Open access peer-reviewed chapter

Screening of Soybean (Glycine Max (L.) Merrill) Genotypes for Resistance to Rust, Yellow Mosaic and Pod Shattering

By M. H. Khan, S. D. Tyagi and Z. A. Dar

Submitted: March 1st 2012Reviewed: October 26th 2012Published: February 13th 2013

DOI: 10.5772/54697

Downloaded: 1582

1. Introduction

Soybean (Glycine max (L.) Merrill) is known as ‘Golden bean’ and miracle crop of 20th century. Soybean is a native of North China, Asia belongs to family fabaceae. It is a versatile and fascinating crop with innumerable possibilities of not only improving agriculture but also supporting industries. Soybean besides having high yielding potential (40-45 q/ha) also provides cholesterol free oil (20%) and high quality protein (40%). It is a rich source of lysine (6.4%) in addition to other essential amino acids, vitamins and minerals. Its oil is also used as a raw material in manufacturing antibiotics, paints, varnishes, adhesives and lubricants etc.

Like other economically important crops soybean is also suffering from many diseases viz, rust (Phakopsora pachyrhizi Syd.) and yellow mosaic (Mungbean Yellow Mosaic Virus) are the major disease under Indian conditions, which causes considerable reduction in yield up to 80 per cent under severe conditions [3]. Further, another major problem in soybean is pods shattering which also reduces yield and in some varieties 100 per cent yield losses have been observed. The extent of yield loss due to pod shattering may range from negligible to significance levels depending upon the time of harvesting, environmental condition and genetic endowment of the variety [11]. Hence screening for soybean genotypes for identifying resistance to above major problems with high yielding potential will help to increase the production to a greater extent.

2. Materials and methods

The material consisted of 84 genotypes of soybean originated from different places of India and abroad. The experiment was laid in augmented design at the Research Farm of Kisan (PG) College, Simbhaoli, Ghaziabad, during kharif, season of 2008. In each replication the genotypes were grown in 2 m long rows with spacing of 40cm × 10cm for row to row and plant to plant, respectively. Within a row, seeds were hand dibbled 10 cm apart. Standard package of practices was followed to raise the crop. Ten competitive plants were randomly selected from each treatment in each replication and data were recorded on 3 qualitative characters namely, pod shattering resistance, rust resistance and yellow mosaic disease resistance.

2.1. Screening for pod shattering resistance

The pod shattering resistance was recorded at physiological maturity of the pod. The screening was done under laboratory condition by following the methodology adopted by IITA [4]. The results were recorded as percentage of pod shattering. IITA method of calculating pod shattering under lab conditions:

A sample of 25 pods were collected and kept in oven at 40°C for 7 days.

On the 7th day the number of shattered pods were counted and expressed in percentage as below,

Number of pods shattered

Pod shattering percentage (%) = x 100

Total number of pods

The genotypes were classified into different categories based on their reaction to pod shattering. The scoring rate was followed according to method adopted by IITA.

Sl.NoCategory Resistant reaction
1.No pod shattering Shattering resistant
2.<25% pod shattering Shattering tolerant
3.25-50% pod shattering Moderately shattering
4.51-75% pod shattering Highly shattering
5.>75% pod shattering very highly shattering

2.2. Screening for rust resistance

The scoring for rust was done just after initiation of flowering and before pod formation. The observations were taken on lower, middle and upper leaves for density of pustule and sporulating intensity. Based on the symptoms, pustule density and sporulation intensity grades were given. The genotypes were later grouped into different categories from immune to highly susceptible. The scale (0-9) used was as follows:

Sl. No.ScaleCategory
1.0Immune
2.1Resistant
3.3Moderately resistant
4.5Moderately susceptible
5.7Susceptible
6.9Highly susceptible

2.3. Screening for yellow mosaic disease resistance

84 soybean genotypes grown in natural (field) conditions at Research Farm of Kisan (PG) College, Simbhaoli, Ghaziabad during kharif, 2008 were screened. Number of plants showing distinct symptoms in each line was counted 60 days after sowing and per cent disease incidence was calculated by using the following formula:

Number of plants infected in a row

Per cent Disease Incidence (PDI) = x 100

Total number of plants in a row

The genotypes were later grouped into different categories from immune to highly susceptible [7]. The scale used was as follows (0-9):

ScaleDescription Category
0No symptoms of plants Immune
11% or less plants exhibiting symptoms resistant
31 to 10% plants exhibiting symptoms moderately resistant
511 to 20% plants exhibiting symptoms moderately susceptible
721 to 50% plants exhibiting symptoms susceptible
951% or more plants exhibiting symptoms highly susceptible

3. Experimental results

3.1. Screening for pod shattering

84 genotypes of soybean were screened for pod shattering resistance in order to identify resistant cultivars during kharif, 2008. The screening was done according to method adopted by IITA, Nigeria. The data presented in Table 1 revealed that pod shattering percentage ranged from 8.7 (Himsoy-1560) to 93.3 per cent (Punjab- 1). The result indicated that there is no variety, which is resistant to pod shattering. However, some of the varieties viz., Bragg, CGP-76, EC-322536, EC-34092, JS 93-05, Lee, MAUS-2, NRC-7, EC-34101, EC-34092, JS 71-05, EC-34101, EC-392536, G-26, Himsoy-1560, Himsoy-1514, Pusa-16, Pusa-22, VLS-1, VLS-2, VLS-47 and the check JS-335 were found to be tolerant. Later these genotypes were grouped into different categories based on IITA, Nigeria scale and the data is presented in Table 2. The results revealed that none of the genotypes were immune or resistant to pod shattering.

Sl.NoGenotypesShattering %GradeSl NoGenotypeShattering %Grade
1.Alankar58.7HS43.EC-39253616.0TO
2.Ankur47.0MS44.EC-39483945.3MS
3.AGS-3459.7HS45.G-4815.0TO
4.AGS-5052.7HS46.G-47935.0MS
5.Bragg15.3TO47.G-48251.7HS
6.Local black soybean78.3VHS48.G-734083.7VHS
7.CO-180.3VHS49.G-2635.7MS
8.CO-257.0HS50.G-5-161.7HS
9.CGP-7615.3TO51.Hardee46.0MS
10.CGP-24862.0HS52.Hara soya17.3MS
11.CGP-203746.0MS53.Himsoya-15608.7TO
12.DSb-146.3MS54.Himsoya-151411.8TO
13.DSb-241.7MS55.Improved pelican83.3VHS
14.DSb-3-444.3MS56.Indira Soya 932.0MS
15.DSb-548.7MS57.C-3950651.7HS
16.DSb-6-137.7MS58.IC-4985956.7HS
17.DSb-756.0HS59.IC-10487746.3MS
18.DSb-845.0MS60.JS-283.0VHS
19.DS-17-535.3MS61.JS-71-0518.0TO
20.EC-10336958.3MS62.JS-72-28036.7MS
21.EC-10992375.0VHS63.JS-72-4444.3MS
22.EC-32253620.7TO64.JS-75-4655.0HS
23.EC-24177836.7MS65.JS-76-20531.9MS
24.EC-24178063.3HS66.JS-80-2167.0HS
25.EC-3409216.0TO67.JS-90-4155.7HS
26.EC-11842061.3HS68.JS-93-10519.0TO
27.EC-3410122.0TO69.JS-87-2531.7MS
28.EC-25144972.7HS70.KB-7930.0MS
29.Lee15.0TO71.Pusa-2032.7MS
30.MACS-1343.0MS72.Pusa-2216.3TO
31.MACS-33072.7VHS73.Pusa-2433.0HS
32.MACS-45057.3MS74.Pusa-3762.3HS
33.MACS-5746.0MS75.Pusa-4074.7HS
34.MAUS-4785.0VHS76.Samrat31.3MS
35.MAUS-6873.0HS77.T-4971.7HS
36.MAUS-219.0TO78.VLS-112.1TO
37.NRC-719.7TO79.VLS-225.3TO
38.NRC-1226.3MS80.VLS-2131.7MS
39.PK-102480.0VHS81.VLS-4717.0TO
40.PK-102932.0MS82.JS-335 (C)10.3TO
41.Punjab-193.3VHS83.KHSb-2(C)43.5MS
42.Pusa-1623.7TO84.Monetta (C)90.7VHS

Table 1.

Screening of soybean genotypes for pod shattering resistance

3.2. Screening for rust resistance

Growing resistant varieties is the most economical and safe method of controlling the rust of soybean, which is a devastating disease resulting in heavy yield loss. In order to identify the resistant cultivars 84 genotypes of soybean were screened for rust resistance during kharif 2008 under natural epiphytotic conditions at Dharwad. The rust incidence was recorded at physiological maturity of the genotypes and the results are presented in Table 3. Reactions of 84 genotypes to rust revealed that, none of the genotypes showed immune reaction to rust. Two genotypes viz., EC 241778 and EC 241780 showed resistant reaction (1 grade), which were considered as resistant and the remaining 82 genotypes as highly susceptible (9 grade).

Sl.No.Category
Resistant reactionNumber ofgenotypesGenotypes
1No pod shatteringShattering resistant00-
2< 25% pod shatteringShattering tolerant20Bragg, CGP-76, EC-322536, EC-34092, JS 71-05, JS-93-05, Lee, MAUS-2, WEC-7, EC-34101, EC-392536, G-26, Himsoya-1560, Himsoya-1514, Pusa-16, Pusa-22, VLS-1, VLS-2, VLS-47, JS-335(C)
325-50% pod shatteringModerately shattering32Ankur, CGP-2037, DSb-1, DSb-2, DSb-3-4, DSb-5, DSb-6-1, DSb-8, PS-17-5, EC-103369, EC-241778, IC-104877, JS 72-280, JS 72-44, JS 76-205, JS 87-25, KB-79, MACS-13, MACS-450, MACS-57, NRC-12, PK-1029, EC-394839, G-48, G-7340, Hardee, Harasoya, Indira soya, Pusa-20, Samrat, VLS-21, KHSb-2 (C).
451-75% pod shatteringHighly shattering21Alankar, AGS-34, AGS-50, CO-2, CGP-248, DSb-7, EC-241780, EC-118420, IC-39506, IC-49859, JS 75-46, JS 80-21, JS 90-41, MAUS-68, EC-251449, G-479, G 5-1, Pusa-24, Pusa-37, Pusa-40, T-49
5>75% pod shatteringVery highly shattering11Local black soybean, CO-1, EC-109923, JS-2, MACS-330, MAUS-47, PK-1024, G-482, Improved pelican, Punjab-1, Monetta (C)

Table 2.

Grouping of Soybean genotypes for pod shattering resistance

Sl.No.ReactionGrade(0-9)Number ofgenotypesGenotypes responded
1Immune000-
2Resistant102EC- 241778, EC- 241780
3Moderately resistant300-
4Susceptible500-
5Moderately Susceptible700-
6Highly Susceptible982Alankar, Ankur, AGS-34, AGS-50, Bragg, Local black soybean, CO-1, CO-2, CGP-76, CGP-248, CGP-2037, DSb-1, DSb-2, DSb 3-4, DSb-5, DSb 6-1, DSB-74, DSb-8, DS 17-5, EC-103369, EC-109923, EC-322536, EC-34092, EC-118420, EC-34101, EC-251449, EC-392536, EC-394839, G-48, G-479, G-482, G-7340, G-26, G-5-1, Hardee, Harasoya, Himsoya-1560, Improved pelican, Indirasoya, IC-39506, IC-49859, IC-104877, JS-2, JS 71-05, JS 72-280, JS 72-44, JS 75-46, JS 76-205, JS 80-21, JS 90-41, JS 93-105, JS 87-25, KB-79, Le, MACS-13, MACS-330, MACS-450, MACS-57, MAUS-47, MAUS-68, MAUS-2, NRC-7, NRC-12, PK-1024, PK-1029, Punjab-1, Pusa-16, Pusa-20, Pusa-22, Pusa-24, Pusa-37, Pusa-40, Samrat, T-49, VLS-1, VLS-2, VLS-21, VLS-47, JS-335 (C), KHSb-2 (C), Monetta (C).

Table 3.

Grouping of soybean genotypes for soybean rust resistance

3.3. Screening for yellow mosaic disease (YMD)

84 genotypes of soybean were screened for yellow mosaic disease under natural conditions at Research Farm of Kisan (PG) College, Simbhaoli, Ghaziabad during kharif, 2008. The data presented in Table 4 revealed that, YMD incidence ranged from 0.95 to 90.12 per cent. Among the 84 genotypes screened lowest incidence was recorded with genotype MACS 57 (0.48%), followed by EC 241778 (0.49%). Genotypes JS 90-41 (90.12) recorded highest incidence followed by JS 76-205 (89.15%) and T 49 (86.21%). All the genotypes and their percent disease incidence are tabulated in Table 5, which categorizes these genotypes based on 0-9 scale into different reaction types. It is evident from the table that none of the genotypes tested were immune or resistant.

Sl.NoGenotypesPDI*ReactionSl NoGenotypePDI*Reaction
1.Alankar9.25MR43.EC-39253627.00S
2.Ankur0.75R44.EC-39483942.12S
3.AGS-348.21MR45.G-4815.25MS
4.AGS-507.68MR46.G-47917.25MS
5.Bragg8.58MR47.G-48225.65S
6.Local black soybean61.25HS48.G-734032.15S
7.CO-13.58MR49.G-269.12MR
8.CO-27.54MR50.G-5-142.12S
9.CGP-7615.61MS51.Hardee39.15S
10.CGP-24819.25MS52.Hara soya33.89S
11.CGP-20379.21MR53.Himsoya-156019.14MS
12.DSb-125.23S54.Himsoya-151440.01S
13.DSb-232.15S55.Improved pelican42.15S
14.DSb-3-415.25MR56.Indira Soya 90.45R
15.DSb-575.25HS57.C-395067.12MR
16.DSb-6-113.25MS58.IC-4985962.15HS
17.DSb-727.85S59.IC-10487775.12HS
18.DSb-826.32S60.JS-246.12S
19.DS-17-516.25MR61.JS-71-0578.98HS
20.EC-1033690.75R62.JS-72-28046.25S
21.EC-10992339.25HS63.JS-72-4429.12S
22.EC-3225360.52R64.JS-75-4689.12HS
23.EC-2417780.49R65.JS-76-2058.81MR
24.EC-2417809.85MR66.JS-80-2190.12HS
25.EC-3409245.25S67.JS-90-4111.85MS
26.EC-11842037.12S68.JS-93-1058.82MR
27.EC-3410113.25MS69.JS-87-257.81MR
28.EC-2514498.25MR70.KB-796.23MR
29.Lee46.3S71.Pusa-207.15MR
30.MACS-135.12MR72.Pusa-220.92R
31.MACS-33081.21HS73.Pusa-248.25MR
32.MACS-4500.89R74.Pusa-377.10S
33.MACS-570.48R75.Pusa-4029.12S
34.MAUS-470.56R76.Samrat36.57S
35.MAUS-6885.12HS77.T-4986.21HS
36.MAUS-231.25S78.VLS-140.25S
37.NRC-70.78R79.VLS-279.85HS
38.NRC-1216.25MS80.VLS-2133.25S
39.PK-10240.85R81.VLS-4730.5S
40.PK-10290.52R82.JS-335 (C)30.96S
41.Punjab-15.23MR83.KHSb-2(C)28.25S
42.Pusa-166.12MR84.Monetta (C)7.6MR

Table 4.

Screening of soybean genotypes for yellow mosaic disease resistance

* Percent disease incidence


ScaleDescriptionCategoryNumber ofgenotypesGenotypes
0No symptoms on plantsImmune00-
11% or less plants
exhibiting symptoms
Resistant12Ankur, EC-103369, EC-322536, EC-241778, Indirasoya 9, MACS-450, MACS-57, MAUS-47, NRC-7, PK-1024, PK-1025, Pusa-22
31-10% plants exhibiting
symptoms
Moderately
resistant
22Alankar, AGS-34, Bragg, AGS-50, CO-1, CO-2, CGP-2037, DSb 3-4, PS-17-5, EC-241780, EC-251449, G-26, IC-39506, JS 80-21, JS 87-25, KB-79, MACS-13, Punjab-1, Pusa-16, Pusa-20, Pusa-24, Monetta (Check).
511-20% plants exhibiting symptomsModerately
susceptible
10CGP-76, CGP-248, DSb-6-1, EC-34101, G-48, G-479, Himory-1560, IC- 49859, JS-93-105, NRC-12
721-50% plants exhibiting symptomsSusceptible28DSb-1, DSb-2, DSb-7, DSb-8, EC-34092, EC-118420, EC-392536, EC-394839, G-7340, G-482, G-5-1, Hardee, Harasoya, Improved pelican, JS 71-05, JS 72-44, JS 75-46, Lee, MAUS-2, Pusa-37, Pusa-40, Samrat, VLS-1, Himsoya-1514, VLS-21, VLS-47, JS-335(C), KHSb-2 (Check)
951% or more plants
exhibiting symptoms
Highly
susceptible
12Local black soybean, DSb-5, EC-109923, IC-104877, JS-2, JS 72-280, JS 76-205, JS 90-41, MACS-330, MAUS-68, T-49, VLS-2

Table 5.

Grouping of genotypes into different categories for soybean yellow mosaic virus resistance

4. Discussion

4.1. Screening for pod shattering resistance

Pod shattering is one of the major constraints in soybean, which reduces the yield potential considerably. So management of pod shattering is of great importance for achieving higher productivity. Hence, the identification of resistant sources for pod shattering is one of the most important aspect in the management of pod shattering. In the present study 84 genotypes of soybean were screened for pod shattering resistance under lab condition. The pod shattering values ranged from 8.7 to 93.3 per cent. JS-335 one of the most popular variety recorded as tolerant with mean pod shattering value of 10.3 per cent. It is evident from the table that, none of the genotypes were better than the JS-335 except Himsoy-1560, which recorded 8.7 per cent mean pod shattering value. Among 84 genotypes, 20 genotypes fall under tolerant category and 32 under moderately shattering. Fifteen Indian soybean varieties were screened for pod shattering resistance and out of these three varieties viz., JS-1515, JS-1608 and JS-1625 were found resistant against pod shattering [16]. Similarly, while screening for pod shattering resistance, Bragg and JS-71-05 recorded the lowest pod shattering and Punjab-1 with highest pod shattering value [12]. Similar results were also reported [1, 13].

4.2. Screening for rust resistance

Among many of the diseases in soybean, rust is the major fungal disease which may reduce the yield drastically. So identification of resistant sources and involving them in resistant breeding forms one of the criteria in resistant breeding programme. In the present study 84 genotypes of soybean were screened for rust resistance under natural epiphytotic condition. None of the genotypes showed immune reaction. However, genotypes EC-241778 and EC-241780 showed resistance reaction. Remaining all genotypes exhibited highly susceptible reaction. In general, over all disease incidence was very high. Similar results are reported in [9], who evaluated several soybean genotypes and varieties under natural epiphytotic condition and reported EC-392530, EC-392538, EC-392539, EC-392541, SL-423, RSC-1, RSC-2, JS-80-21 and PK-1029 as moderately resistant. Hundekar (1999) also evaluated S-22, WC-12 and 92-10 as rust resistant germplasm. Among varieties PK-1162, PK-1029, JS-80-21 and PK-1024 showed moderately resistant reaction with better yield. Basavaraja (2002) identified three useful mutants which are moderately resistant to rust among 270 induced mutant families studied in M3 generation. Similar results were also reported by various researchers [8, 10, 14, 15]

4.3. Screening for yellow mosaic disease

Yellow mosaic is one of the major viral diseases in India and it is causing major problem during rabi/summer in Utter Pradesh in recent years. The yield loss due to disease may range from minor to complete loss depending upon severity. So identification of resistant sources will help in optimum management and thus help in future breeding programmes. In the present study, 84 genotypes of soybean were taken for screening against yellow mosaic disease under natural conditions. None of the genotypes tested were immure to the disease. Over the entire disease incidence was high which was evident from the results as most of the genotypes fall under the category moderately susceptible to susceptible. Similar results were also reported [6, 17]. They screened 88 indigenous and exotic soybean genotypes in the field and found EC-107014, EC-107003 and EC-100777 resistant.

© 2013 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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M. H. Khan, S. D. Tyagi and Z. A. Dar (February 13th 2013). Screening of Soybean (Glycine Max (L.) Merrill) Genotypes for Resistance to Rust, Yellow Mosaic and Pod Shattering, Soybean - Pest Resistance, Hany A. El-Shemy, IntechOpen, DOI: 10.5772/54697. Available from:

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