About the book
The in-depth understanding of biological species requires a concerted use of various investigation modalities capable of providing valuable and quantitative information on their structure and function. In such efforts, optical techniques play a tremendous role, as they can be ultrafast, non-invasive, and capable of spatial resolutions ranging from macro- to nanoscale. They allow imaging molecules, cells and tissues with high specificity in-vitro, ex-vivo and in-vivo, which is useful not only for fundamental research but also for other important purposes such as diagnostics. Combining complementary optical techniques relying on distinct contrast-mechanisms and operating at different scales represents nowadays the default approach in most experiments aimed at shedding light on still unresolved aspects of key topics such as cell life and fate, or start and progression of pathologies. The potential of such multimodal frameworks can be augmented by means of advanced image analysis tools, which can accelerate research outputs with orders of magnitude and also extract information unavailable to a human expert due to its level of complexity and/or scale. This book intends to provide the reader extensive knowledge on: (i) recent tools with great utility in multimodal optical bioimaging, (ii) advanced protocols for sample preparation methods that enhance bioimaging performance, (iii) important life science applications that exploit the power of complementary optical techniques to resolve aspects difficult or impossible to tackle otherwise, and (iv) latest image analysis methods and workflows capable to extract valuable information from multimodal data sets in a fast and precise way.