Agricultural and Biological Sciences » "Soybean and Nutrition", book edited by Hany El-Shemy, ISBN 978-953-307-536-5, Published: September 12, 2011 under CC BY-NC-SA 3.0 license

Chapter 23

Assessing Compositional Differences in Soy Products and Impacts on Health Claims

By Joyce Boye and Sabine Ribéreau
DOI: 10.5772/17688

Article top

Assessing Compositional Differences in Soy Products and Impacts on Health Claims

Joyce Boye1 and Sabine Ribéreau1

1. Introduction

In October 1999, the Food and Drug Administration (USA) approved a health claim for soy indicating that consumption of 25 grams of soy protein a day as part of a diet low in saturated fats and cholesterol may help lower the risk of heart disease. Additionally, soy has officially been approved in the UK, Brazil, Malaysia, Japan, Korea, Philippines, Indonesia and South Africa.

Some countries have, however, been slower to approve a health claim and are requiring more substantive evidence. Questions of concern include the impact of processing on the efficacity of soy protein, information on the normal and acceptable levels of isoflavones in soy products, efficacy of soy proteins in which isoflavones have been removed versus soy proteins containing isoflavones in respect to relationship with cardiovascular disease risk reduction, feasibility of consumers to take in 25 g of soy protein a day, effect of antinutritional factors (e.g., trypsin inhibitors, phytates, lectins, lysinoalanine), heat resistance of trypsin inhibitors found in soybeans and the specific effects of soy protein alone compared to soy protein within a matrix (e.g., whole soybean foods and foods made from soy protein ingredients).

These questions are pertinent as there is a wide variety of soyfoods available on the market with different macro and micro nutrient compositions. Answers to these questions will therefore be useful in identifying the specific conditions required for soyfoods to carry a health claim.

Foods prepared from whole soybeans include soymilk and tofu. Soybean derived ingredients include defatted soy flour, soy protein concentrate, soy protein isolate and soy fibre. Soy protein isolates in particular have been very successful commercially and are used extensively today in the production of a large number of foods. Different techniques can be used for making soy protein isolate and the type of processing treatment used can affect the nutrient and physicochemical composition of the isolate (e.g., composition of residual isoflavones, saponins, trypsin inhibitors, phytic acid and minerals). Isoflavones, saponins, trypsin inhibitors and phytic acid are all biologically active molecules and as such variations in their composition can hinder the ability to determine if a reported health benefit is due to the soy protein alone or the presence of these other compounds.

The chapter will provide a brief update on some of the reported health benefits of soy components. An overview of some of the major soyfoods and soy ingredients currently available on the market will be provided along with a detailed list comparing the effects of processing on the composition of different soy products. Requirements for obtaining health claims using the Canadian example, as well as impacts of compositional differences on potential health benefits are further presented.

2. Soybeans and health

Cardiovascular disease (CVD) is one of the leading causes of death in the world today. According to the World Health Organization by 2030, almost 23.6 million people will die from CVDs, mainly from heart disease and stroke (www.who.int/mediacentre). Elevated low density lipoprotein (LDL) is a major cardiovascular disease risk factor. LDL transports cholesterol and triglycerides from the liver to peripheral tissues and arteries and regulates cholesterol synthesis. Retention of cholesterol in arteries can result in the formation of arterial plaques which increases the risk of atherosclerosis, peripheral vascular disease, strokes and heart attacks. Foods that decrease LDL levels will, therefore, increasingly be of interest as health foods. Several reports have shown a decrease in LDL levels and an increase in HDL after consumption of soy (Nilavsen & Meinertz, 1998; Potter et al., 1998; Merritt, 2004; Zhuo et al., 2004; Sacks et al., 2006; Harlanda & Haffnerb, 2008; Taku et al., 2008). Earlier studies showing similar results formed the basis of the current soy health claim in many jurisdictions.

Due to apparent inconsistencies in findings, in 2008 the Weston Price Foundation submitted a petition to the FDA in response to the FDA's request for public comment on the issue claiming that soy protein products are not safe and have no long history of use in the food supply. The organization also claimed the evidence on soy protein and heart disease was contradictory and inconsistent, and that no standard of scientific agreement had been met (http://www.physorg.com/news122663958.html).

A systematic study conducted by the US department of Health & Human Services indicated that while the evidence was weak for other disease outcomes, there is a suggestion of a possible dose-response effect for soy protein for LDL reduction (http://www.ahrq.gov/Clinic/epcsums/soysum.htm).

Using predictive equations along with a meta-analysis to determine whether the heart health claim for soy continues to be justified, Jenkins et al. (2010) concluded that low density lipoprotein cholesterol (LDL-C) reduction attributable to the combined intrinsic and extrinsic effects of soy protein foods ranged from 7.9 to 10.3%. They further concluded from their study that soy remains one of a few food components that reduces serum cholesterol (>4%) when added to the diet.

In a more recent study, Onuegbu et al. (2011) fed 500 mL of soymilk daily to 42 apparently healthy young to middle-aged subjects for a period of 21 days and reported that soymilk consumption significantly reduced mean plasma TC by 11% and LDL-C by 25% and increased mean plasma HDL-C by 20%. The authors also concluded that soy drink could be an important non-pharmacological cholesterol-reducing agent.

Furthermore, Bruckert and Rosenbaum (2011) have also recently reported LDL-cholesterol reduction ranging from -3 to -10% for soy protein and have indicated that dietary recommendations may have important impacts on cardiovascular events as they can be implemented early in life and because the sum of the effect on LDL-cholesterol is far from being negligible.

Another major risk factor for developing CVD is elevated blood pressure (EBP). A major contributor to EBP is Angiotensin II which is a potent vasoconstrictor. Vasoconstriction occurs when renin, an enzyme produced in the kidneys, proteolytically acts on circulating angiotensinogen and converts it to angiotensin I (a decapeptide). In the presence of angiotensin converting enzyme (ACE), angiotensin I is cleaved to the octapeptide, angiotensin II resulting in arterial constriction and EBP. ACE also breaks down bradykinin, a vasodilator, further contributing to the elevation in blood pressure. Inhibition of ACE is, therefore, important for the lowering of blood pressure as this results in a decrease in the concentration of angiotensin II and an increase in the levels of bradykinin (Yang et al., 1970; Erdos, 1975). Various reports have suggested that peptides from soy posses ACE-inhibitory properties (Wu & Ding, 2002; Kuba et al., 2003; Chiang et al., 2006; Hartmann & Meisel, 2007; Yang et al., 2008). These peptides are usually not active when present within the sequence of parent proteins, but are released by enzymatic proteolysis in vivo or in vitro.

In addition to soy proteins, some reports have attributed the beneficial health effect of soy to phytochemicals found in soybeans such as isoflavones which are naturally occurring non-steroid compounds with weak estrogenic effects and chemical structure similar to estradiol-17β (Fig. 1). Isoflavones are able to bind to estrogen receptors and are capable of triggering estrogen dependent responses physiologically. The main types of isoflavones found in soy are the aglycones (daidzein, genistein, and glycitein), the β-glucosides (daidzin, genistin, and glycitin) and their 6″-O-malonyl-β-glucosides (6OMalGlc) and 6″-O-acetyl-β-glucosides (6OAcGlc) conjugates.

Taku et al. (2010) conducted a meta-analysis to clarify the effects of soy isoflavone extracts on systolic and diastolic blood pressure (SBP and DBP) in adult humans. They reported that soy isoflavone extracts significantly decreased SBP but not DBP in adult humans, however, no dose-response relationship was observed.

media/image2_w.jpg

Figure 1.

Molecular structure of soy isoflavone genistein compared to estradiol-17β.

In other studies, high dietary intakes of soy isoflavones were found to be associated with lower risk of recurrence among postmenopausal patients with breast cancer positive for estrogen and progesterone receptor and those who were receiving anastrozole as endocrine therapy (Kang et al., 2010a, b).

Ogborn et al. (2010) have also recently reported that a soy diet ameliorated renal injury in rats. Soy diets preserved normal renal function and reduced relative renal weight, scores for cystic change, fibrosis, tissue oxidized LDL content, inflammation and epithelial cell proliferation. In this study though, alcohol-extracted slow isoflavone soy protein was found to retain its major protective effects and only subtle differences were attributed to isoflavones.

Consumption of tofu containing high levels of isoflavones reportedly exerted positive effects on verbal memory, although not in older men and women, where no or negative effects of these compounds on brain cells and cognition was observed (Hogervorst et al., 2011).

In regards to other health outcomes, Messina (2010) reported that although recent clinical data have not supported the skeletal benefits of isoflavones, 2 large prospective epidemiologic studies found soy intake to be associated with marked reductions in fracture risk. Additionally, soybean isoflavones modestly alleviate hot flashes in menopausal women.

In addition to isoflavones, soy saponins may also exert bioactive effects. Orally administered commercial purified soy saponin at 80 mg/kg body weight/day to spontaneously hypertensive rats for 8 weeks significantly decreased blood pressure (Hiwatashi et al., 2010). In another study on the effect of soy saponins on the growth of human colon cancer cells, Tsai et al. (2010) reported that intake of soy saponin decreased the number of viable cells in a dose-dependent manner. They concluded that soy saponin may be effective in preventing colon cancer by affecting cell morphology, cell proliferation enzymes, and cell growth.

Additionally, phytic acid which is considered to be an antinutritional component in soybean may possess antioxidant effects (Sakač et al., 2010). Recent research studies further suggest that lunasin, lectins, and trypsin inhibitors may have beneficial health properties. Trypsin inhibitors in soy have been of particular concern because, if not destroyed by heat prioir to consumption, they can cause pancreatic hypertrophy/hyperplasia, which ultimately results in growth inhibition (Liener, 1994,1996).

The majority of approved health claim for soy covers only soy proteins as evidence surrounding the effects of isoflavones and other bioactive compouds in soy are more controversial. Overall, although many studies suggest that the beneficial properties of soy may be attributed to the protein fraction, questions remain about potential synergistic or complimentary effects of other soy components.

In the sections below, an attempt will be made to provide a review of some of the different soy products available and their compositional differences and how this could impact their health properties.

3. Commercially available soyfoods and ingredients

Soybean has today become one of the world’s most economical and valuable agricultural commodities due to its unique composition. On a wet basis, soybeans contain about 35% protein, 17% oil, 31% carbohydrate and 4.4% ash. The composition varies for different varieties and some cultivars can be found with protein contents of up to 50%. Soy proteins are nutritionally superior among vegetable proteins. Soy protein isolate has a Protein Digestibility Corrected Amino Acid Score (PDCAAS) of 100% which means that it has all the essential amino acids required to support growth and maintenance. It contains good supplies of essential amino acids, such as lysine, which are normally lacking in other cereals. Fatty acids in soybeans consist of unsaturated fats, such as oleic, linoleic and linolenic acids, which are nutritionally beneficial. Additionally, soybeans also contain fibre and other phytochemicals, such as isoflavones and saponins which may have health benefits.

3.1. Major soyfoods

3.1.1. Soybeans and sprouts

Green soybean and soybean sprouts are two whole soyfoods prepared from soybean seeds. Sprouts are obtained by germinating soybeans for 5-10 days. They may be consumed fresh (e.g., in salads) or used as a vegetable in cooking. Green vegetable soybean, on the other hand, is harvested just before maturity (edamame) and can be cooked and eaten in salads and in soups or as a snack. It is available fresh (in pod or shelled), canned or frozen. The composition of these two products although prepared from the whole seed will vary due to the germination process applied to sprouts.

3.1.2. Soymilk

Soymilk is the liquid extract obtained after cooking, grinding and filtering soybean. It is not a “whole soyfood” per se as the majority of the fibre fraction (okara) is removed during processing. The soymilk extract obtained after filtration has a consistency that is very similar to cow’s milk and is frequently used as an alternative to dairy products. There are four major types of soymilk products available (unsweetened, sweetened, flavoured and low fat). Unsweetened soymilk generally contains only water and soybeans. Sweetened soymilk may be sweetened with rice syrup, honey, corn or barley malt extract. Flavoured soymilk may be sweetened or unsweetened, and is often flavoured with cocoa, vanilla, carob or strawberry. Low fat soymilk may also be sweetened or unsweetened, flavoured or unflavoured, but usually contains less fat. Soymilk is frequently fortified with vitamins and minerals to increase its nutrient value. Blends of soymilk made with soybeans and different cereals or fruits are also available as well as “functional soymilk products” (e.g., with added omega 3 or other functional ingredients). Some manufacturers process soymilk using soy protein isolate rather than starting with the bean.

3.1.3. Tofu

Tofu is a curd made from heated soymilk. It is prepared by adding coagulating agents such as glucono-δ-lactone (GDL) or salts (magnesium chloride, calcium chloride, calcium sulphate) to heated soymilk followed by pressing to remove the whey. The final product is a gel with different textures and degrees of hardness depending on the type and amount of coagulant used and processing method (Fig.2). As with soymilk, tofu is not a “whole food” as the fibre is removed in the process of making the soymilk. The composition is also different from the starting soymilk as much of the whey is removed to concentrate the proteins and facilitate gel formation during pressing. Tofu has a soft white texture which is in some respects similar to cheese. On a wet basis, pressed tofu with a moisture content of about 85% contains 7.8% protein, 4.2% lipid, and 2 mg/g calcium (Wang et al., 1983; Liu, 1997). It is important to mention that in addition to compositional differences due to the type of salts used, health outcomes may also vary due to differences in protein digestibility resulting from the type of network structure induced by the specific salt used in tofu-making.

media/image3_w.jpg

Figure 2.

Microstructure of tofu prepared using different salts.

3.1.4. Other fermented products from soybean

Soy sauce and soy paste (miso) are fermented soy products that are frequently used as condiments and seasoning in foods. Soy sauce and miso are made by fermenting soybean with or without other grains (e.g., wheat, rice, barley) with different types of Aspergillus.

Soy sauce is obtained in a liquid form whereas miso is a thick paste. These products are traditionally used in Asian cuisine but have become a mainstay of many modern diets. Tempeh and natto are two other fermented soyfoods but these are less frequently consumed outside of Asia. Tempeh is made by fermenting dehulled and cooked soybeans with Rhizopus whereas natto is fermented with Baccillus subtilis. The composition of these products will again vary depending on the processing conditions used and the amount of soybean present in the finished product. Furthermore, microorganisms used during fermentation can hydrolyse some phytochemicals such as isoflavones making them more bioactive.

3.1.5. Soy dips/dressings

There is a wide variety of dips/salad dressings etc. made from soybeans, soy flour, tofu, or soy protein isolates available on the market. They contain varying amounts of soy and their composition will similarly vary depending on the other ingredients used in the formulation.

3.1.6. Other soyfoods

The liquid extract during the preparation of soymilk can be further processed into a variety of refrigerated and frozen desserts such as ice cream, soy mousse, and fermented products such as soy yoghurt, soy probiotic beverage, and soy cheese, using processes similar to those used in the dairy industry. The composition of these products will vary as a function of the ingredients used in processing and the fermentation process including the type of bacteria used during fermentation.

Soybeans can also be roasted in a manner similar to peanuts. The product obtained has a nutlike flavour and a crunchy texture which can be consumed as a snack. As these are made from intact whole bean, the composition will be similar to that of the starting raw material, however, the roasting process can induce changes which may modify digestibility. Roasted soybeans can also be ground to obtain roasted soy flour or roasted soynut butter.

3.2. Major soy ingredients

3.2.1. Soy oil

Today soybean oil is one of the world’s leading vegetable oil for human consumption. Soybean oil is extracted from the bean after dehulling and flaking using organic solvents. The extracted oil is downstream processed to obtain a refined oil. Soybean oil is widely used in the manufacture of different foods. It is also frequently used as a salad or cooking oil and in the production of shortening, margarines, mayonnaise and salad dressings. By-products from the processing of soybean oil are also used to produce mono- and diglycerides and lecithin which are commonly used as emulsifying agents in foods.

3.2.2. Soy flour, soy protein concentrates, isolates and hydrolysates

Extraction of oil from soybeans leaves behind the soy meal biomass. Significant effort has been made in the last few decades to process this meal into value-added products. Defatted soy flakes (or flour, grits, meal), soy protein concentrates (SPC) and soy protein isolates (SPI) are the three major products available from soy meal. Additionally a full fat or partially defatted meal or flour can be obtained from the whole soybean.

Defatted soy flakes contain approximately 50% protein while, SPC and SPI generally contain at least 65% and 90% protein on a dry basis, respectively. Full-fat soy flour may be steamed and toasted to inactivate enzymes and enzyme inhibitors or unheated. Unheated, undefatted (or defatted) soy flour gives an enzyme active full-fat soy flour which is used in the bakery industry to bleach flour. Mechanically defatted soy meals with different fat contents are also available and usually sold in the organic category. Micronized soy flour (heat treated with infrared to eliminate anti-nutritional components in soy) are also available as whole beans or ground.

Soy products are widely used in formulated foods, partly because of their nutritional value but especially for the functional properties of the protein, which include gelling, foaming and emulsification which underlie many food sensory attributes. Other food products likely to contain soy ingredients include beverages, nutritional bars, bakery and cereal products, soups, meat products, beverages, confectionery, salad dressings and desserts. A growing use of soy protein concentrates and isolates is in the preparation of texturized food products that are used as meat alternatives or in cereal products. Soy proteins can also be hydrolysed enzymatically or chemically to produce hydrolysed vegetable protein (HVP) which is used as a flavour enhancer in many foods.

3.2.3. Other soy ingredients

In addition to the products listed above, soy dietary fibre, soy hulls, soy isoflavone extracts, vitamin E and soy phytosterols are examples of other ingredients from soybean processing which are used in nutraceutical products.

4. Compositional differences in soy products

The macro and micro nutrient composition of different soy products will vary markedly depending on soybean variety and the attendant biotic and abiotic influences, processing condition, other ingredients used in processing and whether the whole soybean or specific components are used in product development. Tables 1 to 8 provide detailed lists showing some of the compositional differences of a variety of soy products. As can be seen in the tables, the composition of protein, isoflavones, mineral, trypsin inhibitors, phytic acid and saponins can vary markedly for different soy products.

Additionally, it is important to note that even within a particular food category the type of processing technique and conditions used can impact composition and potential health benefits. We have showed in some of our earlier papers that the conditions used for processing of soy protein concentrates and isolates (such as particle size, method of defatting, process pH) can influence final product composition (Russin et al., 2007; L’hocine et al., 2006). Furthermore, Sobral et al. (2010) also reported that methods and conditions of preparation and storage of protein samples and mixtures of proteins were factors that modified their thermal behavior. In some instances higher denaturation temperatures increased thermal stabilization of soybean storage proteins which was attributed to protein-protein interactions occurring during processing. This increased stabilisation can impact digestibility and potentially, bioactivity.

Conditions used during spray drying can affect product composition. An increase of the inlet air temperature during spray drying of fermented soymilk greatly reduced viability and isoflavone aglycone content (Telang & Thorat, 2010). Moreover, denaturation of proteins during processing further reduced product solubility.

Processing of soybeans under severe alkaline conditions could lead to the formation of lysinoalanine, which can have negative impacts on health and decrease the bioavailability of essential amino acids (i.e., lysine). Processing under milder alkaline conditions avoids the formation of lysinoalanine and reduces potential negative side effects (Liener, 1994).

Additionally, the temperature and time used during the germination of soybeans in the production of germinated soybean flour modified the concentrations of bioactive compounds (i.e., isoflavones, saponins, trypsin inhibitors and lectins) (Paucar-Menacho et al., 2010a). At 25 °C, an increase in germination time decreased the concentration of Bowman-Birk inhibitor, lectin and lipoxygenase. Optimal increases in the concentrations of isoflavone aglycones (daidzein and genistein) and saponin glycosides were observed with a 63 h germination time at 30 °C. In a second study the authors found that germination of soybean for 42 h at 25 °C increased lunasin concentration by 62% and decreased the content of lectins by 59% (Paucar-Menacho et al., 2010b). Germination at 25 °C for 42 h resulted in a 32% increase in the concentration of soy saponins.

Similarly, fermentation of soymilk using a variety of probiotic lactic acid bacteria (LAB) resulted in the production of beta-glucosidase which hydrolyzed isoflavone glucosides to the bioactive isoflavone aglycones, genistein and daidzein in the fermented soymilk (Rekha & Vijayalakshmi, 2010a, 2011). Furthermore, decreases in phytic acid and increases in mineral bioavailability (e.g., calcium) were also observed. Huang et al. (2011) prepared sufu, a fermented soybean curd, by ripening salted tofu cubes in Aspergillus oryzae-fermented rice-soybean koji mash at 25, 35 or 45 °C for a period of 16 days and found that regardless of temperature, ripening caused a major reduction in the content of β-glucoside and malonylglucoside isoflavones along with a significant increase of aglycone isoflavone

Soy productMoisture %Protein %Fat
%
Carb.4 %Fiber
%
Ash %Reference
Fresh soybean681361122Snyder & Know, 1987
Soybean (dry)7.50-10.131.1-36.616.3-21.36.2922.04.69Souci et al., 2000
Soybean (dry)db39.5-40.120.4-21.014.7-17.221.1-21.75.3-5.4Van der Riet et al., 1987
Soybean sprouts 8282811Snyder & Know, 1987
Soy curd8863200.6Snyder & Know, 1987
Firm tofu79.3 - 75.510.6-14.3Shurtleff & Aoyagi, 2000
Edam (cheese)41.028.925.01.43.7Ono, 2003
Ganmodoki (tofu derivative, td)63.515.317.81.61.8Ono, 2003
Fried bean curd (td)75.910.711.30.91.2Ono, 2003
Dried frozen tofu (td)8.149.433.25.73.6Ono, 2003
Tempeh unfermented soybeans1 db48.325.7-28.62.0-2.817.7-19.33.0Van der Riet et al., 1987
Tempeh fermented (24h)db48.7-49.725.2-27.91.9-2.418.3-20.22.7-2.8Van der Riet et al., 1987
Tempeh fermented (48h)db48.6-49-725.2-26.31.9-2.216.1-19.32.7Van der Riet et al., 1987
Tempeh fermented (72h)db49.322.9-23.81.7-2.315.3-15.73.0Van der Riet et al., 1987
Tempeh (FWB)264184131Snyder & Know, 1987
Natto (FWB) 25917101223Snyder & Know, 1987
Hamanatto (FWB) 236261214312Snyder & Know, 1987
Tempeh Gembus (FSP) 38152111Snyder & Know, 1987
Oncom ampas tahu (FSP) 3844282Snyder & Know, 1987
Soy sauce7270.52018Snyder & Know, 1987
Fermented soy curd6017140.19Snyder & Know, 1987
Soy paste5014516215Snyder & Know, 1987
Ko Chu jang (soy paste)489419420Snyder & Know, 1987

Table 1.

Composition of different soy foods

1 Before fermentation; 2 FWB-Fermented whole soybean; 3 FSP-Fermented soy pulp; 4 Carbohydrate.
Soy productMoisture
%
Protein
%
Fat
%
Carb.1
%
Ash
%
Reference
Soymilk 943110.3Snyder and Know, 1987
Soymilk 88.73.201.845.760.48Souci et al., 2000
Soy milk (full fat) dried<103818<7Garcia et al.,1997
Soy milk (low fat) dried<5489<5Garcia et al.,1997
Soymilk film95224123Snyder and Know, 1987
Soy ice cream 91.12 - 92.453.12 – 4.081.78 – 1.971.89 – 2.660.46 – 0.85Sutar et al., 2010

Table 2.

Composition of soymilk and soymilk derived products

1 Carbohydrate
Soy productMoisture
%
Protein
%
Fat
%
Carbohydrate
%
Ash
%
Reference
Defatted soy meal53130Achouri et al., 2005
Soya flour (full fat)8.94-9.5435.9-38.819.8-22.13.104.40Souci et al., 2000
Soyflour (whole)50Hoogenkamp, 2001
Soyflour (natural)40Shurtleff & Aoyagi, 1983
Soyflour (full fat) dried<1042214.7Garcia et al.,1997
Soyflour (defatted)6-852-540.5-1.030-325.0-6.0Endres, 2001
Soyflour (defatted)51Shurtleff & Aoyagi, 1983
Soyflour (defatted) dried6-842-520.5-1.05-6Garcia et al.,1997
Toasted soy flour 5381932 5Snyder & Know, 1987
Soya protein concentrate4-662-690.5-1.03.8-6.2Garcia et al.,1997
Soy protein isolate4-686-870.5-1.03-43.8-4.8Endres, 2001
Soy protein isolate4-686-870.5-1.03.8-4.8Garcia et al.,1997

Table 3.

Composition of selected soy ingredients

1 From defatted soybean
media/image4_w.jpg

Table 4.

Isoflavone content of various soy foods

Source: Reprinted with permission from Shimoni, 2004. (DEN – daidzein; DIN – daidzin; MDN – malonyl daidzin; ADN – acetyl daidzin; GEN – genistein; GIN – genistin; MGN – malonyl genestin; AGN – acetyl genistin; GEIN – glycitein; GIIN – glycitin; MGIN – malonyl glycitin; AGIN – acetyl genistin)
Soy productPotassiumPhosp.1CalciumMagn.1IronZincMagn.1SodiumCopperRef.
mg/100g
Soybean1693-1739635-830187-275247-28211.6-11.74.5-6.02.8-4.13.5-9.31.7-1.82
Tempeh unfermented393-525609-627192-296168-1727.2-8.04.5-6.23.3-3.713.8-16.11.6-1.92
Tempeh fermented soybeans (24h)222-224688-731225-310193-2218.7-8.95.4-7.13.5-4.114.2-14.61.8-1.92
Tempeh fermented soybeans (48h)220-224731-742232-333193-1959.05.5-7.23.5-4.114.11.8-1.92
Tempeh fermented soybeans (72h)218-219734-795248-318193-2018.7-8.85.4-7.43.6-4.213.8-15.11.8-1.92
Defatted soy four2400-2700700-900200-300200-3001053-43-151-23
Soy protein concentrate100-2400600-900200-40030010-20552-12001-23
Soy protein isolate100-1400500-800100-20030-9010-204-9240-12001-23

Table 5.

Mineral content of some soy products

1 Phosp. – phosphorous, Magn. – magnesium, Mang. - manganese;

2 Van der Riet et al., 1987;

3 Endres,2001

content. The highest increase in aglycone content and greatest decrease in malonylglucosides was observed at 45 °C and increasing ripening time further enhanced the changes observed.

The composition and antioxidant property of tofu is also affected by processing and the type of coagulant used (Rekha & Vijayalakshmi, 2010b). Tofu prepared with natural coagulants (Citrus limonum, Garcinia indica, Tamarindus indica, Phyllanthus acidus and Passiflora edulis) had significantly higher antioxidant activity compared to those prepared with salts. Furthermore, higher total crude protein and fat contents were found in some of the tofu prepared using the plant based coagulants (G. indica and T. indica).

Differences were reported in the composition and properties of soy whey (aqueous extract of defatted soybean flour) and tofu whey (liquid industrial residue from tofu production) (Sobral & Wagner, 2009), two products that may appear to be similar in properties. Although both byproducts contain primarily carbohydrates, proteins, non-protein nitrogen and salts, tofu whey reportedly had lower amounts of dry matter and proteins, and the antitryptic activity was three times lower than in soy whey. Thermal studies conducted using differential scanning calorimetry (DSC) of soy whey proteins showed endotherms corresponding to lectin and antitryptic factors of Kunitz and Bowman-Birk, whereas the thermogram for tofu whey only showed the presence of antitryptic factors. The authors attributed these differences to the manufacturing processes used. The extent to which these differences can affect health outcomes is unclear.

Soy productTrypsin inhibitor activity
mg/g sample
Reference
Whole soybean16.7-27.2Hafez, 1983
Whole soybean48.2Miyagi et al., 1997
Raw soy flour28-32Rackis et al., 1985
Raw soy flour52.1Anderson et al., 1979
Toasted soy flour7.9-9.4Rackis et al., 1985
Toasted soy flour3.2-7.9Anderson et al., 1979
Soy protein concentrate5.4-7.3Peace et al., 1992
Soy protein concentrate6.3-13.9Anderson et al., 1979
Soy protein concentrate4.4-7.3Peace et al., 1994
Soy protein isolate1.2-30.0Peace et al., 1992 Rackis et al., 1985
Soy protein isolate4.4-11.0Anderson et al., 1979
Soy based infant formulas0.3-2.7Peace et al., 1992
Soy tofu0.6Doell et al., 1981
Soy tofu1.2-3.8Miyagi et al., 1997
Soy milk6.3Miyagi et al., 1997
Soy sauce0.3Doell et al., 1981
Soy miso4.1Doell et al., 1981
Soy food fiber6.47Anderson et al., 1979
Chicken analog*3.63Anderson et al., 1979
Ham analog*5.36Anderson et al., 1979
Beef analog*3.42Anderson et al., 1979
Textured soy flour5.15Anderson et al., 1979

Table 6.

Trypsin inhibitor activity of different soy products

* manufactured using soy protein products Cited from the following sources: Anderson & Wolf, 1995; Snyder and Know, 1987 (calculated on the basis of 1.9TUI = 1 g TI); Gilani et al., 2005.
Soy productPhytic acid %
(dry basis)
Reference
Soybean1.12-1.80Toda et al., 2006
Soybean1.00-1.47Lolas et al., 1976
Soybean1.32-2.30Raboy et al., 1984
Whole soybean (dry)1.07Sutardi & Buclke,1985a
Soybean, raw1.41Sudarmadji & Markakis, 1977
Soaked (24h)1.69Sutardi & Buclke,1985a
Soybean, soaked1.43Sudarmadji & Markakis, 1977
Soybean, boiled (5 min)1.68Sutardi & Buclke,1985a
Soybean, boiled1.23Sudarmadji & Markakis, 1977
Soybean soaked (24h)1.67Sutardi & Buclke,1985a
Soybean (dehulled)1.65Sutardi& Buclke,1985a
Soybean (steamed 30 min)1.48Sutardi & Buclke,1985a
Soybean (drained and cooled)1.47Sutardi & Buclke,1985a
Full fat soy flour1.51-1.81Ranhotra et al., 1974
Defatted soy flour1.62-1.85Ranhotra et al, 1974
Defatted soy flour1.30-1.63Schuster & Bodwell, 1980
Textured soy flour1.10-2.02Davies & Reid, 1979
Textured vegetable protein0.95-1.63Harland & Oberleas, 1977
Concentrate1.25-2.17Ranhotra et al, 1974
Textured concentrate1.48-1.50Harland & Oberleas, 1977
Soy protein isolate0.97-1.69Schuster & Bodwell, 1980
Soy protein isolate1.61-2.00Honig et al., 1984
Spun isolate fiber1.48O’Neill et al., 1980
Soymilk1.68Omosaiye & Cheryan, 1979
Soymilk1.83Beleia et al., 1990
Tofu1.5-2.5Van der Riet et al., 1989
Tofu1.96-2.88Schaefer & Love, 1992
Okara (residue from soymilk)0.5-1.2Van der Riet et al., 1989
Tempeh0.69-0.73Sutardi & Buckle, 1985b
Tempeh unfermented soybeans (before fermentation)1.0-1.2Van der Riest et al., 1987
Tempeh fermented soybeans (24h)0.3-0.6Van der Riest et al., 1987
Tempeh fermented soybeans (48h)0.2-0.4Van der Riest et al., 1987
Tempeh fermented soybeans (72h)0.1-0.2Van der Riest et al., 1987
Tempeh (fresh)*0.68-0.75Sutardi & Buclke,1985a
Tempeh0.96Sudarmadji & Markakis, 1977
Fried fresh Tempeh*0.35-0.38Sutardi & Buclke,1985a
Tempeh stored 2wk@5°C*0.18-0.19Sutardi & Buclke,1985a
Fried stored tempeh*0.09-0.10Sutardi & Buclke,1985a

Table 7.

Phytic acid content of different soy products

* Different types of inoculation used in productionData taken from the following source: Anderson & Wolf, 1995
Soy productSaponin content
%
Reference
Soybean0.44-0.49Berhow et al., 2006
Soybean0.47Ireland et al., 1986
Soybean0.225-0.298Kitagawa et al., 1984
Soybean5.1Fenwick & Oakenfull, 1981
Soybean3.9Fenwick & Oakenfull, 1983
Soybean0.46-0.50Gestetner et al.,1966
Soybean5.6Fenwick & Oakenfull, 1981
Toasted, defatted soy flour0.67Ireland et al., 1986
Defatted soy flour2.0Fenwick & Oakenfull, 1981
Defatted soy flour0.35Curl et al.,1985
Defatted soy flour2.2-2.5Fenwick & Oakenfull, 1981
Commercial soy flour0.46Price et al.,1985
Soya hulls2.0Fenwick & Oakenfull, 1981
Full fat, enzyme active soy flour0.43Ireland et al., 1986
Full fat, heat treated soy flour0.49Ireland et al., 1986
Soya protein isolate0.76Ireland et al., 1986
Soy protein isolate : Promine D*0.3Fenwick & Oakenfull, 1981
Soy protein isolate : GL-750*0.8Fenwick & Oakenfull, 1981
Soy protein isolate : Maxten C*1.9Fenwick & Oakenfull, 1981
Soy protein isolate : Maxten E*2.5Fenwick & Oakenfull, 1981
Soymilk 0.026Ireland et al., 1986
Soymilk 0.022Ireland et al., 1986
Soymilk 0.39Kitagawa et al., 1984
Yuba (dried soymilk film)0.41Kitagawa et al., 1984
Okara (residue of soymilk)0.10Kitagawa et al., 1984
Tofu2.1Fenwick & Oakenfull, 1981
Tofu0.30-0.33Kitagawa et al., 1984
Miso0.15Kitagawa et al., 1984
Natto0.25Kitagawa et al., 1984
Tonyu (soya milk)0.047Kitagawa et al., 1984

Table 8.

Saponin content of different soy products

*trademark

In addition to processing, various plant breeding techniques have been used to modify the composition of soybeans for different food applications (Esteves et al., 2010, Brune et al., 2010). Thus depending on the type of variety used, compositional differences can be expected in macro and micro components (i.e., protein, indispensable, dispensable and total amino acid, lunasin, isoflavones, phytic acid, oxalate and trypsin inhibitors as well as mineral content). The biological activity of soybean products will, therefore, not only depend on the processing technique used but the variety of soybean used.

5. Soyfoods and health claims

An important factor that may contribute to inconsistencies in the evidence of the health benefit of soy may be related to the type of material used for clinical studies. Reinwald et al. (2010) argue the possibility that whole soy may have a more unique effect on health than a select soy component.

Differences in health outcomes related to soy and soy component consumption could be due to varietal and compositional differences, the impact of processing, additive effects of various components (i.e., the whole is greater than the sum of the parts) as well as age and health status. Furthermore, some have argued that the presence or absence of specific gut microflora could also contribute to health outcomes (Patisaul & Jefferson, 2010) as microorganisms may play a critical role in converting physiologically inactive phytochemicals to the bioactive form.

As an example, Reinwald and Weaver (2010) have reported that whereas epidemiological studies in Asia evaluating diets containing traditional whole soyfoods showed a positive association with bone mineral density and fracture protection, smaller scale intervention studies in Western nations mainly using isolated soy protein (SP) and purified or concentrated soy isoflavones (SI) rather than whole soyfoods have produced inconsistent results.

Similarly, Lagari and Levis (2010) found that clinical trials are conflictive regarding the effects of phytoestrogens on bone mineral density and bone turnover markers in premenopausal and postmenopausal women and argue that much of the controversy lies in differences in study design, reporting of results, participants' age and menopausal status, and type and dose of phytoestrogen used.

In Canada, where a health claim for soy has not yet been approved, the ministry responsible for health (Health Canada) requires the following evidence to substantiate health claims:

  1. Causality – Evidence of high quality and quantity of original research in humans based on randomized controlled intervention and / or prospective observational studies are mandatory to substantiate the health claim of food or food constituents with high level of certainty (statistical significance achieved at p≤0.05) and the relationship between the amount of food and the health effect.

  2. Generalizability - The claimed effect of the food of food constituent is biologically / physiologically relevant and expected to benefit the health of the target population, appropriate and validated surrogate marker must be used to ensure the biological relevance and it should be part of the causal pathway between the food and the health outcome.

  3. Quality assurance - The food is produced according to quality standards and consistently meets predefined specifications.

  4. Safety - The subject of a health claim application must be for a food approved for safe use; or, if a novel food is the subject of the health claim, a novel food application must be completed and submitted to Health Canada preceding or concurrent with this application. The adverse effects related to food intake observed in human studies should be addressed and must provide risk management strategies to overcome the adverse effects and or restriction on use of food.

As there is a wide variety of soy products available on the market, the specific type used for clinical studies can impact results.

A major challenge for health claim support studies is the lack of standardized materials and controls for clinical studies as well as the use of appropriate clinical outcomes and surrogate endpoints for different disease risks. Furthermore, very few studies have been conducted to understand what happens to soyfoods and soy components in the gastrointestinal tract and the specific events that occur at the mucosal barrier and how these events influence absorption, distribution, metabolism and excretion. The gastrointestinal mucosa is an interesting and complex system that acts as a barrier between the body and the lumenal environment and is selective in that it allows the transfer of selected nutrients across the epithelium while excluding perceived harmful components in the bolus. This complex interplay between bioactives and the intestinal mucosa also needs to be carefully documented to support any eventual health claim. Matrix interactions can influence the bioactivity of the components of interest and the functional properties of the matrix. Furthermore, for double blind placebo controlled studies which are the gold standard in clinical studies, the effect of the matrices used in the clinical trials need to be carefully evaluated.

As policy makers, food regulators, industry and consumers in other jurisdictions demand more evidence to support current and future health claims, some question of interest that remain include the following:

  • What is the relationship reported between the exposure to soy and specific health effects from both observational and intervention studies that meet selection criteria?

  • Can a clear distinction be made between the effect of soy protein alone and soy protein within a matrix (e.g., whole soybean foods and foods made from soy protein ingredients)?

  • What is the efficacy of soy proteins in which isoflavones have been removed versus soy proteins that contain isoflavones in respect to relationship with cardiovascular disease risk reduction and other health claims?

  • How are breakdown products absorbed in the gastrointestinal tract and how do they exert physiological effects in the body?

  • How do matrix effects influence adsorption, digestion, metabolism and excretion?

  • What is the impact of processing on the efficacy of soy protein and the bioactivity of materials used in clinical trials?

  • What are the ideal samples and controls that should be used in clinical trial studies for specific clinical outcomes and what surrogate endpoints should be used?

  • What is the feasibility for consuming recommended intake levels for health claims especially in populations that do not consume soy frequently (e.g., is the consumption of 20-25 g of soy protein a day feasible?)?

  • What is the documented effect of anti-nutritional factors such as trypsin inhibitors, phytates, lectins and lysinoalanine and the extent of heat resistance of trypsin inhibitors found in soybeans?

6. Conclusion

Soy protein has officially been approved in several countries as a “functional food”, making it one of the most valuable vegetable proteins in the world today. As concerns about health, climate change and the impacts of agricultural practices increase, assurance of a diversified sustainable source of nutrition that provides proven health benefits will become increasingly important. Soybeans can contribute to this nutrient biodiversity in an instrumental way. In crop production and rotation, soybeans play a crucial role in nitrogen fixation making them an important component of agricultural sustainability. Furthermore, soy proteins have excellent functional properties that can be exploited in various food applications. Processing technologies are continually being investigated for soy protein fractionation which will allow modifications in protein profile and which could dramatically improve suitability of soy protein products for targeted food, nutraceutical and industrial applications (e.g., enriched 11S, 7S, or 2S soy protein extracts). Breeding efforts to remove or enhance mico nutrients and other bioactive components are also likely to have an impact on improving health outcomes. To support health claims and enhance the benefits of consumption, constant evaluation of the totality of the body of knowledge in regards to potential health benefits and well designed experimental studies using well characterized materials will, therefore, be needed.

References

1 - A. Achouri, J. I. Boye, D. Belanger, 2005 Soybean isoflavones: Efficacy of extraction conditions and effect of food type on extractability. Food Research International, 38, 1199 1204
2 - R. L. Anderson, J. J. Rachkis, W. H. Tallent, 1979 Biologically active substances in soy products, In Soy Protein and Human Nutrition, Editors: Wilcke H.L., Hopkins D.T. Waggle D.H., eds. Academic Press, New York.
3 - R. L. Anderson, W. J. Wolf, 1995 Overview of soy processing and products: Compositional changes in trypsin inhibitors, phytic acid, saponins and isoflavones related to soybean processing. Journal of Nutrition, 125, 581S 588S
4 - A. Beleia, E. I. Ida, T. T. Lethi, 1990 Phosphorus and pytic acid distribution during soy milk processing. Brazilian Archives of Biology and Tecnology (Arq.Biol. Technol), 33, 623 629
5 - M. A. Berhow, Kong. S. Bin, K. E. Vermillion, M. S. Duval, 2006 Complete quantification of group A and group B soyasaponins in soybeans. Journal of Agricultural and Food Chemistry, 54 (6), 2035 2044
6 - E. Bruckert, D. Rosenbaum, 2011 Lowering LDL-cholesterol through diet: Potential role in the statin era. Current Opinion in Lipidology, 22(1), 43, 48 64
7 - M. F. S. S. Brune, M. S. Pinto, M. C. G. Peluzio, M. A. Moreira, E. G. de Barros, 2010 Biochemical and nutritional evaluation of a soybean line lacking the kunitz trypsin inhibitor and lectins. Ciencia e Tecnologia de Alimentos, 30(3), 657 663
8 - W. D. Chiang, M. J. Tsou, Z. Y. Tsai, T. C. Tsai, 2006 Angiotensin I-converting enzyme inhibitor derived from soy protein hydrolysate and produced by using membrane reactor. Food Chemistry, 98, 725 732
9 - C. L. Curl, K. R. Price, G. R. Fenwick, 1985 The quantitative estimation of saponin in pea (pisum sativum L) and soya (Glycine max). Food Chemistry, 18, 241 250
10 - N. T. Davies, H. Reid, 1979 An evaluation of the phytate, zinc, copper, ion and manganese contents of, and Zn availability from, soya-based textyured-vegetable-protein meat-substitutes or meat-extenders. British Journal of Nutrition, 41, 579 589
11 - J. E. Endres, 2001 Soy protein products- Characteristics, nutritional aspects, and utilization, revised and expanded Edition, a publication of AOCS press, P.O. Box 3489 Champain, IL. 61826 3489USA- 1-89399-727-8
12 - E. G. Erdos, 1975 Angiotensin I-converting enzyme. Circulation Research, 36, 247 255
13 - E. A. Esteves, H. S. D. Martino, F. C. E. Oliveira, J. Bressan, N. M. B. Costa, 2010 Chemical composition of a soybean cultivar lacking lipoxygenases (LOX2 and LOX3). Food Chemistry, 122(1), 238 242
14 - D. E. Fenwick, D. Oakenfull, 1981 Saponin content of soya beans and some commercial soya bean products. Journal of the Science of Food and Agriculture, 32, 273 278
15 - D. E. Fenwick, D. Oakenfull, 1983 Saponin content of food plants and some prepared foods, Journal of the Science of Food and Agriculture, 34, 186 191
16 - M. C. Garcia, M. Torre, M. L. Marina, F. Laorda, 1997Composition and characterization of soyabean and related products, Critical Reviews in Food Science and Nutrition, 37(4), 361391
17 - B. Gestetner, Y. Birk, A. Bondi, Y. Tencer, 1966 Soya bean saponins- VII. A method for the determination of sapogenin and saponin contents in soya beans. Phytochemistry, 5, 803 806
18 - G. S. Gilani, K. A. Cockell, E. Sepehr, 2005 Special guest editor section: Effets of antinutritional factors on protein digestibility and amino acid availability in foods. Journal of the Association of Analytical Chemists International, 88(3), 967-987.
19 - B. F. Harland, D. Oberleas, 1977 A modified method for phytate analysis using an ion-exchange procedure: application to textured vegetable proteins. Cereal Chemistry, 54, 827 832
20 - J. I. Harlanda, T. A. Haffnerb, 2008 Systematic review, meta-analysis and regression of randomised controlled trials reporting an association between an intake of circa 25g soy protein perd ay and blood cholesterol. Atherosclerosis, 200, 13 27
21 - R. Hartmann, H. Meisel, 2007 Food-derived peptides with biological activity: from research to food applications. Current Opinion in Biotechnology, 18
22 - K. Hiwatashi, H. Shirakawa, K. Hori, Y. Yoshiki, N. Suzuki, M. Hokari, M. Komai, S. Takahashi, 2010 Reduction of blood pressure by soybean saponins, renin inhibitors from soybean, in spontaneously hypertensive rats. Bioscience, Biotechnology and Biochemistry, 74(11), 2310 2312
23 - E. Hogervorst, F. Mursjid, D. Priandini, H. Setyawan, R. I. Ismael, S. Bandelow, T. B. Rahardjo, 2011 Soy consumption may be associated with better recall in younger, but not in older, rural indonesian elderly. Brain Research, 1379, 206 212
24 - D. H. Honig, W. J. Wolf, J. J. Rackis, 1984 Phytic acid and phosphorus content of various soybean protein fractions. Cereal Chemistry, 61, 523 526
25 - H. W. Hoogenkamp, 2001 Soy protein & meat formulations, In Lifestyle Marketing Dynamics Coupled With Modern Processing Techniques and Old Time Favorites, published by Protein Technologies International- A DuPont Business P.O. Box 88940, St Louis, MO 63188.
26 - Y. Huang, T. Lu, C. Chou, 2011 Ripening temperature affects the content and distribution of isoflavones in sufu, a fermented soybean curd. International Journal of Food Science and Technology, 46(2), 257 262
27 - P. A. Ireland, S. Z. Dziedzic, M. W. Kearsley, 1986 Saponin content of soya and some commercial soya products by means of Hight-performance liquid chromatography of sapogenins. Journal of the Science of Food and Agriculture, 37, 694 698
28 - D. J. A. Jenkins, A. Mirrahimi, K. Srichaikul, C. E. Berryman, L. Wang, A. Carleton, S. Abdulnour, J. L. Sievenpiper, C. W. C. Kendall, P. M. Kris-Etherton, 2010 Soy protein reduces serum cholesterol by both intrinsic and food displacement mechanisms. Journal of Nutrition, 140(12), 2302S 2311S
29 - J. Kang, T. M. Badger, M. J. J. Ronis, X. Wu, 2010a Non-isoflavone phytochemicals in soy and their health effects. Journal of Agricultural and Food Chemistry, 58(14), 8119 8133
30 - X. Kang, Q. Zhang, S. Wang, X. Huang, S. Jin, 2010b Effect of soy isoflavones on breast cancer recurrence and death for patients receiving adjuvant endocrine therapy. Canadian Medical Association Journal, 182(17), 1857 1862
31 - I. Kitagawa, M. Yoshikawa, T. Hayashi, T. Taniyama, 1984 Quantitative determination of soyasaponins in soybeans of various origins and soybean products by means of high performance liquid chromatography. Yakugaku Zasshi, 104 275 279
32 - M. Kuba, K. Tanaka, S. Tawata, Y. Takeda, M. Yasuda, 2003 Angiotensin I-converting enzyme inhibitory peptides isolated from tufuyo fermented soybean food. Bioscience Biotechnology and Biochemistry, 67, 1278 1283
33 - V. S. Lagari, S. Levis, 2010 Phytoestrogens and bone health. Current Opinion in Endocrinology. Diabetes and Obesity, 17(6), 546 553
34 - L. L’Hocine, J. I. Boye, Y. Arcand, 2006 Composition and functional properties of soy protein isolates prepared using alternative defatting and extraction procedures. Journal of Food Science, 71(3), C137 C145
35 - I. E. Liener, 1994 Implications of antinutritional components in soybean foods. Critical Reviews in Food Science and Nutrition, 34(1), 3167
36 - I. E. Liener, 1996 Effects of processing on antinutritional factors in legumes: The soybean case. Archivos latinoamericanos de nutrición, 44(4 Suppl 1), 48S54S
37 - K. Liu, 1997 Soybeans: Chemistry, Technology and Utilization. Aspen Publishers, USA Originally published by Chapman and Hall, New York, USA.
38 - G. M. Lolas, N. Palamidis, P. Markakis, 1976 The phytic acid total phosphorus relationship in barley, oats, soybeans and wheat. Cereal Chemistry, 53, 867 871
39 - J. C. Merritt, 2004 Metabolic syndrome: soybean foods and serum lipids. Jouranl of the National Medical Association, 96, 1032 1041
40 - M. Messina, 2010 Insights gained from 20 years of soy research. Journal of Nutrition, 140(12), 2289S 2295S
41 - Y. Miyagi, S. Shinjo, R. Nishida, C. Miyagi, K. Takamatsu, T. Yamamoto, S. Yamamoto, 1997 Journal of Nutritional Science and Vitaminology, 43, 575 580
42 - K. Nilavsen, H. Meinertz, 1998 Variable response to dietary soy protein in healthy normolipidemic men. American Journal of Clinical Nutrition, 68, 1380S 1384S
43 - J. K. O’Neill, M. Sargent, M. L. Trimble, 1980 Determination of phytate in foods by phophorus 31 Fournier transfrom nuclear magnetic resonance spectrometry. Analytical Chemistry, 52, 1288 1291
44 - M. R. Ogborn, E. Nitschmann, N. Bankovic-Calic, H. A. Weiler, H. M. Aukema, 2010 Dietary soy protein benefit in experimental kidney disease is preserved after isoflavone depletion of diet. Experimental Biology and Medicine. 235(11), 1315 1320
45 - O. Omosaiye, M. Cheryan, 1979 Low-phytate, full fat soy protein product by ultrafiltration of aqueous extracts of whole soybeans. Cereal Chemistry, 56, 58 62
46 - T. Ono, 2003 Soy (soya) Cheeses In: Encyclopedia of Food Sciences and Nutrition, Second edition, Editors: Caballero B, Trugo L. C. Finglas P. M., Acadamic Press- 012227055 5398 5402
47 - A. J. Onuegbu, J. M. Olisekodiaka, M. O. Onibon, A. A. Adesiyan, C. A. Igbeneghu, 2011 Consumption of soymilk lowers atherogenic lipid fraction in healthy individuals. Journal of Medicinal Food, 14(3), 257 260
48 - H. B. Patisaul, W. Jefferson, 2010 The pros and cons of phytoestrogens. Frontiers in Neuroendocrinology, 31(4), 400 419
49 - L. M. Paucar-Menacho, M. A. Berhow, J. M. G. Mandarino, Y. K. Chang, E. G. D. Mejia, 2010a Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivar BRS 258. Food Research International, 43(7), 1856 1865
50 - L. M. Paucar-Menacho, M. A. Berhow, J. M. G. Mandarino, E. G. de Mejia, Y. K. Chang, 2010b Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology. Food Chemistry, 119(2), 636 642
51 - R. W. Peace, G. Sarwar, S. P. Touchburn, 1994 Trypsin inhibitor levels in soy-based infant formulas and commercial soy protein isolates and concentrates. Food Research International, 25 137 141
52 - S. M. Potter, J. A. Baum, H. Teng, R. J. Stillman, N. F. Shay, J. W. Erdman, 1998 Soy protein and isoflavones. American Journal of Clinical Nutrition, 68, 1375S 1379S
53 - K. R. Price, N. M. Griffiths, C. L. Curl, G. R. Fenwick, 1985 Undesirable sensory propeties of the dried pea. Food Chemistry, 25, 105 115
54 - V. Raboy, D. B. Dickinson, F. E. Below, 1984 Variation in seed total phosphorus, phytic acid, zinc, calcium, magnesium and protein among lines of Glycine max and G. soja. Crop Science, 24, 431 434
55 - G. S. Ranhotra, R. J. Loewe, L. V. Puyat, 1974 Phytic acid in soy and its hydrolysis during breadmaking. Journal of Food Science, 39, 1023 1025
56 - S. Reinwald, S. R. Akabas, C. M. Weaver, 2010 Whole versus the piecemeal approach to evaluating soy. Journal of Nutrition, 140(12), 2335S 2343S
57 - S. Reinwald, C. M. Weaver, 2010 Soy components vs. whole soy: Are we betting our bones on a long shot? Journal of Nutrition, 140(12), 2312S 2337S
58 - C. R. Rekha, G. Vijayalakshmi, 2010a Bioconversion of isoflavone glycosides to aglycones, mineral bioavailability and vitamin B complex in fermented soymilk by probiotic bacteria and yeast. Journal of Applied Microbiology, 109(4), 1198 1208
59 - C. R. Rekha, G. Vijayalakshmi, 2010bInfluence of natural coagulants on isoflavones and antioxidant activity of tofu. Journal of Food Science and Technology, 47(4), 387393
60 - C. R. Rekha, G. Vijayalakshmi, 2011 Isoflavone phytoestrogens in soymilk fermented with β-glucosidase producing probiotic lactic acid bacteria. International Journal of Food Science and Nutrition, 62(2), 111 120
61 - T. A. Russin, Y. Arcand, J. I. Boye, 2007 Particle size effect on soy protein isolate extraction. Journal of Food Processing and Preservation, 31(3), 308 319
62 - F. M. Sacks, A. Lichtenstein, L. Van Horn, W. Harris, P. Kris-Etherton, M. Winston, 2006 Soy protein, isoflavones, and cardiovascular health: An American Heart Association Science Advisory for Professionals from the Nutrition Committee. Circulation, 113, 1034 1044
63 - M. Sakač, J. Čanadanovič-Brunet, A. Mišan, V. Tumbas, D. Medič, 2010 Antioxidant activity of phytic acid in lipid model system. Food Technology and Biotechnology, 48(4), 524 529
64 - M. J. Schaefer, J. Love, 1992 Relationship between soybean components and tofu texture. Journal of Food Quality, 15, 53 66
65 - E. M. Schuster, C. E. Bodwell, 1980 Phytic acid content of thirty-two commercial soy products. Federation Proceedings, 39, 659 abs).
66 - E. Shimoni, 2004 Stability and shelf life of bioactive compound during food processing and storage : Soy isoflavones. Journal of Food Science: Concise Reviews in Food Science, 69(6), R160 R166
67 - W. Shurtleff, A. Aoyagi, 1983 The book of tofu, Protein source of the future …now!, Completed & Unabridged, vol I, Ten Speed Press- A Soyfoods Center book 0-89815-095-7
68 - W. Shurtleff, A. Aoyagi, 2000 Tofu & soymilk Production, The book of tofu, volume II, A Craft and Technical Manual, Soyfoods Center 1-92891-404-1
69 - H. E. Snyder, T. W. Know, 1987 Soybean Utilization. Van Nostrand Rienhold Company Inc, 115th Fifth Avenue New York, New York, 10003, an AVI book- 0-44228-216-6
70 - P. A. Sobral, G. G. Palazolo, J. R. Wagner, 2010 Thermal behavior of soy protein fractions depending on their preparation methods, individual interactions, and storage conditions. Journal of Agricultural and Food Chemistry, 58(18), 10092 10100
71 - P. A. Sobral, J. R. Wagner, 2009 Relationship between the composition and antitryptic activity of soy and tofu wheys and thermal behavior of their isolated protein. Informacion Tecnologica, 20(5), 65 74
72 - F. W. Souci, W. Fachman, H. Kaut, 2000 Food composition and nutrition tables, 6th revised and completed edition, Medphram Scientigfic publishers, CRC press.
73 - S. Sudarmadji, P. Markakis, 1977 The phytate and phytase of soybean tempeh. Journal of the Science of Food and Agriculture, 28, 381 383
74 - N. Sutar, P. P. Sutar, G. Singh, 2010 Evaluation of different soybean varieties for manufacture soy ice cream. International Journal of Dairy Technology, 63(1), 136 142
75 - Buckle. K. A. Sutardi, 1985 Reduction in phytic acid levels in soybeans during tempeh production, storage and frying. Journal of Food Science, 50, 260 263
76 - Buckle. K. A. Sutardi, 1985b Phytic acid changes in soybeans fermented by traditional inoculum and six strains of Rhizopus oligosporus. Journal of Applied Bacteriology, 58, 539 543
77 - K. Taku, N. Lin, D. Cai, J. Hu, X. Zhao, Y. Zhang, P. Wang, M. K. Melby, L. Hooper, M. S. Kurzer, S. Mizuno, Y. Ishimi, S. Watanabe, 2010 Effects of soy isoflavone extract supplements on blood pressure in adult humans: Systematic review and meta-analysis of randomized placebo-controlled trials. Journal of Hypertension, 28(10), 1971 1982
78 - K. Taku, K. Umegaki, Y. Ishimi, S. Watanabe, 2008 Effects of extracted soy isoflavones alone on blood total and LDL cholesterol: Meta-analysis of randomized controlled trials. Journal of Therapeutics and Clinical Risk Management, 5, 1097 1103
79 - A. M. Telang, B. N. Thorat, 2010 Optimization of process parameters for spray drying of fermented soy milk. Drying Technology, 28(12), 1445 1456
80 - J. Tian, S. Zhao, B. Zhai, L. Xu, 2010 Biotransformation of group B soybean saponins. 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE. 978-1-42444-712-1 1 3
81 - K. Toda, K. Takahashi, T. Ono, K. Kitamura, T. Nakamura, 2006 Variation in phytic acid content of soybeans and its effect on consistency of tofu made from soybean varieties with high protein content. Journal of the Science of Food and Agriculture, 86, 212 219
82 - C. Tsai, Y. Chen, Y. Chien, W. Huang, S. Lin, 2010 Effect of soy saponin on the growth of human colon cancer cells. World Journal of Gastroenterology, 16(27), 3371 3376
83 - W. B. Van der Riet, A. W. Wight, J. J. L. Cilliers, J. M. Datel, 1987 Food chemical analysis of tempeh prepared from South African-grown soybeans. Food Chemistry, 25, 197 206
84 - W. B. Van der Riet, A. W. Wight, J. J. L. Cilliers, J. M. Datel, 1989 Food chemical investigation of tofu and its byproduct okara. Food Chemistry, 34, 193 202
85 - H. L. Wang, E. W. Swain, W. F. Kwolek, 1983 Effect of soybean varieties on the yield and quality of tofu. Cereal Chemistry, 60, 245 248
86 - J. Wu, X. Ding, 2002 Characterization of inhibition and stability of soy-protein-derived angiotensin I-converting enzyme inhibitory peptides. Food Research International, 35, 367 375
87 - H. Y. T. Yang, E. G. Erdos, Y. A. Levin, 1970 A dipeptidyl carboxypeptidase that converts angiotensin I and inactivates bradykinin. Biochimica et Biophysica Acta, 214, 374 376
88 - H. Y. Yang, S. C. Yang, S. T. Chen, J. R. Chen, 2008 Soy protein hydrolysate ameliorates cardiovascular remodeling in rats with L-NAME-induced hypertension. Journal of Nutritional Biochemistry, 19, 833 839
89 - X. G. Zhuo, M. K. Melby, S. Watanabe, 2004 Soy isoflavone intake lowers serum LDL cholesterol: A meta-analysis of 8 randomized controlled trials in humans. Journal of Nutrition, 134, 2395 2400