Jerzy Kulski

University of Western Australia Australia

Dr. Jerzy K. Kulski, biochemist and geneticist, received his PhD from the University of Western Australia (UWA) in 1979 for his research on human lactation. He completed two post-doctoral fellowships in the USA (1980-1984), before returning to UWA to investigate the association between human papillomaviruses and uterine cervical cancer. After 1996 he held a number of teaching and research positions including Visiting Professor at Tokai University School of Medicine (TUSOM) in Japan to study the genomics and diseases of the Major Histocompatibility Complex (MHC). Since 2007, he has continued his studies on MHC genotyping by next generation sequencing as an adjunct at UWA in collaboration with researchers at TUSOM. He has more than 180 scientific publications and has authored a crime novel entitled 'China Heist.”

1books edited

2chapters authored

Latest work with IntechOpen by Jerzy Kulski

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

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Next Generation Sequencing

Edited by Jerzy Kulski

Chapters authored

In Phase HLA Genotyping by Next Generation Sequencing — A Comparison Between Two Massively Parallel Sequencing Bench-Top Systems, the Roche GS Junior and Ion Torrent PGM

By Jerzy K. Kulski, Shingo Suzuki, Yuki Ozaki, Shigeki Mitsunaga, Hidetoshi Inoko and Takashi Shiina

Part of the book: HLA and Associated Important Diseases

Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications

By Jerzy K. Kulski

Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation sequencing have impacted enormously on the life sciences. NGS is the choice for large-scale genomic and transcriptomic sequencing because of the high-throughput production and outputs of sequencing data in the gigabase range per instrument run and the lower cost compared to the traditional Sanger first-generation sequencing method. The vast amounts of data generated by NGS have broadened our understanding of structural and functional genomics through the concepts of “omics” ranging from basic genomics to integrated systeomics, providing new insight into the workings and meaning of genetic conservation and diversity of living things. NGS today is more than ever about how different organisms use genetic information and molecular biology to survive and reproduce with and without mutations, disease, and diversity within their population networks and changing environments. In this chapter, the advances, applications, and challenges of NGS are reviewed starting with a history of first-generation sequencing followed by the major NGS platforms, the bioinformatics issues confronting NGS data storage and analysis, and the impacts made in the fields of genetics, biology, agriculture, and medicine in the brave, new world of ”omics.”

Part of the book: Next Generation Sequencing

Jerzy Kulski

Latest work with IntechOpen by Jerzy Kulski

Next Generation Sequencing

Chapters authored

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