1.1. Ocular anatomy
In order to understand the pathophysiology of this condition we will describe the
Stroma: a thin avascular layer with fibroblasts and melanocytes. It is heavily pigmented in persons with brown eyes less pigmented in green and hazel irises and least in blue. Posteriorly, the stroma contains the sphincter pupillae muscle (parasympathetic, miosis).
Pigment epithelium: consists of 2 layers of cells: -anterior layer, which intermingles with the dilator pupillae muscle (sympathetic, mydriasis) -posterior layer, which is continuous with the pigment epithelium of the ciliary body and RPE of the retina, all having the same embryologic origin.
1.2. Difference between ocular melanoma and cutaneous melanoma
Ocular and cutaneous melanomas show several differences despite they both derive from melanocytes. Both malignancies show a high tendency to metastasize though they display different preferential sites. Skin melanomas spread to distant skin sites, lung, liver, central nervous system and bone. However uveal melanoma, the most frequently diagnosed of the ocular melanomas, gives rise to metastases almost exclusively in the liver which is affected in 90% of the cases.
Interestingly, both malignancies display similar chromosomal aberrations as well as a similar gene expression profile . The similarity in this aspect, despite the difference in tumor behaviour, serves as a proof of the role of the microenvironment in tumor development.
With respect to the early diagnosis, in the skin melanoma the suspected diagnosis and the subsequent clinical follow-up are based on the ABCDE rule.On the other hand, in the diagnosis of ocular melanoma the most relevant information comes from the ophthalmoscopy and the ultrasonography. Finally, consulting times for patients and prognosis are different for both types of melanoma.
In this chapter we will focus in the clinical management of ocular melanoma from the diagnosis to the treatment.
The eye is an immunologically privileged site; from an evolutionary point of view this condition helps to control or eliminate pathogens while generating the least inflammatory damage to the ocular tissues. However, the counterpart of this phenomenon is that it favors the escape of the tumor cells from the controls of the immune system, facilitating the growth of the uveal melanoma and its metastatic dissemination. Experiments in mice it have shown that cytotoxic cell activity in the ocular tissue might be modulated by two mechanisms:
By direct interference of the specific effector function of CD8 + lymphocytes.
Indirectly affected by stimulation of macrophages .
The inhibition of macrophage action was expressed by an insufficient production of nitric oxide in the ocular tissue, which is known to modulate the tumoricidal activity of cytotoxic T lymphocytes . It has also been shown that tumor rejection in mice by the CD8 + CTL is mediated by TNF-α .
The study of the mechanisms that facilitate the growth of an immunogenic tumor in the anterior chamber, demonstrated an influx of CD8 + CTL infiltrating the tumor. This phenomenon was preceded by intratumoral accumulation of CD11 + myeloid cells B, which exert a powerful immunosuppressive activity on the CTL, facilitating tumor escape from the immune system. Regulatory T cells, myeloid suppressor cells and stroma cells could also reduce the delayed type hypersensitivity reaction to induce apoptosis of CD8 + and NK cells 
4. Demographic and epidemiological data
In a study involving 4500 patients with uveal melanoma, only 0.6% of the cases had a family history of this disease . Thus
5. Diagnosis of ocular melanoma
Accuracy in the early diagnosis of ocular melanoma is crucial to improve the prognosis. Currently the diagnosis of ocular melanoma is based on both the clinical experience of the specialists and on the use of modern diagnostic techniques.
The most common symptoms include
The lesions most commonly mistaken for choroidal melanoma are choroidal nevus (49%), periphereal exudative hemorrhagic chorioretinopathy (8%), congenital hypertrophy of the retinal pigment epithelium (6%), hemorrhagic detachment of the retina or pigment epithelium (5%), circumscribed choroidal hemangioma (8%) and age related macular degeneration (4%) 
5.2. Complementary studies
Other studies like Optical Coherence Tomography and Indocyanine Green angiography may be useful in the diagnosis of this pathology. Magnetic resonance imaging, nuclear magnetic resonance spectography, color Doppler ultrasonography, electrophysiologic testing and inmmunologic testing do not offer reliable results. [20-26]
6. Current medical management of patients with Ocular Melanoma
Cytogenetic: Personalized Targeted Therapy
Currently much effort is directed toward understanding uveal melanoma genetics and genomics , hoping that this knowledge will contribute to the development of effective molecular therapies
Inhibitors of B.Raf and MEK kinases hold promise for treatment of cutaneous melanomas harboring BRAF mutations. BRAF are rare in ocular melanomas, but somatic mutations in the G protein alfa subunits G alfa q and G alfa 11 (encoded by
A randomized phase II study compared MEK inhibition (AZD6244) to temozomide in advanced uveal melanoma. MEK inhibition seems to be a rationale therapeutic strategy in uveal melanoma, using Gnaq/11 as a potential predictor of sensitivity .
Enucleation is indicated when the tumor size exceeds 16 mm of base and 10 mm of height, is diffuse and with bad prognosis; however it is very important to emphasize that there is no scientific evidence of increased survival after enucleation. Also, enucleation does not prevent metastases.
A novel minimally invasive surgical technique for resection of selected cases of small iris tumours has been described. This technique avoids the potential morbidity associated with a large corneoescleral incision allowing for rapid visual recovery .
The most commonly employed form of radiotherapy has been the application of an epiescleral radioactive plaque and the most frequently employed isotopes include
It is extremely important to highlight the conservative treatment of melanoma, proposed by
In a study comparing patients treated with
It has been suggested that length of remaining life after diagnosis of uveal melanoma is similar following enucleation (removal of the eye) to local eye-conserving radiotherapy. The multidisciplinary COMS Group emphasized that there were no differences in survival outcomes and a small difference in quality-of-life outcomes between patients in the brachytherapy arm and those in the enucleation arm .
Radiation treatment was found to reduce the tumor in 94% of the cases. Mean tumor thickness decreased from 3.7 to 2.5 and 2.1 after 3 and 5 years respectively. Recurrence occured in 6% of the treated patients. Although this therapy is associated with complications like radiation optic neuropathy in 81% and vitreous bleeding in 30% of cases, it is a promising treatment given that enucleation was necessary in only 3% of patients and metastasis developed in 15% during follow up. Even though the visual acuity decreases considerably after optic disc irradiation with proton beam therapy, the rates of tumor control and eye retention are favourable.
The second most frequent method of radiotherapy is the use of heavy ions such as
Proton beam irradiation of uveal melanoma has great advantages over brachytherapy because of the homogenous dose delivered to the tumor and the possibility of sparing normal tissue close to the tumor. Complications such as retinal detachment, maculopathy, papillopathy, cataract, glaucoma, vitreous hemorrhage and dryness are described. The severest complication that usually leads to secondary enucleation is neovascular glaucoma and it is encountered after irradiation of large to extra-large tumors. It is hypothesized that the residual tumor scar may produce proinflammatory cytokines and Vascular endothelial growth factor- VEGF (toxic tumor syndrome) leading to intraocular inflammation and neovascular glaucoma. Additional treatments after proton beam such as transpupillary thermotherapy, endoresection of the tumor scar or intravitreal injections of anti-VEGF may reduce the rate of these complications .
Current systemic treatments for metastatic uveal melanoma have not improved overall survival. The fully human anti-cytotoxic T-lymphocyte antigen-4 (CTLA-4) monoclonal antibody,
Transpupillary Thermotherapy (TTT):
Choroidal melanomas should be diagnosed and treated at the very early stage as the initial spread of metastases is thought to occur during the proliferative stage of tumor development.
TTT is recomended for the management of posterior choroidal nevi suspected for malignant transformation or small choroidal melanomas that are less than 2-5 mm in thickness . TTT might be the treatment of choice for selected, very small melanomas. However, studies with long follow-up and large number of patients are needed to evaluate its effectiveness.
Choroidal melanomas treated with TTT as stand-alone procedure need a close monitoring since these tumors developed a significant rate of local recurrences and ocular side-effects in the long run.
Antiangiogenic drugs (Bevacizumab)
Anti-angiogenic therapy is based on the assumption that a tumor cannot grow beyond the limits of diffusion (about 1-2 mm) of oxygen and nutrients from capillaries, unless angiogenesis takes place. VEGF plays a key role in angiogenesis, regulating vasopermeability and the proliferation and migration of endothelial cells. VEGF levels are significantly elevated in uveal melanoma patients with metastatic disease compared to patients without metastases. Anti-angiogenic therapy, such as bevacizumab, is currently used for the treatment of metastases of several malignancies. .
Bevacizumab may be used as an adjuvant agent when used following plaque brachytherapy in the treatment of choroidal melanoma. The combination of this treatments was assessed in an interventional case series of 100 patients treated from 2006-2008 for choroidal melanoma and the results were satisfactory. Melanoma specific mortality was 0% at 9 months after treatment. Mean visual acuity for combined treatment at 6 months was 20/30 
There is no current evidence that chemotherapy has a significant role in the primary management of uveal melanoma. Such treatment may prolong survival for a few months but it is unlikely that it will be curative.
Uveal melanoma metastases develop in 6.5-35% of patients, most commonly to the liver. Metastatic uveal melanoma survival is poor, with 5-7 months of median survival. A retrospective study including 58 patients with uveal melanoma metastases showed that the median overall survival (OS) for all the patients was 10.83 months. Patients who had undergone chemotherapy presented 10.83 months of median OS whereas the patients who did not undergo this treatment had an OS of 8.033 months. Patients with metastatic uveal melanoma should be included in clinical trials evaluating other options with newer agents .
Others (Adjuvant therapy with interferon, Imatinilo Mesylate, Paclitaxeldocosahexaenoico Acid, Factionated Radiosurgery Cyberknife, aflibercept, vaccine).
7. Medical prognosis: Mortality (Hepatic metastasis), loss of the eye, loss of vision
These three variables will affect directly the patient survival:
Class I (60% of the cases)
Low metastatic risk :
IA (87%) almost without metastasis (0.8% of the patients).
IB (13%) few metastasis (10.8%) + disomy cr3 few metastasis.
Class II (40% of the cases)
This classification might be helpful for the prognosis in three aspects: in the screening targeted to metastasis, in the earlier diagnosis of the metastasis and for an earlier preventing treatment of the metastasis in high risk cases. In this regard, it is important to highlight that there is no scientific evidence about increased survival due to metastasis treatment .
8. Uveal melanoma TNM staging and survival: Implications in patient management and prognosis
Large ultrasound diameter
Cilliary body involvement
Years since treatment
Closed PAS+ ve loops
Regional Lymph nodes
First scan (years)
Threshold for next scan (number)
8.2. TNM Stage
The uveal melanoma, which arises from melanocytes residing in the stroma, is the most common primary intraocular tumour in adults. More than 90% involve the choroid, the remainder being confined to the ciliary body and iris.
The most common symptoms in uveal melanoma include visual loss, photopsias and visual field defects but none of these symptoms are specific of this malignancy. Diagnosis is based on slit-lamp biomicroscopy and/or ophthalmoscopy, with ultrasonography, autofluorescence photography. Although each day we count with more variety and helpful complementary studies, suspicious lesions should be closely monitored. Uveal melanomas are diverse in their clinical features and behaviour. Despite ocular treatment almost 50% of patients with primary uveal melanoma will develop distance metastasis . The metastatic disease occurs almost exclusively in patients whose tumour show chromosome 3 loss and/or class 2 gene expression profile. When the tumour shows such lethal genetic changes, the survival time depends on the anatomical stage and the histological grade of the malignancy.
Screening for metastases is more sensitive as a consequence of the advances in liver scanning with magnetic resonance imaging and other methods. More patients with metastases are living longer, benefiting from therapies such as: partial hepatectomy; radiofrequency ablation; ipilumumab immunotherapy; selective internal radiotherapy; intra-hepatic chemotherapy, possibly with isolated liver perfusion; and systemic chemotherapy .
On the basis of the currently available information it appears that patients treated with radiotherapy have a survival rate at least as good, if not better than those treated with enucleation .
Several drugs, such as bortezomib, celecoxib, dacarbazine, anti-angiogenic agents (such as bevacizumab, sorafenib and sunitinib), temsirolimus, mitogen-activated protein kinase kinase (MEK) inhibitors, ipilimumab and AEB071 are candidate drugs, and studies are underway to determine the therapeutic effects of these drugs in uveal melanoma .
Currently, the aim is to improve the detection of uveal melanoma so as to maximize the opportunities for conserving the eye and vision, as well as preventing metastatic spread. Patient management has been enhanced by the formation of multidisciplinary teams in specialized ocular oncology centers all over the world.
This publication was supported by grants from Raymos S.A.C.I. laboratory.