Open access peer-reviewed Edited Volume

Electrophoresis

Farheen Aslam

Lahore College for Women University

Covering

Voltage Ionic Concentration Size of Agarose Gel Buffers Staining of Gel Ethidium Bromide SDS-PAGE Protein Marker Agarose Gel Positive and Negative Electrode Bad Resolution Voltage Problem

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About the book

The book will describe gel electrophoresis and it’s applications. First topic will be dedicated to the principles of electrophoresis (voltage and ionic strength of buffers will also be addressed). Second topic will be on the DNA gel electrophoresis. Among other things it will address staining of gel for visualizing the DNA bands, different concentrations of agarose gel for separation of DNA of different sizes, DNA marker (used for estimation the rough molecular weight of DNA sample) etc.Third topic will be on protein gel electrophoresis. Along with other things it will address the percentages of polyacrylamide gel for separation of protein on the gel, staining of the polyacrylamide gel with Commassie Brilliant Blue G250 or R250 or silver staining. Fourth topic will cover protocol used for the working of gel electrophoresis apparatus, preparation of agarose gel and buffers. Fifth topic will cover troubleshooting with gel electrophoresis (i.e. bad resolution, heating up of apparatus, voltage problems etc.). Sixth topic will be on different companies manufacturing the gel electrophoresis apparatus.

Publishing process

Book initiated and editor appointed

Date completed: October 18th 2019

Applications to edit the book are assessed and a suitable editor is selected, at which point the process begins.

Chapter proposals submitted and reviewed

Deadline Extended: Open for Submissions

Potential authors submit chapter proposals ready for review by the academic editor and our publishing review team.

Approved chapters written in full and submitted

Deadline for full chapters: May 1st 2020

Once approved by the academic editor and publishing review team, chapters are written and submitted according to pre-agreed parameters

Full chapters peer reviewed

Review results due: July 20th 2020

Full chapter manuscripts are screened for plagiarism and undergo a Main Editor Peer Review. Results are sent to authors within 30 days of submission, with suggestions for rounds of revisions.

Book compiled, published and promoted

Expected publication date: September 18th 2020

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About the editor

Farheen Aslam

Lahore College for Women University

Farheen Aslam MS, PhD, is Assistant Professor at Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan. She has obtained her Ph.D. from School of Biological Sciences, University of the Punjab, Pakistan in 2012: her research topic was “Cloning. expression and physicochemical analysis of proinsulin and its derivatives”. Her research field includes human biochemistry and disease, molecular biology and recombinant DNA technology, protein expression and purification, refolding of protein, enzymology, genetics and microbiology. She is proficient in use of HPLC system. MALDI-TOF, Polymerase chain reaction (PCR), Agarose Gel Electrophoresis, UV-Spectrophotometer, RNA, Plasmid and Genomic DNA Isolation. She worked as a Research Officer in the Mass Spectrometry Lab of School of Biological Sciences, University of the Punjab, Lahore from 23rd August 2010 to 23rd November 2010. Responsibilities were to prepare and analyze the samples on Voyager De Pro (ABI) MALDI TOF mass spectrometry workstation. She was awarded with a Startup Research Grant on “Studying the effect on expression of a recombinant protein by changing the nucleotides at the promoter & start of the gene” of PKR 0.5 million by Higher Education Commission on June 4th, 2013. She has participated in 19 conferences at National and international level. She has Published 11 research paper in National and International journals.

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As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. Whether that be identifying an exceptional author and proposing an editorship collaboration, or contacting researchers who would like the opportunity to work with IntechOpen, I establish and help manage author and editor acquisition and contact.

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