Open access peer-reviewed Edited Volume

Cell Culture

Xianquan Zhan

Shandong First Medical University

A pioneering expert in the field of proteomics and proteoforms, multi-omics, biomarkers, and 3P medicine in cancer, appointed as Fellow of Royal Society of Medicine, Fellow and National Representative of EPMA, and Associate Editor of EPMA Journal and BMC Medical Genomics.


Human Cell Animal Cell Plant Cell Culture Microbial Cell Culture Primary Culture Secondary Culture Cell Co-culture Medium pH Value Antibiotics Cell Culture Procedure Application of Cell Culture

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About the book

Cell culture refers to an in vitro method that simulates in vivo environment such as asepsis, appropriate temperature, pH and certain nutritional conditions to enable cell to survive, grow, reproduce and maintain its structure and function. Cell culture is actually cell cloning technology to large-scale clone cells to study cell signal transduction, cell anabolism, cell growth and proliferation. It is an important and common technology in cell biology and bioengineering research. Cell culture includes human cell culture, animal cell culture, plant cell culture, and microbial cell culture according to the cell origin. Also, cell culture includes primary culture, secondary culture, and cell co-culture per the experiment requirements. Different types of cell culture have its own characteristics and essential conditions. However, one must realize that cell culture is an in vitro method, which cannot replace in vivo animal experiment and human trials. Generally, the results derived from in vitro cell experiments must be verified by in vivo animal experiment, and further by human trials.

This book will focus on the new opinion, methods, and advance of cell culture (including primary culture, secondary culture, and cell co-culture), assessment of experimental results derived from cultured cells, and application of cell culture in life science and medical science.

Publishing process

Book initiated and editor appointed

Date completed: February 4th 2021

Applications to edit the book are assessed and a suitable editor is selected, at which point the process begins.

Chapter proposals submitted and reviewed

Deadline for chapter proposals: March 4th 2021

Potential authors submit chapter proposals ready for review by the academic editor and our publishing review team.

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Approved chapters written in full and submitted

Deadline for full chapters: May 3rd 2021

Once approved by the academic editor and publishing review team, chapters are written and submitted according to pre-agreed parameters

Full chapters peer reviewed

Review results due: July 22nd 2021

Full chapter manuscripts are screened for plagiarism and undergo a Main Editor Peer Review. Results are sent to authors within 30 days of submission, with suggestions for rounds of revisions.

Book compiled, published and promoted

Expected publication date: September 20th 2021

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About the editor

Xianquan Zhan

Shandong First Medical University

Xianquan Zhan received his M.D. and Ph.D. training in preventive medicine at the West China University of Medical Sciences from 1989 to 1999. He received his post-doctoral training in oncology and cancer proteomics at the Central South University and University of Tennessee Health Science Center (UTHSC). He worked at UTHSC and Cleveland Clinic in USA from 2001 to 2012, and achieved the rank of Associate Professor at UTHSC. After that, he became a Full Professor at the Central South University and Shandong First Medical University, and an Advisor of MS/PhD graduate students and postdoctoral fellows. He is also a Fellow of the Royal Society of Medicine, Fellow of EPMA, European EPMA National Representative, full member of the American Society of Clinical Oncology (ASCO), member of the American Association for the Advancement of Sciences (AAAS), Editor-In-Chief of the International Journal of Chronic Diseases & Therapy, Associate Editor of the EPMA Journal and BMC Medical Genomics, and Guest Editor of Frontiers in Endocrinology and Mass Spectrometry Reviews. He has published 135 articles, 23 book chapters and 2 US patents in the field of clinical proteomics and biomarkers. He edited 4 academic books: Proteoforms – Concept and Applications in Medical Sciences, Molecular Network Study of Pituitary Adenomas, and Ubiquitin-Proteasome Pathway. As the Guest editor, he edited 11 special issues in SCI journals such as Mass Spectrometry Reviews, Frontiers in Endocrinology, EPMA Journal, and Med One. His main research interest focuses on the studies of cancer proteomics and proteoforms, multiomics and biomarkers, and the use of modern omics techniques and systems biology for predictive, preventive and personalized medicine (PPPM) and precision medicine (PM) in cancer.

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Book chapters authored 8

Books edited 2

Introducing your Author Service Manager

Ms. Mia Miskulin

As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. Whether that be identifying an exceptional author and proposing an editorship collaboration, or contacting researchers who would like the opportunity to work with IntechOpen, I establish and help manage author and editor acquisition and contact.

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