Mei-Chen Tseng

By Mei-Chen Tseng, Chuen-Tan Jean, Peter J. Smith and Yin-Huei Hung

Part of the book: Analysis of Genetic Variation in Animals

By Mei-Chen Tseng and Dai-Shion Hsiung

Part of the book: Molecular Approaches to Genetic Diversity

By Mei-Chen Tseng and Kuan-Wei Shih

In this study, karyotypes and Cyt b gene sequences of seven different species of grouper including Plectropomus leopardus, Epinephelus coioides, E. flavocaeruleus, E. fuscoguttatus, E. lanceolatus, E. polyphekadion, and E. tukula were examined. All chromosome numbers from seven groupers were 2n = 48 with a high number of telocentric chromosomes (38–48) and fundamental arm numbers (FNs) (48–54). The mitochondrial Cyt b gene was used to establish the barcodes of seven groupers and analyze phylogenetic relationships among these species. We discovered that Epinephelus groupers should be classified as monophyly. The minimum genetic distance expressed between E. coioides and E. tukula was 0.1276. From results of the cytogenetic and molecular analyses, it was demonstrated that Plectropomus is a relatively primitive genus of grouper, while Epinephelus is a more-modern derived genus. Results also showed that E. coioides and E. tukula have similar genetic characters and karyotypes, and should be foremost considered for artificial hybridization strategies. Furthermore, information on karyotypes of species within the Epinephelus is still insufficient, and further elucidation of karyotypes of Epinephelus will be a great help to future genetic breeding research.

Part of the book: Cytogenetics