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Biochemistry, Genetics and Molecular Biology
Gel Electrophoresis - Principles and Basics
Edited by Sameh Magdeldin, ISBN 978-953-51-0458-2, Hard cover, 346 pages, Publisher: InTech, Chapters published April 04, 2012 under CC BY 3.0 license
DOI: 10.5772/2205
Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers.
- Chapter 1
Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities - Chapter 2
Gel-Electrophoresis and Its Applications - Chapter 3
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis - Chapter 4
Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis - Chapter 5
Gel Electrophoresis of Proteins - Chapter 6
Gel Electrophoresis of Protein - From Basic Science to Practical Approach - Chapter 7
Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective - Chapter 8
High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: A Tool for Identification of Polymorphic and Modified Linker Histone Components - Chapter 9
Two-Dimensional Gel Electrophoresis (2-DE) - Chapter 10
High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis - Chapter 11
Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters - Chapter 12
Statistical Analysis of Gel Electrophoresis Data - Chapter 13
Quantitative Analysis of Electrophoresis Data - Application to Sequence-Specific Ultrasonic Cleavage of DNA - Chapter 14
The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level - Chapter 15
Electrophoretic Techniques in Microbial Ecology - Chapter 16
Application of Multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCR for Molecular Analysis of Enterococci - Chapter 17
The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Comparison with MLVA Method Coupled with Gel Electrophoresis - Chapter 18
Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting - Chapter 19
Application of Two-Dimensional Gel Electrophoresis to Microbial Systems

