Open access peer-reviewed chapter

Biofilm Formation of Salmonella

By Daxin Peng

Submitted: October 23rd 2015Reviewed: March 7th 2016Published: July 13th 2016

DOI: 10.5772/62905

Downloaded: 2462


Salmonella spp. may form biofilm, and bacteria in biofilm are more resistant to drug, chemical, physical and mechanical stresses, and host immune system. The progress on biofilm research will be helpful for the development of new tools and strategies to prevent biofilm-related disease and decontaminate biofilm-derived Salmonella in food production. In this review, we present a comprehensive overview of biofilm formation in Salmonella, included that (1) the component of Salmonella biofilm, (2) the detection methods for biofilm, (3) the identification of biofilm-formation-associated genes, (4) the regulation mechanism of biofilm formation, and (5) virulence or resistance of Salmonella in biofilm.


  • Salmonella
  • biofilm
  • component
  • mechanism
  • gene
  • pathogenicity
  • drug resistance

1. Introduction

Salmonella entericis an intracellular gram-negative pathogen that infects various hosts, which is classified into more than 2500 serovars [1]. Many serovars, such as those most commonly associated with human infections, including Salmonella enteritidis, Salmonella typhimurium, have a broad host range [2]. In contrast, other serovars, such as Salmonella typhi, Salmonella paratyphi, Salmonella gallinarum, Salmonella choleraesuis, Salmonella abortusovis, and Salmonella dublin, have restricted host ranges and are associated primarily with one or a few hosts [3]. Salmonellacan cause disease in domestic animals, ranging in severity of asymptom, diarrhea and enteritis to systemic syndrome, and result in a huge economic loss in pig and poultry industry. Salmonellosis is also a growing public health concern in both the developed and developing countries, since nontyphoidal Salmonelladisease, a major cause of diarrheal disease globally, is estimated to cause 93 million enteric infections and 155,000 diarrheal deaths each year [4]. The illnesses and outbreaks are most commonly attributed to exposure to contaminated food, and the eggs, broiler chickens, and pigs are among the top sources [5]. Salmonellaoften exist not only as planktonic cells but also as sessile, multicellular forms such as biofilms attached to surfaces. Biofilm formation is important for the spread of Salmonellabecause bacteria in the biofilm are resistant to disinfectants and chemical, physical, and mechanical stresses [68]. The biofilm formation is also contributed to Salmonellavirulence, since bacteria in the biofilm are more resistant to antibiotics and host immune system, resulting in a chronic infection and the development of Salmonellacarrier state [9, 10]. In our review, we present a comprehensive overview of biofilm formation in Salmonella.


2. The component of Salmonellabiofilm

The biofilm formation is a multistep developmental process that always has several distinguishable steps: (a) attachment to the carrier surface, reversible, (b) irreversible attachment, binding to the surface with the participation of adhesions or exopolysaccharides, (c) the development of microcolonies, a distinct mushroom shape, (d) the maturation of biofilm architecture [11, 12], (e) under favorable conditions, the synthesis of martrix compounds decreases and the matrix is enzymatically cleaved, leading to biofilm dispersion [13]. In natural environments, Salmonellaforms biofilms on plant [14], abiotic surfaces, including plastics, metal and glass [1517], meat and meat-processing environments [18, 19]. In addition, Salmonellacan colonize gallstones under laboratory conditions [20], and the Salmonellabiofilm can be directly visualized by confocal micrographs of extracellular matrix on the surface of human cholesterol gallstones [21]. They can also form biofilms on chicken intestinal epithelium [22] or HEp-2 cells that are suspended in once-flow-through continuous culture conditions [23].

The extracellular matrix of Salmonellabiofilm is majorly composed of curli (amyloid fimbriae), cellulose [24, 25], biofilm-associated protein (Bap) [26], O-antigen capsule [14, 27], extracellular DNA [28, 29]. The expression pattern of the biofilm is serovar specific and correlates with contact surface [30]. Curli were first discovered in the late 1980s on Escherichia colistrains that caused bovine mastitis, and they are mainly involved in adhesion to surfaces, cell aggregation and biofilm formation. Curli also mediate host cell adhesion and invasion, and they are potent inducers of the host inflammatory response [12]. The curli protein is encoded by the divergently transcribed csgBAC(agfBAC) and csgDEFG(agfDEFG) operons [31, 32]. The csgBACoperon encodes the major structural subunit, CsgA, and the surface-exposed nucleator protein CsgB. A third gene, csgC, is in the csgBACoperon, but no transcript for csgChas been detected in curli biogenesis [32]. The other study shows that both CsgC and CsgE facilitate extracellular thin aggregative fimbriae synthesis in Salmonella enteritidis[33]. The csgDEFGoperon encodes accessory proteins required for curli assembly. The csgDgene encodes a transcriptional regulator belonging to the LuxR family, CsgD, for active transcription of csgBACpromoter [24]. Although Giaouris et al. [34] found that CsgF was expressed in biofilm growth when compared with planktonic and biofilm cells of Salmonella enteritidison stainless steel surface, the function of csgFand csgGgenes has not been resolved in Salmonella. Cellulose is a polysaccharide composed of β(1 → 4)-linked D-glucose units [35], which is an important exopolysaccharide normally synthesized in the Salmonellabiofilms. The production of cellulose and curli by Salmonellaleads to a matrix of tightly packed cells covered in a hydrophobic network. The operons, bcsABZDand bcsEFG, are required for cellulose biosynthesis [36]. Cellulose biosynthesis is positively regulated by CsgD, which stimulates the transcription of AdrA that harbours a cytoplasmic GGDEF domain. AdrA activates cellulose production on the post-transcriptional level either by direct interaction with bcsoperons or indirect interaction with bis-3′-5′-cyclic dimeric guanosine monophosphate (c-di-GMP) [25, 37, 38]. BapA, a large cell-surface protein required for biofilm formation, is encoded by bapAgene and secreted through a type-I protein secretion system (bapBCDoperons) situated downstream of the bapAgene. The expression of bapAis coordinated with that of genes encoding curli fimbriae and cellulose, through the action of csgD[26, 39]. The bapAgene is also highly conserved in Salmonella[40]. Salmonellaproduces an O-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. The operons yihU-yshA and yihVW are responsible for capsule assembly and translocation [41] and regulated by CsgD. Although the O-antigen capsule do not appear to be important for multicellular behavior, they play an important role in attachment and environmental persistence [14]. However, the O-antigen capsule is required for biofilm formation of Salmonella typhimuriumand Salmonella Typhion cholesterol gallstones, and the operons are regulated in a csgD-independent manner [42]. Extracellular DNA is shown to be a matrix component of Salmonellabiofilms cultivated in flow chambers and on glass surfaces [28]. However, the presence of extracellular DNA plays an inhibitive and destabilizing effect during biofilm development of Salmonellaon abiotic surfaces [29].

3. The detection methods for biofilm

3.1. Quantification of biofilm formation

Biofilm formation of Salmonellacan be quantitated by microplate-based crystal violet staining [43]. Briefly, the overnight broth cultures of bacterium are diluted 1:100 in the diluted tryptic soy broth (TSB). One hundred µl of bacterial suspension is added into 96-well U-bottomed polystyrene microtiter plates. Plates are incubated at 28°C for 24 h under static conditions. Then, non-adherent bacteria are removed and the wells are washed gently three times with 200 µl of distilled water. One hundred μl of 0.4% crystal violet (v/v) is added into each well and stained for 20 min. After discard of staining liquid, all loosely adhering bacteria and dye are gently washed off with distilled water for three times. The dye bound to the adherent cells is solubilized with 100 µl of anhydrous ethanol per well. The optical density (OD) is measured at 590 nm, and OD value of biofilm-formation strain is significantly higher than that of negative control. It provides more reproducible results with an addition of a fixtion step (80°C for 30 min) prior crystal violet staining [19]. Combined with resazurin assay, the number of metabolically active cells is able to be evaluated [44]. With wheat germ agglutinin-Alexa Fluor 488 conjugate, which selectively binds to N-acetylglucosamine residues in biofilms, the spectrofluorometric assay provides a more sensitive method for quantification and characterization of bacterial biofilms [45].

3.2. Biofilm formation in glass tube

The overnight cultures of bacteria are diluted 1:100 in the diluted TSB. Two milliliters of each bacterial suspension are added into borosilicate glass tubes and incubated at 28°C for 48 h. Then, the liquid is decanted and the tubes are washed gently three times with distilled water. Two ml of 0.4% crystal violet (v/v) are added into each tube and stained at room temperature for 20 min. The stained biofilm is observed at the liquid–air interface on the glass test tube walls or at the bottom of the tube [46]. The glass tubes may also be incubated at 37°C at 200 rpm by using an orbital shaker, and biofilm is observed at interphase without staining [47].

3.3. Congo red/carbol fuchsin staining

The overnight culture (1:100 diluted in TSB) is inoculated into 3 ml of fresh TSB in a 6-well plate containing sterile polystyrene coverslip (20 × 20 mm). After incubation at 28°C for 24 or 48 h without agitation, the coverslips are removed carefully, treated with cetylpyridinium chloride (10 mM) for 30 s, rinsed with distilled water and air dried for 20–30 min. After fixation by gentle heating, the coverslips are stained with a mixture of saturated aqueous Congo red solution and 10% Tween-80 (2:1, V/V) for 30 min and rinsed with distilled water. After staining with 10% (v/v) Ziehl carbol fuchsin for 6 min and rinsing in distilled water, the coverslips are air dried and mounted on slides [48]. Under a light microscope, bacterial cells on slides show purple staining, while the exopolysaccharides of biofilm show pink staining [46].

3.4. Field emission scanning electron microscopy

The coverslips with cultured bacteria are fixed in 3% glutaraldehyde in 0.1 M phosphate-buffered saline at 4°C for 2 h. The samples are then dehydrated with increasing concentrations of ethanol (50, 70, 80, 90, and 100%) followed by isoamyl acetate (100%), each for 15 min. The samples are critical point dried for 5 h, coated with gold palladium alloy, and observed under a field emission scanning electron microscope [49]. The biofilm-formation strain exhibits increased clusters of bacteria cells with curli fimbriae and has meshwork-like structures surrounding the cell surfaces.

3.5. Congo red and calcofluor plates

LB agar plate without salt supplemented with 40 mg/L Congo red and 20 mg/L brilliant blue is used to determine the Congo red-binding property of the colonies. LB agar plate supplemented with 200 mg/L calcofluor (fluorescent brightener) is used to determine the cellulose production by comparing the fluorescence of the test strains under UV light [49]. Biofilm of Salmonellais mainly composed of curli and cellulose, and Salmonellastrains were grouped into distinct morphotypes according to Congo red binding: (a) red, dry, and rough indicating curli and cellulose production (RDAR), (b) brown, dry, and rough, indicating a lack of cellulose synthesis (BDAR), (c) pink, dry, and rough, indicating a defect in curli expression (PDAR), (d) smooth, brown, and mucoid, indicating a lack of cellulose synthesis but overproduced capsular polysaccharide (SBAM), and (e) smooth and white, indicating a lack of both curli and cellulose production (SAW) [19, 31, 50].

3.6. Confocal laser microscopy

Bacteria cultured on coverslipes, dish, or microplate are stained by 0.1 M phosphate-buffered saline (pH 7.2) containing SYTO 9 and propidium iodide. After 10 min incubation in the dark at room temperature, stained samples are examined using a confocal scanning laser microscopy. Fluorochromes are excited using an argon laser source at 488 nm. Images are collected in two channels, 490–515 and 620–640 nm, corresponding to the emission maxima for SYTO 9 and propidium iodide, respectively. Optical sections approximately 1 μm in height are collected starting from below the focal plane to upward through the entire biofilm. The biofilm cells are clearly observed in a multilayer community [20, 51].

4. Identification of biofilm-formation–associated genes

The most common biofilm-formation–associated genes are the genes encode adhesins. The best characterized of the Salmonellafimbriae is type-1 fimbriae. This fimbrial type is encoded by the fim gene cluster and is assembled by the chaperone–usher system [52]. The fimA gene encodes the major structural subunit, while the fimH gene encodes the adhesin protein that is located at the tip of the assembled fimbrial structure and mediates binding to the receptor. The FimH adhesin is involved in biofilm formation on HEp-2 tissue culture cells, murine intestinal epithelium, and chicken intestinal epithelium [22, 23]. The long polar fimbriae (Lpf) are encoded by the lpfABCDEgenes and have been implicated in the colonization of the murine intestinal mucosa [53, 54]. Plasmid-encoded fimbriae (Pef) are encoded on the 90-kb Salmonellavirulence plasmid and are majorly encoded by pefBCD, orf5, and orf6 genes. Both Lpf and Pef contribute to the early steps of biofilm formation [55]. Salmonella enteritidisproduce a variety of potentially adherent fimbrial types including SEF14 (SefA), SEF17 (CsgA), SEF18 (SefD), and SEF21 (type I, FimA), the role of each fimbrial in biofilm formation is different. The SEF17 encoded by csgAgene stabilize cell–cell interactions during biofilm formation, while SEF21 fimbriae may involve cell surface adherence [56]. SadA is trimeric autotransporter adhesin of Salmonella typhimurium, the expression of SadA resulted in cell aggregation, biofilm formation, and increased adhesion to human intestinal Caco-2 epithelial cells [57]. Salmonellamay persist on post-harvest lettuce during cold storage, the genes stfC, bcsA, misL, and yidR, encoding a fimbrial outer membrane usher, a cellulose synthase catalytic subunit, an adhesin of the autotransporter family expressed from the Salmonellapathogenicity island-3, and a putative ATP-/GTP-binding protein, respectively, have a role in persistence of the pathogen. The bcsA, misL, and yidRknockout mutants are impaired in attachment and biofilm formation, suggesting that these functions are required for biofilm formation [58].

Salmonellaflagella are not required for the formation of the multicellular morphotype on plates. However, the global behavior of the bacterial community on air–liquid, surface–liquid, or cell–liquid interfaces is changed in the absence of flagella. In a mutant lacking flagella and thin aggregative fimbriae, the contribution of the latter to the multicellular morphotype is dominant [59]. Biofilm formation of an flgK mutant in meat and poultry broths and their attachment on surfaces of stainless steel and glass are significantly reduced compared with that of the wild-type strain, suggest that expression of flagella could be involved in biofilm formation and attachment of Salmonellaon contact surfaces [60]. The presence of the flagellar filament enhances binding and biofilm formation in the presence of bile, while flagellar motility and expression of type-1 fimbriae were unimportant in biofilm formation on cholesterol gallstones [61].

Pathogenicity islands accommodate large clusters of genes that contribute to a particular virulence phenotype. Salmonellapossess at least seven salmonellapathogenicity islands (SPIs). Among these, SPI1 is primarily required for bacterial motility and invasion of host cells. Salmonella typhimuriumcultures containing cloned SPI-1 display an adherent biofilm and cell clumps in the media. This phenotype was associated with hyper-expression of SPI-1 type-III secretion functions. Surprisingly, mutations in genes essential for known bacterial biofilm pathways (bcsA, csgBA, bapA) did not affect the biofilms formation, indicating that this phenomenon is independent of established biofilm mechanisms [62]. Salmonellabiofilm cells exposed to superheated steam show decreased transcription of flagella and SPI-1 genes, respectively, whereas increased transcription of SPI-2 genes, important for bacterial survival and replication inside host cells, is detected [63]. In contrast, when compared biofilms of Salmonella typhimuriumwith planktonic cells, the most highly downregulated genes in the biofilm are located on SPI-2 and that a functional SPI2 secretion system regulator (ssrA) is required for Salmonella typhimuriumbiofilm formation. Genes involved in tryptophan (trp) biosynthesis and transport are upregulated in the biofilm. Deletion of trpEresults in decreased bacterial attachment and biofilm formation, indicating that aromatic amino acids make an important contribution to biofilm formation [64]. The aromutants of Salmonellaare frequently used as live vaccines for the oral vaccination of domestic animals, and they are unable to synthesize chorismate, which is a key intermediate in the synthesis of aromatic amino acids. The aromutants exhibit a decreased production of cellulose, N-acetyl-D-glucosamine, or N-acetylneuraminic acid-containing capsular polysaccharide and fimbriae, which explains their inability to form biofilms [65].

Lipopolysaccharide (LPS) synthesis also involves the biofilm formation of Salmonella. Two Tn5 insertion mutations in genes that are involved in ddhCand waaGresult in diminished expression of colony rugosity. Both mutants have impaired biofilm formation when grown in rich medium with low osmolarity, they constitutively form larger amounts of biofilms when the growth medium was supplemented with either glucose or a combination of glucose and NaCl [49]. The rfbAgene also involve in lipopolysaccharide biosynthesis. Biofilm formation by the rfbAmutant in meat and poultry broths and their attachment on surfaces of stainless steel and glass is significantly reduced [60]. Using transposon mutagenesis, the genes metE, ompR, rpoS, rfaG, rfaJ, rfaK, rfaP, rfbH, rhlE, spiA, and steBare found to be associated with biofilm formation of Salmonella enteritidis[66, 67]. When eight mutants with knockout of genes ompR, rpoS, rfaG, rfbH, rhlE, metE, spiA, or steBfrom the Salmonella pullorumare constructed. Only the ompRmutant showed a complete loss of production of curli and biofilm formation. The other mutants showed a modified production of curli and cellulose with less effect related to biofilm formation [68]. Therefore, an integral LPS, at both the O-antigen and core polysaccharide levels, are important in the modulation of curli protein and cellulose production, as well as in biofilm formation.

5. Regulation mechanism of biofilm formation

Biofilm formation is majorly regulated by CsgD protein, a regulator belonging to the LuxR family [69]. CsgD has an N-terminal receiver domain with a conserved aspartate (D59) as a putative target site for phosphorylation and a C-terminal LuxR-like helix-turn-helix DNA binding motif. The unphosphorylated CsgD directly binds the csgBAand adrApromoter regions to activate transcription [70]. Multiple factors bind to the promoter sequence of csgDand regulate its transcription, such as OmpR, RpoS, RpoE, integration host factor (IHF), histone-like nucleoid structuring protein (H-NS), and MlrA. OmpR is one of first discovered to be required for csgDtranscription [71]. Six binding sites (D1–D6) for OmpR are identified in csgDpromoter regions. Binding of OmpR-P to D2 centered immediately upstream of D1 is proposed to repress promoter activity. IHF competes with OmpR-P for binding at its upstream site IHF1, which overlaps with D3–D6 and thereby activate the transcription of csgD[72]. The mutant of ompRin Salmonella enteritidisand Salmonella pullorumhas inability to produce cellulose, curli, and biofilm [68, 73]. RpoS, encodes an alternative sigma factor of RNA polymerase, is critical for bacterial endurance under the most-stressful conditions, including stationary-phase entrance and host adaptation. RpoS is required for transcriptional activation of the csgDpromoter in Salmonella typhimuriumstrains that rdar morphotype are normally expressed at low temperature [31]. However, in two Salmonella typhimuriumstrains, spontaneous mutants are found forming rdar colonies independent of temperature, the regulation of csgDis independent of rpoS[71]. Partially independent of rpoSfor regulation of csgDis observed in Salmonella enteritidis. The rpoSmutant in Salmonella pullorumalso shows similar biofilm forming ability as the wild-type strain [68], suggests that another sigma factor may recognize the csgDpromoter. RpoE is an another regulator in the expression of thin aggregative fimbriae in Salmonella[74], since the rpoEdeletion mutant shows significantly reduced amounts of csgDexpression and modulated biofilm formation. Compared the expression of six different Sigma factors during biofilm formation in a rpoS-independent biofilm-formation strain, the expression of rpoEgene was the highest, and the rpoEmutant could not produce biofilm [75]. Therefore, RpoE acts as a regulator for csgDexpression. IHF is a histone-like heterodimeric protein composed of two homologous subunits. IHF interacts with a define DNA sequence that has a supportive A-tract upstream of the consensus sequence by binding to the minor groove of the DNA. The ihfmutants show altered and reduced biofilm morphotypes on Congo Red agar plates [72]. H-NS prefers to bind AT-rich sites in the intergenic csgBACand csgDEFGregions and causes moderate activation of csgDpromoter. The inactivation of hnsgene result in reduced expression of the rdar morphotype on agar plate [72]. MlrA (MerR-like regulator) acts directly or indirectly on the csgDpromoter, the mlrAmutants of Salmonella typhimuriumno longer produce curli or rugose colony morphology. However, inactivation of mlrAdid not affect curli production and aggregative morphology in an upregulated curli producing Salmonella typhimuriumderivative containing a temperature- and RpoS-independent csgDpromoter region. Therefore, MlrA acts as a positive regulator of RpoS-dependent curli and extracellular matrix production by Salmonella typhimurium[76].

c-di-GMP is recognized as a ubiquitous bacterial second messenger and a key regulator in bacterial transition from a motile and planktonic to a sessile and biofilm lifestyle. High intracellular c-di-GMP levels promote extracellular matrix production and subsequent biofilm formation and repress motility, whereas low intracellular c-di-GMP levels suppress matrix production and promote single-cell motility [77]. The synthesis/degradation of c-di-GMP depends on diguanylate cyclase/phosphodiesterase enzymatic activities. The cyclase activity, which converts two molecules of GTP to c-di-GMP, is encoded in the GGDEF protein domain, while phosphodiesterase activity, which hydrolyzes c-di-GMP to linear 5′-pGpG or two GMP molecules, is encoded in the EAL and HD-GYP domains. For example, Adar, containing a GGDEF domain, encodes diguanylate cyclase synthesizing c-di-GMP, is required for cellulose production and biofilm formation. In another seven GGDEF family (GcpA-G), only GcpA and GcpE are critical for biofilm formation [37]. The EAL domain protein STM4264, STM3611, and the GGDEF-EAL domain protein STM1703 play a determinative role in the expression level of multicellular behavior of Salmonella typhimurium[78, 79]. In contradiction, the EAL-like protein STM1697, neither degrade nor bind c-di-GMP, promotes biofilm formation and CsgDexpression through interaction with proteins that regulate flagella function [80]. High intracellular amounts of c-di-GMP in Salmonella typhimuriuminhibited invasion and abolished induction of a pro-inflammatory immune response in the colonic epithelial cell line HT-29. Inhibition of the invasion and IL-8 induction phenotype by c-di-GMP requires the major biofilm activator CsgD and/or BcsA. Therefore, c-di-GMP signaling is at least equally important in the regulation of Salmonella–host interaction as in the regulation of biofilm formation at ambient temperature [81].

CsgD synthesis is also regulated at the post-transcriptional level by sRNA. sRNAs have emerged as a diverse group of trans- or cis-encoded regulatory molecules of approximately 50–250 nt in size. The RNA chaperone Hfq protects sRNAs form degradation and facilitates their binding to the target mRNAs. All these sRNA may negatively regulate csgDgene expression by binding to the overlapping 5′-region of the transcript, masking the ribosome binding site, resulting in the inhibition of translation or the degradation of mRNA [82]. In Escherichia coli, sRNAs, OmrA/B, McaS, RprA, and GcvB are identified, which downregulate CsgDtranslation [83]. In E. coliand Salmonella, RydC’s 5′-domain interacts with csgDmRNA translation initiation signals to prevent initiation, stimulation of RydC expression reduces biofilm formation by impairing curli synthesis [84]. Surprisingly, two Hfq-dependent sRNAs (ArcZ and SdsR) are responsible for positively regulation of rdar morphotype expression in Salmonella typhimurium[85]. Salmonellabiofilm development depends on the phosphorylation status of RcsB. The unphosphorylated RcsB is essential to activate the expression of the biofilm matrix compounds. The inhibition of biofilm development by phosphorylated RcsB is due to the repression of CsgDexpression, through a mechanism dependent on the accumulation of the sRNA RprA [86].

Many gram-negative bacteria utilize N-acyl-L-homoserine lactones (AHLs) to bind to transcriptional regulators leading to activation or repression of target genes. Salmonellado not synthesize AHLs but do contain the AHL receptor, SdiA. The Salmonella sdiAgene regulates the rckgene, which mediates its adhesion and invasion of epithelial cells and the resistance of the organism to complement [87]. The rckgene is located on the virulence plasmid of pRST98, AHLs increase rckexpression in pRST98-carrying strains, thereby enhancing bacterial adherence, serum resistance, and bacterial biofilm formation [88].


6. Virulence or resistance for biofilm

Biofilm formation may involve in the virulence of Salmonella. Salmonella enteritidisstains isolated from either the environment, dairy products, or infected patients are divided into two groups on the basis of their virulence (50% lethal dose) in chickens infected intraperitoneally. Only the virulent strains produce aggregates and formed visible filaments attached to the glass tube [47]. Further study confirms that the virulence of the biofilm-producing strain in infected chickens increases proportionally to the amount of stored glycogen, suggesting a possible role of the glycogen depot in the virulence of Salmonella enteritidis[89]. When tested for infection in Caco-2 cells and HEp-2 cells, the more virulent strains of Salmonella enteritidis, which are biofilm producers in adherence test medium, are able to disrupt monolayers. In contrast, the low-virulence strains of Salmonella enteritidis, which do not produce biofilms in adherence test medium, have no effect on the same cells. The high-virulence Salmonella enteritidisstrains incubated under optimum biofilm-forming conditions may release a soluble factor, which enables the disruption of the integrity of Caco-2 monolayers [90]. The relationship between biofilm-forming ability and the pathogenicity is also evaluated in Salmonella pullorum. Although the virulence of Salmonella pullorumstrains is independent of their ability of biofilm formation, prior growth as a biofilm for a biofilm producer of Salmonella pullorumleads to enhanced virulence in chickens, suggested that biofilm formation may be one of important virulence factor for Salmonella pulloruminfection [46].

The csgBACoperon is required for curli biosynthesis in Salmonella. The csgAmutation is not reduced in ability to attach or colonize alfalfa sprouts, whereas the csgBmutation is reduced. Thus, csgBalone can play a role in attachment of Salmonellato plant tissue [91]. Competitive infection experiments in mice shows that csgAmutant cells outcompeted rdar-positive wild-type cells, indicating that aggregation via the rdar morphotype is not a virulence adaptation in Salmonella typhimurium. Furthermore, in vivo imaging experiments show that thin aggregative fimbriae genes are not expressed during infection but are expressed once Salmonellawas passed out of the mice into the feces [92]. However, Salmonella typhimuriumstrains isolated from water buffalo calves affected by lethal gastroenteritis are tested in vivo in a mouse model of mixed infection. The most pathogenic strain is characterized by a high number of virulence factors and the presence of the locus csgA, coding for a thin aggregative fimbria [93].

The bcsABZCand bcsEFGoperons are required for cellulose biosynthesis in Salmonella. Bacterial adherence and invasion assays of eukaryotic cells and in vivo virulence studies of cellulose-deficient mutants of bcsCand bcsEgenes indicate that the production of cellulose is not involved in the virulence of Salmonella enteritidis. However, cellulose-deficient mutants are more sensitive to chlorine treatments, suggesting that cellulose production and biofilm formation may be an important factor for the survival of S. enteritidison surface environments [36]. Salmonella typhimuriummakes cellulose when inside macrophages. An attenuated mutant lacking the mgtCgene exhibits increased cellulose levels due to increased expression of the cellulose synthase gene bcsAand of cyclic diguanylate, the allosteric activator of the BcsA protein. Inactivation of bcsArestore wild-type virulence to the Salmonella mgtCmutant, indicating that Salmonellapromotes virulence by repressing cellulose production [94].

BapA, a large cell-surface protein, is required for biofilm formation by Salmonella. Studies on the contribution of BapA to Salmonella enteritidispathogenesis reveal that orally inoculated animals with a bapA-deficient strain survived longer than those inoculated with the wild-type strain. Also, a bapAmutant strain showed a significantly lower colonization rate at the intestinal cell barrier and consequently a decreased efficiency for organ invasion compared with the wild-type strain [26]. Osmoregulated periplasmic glucans (OPGs) are major periplasmic constituents of Gram-negative bacteria. An opgGHmutant strain in Salmonella typhimurium, which is defective in OPG biosynthesis, severely impaires biofilm formation. The opgGH mutant strain poorly colonizes mouse organs when introduced orally along with the wild-type strain [95].

Besides, the constitutional components of biofilm, there are many regulation proteins involved in both biofilm formation and virulence. An ompRmutant of Salmonella enteritidishas no ability to produce cellulose, curli, and biofilm and shows similar adherence percentage to and invasion percentage of epithelial cells as wild-type strain. Intraperitoneal challenge of bacteria in BALB/c mice reveals that the ompRmutant strain is significant attenuated [73]. A spiAgene mutant shows reduced biofilm formation and significantly decreased curli production, and reduced intracellular proliferation of macrophages during the biofilm phase. In addition, the spiAmutant was attenuated in a mouse model in both the exponential growth and biofilm phases [67]. Deletion of genes ompRand spiAin Salmonell pullorumstrains contribute to attenuation of virulence in 1-day-old chickens [68]. DksA is a conserved gram-negative regulator that binds directly to the RNA polymerase secondary channel. In Salmonella typhimurium, expression of the dksAgene is induced during the logarithmic phase and DksA plays an important role in motility and biofilm formation. DksA positively regulates the Salmonellapathogenicity island 1 and motility-chemotaxis genes and is necessary for Salmonella typhimuriuminvasion of human epithelial cells and uptake by macrophages. The dksAgene is induced at the midcecum during the early stage of the infection and required for gastrointestinal colonization and systemic infection in a colitis mouse model [96].

Salmonellain biofilm is resistant to antibiotic. One of key mechanisms of antibiotic resistance is efflux. There are five families of multidrug resistance (MDR) efflux pumps, in which the AcrAB–TolC efflux system is the best characterized MDR system. Ten mutants of Salmonella typhimuriumlacking MDR efflux systems, such as tolC, acrB, acrD, acrEF, mdtABC, mdsABC, emrAB, mdfA, mdtK, and macABare compromised in their ability to form biofilms. The mutants expressed significantly less csgBor csgDthan wild type, indicating that loss of all multidrug resistance efflux pumps of Salmonella typhimuriumresults in impaired ability to form a biofilm [97]. Further study confirms that mutants of Salmonella typhimuriumthat lack TolC or AcrB, but surprisingly not AcrA, are compromised in their ability to form biofilms. The biofilm defect results from transcriptional repression of curli biosynthesis genes and consequent inhibition of production of curli. Therefore, the inhibition of efflux is a promising antibiofilm strategy [98]. However, recent studies offer contradictory findings about the role of multidrug efflux pumps in bacterial biofilm development. When no selective pressure is applied, Salmonella typhimuriumis able to produce biofilms even when the AcrAB efflux pumps are inactivated. Upon exposure to chloramphenicol, the formation of biofilms on solid surfaces as well as the production of curli are either reduced or delayed more significantly in both AcrA and AcrAB mutants, implying that the use of efflux pump inhibitors to prevent biofilm formation is not a general solution and that combined treatments might be more efficient [99]. Triclosan is a potent biocide that is included in a diverse range of products. Salmonellabiofilm-derived cells are more resistant to Triclosan. Within biofilms, triclosan upregulate the transcription of acrAB, marA, bcsA, and bcsEgenes. Thus, Salmonellawithin biofilms could experience reduced influx, increased efflux and enhanced exopolysaccharides production. The data suggest that tolerance of Salmonellatowards triclosan in the biofilm is attributed to low diffusion through the extracellular matrix, while changes of gene expression might provide further resistance to triclosan and to other antimicrobials [100].

In summary, Salmonellabiofilm formation is major controlled by CsgD regulatory network and regulated by multiple transcriptional factors, c-di-GMP, and sRNAs. More and more genes are found to be associated with both biofilm formation and virulence. Dissection of their function and relationship will helpful for development of new tools and strategies to prevent biofilm-related disease and decontaminate biofilm-derived Salmonellain food production.

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Daxin Peng (July 13th 2016). Biofilm Formation of Salmonella, Microbial Biofilms - Importance and Applications, Dharumadurai Dhanasekaran and Nooruddin Thajuddin, IntechOpen, DOI: 10.5772/62905. Available from:

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