Open access peer-reviewed Edited Volume

Cryopreservation - Applications and Challenges

Marian Quain

Council of Scientific and Industrial Research Ghana

A pioneering researcher in plant tissue culture and related sciences in Sub-Sahara Africa. Prof. Quain has published a number of book chapters, peer-reviewed publications in local and international journals, conference papers, and laboratory manuals. In 2018 Prof. Quain received Award in Excellence in Teaching and Outreach from the American Society for Plant Biologists


Plant Genetic Resources Explant Conditioning Cryoprotection Conservation Cryoprotectants Germplasm Freezing Mode of Cooling Stem Cells Cryoprotectants Biobank Conservation

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About the book

Genetic resources represent the genetic storehouse of information, and this needs to be explored, evaluated, cataloged, and conserved so that no genotype is lost from the treasury of genome diversity. It is critical that all available methods, including cryopreservation applications, are explored. Cryopreservation is the cooling of cells to sub-zero temperatures. All metabolic systems are preserved indefinitely, which may be an illusion due to the associated difficulties in revitalizing cells after cooling. However, when successful, this method holds the future for cell preservation. This book is about research outputs, proposals, and challenges associated with using cryopreservation techniques to conserve germplasm for posterity. It considers various types of plant parts and germlines used for conservation. Successful preparation of cells and tissues prior to cooling is critical to ensure restoration after cooling, and appropriate use of cryoprotectants is important. This book also focuses on the effect of cryoprotection in cryopreservation protocol.

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Book initiated and editor appointed

Date completed: May 11th 2022

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Deadline for chapter proposals: June 8th 2022

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Deadline for full chapters: August 7th 2022

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Review results due: October 26th 2022

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Expected publication date: December 25th 2022

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About the editor

Marian Quain

Council of Scientific and Industrial Research Ghana

Prof. Marian D. Quain is a Principal Research Scientist (Biotechnologist) at CSIR – Crops Research Institute Kumasi, as well an Associate Professor at CSIR – Graduate School CCST Prof. Quain holds a B.Sc. (Hons.) Degree in Botany with Zoology, M.Phil (Botany – Plant Physiology) and Ph.D. (Botany-Plant Physiology) from the University of Ghana, Legon. Her research interest is based on Biotechnology. She has expertise in tissue culture, cryopreservation, genetic transformation, plant physiology, molecular biology, aeroponics, Biosafety, and Biotechnology stewardship. Prof. Quain research focus is on the utilization of tissue culture for the production of clean planting materials of vegetatively propagated crops, germplasm conservation and the use of molecular tools for crop diversity, disease diagnostics, and molecular marker-assisted selection to facilitate current state-of-the-art breeding using transcriptomics and proteomics, as well as application of recombinant gene technology for crop improvement. Prof. Quain joined the CSIR-Crops research institute in 1996 and initiated tissue culture research activities. During her Ph.D. studies (2002-2007) she carried out certain aspects of the research at the University of Kwa-Zulu-Natal Howard Campus in Durban South Africa as well as the Tuskegee University Alabama USA as a fellow of the Third World Organization for Women Science. Under the Schlumberger Foundation Fellowship for Future Faculty, Marian was a Visiting Research Fellow at the Leeds University UK (2011-2013). Prof. Quain has published a number of book chapters, peer-reviewed publications in local and international journals, conference papers and laboratory manuals. She contributed with advice and expertise in many Tissue culture and molecular biology laboratories in Ghana and the West African Sub-region. She has also trained technician and researchers, both locally and internationally, on the application of Biotechnology. Currently, Prof. Quain is introducing high school teachers in Ghana to biotechnology. In 2013, she was awarded for the Best Research Scientist by the Ministry of Food and Agriculture in connection with the Farmers Day Celebrations in Ghana. She was a 2017 Cochran Fellow and has also completed (2017 – 2018) Africa Science leadership Program Fellowship. In 2018 Prof. Quain received Award in Excellence in Teaching and Outreach from the American Society for Plant Biologists. She served as Deputy Director for the CSIR - Crops Research Institute from October 2019 to December 2021. She leads research in seed systems of vegetatively propagated crops in her institute.

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