Comparison of descriptives and internal consistencies of the unidimensional perceptual features. Calculations are based on the total sample (music and speech group,
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The multimodal perception, integration and mental reconstruction of the physical world provide us, amongst other things, with various modality-specific and modality-unspecific features such as colors, timbres, smells, vibrations, locations, dimensions, materials, and aesthetic impressions, which are or can be related to perceived objects and environments. A fundamental issue is the extent to which such features rely on the different modalities and their cooperation. The present study examined and experimentally dissociated the important modalities of hearing and vision by separately providing and manipulating the respectively perceivable information about the physical world, i.e., auralized and visualized spatial scenes. In everyday life, both the egocentric distance to visible sound sources and the size of a surrounding room are important perceptual features, since they contribute to spatial notion and orientation. They are also relevant about artistic renditions and performance rooms, as they relate, for instance, to the concept of auditory intimacy, an important aspect of the quality of concert halls [1, 2, 3]. Accordingly, both the perceived egocentric distance and the perceived room size were investigated, primarily in the context of artistic renditions.
The interaction between hearing and vision occurs in the perception of various features, pertaining for example to intensity [4], localization [5, 6, 7], motion [8, 9, 10], event time [11, 12], synchrony [13], perceptual phonetics [14], quality rating [15], and room perception [16, 17, 18]. Regarding auditory-visual localization and spatial perception, research has focused mainly on horizontal directional localization to date, followed by distance localization, while room size perception has rarely been investigated. Two superior research objectives may be identified in the literature: One objective is the description of human perceptual performance and its dependence on physical cues. Within this context, distance perception was mainly investigated about its
Experimental stimuli may be real objects (e.g., humans, loudspeakers, mechanical apparatuses) that have diverse physical properties and may bear meaning. Otherwise, the investigation of detailed internal mechanisms using behavioral experiments often calls for neutral objects or energetic events with a maximally reduced number of properties and without meaning (e.g., lights, noise) [5]. Criteria for the selection of one of these stimulus categories are essentially the options of stimulus manipulation (e.g., real objects will hardly allow for conflicting stimuli) and the relation of internal and external validity. The advancement of virtual reality provided experimenters with extended and promising options for manipulating complex, naturalistic stimuli. Since the virtualization of real environments is known to affect various perceptual and cognitive features [20, 21, 22, 23], the impact of virtualization has become another prominent research issue.
The perception of distance and room size in the extrapersonal space depends on particular auditory and visual cues provided by the specific scene. Acoustic distance cues are weighted variably and comprise the sound pressure level and the direct-to-reverberant energy ratio [24, 25, 26], spectral attenuation due to air absorption [27], spectral properties due to temporal and directional patterns of reflections of surrounding surfaces [25], as well as spectral alterations due to both near-field conditions and the listener’s head and torso. Interaural level and time differences also appear to play a role, namely in connection with orientations and motions of the sound source and the listener [28, 29, 30].
In real acoustic environments, perceived egocentric distances are known to be compressed above distances of 2 to 7 m [27, 28, 31, 32, 33], hence they are found to be compressed comparably or even more in virtual acoustic environments [32, 34, 35, 36, 37]. However, a largely accurate estimation in high-absorbent and an overestimation in low-absorbent virtual environments were also reported [18, 38].
Acoustic room size cues comprise the room-acoustic parameters clarity (C80, C50) [39, 40, 41], definition (D50) [41], reverberation time (RT) [39, 42, 43], and likely the characteristics of early reflections [39]. In the medium- and large-sized rooms, the perceived room size was shown to be decreased by a binaural reproduction of the acoustic scene compared to listening in situ [40]. A more recent study found, however, that auralization by dynamic binaural synthesis did not affect the estimation of room size [38].
The estimation of the egocentric distance and the dimensions of visual rooms is based on visual depth cues. Common classifications differentiate between pictorial and non-pictorial, monocular and binocular, as well as visual and oculomotor cues. The cues cover different effective ranges: the personal space (0–2 m), the action space (2–30 m) and/or the vista space (> 30 m) [44]. The non-pictorial depth cues comprise three oculomotor cues:
In real visual environments, distances are normally estimated much more precisely and accurately than in real acoustic environments [47]. Beyond about 3 m distances are increasingly underestimated both under reduced-cue conditions [48] and in virtual visual environments, no matter if head-mounted displays or large screen immersive displays are used [38, 49, 50, 51, 52, 53, 54, 55]. However, also largely accurate estimates in virtual visual environments were reported [18, 56]. While the parallax and the observer-to-screen distance [57], as well as stereoscopy, shadows, and reflections [58] were identified to influence the accuracy of distance estimates in virtual visual environments, the restriction of the field of view [59] and the focal length of the camera lens [60] did not take effect. Room size was observed to be overestimated more in a real visual environment than in the correspondent virtual environment [38], as well as underestimated in other virtual visual environments [18].
Turning to acoustic-visual conditions, the experimental combination of acoustic and visual stimuli can be either congruent or divergent regarding positions or other properties. The widely-used variation of the
Under congruent conditions, as experienced in real life, distance estimation is normally highly accurate. Using virtual sound sources and photographs, the additional availability of visual distance information was demonstrated to improve the linearity of the relationship between the physical and the perceptual distance, and to reduce both the within- and the between-subjects variance of the distance judgments [61]. However, virtual acoustic-visual environments may, like virtual visual environments, be subject to compressed distance perception [32], regardless of the application of verbal estimation or perceptually directed action as a measurement protocol [36, 37]. A perceptual comparison between mixed and virtual reality [62] showed that the virtualization of the visual environment increased “aurally perceived” distance and room size estimates (p. 4). The perceived room width was found to be underestimated under the visual, overestimated under the acoustic, and well-estimated under the acoustic-visual conditions [17]. Findings on the accuracy of room size perception are in the same way inconsistent for acoustic-visual environments, as they are for visual environments (see above) [18, 38].
Experiments applying the conflicting stimulus paradigm are normally both more challenging and more instructive [36]. Such experiments have revealed that the localization of an auditory-visual object is largely determined by its visual position, which becomes particularly obvious when compared to the localization of an auditory object. This phenomenon was investigated relatively early [5], and in the case of a lateral or directional offset in the horizontal plane, it was initially referred to as the
In the case of an egocentric distance offset, the phenomenon was initially termed the
Indeed, it has been demonstrated that both visual and acoustic stimulus displacements cause significant changes in egocentric distance estimates [68], indicating that visual and auditory influences occur at the same time, however, with different weights. Regarding auditory features, Postma and Katz varied both visual viewpoints and auralizations in a virtual theater, while asking experienced participants for ratings upon distance and room acoustic attributes [69]. Few attributes (including auditory distance) were significantly influenced by the visual contrasts, whereas most attributes were by the acoustic. Interestingly, a deeper data analysis allowed partitioning participants into three groups being mainly susceptible to auditory distance, loudness, and none of the features, respectively, when exposed to different visual conditions. Amongst others, the study points to the principle, that acoustic and visual information weigh normally highest on auditory and visual features, respectively.
In the course of the advancement of a probabilistic view, it was evidenced that the weights adapt to the reliabilities of the sensory estimates in a statistically optimal manner [70]. Maximum Likelihood Estimation (MLE) modeling was shown to apply to different multisensory localization tasks [47, 71, 72, 73]. Therefore, acoustic-visual stimuli should generally yield a more precise localization than merely acoustic or visual stimuli [72]. The weights may either be experimentally reduced by adding noise to the stimuli, or in turn, if estimated otherwise, indicate the relative acuity of the stimuli and the reliability of their sensory estimates, respectively. For instance, due to missing or largely reduced interaural level difference and interaural time difference cues, auditory positional information has a lower weight in case of a directional or depth offset in the median plane; in this case, localization is therefore more prone to the influence of visual positional information than in the case of a lateral offset [9, 74]. It was found that acoustic and visual contributions are not symmetric about frontal distance: Using LEDs and noise bursts, a “visual capture” effect and a respective aftereffect in frontal distance perception was observed, with a relatively greater visual bias for visual stimulus components being closer than the acoustic components ([75], p. 4).
Combining MLE with Bayesian causal inference modeling [76] is based on the idea that increasing temporal or spatial divergences between sensory-specific stimuli make the perceiver’s inference of more than one physical event more likely, and that multisensory integration takes place only for stimuli subjectively caused by the same physical event. A recent study demonstrated, however, a higher weight of visual signals in auditory-visual integration of spatial signals than predicted by MLE, which might be due to the participants’ uncertainty about a single physical cause [77]. While the result of the causal inference is normally not directly observable, the perceived spatial congruency is: Using stereoscopic projection and wave field synthesis, André and colleagues presented participants with 3D stimuli (a speaking virtual character) containing acoustic-visual angular errors. As expected, a higher level of ambient noise (SNR = 4 dB A) caused a 1.1° shift of the point of subjective equivalence and a steeper slope (−0.077 instead of −0.062 per degree) of the psychometric function. Results were not statistically significant, arguably due to the still too high SNR [78].
Evaluating different variants of probabilistic models through experiments using a virtual acoustic-visual environment and applying a dual-report paradigm, the Bayesian causal inference model with a probability matching strategy was found to explain the auditory-visual perception of distance best [79]. The authors also calculated the sensory weights for visual and auditory distances and found that in windows around the correspondent physical distance, auditory distances were predominantly influenced by visual, while visual distances were slightly influenced by auditory sensory estimates. Visual-auditory weights ranged from 0 to 1, auditory-visual weights from 0 to 0.2. Another study showed a major influence of the acoustic properties of spatial scenes on the collective egocentric distance perception (probably due to a substantially restricted visual rendering), whereas room size perception predominantly relied on the visual properties. The virtual environment was based on the dynamic binaural synthesis, speech and music signals, stereoscopic still photographs of a dodecahedron loudspeaker in four rooms, and a 61″ stereoscopic full HD monitor with shutter glasses [18].
The cited studies applied different data collection methods (e.g., triangulated blind walking, absolute scales, 2AFC), virtualization concepts (no virtualization, direct rendering, numerical modeling), stimulus content types (e.g., speech, noise; LEDs, visible sound sources), visual moves (photographs, videos), stimulus dimensionalities (2D, 3D), and reproduction formats (e.g., monophonic sound, sound field synthesis; head-mounted displays, large immersive screens). Thus, connecting the results in a systematic manner is challenging. Findings on the influences of concrete physical properties on percepts and their parameters have not achieved consistency.
Following a research strategy from the general to the specific, the present study focuses on the influences of the acoustic and visual environments’ properties in their totality. To this end, whole rooms and source-receiver configurations were experimentally varied. To make this feasible, a collective instead of an individual testing approach was taken, i.e., identical test conditions were allocated not to different repetitions (as necessary for data collection in the context of probabilistic modeling) but to different participants. To emphasize external validity and step towards “naturalistic environments” ([65], p. 805), two prototypic types of content (music, speech), six physically existing rooms, direct 3D renderings, long and meaningful stimuli, and a perceptually validated virtual environment were applied.
Methodologically, the prominent co-presence paradigm entails two restrictions. Firstly, the comparison between the acoustic or visual and the acoustic-visual condition involves two sources of variation: (a) the change between the stimulus’ domains (acoustic vs. visual), and (b) the change between the numbers of stimulus domains (1 vs. 2)—i.e., between two basic modes of perceptual processing. Thus, the co-presence paradigm confounds two factors at the cost of internal validity. Since single-domain (acoustic, visual) stimuli do not require a multimodal trade-off, whereas multi-domain (acoustic-visual) stimuli do, different weights of auditory and visual information depending on the basic mode of perceptual processing are expected [79]. To take account of the sources of variation, two dissociating research questions (RQs) were posed.
As a second restriction, the co-presence paradigm does not cover variations within the multi-domain stimulus mode, though it is prevalent in everyday life. Hence, additional RQs ask for the effects of the
RQ 1: To what extent do the perceptual estimates depend on the stimulus domain (acoustic vs. visual, and thereby of the involved modality) as such?
H10: μA = μV.
RQ 2: To what extent do the perceptual estimates depend on the basic mode of perceptual processing (single vs. multi-domain stimuli)?
H20: 2 · μAV = μA + μV.
RQ 3: To what extent do the perceptual estimates depend on the complex acoustic properties of the multi-domain stimuli?
H30: μA1V• = μA2V• = μA3V• = μA4V• = μA5V• = μA6V•.
RQ 4: To what extent do the perceptual estimates depend on the complex visual properties of the multi-domain stimuli?
H40: μA•V1 = μA•V2 = μA•V3 = μA•V4 = μA•V5 = μA•V6.
RQ 5: To what extent do the perceptual estimates depend on the interaction of the complex acoustic and visual properties of the multi-domain stimuli?
H50: μA
Note that not only distance and room size cues but whole scenes were varied, to infer the effects of the entire physical properties of the performance rooms, and therefore of the sensory modalities as such in the context of these environments. RQs 3–5 were made comparative by asking to which extent acoustic and visual properties, and their interaction, do proportionally account for the estimates. For this purpose, commensurable ranges of the factors had to be ensured (2.3, 2.7).
Dependent variables were the perceived egocentric distance and the perceived room size. Where reasonable, the accuracy of the estimates about the physical distances and sizes was also considered.
Answering RQs 1 to 2 requires the application of the co-presence design paradigm. Auralized, visualized, and auralized-visualized spatial scenes are levels of one factor. Answering RQs 3 to 5 requires the acoustic and visual properties of the scenes to be independent factors rather than just levels of one factor, i.e., the application of the conflicting stimulus paradigm. To allow for the quantification of the proportional influences of acoustic properties, visual properties, and their interaction on the perceptual features, however, certain methodological criteria have to be met, because light and sound cannot be directly compared due to their different physical nature. In particular, not only spatiotemporal congruency but also
These considerations result in the need for preservation of all perceptually relevant physical cues and a direct rendering, which we distinguish from fully numerical or partly numerical (hybrid) simulations. The latter approaches are based on assumptions of the physical validity of parametrized material and geometrical room properties, the imperceptibility of structural resolution limits, and/or the physical validity of the applied models on sound and light propagation, including methods of interpolation. By using the term direct rendering, we indicate that the rendering data corresponding to all supported participants’ movements were acquired in situ, i.e., neither calculated from a numerical 3D model nor spatially interpolated (see 2.5.).
With the objective of a clear description of investigated effects, it is indicated to factually and terminologically differentiate between ontological realms (
In view of both the context of the study (artistic renditions, performance rooms) and the complex variation of the stimuli (2.1), the collection of values of various features was of interest. Accordingly, a differential was used. A superordinate objective of the research project is a comparison of the features regarding their respective dependencies on the presences and properties of the acoustic and visual stimuli. Hence, the questionnaire consisted of 21 perceptual features, subdivided into four sets: auditory features (e.g.,
Since the visual stimuli showed only a part of the frontal hemisphere (see 2.5), the participants had to base their assessment of the invisible rear part of the rooms’ length on the visible frontal length, the room shape, their position in the room, and their experiential knowledge on the shape and size of performance rooms. Hence, before analyzing the calculated room volume/size estimates, dispersion and reliability measures of the unidimensional perceptual features were inspected (Table 1).
Measure | ||||
---|---|---|---|---|
Mean | 45.833 | 22.162 | 14.672 | 10.431 |
Standard error of mean | 0.905 | 0.542 | 0.229 | 0.185 |
Cronbach’s Alpha | 0.926 | 0.889 | 0.867 | 0.850 |
Comparison of descriptives and internal consistencies of the unidimensional perceptual features. Calculations are based on the total sample (music and speech group,
Neither the reliability nor the dispersion of the perceived length is conspicuous, since the values for Cronbach’s Alpha are throughout high, for the perceived length even excellent, and the error-to-mean ratios are consistent across the perceptual features. By calculating the cube root of the product of the three collected features, the one-dimensional feature
Since answering RQs 1 to 2 requires the application of the co-presence paradigm, the factor
Label | KH | RT | KO | JC | KE | GH |
---|---|---|---|---|---|---|
Volume | 1899 | 1903 | 7266 | 8079 | 19539 | 22202 |
Size | 12.383 | 12.392 | 19.369 | 20.066 | 26.934 | 28.106 |
Position of receiver (row no./seat no.) | 6/8–9 | 11/178 | 9/20 | 3/- | -/- | 6/9 |
Distance receiver—central source | 9.97 | 9.90 | 9.46 | 7.19 | 15.84 | 9.84 |
Absorption coefficient | 0.18 | 0.20 | 0.30 | 0.17 | 0.02 | 0.28 |
Reverberation time | 1.29 | 0.80 | 1.31 | 2.81 | 7.92 | 2.29 |
Early Decay Time | 1.31 | 0.72 | 1.17 | 2.67 | 8.20 | 1.99 |
Geometric and material properties of the selected rooms (taken from [85]). The index
The number of trials within a test sequence corresponds to the number of experimental conditions (factor level combinations). There are two options for allocating the trials to the scale items: (a) A long stimulus (ca. 2:00 min, cf. 2.5) is judged by means of the 21 items (2.2); there is just one test sequence. (b) A short stimulus (ca. 6 sec) is judged by means of one feature; the number of test sequences corresponds to the number of features. Option (a) was chosen for the following reasons: (1) In the case of option (b), the comparison of the features, as required by the research project (2.2), would be confounded with the repetition of a stimulus, including greater time intervals, whereas it is not in case of option (a). (2) Short stimuli would run counter to both the context (1.1) and the methodological aim (2.2, 2.3) of the study: artistic renditions are much longer than a few seconds, and—particularly regarding the aesthetic and presence features—responses to very short extracts could not be generalized for entire renditions. (3) To yield valid responses, stimuli must provide enough time and information for judgment formation. Building up an aesthetic impression about very short extracts of an artistic rendition would be hardly possible due to the lack of information about the course of time. Thus, artistically self-contained sections were to be presented at least. Long stimuli provide a greater number and variety of physical events, so that each participant can rely on the individually most helpful cues. (4) In the case of option (a) the decision times vary and are unknown, i.e., within the samples, decision times, as well as causal events and their cues, are pooled. On the one hand, this increases the external validity. On the other hand, it also decreases the internal validity, though, to an acceptable level, since both physical distance and size are constant within each stimulus, and attribution of the estimates to detailed cues or events is not part of the research questions (cf. 1.3).
The required sample size was calculated a priori with the aid of the software package G*POWER 3 [85, 86]. Since the groups of the factor
Hypoacusis; criterion: audiogram, hearing threshold >20 dB HL at either ear at any of seven tested frequency bands (125 to 8000 Hz), uncompensated by hearing aid (0 subjects).
Vision deficits; criterion: self-reported deficits, uncompensated by visual aid (0 subjects).
Red and/or green color blindness; criterion: unpassed Ishihara tests for protanomaly and deuteranomaly (3 subjects).
Loss of stereopsis; criterion: unpassed contour stereopsis test using the shutter glasses of the projection system (4 subjects).
Technical incident; failure of saving response data (6 subjects).
Subjectively untrue responses; criterion: implausible perceptual bias (factor ≥ 5) with reference to visual geometric dimensions (14 subjects, most frequent response: “0 m”).
The resultant valid net sample sizes accounted for
As far as possible in a virtual environment, a maximum ecological validity of the stimuli was sought by selecting dedicated performance rooms, artistic content and professional music and speech performers.
Six performance rooms differing in volume (low, medium, high) and average acoustic absorption coefficient (low: αmean(Sabine) < 0.2; high: αmean(Sabine) ≥ 0.2) were selected. Taking into account good speech intelligibility and an accurate perceptibility of the physical room properties (e.g., the visibility of the ceiling height), optimum receiver positions were defined. Based on geometric measures acquired in situ, models of the interior spaces, including the source-receiver-arrangements, were built using the software
The artistic content comprised a musical work and a text, which were chosen to support the perceptibility of the specific room properties by featuring, e.g., impulsivity and sufficient pauses. Two-minute excerpts of Claude Debussy’s String Quartet in g minor, op. 10, 2nd movement, and of Rainer Maria Rilke’s 1st Duino Elegy were selected. The artistic renditions were audio recorded in the anechoic room of the Technische Universität Berlin.
The performances were presented in the Virtual Concert Hall at Technische Universität Berlin, providing virtual acoustic and visual 3D renditions in rooms. It was particularly designed to meet the methodological requirements (2.1, 2.3), and was completely based on directional binaural room impulse responses (BRIRs) and stereoscopic panoramic images acquired in situ by means of the head and torso simulator
Participant in the Virtual Concert Hall (visual condition: KO).
The used BRIRs contained the fixed HRTFs of
The virtual environment did not provide auditory motion parallax cues by supporting lateral motion interactivity and rendering. This was due to limited in-situ acquisition times in the performance rooms. It would have required measurements at several additional positions of the head and torso simulator, depending on the content-specific minimum audible BRIR grid [101, 102], and thus would have multiplied the expenditure of acquisition time beyond the rooms’ availability. However, auditory motion parallax, describing the change in the angular direction of a distant sound source due to the movement of the listener, is assumed to be a supporting cue in absolute distance estimation [103] and known to be a cue in relative depth estimation [104]. Regarding a distance range within the personal space, it was demonstrated by means of a depth discrimination task, and under exclusion of all other distance cues, that auditory motion parallax is exploited by listeners allowing for the perception of distance differences of unknown acoustic stimuli [104]. The cue was shown to be effective for distances between 0.3 and 1.0 m and to be exploitable for lateral head movements within a range of 46 cm. The participants’ sensitivity was highest during self-induced motion. Even sensitive subjects did not perceive distance differences corresponding to angular displacements below 3.2°. This value is higher than the minimum audible movement angles (MAMAs) found in previous research (see [105] for an overview). Regarding a distance range of 1 to 10 m, Rumukkainen and colleagues determined the self-translation minimum audible angle (ST-MAA) to be 3.3° by means of 2AFC discrimination tasks without an external reference [106]. Taking into account the absence of external references in the present study and applying the ST-MAA to the nearest sound source used (7.19 m), a concertgoer would remain below perceptual threshold within a lateral moving range of ±41.5 cm, which corresponds to 150% of a typical concert seat’s width. Respective lateral movements are normally not observed amongst visitors of classical concerts. Since a relative lateral shift of the listener above the perceptual threshold is a precondition for yielding distance information from the auditory motion parallax cue by triangulation, we do expect neither an appreciable bias nor a deterioration of the accuracy of distance perception introduced by the absence of lateral motion interactivity and rendering.
As a result, the Virtual Concert Hall at Technische Universität Berlin provided almost all relevant auditory cues without major biases (rich-cue condition). Exceptions are the missing supports for (rarely performed and normally small) head orientations around the pitch and roll axes.
The sound pressure level of the virtual rendition was adjusted to the sound pressure level of a live rendition of a string quartet in a real room, which was recorded by the calibrated head and torso simulator. Accounting for the gain of the signal chain and the rooms’ STI measures, the level of the scenes’ average sound pressure level at the blocked ear canal was
The acquisition of the visual rendering data applied a fixed stereo base, which does not necessarily accord with the participants’ individual interpupillary distances (IPDs). Respective differences might potentially bias the individual distance and room size perception. To date, experimentation has shown inconsistent effects of the variation of IPD differences on distance perception (see [46] for a review). Most studies cannot be translated into the present study, since they investigated maximum target distances of 1 m and/or used simple numerically modeled objects/environments. Moreover, results differ regarding the significance, the size and/or the direction of the effects. This is apparently due to different rendering technologies (stereoscopic projection, HMD, CAVE), stages of virtualization (mixed reality, virtual reality), target distances (personal space, action space), simulated objects/environments (simple graphic objects, shapes, persons in hallways), and measurement protocols (triangulated distance estimation, blind walking, visual alignment, verbal estimation) [107, 108, 109, 110, 111, 112, 113]. Few experiments investigated distances roughly similar to those used in the present study (about 7 to 16 m). While Willemsen and colleagues did not observe a significant effect of IPD individualization on distance judgments [114], a large variation of the stereo base (0 to 4 times the IPD) showed significant effects on both distance and size judgments: Greater stereo bases resulted in perceptually closer and smaller objects [115]. However, relevance for the descriptive measures, effect sizes and significances of the present study is given rather by the expected value and distribution of the IPD differences than by their individual values. Anthropometric data of the German resident population, from which the sample was drawn, state median IPDs of 61 mm (male persons) and 60 mm (female persons) within the age range of 18 to 65 years [116]. Since the values do nearly exactly meet the stereo base of the target acquisition (60 mm), a substantial collective perceptual bias is unlikely to occur.
Limitations of the visual rendering pertain to the field of view (161° × 56°), which should at least not affect distance perception [59, 117]; the angular resolution (2.1 arcmin), which might affect distance perception [57]; the fixed single focal plane in stereoscopy providing an invariant accommodation cue, so that the connection between convergence and accommodation is suspended [45]; and an undersized luminance of the projection. Data projectors could not provide the luminance and the contrast of the real scenes, especially in connection with shutter glasses. Thus, the luminances of the scenes were fitted into the projectors’ dynamic range while maintaining compressed relations of the luminances. Scene luminances were calculated from exposure time, aperture, and ISO arithmetic film speed of correctly exposed photographs of a centrally placed and vertically oriented 18% gray card according to the additive system of photographic exposure (APEX). The average loss of the luminance value
Since electronic media transform both the physical stimuli and their perception, the replacement of natural by mediatized stimuli for serious experimental purposes demands the knowledge of the perceptual influences of the applied mediatizing system, as also pointed out by [16, 21]. The rendering technique of the Virtual Concert Hall was shown to provide perceptually plausible auralizations [119]. Specifically, the Virtual Concert Hall at Technische Universität Berlin was subjected to a test of auditory-visual validation by comparing a real scene and the correspondent virtual scene [38]. Amongst others, it yielded nearly equal loudness judgments of the real and the virtual environment, whereas the virtual environment—apparently due to the dark surrounding—was perceived slightly brighter than the respective real environment. The virtualization also generally lowered the perceived source distance and the perceived size of a real room—mainly due to the visual rendering. The mere auditory underestimation of source distance and room size introduced by the virtualization amounted only to 6.6 and 1.9%, respectively. The biases are considered in the discussion section.
Each participant ran through the test procedure individually. The procedure lasted about 3 hours and 10 minutes, and comprised color vision and stereopsis tests, audiometry, a socio-demographic questionnaire, a privacy agreement, the clarification of the questionnaire, the measurement of the individual inter-tragus distance (necessary for the technical adaption to the individuals’ ITDs), cabling, a familiarization sequence, and the actual test runs, inclusive of self-imposed breaks.
Arithmetic means standard deviations (Tables 11 and 12) and standard errors were calculated for all combinations of factor levels. The means were plotted against the combinations. According to the test design (2.3), the co-presence paradigm required 3 × 6 repeated measures analyses of variance (rmANOVA), the conflicting stimulus paradigm 6 × 6 rmANOVA for either level of
Source distance showed significant main and interaction effects of
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1521.061 | 1.782 | 853.503 | 36.965 | <0.001 | 0.086 | 0.306 | 0.122 | 0.430 | >0.999 | |
Error ( | 2016.285 | 87.325 | 23.090 | |||||||
4610.610 | 3.845 | 1199.166 | 137.464 | <0.001 | 0.260 | 0.593 | 0.296 | 0.737 | >0.999 | |
Error ( | 1643.487 | 188.397 | 8.724 | |||||||
597.939 | 7.113 | 84.059 | 9.593 | <0.001 | 0.034 | 0.187 | 0.052 | 0.164 | >0.999 | |
Error ( | 3054.229 | 348.552 | 8.763 |
Results of the rmANOVA for
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
655.466 | 1.683 | 389.381 | 23.350 | <0.001 | 0.058 | 0.248 | 0.073 | 0.387 | >0.999 | |
Error ( | 1038.621 | 62.284 | 16.676 | |||||||
1712.901 | 3.387 | 505.745 | 41.676 | <0.001 | 0.152 | 0.423 | 0.171 | 0.530 | >0.999 | |
Error ( | 1520.729 | 125.315 | 12.135 | |||||||
639.600 | 5.709 | 112.027 | 10.836 | <0.001 | 0.057 | 0.245 | 0.072 | 0.227 | >0.999 | |
Error ( | 2183.952 | 211.245 | 10.338 |
Results of the rmANOVA for
Means (markers) and standard errors (bars) of
Means (markers) and standard errors (bars) of
Regarding RQ 1, a priori main contrasts indicate that the mean estimates at level
Looking at the accuracy of the estimates, the mean estimates differed from the mean physical source distance by −2.36 m (−22.7%) at level
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
469.724 | 5.000 | 93.945 | 13.143 | <0.001 | 0.017 | 0.133 | 0.023 | 0.211 | >0.999 | |
Error ( | 1751.252 | 131.608 | 13.307 | |||||||
6256.608 | 2.602 | 2404.324 | 105.444 | <0.001 | 0.233 | 0.551 | 0.238 | 0.683 | >0.999 | |
Error ( | 2907.446 | 127.509 | 22.802 | |||||||
134.833 | 13.677 | 9.858 | 1.566 | 0.086 | 0.005 | 0.071 | 0.007 | 0.031 | 0.868 | |
Error ( | 4219.961 | 670.192 | 6.297 |
Results of the rmANOVA for
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
375.912 | 1.667 | 225.526 | 9.314 | 0.001 | 0.023 | 0.153 | 0.028 | 0.201 | 0.951 | |
Error ( | 1493.259 | 61.672 | 24.213 | |||||||
2936.460 | 2.724 | 1077.931 | 48.375 | <0.001 | 0.178 | 0.465 | 0.183 | 0.567 | >0.999 | |
Error ( | 2245.993 | 100.794 | 22.283 | |||||||
31.620 | 12.609 | 2.508 | 0.531 | 0.902 | 0.002 | 0.044 | 0.002 | 0.014 | 0.317 | |
Error ( | 2203.942 | 466.540 | 4.724 |
Results of the rmANOVA for
Figures 4 and 5 show the generally lower mean distance estimates for the speech by trend. The figures also illustrate the ranges of the mean estimates. The average range of mean estimates caused by
Means (markers) and standard errors (bars) of
Means (markers) and standard errors (bars) of
Room size showed significant main and interaction effects of
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
10148.965 | 1.651 | 6145.611 | 70.421 | <0.001 | 0.115 | 0.361 | 0.180 | 0.590 | >0.999 | |
Error ( | 7061.808 | 80.919 | 87.270 | |||||||
28109.442 | 3.650 | 7701.183 | 226.890 | <0.001 | 0.319 | 0.685 | 0.379 | 0.822 | >0.999 | |
Error ( | 6070.632 | 178.851 | 33.942 | |||||||
3733.981 | 7.522 | 496.424 | 21.814 | <0.001 | 0.042 | 0.210 | 0.075 | 0.308 | >0.999 | |
Error ( | 8387.358 | 315.667 | 26.570 |
Results of the rmANOVA for
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
6484.837 | 1.513 | 4285.200 | 42.093 | <0.001 | 0.082 | 0.299 | 0.131 | 0.532 | >0.999 | |
Error ( | 5700.224 | 55.992 | 101.803 | |||||||
26573.103 | 2.994 | 8875.557 | 165.259 | <0.001 | 0.337 | 0.713 | 0.383 | 0.817 | >0.999 | |
Error (R | 5949.496 | 101.789 | 58.449 | |||||||
3000.770 | 6.785 | 442.292 | 19.791 | <0.001 | 0.038 | 0.199 | 0.065 | 0.348 | >0.999 | |
Error ( | 5610.150 | 251.030 | 22.349 |
Results of the rmANOVA for
Means (markers) and standard errors (bars) of
Means (markers) and standard errors (bars) of
Regarding RQ 1, a priori contrasts indicated that the mean estimates at level
As with source distance, the range of the mean room size estimates introduced by the factor
Accuracies were generally low regardless of the level of
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3275.179 | 2.048 | 1599.570 | 25.911 | <0.001 | 0.024 | 0.156 | 0.031 | 0.346 | >0.999 | |
Error ( | 6193.742 | 100.329 | 61.734 | |||||||
32107.238 | 3.203 | 10025.415 | 110.275 | <0.001 | 0.233 | 0.551 | 0.239 | 0.692 | >0.999 | |
Error ( | 14266.617 | 156.927 | 90.913 | |||||||
375.257 | 12.004 | 31.262 | 1.344 | 0.189 | 0.003 | 0.052 | 0.004 | 0.027 | 0.754 | |
Error ( | 13678.450 | 588.172 | 23.256 |
Results of the rmANOVA for
S. o. V. | 1-β | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
3799.130 | 1.446 | 2626.800 | 11.517 | <0.001 | 0.026 | 0.162 | 0.030 | 0.237 | 0.968 | |
Error ( | 12205.465 | 53.513 | 228.084 | |||||||
23087.978 | 2.228 | 10363.307 | 54.821 | <0.001 | 0.155 | 0.429 | 0.160 | 0.597 | >0.999 | |
Error ( | 15582.628 | 82.431 | 189.039 | |||||||
185.804 | 7.540 | 24.642 | 0.662 | 0.716 | 0.001 | 0.035 | 0.002 | 0.018 | 0.296 | |
Error ( | 10382.097 | 278.982 | 37.214 |
Results of the rmANOVA for
Figures 8 and 9 show the generally lower mean room size estimates for the speech by trend. The figures also illustrate the ranges of the mean estimates. The average range of mean estimates caused by
Means (markers) and standard errors (bars) of
Means (markers) and standard errors (bars) of
Most of the results apply likewise to both egocentric distance and room size estimation. RQ 1 asked for the difference between the modalities as such. Mean estimates across the rooms based only on visual information significantly and considerably exceeded those based only on acoustic information, specifically by about a fourth of the mean physical property in the case of distance and by about a third in the case of size. Hence, H11 can be accepted and might be reformulated directionally (H11: μA < μV) for future experimentation. Regarding egocentric distance estimation, the finding is plausible in principle given the reported compression of distance perception in real acoustic environments [27, 28, 31, 32, 33] and virtual acoustic environments [32, 34, 35, 36]. However, it does not agree with [36], who observed a compressed perception of visual distances between 1.5 and 5.0 m, or with [18], who used nearly the same auralization system in connection with smaller distances (1.93–5.88 m) and a restricted visualization. Though the general finding
Regarding RQ 2, there is evidence that the basic mode of perception (processing of single- vs. multi-domain stimuli) as such alters perceptual estimates of geometric dimensions in virtual rooms. Mean estimates based on acoustic-visual stimuli did not equal the average of the mean estimates based on either only acoustic or only visual stimuli. Rather, mean estimates of source distance under the acoustic-visual condition (with acoustic-visually congruent stimuli) were located at 85% (music) of the range between the mean estimates of the levels
Considering the multi-domain mode of perception and applying the conflicting stimulus paradigm, the distance and size estimates depended significantly on both the acoustic and the visual properties of the stimuli (RQs 3 and 4). Generally, about 89% of the explained variance arose from the entire visual and 10% from the entire acoustic information provided by the virtual environment. For both egocentric distance and room size perception, acoustic information showed a slightly greater proportion of explained variance under the speech than under the music condition.
In accordance with the MLE modeling of auditory-visual integration in principle, the acoustic and visual proportions of the explained variance appear to vary strongly according to the availability and, respectively, the richness of the cues in the particular domains: A preliminary experiment under substantially restricted visualization conditions (reduced field of view, reduced spatial resolution, still photographs instead of moving pictures, no maximal acoustic-visual congruency due to visible loudspeakers as sound sources) and non-restricted auralization conditions (identical auralization system) yielded a reversed order of proportions of the explained variance (cf. 2.7), which amounted to 33% for factor
Against the background of the prevalent term
Since the involved modalities and the mode of perception were constant across all factor levels, it may be assumed that VR-induced biases apply likewise to all factor levels of the conflicting stimulus paradigm and their combinations. Hence, the findings on RQs 3 to 5, i.e., the inferential statistics and the
Within the test design, the presence and properties of the acoustic and visual domains were varied to experimentally dissociate the auditory and the visual modalities. Because this variation was categorical, i.e., comprising the entire
There were some additional results on factors and measures which were not explicitly asked for by the RQs:
Both egocentric distance and room size mean estimates, regardless of whether based on acoustic, visual or acoustic-visual stimuli, were obviously lower for speech than for music (though this was not hypothesized or tested, see 2.7). Hence, there is a reason for hypothesizing an influence of content type. This might be due to differences between music and speech regarding, e.g., the bandwidth and energy distribution of the frequency spectra carrying spatial information, perceptual filtering and processing, receptiveness, and/or experiential geometric situations (non-mediatized speech is normally received from lower distances and within smaller rooms than non-mediatized music).
Both the non-significant interaction effect and the particular mean estimates in the experiment according to the conflicting stimulus paradigm indicated that acoustic-visual (mainly spatial) congruency of the stimulus properties did not lead to minimum, maximum or especially accurate mean estimates. This observation is not apt to constitute a general hypothesis, since congruency might play a greater role by contrast with a greater range of the incongruencies (e.g., further-away sound sources) or a greater number of incongruent properties (e.g., including incongruent content).
Egocentric distance mean estimates were most accurate under the acoustic-visual (music) and visual (speech) condition; the room size mean estimates, which were generally inaccurate, likely due to the lack of the visual rendering of the rooms’ rear part, were most accurate under the acoustic condition. In contrast to previous studies [32, 36], regardless of general under- or overestimations of the geometric properties (
Looking at the conflicting stimulus paradigm, the minimum and maximum mean estimates of both source distance and room size did not consistently correspond to the minimum and maximum physical distances and sizes.
Because mean estimates based on purely acoustic stimuli were generally higher in low-absorbent than in high-absorbent rooms (cf. [18]), the range of mean estimates introduced by the factor
Observations (d) and (e) and differences between the studies regarding domain proportions (4.3) give reason to hypothesize that structural and material properties of rooms influence distance perception. Thus, an additional experimental dissociation of the factors physical source distance, physical room size, and acoustic absorption (all else being equal) might be instructive. Furthermore, more detailed physical factors affecting both the acoustic and the visual domain might be disentangled (primary structures, secondary structures, materials). Because of the trade-off between the requirement of ecological stimulus validity and the costs of stimulus production, it might be worth investigating the moderating effects of certain aspects of virtualization (direct rendering, stereoscopy, visually moving persons). In the future, one major aim of research into the perception of geometric properties might be the connection of the modeling of internal mechanisms and the physical-perceptual modeling.
The influence of the presence as well as of the properties of acoustic and visual information on the perceived egocentric distance and room size was investigated applying both a co-presence and a conflicting stimulus paradigm. Constant music and speech renditions in six different rooms were presented using dynamic binaural synthesis and stereoscopic semi-panoramic video projection. Experimentation corroborated that perceptual mean estimates of geometric dimensions based on only visual information considerably exceeded those based on only acoustic information in general. However, the perceptual mode as such (single- vs. multi-domain stimuli) altered the perceptual estimates of geometric dimensions: Under the acoustic-visual condition with acoustic-visually congruent stimuli, the presence of visual geometric information was generally given more weight than the presence of acoustic information. While the egocentric distance estimation under the acoustic-visual condition did not tend to be compressed for music, it did for speech. When only acoustic stimuli were available, the greater amount of acoustic information provided by low-absorbent rooms appeared to be perceptually exploited to improve the accuracy of room size perception. Within the multi-domain mode of perception involving 30 acoustic-visually incongruent and 6 congruent stimuli, auditory-visual estimation of geometric dimensions in rooms relied about nine-tenths on the variation of visual, about one-tenth on the variation of acoustic properties, and negligibly on the interaction of the variation of the particular properties. Both the auditory and the visual sensory systems contribute to the perception of geometric dimensions in a straightforward manner. The observation of generally lower estimates for speech than for music needs to be corroborated and clarified. Further experimentation dissociating the factors source distance, room size, and acoustic absorption (all else being equal) is needed to clarify their particular influence on auditory-visual distance and room size perception.
According to the funding institution (Deutsche Forschungsgemeinschaft) an ethical approval is not required, since the respective indications do not apply [125]. The study was conducted under the ethical principles of the appropriate national professional society (Deutsche Gesellschaft für Psychologie) [126].
This work was carried out as a part of the project “Audio-visual perception of acoustical environments”, funded by the Deutsche Forschungsgemeinschaft (DFG MA 4343/1-1) within the framework of the research unit SEACEN, coordinated by Technische Universität Berlin and Rheinisch-Westfälische Technische Hochschule Aachen, Germany. We thank the staff of the performance rooms for their friendly cooperation, the Berlin Budapest Quartet (Dea Szücs, Éva Csermák, Itamar Ringel, Ditta Rohmann) and actress Ilka Teichmüller for their performances, Alexander Lindau, Fabian Brinkmann, and Vera Erbes for the in-situ acquisition of the rooms’ acoustic and visual properties, Mina Fallahi for the geometric picture editing, Annika Natus, Alexander Haßkerl, and Shamir Ali-Khan for the 3D video shooting and post-production, and all test participants. Finally, we thank the two anonymous reviewers for critically reading the manuscript and suggesting substantial improvements.
The authors have no conflict of interest to declare.
Measure | off | 1 | 3 | 4 | 5 | 5 | 6 | ||
---|---|---|---|---|---|---|---|---|---|
Mean | Music (n = 50) | off | — | 10.87 | 6.85 | 10.25 | 10.52 | 11.80 | 15.47 |
1 | 7.46 | 10.79 | 7.19 | 10.23 | 10.13 | 10.41 | 14.41 | ||
2 | 7.18 | 10.51 | 7.32 | 10.28 | 11.07 | 10.60 | 13.85 | ||
3 | 8.07 | 9.92 | 7.25 | 9.89 | 10.72 | 10.63 | 13.76 | ||
4 | 6.74 | 10.63 | 7.44 | 9.84 | 10.17 | 10.01 | 13.12 | ||
5 | 5.71 | 9.19 | 7.26 | 9.60 | 9.92 | 9.77 | 13.09 | ||
6 | 12.91 | 11.16 | 8.43 | 11.24 | 11.22 | 11.61 | 15.23 | ||
Speech (n = 38) | off | — | 9.96 | 5.91 | 9.56 | 10.33 | 10.19 | 12.45 | |
1 | 7.10 | 8.88 | 6.11 | 8.82 | 8.87 | 9.37 | 11.52 | ||
2 | 8.07 | 8.53 | 6.46 | 8.98 | 9.04 | 9.09 | 11.39 | ||
3 | 6.67 | 8.62 | 6.00 | 8.69 | 8.72 | 8.67 | 11.26 | ||
4 | 5.90 | 8.28 | 5.67 | 8.31 | 8.38 | 8.70 | 10.68 | ||
5 | 5.30 | 7.67 | 6.03 | 8.12 | 8.30 | 8.03 | 10.62 | ||
6 | 11.07 | 9.72 | 7.07 | 9.72 | 9.92 | 9.94 | 12.23 | ||
STD | Music (n = 50) | off | — | 4.36 | 3.04 | 3.53 | 2.59 | 3.54 | 3.61 |
1 | 3.54 | 3.77 | 2.40 | 3.03 | 3.07 | 3.10 | 3.83 | ||
2 | 4.03 | 3.78 | 2.74 | 3.11 | 3.01 | 2.94 | 3.63 | ||
3 | 4.19 | 3.15 | 2.90 | 2.83 | 3.20 | 3.05 | 3.69 | ||
4 | 3.09 | 4.24 | 2.69 | 2.77 | 3.03 | 2.88 | 4.42 | ||
5 | 2.93 | 3.08 | 2.73 | 3.15 | 3.39 | 3.30 | 4.04 | ||
6 | 4.83 | 4.12 | 3.90 | 3.57 | 3.58 | 4.17 | 3.72 | ||
Speech (n = 38) | off | — | 4.10 | 2.02 | 2.76 | 4.25 | 3.90 | 4.10 | |
1 | 3.52 | 3.15 | 2.28 | 2.74 | 2.67 | 2.95 | 4.44 | ||
2 | 4.17 | 3.01 | 2.55 | 2.81 | 2.50 | 3.02 | 3.95 | ||
3 | 3.04 | 3.46 | 2.12 | 2.73 | 2.36 | 2.44 | 4.48 | ||
4 | 2.75 | 2.81 | 1.93 | 2.63 | 2.57 | 3.08 | 4.54 | ||
5 | 3.34 | 3.21 | 2.55 | 2.38 | 2.91 | 2.67 | 4.83 | ||
6 | 5.82 | 3.70 | 2.73 | 3.01 | 3.37 | 3.60 | 4.03 |
Descriptive statistics of
Measure | off | 1 | 3 | 4 | 5 | 5 | 6 | ||
---|---|---|---|---|---|---|---|---|---|
Mean | Music (n = 50) | off | — | 30.76 | 24.39 | 18.36 | 25.03 | 18.79 | 32.28 |
1 | 16.89 | 29.38 | 22.66 | 19.48 | 22.94 | 19.32 | 30.06 | ||
2 | 18.36 | 28.97 | 24.10 | 19.05 | 23.75 | 19.34 | 30.63 | ||
3 | 14.99 | 29.95 | 22.94 | 18.96 | 22.22 | 18.97 | 29.94 | ||
4 | 12.51 | 28.70 | 22.78 | 18.20 | 22.07 | 18.88 | 28.26 | ||
5 | 10.56 | 27.02 | 22.66 | 17.99 | 22.45 | 18.11 | 28.13 | ||
6 | 31.88 | 31.52 | 26.18 | 21.73 | 25.64 | 23.12 | 33.40 | ||
Speech (n = 38) | off | — | 29.29 | 21.56 | 17.18 | 21.41 | 17.43 | 32.04 | |
1 | 16.19 | 26.51 | 22.53 | 17.71 | 20.37 | 18.69 | 29.28 | ||
2 | 20.75 | 27.78 | 23.43 | 19.07 | 20.69 | 19.34 | 30.03 | ||
3 | 11.53 | 25.70 | 21.71 | 17.15 | 19.36 | 17.39 | 28.98 | ||
4 | 9.85 | 25.07 | 20.89 | 16.29 | 19.54 | 17.00 | 26.69 | ||
5 | 8.42 | 24.60 | 20.91 | 17.04 | 18.94 | 16.96 | 26.69 | ||
6 | 31.93 | 29.47 | 26.04 | 20.85 | 23.25 | 21.30 | 33.11 | ||
STD | Music (n = 50) | off | — | 7.01 | 7.09 | 6.67 | 7.45 | 6.79 | 8.76 |
1 | 6.58 | 8.39 | 6.21 | 5.62 | 7.40 | 7.02 | 9.12 | ||
2 | 7.05 | 8.89 | 7.96 | 6.50 | 7.10 | 6.66 | 8.73 | ||
3 | 6.30 | 8.06 | 7.10 | 6.36 | 7.17 | 5.69 | 8.73 | ||
4 | 6.36 | 7.88 | 7.02 | 5.49 | 6.74 | 6.57 | 9.00 | ||
5 | 4.83 | 9.12 | 7.24 | 5.54 | 7.51 | 6.54 | 8.96 | ||
6 | 8.67 | 7.91 | 7.91 | 8.58 | 8.21 | 8.68 | 9.20 | ||
Speech (n = 38) | off | — | 9.68 | 7.31 | 5.97 | 9.02 | 7.43 | 9.43 | |
1 | 7.28 | 10.02 | 7.79 | 8.06 | 8.25 | 8.15 | 11.07 | ||
2 | 7.56 | 9.79 | 8.60 | 7.53 | 8.09 | 8.02 | 11.42 | ||
3 | 5.94 | 11.32 | 10.34 | 7.93 | 7.68 | 7.59 | 12.80 | ||
4 | 3.48 | 11.35 | 9.37 | 6.92 | 8.40 | 7.91 | 12.47 | ||
5 | 5.89 | 10.99 | 10.12 | 8.07 | 9.18 | 8.03 | 12.94 | ||
6 | 8.39 | 9.36 | 8.82 | 10.39 | 9.82 | 9.66 | 10.71 |
Descriptive statistics of
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\n\nWe have adopted the Protocol to increase the number of readers of our publications. All our Works are more widely accessible, with resulting benefits for scholars, researchers, students, libraries, universities and other academic institutions. Through this method of exposing metadata, IntechOpen enables citation indexes, scientific search engines, scholarly databases, and scientific literature collections to gather metadata from our repository and make our publications available to a broader academic audience.
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From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. 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Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. 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Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. 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