Carbon dots sizes and synthesis techniques.
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These books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\\n\\nThis collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\\n\\nTo celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
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IntechOpen and Knowledge Unlatched formed a partnership to support researchers working in engineering sciences by enabling an easier approach to publishing Open Access content. Using the Knowledge Unlatched crowdfunding model to raise the publishing costs through libraries around the world, Open Access Publishing Fee (OAPF) was not required from the authors.
\n\nInitially, the partnership supported engineering research, but it soon grew to include physical and life sciences, attracting more researchers to the advantages of Open Access publishing.
\n\n\n\nThese books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\n\nThis collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\n\nTo celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
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CDs can be classified as carbon nanomaterials that are less than 10 nm in size, they are the latest class of fluorescent nanoparticles [2]. CDs have attracted the interest of researchers in diverse fields of science and technology such as; optoelectronics [3], environmental pollution and remediation [4], biosensor [5], bio-imaging and biomedical applications [6, 7].
CDs possess properties such as being dimensionless, durable, large surface area, enhanced porosity and stability, ease of being functionalized, fluorescence emission, biocompatibility and low toxicity [5, 8]. These properties of CDs can be applied to improve the environment and human health [4, 9, 10].
A toxic rival to the CDs is the popular semiconductor nanocrystals popularly known as quantum dots (QDs). The QDs are a type of semiconductor nanoparticles with diameter range from 1 to 10 nm [11]. More so, QDs normally are made from semiconducting materials, especially iron and cadmium, which are highly toxic and expensive to acquire [12]. Compared to QDs, CDs are considered best option with a high degree of biocompatibility, cost-effectiveness and non-toxic. It also serves as a suitable substitute to QDs in numerous areas of research such as bio-imaging, bio-sensing, pharmaceutical and fuel cells [13, 14].
Carbon dots (CDs) are suitable for the modification of electrode sensors. It combines fundamental aspects of biology, chemistry, and physical sciences, computer science and electrical engineering to meet various needs in a wide application field. Therefore, carbon as a sensor portrays several meanings, conditional upon what field the user subscribes [15, 16].
Over the years, various bulk materials and several processes and techniques have been developed and adopted by a wide range of researchers in the synthesis of CDs. These processes include the hydrothermal and microwave-assisted routes, heating, biogenic synthesis, thermal oxidation, ultra-sonification, subcritical water process (use of oil bath and salt bath), refluxing and chemical oxidation [17, 18, 19, 20, 21, 22, 23, 24, 25, 26].
Three important factors must be considered in synthesizing CDs which are control of size, uniformity of CDs in solvents, and mitigated aggregation [27]. Wang and Hu [28] confirmed that CDs carbonaceous aggregation tends to form during carbonization but this can be prevented when synthesized by methods such as electrochemical synthesis, hydrothermal, or by pyrolysis method.
The application of biological and agro-waste to synthesize CDs have been advocated in numerous research such as; cooking oil waste [29], egg-white and egg-yolk [30], orange juice [6] as well as eggshells [31]. Though it is advantageous to use waste biomaterials in the synthesis of CDs to avoid competition with essential food production [32], however, the downside of the application of biomass in the synthesis of CDs is lacking of essential purity and structural homogeneity to obtain homogenous fluorescent CDs for purposes of sensing minute concentrations of analytes [21, 33]. These had caused the application of clean materials to be used in the synthesis of homogeneous fluorescent CDs [2].
A competent carbon source for soluble CDs synthesis is needed to comply with the goals of green chemistry and not be in direct competition with essential food production and should be cheap to synthesize [34, 35, 36]. Research in the synthesis of CDs must consider low price of additives and less purification steps in case of using biomass as a precursor material.
Thus, the emphasis is necessary on the cost of producing typical CDs, not to be a replica of the currently observed situation with semiconductor QDs, with the high cost and potential environmental negative impact and yet to achieve its full potential in commercial applications [37, 38, 39].
Carbon dots (CDs) have emerged to be attractive materials due to their excellent photoluminescence (PL) properties and wide surface areas, which are needed for sensitive and selective sensing of analytes [40]. These qualities are owed to the characteristics of the carbon element at nano-dimension and five valence electrons to bind carbon atoms [31, 41]. The green and sustainable carbons dots refer to CDs that are synthesized from agro and biomaterials that can be readily available without depleting their sources [42].
CDs can be obtained from various source [3]. These sources include plants and animal origins such as bamboo leaves, woods, green algae, sugar cane, mangosteen, carica papaya, saffron, gringko, neem gum, prawn shells, orange, cucumber and pineapple [32, 43, 44, 45]. Further interesting applications of CDs have been reported in diverse sectors of the environment and health fields of science and technology [3, 32].
For instance, Pattanayak and Nayak, in 2013 [43] presented an eco-friendly synthesis of iron nanoparticles from various plants and spices extract. The synthesis of nanoparticles from plant parts (leaf) is essential since this will not require expensive processes that are involved mostly in biomaterial processing. Iravani et al., [46] demonstrated a green synthesis of metal nanoparticle using plants (
Liu [44], reported a research work on one-step green synthesized fluorescent carbon nano-dots from bamboo leaves for copper (ll) ion detection and demonstrated the exploration of bamboo leaves as a carbon source. Carbon nano-dots were synthesized hydrothermally and a resultant high quantum yield quantum dots, with sensitive Cu2+ detection at a limit of detection as low as 115 nM on a dynamic range from 0.333 to 66.6 μM. The zeta potential of the pristine carbon quantum dots was measured at −4.78 mV which changes to +13.8 mV after treatment with positively charged polyethyleneimine (a water-soluble cationic polymer).
Wembo et al
Piyushi et al., [45] cultivated
Till et al., [48] synthesized CDs by microwave-assisted hydrothermal treatment of starch and Tris-acetate-EDTA. The process confirmed that nitrogen-doped CDs have emerged to be complementary to starch-derived CDs. Addition of nitrogen to CDs improved the yield of photoluminescence from 19% to 28%, making them promising luminescent materials for improving fluorescence of CDs. However, there is no added value in incurring additional chemicals during synthesis process of CDs. Starch is a better alternative to the use of nitrogen for synthesizing CDs. Till and colleagues observed the effect of nitrogen (N) additives, through the use of ethylenediaminetetraacetic acid (EDTA); tris (hydroxymethyl) aminomethane (Tris) and a combination of both (TAE-buffer) on the photophysical properties of CDs. Temperature (45 min at 230°C) plays an important role in the improved nitrogen-doped carbon structures [48].
Some researchers have adopted nitrogen for fluorescence and photoluminescence enhancement, but this approach has shown indistinct composition which required extensive purification steps. This, however, is environmentally not suitable and contravenes the concept of green chemistry since it involves many chemicals in the synthesis process [49].
Synthesis methods of CDs can be divided into two major parts; top-down and bottom-up as in Figure 1. Top-down starts from cutting the carbon materials into carbon particles or cleavage of larger carbonaceous materials such as carbon nanotube by laser ablation, arc discharge, electrochemical and candle/natural gas burner soot, and recently the hydrothermal route.
Synthesis methods of carbon dots (CDs).
The bottom-up route involves the use of molecules as support for localizing the growth of CDs by blocking aggregation during high-temperature treatment. However, this study explores the top-down process of CDs synthesis. As earlier stated the top-down approach concentrates on precursor carbonization that include microwave-assisted method, chemical oxidation, heating, and hydrothermal process [50].
It is a process removal of material from solid or liquid by irradiating it with a laser beam [51, 52, 53, 54]. Material evaporates or sublimates when the laser flux is low and converted to plasma at high laser flux. Goncalves and colleagues [51] reported the synthesis of CDs from carbon targets immersed in deionized water by direct laser ablation (UV pulsed laser irradiation). CDs were optimized and synthesized after being functionalized with NH2– polyethylene-glycol (PEG200) and N-acetyl-l-cysteine (NAC). To produce particles in tens of nanometer range by laser ablation, the energy is controlled within the incidence area of the precursor [54, 55].
Yu et al., [53] demonstrated the possibilities of relying on irradiating a toluene sample with a non-focused pulsed laser that is very different from the high powered laser irradiation employed in conventional ablation. This process by Yu and colleagues revealed an induced transformation of toluene into graphene sheaths, which subsequently produced fluorescent CDs. These nanoparticles can simply be functionalized using more than one molecule and stayed stable in an aqueous solution. It can also be applied to optical fiber devices through immobilization due to its stability in a specific optical nano-analytical sensor [56]. However, the equipment to conduct laser ablation is quite expensive and it needs technically skilled personnel to operate.
CDs were first discovered through this method accidentally when the separation of single-walled carbon nanotubes (SWNTs) were made using gel electrophoresis from carbon soot by arc discharge method. Carbon is formed when direct current arc voltage is applied in an inert gas across two graphite electrodes. The biggest challenge of this method is that it generates impurities that are difficult to purify [1].
Electrochemistry is another top-down approach used in synthesizing CDs, this process is facile and the product yield is normally high [57]. CDs with a size of 6 to 8 nm and 2.8% to 52% can be obtained through exfoliation that utilizes graphite rods and Pt wire in ionic liquid or water solution.
The mechanism of the exfoliation was due to complex interplay of anodic oxidative cleavage of water and anionic intercalation from the ionic liquid using titanium cathode and spectrum pure graphite in the center of electrolyzer to yield pure blue fluorescent CDs without the urgency of complex purification [28, 58, 59, 60, 61, 62].
Application of carbon soot in the synthesis of CDs have been reported by Tian et al., [63], the carbon source was from a carbon-processing reaction. Due to the simplicity to obtain the starting material, this method has been used widely by researchers. It also provides a new use for a complicated by-product. At the same time, it possesses disadvantages such as uncontrolled chemical surface, production of many byproducts that can harm human health with a broad dispersion [64].
Wang et al., [13] prepared CDs by microwave method. It proved reaction time can be shortened to 30–45 minutes with microwave-assisted technique. Similarly, Choi et al., [10] made effective use of lysine as a precursor to synthesize CDs within 5 minutes in a home type of microwave oven and the CDs were soluble in water with deep blue photoluminescence at a high mass yield of 23.3%.
Compared with other methods, the microwave route is more convenient since the heating of the carbon precursor is rapidly achieved within few minutes. It also exhibits high quantum yield and provides a long fluorescence lifetime. The procedure of microwave synthesis is much easier compared to others as it only utilizes heating via irradiation technique [65].
This method is mostly applied to produce CDs on an industrial scale. CDs can be obtained through oxidation treatment of carbon precursors by a strong oxidant. CDs from natural products have been researched and developed, by the synthesis of large scale CDs from human hair, coffee, and biomass by adding it into concentrated sulfuric acid and then heating at different temperatures. The time range is from hours to days [66]. By varying the temperature of synthesizing CDs, the quality of CDs such as diameter and quantum yields can be controlled [67, 68, 69].
The hydrothermal route of synthesizing CDs is considered as environmental-friendly, low cost and involves few synthesis steps that are non-toxic [2, 70, 71, 72, 73, 74]. Musa et al explored the hydrothermal method at a temperature range between 75°C to 175°C where the researchers reacted the precursor in a sealed hydrothermal reactor that resulted into a high yield photoluminescent quantum yield at 34.9% [2].
As illustrated in Figure 2, tapioca was added to an aldehyde solvent (acetone + sodium hydroxide) to improve the mobility of glucose molecules in starch [2]. The mixture underwent stages of reactions such as hydrolysis, adsorption, and gelatinization to particle disintegration simultaneously [2]. The carbonization temperature breaks the bond between the starch, making it available for the reactive solvent which leads to hydrolysis to form disaccharide and gelatinized glucose. The disaccharides polymerized into polysaccharides and the gelatinized glucose yielded CDs for functional group characterizations [9].
Mechanism for synthesis of carbon dots [
Like all-natural products, starch undergoes seasonal changes and particularly the amylose/amylopectin ratio is influenced by plant species and area of plant cultivation, which could influence the CDs formation. Independently of seasonal changes and origin, a starch will provide CDs with highly reproducible photoluminescent properties [2].
Substances such as glucose, citric acid, banana juice, and protein are examples of many precursors used to prepare CDs by adopting the hydrothermal route of synthesis [75]. Success has been reported in the synthesis of CDs through one-step hydrothermal carbonization using chitosan applied directly as a bioimaging agent [76]. The hydrothermal method is promising in producing CDs and is suitable for industrial or large scale production [75]. However, it is notable in 2010 where Zhang et al. [77] first reported a one-pot hydrothermal method to synthesize CDs from ascorbic acid in the presence of ethanol as solvent. Quantum yield and average particle sizes of their synthesized CDs were 6.79% and ~2 nm, respectively [77].
Several methods of synthesizing CDs has been explored in this section, to prevent the use of expensive precursor and energetic systems like in laser ablation. The hydrothermal synthesis route is being recommended as foremost for the sake of ecological sustainability [25]. Chemical oxidation and exfoliations provide an inexpensive alternative although it employs large amounts of strong acid which is hazardous and undesirable [77].
The other methods of synthesizing CDs need multi-step experimental operations and some of them require post-treatments to improve their water solubility, stability, and luminescent. Besides, several other methods suffer from drawbacks such as they require complex process and high temperature, time-consuming, harsh synthetic materials, and are expensive. This causes their applicability to be limited [78]. Several research successes proved that hydrothermal route to be a green method for the synthesis of CDs since the procedure produce soluble fluorescence CDs at reduce time and cost [2, 46, 79].
CDs derived from organic sources are excellent for the researcher and environment. Because the adoption of such material presents the choice to eliminate the need for metallic quantum dots, and any doping requirements, either through the use of sulfur (S) or nitrogen (N) agents. The use of metallic quantum dots and possible inclusion of S and N in enhancing their functionality contravenes the purpose of sustainable applications of nanomaterials in the modern field of nanotechnology [48].
Table 1 is a list of different synthesis techniques that have been attractive to researchers in recent years. The table provides a list of interesting techniques such as hydrothermal, microwave assisted, biogenic synthesis, thermal oxidation, ultrasonication, refluxing and chemical oxidation with excellent particle sizes [80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91]. The hydrothermal synthesis of CDs proves to be efficient and effective since it provides relatively smaller sizes of the nanoparticles as synthesized by Du et al., [81] at 1.8 nm, when compared to other methods such as chemical oxidation by Thambiraj and Shankaran [85] at 4.1 nm, biogenic synthesis by Phadke et al., [20] at 5–8 nm, and refluxing by Himaja et al., [84] at ~50 nm.
Method | Size (nm) | Reference |
---|---|---|
Microwave-assisted | 2.7 | [7] |
Microwave | 5–10 | [10] |
Biogenic synthesis | 5.0–8.0 | [20] |
Thermal oxidation | 5.0–10 | [22] |
Heating | 3.0 | [78] |
Hydrothermal | 2.3 | [80] |
Hydrothermal | 1.8 | [81] |
Ultrasonication | 5.0 | [82] |
“Oil bath” | 2.59 | [83] |
Refluxing | ~50 | [84] |
Chemical-oxidation | 4.1 | [85] |
Chemical oxidation | 2.5 | [86] |
Carbon dots sizes and synthesis techniques.
Note: n/a = not available.
One of the CDs properties is that it shows strong optical absorption in the UV region (200–800 nm) with a tail extending to the visible range, see Figure 3. CDs possess low toxicity with excellent photostability as compared to semi-conductor quantum dots [7, 23, 50, 71].
Optical properties of carbon dots at UV-visible absorption and emission spectra.
Absorption shoulders in the spectrum are due to the π-π* (pi to pi star transition) of C〓C bonds or n-π* (n to pi star transition) of C〓O and other fringe functional elements present [69, 92].
The uniqueness of CDs is the availability of wide surface area for trace detection of analytes and provision of adsorptive sites through the availability of heteroatomic carbon in nano-dimension along with photoluminescence emission. Based on past study, CDs is dependent on intensity and wavelength emission towards its excitation wavelength [93]. This is due to the different sizes of particles and surface chemistry and/or different emissive traps on CDs’ surface. The wavelength dependence behaviour makes CDs possible to be applied in multi-colour imaging and adsorptive purposes. Vinci et al
Table 2 shows the excitation wavelengths of CDs through the UV-lamp excitation process to obtain fluorescent characteristics [2].
Colour | Interval of wavelength (nm) |
---|---|
Red | 700–635 |
Orange | 635–590 |
Yellow | 590–560 |
Green | 560–520 |
Cyan | 520–490 |
Blue | 490–450 |
Violet | 450–400 |
The color range of visible light spectrum.
The colour of CDs most of the time is related to the surface groups which corresponds to particle sizes [93]. Normally CDs show strong photoluminescence from blue to green wavelength. To enhance the quantum yield (QY) of CDs or change photoluminescence (PL) emission to meet desired applications, surface passivation and functionality play a vital role. Besides, CDs show great photostability as there are no reductions in PL intensity with continuous exposure to excitation. In terms of chemical properties, different synthesis methods of CDs lead to different chemical structure and abundance of surface sites. They are usually connected or modified by polymer chains, oxygen-based, amino based groups, and others [93].
Characterization of CDs by high resolution transmission electron microscopy (HRTEM), Xray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR), Atomic force microscopy (AFM) and Zeta Potential provide deep insights into the attributes of CDs such as hybridization and coefficient between functional groups and carbon core that take parts in the provision of the abundance of surface sites and the photoluminescence behaviour [94]. In comparison to graphene and metallic quantum dots, the CDs serves as the way out of toxicity concerns in environmental monitoring and medical applications [95].
The sizes and texture of CDs are important for fundamental applications in the field of environmental science and nanotechnology. Figure 4(A–C), shows the HRTEM images of CDs at different resolutions between 1 nm to 10 nm. Synthesized CDs revealed amorphous quasi-spherical morphology with a lattice spacing of ca 0.24 nm (Figure 4A), CDs characteristics are suitable absorbent of pollutants that are larger than 0.24 nm [2, 71, 89].
High resolution transmission electron microscopic (HRTEM) (A) Lattice space of carbon dots (CDs) characterized in magnifications of 5 nm. (B) Images of CDs at 10 nm. (C) Size distribution of CDs within 10 nm magnification [
High-Resolution Transmission Electron Microscopic images of the CDs characterized in magnifications of 5 nm and 10 nm (Figure 4A and B respectively). Figure 4A is the lattice spacing for carbon dots at 5 nm magnification. While Figure 4B is the size distribution within 10 nm magnification. Figure 4C is the histogram chart, demonstrating the nanoparticle sizes of CDs. The synthesis of nanoparticle with low lattice space is needed for research applications of CDs in environmental chemistry, pollutant entrapment in aqueous media and water purification [74]. The interplanar distance (lattice spacing) of 0.24 nm (Figure 4) is lower than the lattice spacing planes of graphitic materials (0.34 nm), the larger interlayer spacing could be attributed to the abundant oxygen-containing groups. In other words, the oxygen-containing groups could expand the layer spacing. The synthesized CDs is in consonance with recent reports by Arumugam and colleagues, CDs was hydrothermally synthesized from broccoli [79], ginkgo fruits [87], and cabbage [8].
Fourier-transform Infrared spectroscopy (FTIR) portrays the functional structure of CDs. It reveals the intrinsic functional groups and other useful compounds present in CDs. Figure 5 provides functional groups that exist before and after the hydrothermal treatment of tapioca as a precursor for CDs.
FT-IR spectrum of the carbon dots and tapioca [
As shown in Figure 5(A) representing tapioca. Peaks associated with the stretching vibrations of hydroxyl (▬OH) and carboxylic (COO▬) groups are at 3353.45 and 2933.78 cm−1 [75]. Further stretching vibration of C▬H occurred from 1645.24 to 1341.82. The peaks at 1151.38, 1079.20, 1014.41 cm−1 can be due to the C▬O stretching vibrations and out-of-plane bending modes of sp2 and sp3 ▬CH group [75].
There were substantial changes observed in the spectra of CDs (Figure 5B). The hydroxyl (▬OH) group of 3389.71 cm−1 increased on the carbon structure as a result of hydrolysis. While the carboxylic (COO▬) group 2145.73 cm−1 reduced by thermal destruction of saccharides structure [34]. The peaks at 1695.27 cm−1 and 1644.62 cm−1 showed the increase in the C▬H stretching vibrations of the bending modes of the sp2 and sp3 ▬CH group. The peaks around 1427.63 cm−1 until 1369.43 cm−1 are due to C▬O▬C [34]. The peak at 1237.62 cm−1 corresponds to the C〓C stretching vibration while 1094.19 cm−1 and 996.19 cm−1 represents the C〓O stretching vibration and the last group at 706.78 cm−1 denotes the C〓C bond of the unsaturated glucose structure in the starch. These attributes were responsible for the water-soluble nature of CDs [34]. The FTIR graph shows the formation of unsaturated carbon. Along with oxygen-rich groups such as hydroxyl, carboxyl, and carbonyl situated on the CDs surface, which agree with the hydrothermal synthesized CDs from the organic origin [23, 25, 26, 81, 90].
There are numerous applications of carbon nanoparticles due to the abundant properties they possess [3]. These applications are being discussed in the subsequent sections of the report.
Carbon dots have shown great potential to act as a sensor and can be used for environmental monitoring and control of pollutants, more so in the medical field for biosensor applications. It can donate or accept electrons that make it suitable for detection of ions, vitamins, nucleic acid, protein, enzyme and biological pH value [7, 11, 96, 97, 98]. Even though different materials are used to detect specific ions, the detection mechanisms are identical [99].
The functional groups on the surface of CDs specify distinctive affinities to different target ions, through an electron or energy transfer process and high selectivity to other ions [100]. CDs has been involved in the detection of 2,4,6-tri-trotoluene (TNT) and also applied as a dual-sensing platform for fluorescent and electrochemical detection of TNT [101]. Other reports utilized CDs as pH sensors for in-vitro and in-vivo investigations [102].
Research showed CDs able to detect intracellular pH inside a living pathogenic fungal cell and has been developed to sense nucleic acid in the DNA [103]. In other cases, CDs have been used in bioimaging because of their low toxicity and excellent photostability compared to semi-conductor quantum dots that posed health problems and environmental concerns [8]. Its visible excitation, emission wavelengths, and high brightness confirm CDs as a suitable candidate in this area. Several studies have been conducted using CDs in cell imaging, including pig kidney cell line [104],
Graphical description of fluorescence images of carbon dots.
Chengkun et al., [98] discovered photoluminescence in CDs synthesized from Nescafe original instant coffee and applied it in the field of bioimaging. From their investigation, CDs from Nescafe are found to be amorphous and the cytotoxicity study revealed that the CDs did not cause any toxicity to human hepatocellular carcinoma cells at a concentration as high as 20 mg/ml. Yang et al., [107] also worked on novel green synthesis of high-fluorescent CDs from honey for sensing and imaging. It was an innovative and green approach towards a CDs of high fluorescent quantum yield and excellent photostability, employed for HeLa cells imaging and coding. Rui-jun et al
The application of CDs in the selective detection of heavy metals have been reported in several scientific and experimental research [34, 40, 79]. However, there are gaps and lapses needing redress, such applications are predominantly in photoluminescent quenching of heavy metals. whereas, current section looks into reliable and robust CDs for applications in electrochemical sensing of multiple ranges of heavy metal ions.
The development of a convenient and sustainable technique for detecting and identifying human and environmentally toxic metal ions is of great interest. The following are reports concerning CDs application in heavy metal detection.
Zhang and Chen [109] worked on nitrogen-doped carbon quantum dots application as a turn-off fluorescent probe for the detection of Hg2+ ions at a detection limit of 0.23 μM. The fluorescent quenching mechanism is attributed to the surface-state triggered by the mercury-induced conversion of special functional group (▬CONH▬) from spirolactam structure to an opened-ring amide [109].
Sandhya et al., [110] applied nanostructures for heavy metal ion sensing in water using surface plasmon resonance of metallic nanostructures. They reviewed on techniques to improve selectivity and sensitivity of surface plasmon response sensors with attention to homogeneity. Effects of particle size, shape, material type, and surrounding environment were found to be effectual in the surface plasmon surface frequency.
Similarly, Qu et al., [111] developed CDs to detect Fe3+ ions by using dopamine as a starting material with a detection limit of 0.32 μM. Quenching of photoluminescence intensity occurred when there was an interaction between CDs and ions. Meanwhile, Liu [44] reported a research work on one-step green synthesized fluorescent carbon nanodots from bamboo leaves for copper (ll) ion detection and demonstrated the exploration of bamboo leaves as a carbon source with high carbon constituent. Carbon quantum dots were synthesized hydrothermally with sensitive Cu2+ detection at limit of detection as low as 115 nM and a dynamic range from 0.333 to 66.6 μM. The zeta potential of the pristine carbon quantum dots was measured at −4.78 mV which improved to +13.8 mV after treatment with positively charged polyethyleneimine (a water-soluble cationic polymer). More so, Rao et al
Methionine has been used as a material for the synthesis of CDs [113]. These CDs were co-doped with nitrogen and sulfur to enhance surface functionalization for the detection and environmental monitoring of heavy metal pollutants [113]. Similarly, Shen et al
A fluorescent probe for selective detection of metal ions such as mercury (Hg2+, 1.00 × 10−8 − 1.50 × 10−3 M, 1.00 × 10−7 M) with wide linear range and satisfactory detection limits was discovered when citric acid monohydrate was used for the synthesis of fluorescent CDs [114]. More essentially and effective is the burning of ash from waste paper and further utilized as a source of CDs by Lin et al., [115]. They succeeded in synthesizing CDs without any surface modification and subsequently, the fluorescent CDs were quenched by Fe3+.
Simpson et al., [21] synthesized carbon nanoparticle from glycerol and phosphoric acid mixed in a Berghof high-pressure reactor at 250°C for 4 hours. Afterward, glassy carbon electrodes were fabricated by drop-casting the carbon nanoparticles, and further applied for heavy metal (Cu2+ and Pb2+) detection by square wave anodic stripping voltammetry [21]. Heavy metals such as Na+, K+, Mg2+, Ca2+, Cr3+, Co2+, Ag+, Hg2+, Cd2+, Pb2+, Ni2+, Cu2+, Zn2+, Al3+, Fe2+, and Fe3+ have been tested on CDs synthesized from carbon source of mangosteen pulp and a ground discovery was made. Among the listed heavy metals, Fe3+ was the favourite in detection with a detection limit of 52 nM. Further application was found for cell imaging, which reveals their diverse potential applications [89].
Abhishek et al., [14] made a paper strip based live cell ultrasensitive lead sensor using CDs synthesized from biological media. They reported a formulation of a sensor through microwave heating of potato-dextrose agar (PDA) for the detection of lead (pb2+) in solution but again involved a long and laborious process.
Pajewska et al. [116] explored the fluorescence of synthesized CDs from citric acid with glutathione for the sensing of mercury (Hg2+) ion. A high recovery of Hg2+ was achieved at 115.1%. The method of synthesizing CDs with low toxicity is embedded in the green chemistry principles. Thus, it fulfills the criteria of being eco-friendly. Table 2 provides a list of applications of CDs in the detection of heavy metals ions.
As seen in Table 3, the mechanism of action for the application of CDs largely depends on the analyte of concern. In the case of CDs from citric acid monohydrate for application in fluorescence quenching of Hg2+, it relies on Förster resonance energy transfer (FRET) [114]. This is similar to CDs synthesized from biomass [117], polyacrylamide [118], lotus root [119], degreased cotton [120], gold nanoclusters [111], and Petroleum coke [127].
Source of carbon nanoparticles | Sensing mechanism | Type of metal ions and linear range | Sensing (LOD) | Reference |
---|---|---|---|---|
Biomass | Fluorescence | Hg2+/Fe3+ 0.002 mol L−1 | 10.3 and 60.9 nM | [117] |
Polyacrylamide | Fluorescence | Hg2+ 0.25–50 μM | 13.48 nM | [118] |
Lotus plant | Fluorescence | Hg2+ 0.1 to 60.0 μM | 18.7 nM | [119] |
Degrease cotton | Fluorescence | Cr(VI) 1.00–6.00 mmol/L | 0.12 μg/mL | [120] |
Gold nanoparticles | Luminescence | Pb2+ 1 × 10−5 M | n/a | [121] |
Biomass from peanut shells | Fluorescence | Cu+2 0–5 mM | 4.8 mM | [122] |
Metal oxides | Electrochemical oxidation. | Cu2+ 0.1 to 1.3 μM | 0.04 μM | [123] |
Metal nitrates | Isotherm | Cd2+ 10 mg/L | 12.60 mg/g | [124] |
Mushroom | Fluorescent | Hg2+ 0 to 100 nM | 4.13 nM | [125] |
Penaeus merguiensis enzyme | Isotherm | Cu+2 1–5 mM | 2 mM | [126] |
Coke | Fluorescent | Cu+2 0.25–10 μM | 0.0295 μM | [127] |
Carbon nanoparticles for heavy metal sensing.
Fluorescent carbon nanoparticle sensing is largely dependent on changes or disturbances that are caused by an analyte that interacts with a fluorescent probe. This shift mostly will lead to a measurable change in the emission characteristics of the probe (emission wavelength, intensity, lifetime, or anisotropy), which can be directly linked to analytes (e.g heavy metal) concentration. More so, fluorescence probe strategies are based on quenching (turn-off) or enhancing (turn-on) emission, and surface-enhanced Raman scattering (SERS) techniques [33, 125].
Other notable techniques for the detection and quantification of heavy metals ions include, inductively coupled plasma mass spectrometry (ICP-MS). This instrumentation is efficient among several other methods, but it is expensive. It was developed since the 1980s [128, 129, 130], used mostly by multivariate analysis along with the ICP-MS technique to unravel heavy metal elements present samples. However, inductively coupled plasma atomic emission spectroscopy (ICP-AES) have also been used to identify heavy metal pollutants. But, the method is expensive and requires sophisticated instrumentations and a highly trained technician [131].
Nowadays, marine pollution is becoming a global phenomenon and seafood safety has played a crucial role in human health [129]. Fatema et al., [132] applied atomic absorption spectroscopy (AAS) to measure the absorbed quantity of Pb+2, Cd+2, and Hg+3 in shrimps. Heavy metals have been detected by other means such as energy dispersive x-ray fluorescence (EDXRF), electrothermal atomic absorption method (ETAAS), and flame atomic absorption spectroscopy (FAAS) [133, 134]. But, all of the aforementioned techniques have disadvantages in the detection of heavy metals, such that they are expensive and require strenuous experimental steps [135]. Therefore, environmental researchers have continued to strive to develop a cheap, simple, sensitive, specific, accurate, user-friendly, and eco-friendly means of detection for heavy metal pollutants.
CDs are very useful in detecting non-metallic elements. Several types of research have been reported, CDs synthesized from potato are well able to detect phosphate [106]. Zhaoxia et al., [136] utilized CDs with turnable emission and controlled size for sensing hypochlorous acid. As a class of carbohydrate that is widely distributed in a living organism, sucrose was chosen as a carbon source with assistance of microwave irradiation. A strongly fluorescent CDs without post-passivation was produced. By increasing the concentration of phosphoric acid as fluorescence enhancer under UV lamp, various fluorescent emissions of CDs of variable sizes were obtained. It was found that green CDs have excellent sensitivity for the detection of hypochlorous acid.
Kuo et al
Huilin et al., [137], investigated on CDs as a fluorescent probe for off-on detection of sodium dodecyl-benzenesulfonate (SDBS) in aqueous solution. The pristine CDs were synthesized from sodium citrate through a simple, convenient, and one-step hydrothermal method. Fluorescent recovery was achieved with the application of SDBS. Detection of SDBS in real water samples was proportional to the concentration in the range of 0.10 to 7.50 ug/mL. Furthermore, fluorescence sensing probe has been used to detect kaempferol (flavonoid that is present in a variety of plants and plant-derived foods) using fluorescent CDs synthesized from chiefly acetic acid with a detection limit of 38.4 nM in the concentration range of 3.5–49 μM. Finally, organophosphorus as pesticides have been detected through the use of CDs as a detector for pollutants without surface modification [115].
CDs and carbon structured nanoparticles have attracted researchers to explore their effectiveness and optimization ability in the fields of pollution research [3]. Because of their excellent properties; carbon material performs concurrently as adsorbent and a transducing-agent [138, 139, 140, 141].
Due to abundant surface sites provided by CDs, it is a suitable candidate for studies in the detection and adsorption of heavy metals [142, 143]. For instance, Ghiloufi et al., [144] used gallium doped zinc oxide (ZnO) nanoparticle in the adsorption of heavy metals (Cd2+ and Cr6+) in aqueous solution. The adsorption of heavy metals was analyzed through the effect of pH and it revealed favourable adsorption at a low pH level, less than pH-3 and temperature of 298 K [144].
Table 4 provides harmonized presentation of nanomaterials applied for the purpose of absorbing environmental pollutants and contaminants in aqueous systems [145, 146, 147, 148, 149, 150, 151, 152, 153, 154].
Adsorbent material | Adsorbate/analyte | Reference |
---|---|---|
Gold nanoparticles (AuNPs) | 4-nitrophenol | [145] |
Carbon dots (sodium citrate) | Mercury (II) ions. | [146] |
Fluorescent carbon dots from o- phenylenediamine | Cell imaging and sensitive detection of Fe3+ and H2O2 | [18] |
Silica gel | Aromatic volatile organic compounds (VOCs) | [147] |
Graphene oxide | Nitrobenzene in sulfide | [148] |
TiO2, SiO2, and ZnO nanoparticles | Neptunium (V) | [149] |
Polystyrene latex nanoparticles | Alumina | [150] |
Graphene oxide | Radionuclide removal | [151] |
Polyaniline modified graphene oxide | Uranium(VI) | [152] |
Carbon nanotubes | Mingle-ringed N- and S-heterocyclic aromatics | [153] |
Graphene oxide | Minerals such as montmorillonite, kaolinite, and goethite, in aqueous phase | [154] |
Nanostructured materials in adsorption processes.
So far the concept of applying nanoparticles for environmental objectives have been successful. Meanwhile it is recommended that comparisons be made with bulk counterparts of the same substance to measure efficiency. On this note a study on the application of bulk agro material from
A suitable carbon source for CDs synthesis should be soluble in water (green chemistry), accessible worldwide (i.e. geographical abundance) with defined and well-known properties (i.e. functional attributes), should not be in direct competition with essential food production (i.e. sustainable), and it should be cost-effective (i.e. cheaply accessible). While the price of additives or carbon source plays a minor role in fundamental research, it may play a major role when large quantities are considered.
The authors would like to thank Universiti Putra Malaysia (UPM), Malaysia for funding this article.
The authors hereby declare that there is no conflict of interest.
M.Y.P., as the first author; made the study conception and design acquisition of reports and drafting of manuscript. Z.Z.A., contributed in the study conception and design, critical revision of major scientific ideas through clinical experience.
This research was funded by Universiti Putra Malaysia, grant number GP-IPS/2017/9556800.
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He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:"Polytechnic University of Timişoara",institution:{name:"Polytechnic University of Timişoara",institutionURL:null,country:{name:"Romania"}}}]},{type:"book",id:"9963",title:"Advances and Applications in Deep Learning",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9963.jpg",slug:"advances-and-applications-in-deep-learning",publishedDate:"December 9th 2020",editedByType:"Edited by",bookSignature:"Marco Antonio Aceves-Fernandez",hash:"0d51ba46f22e55cb89140f60d86a071e",volumeInSeries:4,fullTitle:"Advances and Applications in Deep Learning",editors:[{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. 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His research interests include intelligent and embedded systems.",institutionString:"Universidad Autonoma de Queretaro",institution:{name:"Autonomous University of Queretaro",institutionURL:null,country:{name:"Mexico"}}}]}]},openForSubmissionBooks:{},onlineFirstChapters:{paginationCount:18,paginationItems:[{id:"81778",title:"Influence of Mechanical Properties of Biomaterials on the Reconstruction of Biomedical Parts via Additive Manufacturing Techniques: An Overview",doi:"10.5772/intechopen.104465",signatures:"Babatunde Olamide Omiyale, Akeem Abiodun Rasheed, Robinson Omoboyode Akinnusi and Temitope Olumide Olugbade",slug:"influence-of-mechanical-properties-of-biomaterials-on-the-reconstruction-of-biomedical-parts-via-add",totalDownloads:0,totalCrossrefCites:null,totalDimensionsCites:null,authors:null,book:{title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11405.jpg",subseries:{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering"}}},{id:"81751",title:"NanoBioSensors: From Electrochemical Sensors Improvement to Theranostic Applications",doi:"10.5772/intechopen.102552",signatures:"Anielle C.A. 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His research interest focuses on computational chemistry and molecular modeling of diverse systems of pharmacological, food, and alternative energy interests by resorting to DFT and Conceptual DFT. He has authored a coauthored more than 255 peer-reviewed papers, 32 book chapters, and 2 edited books. He has delivered speeches at many international and domestic conferences. He serves as a reviewer for more than eighty international journals, books, and research proposals as well as an editor for special issues of renowned scientific journals.",institutionString:"Centro de Investigación en Materiales Avanzados",institution:{name:"Centro de Investigación en Materiales Avanzados",country:{name:"Mexico"}}},{id:"76477",title:"Prof.",name:"Mirza",middleName:null,surname:"Hasanuzzaman",slug:"mirza-hasanuzzaman",fullName:"Mirza Hasanuzzaman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/76477/images/system/76477.png",biography:"Dr. Mirza Hasanuzzaman is a Professor of Agronomy at Sher-e-Bangla Agricultural University, Bangladesh. He received his Ph.D. in Plant Stress Physiology and Antioxidant Metabolism from Ehime University, Japan, with a scholarship from the Japanese Government (MEXT). Later, he completed his postdoctoral research at the Center of Molecular Biosciences, University of the Ryukyus, Japan, as a recipient of the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship. He was also the recipient of the Australian Government Endeavour Research Fellowship for postdoctoral research as an adjunct senior researcher at the University of Tasmania, Australia. Dr. Hasanuzzaman’s current work is focused on the physiological and molecular mechanisms of environmental stress tolerance. Dr. Hasanuzzaman has published more than 150 articles in peer-reviewed journals. He has edited ten books and written more than forty book chapters on important aspects of plant physiology, plant stress tolerance, and crop production. According to Scopus, Dr. Hasanuzzaman’s publications have received more than 10,500 citations with an h-index of 53. He has been named a Highly Cited Researcher by Clarivate. He is an editor and reviewer for more than fifty peer-reviewed international journals and was a recipient of the “Publons Peer Review Award” in 2017, 2018, and 2019. He has been honored by different authorities for his outstanding performance in various fields like research and education, and he has received the World Academy of Science Young Scientist Award (2014) and the University Grants Commission (UGC) Award 2018. He is a fellow of the Bangladesh Academy of Sciences (BAS) and the Royal Society of Biology.",institutionString:"Sher-e-Bangla Agricultural University",institution:{name:"Sher-e-Bangla Agricultural University",country:{name:"Bangladesh"}}},{id:"187859",title:"Prof.",name:"Kusal",middleName:"K.",surname:"Das",slug:"kusal-das",fullName:"Kusal Das",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSBDeQAO/Profile_Picture_1623411145568",biography:"Kusal K. Das is a Distinguished Chair Professor of Physiology, Shri B. M. Patil Medical College and Director, Centre for Advanced Medical Research (CAMR), BLDE (Deemed to be University), Vijayapur, Karnataka, India. Dr. Das did his M.S. and Ph.D. in Human Physiology from the University of Calcutta, Kolkata. His area of research is focused on understanding of molecular mechanisms of heavy metal activated low oxygen sensing pathways in vascular pathophysiology. He has invented a new method of estimation of serum vitamin E. His expertise in critical experimental protocols on vascular functions in experimental animals was well documented by his quality of publications. He was a Visiting Professor of Medicine at University of Leeds, United Kingdom (2014-2016) and Tulane University, New Orleans, USA (2017). For his immense contribution in medical research Ministry of Science and Technology, Government of India conferred him 'G.P. Chatterjee Memorial Research Prize-2019” and he is also the recipient of 'Dr.Raja Ramanna State Scientist Award 2015” by Government of Karnataka. He is a Fellow of the Royal Society of Biology (FRSB), London and Honorary Fellow of Karnataka Science and Technology Academy, Department of Science and Technology, Government of Karnataka.",institutionString:"BLDE (Deemed to be University), India",institution:null},{id:"243660",title:"Dr.",name:"Mallanagouda Shivanagouda",middleName:null,surname:"Biradar",slug:"mallanagouda-shivanagouda-biradar",fullName:"Mallanagouda Shivanagouda Biradar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243660/images/system/243660.jpeg",biography:"M. S. Biradar is Vice Chancellor and Professor of Medicine of\nBLDE (Deemed to be University), Vijayapura, Karnataka, India.\nHe obtained his MD with a gold medal in General Medicine and\nhas devoted himself to medical teaching, research, and administrations. He has also immensely contributed to medical research\non vascular medicine, which is reflected by his numerous publications including books and book chapters. Professor Biradar was\nalso Visiting Professor at Tulane University School of Medicine, New Orleans, USA.",institutionString:"BLDE (Deemed to be University)",institution:{name:"BLDE University",country:{name:"India"}}},{id:"289796",title:"Dr.",name:"Swastika",middleName:null,surname:"Das",slug:"swastika-das",fullName:"Swastika Das",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/289796/images/system/289796.jpeg",biography:"Swastika N. Das is Professor of Chemistry at the V. P. Dr. P. G.\nHalakatti College of Engineering and Technology, BLDE (Deemed\nto be University), Vijayapura, Karnataka, India. She obtained an\nMSc, MPhil, and PhD in Chemistry from Sambalpur University,\nOdisha, India. Her areas of research interest are medicinal chemistry, chemical kinetics, and free radical chemistry. She is a member\nof the investigators who invented a new modified method of estimation of serum vitamin E. She has authored numerous publications including book\nchapters and is a mentor of doctoral curriculum at her university.",institutionString:"BLDEA’s V.P.Dr.P.G.Halakatti College of Engineering & Technology",institution:{name:"BLDE University",country:{name:"India"}}},{id:"248459",title:"Dr.",name:"Akikazu",middleName:null,surname:"Takada",slug:"akikazu-takada",fullName:"Akikazu Takada",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248459/images/system/248459.png",biography:"Akikazu Takada was born in Japan, 1935. After graduation from\nKeio University School of Medicine and finishing his post-graduate studies, he worked at Roswell Park Memorial Institute NY,\nUSA. He then took a professorship at Hamamatsu University\nSchool of Medicine. In thrombosis studies, he found the SK\npotentiator that enhances plasminogen activation by streptokinase. He is very much interested in simultaneous measurements\nof fatty acids, amino acids, and tryptophan degradation products. By using fatty\nacid analyses, he indicated that plasma levels of trans-fatty acids of old men were\nfar higher in the US than Japanese men. . He also showed that eicosapentaenoic acid\n(EPA) and docosahexaenoic acid (DHA) levels are higher, and arachidonic acid\nlevels are lower in Japanese than US people. By using simultaneous LC/MS analyses\nof plasma levels of tryptophan metabolites, he recently found that plasma levels of\nserotonin, kynurenine, or 5-HIAA were higher in patients of mono- and bipolar\ndepression, which are significantly different from observations reported before. In\nview of recent reports that plasma tryptophan metabolites are mainly produced by\nmicrobiota. He is now working on the relationships between microbiota and depression or autism.",institutionString:"Hamamatsu University School of Medicine",institution:{name:"Hamamatsu University School of Medicine",country:{name:"Japan"}}},{id:"137240",title:"Prof.",name:"Mohammed",middleName:null,surname:"Khalid",slug:"mohammed-khalid",fullName:"Mohammed Khalid",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/137240/images/system/137240.png",biography:"Mohammed Khalid received his B.S. degree in chemistry in 2000 and Ph.D. degree in physical chemistry in 2007 from the University of Khartoum, Sudan. He moved to School of Chemistry, Faculty of Science, University of Sydney, Australia in 2009 and joined Dr. Ron Clarke as a postdoctoral fellow where he worked on the interaction of ATP with the phosphoenzyme of the Na+/K+-ATPase and dual mechanisms of allosteric acceleration of the Na+/K+-ATPase by ATP; then he went back to Department of Chemistry, University of Khartoum as an assistant professor, and in 2014 he was promoted as an associate professor. In 2011, he joined the staff of Department of Chemistry at Taif University, Saudi Arabia, where he is currently an assistant professor. His research interests include the following: P-Type ATPase enzyme kinetics and mechanisms, kinetics and mechanisms of redox reactions, autocatalytic reactions, computational enzyme kinetics, allosteric acceleration of P-type ATPases by ATP, exploring of allosteric sites of ATPases, and interaction of ATP with ATPases located in cell membranes.",institutionString:"Taif University",institution:{name:"Taif University",country:{name:"Saudi Arabia"}}},{id:"63810",title:"Prof.",name:"Jorge",middleName:null,surname:"Morales-Montor",slug:"jorge-morales-montor",fullName:"Jorge Morales-Montor",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/63810/images/system/63810.png",biography:"Dr. Jorge Morales-Montor was recognized with the Lola and Igo Flisser PUIS Award for best graduate thesis at the national level in the field of parasitology. He received a fellowship from the Fogarty Foundation to perform postdoctoral research stay at the University of Georgia. He has 153 journal articles to his credit. He has also edited several books and published more than fifty-five book chapters. He is a member of the Mexican Academy of Sciences, Latin American Academy of Sciences, and the National Academy of Medicine. He has received more than thirty-five awards and has supervised numerous bachelor’s, master’s, and Ph.D. students. Dr. Morales-Montor is the past president of the Mexican Society of Parasitology.",institutionString:"National Autonomous University of Mexico",institution:{name:"National Autonomous University of Mexico",country:{name:"Mexico"}}},{id:"217215",title:"Dr.",name:"Palash",middleName:null,surname:"Mandal",slug:"palash-mandal",fullName:"Palash Mandal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217215/images/system/217215.jpeg",biography:null,institutionString:"Charusat University",institution:null},{id:"49739",title:"Dr.",name:"Leszek",middleName:null,surname:"Szablewski",slug:"leszek-szablewski",fullName:"Leszek Szablewski",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49739/images/system/49739.jpg",biography:"Leszek Szablewski is a professor of medical sciences. He received his M.S. in the Faculty of Biology from the University of Warsaw and his PhD degree from the Institute of Experimental Biology Polish Academy of Sciences. He habilitated in the Medical University of Warsaw, and he obtained his degree of Professor from the President of Poland. Professor Szablewski is the Head of Chair and Department of General Biology and Parasitology, Medical University of Warsaw. Professor Szablewski has published over 80 peer-reviewed papers in journals such as Journal of Alzheimer’s Disease, Biochim. Biophys. Acta Reviews of Cancer, Biol. Chem., J. Biomed. Sci., and Diabetes/Metabol. Res. Rev, Endocrine. He is the author of two books and four book chapters. He has edited four books, written 15 scripts for students, is the ad hoc reviewer of over 30 peer-reviewed journals, and editorial member of peer-reviewed journals. Prof. Szablewski’s research focuses on cell physiology, genetics, and pathophysiology. He works on the damage caused by lack of glucose homeostasis and changes in the expression and/or function of glucose transporters due to various diseases. He has given lectures, seminars, and exercises for students at the Medical University.",institutionString:"Medical University of Warsaw",institution:{name:"Medical University of Warsaw",country:{name:"Poland"}}},{id:"173123",title:"Dr.",name:"Maitham",middleName:null,surname:"Khajah",slug:"maitham-khajah",fullName:"Maitham Khajah",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/173123/images/system/173123.jpeg",biography:"Dr. Maitham A. Khajah received his degree in Pharmacy from Faculty of Pharmacy, Kuwait University, in 2003 and obtained his PhD degree in December 2009 from the University of Calgary, Canada (Gastrointestinal Science and Immunology). Since January 2010 he has been assistant professor in Kuwait University, Faculty of Pharmacy, Department of Pharmacology and Therapeutics. His research interest are molecular targets for the treatment of inflammatory bowel disease (IBD) and the mechanisms responsible for immune cell chemotaxis. He cosupervised many students for the MSc Molecular Biology Program, College of Graduate Studies, Kuwait University. Ever since joining Kuwait University in 2010, he got various grants as PI and Co-I. He was awarded the Best Young Researcher Award by Kuwait University, Research Sector, for the Year 2013–2014. He was a member in the organizing committee for three conferences organized by Kuwait University, Faculty of Pharmacy, as cochair and a member in the scientific committee (the 3rd, 4th, and 5th Kuwait International Pharmacy Conference).",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"195136",title:"Dr.",name:"Aya",middleName:null,surname:"Adel",slug:"aya-adel",fullName:"Aya Adel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/195136/images/system/195136.jpg",biography:"Dr. Adel works as an Assistant Lecturer in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. Dr. Adel is especially interested in joint attention and its impairment in autism spectrum disorder",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"94911",title:"Dr.",name:"Boulenouar",middleName:null,surname:"Mesraoua",slug:"boulenouar-mesraoua",fullName:"Boulenouar Mesraoua",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94911/images/system/94911.png",biography:"Dr Boulenouar Mesraoua is the Associate Professor of Clinical Neurology at Weill Cornell Medical College-Qatar and a Consultant Neurologist at Hamad Medical Corporation at the Neuroscience Department; He graduated as a Medical Doctor from the University of Oran, Algeria; he then moved to Belgium, the City of Liege, for a Residency in Internal Medicine and Neurology at Liege University; after getting the Belgian Board of Neurology (with high marks), he went to the National Hospital for Nervous Diseases, Queen Square, London, United Kingdom for a fellowship in Clinical Neurophysiology, under Pr Willison ; Dr Mesraoua had also further training in Epilepsy and Continuous EEG Monitoring for two years (from 2001-2003) in the Neurophysiology department of Zurich University, Switzerland, under late Pr Hans Gregor Wieser ,an internationally known epileptologist expert. \n\nDr B. Mesraoua is the Director of the Neurology Fellowship Program at the Neurology Section and an active member of the newly created Comprehensive Epilepsy Program at Hamad General Hospital, Doha, Qatar; he is also Assistant Director of the Residency Program at the Qatar Medical School. \nDr B. Mesraoua's main interests are Epilepsy, Multiple Sclerosis, and Clinical Neurology; He is the Chairman and the Organizer of the well known Qatar Epilepsy Symposium, he is running yearly for the past 14 years and which is considered a landmark in the Gulf region; He has also started last year , together with other epileptologists from Qatar, the region and elsewhere, a yearly International Epilepsy School Course, which was attended by many neurologists from the Area.\n\nInternationally, Dr Mesraoua is an active and elected member of the Commission on Eastern Mediterranean Region (EMR ) , a regional branch of the International League Against Epilepsy (ILAE), where he represents the Middle East and North Africa(MENA ) and where he holds the position of chief of the Epilepsy Epidemiology Section; Dr Mesraoua is a member of the American Academy of Neurology, the Europeen Academy of Neurology and the American Epilepsy Society.\n\nDr Mesraoua's main objectives are to encourage frequent gathering of the epileptologists/neurologists from the MENA region and the rest of the world, promote Epilepsy Teaching in the MENA Region, and encourage multicenter studies involving neurologists and epileptologists in the MENA region, particularly epilepsy epidemiological studies. \n\nDr. Mesraoua is the recipient of two research Grants, as the Lead Principal Investigator (750.000 USD and 250.000 USD) from the Qatar National Research Fund (QNRF) and the Hamad Hospital Internal Research Grant (IRGC), on the following topics : “Continuous EEG Monitoring in the ICU “ and on “Alpha-lactoalbumin , proof of concept in the treatment of epilepsy” .Dr Mesraoua is a reviewer for the journal \"seizures\" (Europeen Epilepsy Journal ) as well as dove journals ; Dr Mesraoua is the author and co-author of many peer reviewed publications and four book chapters in the field of Epilepsy and Clinical Neurology",institutionString:"Weill Cornell Medical College in Qatar",institution:{name:"Weill Cornell Medical College in Qatar",country:{name:"Qatar"}}},{id:"282429",title:"Prof.",name:"Covanis",middleName:null,surname:"Athanasios",slug:"covanis-athanasios",fullName:"Covanis Athanasios",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/282429/images/system/282429.jpg",biography:null,institutionString:"Neurology-Neurophysiology Department of the Children Hospital Agia Sophia",institution:null},{id:"190980",title:"Prof.",name:"Marwa",middleName:null,surname:"Mahmoud Saleh",slug:"marwa-mahmoud-saleh",fullName:"Marwa Mahmoud Saleh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/190980/images/system/190980.jpg",biography:"Professor Marwa Mahmoud Saleh is a doctor of medicine and currently works in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. She got her doctoral degree in 1991 and her doctoral thesis was accomplished in the University of Iowa, United States. Her publications covered a multitude of topics as videokymography, cochlear implants, stuttering, and dysphagia. She has lectured Egyptian phonology for many years. Her recent research interest is joint attention in autism.",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"259190",title:"Dr.",name:"Syed Ali Raza",middleName:null,surname:"Naqvi",slug:"syed-ali-raza-naqvi",fullName:"Syed Ali Raza Naqvi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259190/images/system/259190.png",biography:"Dr. Naqvi is a radioanalytical chemist and is working as an associate professor of analytical chemistry in the Department of Chemistry, Government College University, Faisalabad, Pakistan. Advance separation techniques, nuclear analytical techniques and radiopharmaceutical analysis are the main courses that he is teaching to graduate and post-graduate students. In the research area, he is focusing on the development of organic- and biomolecule-based radiopharmaceuticals for diagnosis and therapy of infectious and cancerous diseases. Under the supervision of Dr. Naqvi, three students have completed their Ph.D. degrees and 41 students have completed their MS degrees. He has completed three research projects and is currently working on 2 projects entitled “Radiolabeling of fluoroquinolone derivatives for the diagnosis of deep-seated bacterial infections” and “Radiolabeled minigastrin peptides for diagnosis and therapy of NETs”. He has published about 100 research articles in international reputed journals and 7 book chapters. Pakistan Institute of Nuclear Science & Technology (PINSTECH) Islamabad, Punjab Institute of Nuclear Medicine (PINM), Faisalabad and Institute of Nuclear Medicine and Radiology (INOR) Abbottabad are the main collaborating institutes.",institutionString:"Government College University",institution:{name:"Government College University, Faisalabad",country:{name:"Pakistan"}}},{id:"58390",title:"Dr.",name:"Gyula",middleName:null,surname:"Mozsik",slug:"gyula-mozsik",fullName:"Gyula Mozsik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/58390/images/system/58390.png",biography:"Gyula Mózsik MD, Ph.D., ScD (med), is an emeritus professor of Medicine at the First Department of Medicine, Univesity of Pécs, Hungary. He was head of this department from 1993 to 2003. His specializations are medicine, gastroenterology, clinical pharmacology, clinical nutrition, and dietetics. His research fields are biochemical pharmacological examinations in the human gastrointestinal (GI) mucosa, mechanisms of retinoids, drugs, capsaicin-sensitive afferent nerves, and innovative pharmacological, pharmaceutical, and nutritional (dietary) research in humans. He has published about 360 peer-reviewed papers, 197 book chapters, 692 abstracts, 19 monographs, and has edited 37 books. He has given about 1120 regular and review lectures. He has organized thirty-eight national and international congresses and symposia. He is the founder of the International Conference on Ulcer Research (ICUR); International Union of Pharmacology, Gastrointestinal Section (IUPHAR-GI); Brain-Gut Society symposiums, and gastrointestinal cytoprotective symposiums. He received the Andre Robert Award from IUPHAR-GI in 2014. 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From 1985 to 2004, he served as a Full Professor of Biochemistry at the Universidad Nacional de La Plata. He is a member of the National Research Council (CONICET), Argentina, and the Argentine Society for Biochemistry and Molecular Biology (SAIB). His laboratory has been interested for many years in the lipid peroxidation of biological membranes from various tissues and different species. Dr. Catalá has directed twelve doctoral theses, published more than 100 papers in peer-reviewed journals, several chapters in books, and edited twelve books. He received awards at the 40th International Conference Biochemistry of Lipids 1999 in Dijon, France. He is the winner of the Bimbo Pan-American Nutrition, Food Science and Technology Award 2006 and 2012, South America, Human Nutrition, Professional Category. In 2006, he won the Bernardo Houssay award in pharmacology, in recognition of his meritorious works of research. 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At the National Cancer Institute (National Institute of Health, Bethesda, MD) he worked as a research associate on the molecular biology of selenium and its role in health and disease. After postdoctoral collaborations with Carlos Gutierrez-Merino (University of Extremadura, Spain) and Dario Alessi (University of Dundee, UK), he established his own laboratory in 2008. 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In many cases, these diseases have adapted so well that they have developed efficient resilience methods in the human host and can live in the host for years. Others, particularly some blood parasites, can cause very acute diseases and are responsible for millions of deaths yearly. Many parasitic diseases are classified as neglected tropical diseases because they have received minimal funding over recent years and, in many cases, are under-reported despite the critical role they play in morbidity and mortality among human and animal hosts. The current topic, Parasitic Infectious Diseases, in the Infectious Diseases Series aims to publish studies on the systematics, epidemiology, molecular biology, genomics, pathogenesis, genetics, and clinical significance of parasitic diseases from blood borne to intestinal parasites as well as zoonotic parasites. We hope to cover all aspects of parasitic diseases to provide current and relevant research data on these very important diseases. In the current atmosphere of the Coronavirus pandemic, communities around the world, particularly those in different underdeveloped areas, are faced with the growing challenges of the high burden of parasitic diseases. At the same time, they are faced with the Covid-19 pandemic leading to what some authors have called potential syndemics that might worsen the outcome of such infections. 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He is currently a rated researcher by the National Research Foundation of South Africa at category C2. He has published widely in the field of infectious diseases and has overseen several MSc’s and PhDs. His research activities mostly cover topics on infectious diseases from epidemiology to control. His particular interest lies in the study of intestinal protozoan parasites and opportunistic infections among HIV patients as well as the potential impact of childhood diarrhoea on growth and child development. He also conducts research on water-borne diseases and water quality and is involved in the evaluation of point-of-use water treatment technologies using silver and copper nanoparticles in collaboration with the University of Virginia, USA. 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