Open access peer-reviewed Edited Volume


Marian Quain

Council for Scientific and Industrial Research


Cryopreservation Oocyte cryopreservation Embryo cryopreservation Lateral and Axillary Buds Zygotic and Somatic Embryos Recalcitrant Seeds Cryopreservation Formulation of Vitrification solution Reactive Oxygen Species (ROS) Cryoprotective Agents (CPA) Freezing Mechanisms

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About the book

Cryopreservation is the storage of biological material at ultra-low temperature of liquid nitrogen, which arrests all metabolic activities. Theoretically, no genetic changes should occur and this, therefore, permits indefinite cell preservation. There have been several limitations to the application of the techniques, although substantial successes have been reported for both animal and plant cells. During cryopreservation, several factors come into play- cell type, size, and metabolic state.
Cells have to be protected from damage especially by ice crystals during freezing. Cryoprotectants are widely used, however, these may be toxic to the cells. Mode of freezing-thawing and regeneration also may render the process successful. Cell viability may be lost during any step of the process. The main objective of this book is to examine factors that affect cells during cryopreservation process which can contribute towards the successful application of this important technique. 

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Book initiated and editor appointed

Date completed: August 8th 2019

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Deadline for chapter proposals: August 29th 2019

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Review results due: January 16th 2020

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Expected publication date: March 16th 2020

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About the editor

Marian Quain

Council for Scientific and Industrial Research

Prof. Marian D. Quain is a Principal Research Scientist (Biotechnologist) at CSIR – Crops Research Institute Kumasi, as well an Associate Professor at CSIR – Graduate School CCST Prof. Quain holds a B.Sc. (Hons.) Degree in Botany with Zoology, M.Phil (Botany – Plant Physiology) and Ph.D. (Botany-Plant Physiology) from the University of Ghana, Legon. Her research interest is based on Biotechnology. She has expertise in tissue culture, cryopreservation, genetic transformation, plant physiology, molecular biology, aeroponics, Biosafety, and Biotechnology stewardship. Prof. Quain research focus is on the utilization of tissue culture for the production of clean planting materials of vegetatively propagated crops, germplasm conservation and the use of molecular tools for crop diversity, disease diagnostics, and molecular marker-assisted selection to facilitate current state-of-the-art breeding using transcriptomics and proteomics, as well as application of recombinant gene technology for crop improvement. Prof. Quain joined the CSIR-Crops research institute in 1996 and initiated tissue culture research activities. During her Ph.D. studies (2002-2007) she carried out certain aspects of the research at the University of Kwa-Zulu-Natal Howard Campus in Durban South Africa as well as the Tuskegee University Alabama USA as a fellow of the Third World Organization for Women Science. Under the Schlumberger Foundation Fellowship for Future Faculty, Marian was a Visiting Research Fellow at the Leeds University UK (2011-2013). Prof. Quain has published a number of book chapters, peer-reviewed publications in local and international journals, conference papers and laboratory manuals. She contributed with advice and expertise in many Tissue culture and molecular biology laboratories in Ghana and the West African Sub-region. She has also trained technician and researchers, both locally and internationally, on the application of Biotechnology. Currently, Prof. Quain is introducing high school teachers in Ghana to biotechnology. In 2013, she was awarded for the Best Research Scientist by the Ministry of Food and Agriculture in connection with the Farmers Day Celebrations in Ghana. She was a 2017 Cochran Fellow and has also completed (2017 – 2018) Africa Science leadership Program Fellowship. In 2018 Prof. Quain received Award in Excellence in Teaching and Outreach from the American Society for Plant Biologists.

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