About the book
Bioreactors have become an integral part of tissue engineering and regenerative medicine enterprise. In the traditional paradigm of tissue engineering, cells are added to scaffolds by seeding and then the living constructs need to be maintained in physiologic conditions of temperature, flow of nutrients and gases, and mechanical or electrical stimuli typical of the target tissues. In many instances, the living constructs need to be exposed to additional specific stimuli for adaptation and maturation. Depending on the intended application, constructs will be kept alive for extended periods to serve as in vivo testing platforms, or prepared for implantation by in vitro conditioning. During this phase, cell phenotypes need to be maintained quiescent, under ideal perfusion conditions, with manual or automatic control over all parameters. To achieve these requirements, we need to design modular systems capable of exposing the constructs to stimuli in a measurable and controllable manner. This requires specific expertise in CAD, 3D printing, sterilization, parameter controls, instrumentation, software, hardware, pneumatics, biomechanics and cell biology, among others.
This book intends to provide the reader with an overview of the requirements for bioreactor design and implementation as well as specific applications for tissue engineering and regenerative medicine.