About the book
The book will describe gel electrophoresis and it’s applications. First topic will be dedicated to the principles of electrophoresis (voltage and ionic strength of buffers will also be addressed). Second topic will be on the DNA gel electrophoresis. Among other things it will address staining of gel for visualizing the DNA bands, different concentrations of agarose gel for separation of DNA of different sizes, DNA marker (used for estimation the rough molecular weight of DNA sample) etc.Third topic will be on protein gel electrophoresis. Along with other things it will address the percentages of polyacrylamide gel for separation of protein on the gel, staining of the polyacrylamide gel with Commassie Brilliant Blue G250 or R250 or silver staining. Fourth topic will cover protocol used for the working of gel electrophoresis apparatus, preparation of agarose gel and buffers. Fifth topic will cover troubleshooting with gel electrophoresis (i.e. bad resolution, heating up of apparatus, voltage problems etc.). Sixth topic will be on different companies manufacturing the gel electrophoresis apparatus.