About the book
Genetic resources represent the genetic storehouse of information, and this needs to be explored, evaluated, cataloged, and conserved so that no genotype is lost from the treasury of genome diversity. It is critical that all available methods, including cryopreservation applications, are explored. Cryopreservation is the cooling of cells to sub-zero temperatures. All metabolic systems are preserved indefinitely, which may be an illusion due to the associated difficulties in revitalizing cells after cooling. However, when successful, this method holds the future for cell preservation. This book is about research outputs, proposals, and challenges associated with using cryopreservation techniques to conserve germplasm for posterity. It considers various types of plant parts and germlines used for conservation. Successful preparation of cells and tissues prior to cooling is critical to ensure restoration after cooling, and appropriate use of cryoprotectants is important. This book also focuses on the effect of cryoprotection in cryopreservation protocol.