Abstract
The efficiency in the treatment of leaf rust of wheat was examined for the plant leaf extracts of neem and Moringa at varied concentrations of 50, 100, and 150 ml correspondingly. All treatments decreased fungal growth in vitro by greater than 90%. The germination of spores was decreased by 91.99% in the presence of neem leaf extract at 150 ml concentration. The percentage of pustules/leaf was reduced by foliar spray of the same treatments on seedlings of the wheat plant. The wheat plants show the greatest response against the pathogen of leaf rust by plant extract second foliar application on the fourth day of infection. Spray application of 150 ml, 100 mL of neem leaf extracts, and 150 ml of Moringa leaf extracts at wheat seedlings and rust development completely prevented four days after leaf rust inoculation. The application of treatments of all extracts on wheat plants at the mature stage significantly reduced the disease (ACI, average infection coefficient) and increased the efficacy of plant extract application as compared with control but neem 150 ml treatment was most effective in all. There was a higher increase of the chlorophyll and phenol content in wheat plants.
Keywords
- Trichoderma herzianum
- leaf rust
- commercial wheat varieties
- plant pathogen
- biocontrol agent
1. Introduction
Wheat (
The rust fungus gives great loss to the production of wheat all over the world where wheat is grown. Only in Asia does it affect about 43–63% of the growing region if susceptible varieties were grown [4].
2. Background study
In this chapter, a brief review of research work is given in a manner to highlight the contemporary status of findings in leaf rust.
Wheat rust was divided into three types: Of the most common wheat rust is leaf rust caused by
2.1 Leaf rust epidemic
In an epidemic situation of leaf rust
The study purpose of the wheat rust disease damage both qualitatively and quantitatively if the susceptible varieties of wheat line/genotype can be managed by the resistant line development. They evaluate the 30 lines against yellow and leaf rust where they do artificial inoculation and some lines were observed under natural conditions to assess the disease severity [7]. By using cobb’s scale method, they observe different rating scales of virulence on the 16 genotypes under a natural condition in comparison with the artificially inoculate rust in wheat lines. The genotypes also show different virulency against leaf rust and yellow rust. The data show that among 30 wheat lines that were inoculated artificially, the resistant and moderately resistant varieties/lines were six in number, while the line/varieties showing MRMS response were 13 and few of the lines showed susceptibility and moderately susceptible. But in natural conditions except from two lines/varieties, others were resistant against leaf and stripe rust. The resistant varieties can be a managed way to manage the leaf and stripe rust so breeders can have a stance on developing resistant varieties.
The surveillance in Pakistan from 2016 to 2018 of leaf rust affects the yield of wheat. A 3-year study design contains 95 districts from which 1202 fields were observed to check the spatial and temporal vigilance of disease severity of leaf rust distributed in the Sindh and Punjab provision of Pakistan. The results of 3-year disease incidence showed the most prevalence of disease in 2017 than in 2016 and 2018. The most affected province is where 60% disease severity occurred in 20% region, while Punjab has only 5% region where south Punjab was most affected and in Khyber-Pakhtunkhwa and Azad Kashmir only 1% disease occurred [8]. Some varieties that show susceptibility were Sehar, Inqlab-91, Shafaqand Morocco.
For the surveillance of rust virulence and disease incidence, their assessment characters are evaluated through the survey of trap plot. The survey is helpful in the seed system, plant breeding, and disease-protecting strategies. Many activities were done at the national level for rust pathogen control but at the global level, the strategies of rust surveillance work very slowly. To make rust surveillance effective at the global level, the Global Cereal Rust Monitoring System GCRMS was recognized to cope with the reinforced problem [9]. The system was a web-based monitoring protocol that will be helpful in testing, disease management, rust virulency, and all the factors that were interrelated for the cause of rust pathogen posing threat to wheat. It also includes surveillance data that will be compared to check the rust pathogen virulency at the global level.
2.2 Susceptibility of commercial wheat varieties
Two-season research was done by Muhammad et al. [10]. During the wheat-growing seasons of 2010–2011 and 2011–2012, 325 genotypes of bread wheat (
How much the severity of the leaf rust disease impacts photosynthetic and grain output in wheat. This was accomplished by calculating the photosynthetic rate, disease severity, chlorophyll content, and wheat reduction in six wheat cultivars grown in uncontrolled and fungicide-treated environments [11]. The mean disease severity level of leaf rust was the greatest on Faisalabad-08 and Galaxy-13 among six wheat varieties/lines such as Faisalabad-08, Galaxy-13, Lasani-08, Millat-11, and two wheat lines NW-3-3341-7 and NW-1-8183-8, although grain yields of wheat were also greater in Millat-11, Galaxy-13, and FSD-08. Fungicide dramatically decreased rust infections and increased chlorophyll concentration and photosynthetic rate, leading to considerably greater production in treated plots. Wheat cultivars FSD-08 and Galaxy-13 were determined to be highly resistant to rust illnesses based on leaf rust severity and yield component assessments. When treated with a rust fungicide, NW-3-3341-7 showed the cheapest and best ratio result. Rust disease drastically reduced grain output, according to the study. When host immunity is combined with little fungicide treatment, the negative effects of leaf rust were reduced and net yields were maximized.
The plants were infected with
The natural conditions were conducive to the establishment of the wheat leaf rust disease. Out of 150 lines/cultivars tested for brown/leaf rust, 29 lines/cultivars were immune, 57 types exhibited resistance, and the rest were vulnerable [13]. The area under the disease progress curves (AUDPCs) estimates of all types were determined. According to the virulency formula investigated, 57 types of leaf rust were resistant and 49 variations were susceptible to leaf rust fungus. Environmental variables have a significant impact on the progression of wheat leaf rust infections. There was also a relationship between disease severity and environmental factors. Many varieties/lines logically responded to environmental variables. Temperature, humidity levels, wind velocity, and rainfall were found to have a substantial impact on illness severity. Even though pathogenicity incidence did not affect the leaf rust virulence. The occurrence of virulence for them is concerning in situations where the genetic foundation of resistance in currently farmed varieties was stumpy.
The most cultivated varieties were Morocco, Pak-81, Fsd-85, Lylpur-73, Inqlab-91, Fsd-83, and WL-711. From 1991 to 1992 to 2000–2001, these varieties were consistently cultivated in a rust trial. Infection was achieved using both natural inoculum and artificial inoculum of the leaf rust
2.3 Some historic bioagents used for controlling leaf rust
Eight plant extracts were used for the biocontrol of leaf rust and compared the efficacy of these plant extracts, that is, neem, white cedar, clove, garlic, garden quinine, Brazilian pepper, black cumin, and anthi mandhaari. By using foliar spray and
Some biological agents against
There are three different domains to control leaf rust because the importance of wheat in the world cannot be ignored. The rust species
The extracts of the different plants are affected to control the leaf rust of wheat. The plants used for biocontrol are
In the agricultural field of research, a study was undertaken to assess the allelopathic effects of leaf extracts of daylight neem leaves on wheat production and its constituents. With the treatment of 0, 50, and 100% liquid leaf extract of neem, grain yield, and several yield attributes of wheat, such as the number of viable tillers, grain/spike, 1000-grain weight, and spike length, no meaningful either promotive or negative impact was seen [19]. The leaf extracts of neem, on the other hand, did not affect wheat yield and yield contributing. The use of a natural weedicide (leaf extracts extract of neem) showed no negative impact on wheat quality.
For evaluating the genetic resistance of wheat lines of 45 genotypes/lines against rust, the lines were inoculated at the booting stage with an aqueous inoculum of wheat. It was a field study in Nepal where the results showed different pathogenicity ratios with high variation of wheat varieties [20]. The surveys of 66 production fields where the old varieties are observed have more disease severity rather than the new and resistant varieties. The prevalence of disease on wheat genotype revealed that leaf rust and yellow rust have a high level of prevalence. Leaf rust has more prevalence than yellow rust but low severity, while yellow rust has high a concern with disease severity. The management of disease and farmer literacy about rust makes the varieties more virulent against disease.
Seed priming using leaf extract and compounds has long been utilized to boost agricultural plant development, but the pathways are still unknown. The goal of this study was to figure out how different seed priming methods in greenhouse wheat work. Hydropriming, moringa aqueous extracts priming, and CaCl2 priming were the seed priming methods utilized. The results revealed that all the seed priming treatments were more efficient than the control at enhancing wheat germinating and seed germination. However,
2.4 Potential of Trichoderma harzianum
Wheat (T. aestivum L.) is one of the most important crops for humans worldwide. Stripe rust disease, caused by
The biocontrol of wheat stem rust
The plant extract of A. indica, bioagent
When compared with the control, all 13 treatments enhanced yield and 1000-grain weight to a larger extent. Fungicide used to has a good effect. Moreover, the leaf extract of neem,
The adoption of resistant varieties is the most cost-effective and efficient means of controlling
The most dangerous disease affecting wheat plants is leaf rust produced by
Under field circumstances, field trials were undertaken on two sowing dates to examine the feasibility of using bioagents to reduce the severity of foliar diseases such as Septoria leaf blotch, powdery mildew, and stem rust.
Many environmental factors were the primary hurdles to attaining the maximum productivity possible in the grain yield. Economic losses due to biotic stressors were predicted to be 26–29% in the region. However, physiological processes have a greater negative impact on crop production that accounts for around 70% of the reduction in yield globally. Pesticides and fertilizers are commonly proven as an efficient control mechanism for wheat crop pests and diseases; however, the build-up of synthetic chemicals inside the soil, plant materials, and fungicide-treated kernels harms human and environmental life [27]. Trichoderma is commercially significant as a biocontrol agent, potentially replacing agrochemicals in the fight against biological and chemical stress.
Various methods such as (
The research was carried out during two consecutive fall seasons in 2012/2014 at the El-Kassasein Experimental Farm, Hort. Res. Station, Ismailia Governorate, Egypt. The effect of three different nitrogen fertilizer sources was as follows: namely ammonium sulfate (20.5% N) at 390.2 kg/fed. (fed. = 0.42 ha.), botanical compost at 6.667 tons/fed., and chicken manure at 2.787 tons/fed. (each equaling 80 kg N/fed.), and five biological control (Trichoderma harzianum, Trichoderma viride, the mixture of Trichoderma harzianum + Trichoderma). When compared with Xera cultivar, Paulista cv. provided the maximum dry weight of shoots per plant and final yield. The use of chicken manure enhanced the dry mass of shoots and the overall yield of snap beans. Foliar treatment of a
Plant diseases are one of the most significant restrictions to crop production and productivity, both in terms of quality and quantity. The use of pesticides remains the primary strategy for mitigating agricultural disease threats. However, because of environmental issues, human health problems, and other risks connected with the use of chemicals, the use of bioagents to reduce the disease-causing activities of plant pathogens is gaining popularity. Biocontrol is the intentional use of living organisms, either transferred or indigenous, other than disease-resistant host plants, to decrease the activities or populations of one or more plant diseases. Beneficial organisms, their genes, and/or products, such as metabolites, are used in biological control to lessen the detrimental impacts of plant diseases and stimulate positive plant responses [30]. A variety of commercial products based on diverse fungal and bacterial antagonists have been recognized at both national and international levels in this direction. These commercial products include Biocon, Biogaurd, Ecofit, FStop, Soilgaurd, and others that include
3. Conclusion
Acknowledgments
We also acknowledge (Lal Hussain Akhtar Director, Regional Agriculture Research Institute, Bahawalpur) for providing their pearls of knowledge with us throughout this research, as well as the reviewers for their contributions.
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