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Jarzyna, Maria Bała, Paulina I. Krakowska, Edyta\nPuskarczyk, Anna Strzępowicz, Kamila Wawrzyniak-Guz, Dariusz\nWięcław and Jerzy Ziętek",authors:[{id:"179925",title:"Prof.",name:"Jadwiga",middleName:null,surname:"Jarzyna",fullName:"Jadwiga Jarzyna",slug:"jadwiga-jarzyna"},{id:"186797",title:"Prof.",name:"Maria",middleName:null,surname:"Bała",fullName:"Maria Bała",slug:"maria-bala"}]},{id:"56285",title:"An Overview of Principles and Designs of Hydraulic Fracturing Experiments and an Inquiry into the Influence of Rock Permeability and Strength on Failure Mode",slug:"an-overview-of-principles-and-designs-of-hydraulic-fracturing-experiments-and-an-inquiry-into-the-in",signatures:"Kenneth Imo‐Imo Eshiet and Yong Sheng",authors:[{id:"195037",title:"Dr.",name:"Kenneth Imo-Imo",middleName:null,surname:"Eshiet",fullName:"Kenneth Imo-Imo Eshiet",slug:"kenneth-imo-imo-eshiet"}]}]}]},onlineFirst:{chapter:{type:"chapter",id:"57558",title:"Influence of Selected Per Orally Administered ATB on Microflora of GIT in Experimental Animals",doi:"10.5772/intechopen.71554",slug:"influence-of-selected-per-orally-administered-atb-on-microflora-of-git-in-experimental-animals",body:'Although the use of antibiotics administered antibiotics (ATB) is nowadays often necessary, there is still a number of issues that arise from their abuse. It is known, that excessive use of ATB has a negative impact on physiological composition of intestinal microbiota, especially when they are administered per os. This is due to increase in gastrointestinal (GIT) diseases. To understand the impact of ATB on GIT microbiota it is necessary to know the correct composition of the GIT microbiota and changes induced by various ATB in this convocation. The most common pattern for tracking changes in the microflora is faeces. However, there is little knowledge on microbiological changes in various parts of GIT. Experimental animals, both conventional and gnotobiotic, were used in relevant studies. However, they were fed a different type of food in addition to a number of anatomical and physiological differences. Therefore, for many scientists this issue still remains a great mystery. Also, until the development of sensitive molecular methods, conventional culture methods were used to track these changes. However, since 40–90% of the intestinal bacteria are not cultivable, scientists looked for and tested more sensitive and accurate methods for the detection and quantification of microorganisms [1]. For example, developed were methods based on PCR-DGGE, real-time PCR, and others. However, even these methods have shortcomings that require an amplification process which may introduce an untargeted error. The fluorescent-in-situ-hybridization (FISH) method is independent of the amplification and is sufficiently sensitive to trap even non-cultivable microorganisms. So far scientists have used a number of FISH to determine the physiological composition of microbiota of GIT, either animal or human. In addition, the new development allows one to monitor potential changes under the impact of substances added to the diet in both experimental animals and clinical patients. The aim of this study was to summarise the findings on the impact of ATB on composition of intestinal microbiota by means of FISH method using available sources and compare them with previously published knowledge in this area. The importance of this study consists in finding out whether it is possible to track by this method the changes in GIT microbiota produced by ATB and thus contribute to the body of knowledge in this area.
Recently, the increasing resistance of bacterial agents to ATBs, such as Methicillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas, stressed the importance of the development of novel ATB derivatives [2]. New classes of antibiotics are urgently needed to treat nosocomial infections. The risk of increasing ATB resistance also increases due to the increased use of broad-spectrum ATBs in unprofessional human and veterinary clinical practice, without detecting the bacterial origin of the disease and its sensitivity to ATBs. ATB residues in food of animal origin from countries not complying with the 2006 EU Directive have an impact on the increased risk of spreading antibiotic resistance. Development of ATB-resistant strains can be prevented by using correct therapeutic dose of ATB and completing the prescribed course of treatment. Properly balanced intestinal microflora prevents the development of resistant microbial strains. Normal microbiota acts as a barrier against the colonisation of potentially pathogenic microorganisms and against the excessive growth of opportunistic microorganisms already present. Administration of ATBs either therapeutically or as a prophylactic measure disturbs the ecological balance between the host and the normal microbiota. The clinically most common symptoms of intestinal microbiota disruption are diarrhoea and fungal infections that usually resolve after the treatment has ended [3]. It is difficult to assess the long-term consequences of microbial symbiosis disorders in the intestine. In addition to changes in intestinal microbiota, many chronic diseases such as asthma and atopic diseases are associated with the use of ATB in childhood [4].
In 1928, Alexander Fleming discovered that the growth of Penicillium notatum suppressed the growth of staphylococci, and then, as this phenomenon was studied, it was found that the cause was an exoproduct of a mould called penicillin that was released into the cultivation medium. In 1938, Howard Florey and Ernst Chain began experimenting with penicillin mould. By 1941, a sufficiently purified form of penicillin was obtained and by early 1942, American pharmaceutical companies were mass producing penicillin for distribution to Allied soldiers during the Second World War [5]. Since the first effects of ATB have been discovered, other substances with ATB properties have appeared and many have found a wide range of applications in medicine for the treatment of infections caused by bacteria, pathogenic fungi, mycoplasmas, rickets, chlamydia and some other agents [6]. Attempts to influence GIT microbiota with ATB date back to the very beginning of their use. The impact of ATB was observed in clinical practice as well as during preoperative patient preparation. With regard to animal production, it raised interest particularly for economic reasons, as it was shown that ATBs accelerate the growth and weight gain in mice, dogs, but also in pigs and calves. Experiments on germ-free chickens revealed that the nutritional effect of ATB is mainly related to suppression of some subclinical infections [7].
Antibiotics are substances of organic origin produced by bacteria and moulds, possibly from higher plants or animal tissues, and can be prepared synthetically or semi-synthetically [8]. Their name was derived from the phenotype of Pasteur, which was described by Pasteur in the 1960s.
According to their biological effect on microorganisms they are divided to two groups, one with bacteriostatic action and another one with bactericidal effect. Bacteriostatic ATBs arrest multiplication of bacteria so the bacteria are not killed and natural dying of quiescent bacterial cells is not affected. Bactericidal effect of ATBs results in death of bacterial cells. The bactericidal effect during the first 4 hours of action of ATBs is of specific importance. If at least 99% of bacteria is killed within this time we can speak about clinically relevant bactericidal action.
ATBs are divided into 5 groups according to the mechanism of action:
Inhibition of cell wall synthesis (bactericidal effect), (typical of penicillins, vancomycin, cycloserin)
Effect of cell wall function (bactericidal effect), (typical of polymyxins)
Inhibition of protein synthesis (bacteriostatic and bactericidal effect) (chloramphenicol, tetracyclines, aminoglycosides, macrolide ATBs)
Inhibition of nucleic acid synthesis (bactericidal effect), (griziofulvin, rifampicin)
Interference in the intermediary metabolism of bacteria (sulfonamides)
Not all ATBs can be administered orally, but ATBs capable of influencing GIT microbiota must be available in the form suitable for oral administration. The most commonly used orally administered ATBs include: penicillins, cephalosporins, tetracyclines, polypeptide ATBs, aminoglycosides, macrolides, Lincosamide ATB, ansamycin ATB, diterpenes, aminocoumarin ATBs, steroid antibiotics, sulfonamides and quinolones. Among these, we include the following representatives:
Penicillins:
Phenoxypenicillins: Phenoxymethylpenicillin—Penicillin V, Penamecillin, Penetacillin, Benetaminpenicillin, Phenticillin, Propicillin, Phenbenicillin, Klometocillin
Wide spectrum of penicillins:
Aminopenicillins: Ampicillin, Bakampicillin, Pivampicillin, Talampicillin, Amoxicillin, Epicillin, Cyclaclin
Carboxypenicillins: Carbenicillin Esters: Indanyl Carbenicillin, Carfecili
Amidopenicillins: Mecilinam esters: Bakmecilinam, Pivmecilinam
Isoxazolylpenicillins: Oxacillin, Dicloxacillin, Kloxacillin, Flucloxacillin, Pirazocillin
Cephalosporins: Cefalexin, Cefadroxil, Cefixim, Metacyclin, Tiacycline
Amphenicols: Chloramphenicol, Tiamfenicol, Florfenicol
Tetracyclines: Chlortetracycline, Oxytetracycline, Tetracycline, Doxycycline, Minocycline,
Polypeptide antibiotics: Polymyxins: Polymyxin B
Aminoglycosides: Streptomycin, Neomycin, Kanamycin, Apramycin, Gentamicin, Tobramycin and Aminocyclitols: Spectinomycin
Macrolides: Erythromycin, Spiramycin, Tylozine, Oleandromycin, Troleandromycin, Josamycin, Tilmicosine, Clarithromycin, Roxithromycin and Azalides: Azithromycin
Linkozamide antibiotics: Linkomycin, Klindymycin
Ansamycin antibiotics: Rifampicins: Rifampicin, Rifaximin, Rifabutin, Rifapentin
Diterpenes: Tiamulin, Valnemulin
Aminocoumarin antibiotics: Novobiocin
Antibiotics with steroid structure: Fusidic acid
According to some authors, other peroral drugs with antibacterial activity are considered antibiotics:
Other antimicrobials
Nitroimidazole derivatives: Metronidazole, Tinidazole Nimorazole
Sulfonamides:
Short-acting: Sulfathiazole, Sulfacetamide, Sulfisoxazole.
Medium-effective sulphonamides: Sulfadimidine, Sulfadiazine, Sulfamerazine, Sulfamethoxazole + Trimetoprim = Kotrixomazole, Sulfachloropyridazine.
Long-term effective: Sulfamethoxypyridazine, Sulfadoxine, Sulfadimetoxin Enteric-acting sulfonamides: Phthalylsulfathiazole, Succinylsulfathiazole, Sulfachinoxaline, Sulfaclozine
Quinolones: Nalidixic acid, Flumequin, Enrofloxacin, Difloxacin, Ciprofloxacin, Marbofloxacin, Norfloxacin, Sarafloxacin, Pefloxacin, Ofloxacin, Ibafloxacin, Orbifloxacin
Search method: we searched the PubMed, and Elsevier databases and Slovak scientific literature for the studies dealing with the effect of ATBs on GIT composition. We searched for publications in the period from 1980 to 2016 using keywords related to ATB, Influence, microbiota, FISH. A literature review was produced aimed to identify association between peroral administration of ATB to humans or animals and its effect on composition of normal microbiota in GIT.
Administration of ATBs can seriously disturb the balance of the intestinal microbiota in terms of multiplication of bacteria and development of resistant microorganisms. This can lead to infections and to the transfer of resistance factors between bacteria [9]. According to the majority of authors, the effect of ATB on nutrition is mediated by intestinal microbiota. Antibiotics are divided according to their effect on GI microbiota to ATBs capable of absorption across the intestinal wall and to those that cannot be absorbed at all or only in very small amounts. The lower the bioavailability of ATB the more it remains in the colon and thus the risk of suppression of intestinal microflora increases. If ATBs are absorbable (e.g. tetracycline, penicillin, chloramphenicol, etc.), their concentration is lower in the GIT endpoints. In contrast, ATBs incapable of absorption (e.g. streptomycin, polymyxin, neomycin, etc.) may have a strong toxic effect on the microbiota throughout the GIT. The effect of ATB is generally dependent on the dose, the active substance, the duration of administration and other factors. The search results clearly demonstrated that the effect of ATB on the GIT microbiota is as follows:
Breach of microbial balance (in GIT, urinary tract, reproduction tract, etc.).
Vitamin K hypovitaminosis as result of long-term use ATB (especially p.o.)
Resistance of resistant strains, superinfection: Candida, Staphylococcus, Pseudomonas, Clostridium difficile and others.
Evidence of rapid bacteriolysis, particularly Gram-negative bacteria (endotoxin release)
The studies of the effect of ATB on microbiological-clinical microbiota date back more than 50 years ago [10, 11]. The effect on microbiota was investigated with regard to the weight gains of conventional experimental animals. Studies on germ-free animals (without GIT microbes) showed weight gains related to ATB [11]. It is still an up-to-date topic as indicated by recent studies [12]. To demonstrate the presence of bacteria and changes in their numbers, whether under the influence of antibiotics and other substances, conventional cultivation methods are still used. However, these methods have recently been supplemented by more sensitive molecular methods. One of the methods used for quantification of bacterial population is the fluorescent-in-situ-hybridization method (FISH). These methods can be used to accurately identify and quantify the species representation of microorganisms [13]. While radioactive labelling was previously used in the FISH methodology, today we use fluorochrome-labelled probes [14]. The probes serve to specifically bind to that part of the target sequence that exhibits a high degree of sequence complementarity. The probes consist mostly of 15–30 nucleotides and are covalently labelled with a fluorescent dye at the 5 ‘end—fluorescein, tetramethylrodamine, Texas red, carbocyanine. Up to now, several probes have been standardised, which are currently used to quantify the major intestinal bacteria (Table 1). For example, a probe called (S-G-Lab-0158-a-A-20) or abbreviated Lab158 is designed to detect the presence of Lactobacillus spp./Enterococcus spp. in the monitored samples. It is an oligonucleotide with the sequence 5’X-GGT AAT AGC A (T/C) C TGT TTC-3 ‘wherein X is fluorochrome [16]. This method is particularly useful in the study of the effect of probiotics, which are often required to identify probiotic bacteria of the commensal microflora [17]. Recently it was reported that the simultaneous use of ATB and supportive probiotic therapy, which can help to restore intestinal microbiota, can also expand the antibiotic resistance of bacterial intestinal bacteria [18, 19].
Short name | Full name | Target microorganism | Sequences (5′ - 3′) |
---|---|---|---|
Sal 303 | L-S-Sal-1717-a-A-18 | Salmonella spp. | AATCACTTCACCTACGTG |
Bif164 | S-G-Bif-0164-a-A-18 | Bifidobacterium spp., Parascardovia denticolens | CATCCGGCATTACCACCC |
Lab158 | S-G-Lab-0158-a-A-20 | Lactobacillus, Weissella spp.; Lactococcus lactis; Vagococcus, Enterococcus, Melisococcus, Tetragenococcus, Catellicoccus, Pediococcus a Paralactobacillus spp. | GGTATTAGCAYCTGTTTCCA |
Bac303 | S-Bacto-0303-a-A-17 | Bacteroides sensu stricto, Prevotella spp., Parabacteroides; Barnesiella viscericola a Odoribacter splanchnicus | CCAATGTGGGGGACCTT |
Chis150 | S-Chis-0150-a-A-23 | Clostridium tyrobutyricum; Adhaeribacter aquaticus, Flexibacter canadensis, Flexibacteriaceae;Propionibacteriaceae | TTATGCGGTATTAATCTYCCTTT |
Rbro730 | S-Rbro-730-a-A-18 | Ruminococcus bromii-like; Clostridium sporosphaeroides a Clostridium leptum | TAAAGCCCAGYAGGCCGC |
Rfla729 | S-Rfla-729-a-A-18 | Ruminococcus albus a Ruminococcus flavefaciens | AAAGCCCAGTAAGCCGCC |
Ato291 | S-Ato-0291-a-A-17 | Atopobium, Colinsella, OlsenellaEggerthella spp.; Cryptobacterium curtum; Mycoplasma equigenitalium Mycoplasma elephantis | GGTCGGTCTCTCAACCC |
Erec482 | S-Erec-0482-a-A-19 | Clostridium saccharolyticum,Syntrophococcus sucromutans, Bacteroides galacturonicus Bacteroides xylanolyticus Lachnospira pectinschiza | GCTTCTTAGTCARGTACCG |
Probes for FISH analysis used to detect bacterial populations in samples from in vitro fermentation.
Source:
Table processed by the author from the original Table [15].
In addition to scientific papers dealing with the impact of antibiotics on the microflora of GIT by means of conventional culture methods, studies using FISH method were also published focusing mainly on quantification of bacterial representatives in samples of various origin. This later led to the use of this method also for the purpose of monitoring the effect of ATB on the GIT microflora not only in humans [20] but also in experimental animals that were used to determine changes in composition of microbiota. For example, using of FISH for research of effect of amoxicillin potentiated by clavulanic acid on human faecal microflora in germ-free mice [21]. To provide more clear overview, the sources obtained by search were divided on the basis of their ability to absorb across the GIT.
The results of studies dealing with the effect of amoxicillin on the microbiota indicate that per os administration caused a significant decrease in the number of total faecal bacteria by almost 30%, as determined by the universal Eub338 probe. Major microbiota populations such as Fusobacterium, Eubacterium and Atopobium were affected by amoxicillin. There was observed also a percentage increase in Bacteroides and Bifidobacterium. The results also showed that not all evaluated populations were affected by the ATB. The greatest change was observed in E. coli counts, which increased significantly during ATB administration [3, 22]. By using FISH, the effect of amoxicillin potentiated by clavulanic acid on human faecal microbiota in germ-free mice was observed [21]. In this study, amoxicillin with clavulanic acid was administered orally for 7 days and the results were compared with the control group of mice not treated with ATB. Molecular analysis of digestive microbiota was performed in a 2-week experiment using FISH in combination with flow cytometry (FC) using specific 16S rRNA target probes for Bacteroides-Porphyromonas-Prevotella, Clostridium coccoides-Eubacterium rectale, Clostridium histolyticum, Faecalibacterium prausnitzii, Enterobacteriaceae, Lactobacillus, Enterococcus, and Bifidobacterium. Clostridium coccoides-Eubacterium rectale and Bacteroides-Porphyromonas-Prevotella, which represented the dominant flora, were found to be the most abundant bacterial groups. The Clostridium coccoides group was stable in control mice (from 40.7 ± 1.6% to 45.6 ± 2.8%) but significantly decreased in the treated mice on the second day of treatment and remained at a low level throughout the ATB treatment (3.9 ± 0.8%). At the end of ATB administration, the levels increased (17.7 ± 4.7%) and by day 14 reached 36 ± 1.8%. The Bacteroides-Porphyromonas-Prevotella group in control mice persisted at 35.9 ± 4.3%, whereas in treated mice it increased from 1 to 6 days when it reached 58.5 ± 0.4.5%. From day 9, the level decreased to 38.6 ± 5.7% until it reached the same level as in control mice at the end of the experiment [21]. This animal model allowed the authors to conclude that amoxicillin potentiated by clavulanic acid disrupts the balance of the dominant anaerobic microflora and that the Clostridium coccoides group is very susceptible to amoxicillin potentiated by clavulanic acid. No Enterobacteriaceae bacteria were detected in control mice, on the other hand their number increased and they were detectable in the treated mice from day 2 of administration of ATB. From day 8, their counts decreased and from day 11 until the end of the experiment they were no more detectable. Faecalibacterium prausnitzii and Clostridium histolyticum were present in 1.3 ± 2.1% and 0.4 ± 0.4% of control mice [21]. No bacteria were detected in the treated mice during administration of ATB, i.e. these bacterial groups were sensitive to amoxicillin-clavulanic acid. From day 1 to day 14 after administration of ATB, the counts of these groups of bacteria were similar to those in control mice. The probes for Bifidobacterium, Lactobacillus and Enterococcus did not detect any signals in either treated or control mice [21]. During 7 days of per os treatment with amoxicillin potentiated with clavulanic acid, the effect of Saccharomyces boulardii yeasts on the composition of intestinal microbiota in mice associated with human microbiota was also investigated. The predominant groups of bacteria were quantified by FISH in combination with flow cytometry. Probes for Eubacteria, Bacteroides-Porphyromonas-Prevotella, Clostridium coccoides-Eubacterium rectale, Faecalibacterium prausnitzii, Clostridium histolyticum, Lactobacillus-Enterococcus, Enterobacteriaceae and Bifidobacterium species have been used. The observed mice were divided into two groups of mice, the first group received yeast and the second did not. In the second group the level of Enterobacteriaceae and Bacteroides increased but the numbers of C. coccoides-E rectale dropped dramatically. After ATB treatment, the original intestinal flora was restored more rapidly for C. coccoides-E. rectale and Bacteroides-Porphyromonas-Prevotella in S. boulardii mice versus control mice (p < 0.05) [21]. The effect of other beta lactam ATBs on the microbiota, in particular of imipenem, was also observed using the FISH method (Dubourg et al., [23]). The susceptibility of Akkermansia muciniphila with respect to the effect of imipenem was also studied. In this case, the FISH method utilised a specific protozoan 5 ‘[Alexa488/546] GCTGCCACCCGTAGGTGT for Verrucomicrobium, which confirmed the presence of the bacterium. EUB338 ‘[Alexa488/546] 5-GCTGCCTCCCGTAGGAGT-3 [23] was also used. Stool samples with Akkermansia muciniphila were susceptible to imipenem.
Clindamycin was used in the study dealing with development of vancomycin-resistant enterococci (VRE) because this ATB inhibits anaerobes in the intestine without the reduction of facultative Gram-negative bacilli and VRE [24]. It has been shown that clindamycin causes VRE growth in mice and colonised patients [25]. In this study, a mouse model was used to test the hypothesis that the anaerobic microflora in the large intestine inhibits development of vancomycin-resistant enterococci. Anaerobic growth of VRE was assessed in the caecal content and cervical mucus of mice receiving subcutaneous clindamycin and in negative control administered saline solution. Following orogastric inoculation of VRE-Enterococcus Faecium C68, the mice were sacrificed and tested. To confirm that some Gram-positive cocci visualised in this experiment using light microscopy, a specific commercially available kit for the detection of E. faecium by fluorescence in situ hybridization (Microscreen) was used with E. faecium. In saline treated mice, no E. faecium was detected by in situ hybridization. In contrast, the presence of E. faecium was confirmed in clindamycin-treated mice [25].
In a study investigating the role of intestinal bacteria in the pathogenesis of chronic, immuno-mediated inflammation of the intestine, ciprofloxacin has been shown to affect the inflammation of the intestine but not the inflammation of the colon. This has confirmed the selective effect of ciprofloxacin in the gut. Experimental pathogen free (SPF) mice were used. Furthermore, mice lacking the gene encoding interleukin 10 (IL10) producing colitis have been used. However, this does not occur in germ-free mice. Germ-free, IL-10 deficient mice were colonised by SPF bacteria, and narrowed and broad-spectrum ATBs were observed to influence the development and development of intestinal inflammation in IL10 deficient mice. ATBs were administered to mice orally, either preventively prior to colonisation of SPF with bacteria or therapeutically. Quantitative bacterial analysis using the FISH method used parts of the blind and the colon [26]. BAC303 for Bacteroides/Prevotella, E. coli specific EC1531 and other Enterobacteriaceae, Lab158 for the detection of lactobacilli and enterococci were used for FISH detection. By the FISH method, ciprofloxacin was found to reduce total aerobic bacteria in both the colon and caecum. E. coli was not detectable and the number of luminal enterococci was reduced. Reduction of lactobacilli was also confirmed [26].
A study [26] on germ-free, IL10 deficient mice that were colonised by SPF bacteria (no specific pathogens) and were monitored for the effect of a particular narrow spectrum ATB metronidazole on the development of inflammation of the intestine showed a selective effect of metronidazole in the large intestine. The effect of this ATB on inflammation of the cervix was not confirmed. BAC303 for Bacteroides/Prevotella, E. coli specific EC1531 and other Enterobacteriaceae, Lab158 for the detection of lactobacilli and enterococci were used for FISH detection. Metronidazole is selectively effective against anaerobic bacteria, including predominantly Bacteroides. FISH revealed that administration of metronidazole reduced the number of Bacteroides species to a detectable level. Also, the amount of luminal E. coli was significantly reduced. FISH analysis showed that metronidazole had no significant effect on intestinal lactobacilli. Enterococci were confirmed, in particular E. faecalis. The study [23] confirmed that Akkermansia muciniphila were resistant to metronidazole.
ATBs such as tetracycline have the ability to interfere with bacterial populations in the gut. If the formation of a microbial barrier against pathogens and potential pathogens is impaired, it can lead to the proliferation of undesirable microorganisms such as Candida albicans. In in vitro studies, growth of C. albicans was observed in growth media in the presence of tetracycline, with a significant increase in C. albicans. The potency of the probiotic culture of Lactobacillus plantarum LPK, which was added to the in vitro fermentation system, was also tested to determine whether this organism had any effect on the Candida population. Although C. albicans was not completely removed in the presence of this bacterium, its numbers were significantly reduced. This study showed that the use of probiotics, in particular Lactobacillus plantarum, had a positive effect on the reduction of undesirable C. albicans, the number of which was increased by tetracycline administration. It also pointed out that normal intestinal microflora can itself develop a ‘natural’ resistance to C. albicans (Payne et al., [27]). In the future, it would be necessary to use a probe detecting the presence of C. albicans to quantify this bacterium when studying the effect of tetracycline on GIT microbiota. For this purpose, oligonucleotide 020 (5 ‘CCCCCTTTCCTAAACCAATCCGGA 3’) can be used [28].
One of the few studies that dealt with the effect of doxycycline on microbiota using the FISH method was a study aimed at monitoring its effect on Akkermansia muciniphila. For the FISH method, a specific probe 5 ‘[Alexa488/546] GCTGCCACCCGTAGGTGT for Verrucomicrobium was used to confirm the presence of the bacterium. Also, EUB338 ‘[Alexa488/546] 5-GCTGCCTCCCGTAGGAGT-3 [22] was used. In the stool specimen with Akkermansia muciniphila, the sensitivity of this bacterium to doxycycline was confirmed.
In streptomycin-treated conventional mice most of the facultatively aerobic Gram-negative rods, amounting to about 0.1 to 1% of microbiota, were eliminated by streptomycin treatment [29]. Multiple model experiments were used to study the effect of streptomycin on microbiota of mice. To detect the presence and quantify E. coli strains in streptomycin-treated mice, the authors used ribosomal probe ES 1531 specific for E. coli 23S rRNA and E. coli BJ4 reference strain that was detected in stool samples [29]. Also, the adhesion properties of E. coli to colonic mucosa were studied in streptomycin-treated mice and reduced numbers of E. coli were detected [30]. Sekirov 2008 used for the study of the effect of streptomycin on the intestinal microbiota the EUB338 mouse probe for all bacteria (Eubacteriaceae) with the sequence (5 ‘[TxRd]-GCT GCC TCC CGT AGT AGG-3’), Cytophaga-Flavobacterium-Bacteroides CFB286 ‘[Fluorescein]-TCC TCT CAG AAC TAC CCC-3’) and for the Gammaproteobacteria probe GAM42a (5 ‘fluorescein-GCC TTC CCA CAT CGT TT-3’). Sekirov investigated the ability to produce Salmonella infection after ATB treatment [31]. He demonstrated that after the administration of streptomycin, the equilibrium of the microbial community of the intestine changes, giving the possibility of infection with Salmonella. He also found that increasing doses of streptomycin resulted in a gradual increase in the strains of Firmicutes and Cytophaga-Flavobacterium-Bacteroides (CFB). At the genus level, the numbers of lactobacilli and enterococci/group D streptococci decreased significantly. Gradually, the number of Firmicutes and other bacteria was reduced. Sekirov, however, concluded that ATB treatment changes the composition of intestinal microbiota depending on dose and type of ATB, but does not significantly change the total number of gut microbiota [31]. After 8 days of per oral administration, the use of a combination of streptomycin and penicillin caused a significant reduction in all bacterial counts measured by FISH and intestinal content analysis (Swann et al. [32]). Although almost every ATB treatment induces an increase in pathogenic colonisation, the development of enterocolitis was particularly observed after the use of streptomycin or vancomycin (Ferreira et al. [33]). In the current research, three types of mouse models were used to study the interaction between the host and the given bacterium: gnotobiotic, conventional and streptomycin-treated. Studies have shown that mice pre-treated with ATBs (e.g. streptomycin) have a higher chance of competitive growth of intestinal pathogens in the intestine, although the mechanism is poorly elucidated [34]. Streptomycin-treated mice are the best model for studying the growth and survival of extraneous microorganisms in the intestine without causing pathogenesis [35].
Using a FISH method, a study was conducted that investigated the resistance of the Campylobacter strain to macrolide ATB erythromycin. This strain is the most common cause of inflammation of the intestines (enteric). Because its resistance to quinolones rises, macrolides are currently the drug of first choice. In humans, the resistance of Campylobacter to macrolides is about 5%, but in some animals it is up to 80%. Probes for the detection of macrolide resistance in H. pylori were used [36, 37]. The theoretical applicability of these probes for Campylobacter was assessed by controlling previous publications [38, 39]. FISH may also be useful for detecting macrolide resistance in other bacteria, e.g. mycobacteria or haemophiliacs. However, for this purpose, probes must be adapted to different sequences accompanying the mutation point [40].
The effect of clarithromycin as the most commonly used ATB for the treatment and eradication of Helicobacter pylori was studied using the FISH method [36]. In this study, FISH methods were used to demonstrate the presence of H. pylori and to identify the 23S rRNA spot mutation responsible for macrolide resistance directly from a biopsy specimen. All oligonucleotide probes used in this study were previously described and evaluated [41]. Briefly, the HPY-1 (5′-CACACCTGACTGACTATCCCG-3′) probe targeting 16S rRNA was used to identify H. pylori, while the ClaR1 (A2143G) (5′-CGGGGTCTTCCCGTCTT-3′), ClaR2 (5′-CGGGGTCTCTCCGTCTT-3′) and ClaR3 (A2143C) (5′-CGGGGTCTTGCCGTCTT-3′) were used to detect the 23S rRNA spot mutation responsible for the resistance of the bacterium to clarithromycin. A ClaWT probe (5′-CGGGGTCTTTCCGTCTT-3′) was also used to identify H. pylori strains sensitive to clarithromycin that were not detected either by ClaR1, ClaR2 or ClaR3. Similar studies have also been addressed [42].
In mice, the effect of vancomycin on GIT microbiota differs significantly from that of streptomycin [31]. Low doses of vancomycin reduce bacterial counts of Firmicutes and Cytophaga-Flavobacterium-Bacteroidetes (CFB) strains and cause a small increase in the class of Gammaproteobacteria. Higher doses of vancomycin already cause an increase in thecounts of Gammaproteobacteria, to nearly 50% of the total microflora, while the counts of CFB remain reduced. The genera Lactobacillus-Enterococcus, group D streptococci, are affected by the overgrowth of the Enterobacteriaceae and cultivated aerobic bacteria. ATB treatment alone does not cause significant changes in the total number of microbes, although vancomycin administration has a much greater effect on GIT microbiotas than streptomycin [31]. In the study by [43], the broad-spectrum vancomycin-imipenem combination was shown to be effective in mice, both in the cecum and in the colon. Despite the significant decrease in E. coli and E. faecalis, the total aerobic microflora was not reduced after administration of vancomycin with imipenem. However, the amount of total anaerobic bacteria was significantly reduced. Lactobacilli were eliminated after administration of the vancomycin-imipenem combination. Using FISH, it has also been found that by administering this combination, many Bacteroides species have been reduced below a detectable level [43]. Also, vancomycin resistance was investigated in Akkermansia muciniphila [23].
This publication was supported by the Slovak Research and Development Agency under the contract no. APVV-15-0377, project VEGA no. 1/0009/15 and VEGA no.1/0081/17.
This is a brief overview of the main steps involved in publishing with IntechOpen Compacts, Monographs and Edited Books. Once you submit your proposal you will be appointed a Author Service Manager who will be your single point of contact and lead you through all the described steps below.
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\n\nExternal reviewers will evaluate your manuscript and provide you with their feedback. You may be asked to revise your draft, or parts of your draft, provide additional information and make any other necessary changes according to their comments and suggestions.
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I am a Reviewer for several refereed journals and international conferences, such as IEEE Transactions on Biomedical Engineering, IEEE Transactions on Industrial Electronics, Optic Letters, Measurement Science Review, and also a member of the International Advisory Committee for 2012 IEEE Business Engineering and Industrial Applications and 2012 IEEE Symposium on Business, Engineering and Industrial Applications.",institutionString:null,institution:{name:"Joseph Fourier University",country:{name:"France"}}},{id:"55578",title:"Dr.",name:"Antonio",middleName:null,surname:"Jurado-Navas",slug:"antonio-jurado-navas",fullName:"Antonio Jurado-Navas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/55578/images/4574_n.png",biography:"Antonio Jurado-Navas received the M.S. degree (2002) and the Ph.D. degree (2009) in Telecommunication Engineering, both from the University of Málaga (Spain). He first worked as a consultant at Vodafone-Spain. 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