Morligde’s et. al. results on aluminum . Both reactions: the phosphates adsorption and gel-like layer formation are non-faradaic, but are competitive towards the oxide dissolution (reaction 1) with regard to proton consumption. The advantageous effect of these two reactions on the anodizing, may be attributed to an inadequate supply of H+ ions to keeping up with the demand for the reaction (1) of oxide dissolution. The increasing coverage of the anodized titanium surface by phosphates ions with the electrolyte concentration provides the evidence of a direct influence of electrolyte anions in suppressing the formation of dissolved titanium ions. According to potential/pH diagram for P-Ti-H2O system , H3PO4×0.5H2O, the product of reaction 5, is stable thermodynamically in solutions of pH ranging to 3.
Thus, due to the applied anodizing conditions formation of either thin and porous oxide layer [11-23] or gel-like phosphates rich layer of H3PO4×0.5H2O [11 , 24 -29, 42], covering thicker oxide layer on titanium can be obtained.
2.3. Bioactive layer
Apart from mechanical properties and biocompatibility, which make titanium and its alloys the materials of choice for various applications (artificial hip and knee joints, dental prosthetics, vascular stents, heart valves) also enhancement of bone formation is desired feature of a metallic implant developed through adequate surface treatments to obtain proper osseointegration.
Fast deposition of hydroxyapatite (HAp) coatings on titanium and its alloys Ti6Al4V and Ti6Al7Nb substrates anodised in H3PO4 was observed [21,23,25]. Anodizing in 0.5 M H3PO4, which produces phosphates enriched porous sub-surface layer on of titanium and its alloys Ti6Al4V and Ti6Al7N  or anodizing in 2 M H3PO4 which generates phosphates rich gel-like layer [31,42] may be used to enhance hydroxyapatite (HAp) deposition (Fig.8). For the latter anodic layers soaking the anodised substrates in simulated body fluid (SBF) resulted in the deposition of a uniform coating in 24 hours (Fig. 9). SEM and EDS investigations revealed that after 9 days thick coating consists of HAp globular of diameter varied from 100 to 300 nm aggregates. The Ca-O-P deposits merge in large clusters and they are seen in large numbers on both alloys, particularly on Ti6Al4V anodized in 2 M H3PO4.
SEM observations (Fig.8) and EDS microanalysis indicate the presence of deposits dispersed on the surface of anodised titanium and its implant alloys. However, deposits are are non-uniformly dispersed on a surface. Titanium and its two alloys anodised in 0.5M H3PO4 are covered with very thin oxide layer, which includes numerous and more scattered Ca-O-P deposits of diameter varied from 200 to 800 nm, suggesting the heterogeneous nucleation of Ca-O-P on TiO2 covered surface. Althogh just after 24 hours deposits are seen on the surface of the 3 materials (more deposists on the Ti6Al4V alloy) the continuous films cover the whole surfaces after 9 days in SBF solution. At higher magnification it is seen that the film on titanium is formed of more flatter layer of deposits and broken layer of titanium oxides with titanium phosphates, whereas film on both alloys comprise small globules of Ca-O-P. The ratio of Ca/P ranging from 1.26 to 1.42 corresponds to non-steochiometric hydroxyapatite.
Impedance (EIS) spectra (Fig. 9) recorded during immersion of the anodized titanium and the Ti6Al4V alloy in simulated body fluid (SBF) for titanium and Ti6Al4V alloy show changes in capacitance and structure of surface layers as well as differences between coatings on titanium and its alloy and confirm the SEM observations (Fig. 8). Titanium exhibits two-layered structure: the inner oxide layer is covered by an outer layer of more or less uniformly distributed various size Ca-P-O deposits. On contrary the Ti6Al4V alloy is coated by a more uniform and dense layer of deposits (Fig. 8 c,d) and much lower concentration of titanium oxide on a surface .
3. Nanostructural oxide layer formed in phosphate solutions
In the last 20 years anodizing has been also used as a method to form nanooxides on metal surfaces. Formation of self-organized titania nanotubes with high level organization of pores on large surfaces [43 -48] became very useful technology applied to many purposes, i.e. to modification of surgical implant surfaces and to biomedical sensing. Titania nanotubes, just like barrier type titania, combine very well with osseous tissue and can be a perfect basis for osteoblasts in surgical implants. Studies focused on controlling the size and arrangement of pores [49 -54], aiming at bone ingrowth and on use of titania nanotubes platform for biosensing, due to their capability to combine with e.g. enzymes, proteins or biological cells, brought promising results [55,56].
Formation of nanotubes at different polarization parameters in various electrolytes [57 -60] as well as various scan rates during the very first seconds of anodizing, may help tailoring the oxides for effective implantation and improve their properties for biomedical and sensor applications. For the latter applications titania nanotubes require better ordering ie. controlled diameter achieved during improved oxide growth kinetics. For the last 5 years in several papers [61 -64] it has been revealed that the value of polarisation determines the diameter of nanotubes. Every additional 5V of potential increases the nanotubes diameter of about 20nm, whereas the time of anodizing determines the length of the nanotube layer. Moreover, the low pH and organic aqueous solutions assure more regular shapes of nanotubes.
3.1. Nanotubes on titanium in phosphate solutions
Electrochemical oxidization of titanium can be carried out in electrolytes with or without HF additives [65 -67]. Attempts to assess the optimal scan rate/fluoride concentration ratio for formation of structurally uniform nanotubes  revealed that 1M H3PO4+0.3% wt. HF is the most proper electrolyte for anodizing at 0.5Vs-1. To study the effect of phosphates concentration, layers of titania nanotubes were produced in electrolytes of different phosphoric acid concentration. Their properties as the future coatings on titanium for medical uses were characterized by SEM/EDS observations and capacitance tests in simulated body fluids. Formation of oxide layers on titanium in phosphoric acid solutions with additions of fluoride ions  at 25ºC, is usually carried out in two stages: the first stage potentiodynamic to the desired potential and the second stage, potentiostatic with fixed potential on electrodes for over 2 hours (Fig. 10).
Fig. 10 shows the behavior of titanium polarized from the OCP (Open Circuit Potential) to 20V with a sweep rate of 0.5Vs−1 in phosphoric acid solutions of different concentration (1M, 2M and 3M H3PO4) containing 0.4 wt.% HF. Flat polarization curve confirm passive behavior of titanium anodized in 2 and 3M H3PO4+0.4 wt.% HF, contrary to current transients recorded in 1M H3PO4+0.4 wt.% HF. The increase of current with potential in that region usually can be explained by the presence of some pores . Polarization curves for more concentrated phosphate solutions show the presence of the anodic peaks, which can be ascribed to the oxygen evolution  followed by a broad passive region. By fixing the concentration of HF (fixing the dissolution rate) the decrease of current with potential indicates that oxide formation dominates over oxide dissolution at relatively higher field strengths or/and passive layer of phosphates is formed over nanotube titania in 2 and 3M H3PO4+0.4 wt.% HF solutions.
Anodic titania nanotubes formed on titanium in 1-3 M H3PO4 with 0.4% wt. HF (Fig. 11) show the morphology of nanotubes on titanium which differ in diameter and the layer thickness due to electrolyte concentration.
SEM observations (Fig. 11) confirmed formation of a highly organized nano-sized pores, ranging from 90 to 120 nm in all applied electrolytes. As apparent, the average nanotube diameter is slightly affected by the supporting electrolyte concentration. Also, the increase of the latter from 1M to 3M under fixed HF concentration results in significant decrease of nanotube layer thickness, from 760±35 nm to 590±35 nm, respectively.
The XPS analysis (Fig. 12) revealed that the highest amount of fluorides in oxide surface layer was obtained in 1M H3PO4+0.3% wt. HF, but in this case the lowest amount of phosphates adsorbed above nanotubes was observed. Using higher concentrations of phosphoric acid 2-3 M H3PO4 Judging on the results of XPS analysis, the competition between fluorides and phosphates is observed during anodizing and the higher concentration of the latter is responsible for higher bioactivity of nanotubes formed in 2M H3PO4+0.4% wt. HF .
The XPS spectra (Ti 2p, O 1s, P 2p and F 1s) revealed that nanotube layers consist of Ti, O, F and P species. The Ti 2p spectra for all samples showed only one doublet line. The position of the Ti 2p3/2 peak on the binding energy scale at 458.8 eV corresponded to titanium dioxide and Ti IV phosphates . One type of phosphorus was revealed by the P 2p3/2 peak position at 133.3 eV associated to phosphate type species, indicating that species from the electrolyte are indeed adsorbed over the oxide film during anodizing. Oxygen was found to exist in two forms. The binding energies of the O 1s spectra corresponded to hydroxyl groups OH- (531.3eV) and oxygen in oxides O2- (529.9 eV). The presence of fluorine in the surface layer was confirmed by the F 1s spectra of binding energy 684.6 eV associated probably with Ti. As it is shown in Fig. 12 the concentrations of titanium corresponding to titanium dioxide and Ti IV phosphates are nearly the same in all tested samples, whereas the concentrations of the other elements vary with the composition of the anodizing electrolyte. Samples anodized in 1M H3PO4+0.3% wt. HF shows the highest amount of O2- (asTiO2) and fluorides, but the lowest amount of phosphates and hydroxyl ions. It means that previously recommended  conditions of uniform nanotubes formation on titanium implant materials favor titanium oxidation and enhance transport of fluorides in formed titania. The similarly high concentrations of relevant elements (Ti and O), together with the highest amount of phosphates of all controlled, are observed in samples anodized in 2-3M H3PO4+0.4% wt. HF. It indicates that the use of more concentrated phosphate electrolyte leads to the increase of phosphates adsorbed over the surface layer of nanotubes in competition with much smaller and more mobile fluorides.
3.1.1. Competition between phosphates and flurides
This is well known that at anodizing a competition between oxide formation and its dissolution exists, and that HF is the key factor, which causes the production of porous oxide layer. However, there is also a competition between phosphates and fluorides in the process of oxide nanotubes formation. These 2 anions differ in size, charge and rates of diffusion in oxides (Tabl. 2)
The effect of the phosphoric acid concentration in fluoride containing electrolytes on the properties of oxide nanotubes formed at anodizing was characterized by SEM/EDS observations and capacitance characteristics when immersed in simulated body fluids (SBF) in order to predict their behavior as the future coatings on titanium for biomaterial applications .
The values of the OCP (Fig. 13) for nanotubes formed in 1M, 2M and 3M H3PO4, each containing 0.4% wt. HF, measured at 25ºC in SBF solution 1 h after anodizing are -0.140 V, -0.170V and -0.195V (SCE), respectively, indicate the observed earlier  decrease of the OCP of oxide produced in more concentrated phosphoric acid solution.
The impedance spectra for titanium anodized in 1-3M H3PO4+0.4% wt. HF were obtained at the OCP for frequency ranging from 105 to 0.18 Hz with ac amplitude 10 mV. The spectra recorded 1 hour after immersion in SBF solution show that variations in chemical composition of the surface layer over obtained nanotubes are confirmed by variations in capacitance characteristics.
The results of EIS tests (Fig.14) indicate nearly the same properties (similar impedance and –θ angle values) for ohmic resistance of the electrolyte and its penetration through nanotube films. However in the low frequency range, the impedance values are sensitive to the
phosphate concentration in anodizing electrolyte, accounting for the processes at the nanotube layer/electrolyte interface which can be associated with deposited products. As the changes between spectra occurred during the first hour of exposure to SBF solution, one can assume that the deposition processes on nanotube layers formed in 2-3M H3PO4+0.4% wt. HF are quick.
The formation of porous metal oxides, ie. titania and alumina, is explained by a field-enhanced model [11,61,63,69] that depends on the ability of ions to diffuse through the metal oxide. Thus, due to the large size the incorporation of the phosphate ions is difficult, but the increased fluoride concentration in solution leads to its ability to migrate and intercalate into the oxide films during the anodizing . The XPS results show (Fig. 12), that the increased fluoride concentration is accompanied by the decreased phosphates and hydroxyl ions in adsorbed layer over nanotubes . It correlates very well with the results of titanium anodizing in electrolyte not containing fluorides, where the gel-like protective layer of phosphates was formed over the oxide .
3.2. Nanotubes on implant alloys in phosphate solutions
Titanium and its implant alloys, mainly ternary alloys of Ti-6Al-7Nb or, are widely used in biomedical implants and dental fields due to their unique mechanical, chemical properties, excellent corrosion resistance and biocompatibility [71 -73].
Further improvement of the unique properties of nanotube anodic layers for medical applications, particularly for enhancement of bone in-growth  and biosensing  require not only the development of the formation method on two phase titanium alloys, but also providing the proper morphology and structure. Reported efforts to form anodic nanotube layers on Ti alloys such as Ti-6Al-7Nb, TiAl , or Ti45Nb  showed the formation of highly inhomogeneous surfaces due to selective dissolution of the less stable phase and/or different reaction rates of the different phases of the alloys.
Studies on development of nanotubes growth on the Ti6A4V  and Ti-6Al-7Nb alloys  were focused on varying the HF concentrations in the phosphoric acid media, in order to establish the pore size distribution and estimate the critical scan rate/concentration ratio for the initiation of nanopitting in compact oxide layer, which would be decisive for the formation of uniform nanotubes on both two-phase alloys.
Among several parameters influencing the quality of nanotubes formed anodically, such as potential, time of anodizing, fluoride ions concentration and scan rate of polarization, particularly the last two seem to be determiners for nanotubes structure and morphology. As an example to show the effect of fluoride ions concentration on the morphology of nanotubes on the implant alloy, the anodizing of the two phase (α+β) Ti6Al7Nb alloy samples in 1 M H3PO4 containing 0.2%; 0.3% and 0.4 % wt. HF to 20V using scan rate 500mV/s and then holding them at that potential for further 2h in the same electrolyte, was performed. Nanotubes of diameter ranging from 50nm to 80nm, with thicker walls over β-phase grains than over α- phase grains, were obtained. During the formation process, which includes two stages: the first potentiodynamic and the second potentiostatic (20V), different electrochemical behaviour was observed in electrolytes of various fluoride concentration.
The implant alloy Ti6Al7Nb (Fig 15) of black α phase (hcp) and white β phase (bcc) irregular shape platelets forming variously oriented colonies, with the surface fraction of α and β phases 78% and 22%, respectively, was enriched with aluminum in oxides over α phase and enriched with niobium over β phase anodic nanotubes.
The typical current transients Fig. 16 recorded during the anodizing of the Ti-6Al-7Nb alloy in 1M H3PO4 containing 0.3 wt.% HF are similar to current transients observed during nanotube oxide layers formation on other alloys in other electrolytes . As in previously described process the whole treatment consists of the potentiodynamic polarization from the OCP to 20V with a scan rate of 0.5Vs−1, followed by the potentiostatic polarization at 20V for further 2 h. However, contrary to constant current density increase observed at anodizing of pure Ti [49,50,79], during the potentiodynamic sweep to 20V at the alloy Ti-6Al-7Nb anodizing the current transients show 2 peaks: the first at about 2-3 V due to oxygen evolution and the second at about 4-6V linked probably to Al oxidation. In the potentiostatic stage of anodizing the current density for the alloy decreases until the end of the treatment, while in case of Ti a broad peak is seen at about 900 s of the anodizing (Fig. 16). According to [78,79] the broad peak, typically recorded in the potentiostatic stage of the process, indicates the dissolution of oxide before reaching final balance between both processes: oxide formation and oxide dissolution during nanotubes formation. Such the balance determines a steady-state oxide layer formation stage during anodizing of metals .
Small pits on β phase grains and regular nanotubes on α-phase are observed in 0.2 wt.% HF (Fig. 17a,b). Irregular tubes on β-phase and regular tubes on α-phase grains are seen after anodising in 0.3 wt. % HF (Fig. 17c,d). Both phases are covered with regular nanotubes in case of samples anodised in 0.4 wt. % HF (Fig. 17e,f), but on β- phase nanotube walls are thicker than on α- phase. Fig. 16 illustrates the dissolved oxide over α-phase on Ti6Al4V alloy and bigger size of nanotubes over β- phase in more concentrated phosphoric acid solution.
According to EDS analysis nanotubes formed on the Ti-6Al-7Nb alloy showed that those films are predominately TiO2 with small amounts of Ti2O3, Al or Nb oxides (Table 3). Aluminium and niobium are present in their most stable oxidation states, Al2O3 and Nb2O3. The amount of alloying elements in the nanotube oxide layer was influenced by the underlying metal microstructure, where Nb was present in the β- phase and Al in the α- phase .
In the combined SEM and XPS examinations  (Fig. 17 and 19) the highest intensities for all controlled elements and groups: titanium oxide and titanium phosphates (458.7eV), oxides (530eV), hydroxyl ions (531.6eV), phosphates (133.3eV) and fluorides (648.6eV), clearly confirm that the most advantageous scan rate and electrolyte composition for the formation of uniform nanotube layer on the Ti6Al7Nb alloy, are 0.5Vs-1 during potentiodynamic stage of anodizing in 1M H3PO4 containing 0.3% wt. HF. Interesting is that also the intensity of niobium (207.3eV) in the most stable of the niobium oxides Nb2O5 , increases with fluoride concentration, but seems to reach the limit in these conditions for 0.3% wt. HF (Fig. 19). The highest current density (Fig. 14) is linked to the biggest nanotube diameters, as it was observed in case of pure titanium anodised in the same conditions .
Due to chemical similarity of titanium and niobium [11, 82] electrochemical behaviour of the Ti-6Al-7Nb electrode should be qualitatively similar to that of the titanium and niobium electrodes in the potential range from -1 to 4V (SCE). Electrochemical oxidation of niobium electrode leads to formation of sub-oxides NbO and NbO2 at the OCP, which partly transform into Nb2O5 oxide at 20V, according to the equations 6-7 [83,84]:
The dissolution process of niobium oxide (β-phase) (5) increases with increasing fluoride concentration , so the fluoride concentration is a crucial factor for nanotubes growth on Ti-6Al-7Nb. Structural and metallurgical aspects of the formation of self-organized anodic oxide nanotube layers on alloys are crucial for medical application to the advanced techniques of biological media immobilization which require morphologically uniform surface.
4. Titania layers formed in phosphate solutions for biosensing
The additional advantageous property of phosphate rich compact and nanotubular anodic oxide layers on titanium is its ability to attach enzymes, proteins or biological cells. To test such the possibility in order to apply anodic surfaces for H2O2 biosensing two electrodes were prepared: 1) the first electrode prepared by the electropolymerization of conducting polymer (PANI) on the surface of Ti/TiO2 (compact) electrode , 2) the second electrode was prepared by using titania nanotubes on titanium as a platform of the 3rd generation biosensor . In both cases the HRP (horseradish peroxide) enzyme was immobilized on the sensing surface. By using either cyclic voltammetry or amperometric modes the feasibility and electrochemical parameters for H2O2 monitoring on Ti/TiO2 surface were checked in the simulated body fluid (SBF). Both electrodes were sensitive to H2O2, however the second electrode only in the presence of thionine as the mediator . Two peaks seen on cyclic voltammograms (Fig. 18) for the Ti/TiO2 (nanotube) electrode with immobilized HRP, indicate the sensitivity of the prepared platform to the presence of H2O2 in the analyte.
Titanium surfaces can be modified by electrochemical treatment in the phosphoric acid solutions for better corrosion resistance, improved physicochemical and electrochemical properties and bioactivity. The formation of oxide layers enriched with phosphorus of 30-120nm thick can be formed in 0.5 M H3PO4 at both galvanostatic anodizing current density values varied in the range of 0.1-0.5 Am-2 and potentiostatically at up to 60V giving yellowish layers porous on the surface. Due to the presence of phosphates they are highly bioactive in comparison to oxides formed in other electrolytes and are covered with hydroxyapatite deposists after 9 days in the SBF solution.
Anodizing in more concentrated 2M solutions of the phosphoric acid generates a gel-like film with thickness of about 100nm on titanium. The film, containing a large number of phosphates nuclei, exhibits its effectiveness to activate titanium surface for biomimetic coating of calcium phosphate. The electrochemically treated titanium was able to form uniform Ca–P coatings on titanium after 48 hour immersions in the SBF solution. The treatment is a simple method to generate bioactive metal surfaces, besides other methods such as alkaline treatment applied to titanium implant materials.
Electrochemical treatment in the phosphoric acid solutions with the addition of 0.2-0.4% wt. HF allows to form on titanium and its implant alloys nano-sized pores (nanotubes) in more concentrated phosphoric acid solutions (1-3M). Their morphology, electrochemical properties and chemical composition are in close relation with the anodic polarization parameters and with the concentration of both ions: phosphates and fluorides. The highest amount of fluorides in surface layer is obtained when using 1M H3PO4+0.3% wt. HF, but in this case the lowest amount of phosphates adsorbed above nanotubes is observed. The use of higher concentrations of phosphoric acid (2-3M H3PO4 with 0.4% wt. HF assures the formation of nanotubes containing the high concentration of both bioactivity enhancing elements, fluorides and adsorbed phosphates. The obtained titania nanotubes show the significantly higher bioactivity in vitro during the first hour of immersion in SBF in comparison to barrier titanium oxide.
Depending on fluoride ion concentrations in anodizing electrolyte morphologically different nanotubular layers have been obtained on both phases of two titanium alloys: the Ti6Al4V and the Ti6Al7Nb alloys. Self organized nanotubes grow on both phases (α and α+β) in 1M H3PO4 containing 0.4% wt. HF, though smaller pore size and thicker wall tubes are obtained on the β phase. The electrochemical behavior of both phases of the alloys differs due to fluoride concentrations which is the key parameter in controlling their morphology. Uniform nanotubes are obtained in 2M H3PO4 containing 0.3% wt. HF at scan rate of polarization 0.5Vs-1 during potentiodynamic stage of anodizing. Such conditions assure the highest fluoride and phosphate concentrations in surface layer of nanotubes on titanium and nanotubes containing niobium oxide on the Ti6Al7Nb alloy. Both features promise a proper coating for improved osteoblast cell adhesion on artificial implants and for biosensing.
Funding from the Polish Ministry of Science and Higher Education under the N507 082 31/2009 project and by the National Centre of Research and Development under ERA-NET/MNT/TNTBIOSENS/1/2011 Project is gratefully acknowledged. My thanks go also to Professor Patrick Schmuki of University of Erlangen for inspiration on nanotubes and to my coworkers from the Biomedical Engineering Division at University of Zielona Góra.
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