About the book
Cryopreservation is the storage of biological material at ultra-low temperature of liquid nitrogen, which arrests all metabolic activities. Theoretically, no genetic changes should occur and this, therefore, permits indefinite cell preservation. There have been several limitations to the application of the techniques, although substantial successes have been reported for both animal and plant cells. During cryopreservation, several factors come into play- cell type, size, and metabolic state.
Cells have to be protected from damage especially by ice crystals during freezing. Cryoprotectants are widely used, however, these may be toxic to the cells. Mode of freezing-thawing and regeneration also may render the process successful. Cell viability may be lost during any step of the process. The main objective of this book is to examine factors that affect cells during cryopreservation process which can contribute towards the successful application of this important technique.