Overview of ISTFs. HA = hyaluronic acid, CHA = Calcium hydroxylapatite, BDDE = butanediol diglycidyl ether, NASHA = nonanimal stabilized hyaluronic acid, PLL = Poly-L-lactic acid, PMM = polymethylmetacrylate
While odontogenic infections are daily encountered in dental and oral and maxillofacial surgery practices, some practitioners may be unfamiliar with the wide range of other infections of diverse etiology, some of them relatively uncommon, or even rare. Patients so affected come to their attention either through referrals from primary care providers or due to patients’ uncertainty about where to seek help for diseases manifesting themselves in the orofacial area. Also in hospital environment, where majority of oral and maxillofacial surgeons practice, one regularly receives requests for consultations about patients who need interdisciplinary cooperation despite the fact that their conditions primarily belong to the sphere of specializations like ENT surgery, ophthalmology, dermatology and others. The purpose of this chapter is to provide an update on such conditions and demonstrate the ways oral and maxillofacial surgeon can participate in their diagnosis and management.
2. Facial skin infections
Impetigo is a highly contagious infection of the superficial epidermis. It is usually caused by
2.1.1. Clinical presentation
The most frequent locations of impetigo are the face and extremities. Two clinical forms are recognized: non-bullous and bullous. The lesions of non-bullous impetigo begin as papules that rapidly evolve into vesicles surrounded by an area of erythema. Then they become pustules that gradually enlarge and break down over a period of 4–6 days to form characteristic golden yellow crusts . (Figure 1)
Bullous lesions appear initially as superficial vesicles that rapidly enlarge to form flaccid bullae filled with clear yellow fluid, which later becomes darker, more turbid, and sometimes purulent. The bullae may rupture, often leaving a thin brown crust resembling lacquer . The lesions heal slowly and leave depigmented areas. Bullous impetigo is caused by strains of
The therapeutic approach to impetigo depends on the number of lesions, their extent and location (in a face proximity to eyelids or mouth), and the need to limit spread of infection to other individuals. The best topical agent is mupirocin, although resistance has been described; other agents, such as bacitracin and neomycin, are considerably less effective. Topical therapy with mupirocin is equivalent to oral systemic antimicrobials and may be used when lesions are limited in number. Patients who have numerous lesions or who are not responding to topical agents should receive oral antibiotics effective against both
Folliculitis is defined as purulent infection of hair follicles limited to the epidermis. Predisposing factors are hot and humid conditions, obesity, diabetes mellitus, long-term antibiotic or corticosteroid use, and immunosuppression .
2.2.1. Clinical presentation and etiology
Folliculitis is characterized by clusters of small, erythematous papules or pustules, usually in body areas prone to friction and heavy perspiration. The face belongs to the most often involved areas. The most common form of folliculitis is
The fungal counterpart of sycosis barbae is
Uncomplicated superficial folliculitis may respond to improved hygiene supported by use of antibacterial soap. If this simple measure is not sufficient, topical antibiotic cream can be used. Refractory or deep infections may require administration of systemic antibiotics. Selection of appropriate antibiotic is based on knowledge of the common microorganisms involved in the particular type of infection before results of microbiology examination and antibiotic sensitivity tests are available. Herpetic folliculitis responds to oral antivirals (e.g. valaciclovir). Eosinophilic folliculitis may respond to isotretinoin, metronidazole, UV-B phototherapy, indometacin or itraconazole . Infectious folliculitis may progress to involve deeper layer of the dermis and finally spread to subcutaneous tissue.
2.3. Furuncle and carbuncle
Furuncle is purulent infection involving the hair follicle and extending to surrounding subcutaneous tissue. (Figure 3)
Furuncles can occur anywhere on hairy skin. A carbuncle is the coalescence of several furuncles with pus draining from multiple follicular orifices. Carbuncles frequently develop on the nape and are more likely to be seen in diabetic patients . In immunocompetent individuals, furuncles and carbuncles are usually caused by
2.3.1. Clinical presentation
In the face, furuncles are frequently seen on the chin, upper lip and paranasal area. Each lesion consists of an inflammatory nodule and an overlying pustule through which hair emerges. Furuncles of the nasal vestibule can be insidious and not obvious upon cursory examination and their symptoms, namely swelling of upper lip and infiltrate of upper oral vestibule, can lead to false impression of odontogenic infection. In patients affected by a facial furuncle, fever and malaise are common. Lesions are extremely painful and they are surrounded by area of cellulitis and collateral edema. (Figure 5)
Small furuncles may burst and heal spontaneously . Application of moist hot dressing can promote drainage. Also gentle removal of overlying crust and necrotic central plug can be helpful; however attempts to express purulent content should be discouraged. Conservative management is preferable and only rarely cases of furuncles or carbuncles progressing into subcutaneous abscess require incision and drainage. In the face, whenever possible, this should be done through intraoral route to avoid facial scarring. Systemic antibiotics are necessary in instances of substantial collateral cellulitis, alteration of general condition and signs of developing facial thrombophlebitis. This initial empirical therapy should be aimed at supposed staphylococcal etiology. Until recently, staphylococcal infections acquired outside of the healthcare setting have been frequently methicillin-sensitive and responsive to a wide range of antibiotics. Since 1980, methicillin-resistent staphylococcus aureus (MRSA) infections have been reported in community outbreaks. These organisms have been called community-acquired or community-associated MRSA, as opposed to hospital acquired MRSA . Hospital acquired MRSA is usually resistant to at least three β-lactam antibiotics and is usually susceptible only to vancomycin, sulfamethoxazole, and nitrofurantoin. Community acquired MRSA is more likely to be susceptible to clindamycin and has varying susceptibility to tetracycline, fluoroquinolone, erythromycin and vancomycin .Outbreaks of furunculosis may occur in families and other groups involved in close personal contact, like prisoners, members of sports teams or outdoor recreation groups [3,11]. Inadequate personal hygiene and exposure to others with furuncles play important role. Control of outbreaks may require bathing with antibacterial soaps, thorough laundering of clothing, towels, bed spreads, separate use of towels and washcloths. Eradication of staphylococcal carriage among colonized persons should be attempted. The prevalence of nasal staphylococcal colonization in the general population is 20–40%, but not all carriers develop recurrent skin infections. Eradication of nasal colonization can be achieved by application of mupirocin ointment twice daily in the anterior nares for the first 5 days each month .
Cellulitis is diffusely spreading soft tissue infection not associated with underlying suppurative foci. It involves rapidly spreading areas of edema, erythema, and may be accompanied by lymphangitis and regional lymphadenitis .
2.4.1. Clinical presentation
In orofacial areas cellulitis is routinely seen as an early stage of odontogenic infections and it is present also at the periphery of other defined skin infections and infected traumatic wounds (Figure 6).
It can also occur as disease per se when organisms enter through breaches in the skin. The breaks in the skin can be small and clinically inconspicuous. Predisposing factors for these infections include conditions that make the skin more fragile or local host defenses less effective, such as obesity, previous cutaneous cuts, venous insufficiency, lymphatic obstruction or other causes .Cellulitis of non-odontogenic origin is most commonly caused by β-hemolytic streptococci (usually group A) but may also be caused by other streptococcal species. Less frequently,
Antibiotic treatment alone is effective in most patients with simple cellulitis. Therapy should include an antibiotic active against streptococci. A large percentage of patients can receive oral medications. Suitable agents include dicloxacillin, cephalexin, clindamycin, or erythromycin. Parenteral therapy is indicated for severely ill patients or for those unable to tolerate oral medications. Reasonable choices include a penicillinase-resistant penicillin such as nafcillin, a first-generation cephalosporin such as cefazolin, or clindamycin or vancomycin for patients with penicillin allergies . In cases of uncomplicated cellulitis, 5 days of antibiotic treatment is as effective as a 10-day course .
Erysipelas is a well-demarcated, painful skin infection characterized by intense erythema. It is almost always caused by β-hemolytic streptococci. The term erysipelas is often used inconsistently and some physicians use it to describe simple cellulitis. The distinction between these two terms relates to the depth of inflammation; erysipelas affects the upper dermis, including the superficial lymphatics, whereas cellulitis involves the deeper dermis and subcutaneous fat. In practice however, distinguishing between cellulitis and erysipelas clinically may be difficult .
2.5.1. Clinical presentation
Erysipelas is distinguished clinically from other forms of cutaneous infection by the following two features: The lesions are raised above the level of the surrounding skin, and there is a clear line of demarcation between involved and uninvolved tissue . (Figure 7)
The skin surface may resemble an orange peel because superficial cutaneous edema surrounds the hair follicles, which causes dimpling. Vesicles, bullae, and cutaneous hemorrhage in the form of petechiae or ecchymoses may develop. Systemic manifestations like fever, tachycardia, hypotension, and leukocytosis may occur, even before the skin abnormalities appear. In older textbooks, pictures of erysipelas of the face characteristically involved the butterfly area, which is nowadays rarely seen.
The first-line treatment of erysipelas is intravenous benzyl-penicillin. In penicillin allergic patients, clindamycin may be used. Anti-staphylococcal drugs are considered if patients fail to improve or have features suggestive of staphylococcal infection like bullous eruptions .
2.6. Craniofacial necrotizing fasciitis
Necrotizing fasciitis (NF) is rapidly progressing bacterial infection spreading along the deep fascial planes with relative sparing of skin and underlying muscles . Necrotizing infection may involve any combination of dermis, subcutaneous tissue, fascia or muscle. Blood supply to the fascia is typically more tenuous than that of muscle or healthy skin, making the fascia more vulnerable to infectious processes. Additionally, the propensity for fluid collection between involved fascia and adjacent tissues further weakens fascial immune protection . The incidence of NF increases with age and most adult cases occur in patients with underlying chronic illness like diabetes, alcohol/drug abuse, immunosuppression, malignancy or chronic systemic diseases. Most patients with NF have polymicrobial infections with an average of 4.4 organisms isolated per infection [17,18]. Although these polymicrobial infections can spread widely and become life-threatening, they tend to be less aggressive than infections caused by a limited number of highly virulent pathogens. These may cause very rapidly spreading necrotizing infections in an immunologically intact host through production of exotoxins. Such pathogens most commonly include
2.6.1. Clinical presentation
Craniofacial NF predominantly originates from periorbital regions;d only one microorganism is usually identified from cultures, most commonly group A hemolytic streptococci. Initial symptoms may resemble simple cellulitis or erysipelas. The distinguishing features are fast progression, pain disproportionate to clinical findings, systemic toxicity and presence of gas. Gas is best detected by CT scan. Fully developed specific clinical picture includes edema that extends beyond skin erythema, cutaneous anesthesia, skin ecchymosis that precedes skin necrosis and presence of bullae .
The promptness of initial surgical debridement is considered decisive for favorable outcome [19,20]. Patients treated surgically on the day of admission have distinctively better prognosis. The early incision and debridement of all involved spaces can salvage the skin, which later in the progress of disease succumbs to necrosis due to thrombosis of feeding vessels. All necrotic tissues should be excised, the defects should be kept open and debridement should be repeated until a completely healthy granulating wound is obtained. While the surgical treatment should be performed promptly, it cannot be as aggressive as in the extremities and trunk, where large areas of skin and subcutaneous tissue are often sacrificed. It is necessary to preserve as much of the anatomic structures as possible to avoid significant cosmetic disfigurement and functional limitations. Simultaneous immediate antibiotic therapy should consist of high-dose penicillin G or ceftriaxone in addition to metronidazole and clindamycin for anaerobic coverage. Clindamycin is a potent suppressor of bacterial toxin synthesis, facilitates phagocytosis of
3. Infected tissue fillers
Injectable soft tissue fillers (ISTFs) are widely popular in facial rejuvenation. ISTFs are usually injected into the deep dermis or dermal – subdermal junction for wrinkles, skin creases or depressed scars . Recently there is a tendency to more frequent use of fillers injected into deep subcutaneous layers for augmentation . ISTFs are effective in treating volume loss and soft tissue redistribution .
3.1. Tissue fillers
All ISTFs with exception of autologous fat are foreign alloplasts. Host tissue response to their presence depends on material type . They can be differentiated as volumetric and structural, or fibroplastic, based on the biomechanics of filling effect . Another practically important property is their time of tissue survival differentiating them into temporary, long lasting or semi-permanent and permanent (Table 1).
The most commonly used ISTFs are homogenous polymer gels, both degradable and nondegradable. They are volumetric; the filling effect stems from the gel itself. Common representatives of
|Collagen||Zyderm||Highly purified bovine dermal collagen in a phosphate-buffered physiological saline containing 0.3% lidocaine||2-4 months|
|Zyplast||Highly purified bovine dermal collagen cross-linked with glutaraldehyde in a phosphate-buffered physiological saline containing 0.3% lidocaine||3-6 months|
|Cosmoderm||Highly purified human-based collagen in a phosphate-buffered physiological saline containing 0.3% lidocaine||3-6 months|
|Cosmoplast||Highly purified human-based collagen cross-linked with glutaraldehyde in a phosphate-buffered physiological saline containing 0.3% lidocaine||3-4 months|
|HA derivatives||Hylaform||HA of rooster combs, 500µ particles, 20 % cross-linked||3-6 months|
|Restylane||HA from S. equi, cross-linked with BDDE; NASHA; 400 μ gel particles, 1% cross-linked||6 months|
|Perlane||HA from S. equi, cross-linked with BDDE; NASHA; 940-1090 μ gel particles||6-12 month|
|Juvederm||HA from ||3-6 months|
|Prevelle Silk||HA from ||2-3 months|
|CHA derivatives||Radiesse||CHA microspheres 25-45 μ in a gel of water, glycerin, and sodium carboxymethylcellulose||1-2 years|
|PLL derivatives||Sculptra||Poly-L-lactic acid mixed with mannitol and sodium carboxymethylcellulose||1-2 years|
|PMM derivatives||Artefill||PMM microspheres 30-50 μ in water-based gel of 3.5% bovine collagen, 0.3% lidocaine, phosphate buffer, and 0.9% NaC||Permanent|
|Silicone||Silikon 1000||Purified polydimethylsiloxane||Permanent|
|Hydrogels||Aquamid||97.5% apyrogenic water and 2.5% polyacrylamide||Permanent|
|Bio-Alcamid||96% apyrogenic water and 4% polyalkylimide||Permanent|
|Autogenous fat||-||Hand –held syringe aspirate, usually from hips or abdomen, sedimented or centrifuged||Semi-permanent|
3.2. Complications of tissue fillers
Complications can be attributed to the product properties, method of delivery and reaction of the recipient's immune system. It is convenient to divide complications according to the time of onset.
3.3. Role of biofilms
Delayed complications of ISTFs have been attributed to biofilms . Biofilms are defined as a structured community of microorganisms encapsulated within a self-developed polymeric matrix and irreversibly adherent to a living or inert surface . They are also often characterized by structural heterogeneity, genetic diversity and complex community interactions. They respond to stimuli, grow and maintain a homeostatic environment. Extracellular polymeric matrix of biofilms may interfere with macrophage phagocytosis and allow for easier exchange of extrachromosomal DNA plasmids encoding antimicrobial resistance. All surgical implants like orthopedic appliances, heart valves, indwelling catheters, stents or other forms of foreign material may be compromised by biofilms. Active clinical infections can flare up weeks, months and even years after initial surgery. Bacteriemia caused by dental treatment, contaminated surgery, or trauma can activate infective response of a chronic biofilm. Once the biofilm has been activated, it leads to acute purulent infection. The active infection can be controlled with antibiotic therapy, but the underlying biofilm can persist and generate a recurrence [27,37,38]. The biofilm theory remains a popular explanation for late infectious complications of ISTFs; but it has been recently challenged and requires further proof .
3.4. Clinical presentation and diagnosis
Acute purulent inflammation caused by infected facial ISTF closely resembles acute odontogenic infection: it causes painful facial swelling, redness, extensive collateral edema and palpable in-depth fluctuation. Deep buccal space and periorbital region are most frequently involved. The general condition is usually altered by fever, malaise and pain. Laboratory sings of acute bacterial infection are present. However, intraoral clinical and x-ray examination fails to discover an odontogenic source of infection, and even if possible odontogenic infectious focus is identified, typical signs of acute odontogenic infection, such as oral vestibule swelling and redness, tooth mobility, or sensitivity to axial percussion are missing . (Figure 8)
Many patients fail to report filler injections on initial interview because they do not consider them as medical procedures or are embarrassed[41,42]. Patients with acute facial infections of uncertain origin should therefore be specifically questioned about a history of cosmetic procedures. Despite of it, some patients will admit application of ISTF only later, when they are confronted with finding of foreign material in a drained exudate.Ultrasound (US) examination can be helpful in establishing the presence of ISTF and its precise location [41,42]. CT imaging may be indicated if there is a suspicion of infection spread, especially orbital cellulitis.
Treatment should follow established principles of dealing with acute purulent infection i.e. eliminate source of infection, drain involved anatomical spaces and provide antibiotic and supportive therapy. When ISTF becomes infected, antibiotic treatment can only mitigate the process and sooner or later after discontinuation of medication recurrence is inevitable. It is therefore necessary to remove all infected material, which is usually identical with drainage of involved spaces. Only small amounts of ISTFs can be removed by aspiration , thus in cases of deep abscesses incision and drainage is the treatment of choice. To avoid facial scarring, intraoral incision is the preferred route. More than one deposit of filler material can be present in any treated area and while one focus is drained another one can remain dormant and consequently undetected on clinical examination. This can lead to recurrence . Characteristic histopathologic findings allow the identification of the specific filler agent. This can be important especially in litigation cases where a number of different fillers have been injected in the same site over the time, or where patients had not been correctly informed about fillers and potential risks .
4. Cervico-facial lymphadenitis
A disease process involving lymph nodes (LNs) is referred to as lymphadenopathy. Lymphadenopathies have multiple etiologies, the most common of which are infection, neoplasia and autoimmune diseases. Inflammation of LN is known as lymphadenitis. The lymphatic system of the cervicofacial region serves as the initial line of defense against infections of all structures within the head, neck, and upper respiratory tract .
4.1. Anatomy and pathophysiology
Diagnosis of the lymphatic infections must be based on the knowledge of anatomic location of LNs, the area and the pattern of lymphatic drainage, and their defense mechanism [45,46]. The lymphatic system of the head and neck contains about 300 nodes, and the extranodal lymphatics of the palatine, pharyngeal and lingual tonsils are known as the lymphatic ring of Waldayer. All the lymphatics from the head and neck drain into the deep cervical LNs . Superficial nodal enlargement usually reflects invasion through an epithelial surface (e.g. skin, oral mucosa), whereas deep nodal enlargement results from an infectious process involving more central structures (e.g. middle ear, posterior pharynx). Lymph nodes contain T- and B-lymphocytes as well as antigen-presenting macrophages (dendritic cells). Tissue lymph enters the LN via one or more afferent vessels and percolates through a series of reticuloendothelial-lined channels that coalesce and drain through an efferent lymphatic vessel.  Once infection occurs, a series of LN reactions follow according to the type and nature of the infectious agent. These will result into signs and symptoms with presentation, which can be acute, subacute, or chronic and can be localized or generalized. Infection of the LNs of the orofacial region can be bacterial, viral, protozoal or fungal. The most common pathogens causing lymphadenitis in the orofacial region include bacterial pathogens as
4.2. Acute bacterial lymphadenitis
Most cases of acute bacterial lymphadenitis occurs in children aged 1 to 4 years. Forty percent to 80% of cases in this age group are due to
4.2.1. Clinical presentation and diagnosis
Patients typically present with concomitant pharyngitis, tonsillitis, acute otitis media, or impetigo. Acute cervical lymphadenitis can also occur following animal bites or scratches. However, there may be a time gap between initial infection at the site of entry and lymphadenitis. LNs enlargement is mostly unilateral, associated with systemic manifestations, such as fever, and malaise. Infected LNs tend to be quite tender with collateral cellulitis and edema. Erythema and increased temperature of the overlying skin are signs of impending liquefaction. Diagnosis is usually based on the clinical picture. Laboratory tests are nonspecific and seldom required. In contrast, laboratory evaluation plays a crucial role in determining the etiology of subacute, chronic, and generalized lymphadenopathy.
Because staphylococci and streptococci are the most common pathogens, initial therapy usually includes a β-lactamase resistant antibiotic; this agent is used because of the high incidence of penicillin resistance in isolated staphylococci. Other treatment options include cephalexin, oxacillin, or clindamycin. Very young patients or patients with severe symptoms may require hospitalization for initiation of parenteral antibiotic therapy and close observation. For older patients with dental or periodontal disease, the antibiotic regimen should include coverage for anaerobic oral flora (i.e., penicillin V or clindamycin). Reports from multiple centers have documented an increasing frequency of community-acquired methicillin-resistant
Drainage should be maintained by insertion of drains (e.g. Penrose or corrugated rubber drain), left in place for 2-3 days. Dressings are changed whenever it becomes saturated by exudate. Antibiotic therapy can be discontinued as soon as clinical improvement is obvious.
4.3. Cat scratch disease
Cat scratch disease (CSD) follows inoculation of
4.3.1. Clinical presentation and diagnosis
CSD presents as regional lymphadenitis associated with a characteristic skin lesion at the site of inoculation. An erythematous skin papule or pustule typically develops 3-10 days after contact with an infected cat (scratch, bite or lick). The patient may suffer low-grade fever and malaise, anorexia, headache and splenomegaly. Regional lymphadenitis develops 5 days to 2 months later. Often the primary site of involvement has resolved by the time lymphadenopathy is noted. The most common sites of lymphadenopathy are the axilla (52%) and the neck (28%). Patients usually present with a single large tender node. Involved LNs undergo sequential changes of lymphoid hyperplasia, granuloma formation, microabscess development, and in some cases suppuration. The most common atypical presentation of CSD is Parinaud’s oculoglandular syndrome (POS). This occurs in up to 17% of CSD patients due to autoinoculation of the eye by rubbing it with their hands after cat contact. POS is manifested either as conjunctivitis with parotid swelling caused by intraparotid lymphadenitis or as an ocular granuloma. Diagnosis of CSD has traditionally required the presence of 3 of 4 criteria: Contact with a cat resulting in a primary lesion, regional lymphadenopathy in the absence of other causes of lymphadenopathy, a positive skin test, and the presence of characteristic histopathological features. The CSD skin test is performed by intradermal injection of heat-inactivated material obtained from a node of a patient fulfilling the diagnostic criteria of the disease. Because of safety concerns about the use of human-derived reagents and the lack of widespread availability, serologic testing for antibodies to
The disease is usually self-limited. Treatment is mainly supportive, with reassurance, hot moist compresses and analgesics. It may be necessary to aspirate pus or surgically remove an excessively large lymph node. Benefits of antibiotic therapy is doubtful. Azithromycin has been shown to be associated with more rapid resolution of nodal enlargement. Tetracycline or erythromycin therapy may also be helpful. 
Tularemia is a highly contagious disease caused by
4.4.1. Clinical presentation and diagnosis
Tularemia in humans can occur in several forms, depending to a large extent on the route of entry. Many cases of disease caused by lower-virulence strains are undiagnosed. The most common form of the disease is ulcero-glandular tularemia, which usually occurs as a consequence of a bite from an infected arthropod vector. After an incubation period of 3 to 5 days, the patient experiences the sudden onset of flu-like symptoms, especially chills, fever, headache, and generalized aches. An ulcer forms at the site of infection. Bacteria are disseminated from this site via the lymphatic system to regional LNs. The enlargement of these LNs often resembles the classical bubo associated with bubonic plague. During early bacteremic phase of the infectious bacteria may be disseminated also to other tissues such as the spleen, liver, lungs, kidneys, intestine, central nervous system, and skeletal muscles. A rare variation of ulcero-glandular disease is oculo-glandular tularemia, where the conjunctiva is the initial site of infection, usually as a result of the transfer of bacteria on the fingertips. The disease is marked by the appearance of ulcers and nodules on the conjunctiva, and without treatment the infection spreads to the regional LNs. The ingestion of infected food or of bacteria in drinking water can result in oropharyngeal tularemia, characterized by sore throat with enlargement of the tonsils and the formation of a yellow-white pseudo membrane, accompanied by swollen cervical LNs. Other, more serious clinical forms of disease are gastrointestinal and pneumonic tularemia. Isolation of bacteria from clinical specimens is possible; however, it needs a special culturing technique. Because of the difficulty in culturing
The drugs of choice for the treatment of tularemia include streptomycin, gentamicin and ciprofloxacin. Ciprofloxacin was the antibiotic with the lowest level of therapeutic failure and with the fewest side effects and was also shown to be suitable for children and in a case where relapse was evident after initial gentamicin therapy .
Syphilis is a sexually transmitted disease caused by infection with
4.5.1. Clinical presentation and diagnosis
Incubation time from exposure to development of primary lesions at the site of inoculation averages 3 weeks but can range from 10-90 days. A papule develops at the site of infection and breaks down to form an ulcer - chancre. The lesion is usually singular, painless, with base infiltration and hardened high margins. After the appearance of the chancre, regional lymphadenopathy occurs. Secondary syphilis develops about 4-10 weeks after the appearance of the primary lesion. Systemic manifestations include malaise, fever, myalgias, arthralgias, lymphadenopathy, and rash. Widespread mucocutaneous lesions are observed over the entire body and may involve the palms, soles, and oral mucosa. The skin lesions are usually macular, discrete, reddish brown, and 5 mm or smaller in diameter; however, they can be pustular, annular, or scaling. The two principal oral lesions associated with secondary syphilis are mucous patches and maculopapular lesions involving the hard palate and manifesting as flat to slightly raised firm red lesions. Of these, wet mucous patches are the most contagious. Even untreated the patient will eventually lose infectivity and pass into latent stage. Tertiary syphilis develops 4-8 years later with progressive multi-organ involvement. The typical tertiary stage lesion is gumma, which in orofacial regions usually involves the hard palate and tongue. [54,55] Regardless of the stage of disease and location of lesions, histopathologic hallmarks of syphilis include endarteritis and a plasma cell rich infiltrate. However, lesional histopathology is not diagnostic. Definitive diagnostic methods are dark field examination and direct immunoflurescent tests of lesional exudates that detect presence of
Parenteral penicillin G is the drug of choice is for all stages of syphilis. Selection of the appropriate penicillin preparation is important, because
4.6. Infectious mononucleosis
Infectious mononucleosis (IM), a common cause of cervical lymphadenitis, is caused by Epstein-Barr virus (EBV), and is its most frequent clinical manifestation. IM is called also “glandular fever”. EBV is ubiquitous herpes virus associated with nasopharyngeal carcinoma, Burkitt’s lymphoma, Hodgkin’s disease, and other lymphoproliferative disorders in immune-deficient individuals. Young children most likely acquire primary EBV infection from close contact that involves exchange of oral secretions via shared items such as toys, bottles, and utensils. Before the age of 10, primary infection is usually asymptomatic or produces an acute illness that is often not recognized as being due to EBV. In adolescents and young adults, primary EBV infection is acquired chiefly by direct intimate oral contact which allows for salivary exchange, and frequently presents as IM. That is where another colloquial name “kissing disease” comes from. Aside from oral transmission, there are reports about transmission by sexual intercourse, contaminated blood, transplanted hematopoietic cells, solid organs, or by intrauterine transmission. 
4.6.1. Clinical presentation and diagnosis
Infectious mononucleosis most often begins insidiously, with vague malaise, followed several days later by fever, fatigue, sore throat, and swollen posterior cervical lymph nodes. Some patients experience an abrupt influenza-like onset, with fever, chills, and body aches. Hepatitis, documented by abnormal liver function tests, is seen in 80% of cases. A useful clinical clue unique to primary EBV infection is eyelid edema, which gives the patient a slit-eyed appearance and may be accompanied by facial puffiness. Virtually all patients given penicillin derivatives develop a rash. Complications include conjunctivitis, hemophagocytic syndrome, myocarditis, neurologic diseases other than meningoencephalitis, pancreatitis, parotitis, pericarditis, pneumonitis, psychological disorders, and splenic rupture. The diagnosis of infectious mononucleosis cannot be made on clinical grounds alone. The appropriate laboratory tests include detection of the presence of atypical lymphocytes, Paul-Bunnel test, monospot test, and detection of EBV antibodies against the viral capsid.
The treatment is mainly symptomatic during periods of fever and malaise and includes limitation of activities, supplementation fluids, nutrition, antipyretics and analgesics. Corticosteroids are indicated for management of complications, such as impending airway obstruction, autoimmune anemia, and autoimmune thrombocytopenia. A number of antiviral drugs have been also used with varying degree of efficiency. 
Rubella is an acute febrile illness of viral origin characterized by rash and lymphadenopathy that affects children and young adults.
4.7.1. Clinical presentation and diagnosis
Rubella infection begins with low grade fever and swollen, tender lymph nodes, usually in the back of the neck or behind the ears. Morbilliform rash appears on the face and spreads downward to the trunk and extremities. As it spreads down, it usually clears on the face. This rash is often the first sign of illness that a patient or a parent notices. No feature of the rash is pathognomic and it looks like many other viral rashes. Other symptoms of rubella, more common in teens and adults, include headache, loss of appetite, mild conjunctivitis with rhinitis, swollen lymph nodes in other parts of the body, and arthralgia.A clinical diagnosis of rubella may be difficult, because many exanthematic diseases may mimic rubella infection. The laboratory diagnosis of rubella can be made either though serologic testing or by viral culture. The serologic diagnosis consists of demonstrating the presence of rubella-specific IgM antibody in a single serum sample or observation of a significant (>4-fold) rise in rubella-specific IgG antibody titers between the acute and convalescent serum specimens drawn 2-3 weeks apart. The nasopharyngeal or throat swab taken 6 days before and after onset of rash is a good source of rubella virus that can be cultured and identified. 
Rubella is mild self-limited illness and no specific treatment is indicated. Maintenance of good hydration, especially replacement of fluids lost through diarrhea or emesis, is the mainstay of management. Intravenous rehydration may be necessary if dehydration is severe. In children and patients with clinical signs of vitamin A deficiency vitamin A supplementation should be considered. Post exposure prophylaxis should be considered in unvaccinated contacts. 
4.8.1. Clinical presentation and diagnosis
Primary infection in the immunocompetent individual is usually asymptomatic. In approximately 10% of this patient group, a non-specific and self-limiting illness is manifested most typically by isolated cervical or occipital lymphadenopathy lasting for less than four to six weeks. Toxoplasmic lymphadenitis most frequently involves a solitary lymph node without systemic symptoms or extranodal disease. The lymph nodes are usually discreet, non-tender, and do not suppurate. Toxoplasmosis can also cause localized lymphadenopathy outside the head and neck areas or generalized lymphadenopathy. After the acute phase, almost all patients will remain chronically infected with tissue cysts that are dormant and cause no clinical symptoms. In contrast, toxoplasmosis in patients who are immunocompromised can be a life-threatening infection. In an immune-deficient patient, the infection can become acutely disseminated and result in pneumonitis, chorioretinitis and encephalitis. [62,63]Toxoplasmic lymphadenitis is most often diagnosed by lymph node biopsy and/or serological assays. Pathological features diagnostic of toxoplasmic lymphadenitis include a reactive follicular hyperplasia, irregular clusters of epithelioid histiocytes encroaching on and blurring the margins of the germinal centers, and focal distention of sinuses with monocytoid cells. The presence of these histological abnormalities alone, when typical, can suffice for the diagnosis. However, to increase the diagnostic yield, serological testing (ELISA, PCR, and IFA) is recommended both in patients with the classical histological features and in those patients with atypical histological findings. Fine needle aspiration cytology (FNAC) is rarely useful for the diagnosis, since it allows visualization of only a few isolated cells and does not permit the evaluation of lymph node architecture.
Acute infection can be treated with a combination of pyrimethamine and sulfadiazine or trisulfapyrimidines. Treatment with pyrimethamine, sulfadiazine and folinic acid is usually reserved for patients who are immunocompromised and those patients who are immunocompetent but have severe or persistent symptoms. Duration of treatment varies from 2-4 months depending upon resolution of clinical signs and symptoms. Alternative drugs include spiramycin, clindamycin, trimethoprime-sulfamethoxazole and various other sulfonamide drugs. Spiramycin is recommended for use in pregnancy till delivery. 
5. Orofacial tuberculosis
Tuberculosis (TB) is chronic granulomatous infection caused by
5.1. Clinical presentation and diagnosis
Because of frequent absence of classic symptoms associated with pulmonary disease, such as fever, cough, weight loss, anorexia, and night sweats, diagnosing EPTB can be a clinical challenge . In the neck, according to ENT literature, the posterior triangle nodes, upper jugular and supraclavicular nodes are most commonly involved [68-70]. Maxillofacial literature describes submandibular and submental nodes as the most often involved lymphatic nodes [73,74,78]. This discrepancy obviously reflects referral bias. Most patients present with an isolated discrete node or a collection of matted nodes. Fluctuant mass or draining sinuses are seen in less than 10% of cases [69,78-9] (Figure 10).
The most frequent locations of oral TB are tongue, vestibular buccal mucosa, gingiva, hard and soft palate. Sometimes the initial presentation can be non-healing extraction wound. Lesions of the oral cavity usually present as painful ulcer and thus mimic squamous cell carcinoma. Most TB lesions are located in the anterior portions of the oral cavity such as the buccal mucosa or vestibule area near the corner of the mouth or lower lip; in contrast the usual location of oral squamous cell carcinoma is on the lateral border of the tongue and retromolar area . Underlying bone can also get directly infected but TB osteomyelitis is probably more frequently due to hematogenic spread. The posterior mandible is more commonly involved, especially the ramus of the mandible and the attached musculature. Rich arterial supply of the masseter and medial pterygoid muscles can play important role as the lesions are frequently seen to involve the outer cortical plates, whereas the medullary bone is unaffected . Tuberculosis of TMJ can be a hematogenic infection or develop by progression from TB otitis media [68,77]. Presenting clinical features are pain, trismus, and swelling. Thus, TMJ TB should be considered in the differential diagnosis of patients presenting with pain and stiffness of the joint . Diagnostic process should begin with imaging methods depending on a location of the lesion: US and CT with contrast or MRI for neck lesions, panoramic X-ray and/or CT for facial bone lesions, MRI for evaluation of TMJ. Patients suspected of EPTB should have biopsy with acid fast smear, histopathology and culture of the lesion, chest radiograph, and sputum culture. While active pulmonary TB occurs infrequently in immunocompetent patients with EPTB, HIV seropositive patients with normal chest films can have active pulmonary TB. Mycobacterial sputum cultures should be performed in this group of patients regardless of chest film results . FNAC is a minimally invasive diagnostic tool and has an established role in the diagnosis of EPTB, including oral lesions. It is easily performed and can be easily repeated. The complication rate following FNAC is small compared to surgical biopsy. Cytology smears should show the epithelioid granuloma with or without necrotic material. In patients with equivocal results on FNAC there may be need for open biopsy when the suspicion of TB is high. Granulomas with necrosis, which are more specific for TB, are more common in excisional biopsy specimens compared with FNAC specimens. Patients with lymphatic EPTB show variable response to the tuberculin skin test . The Mantoux test is positive in more than 90% cases of osteoarticular TB. However, a positive test may also indicate a hypersensitivity reaction to tuberculin proteins or a previous exposure rather, than active TB infection . The diagnosis of TB in the absence of a positive culture requires a combination of epidemiologic and histopathologic criteria as well as a trial of antituberculous medication .
Conservative therapy with anti-tuberculous drugs (isoniazid, rifampicin, pyrazinamide, ethambutol and streptomycin) is the mainstay of treatment. In majority of patients the therapy is started based on pathology results while waiting for culture results, which are not available before 3 weeks. Treatment duration is typically 6 months, and this duration has been shown to be as effective as regimens of 9–18 months.  Adjuvant surgical intervention can be necessary in cases of TB lymphadenitis with large, matted lymph nodes or fluctuant, cold abscesses in the neck. However, these nodes often lie adjacent to great vessels and, if due care is not exercised, injury to great vessels or incomplete excision of the nodes may occur. TB of the TMJ may require abscess drainage, sequestrotomy or even condylectomy. TB of the facial bones may require sequestrectomy and/or sauceriation. TB of the salivary gland, oral cavity and ear respond very well to antituberculous therapy and do not require surgical management.
6. Atypical (non-tuberculous) mycobacteriosis
Nontuberculous mycobacteria (NTM) are ubiquitous organisms that typically reside in soil. They are facultative pathogens and their pathogenicity depends on the interaction between the microorganism and the host's immune system. About 90% of NTM infections involve the pulmonary system. Other frequent locations are lymph nodes, skin, soft tissues and bones.  Even less frequent are central nervous system disease, keratitis, and otitis media . The most frequently isolated species is
6.1. Clinical presentation and diagnosis
In orofacial region the most prevalent location of infection by NTM are lymphatic nodes. The disease most commonly affects children with peak incidence at 1-5 years of age [82-4]. The port of entry is probably oropharyngeal mucosa and the lymphatic vessels that drain the mouth and pharynx. Primary infectious focus can also be facial skin . The disease is usually unilateral and affects jugulodigastric, submandibular, parotid/pre-auricular, submental, and posterior triangle lymph nodes. Most patients are otherwise healthy and a chronic neck mass that does not respond to antimicrobial therapy is their sole clinical sign. On average the cervicofacial lymphadenopathy is present for 12 weeks before the proper diagnosis is established and treatment initiated . The size of the infected lymph node can range from 1 to 6 cm and is typically non-tender. The nodes can occasionally liquefy, which is accompanied by fixation of overlying skin, violaceous discoloration, parchment-like transformation of skin and finally formation of draining sinus. In untreated cases, healing usually occurs by unsightly fibrotic scaring and calcification. (Figure 11)
Contrast-enhanced CT imaging picture characteristic of NTM lymphadenitis is asymmetrical lymphadenopathy with contiguous, low density ring-enhancement. Inflammatory changes involving the subcutaneous tissue, such as fat stranding are absent but necrotic foci within skin and subcutaneous tissue are not uncommon . PPD testing has been shown to produce variable results. NTM-specific antigen skin testing can be a useful diagnostic measure, but it is rarely readily available . Diagnosis depends upon the identification of NTM. This requires obtaining material for culture. Tissue samples by FNAC or tissue biopsy are usually necessary, because sampling of draining or ulcerated lesions by swabs do not provide sufficient diagnostic yield. FNAC is the preferred diagnostic technique for patients who do not undergo surgical excision. Histological appearance of necrotizing granulomatous inflammation with various degrees of caseation is also diagnostic. The most important differential diagnosis is TB lymphadenitis. 
Treatment of uncomplicated NTM lymphadenitis is surgical excision [86-7]. Total excision should be performed as early as possible to prevent spread and subsequently more difficult surgery with possible cosmetic consequences. Adjacent normal-appearing enlarged lymph nodes should also be excised. Curettage might be considered as an alternative in cases of adherence of the facial nerve branches. Incision and drainage lead to sinus tract formation with chronic discharge and should be avoided . In a clinical trial including 100 children with culture or polymerase chain reaction confirmed diagnoses, surgery was more effective than chemotherapy with cure rates 96% and 66%, respectively. However, for patients with discharging sinus or proximity of facial nerve branches, chemotherapy can be the preferred therapeutic modality. Chemotherapy must also be considered for patients in whom surgical treatment is unsuccessful. Chemotherapy usually includes clarithromycin and rifabutin. 
7. Salivary gland infections
Salivary glands (SGs) are exocrine, merocrine glands. Major SGs are the parotid, submandibular and sublingual. The minor SGs are distributed through the mucosa of the oral cavity. While both major and minor SGs can become infected, infection usually affects major SGs, especially the parotid gland. Infection of SGs can be bacterial, viral, fungal, or as was recently documented, protozoal.
7.1. Bacterial infections
Bacterial infections of the SGs typically result from retrograde propagation of bacteria through their ducts from oral cavity. This process is promoted by stasis of salivary flow.  Predisposing factors for the ductally ascending infection are dehydration, xerogenic drugs and salivary gland diseases associated with reduced saliva secretion or ductal obstructions. Other possible modes of infection are through transitory bacteremia, especially in the neonatal period, or direct spread from adjacent infectious processes. [89,90]
7.1.1. Acute bacterial sialadenitis
The parotid gland is the most common site of acute suppurative salivary infection. Saliva of the parotid gland is primarily serous and therefore provides less protection against ascending bacteria. On the other hand, mucoid saliva produced by the submandibular and sublingual glands contains many antimicrobial protective elements, including lysozymes and IgA antibodies. Mucins also contain sialic acid, which agglutinates bacteria, preventing its adherence to host tissues. Specific glycoproteins found in mucins bind epithelial cells, competitively inhibiting bacterial attachment to these cells.  Submandibular sialadenitis is less frequent and accounts for approximately 10% of all cases of sialadenitis of the major SGs. Majority of submandibular gland infections is related to sialolithiasis of Wharton’s duct. Submandibular secretions are more mucinous, and therefore more viscid; they also are more alkaline, containing a higher percentage of calcium phosphates. These circumstances contribute to the fact that 85-90% of salivary calculi are located in the submandibular duct.  (Figure 12)
The most common pathogens associated with acute bacterial infections of SGs are
22.214.171.124. Clinical presentation and diagnosis
Local symptoms include a rapid onset of pain, swelling, and induration of the involved gland. Overlying skin can become purplish as infection progresses. Stensen’s or Wharton’s ducts may appear erythematous and gentle massage of the gland will frequently result in a suppurative discharge from the duct orifice. In submandibular infections, a calculus may be palpable along the course of Wharton‘s duct. Because of the resistance of the fibrous capsule, particularly that surrounding the parotid gland, palpation of the abscessed gland may fail to reveal fluctuance. Systemic manifestations like fever, chills, malaise are frequent.  Laboratory examination revels leukocytosis with neutrophilia. Purulent secretions from duct orifice should be sent for microbiology examination. CT or US imaging of the gland may reveal abscess formation; however they are not indicated at the beginning of the disease. In the case of submandibular gland infection, orthopantomogram may disclose a salivary stone. Sialography is contraindicated in the acute phases of sialadenitis because it is extremely painful and can exacerbate the existing inflammation. 
Elimination of the etiological factor such as ductal obstruction by sialolith is essential. Other therapeutic measures include proper hydration, stimulation of saliva flow, analgesics and local heat application to ease the discomfort. Capable patients should be instructed on regular external or bimanual massage, starting from the distal bed of the gland and working in the direction of duct drainage.  Initially broad-spectrum antimicrobial therapy is indicated to cover all possible aerobic and anaerobic pathogens. Clindamycin, cefoxitin, imipenem, the combination of metronidazole and macrolide or penicillin plus a β-lactamase inhibitor, provide adequate coverage. Later the therapy should be guided by results of culture and antibiotic sensitivity. Presence of methicillin-resistant staphylococci may mandate the use of vancomycin or linezolid.  In most cases of acute submandibular sialadenitis removal of duct obstruction and conservative therapy are sufficient to resolve the disease. In cases of acute bacterial parotitis, especially in medically compromised patients like diabetics, infection process often reaches the stage of abscess, despite antibiotic treatment. US, CT or MRI imaging help to recognize this condition. In such instances evacuation of pus becomes necessary. Small, superficially located abscess can be aspirated. The classical approach to drainage of parotid abscess involves anteriorly based facial flap, and multiple, superficial, radial incisions in the parotid fascia parallel to the facial nerve branches . Based on our experience, we consider such radical surgery unnecessary and impractical, and instead utilize incision placed in natural skin crease, as close as possible to the abscess, and dissect bluntly using fine mosquito forceps (Figure 13).
7.1.2. Chronic bacterial sialadenitis
Like in acute sialadenitis, the causative event in chronic sialadenitis is believed to be a lowered secretion rate with subsequent salivary stasis. This can be due to neglected underlying obstruction (duct stenosis, stone or foreign body). Another major cause of chronic sialadenitis is Sjögren’s syndrome. Approximately 2% of patients with Sjögren’s syndrome are affected each year . Repeated acute suppurative infections lead over time to permanent damage characterized by sialectasis, ductal ectasia, and progressive acinar destruction combined with a lymphocytic infiltrate. The structure of parenchyma and function of the gland are gradually destroyed. This leads to decrease in salivary secretion and further promotes recurrences in a vicious circle. Some authors feel that chronic sialadenitis is in most instances either autoimmune or of unknown etiology with superimposed bacterial infections and should not be designated as a chronic bacterial infection .
126.96.36.199. Clinical presentation and diagnosis
Chronic sialadenitis is characterized by recurrent moderate swelling of the affected gland alternating with asymptomatic remissions. During flare-up the duct orifice appears inflamed with accompanying purulent discharge. Symptomatology tends to become progressively more severe with increasing number of flare-ups. Eventually in some patients, the clinical manifestations of a flare-up can mimic that seen in acute sialadenitis including abscess formation . With recurrent infection the gland atrophies and is replaced by fibrotic tissue, which makes it permanently palpable. Many patients with chronic parotitis seek medical attention because of a non-tender asymptomatic parotid lump or diffuse swelling. Chronic sclerosing sialadenitis of submandibular gland, characterized by progressive periductal fibrosis, dilated ducts with a dense lymphocyte infiltration with lymphoid follicle formation and acinar atrophy, is known as Küttner’s tumor. It creates a diagnostic dilemma, because clinically it resembles a submandibular gland tumor and it is the known fact that 80% of tumors presenting in this organ are malignant .Clinical diagnosis of chronic sialadenitis needs validation by imaging. Common diagnostic methods are US and sialography. Sialography can evaluate the possible cause and the location of obstruction and enables assessment of progression; however, it is an invasive method and is currently being supplanted by MRI sialography, which provides 3-dimensional images of the salivary gland without contrast medium or exposure to ionizing radiation . A new effective method for diagnosis and also treatment of the obstructive disorders of SGs is sialoendoscopy [98,99].FNAC or incisional biopsy is advised for lesions that are still not diagnosed after complete clinical and radiographic evaluation.
An appropriate antibiotic should be used during an acute flare-up. Specific treatment is instituted for any structural abnormality, stricture or calculus. Oral and dental hygiene with mouthwashes, massage and compresses are useful. In the majority of cases of chronic parotitis, the disease will subside without an operation. More aggressive treatment is justified only for those patients with persistent problems. Total parotidectomy is advised only with frequent attacks and severe, progressive disability . In a case of chronic submandibular sialadenitis, when the function is destroyed, the treatment is by surgical excision. Interventional sialoendoscopy is an innovative approach to management of chronic sialadenitis. It mainly includes sialolith or ductal polyp removal. When present, sialodochitis can be controlled by continuous lavage and drug perfusion in the duct. It has also been reported that polyethylene stents can be used to prevent obstruction of the duct lumen by postoperative edema, to allow particles of calculus to be washed out by the saliva and to reduce the possibility of stenosis. 
7.1.3. Juvenile recurrent parotitis
This separately recognized suppurative disease of the parotid glands is characterized by recurrent unilateral or bilateral parotid swellings that may persist into adulthood. Age of onset is most commonly between 3 and 6 years, while complete remission is usual at the time of puberty. An early age at the first episode is associated with an increased risk of recurrences. The episodes of parotid swelling may occur several times per year and overall number of recurrences can reach several dozen. [101,102] The etiology and pathogenesis of this condition are largely unknown. Numerous factors were suggested as causative: congenital malformation of the ductal system, hereditary and genetic factors, allergy, autoimmune disease, IgA or IgG3 deficiency [102,103].
188.8.131.52. Clinical presentation and diagnosis
Findings include recurrent episodes of glandular swelling which typically last for several days up to 2 weeks, and which may occur more than 10 times per year, generalized malaise and pain. On clinical examination the affected gland is swollen and tender, mostly without overlying skin changes. Saliva expressed from the duct is thick and contains floccules of inspissated mucus or pus. US is the appropriate initial imaging investigation, and is usually supplemented by sialography after acute symptoms have subsided. (Figure 14)
Acute episodes are managed conservatively with hydration, stimulation of salivation, fomentations and analgesic-antipyretic medication. Patients with fever and frank purulent exudation may require a course of antibiotics. The sialography with iodinated oil may itself cause an improvement of the condition .
7.2. Viral infections
Viral infection of the SGs most commonly occurs through hematogenous dissemination, although infection by retrograde ductal migration does occur. Viral infestation of salivary parenchyma can be accompanied with local and/or systemic manifestations. There is a wide range of viral infections that can involve SGs, which include
Mumps is the most common childhood viral disease, causing nonsuppurative acute sialadenitis. Adults are rarely infected due to life-long immunity incurred by childhood exposure or MMR vaccination. Mumps virus, the causative agent of mumps infection, is an enveloped RNA virus that belongs to the genus
184.108.40.206. Clinical presentation and diagnosis
Mumps is characterized by pain and swelling of one or both parotid glands, accompanied by low-grade fever, arthralgia, malaise, and headache. Bilateral parotid gland swelling occurs in most cases, but submandibular gland swelling can also occur in rare cases. Progression of parotid gland swelling can be rapid and sufficient to cause displacement of the pinna. Pain is usually exacerbated by the physiologic stimulus of eating, which causes contractile ejection of saliva from the inflamed gland. Findings at the orifice of the parotid duct are usually absent but sometimes the orifice can become edematous and erythematous. Ductal epithelial desquamation may lead to secondary ductal obstruction and dilatation. While the parotids are the most commonly affected organs, parotitis is not a primary or necessary step for mumps infection. More fulminant infections occasionally progress to include meningoencephalitis, orchitis, pancreatitis, and nephritis. Routinely obtained laboratory tests are usually unremarkable except for occasional leukopenia. Elevations in serum salivary type iso-amylase parallels the pattern and duration of glandular swelling. Laboratory investigation is rarely required given the characteristic features present in all but exceptional cases. A laboratory diagnosis is based on isolation of the mumps virus, detection of viral nucleic acid, or serological confirmation. Histological examination reveals substantial cytoplasmic vacuolization of acinar cells. [89,92,105]
As with any viral illness, there is no specific antiviral therapy for mumps and treatment is mostly symptomatic and supportive: supplemental hydration and rest, with dietary modifications to minimize glandular secretory activity. Generally, the symptoms of viremia, including fever, arthralgia, malaise, and headache, begin to abate within 3 to 7 days. The resolution of gland swelling usually requires several weeks, frequently proceeding asymmetrically. 
7.3. Fungal infections
Fungal infection is an unusual cause of SG pathology, however there are several reports in the literature about infection of salivary gland with
7.4. Parasite infections
Apart from involvement of the parotid gland by toxoplasmosis , we were able to find only one case of parasitic SG infestation in the English literature. A nematode larva, morphologically consistent with
7.5. Granulomatous infections
Granulomatous SG infections not infrequently represent a manifestation of a chronic granulomatous disease involving the lymphatic network in and around the parotid gland. Also direct infiltration of the adjacent glandular parenchyma occurs in fulminant cases. Manifestations frequently feature asymptomatic gradual enlargement of a nodule within the gland substance, suggesting a neoplasm. Included among these diseases are mycobacterial diseases (tuberculous and atypical forms), actinomycosis, cat scratch disease, and tularemia.  For details see respective sections of this chapter.
8. Paranasal sinuses infections
Sinusitis is one of the most common condtions in primary care. Because infection causes inflammation of both the sinuses and the nasal cavity, the term "rhinosinusitis" instead of the more common sinusitis has been recently coined [112,113]. The precipitating factor in acute sinusitis seems to be blockage of the sinus ostium, typically the maxillary sinus ostium situated under the middle turbinate, with mucus retention and subsequent infection. Viral infection of upper respiratory tract triggers most cases. Only 0.2-2% of cases become complicated by bacterial infection . Worsening symptoms after 5 days or persistent symptoms beyond 10 days (but less than 12 weeks) indicate non-viral rhinosinusitis; whereas viral disease lasts less than 10 days . A small proportion of cases of bacterial sinusitis can arise as a result of periapical infection or untreated post-extraction oro-antral communication. Odontogenic maxillary sinusitis comprises 10–12% of bacterial sinusitis; however, recent studies suggest that this figure is much more frequent and closer to 30%. 
8.1. Clinical presentation and diagnosis
Clinical studies have recently confirmed that about 60% of presumed bacterial sinusitis resolves spontaneously without antibiotics. For instance, in a double-blind, randomized, placebo-controlled factorial trial of 240 adults (aged ≥16 years) with acute non-recurrent bacterial sinusitis, neither an antibiotic nor a topical steroid alone or in combination were effective in altering the symptom severity, the duration, or the natural course of the condition . Despite this evidence, antibiotics are still overused, which ads to treatment costs, puts patients at risk of adverse events and ads to growing antimicrobial resistance .Treatment of
9. Orbital infections
Infections of the orbit make up less than 1% of all orofacial acute bacterial inflammations. Their rarity may lead to late diagnosis and consequential serious complications: impairment of visual acuity, blindness, or in extreme cases even death due to intracranial spread .The orbit is surrounded by frontal bone, major and minor wing of sphenoid bone, orbital facet of vertical plate of palatinal bone, lamina papyracea of ethmoid bone, lacrimal bone, maxilla and zygomatic bone. Several of these bones contain pneumatized paranasal sinuses. The ethmoidal sinus and maxillary sinus are separated from the orbital cavity by very thin bone shell. There are also numerous bony foramina and fissures containing neurovascular bundles, along which the infection processes can spread into orbit and intracranially: optical foramen, superior and inferior orbital fissure, anterior and posterior ethmoidal foramen, infraorbital canal and foramen, nasolacrimal canal, supraorbital and supratrochlear foramen. Another route of orbital involvement by adjacent infectious process is thromboflebitis of orbital veins, which are connected to facial venous system by angular, infraorbital, supraorbital, supratrochlear and pterygoid plexus veins. The ophthalmic veins communicate also with the veins of the sinuses, especially the ethmoid sinus. The ophthalmic veins drain into the cavernous sinus, and therefore infections can spread intracranially. Veins in this region are valveless and can have retrograde flow. This makes venous orbital system prone to congestion.  Orbital infections are usually classified as pre-septal or post-septal according to their relationship to orbital septum, connective tissue membrane, which arises from the orbital margin and radiates into upper and lower tarsus of the eyelids. . Conditions regarded as preseptal orbitocellulitis are quite frequent. They accompany many orofacial and upper respiratory infection processes, especially odontogenic infections originating in the maxilla. Children are regularly affected by this scary-looking condition; however, it recedes readily after the underlying cause has been eliminated. In these cases periorbital soft tissues and eyelids are affected by edema, but ocular findings like globe position, motility and vision remain normal. Only post-septal processes can therefore be considered true orbital infections. (Figure 16)
In postseptal orbital cellulitis there is diffuse edema of the orbital contents and actual infiltration of the adipose tissue with inflammatory cells and bacteria, but no discrete formation of abscess. In subperiosteal orbital abscess, there is a collection of pus between the periosteum and the bony wall of the orbit, while in intraorbital abscess pus collection is present within the orbital tissues. [122,123] (Figure 17)
The close relationship between the orbit and paranasal sinuses is responsible for the majority of orbital infections, especially in children. Paranasal sinusitis, mostly ethmoiditis followed by maxillary sinusitis, precedes 60 – 84% of orbital infections. [124,125] Other sources of orbital infections are trauma, retained foreign bodies, periorbital suppurative skin diseases, hordeolum or chalazion, dacryocystitis and conjunctivitis. Odontogenic infection of the orbit is rare. The pathway can be via the maxillary sinus, the canine fossa with a thrombophlebitis of the angular vein, or the pterygopalatine fossa and infratemporal fossa and further through the inferior orbital fissure.  The causative microorganisms in acute orbital infections are usually those associated also with paranasal sinusitis. Before introduction of vaccination,
9.1. Clinical presentation and diagnosis
Symptoms depend on the stage of the infectious process, which begins with postseptal orbital cellulitis accompanied by swelling and erythema of eyelids, conjunctival chemosis, limited ocular motility, visual disturbances and proptosis due to developing intraconal edema. Development of subperiosteal abscess can lead to displacement of the bulbus with resulting diplopia. Intraconal progress of infection is marked by increasing exophthalmia, abnormal pupillary reflexes, ophthalmoplegia, impaired color vision and decreasing visual acuity. Eyeball is painful to touch and patient suffers from severe headache. The disease is accompanied by septic fever and laboratory signs of acute bacterial infection. Untreated or inadequately treated disease finally progresses into thrombophlebitis of cavernous sinus with complete paralysis of related cranial nerves, loss of vision, altered mental status and generalized sepsis. Mortality rate of cavernous sinus thrombophlebitis remains high. There is usually very little to be gained by conventional radiology examination. Plain radiographs are reserved for very young children in whom the risk of sedation for the CT scan and radiation burden outweigh the possible diagnostic yield . CT scan or MRI imaging should be performed without delay to serve as an indicator and guide for surgical intervention. I it will help to elucidate the status of the paranasal sinuses, where majority of infections originate. Although CT still remains the modality of choice for the diagnostic workup of orbital infection, MRI should be considered particularly in the pediatric population . Recently, there was some progress in employment of US as a readily available, inexpensive imaging method for diagnosing and monitoring orbital infections, especially in children, where radiation dose is a major concern .
Patients with cellulitis, without evidence of a subperiosteal or intraorbital abscess, can usually be treated with parenteral antibiotics alone. The antibiotic should be broad spectrum and to cover aerobes as well as anaerobes. Second- or third-generation cephalosporins, ampicillin-sulbactam, ticarcillin-clavulanate, clindamycin, aminoglycosides, fluoroquinolones or carbapenems are among those recommended. [121,128] Surgical incision and drainage of the subperiosteal or intraorbital abscess is the mainstay of therapy and should be considered an emergency procedure. Primary source of infection should be addressed at the same time. The optimal way of draining orbital abscess is endoscopic access via paranasal sinuses, namely in cases originating in sinusitis . Otherwise, surgical access to the orbit is through periorbital skin incisions like Lynch, infraorbital or lateral eyebrow. Cosmetic approaches used in orbital traumatology are not indicated here, because they do not allow placement of proper drains and/or they would bring purulent exudate into the conjunctival sac and contaminate the cornea. Surgical intervention should be complemented by aggressive intravenous antibiotic therapy without waiting for results of microbiology examination and sensitivity testing. Benefits of corticosteroid therapy aimed at reducing orbital edema is questionable. It should be administered only if mycotic etiology has been ruled out.
10. Interstitial fungal infections
There are about 100,000 different species of fungi worldwide, but only a few are pathogenic for humans, and most of them show distinct geographic distribution. With the exception of candidiasis, other fungal infections are extremely rare and consequently medical and dental practitioners have limited experience and knowledge in their diagnosis and management. Accurate and early diagnosis, which often is not easy, should lead to prompt and aggressive therapy to prevent spread, dissemination and death. Fungal infections rarely afflict healthy immunocompetent individuals. However, recent years saw a dramatic increase in the numbers of immunocompromised patients, above all HIV infected persons, diabetics, patients with hematologic malignancies, transplant recipients and other patients receiving immunosuppressive drugs. Clinicians should be aware of these rare mycoses and include them in the differential diagnosis when dealing with unusual or unexplained symptoms. The laboratory methods available to diagnose fungal infections include biopsy and culture of tissue, body fluids, secretions, tests for antigens and serum antibodies. Biopsy investigation is the key to correct diagnosis and should include special stains such as periodic acid-Schiff and Grocott-Gomori methenamine silver nitrate. For yeasts, culture is necessary to identify the etiologic agents. Filamentous fungi, in particular zygomycetes and dimorphic fungi can be diagnosed by histological examination and pertinent stains with or without isolation of the fungus from the same site. 
Combination of amphotericin-B and flucytosine is the treatment of choice for the first 2 weeks, followed by fluconazole maintenance therapy.
Aspergillosis is an infection caused by a fungus of the
Zygomycosis is fungal infection also known under designations phycomycosis or mucormycosis. The term phycomycosis is obsolete and refers to some of organisms currently classified as
10.4.1. Clinical presentation and diagnosis
Majority of orofacial zygomycosis cases originate in paranasal sinuses, especially in diabetic patients. Initial signs include nasal obstruction, mucopurulent or bloody nasal discharge, nasal crusting, facial pain, headache, facial swelling, and cellulitis. In acutely progressing cases, orbital involvement is a common clinical feature, even on presentation. (Figure 19)
Zygomycosis should be considered in all patients with orbital inflammation associated with multiple cranial nerve palsies and retinal or orbital infarction, regardless of their immunologic status. Initial orbital involvement is an alarming sign, because it can lead to intracranial progression with grave prognosis. CT scan at this initial stage often reveals only minimal mucosal thickening of the sinuses. Blood tests, cerebrospinal fluid examinations, and cultures from paranasal sinuses fluid are of no diagnostic help; only the detection of typical fungal hyphae in the infected tissue is diagnostic. The early collection of biopsy sample is therefore of utmost importance. [146-8] Chronically developing zygomycosis of paranasal sinuses results in extensive necrosis and destruction of mid-facial bony architecture and can manifest itself by hard palate ulceration over necrotic bone and finally palate perforation. (Figure 20)
Effective therapy requires prompt surgical intervention, systemic antifungal drug administration and reversal of the underlying immunocompromising condition. The only agent active against most
Histoplasmosis is a mycosis caused by
11. Deep neck infections
Infections of deep fascial compartments of the head and neck can be challenging in diagnosis and management. Because of the anatomic communication between fascial neck spaces, the infection processes easily spread beyond the original site and can lead to life threatening complications. Most deep neck abscesses are polymicrobial; the average number of isolates is five (range 1–10). Anaerobic bacteria can be isolated from most abscesses when appropriate culture techniques are employed. Predominant anaerobic organisms isolated in peritonsillar, lateral pharyngeal and retropharyngeal abscesses are
11.1. Peritonsillar abscess
The most common deep neck infection is peritonsillar abscess. Peritonsillar abscesses mostly occur as a complication of repeated episodes of bacterial tonsillitis, but they can occasionally occur as a complication of a viral infection, such as Epstein–Barr virus mononucleosis. The infection penetrates into the potential space between the superior constrictor pharyngis muscle and the tonsillar capsule. [90,161]
11.1.1. Clinical presentation and diagnosis
The peritonsillar abscess is usually preceded by acute pharyngotonsillitis. The initial focus of infection may have been resolved by the time of presentation.  Affected tonsil is swollen and inflamed, but the soft palate does not bulge. The uvula is edematous and pushed towards the opposite side of the infection. Patients have difficulty in swallowing or speaking. They may be drooling because of pain on swallowing. Pain gradually increases in severity, radiates to the ear and causes trismus as a result of spasm of the medial pterygoid muscle. The breath has a foul odor. Ipsilateral cervical lymph nodes are enlarged and tender. CT examination, or intra-oral US, is helpful in distinguishing between abscess and cellulitis. 
The therapy of choice is needle aspiration or incision and drainage of the abscess under local or general anesthesia, supported by administration of parenteral antibiotics. Hospitalization and general anesthesia are required in younger children. It is important to obtain adequate specimens for microbial culture from the abscess as a variety of organisms can be recovered. Specimens are best collected at the time of surgical drainage by needle aspiration.  Patients with a peritonsillar abscess and a history of recurrent tonsillitis should be considered for tonsillectomy after the acute episode has subsided. It is also possible to drain abscesses by tonsillectomy during the acute stage of the disease.
11.2. Parapharyngeal abscess
The parapharyngeal space is a crevice extending from the level of hyoid bone to the base of the skull. Its lateral border is made up by the medial pterygoid muscle and part of ascending ramus of the mandible, the deep lobe of parotid gland with its investing fascia and inner surface of sternocleidomastoid muscle with its investing fascia. The medial border is composed of the buccopharyngeal fascia covering the lateral surface of the superior constrictor muscle. The pterygomandibular raphe, formed by the junction of the buccinator and the superior constrictor pharyngis muscles, is the anterior border. In this area parapharyngeal space has intimate relationship to pterygomandibular space. The posterior border is made up by the alar fascia and along it parapharyngeal space communicates with the retropharyngeal space. Superior border is the skull base and inferiorly parapharyngeal space communicates with paravisceral neck space. The styloid process and muscles attached to it, together with surrounding loose connective tissue and stylohyoid ligament, create styloid septum, dividing the parapharyngeal space into prestyloid and retrostyloid compartments. The prestyloid compartment does not contain any important structures except ascendant palatine artery, and is closely adjacent to the tonsillar fossa and the medial pterygoid muscle. The retrostyloid compartment contains internal carotid artery, internal jugular vein, the cranial nerves IX - XII, and the cervical sympathetic trunk, as well as lymphatic nodes. Parapharyngeal space can be infected from various sources including the pharynx, tonsils, parotid gland, submandibular space, retropharyngeal space, masticator space, and local lymph nodes. The most frequent source of parapharyngeal infection is peritonsillar abscess.  Complications arising from infections of the parapharyngeal space are caused predominantly by involvement of the retrostyloid compartment. They include Horner’s syndrome or cranial nerves IX to XII palsies. Involvement of the vagus nerve or laryngeal edema and obstruction can lead to sudden death. Suppurative jugular thrombophlebitis (Lemierre syndrome) is characterized by an anaerobic septic thrombus occluding the internal jugular vein, often with bacteremia and metastatic foci of infection.  Carotid artery erosion and rupture also can occur, with devastating consequences, characteristically preceded by small ‘‘herald bleeds’’.  In addition, infections of the parapharyngeal space can spread to other spaces of the head and neck.
11.2.1. Clinical presentation and diagnosis
The clinical manifestations of the parapharyngeal space infection depend on whether the prestyloid, retrostyloid or both compartments are involved. The clinical symptoms of infection of the prestyloid compartment are dysphagia, trismus, and pain involving the ipsilateral side of the neck with potential projection to the ipsilateral ear. Flexion of the neck intensifies pain by physical compression of the space. On physical examination, swelling and induration may be noticed at the angle of the mandible and parotid area. When an adequate oro-pharyngeal examination is not hampered by trismus, the lateral pharyngeal wall is often found displaced medially. There may be other physical findings associated with the portal of infection, like tonsillitis or pharyngitis. However, often the infection focus does not cause prominent symptoms. An antecedent pharyngitis or tonsillitis may already have resolved. Isolated infections of the retrostyloid compartment of the parapharyngeal space lack the intense trismus associated with prestyloid compartment infections. Occasionally, the parotid gland, which is adjacent to the retrostyloid compartment, may become swollen. Edema may involve the epiglottis and larynx, leading to dyspnea. An oropharyngeal examination may miss swelling of the pharyngeal wall because the swelling can be hidden behind the palatopharyngeal arch. Some of patients thus have no specific localizing signs and may present with sepsis of occult origin. The diagnosis may become apparent only on imaging or after the development of neurologic or vascular complications. Infections of either compartment are associated with systemic toxicity, fevers, chills, and potentially rigors. [90, 161] (Figure 21).
Infections of the parapharyngeal space, especially prestyloid compartment, are usually suppurative and have tendency to spread rapidly. They should be managed by early surgical drainage and antibiotic therapy. Some authors suggest that infections localized to the retrostyloid compartment with no clinical evidence of sepsis or airway compromise may respond to intravenous antibiotics without surgery.  These two forms can be differentiated by contrast CT scans. 
11.3. Retropharyngeal abscess
The retropharyngeal space is surrounded anteriorly by the posterior wall of the pharynx, superiorly by occipital bone and bilaterally by loose connective tissue contiguous with both parapharyngeal spaces. The posterior wall is made up by the alar fascia derived from the deep layer of cervical fascia. Retropharyngeal space extends caudally and communicates with the mediastinum. Retropharyngeal abscesses occur more frequently in children. It is surmised that young children are more prone to retropharyngeal abscesses due to the numerous lymph nodes in the space, while in adolescents and adults retropharyngeal lymphatics are regressed Retropharyngeal abscesses in adult age occur mostly in immunocompromised patients or as a foreign body complication. 
11.3.1. Clinical presentation and diagnosis
Clinical manifestations of retropharyngeal space infections and abscesses can vary from mild retropharyngeal pain and malaise to severe respiratory distress and systemic toxicity. Patients often experience an abrupt onset of high fever that is associated with drooling, dysphagia, neck pain on hyperextension, and dyspnea. Respiratory distress can develop because of the anterior displacement of the pharyngeal wall and the supraglottic structures. On transoral examination, bulging of the posterior oropharynx may be seen or palpated, although palpation of the lesion may lead to abscess rupture with aspiration or asphyxiation. The oropharynx can be examined carefully, only in a cooperative patient, who should be placed in the Trendelenburg position. Suction equipment must be ready in the event of abscess rupture. [90,161] The CT scan is the gold standard imaging technique, and plays a critical role in surgical decision-making. Described abnormalities are the presence of fluid-like opacities with rim enhancement, scalloping, gas collections, soft-tissue swelling, and obliterated fat planes.
Management includes intravenous administration of antibiotics and drainage of the abscess. Intraoral approach is currently the preferred route. A needle aspiration might be a sufficient treatment when it retrieves pus. If not, the surgical procedure should be completed by incision and drainage. Most abscesses can be drained by peroral incision and suction. When the risk of airway obstruction is great, tracheostomy may be needed. External incision is required rarely, when the abscess is extending laterally to the great vessels or inferiorly towards the mediastinum.  However, several studies dealing with pediatric population pointed out the poor correlation between CT scan abnormalities and pus finding during the surgery  or reported successful treatment of CT diagnosed abscesses by antibiotics without surgery. 
11.4. Danger space and prevertebral space infections
The danger space is located posterior to the alar fascia and is bounded by the prevertebral fascia posteriorly. It is delineated superiorly by the base of the skull; inferiorly it extends through the posterior mediastinum to the diaphragm. Infections of the danger space usually develop by direct spread from adjacent spaces. Infections of danger and prevertebral space can extend throughout the posterior mediastinum and may involve the retroperitoneum. Occasionally, the purulent material from the posterior mediastinum ruptures into the pleural cavity, causing a pyothorax and secondary pleural effusions. Another feared consequence of mediastinal invasion is pericarditis with pericardial effusion and potentially tamponade.  The prevertebral space is the crevice between the prevertebral fascia and spinal column. It extends from the base of the skull down to the coccyx and is contiguous with the sheath of psoas muscle. Infections of the prevertebral space usually develop from hematogenic osteomyelitis/discitis of the cervical spine. They can also result from iatrogenic penetrating injuries of the trachea or esophagus. Infections of the prevertebral space behave in a different manner from infections of the retropharyngeal and danger spaces. Complications commonly arise from spinal epidural cord compression resulting in paralysis. They can also lead to psoas muscle abscess, because of the open communication of the prevertebral space down to the psoas muscle. Infections of the vertebrae or disc may cause local destruction with mechanical instability of the spine. 
11.5. Bezold’s abscess
Bezold’s abscess occurs when a purulent mastoiditis erodes the bone of the mastoid tip. The infection process is prevented from reaching the skin surface by the intervening neck musculature. When left untreated, the pus can track along the fascial planes of the digastric or sternocleidomastoid muscles and spread downward to the carotid sheath. The classic Bezold’s abscess was first reported in 1881 following a cadaver study in which pus was found to track from the medial side of the mastoid process through the incisura digastrica. Treatment consists of incision and drainage of neck abscess and elimination of mastoid infection in addition to wide spectrum antibiotics.  (Figure 22)
11.6. Cervical Necrotizing Fasciitis
Cervical necrotizing fasciitis (CNF) is a rapidly progressing destructive, polymicrobial infection that spreads alongside deep fascial planes of the neck. It most frequently develops from odontogenic sources, but can be also caused by progression of tonsillar and pharyngeal abscesses, injury to the tissues by a foreign body or catheterization, and postoperative wound infections. If not treated promptly and radically, it can reach the thorax and develop into descending necrotizing mediastinitis. CNF complicated by mediastinitis has 41% mortality according to the recent literature review.  (Figure 23)
General principles of diagnosis and management of NF are described in the previous section dealing with facial skin infections. Early aggressive incision and drainage and debridement, along with close surveillance with repeat CT scans and retreatment, when indicated, are compulsory if any chances for favorable outcome are to be retained. No definitive treatment for descending necrotizing mediastinitis has been established. The primary treatment currently involves drainage through a combined cervical and thoracic approach, although some authors believe that cervical drainage alone is sufficient.