About the book
Cell culture refers to an in vitro method that simulates in vivo environment such as asepsis, appropriate temperature, pH and certain nutritional conditions to enable cell to survive, grow, reproduce and maintain its structure and function. Cell culture is actually cell cloning technology to large-scale clone cells to study cell signal transduction, cell anabolism, cell growth and proliferation. It is an important and common technology in cell biology and bioengineering research. Cell culture includes human cell culture, animal cell culture, plant cell culture, and microbial cell culture according to the cell origin. Also, cell culture includes primary culture, secondary culture, and cell co-culture per the experiment requirements. Different types of cell culture have its own characteristics and essential conditions. However, one must realize that cell culture is an in vitro method, which cannot replace in vivo animal experiment and human trials. Generally, the results derived from in vitro cell experiments must be verified by in vivo animal experiment, and further by human trials.
This book will focus on the new opinion, methods, and advance of cell culture (including primary culture, secondary culture, and cell co-culture), assessment of experimental results derived from cultured cells, and application of cell culture in life science and medical science.