\r\n\tThe development of the interpersonal model and the Kleinian school in the second half of the last century allowed the emergence of an original understanding of the unconscious mind. Within the intersubjective paradigm, the psychoanalytic situation is conceptualized as an interpersonal field to which both the analyst and the patient contribute substantially. We have shown elsewhere how the failure to give a full account of such an intersubjective dimension in both psychoanalytic theory and practice amounts to a core liability in contemporary psychoanalytic discourse.
\r\n
\r\n\tThe present book will focus on a few areas where the insufficient development of our discipline is currently apparent: five wounds that mark the body of the psychoanalytic enterprise.
\r\n
\r\n\tNew contributions are particularly needed in the following areas: Current conceptualization of the unconscious mind is mechanistic and not suited to incorporate the full network of interpersonal exchanges which unfolds in the analytic room; Furthermore, the development of interpersonal psychoanalysis and the theory of the object relations warrants a greater appreciation of the impact of extratranference relations (e.g., couple, family, peers) on the patient's inner life both within and without the psychoanalytic situation.
\r\n
\r\n\tAn integration of theories and models from other psychological paradigms is clearly in order here; the book will also focus on Barangers’ theory of the bi-personal field that makes traditional unipersonal models of the psychoanalytic process untenable. Also, it will help in the understanding of the reciprocal interactions of the two partners in the psychoanalytic dyad in most psychoanalytic institutes the training format relies naively on models from the academic or the professional domains. This fosters rigidity, conformism, and a hierarchical organizational style in the institutional life; e) all over the long span of his creative life Freud showed consistent interest in the application of psychoanalysis to literature, the arts, religion, and politics. Contemporary psychoanalysis is getting more and shyer and is pressed at the margins of social and political debate. The psychoanalytic theory includes unique lore of knowledge about the conscious and unconscious mind. Without it, a comprehensive understanding of human reality will stay out of the reach of contemporary culture.
",isbn:"978-1-80356-882-9",printIsbn:"978-1-80356-881-2",pdfIsbn:"978-1-80356-883-6",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"c6a104ee38fec8d9ba8aa139a33003ce",bookSignature:"Dr. Paolo Azzone",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11591.jpg",keywords:"Unconscious, Repression, Conformism, Intersubjective Paradigm, Interpersonal Psychoanalysis, Object Relation Theory, Couple Therapy, Family Therapy, Psychoanalytic Process, Transference Interpretation, Resistance, Controtransference",numberOfDownloads:3,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 31st 2022",dateEndSecondStepPublish:"June 17th 2022",dateEndThirdStepPublish:"August 16th 2022",dateEndFourthStepPublish:"November 4th 2022",dateEndFifthStepPublish:"January 3rd 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"14 days",secondStepPassed:!0,areRegistrationsClosed:!1,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Paolo Azzone, M.D., is a psychiatrist and a psychoanalyst with over 20 years of experience in mental health topics. 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\n
1. Introduction
\n
Abiotic stress is defined as the negative impact of non-living factors on living organisms in a specific environment. Abiotic stresses, such as drought, salinity, low or high temperatures and other environmental extremes are the major cause of poor plant growth and reduced crop yields in the world [1]. Drought alone affects 45% of the world’s agricultural land, whereas 19.5% of irrigated agricultural lands are considered saline [2, 3]. Moreover, 16% of the agricultural rice land of the world suffers from flash flooding [4]. A combination of two or more abiotic stresses, e.g., drought and heat stress also occurs in field situations and causes more severe crop yield reductions than a single stress [5]. With increasing challenges posed by climate change, it is predicted that warming, drought, floods and storm events will become even more frequent and severe, and will further reduce crop yields, especially in the tropics and subtropics.
\n
Abiotic stresses commonly induce overproduction of reactive oxygen species (ROS) causing extensive cellular damage and inhibition of physiological processes in plants. Although anti-oxidative mechanisms would be an immediate endogenic choice of the plants to counter ROS production, this mechanism can be impaired by abiotic stresses causing a rise in ROS intracellular concentration and an increase in the damage. To survive under such conditions, plants have evolved intricate mechanisms, allowing optimal responses that enable adaptation or avoidance of the stress. These plant responses are regulated at all levels of organization. At the cellular level, responses include adjustments of the membrane system, modifications of cell wall architecture, changes in cell cycle and cell division, and synthesis of specific endogenous and low-molecular-weight molecules, such as salicylic acid, jasmonic acid, ethylene and abscisic acid [6]. An overview of changes that may occur under different abiotic stress conditions is presented in Figure 1.
\n
Figure 1.
Abiotic stress response in plants. Primary stresses, including drought, salinity, cold, heat, and submergence, are often interconnected and cause cellular damage and secondary stresses, such as osmotic and oxidative stresses. The initial stress signals (e.g., osmotic and ionic effects or membrane fluidity changes) are perceived by membrane receptors that transmit the signals downstream to trigger transcription, which is regulated by hormones, transcription factor binding proteins (TFBPs), miRNAs, and transcription factors (TFs) to precisely activate stress responsive mechanisms to re-establish homeostasis and protect and repair damaged proteins and membranes. Inadequate response at one or several steps in the signaling and gene activation levels may ultimately result in irreversible changes of cellular homeostasis and in the destruction of functional and structural proteins and membranes, leading to cell death.
\n
At the genomic level, plant responses include the expression of stress-inducible genes involved in direct plant protection against stresses [3, 7, 8]. A broad range of abiotic stress induced genes are divided into two functional categories: and regulatory proteins. The first group consists of genes encoding for membrane proteins, enzymes for osmolyte biosynthesis, detoxification (glutathione S-transferases, superoxide dismutases, dehydrins, dehydroascorbate reductases, quinine reductases and ascorbate peroxidases) and proteins for macromolecular protection (such as LEA protein, anti-freezing proteins, chaperons and mRNA binding protein) [2]. The second group comprises genes encoding for transcription factors (e.g., DREBPs), protein kinases (e.g., SRK2E), receptor protein kinases, ribosomal-protein kinases and signal transduction proteinases (such as phosphoesterases and phospholipase C). Alterations in the phenylpropanoid pathway in which lignin biosynthesis intermediates are produced also occur under abiotic stress conditions. Moreover, increased accumulation of wall-linked phenolic compounds, for instance, in maize root elongation zone and the polyphenol content in cotton have been linked to stress response [9]. The same authors have shown the role of flavonoids, isoflavonoids, terpenoid and nitrogen-containing secondary metabolites such as glucosinolates alkaloids in abiotic stress response.
\n
Thus, abiotic stress tolerance in plants is a complex trait, involving many different metabolic pathways and cellular and molecular components.
\n
In the past 100 years, conventional breeding (Figure 2; based on observed variation and controlled mating) approaches have randomly exploited these plant tolerance mechanisms with limited success. Moreover, in vitro induced variations have also shown little progress in the improvement of plants against abiotic stresses. These conventional breeding approaches are limited by the complexity of stress tolerance traits coupled with less genetic variation exhibited by most crops due to domestication bottlenecks. The recent reports that the cultivated gene pool of major cereal crops, e.g., rice, maize and wheat, has reduced in genetic variation compared to wild relatives [10–12], raises concern, and could probably undermine the current efforts to identify genetic sources of resistance within the cultivated genepools. It is important, therefore, to consider exploring alternative sources of resistance by incorporating modern techniques into traditional breeding strategies to develop stress-tolerant crops (Figure 2).
\n
Figure 2.
Overview of the traditional and modern approaches in plant breeding. In conjunction with the technological advancements, marker-assisted backcrossing (MABC) and marker-assisted recurrent selection (MARS) schemes, which target an individual marker or set of markers showing significant association with QTLs, are progressively evolving into a modification of MAS, permitting the selection of the desirable genotypes on the basis of genome-wide marker information or genomic selection (GS).
\n
Recently, with the support of genomics, targeted genetic studies involving QTL mapping and validation, identification of key regulatory genes, e.g., genes encoding for ABA receptors, developments in transcriptional and post-transcriptional regulation of stress-responsive genes and studies on hormonal interactions during plant response to stress, have provided opportunities for understanding cellular stress responses in plants. Moreover, the emergence of deep sequencing technologies, proteomics, metabolomics and epigenetics, has remarkably provided novel possibilities to understand the biology of plants and consequently to precisely develop stress-tolerant crop varieties. Amongst the techniques that are currently being exploited to develop stress-tolerant plants, alongside basic molecular biology, there are molecular breeding methods, including development of functional molecular markers to aid in marker-assisted selection, horizontal gene transfer and genome editing tools such as CRISPR/Cas9, to develop genetically modified plants with new or improved characteristics.
\n
In this chapter, we reviewed the plant responses to various abiotic stresses, and focus on genetic and molecular components that function to confer stress tolerance in plants.
\n
\n
\n
2. Advances in plant tolerance to drought
\n
Drought tolerance in plants is the ability to survive and produce stable yields under water scarcity during various stages of crop growth. Principally, drought stress occurs when the soil water potential falls between −0.5 and −1.5 MPa. This affects plants by decreasing the photosynthetic rate through photo-oxidation and enzyme damage, thereby decreasing the amount of assimilates available for export to the sink organs [13]. Besides this, carbohydrate metabolism in plants is severely altered, ultimately affecting both biological and economical yield [14].
\n
Evidence from several studies has shown that plants respond to drought, like many other abiotic stresses, by inducing cellular damage and secondary stresses, such as osmotic and oxidative stresses. These secondary stresses induce initial stress signals (e.g., osmotic and ionic effects and membrane fluidity changes) that are perceived by membrane receptors (sensors). The perceived signals are transmitted downstream to trigger transcription, which is regulated by phytohormones, transcription factor binding proteins (TFBPs), cis-acting elements and miRNAs. Based on the biological functions, the role of these transcriptional regulators and the regulated genes that encode functional proteins or other products to protect plant cells directly from damage is well described [15].
\n
The phytohormone—abscisic acid—acts as a central regulator in the response and adaptation of plants to drought conditions. The various physiological reactions regulated by ABA, including stomatal closure, accumulation of osmoprotectants, changes in gene expression, and other phytohormones have been characterized at the molecular level [16]. The molecular mechanisms of ABA synthesis, transport and signaling in relation to the plant’s response to stress are also reasonably well described [17]. ABA signals are perceived by different cellular receptors. The nucleocytoplasmic receptors PYR/PYL/RCARs (pyrabactin resistance/pyrabactin resistance-like/regulatory component of ABA receptors) have been suggested to be the primary sensors that bind ABA and inhibit type 2C protein phosphatases (PP2Cs) [18]. Inactivation of PP2Cs leads to accumulation of active sucrose non-fermenting-1 (SNF1)-related protein kinases (SnRK2s), which interacts with ABA-responsive TFs, ABA-responsive promoter elements (ABREs) and ABRE-binding protein/ABRE-binding factors (AREB/ABF) to regulate transcription of downstream target genes and related physiological processes [19]. Drought also induces changes in calcium ion levels, which activates calcium-dependent protein kinases (CDPKs) via calmodulin-like domain. The activated CDPKs regulate downstream components of calcium signaling. For instance, OsCPK4 overexpressing rice plants exhibit increased water- holding capacity under drought or salt stress [20]. Genetic manipulation of RLK genes, including OsSIK1 that acts as a positive regulator of drought stress responses, is also well reported [21]. Other secondary signaling molecules, including phosphatases (serine/threonine phosphatases) and phospholipids such as phosphoinositides, nitric oxide, cAMP and sugars, play an important role in signal transduction [22]. Examples of phosphatases include the wheat phosphatase TaPP2Ac-1 that exhibited less wilting under water-deficit conditions than non-transformed controls [23].
\n
Numerous TF families such as myeloblastosis oncogene (MYB), dehydration-responsive element binding proteins (DREB), basic leucine zipper domain (bZIP), WRKYs and the NAC (NAM, ATAF and CUC) are directly or indirectly regulated by endogenous ABA signaling during drought stress [24]. Many MYB genes involved in plant response to drought stress are functionally characterized, including AtMYB15, which was shown to enhance drought tolerance, and sensitivity to ABA [25]. WRKY proteins, including ABA-inducible OsWRKY45, OsWRKY11 and OsWRKY08, are upregulated by drought stress [26]. AP2/ERF family is another large group of plant-specific TFs that have been demonstrated to be effective in enhancing drought tolerance in plants. For instance, overexpression of AP2/ERF genes, e.g., GmERF3 in soybeans, has been reported to enhance tolerance to drought [27]. In addition, DREB2s, e.g., ZmDREB2.7, are candidates for drought stress tolerance in maize [28]. The bZIP TFs have also been reported to enhance plant tolerance to stress and hormone signal transduction, e.g., OsbZIP23 in rice [29] and ZmbZIP72 in maize [30]. Within the NAC family, RD26 (responsive to dehydration 26) was the first NAC gene identified as a regulator in mediating crosstalk between abscisic acid and jasmonic acid (JA) signaling during drought stress responses in Arabidopsis [31]. Overexpression of other NAC genes, including ANAC019, ANAC055 and ANAC072, has been shown to confer drought tolerance in transgenic Arabidopsis [32]. Similarly, overexpression of SNAC1, OsNAC10 and OsNAC5 driven by a root-specific promoter RCc3 confers increased drought resistance under field conditions [33, 34]. The nuclear factor Y (NF-Y) TFs are emerging as important regulators of drought-stress response, particularly with respect to ABA biosynthesis. For instance, ectopic expression of Amaranthus hypochondriacus NF-YC gene (AhNF-YC) in Arabidopsis and overexpression of Bermuda grass Cdt-NF-YC1 in rice has shown that these genes confer drought tolerance [35, 36]. Cdt-NF-YC1 induces expression of both ABA-responsive genes (e.g., OsRAB16A, OsLEA3, OsP5CS1 and OsLIP9) and signaling genes (e.g., OsABI2 and OsNCED3), as well as, ABA independent genes (e.g., OsDREB1A, OsDREB2B and OsDREB1B). In fact there is an increasing evidence that some NAC genes, e.g., SNAC3, contribute to drought resistance and osmotic adjustment independent of ABA [37]. SNAC3 interacts with phosphoglycerate mutase, cytochrome P450 72A1, PP2C, WD domain-containing protein and oxidoreductase to modulate ROS in rice. These findings suggest a complex regulatory mechanisns of drought response and tolerance in plants, involving both ABA and other signaling pathways.
\n
Recent work on inhibitors of phosphoinositide-dependent phospholipases C (PI-PLCs) in Arabidopsis has also provided considerable insight into the drought-stress-related lipid signaling by identifying links of phosphoinositides to the DREB2 pathway [38]. Moreover, overexpression of phosphatidylinositol synthase gene (ZmPIS) in tobacco plants changed membrane lipids’ composition and improved drought stress tolerance [39]. The best characterized lipid derivative, so far, is inositol 1,4,5-trisphosphate (IP3). IP3 levels have been shown to increase in response to exogenous ABA in Vicia faba guard cell protoplasts and in Arabidopsis seedlings, for review see [40]. IP3 acts as a second messenger involved in releasing Ca2+ from internal stores such as vacuoles. This pathway has been reported to induce osmotic-stress-responsive genes, as well as ABA stress-responsive genes [40]. Another lipid derivative, phospholipase D (PLD), has been reported by the same authors to be functionally associated with ABA; and the application of phosphatidic acid (PA), a PLD derivative, has been shown to mimic the effect of ABA in inducing stomatal closure [41]. This could probably suggest that lipid signaling is linked to ABA in drought stress response, and it is worthwhile to study how the different lipid derivatives enter in action, either simultaneously or timely synchronized with ABA.
\n
Downstream of the TFs are numerous responsive genes that function either in a constitutive manner (i.e., also expressed under well-watered conditions) or a drought-responsive manner (i.e., expressed only under pronounced water shortage). Amongst them, genes encoding for receptor-like kinases (RLKs) with Ser/Thr kinase domain could play an important role in optimizing plant responses to drought stress [18]. Other genes that have been shown to be up- or downregulated by drought stress to enable dehydration avoidance or tolerance in various plant species are documented in several studies [18, 42]. Another process, downstream of transcriptional regulatory networks, is the induction of a large range of genes encoding for enzymes involved in osmotic adjustments, osmoprotection, wax biosynthesis and changes in fatty acid composition (Figure 3). Adjustment of osmotic pressure allows the plant to take up more soil water and maintain turgor and cell function for a longer time under drought.
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Figure 3.
Physiological, biochemical, and molecular basis of drought stress tolerance in plants. Both major and minor changes that occur downstream of the transcriptional regulatory network are shown, although some of them, e.g. proline, glycine betaine and other amino acids, were previously shown not to be important in plant resistance to drought stress.
\n
Water-channel proteins, e.g. aquaporins (AQPs), and sugar transporters are believed to facilitate the adjustment of osmotic pressure under stress by transporting water and sugars to the cytosol [42]. More recently, AQPs encoding genes (e.g., MaPIP1;1) were shown to be strongly induced in banana plants exposed to drought [43]. The same authors indicate that overexpression of MaPIP1;1 in Arabidopsis exhibited better growth, reduced water loss and higher survival rates. Li et al. [44] also showed that AQPs were elevated under drought stress in Tibetan Sophora moorcroftiana, which is consistent with the previous reports [45]. However, the same authors indicate conflicting functions of plasma membrane intrinsic proteins (PIPs). For instance, overexpression of GoPIP1, cloned from Galega orientalis, showed increased sensitivity to drought in transgenic Arabidopsis plants. This indicates that AQPs are able to facilitate both tolerance and sensitivity, which warrants further research to delineate AQPs that are potentially helpful in improving drought tolerance in plants.
\n
Studies have also shown that the K\n \n +\n \n\n uptake transporter 6 (KUP6) subfamily of transporters act as key factors in osmotic adjustment by balancing potassium homeostasis in cell growth and drought stress responses [46]. KUP6 is apparently under the control of abscisic acid and interacts with ABA-activated SnRK2-type protein kinase, SnRK2E, resulting in phosphorylation of the KUP6 C-terminal domain. This indicates that KUP6 is a downstream target for SnRK2E in the control of water stress responses. However, other interacting proteins, and probably hormones, e.g. auxins, could regulate the activity of KUP6 in the maintenance of water status during drought stress. Indeed, it was reported previously that a variant of KUP6, KUP4/TRH1, facilitates root-specific auxin distribution [47]. This was substantiated by the findings that triple mutants of the KUP genes (i.e., kup2 kup6 kup8andkup6 kup8 gork) showed enhanced cell expansion and auxin responses in lateral root formation [54]. Moreover, auxin-responsive TFs, LBD18 and LBD29, were highly expressed in the triple mutants in the presence of IAA, indicating that auxin could be modulating K+ and proton fluxes during drought stress.
\n
The biosynthesis of osmoprotectants such as amino acid, amines and carbohydrates is another indispensable strategy for plant resistance to drought stress. The most common osmoprotectants are proline (Pro), γ-aminobutyric acid (GABA), glycine betaine (GB), fructans, starch, mono- and disaccharides, trehalose (Tre) and raffinose family oligosaccharides (RFO). The biosynthesis and transport of trehalose and raffinose is particularly relevant in drought stress response. More recently, genes encoding for trehalose and raffinose biosynthesis were significantly upregulated in the roots and leaves of Jatropha curcas under drought [48], suggesting that these compounds may have major impacts on osmotic adjustment and ROS scavenging during drought stress. The same authors indicated that dozens of genes involved in wax biosynthesis, including KCS and WSD, and their regulators (e.g., MYB96, CER) were upregulated more than four-fold in leaves under drought conditions. Overexpression of genes encoding for MYB96, CER KCS and WSD could probably strengthen the hydrophobic barrier that prevents non-stomatal water loss and increase plant tolerance to drought.
\n
Genes encoding for proteins involved in cellular structure stabilization have also been reported to be induced in plant tolerance to drought. For instance, dehydrins (DHNs) function to protect cells from damage caused by drought stress-induced dehydration [49]. Proteins related to lignin biosynthesis, such as caffeoyl-CoA 3-O-methyl-transferases and class III plant peroxidases, were also found to be induced by drought in wild watermelon [50] and in maize roots [51]. In winter triticale, water-deficit-induced leaf rolling was correlated with a higher level of cell wall-bound phenolics in the leaves [52]. These adaptive mechanism could probably limit water loss by restricting the leaf transpiration surface. In addition, carbon/nitrogen-metabolism-related proteins have been reported to be more abundant in roots of soybean [53], wild watermelon [50] and rapeseed [54] after drought treatment, suggesting an increased energy demand as well as enhanced cellular activities in the root tissues during drought stress. The same authors reported a relative increase in the root growth rate and abundance of root-growth-related small G-protein family members such as Ran GTPases, which suggests increased membrane trafficking activity in an effort by the plant roots to absorb water from deep soil layers.
\n
Taken together, the vast amount of data from ‘omic’ tools provide a basis for identification of more functional genes, which could contribute directly to cellular drought stress tolerance. In addition, understanding expression networks of genes encoding for the aforementioned proteins, especially genes involved in cellular structure stabilization, molecular chaperones, enzymes for detoxification of reactive oxygen species, and those for the biosynthesis of sugars, wax and dehydrins, which are important as protectants [55], may allow for the realization of significant genetic gains in breeding for plant tolerance to drought. Further genomic scale investigations will enable understanding of transcriptional regulators behind co-expressed genes and their association with particular genomic regions (QTLs). Although QTL identification for tracing drought tolerance remains a challenge due to the large number of genes influencing drought tolerance traits, continued investigation into the basis of tolerance in crops like Jatropha curcas will probably provide a clearer understanding of drought tolerance. Besides this, the mechanism by which drought tolerance associated protein networks effectively protect PSII and granal stability, as well as maintain photosynthetic competence will need further elucidation.
\n
\n
\n
3. Advances in plant tolerance to heat stress
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Temperatures above the normal optimum cause heat stress (HS) at different levels in all living organisms. Heat stress disturbs cellular homeostasis, and causes denaturation and dysfunction in many proteins, leading to severe retardation in growth, development and even death. In plants, the major sites of heat stress injury are the oxygen-evolving complex (OEC) along with associated biochemical reactions in photosystem II (PSII). Ultimately, efficiency of electron transport is reduced or altered affecting electron flow from OEC towards the acceptor side of PSII. These alterations affect the generation of ATP and the regeneration of Rubisco for carbon fixation [56]. Starch synthesis is also negatively affected by heat stress because of the reduced activity of enzymes such as invertase, sucrose phosphate synthase and ADP glucose pyrophosphorylase. Usually, ROS induction and accumulation in the chloroplasts precedes these changes. Accumulated ROS can severely damage DNA and cause autocatalytic peroxidation of membrane lipids and pigments, altering membrane functions and cell semi-permeability. Physiological changes associated with biochemical damage may include a decrease in chlorophyll a:b ratio, inhibitions of stomatal conductance and net photosynthesis, and low plant water potential. These changes ultimately reduce the partitioning of photosynthates, which morphologically manifest as retarded growth, reduced economic yield and harvest index. Scorching and sunburns of leaves and twigs, branches and stems, leaf senescence and abscission, and fruit discoloration and damage are other morphological damages associated with heat stress [57].
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Perception of heat stress by plants usually triggers sensors at the plasma membrane and causes a transient opening of Ca2+ channels, possibly via modulation of membrane fluidity (Figure 4) [58]. Upon entry of Ca2+, putatively through channels possessing cytosolic C-terminus with a calmodulin-binding domain, multiple kinases are activated.
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Figure 4.
Hypothetical model for high-temperature signal sensing and induction of molecular pathways leading to plant defence response. Prolonged high-temperature stress causes membrane depolarization leading to Ca2+ influx or directly activates apoplastic enzymes including GLPs. Increased levels of cytosolic calcium activate the ROS-producing enzyme, RBOHD, which catalyses ROS production. Effect of temperature on R genes through an unknown pathway is likely to further enhance ROS production. ROS/ Ca2+ signaling causes activation of plasma membrane ATPase, which extrude H+. Alternatively, heat-stress-induced protein damage and protease activity decreases cytosolic pH. Low cytosolic pH and H2O2 accumulation reduces CO2 assimilation, thereby increasing endogenous carbohydrate metabolism. Cytosolic acidification and ATPase activity may also increase accumulation of expansins and methylesterases that eventually affect the cell wall integrity. Activating plasma membrane ATPase is probably reverse phosphorylated by FKBP65 leading to H+ extrusion and K+ intrusion. A part from its targeted role in the nucleus, FKBP65 could be targeted to the chloroplast through the tat pathway to activate photosystem II 10 kDa polypeptides or for directing chaperone functions. Activated HSPs probably cause chromatin remodelling and histone displacement. In addition to activating PM ion channels, heat-induced changes in membrane fluidity triggers lipid signaling. Plants deploy phospholipids, including phospholipase D (PLD), PIPK (phosphatidylinositol 4,5-bisphosphate kinase), phosphatidic acid (PA), PIP2 (phosphatidylinositol phosphate kinase) and IP3 (D-myo-inositol-1,4,5-trisphosphate) to specific intracellular locations. The accumulation of lipid signaling molecules also triggers Ca2+ influx, which initiates downstream signaling, including activation of CDPKs, hormonal changes, transcription factor activation and secondary metabolism. Question marks indicate the unknown players.
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The MPK6 activity has been particularly shown to increase under heat stress. MPK6 activates a vacuolar processing enzyme (VPE), which has been suggested to play a role in HS-induced programmed cell death [59]. Transcriptional regulators, such as HSFs, WRKY, Zat and MBF1c, a transcriptional regulator of DREB genes [60], are activated to regulate expression of HSPs and other heat stress response genes.
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Heat-induced accumulation of Ca2+ in the cytoplasm also activates the ROS-producing enzymes RBOHD and NADPH oxidase, by direct interaction or through activation of calcium-dependent protein kinases (CDPK) that phosphorylate RBOHD [61]. When activated, RBOHD catalyzes the production of ROS, causing membrane depolarization and/or initiation of ROS/redox signaling network, which interacts with the above-mentioned MBF1c, HSFs, MAPKs and SnRKs to trigger downstream signaling networks [61].
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Calcium/calmodulin-binding protein kinases (CBK), which also regulate the expression of HSPs, are activated via CaM3. A well-known example is the activation of CBK3, which enhances thermotolerance in A. thaliana seedlings by phosphorylating HsfA1a and a CaM protein phosphatase (PP7) [62]. PP7 interacts with both AtCaM3 and AtHsfA1a. AtCaM3 increases thermotolerance by activating WRKY39 and HSFs, indicating that CBK3 plays a key role in heat stress signaling. The TF Zat is necessary for the activation of WRKYs and ascorbate peroxidase [63]. MBF1c modulates the induction of SA and trehalose, which are regulators of plant stress response [64]. SA has been shown to alleviate heat stress by increasing proline production and restricting the formation of ethylene in heat-stressed plants [65].
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Another HS-response-associated signaling pathway was shown in the Hsp90–ROF1 interaction in the cytoplasm and their subsequent translocation to the nucleus. The Hsp90–ROF1 complex localizes in the nucleus only in the presence of HsfA2 [66]. The interaction of these three proteins modulates HSP gene expression under HS. Although, ROF1 has been reported to induce expression of small HSPs, which increases plant survival rate under HS, to date the upstream signal that regulates the subcellular localization of Hsp90–ROF1 remains elusive. Interestingly, just like MBF1c, ROF1 is involved in calcium-dependent phosphorylation of HSFs, which suggests that Ca2+-dependent activation of RBOHD or CDPKs could be the upstream signal for ROF1.
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Heat stress also triggers lipid signaling. Activation of phospholipase D (PLD) and a phosphatidylinositol 4, 5-bisphosphate kinase (PIPK) increases the accumulation of phosphatidic acid (PA), phosphatidylinositol phosphate kinase and D-myo-inositol-1,4,5-trisphosphate (IP3); and an active cycling of a G protein appears necessary in this process. The accumulation of lipid signaling molecules could in turn cause the opening of channels and the triggering of Ca2+ influx [67].
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Downstream effects of heat stress signals have been reported to activate a signaling pathway called unfolded protein response (UPR) in the endoplasmic reticulum, which requires specific calcium signals from the plasma membrane [58]. Within the endoplasmic reticulum, the activity of UPRs involves two signaling pathways: one involving proteolytic processing of membrane-associated bZIP TFs and the other involving RNA splicing factor, inositol requiring enzyme-1 (IRE1) and its mRNA target [68]. IRE1 is a dual functional enzyme possessing both serine/threonine protein kinase and endoribonuclease activity. In Arabidopsis, heat signals activate IRE1 to splice bZIP60 mRNA in the cytosol, causing a frameshift, which triggers the synthesis of a tissue factor without a transmembrane domain, but having a nuclear targeting signal [69]. The bZIP60 (bZIP60(s)) spliced forms activate UPR target genes in the nucleus. A cytosolic form of UPR, which is triggered by the presence of unfolded proteins in the cytosol, was also previously reported [70]. Together, these UPRs are associated with the heat shock promoter elements and the involvement of specific HSFs, notably HSFA2, regulated by alternative splicing and non-sense-mediated decay. Under severe HS (42–45ºC), a novel post-transcriptional regulatory mechanism governing HSFA2 expression has also been shown to occur. Moreover, a new splice variant of HSFA2-III is reported to be generated through the use of acryptic 5′ splice site in the intron. HSFA2-III can be translated into the small HSFA2 (S-HsfA2), which binds to the TATA box proximal clusters of HS elements (HSE) in the HSFA2 promoter to activate its own gene expression, thus constituting a positive auto-regulatory loop [71]. Although the TFs interacting with S-HsfA2 are yet to be validated, this finding suggests that severe HS may alter the splicing pattern of Hsf genes, generating isoforms that may auto-activate self-expression and consequently rapidly induce the expression of HSPs required for enhanced response to HS.
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Apart from HSFs, overexpression of DPB3-1, which regulates expression of DREB2A and DREB2B, increases thermotolerance [72]. Other studies have also shown the role of bZIP28 [73] and WRKY proteins in plants thermotolerance [74, 75]. Furthermore, the basic helix-loop-helix (bHLH) TF, phytochrome interacting factor 4 (PIF4), was reported to control acclimation to changes in ambient temperature by regulating important hormonal and developmental pathways modulating the acclimation mechanisms [76]. PIF4 alleles control floral timing by modulating FLOWERING LOCUS T (FT). PIF4 also controls early inflorescence internode elongation and high-temperature-induced hypocotyl elongation by modulating levels of free indole-3-acetic acid (IAA) through the triggering of YUC8 (YUCCA8) or TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS\n (TAA1) gene expression [57, 77]. Thus, PIF4 is a potential regulator of plant responses to high temperature. However, its physical interaction with cryptochrome 1 (CRY1) on nuclear DNA suggests that these two proteins co-regulate temperature responses in plants. Another regulator, E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), was shown to be essential for plant responses to HS [77]. However, it is not known whether COP1 signaling is independent of PIF4. Orthologs of PIF4 have been identified in several crop species. Thus, if the interaction with other associated proteins is resolved, PIF4 has a potential promise to improve plant tolerance to HS in several crops through genetic engineering.
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Other components of heat sensing that could be linked to these signaling pathways include the transcriptional modulator, the nuclear actin-related protein 6 (ARP6), which is part of the Snf-2-related CREB-binding activator protein (SRCAP) encoding a subunit of the SWR1 chromatin remodelling complex is necessary for inserting the alternative histone, H2A.Z, into nucleosomes, while replacing the core histone H2A [78]. Heat stress induces a decrease in H2A.Z occupancy in nucleosomes located at the transcription start site of heat response genes, a process that probably allows nucleosome opening and enhanced transcription of these genes.
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Plant adaptation to thermotolerance also involves the activity of superoxide reductase (SOR), S-nitrosoglutathione reductase (GSNOR) and rubisco activase (RCA). The functions of these proteins are reasonably well described in a review by [67]. Other commonly reported anti-oxidant enzymes produced by plants under HS include superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST) and non-enzymatic anti-oxidants such as flavanoids, anthocyanin, carotenoids and ascorbic acid (AA) [60]. The accumulation of other osmolytes such as glycine betaine and trehalose is another well-known adaptive mechanism in plants against HS. Generally, most of these compounds are involved in ROS removal (anti-oxidants), osmotic adjustment, saturation of membrane-associated lipids, protection of photosynthetic reactions, production of polyamines and protein biosynthesis [94], which enable plants to exhibit basal or acquired thermotolerance. Proline and glycine betaine application considerably reduce the H2O2 production, improve the accumulation of soluble sugars and protect the developing tissues from HS [79]. Tocopherol is another important lipid-soluble redox buffer and an important scavenger of singlet oxygen species and other ROS. Moreover α-tocopherol has the highest anti-oxidant activity of all the tocopherol types reported in plants [80].
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A number of studies have demonstrated the presence of QTLs associated with most HS-related traits and promise to the use of molecular markers in breeding for heat stress tolerance. More than 50 QTLs have been identified in various crops so far, including maize, wheat, rice, cowpea, lettuce, Medicago truncatula and Brassica napus. Recent studies in transcriptomics [81, 82], proteomics [83, 84], metabolomics [85, 86] and microRNAs [87] have also provided additional information on the mechanisms controlling plant responses to HS. Understanding the relationship between these mechanisms and the genomic regions mapped and delineated as QTLs would validate the genes controlling plant responses to HS, and subsequently improve genetic gains in plant improvement programmes. Besides, the possibility of developing transgenic plants with enhanced tolerance to HS would also gain significance. This approach has already been demonstrated in cotton [88], Arabidopsis [89], tobacco [90] and rice [91], but needs further validation, especially in economically important crops where it has not been applied before. Taken together, heat stress responses discussed here demonstrate that heat stress is a quantitative trait, which requires a combination of several disciplines to improve plant tolerance.
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4. Advances in plant tolerance to cold stress
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Cold stress occurs at temperatures less than 20ºC and varies with the degree of temperature duration and plant type. Chilling (<20ºC) or freezing (<0ºC) temperatures can trigger the formation of ice in plant tissues, which causes cellular dehydration [92]. Ultimately, cold stress reduces plasma membrane (PM) integrity, causing leakage of intracellular solutes. Cold stress severely affects plant growth and survival, and leads to substantial crop losses in temperate climatic regions and hilly areas of the tropics and subtropics [93]. In rice, for instance, losses due to cold stress can range from 0.5 to 2.5 t/ha and grain yields can drop by up to 26%, especially when low temperatures occur during the reproductive stage [94].
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To cope with this adverse condition, plants adapt several strategies such as producing more energy by activation of primary metabolisms, raising the level of anti-oxidants and chaperones, and maintaining osmotic balance by altering cell membrane structure [95]. These mechanisms of plant response to cold stress are closely similar to that of heat stress. However, the difference lies in the fact that membrane rigidification occurs in cold stress as opposed to heat stress. Thus, membrane rigidification is the upstream trigger for the induction cytosolic Ca2+ signatures leading to a transient increase in cytosolic Ca2+levels [96]. It is assumed that dimethyl sulfoxide (DMSO) mediates the perception of membrane rigidification by mechanosensitive Ca2+channels [97]. Other upstream factors such as changes in the metabolic reactions and metabolite concentrations, protein and nucleic acid conformation could contribute to enhance perception of cold stress. These factors as well, either directly or indirectly, induce an increase in cytosolic Ca2+, which is a well-known upstream second messenger, regulating cold regulated (COR) gene expression.
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Cold-stress-induced cytosolic Ca2+ signals can be decoded by different pathways. More recently, Ca2+ signal was reported to be transduced directly into the nucleus. The concentration of nuclear Ca2+ is monitored by a chimera protein formed by the fusion of aequorin to nucleaoplasmin, which is also transiently increased after cold shock [95]. Aequorin possesses several EF-hand-type binding sites for Ca2+ ions. The binding of Ca2+ to these sites causes a conformational change in aequorin which enables the monitoring of Ca2+ concentration. It has been reported that nuclear Ca2+ concentration peaks at about 5–10s later than the cytosolic Ca2+ [95]. The same authors have reported that nuclear Ca2+ signal may be initiated from the nuclear envelope and is assumed to be propagated by cytosolic Ca2+ transients in plants.
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In the cytoplasm, a range of Ca2+ sensors have been reported, including calmodulin (CaM), CaM-like (CMLs), Ca2+-dependent protein kinases (CDPKs), Ca2+-and Ca2+/CaM-dependent protein kinase (CCaMK), CaM-binding transcription activator (CAMTA), calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) [98]. Some of the sensors work as negative regulators of cold tolerance in plants, e.g., calmodulin3, a SOS3-like or a CBL calcium-binding protein and a protein phosphatase 2C (AtPP2CA). The positive regulators, e.g., CDPKs and probably some CBLs, relay the Ca2+ signal by interacting with and regulating the family of CIPKs. For instance, CBL1 has been shown to regulate cold response by interacting with CIPK7 [99], whereas CAMTA3 has been identified as a positive regulator of CBF2/DREB1C through binding to a regulatory element (CG-1, vCGCGb) in its promoter [100]. Although CBF2/DREB1C was previously reported to negatively regulate CBF1/DREB1B and CBF3/DREB1A, its expression appears to be necessary for integrating cold-inducible calcium signaling with gene expression, but under transient and tight control to avoid repression of freezing tolerance. Both CBF1/DREB1B and CBF3/DREB1A are required for constitutive expression of cold-inducible genes in Arabidopsis, and play an important role in cold acclimation (see discussion below).
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Ca2+ influx into the cytoplasm also apparently activates phospholipase C (PLC) and D (PLD), which are precursors for IP3 and PA, respectively. IP3 activates IP3-gated Ca2+ channels that can amplify Ca2+ signatures in the cytoplasm, leading to higher induction of COR genes and CBFs, for review see [101].
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There are some reports that the chloroplast may also play a role in sensing low temperature [98]. Cold stress is considered to cause excess photosystem II (PSII) excitation pressure, as a result of the imbalance between the capacity for harvesting light energy and the capacity to consume this energy on metabolic activity in the leaves, which probably leads to ROS generation. The damaging effect of ROS on the photosynthetic apparatus presumably leads to photo-inhibition, which occurs even under relatively low irradiance [102] and is apparently a mechanism of cold acclimation or freezing tolerance. ROS also acts as the second messenger and may reprogramme transcriptome changes through induction of Ca2+ signatures and activation of MAPKs and redox-responsive TFs. The MAPK cascades in Arabidopsis , including AtMEKK1/ANP1 (MAPKKK)–AtMKK2 (MAPKK)–AtMPK4/6 (MAPK), positively regulate cold acclimation in plants [103].
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The downstream signals that promote the production of COR proteins and cold response to metabolites are reasonably discussed in references [95, 104]. Specific examples include the upregulation of the TFs, CBF/DREB1s (CRT (C-repeat)/DRE binding proteins) [103], which initiate the transcription process. The CBF/DREB1 (mainly CBF3/DREB1A) pathway is controlled by a myelocytomatosis oncogene (MYC)-type TF, inducer of CBF expression1 (ICE1), which binds to the MYC recognition cis-elements (CANNTG) in the promoter of CBF3/DREB1A, and induces the expression of CBF3/DREB1A and its regulons during cold acclimation [105]. The function of ICE1 in cold response is conserved; and overexpression of Arabidopsis\n\n ICE1 improves chilling tolerance and enhances the accumulation of soluble sugars and proline concentration in cucumber [106]. In rice, OsICE1 and OsICE2 are induced by cold stress and sequentially upregulate OsDREB1B, rice heat shock factor A3 (OsHsfA3) and rice trehalose 6-phosphate phosphatase (OsTPP1). The CBF/DREB1s can bind to CRT/DRE cis-elements, A/GCCGAC, in the promoter of COR genes to regulate expression of COR genes [107]. Moreover, CBF/DREB1 genes are organized in tandem (CBF1/DREB1B-CBF3/DREB1A-CBF2/DREB1C) on Arabidopsis chromosome IV and have been reported to be induced at the same time, suggesting that combining these TFs in one genotype could probably improve cold tolerance. However, the inconsistent target specificity amongst the three CBF factors in CBF/DREB1-overexpressing transgenic plants reveals variability in their roles [108]. Indeed, CBF2/DREB1C has been shown to be a negative regulator of both CBF1/DREB1B and CBF3/DREB1A [109], while CBF1/DREB1B and CBF3/DREB1A act as positive regulators of cold acclimation by activating the same subset of CBF/DREB1-target genes [110]. CBF1/DREB1B and CBF3/DREB1A are therefore concertedly required to induce the whole CBF/DREB1-regulon to complete the development of cold acclimation, while the expression of CBF2/DREB1C is tightly controlled to avoid its negative modulation of CBF1/DREB1B and CBF3/DREB1A. The exact mechanism by which this happens is unknown.
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Downstream of these TFs are COR genes, which are mainly linked to the onset of tolerance mechanisms and ultimately lead to acclimation. Genes encoding for annexin; hyper-sensitive-induced response (HIR) protein families (e.g., prohibitins and stomatins); dehydrins (e.g., 25 kDa dehydrin-like protein, ERD14, and cold acclimation-specific protein 15 (CAS15)); anti-oxidants (e.g., superoxide dismutase, catalase and ascorbate peroxidase); HSPs (e.g., HSP70 family being the most abundant); defence-related proteins such as protein disulfide isomerase; disease resistance response proteins, peptidylprolyl isomerase Cyp2 and cysteine proteinase; amino acids, polyamines and polyols; and cellulose synthesis, such as UDP-glucose pyrophosphorylase, are commonly reported in expression studies [111]. Several metabolism-associated proteins, including carbohydrate metabolism enzymes, such as phosphogluconate dehydrogenase, NADP-specific isocitrate dehydrogenase, fructokinase, cytoplasmic malate dehydrogenase, pyruvate orthophosphate dikinase precursors (PPDK), aconitate hydratase, glycine dehydrogenase and enolase, have also been reported to be activated during cold stress [112]. Thus, several genes and the corresponding proteins are associated with the regulation of the metabolic pathways operating under cold stress.
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However, identification of functional polymorphism in these genes remains a daunting task. A similar challenge is observed in the QTLs identified, so far, in various crops, including maize, barley, rice, wheat, sorghum and many other economically important crops. Identification of effective cold sensors also remains elusive, as multiple primary sensors are thought to be involved in sensing low temperatures. Thus, a comprehensive understanding of the defence mechanism from sensors, cold signaling, to the defence response will require further research on both upstream and downstream regulations of ICE1-CBF/DREB1-dependent pathway, as well as proteins that may be functioning independent of this pathway.
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5. Advances in plant tolerance to salinity
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Salinity is increasingly becoming a major threat to crop production, particularly due to inappropriate irrigation regimes and increasing use of brackish water for irrigation. As much as 6% of the total world land is subjected to salinity [113], and more than 20% of irrigated land is affected by salinity [114]. Moreover, major reductions in cultivated land area, crop productivity and quality that have been reported in the recent past are due to salt-induced stress [115]. Climate-change-associated rise in sea levels and coastal floods are expected to further contribute to this phenomenon in the future.
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Salt stress in plants occurs when electrical conductivity of saturated soil paste extract (ECe) reaches 4.0 deci-Siemens per meter (dS/m; approximately 40 mM NaCl). The minimum level may, however, vary from crop to crop. For instance, the salinity threshold for rice is 3.0 dS/m [163]. Beyond this threshold, a yield reduction of 12% per dS/m has been reported to occur. When plants gradually accumulate salts, osmotic stress, nutrient imbalance and oxidative stress occur [116]. These salt effects disrupt intracellular ion homeostasis, membrane function and metabolic activity [117]. As secondary effects, salt-induced osmotic stress decreases root epidermal cell division and elongation rates, reducing primary root growth, eventually resulting in inhibition of growth and reduction of crop yields [118].
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Alkalinity stress is a heightened version of salinity stress which has been reported to be much harsher than equimolar salinity, especially at neutral pH [119]. Although it is fairly understood that alkalinity causes osmotic challenge and ionic stress, and precipitates nutrients such as metallic micronutrients and phosphates, and disrupts the integrity of root cellular structure, the molecular signals and adaptive mechanisms are not well understood. Because many saline soils are also alkaline due to the presence of sodium (Na) carbonates, in this section we will exclusively focus on salinity, which is wide spread, and has been extensively researched and discussed in several studies.
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To cope with saline soils, plants deploy a range of mechanisms that range from exclusion of Na+ from the cells to tolerance within the cells. When plants are subjected to salinity, a series of responses ranging from genetic molecular expression through biochemical metabolism to physiological processes occur (Figure 5).
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Figure 5.
Adaptive mechanisms of salt tolerance. Cellular functions that would apply to all cells within the plant are the first adaptation mechanisms, followed by the functions of specific tissues or organs. Most of these functions are explained in the text (modified from [140].
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Amongst the receptor proteins identified as the first detectors of salt stress are G-protein-coupled receptors, ion channel, receptor-like kinase or histidine kinase. These receptors transduce signals that generate secondary signals such as Ca2+, inositol phosphates, ROS, nitric oxide (NO) and ABA. The signaling pathway associated with increased concentration of cytosolic Ca2+ is the most reported.
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Cytosolic Ca2+ activates calcium-dependent protein kinases (CDPKs), calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) to transduce signals to downstream protein activity and gene transcription [120]. Transcription factors such as calmodulin-binding transcription activators (CAMTAs), GT element-binding-like proteins (GTLs) and MYBs have been reported to be activated by Ca2+/calmodulin directly [121–123]. Other commonly expressed TFs in response to salt stress include the basic leucine zipper (bZIP), e.g., OsbZIP71 in rice [124], WRKY [125], APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) [126], MYB [127], basic helix–loop–helix [128] and NAC [42] families. These TFs regulate the expression of genes related to water potential decrease, which results from osmotic stress caused by salinity.
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Downstream of these TFs, there are several genes associated with salinity tolerance. The most reported are genes encoding for salt exclusion proteins, e.g., SOS1, cation:proton antiporter family1 of Na+/H+ anti-porters, salt compartmentalization genes, e.g., vacuolar H\n +\n -pyrophosphatase [129], and osmotic adjustment, e.g., pyrroline-5-carboxylate synthetase [130].
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The salt overly sensitive (SOS) Ca2+ sensor regulatory mechanism is believed to be conserved in higher plants including monocots and dicots [131]. SOS consists of three functionally interlinked proteins, SOS3/SCaBP8–SOS2–SOS1. SOS3 mainly functions in the roots, while CBL10/SCaBP8, an alternative regulator of SOS2 that has been described as SOS3-like, primarily functions in the shoots. At high Na+ concentrations, increased influx of Ca2+ is perceived by SOS3 that encodes a myristoylated EF hand (a domain of five serially repeated helix–loop–helix calcium-binding motifs). Upon Ca2+ binding, a conformational change occurs and SOS3 activates the downstream serine/threonine protein kinase, SOS2, and recruits it to the plasma membrane. Subsequently, the SOS3–SOS2 complex stimulates the plasma membrane-localized Na+/H+ anti-porter (SOS1), leading to the extrusion of the excess Na+ out of the cells [132]. Different from SOS3, SOS3-like proteins (CBL10/SCaBP8) are phosphorylated by their interacting protein kinases apparently regulating CBL/SCaBP–CIPK/PKS modules [133].
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Besides extruding Na+, the adaptive SOS module also links cytosolic Na+ with Ca2+ binding proteins. The PM-localized NHX7/SOS1 and the intracellular localized cation:proton antiporter family1 (CPA1) of Na+/H+ anti-porters (NHX1-NHX4; tonoplast-localized) are a ubiquitous family of transporters that mediate the exchange of K+ or Na+ for H+ while regulating cytoplasmic salt overloads [134]. In the cytosol, increased influx of Ca2+ associated with excess Na+ levels is perceived by Ca2+-binding calmodulins/calmodulin-like proteins, which interact with NHX1 transporters to sequester excess Na+ in the vacuole. In Arabidopsis, a calmodulin-like protein, AtCaM15, regulates the tonoplast localized AtNHX1 [135]. The interaction of AtCaM15 with AtNHX1 occurs in the vacuolar lumen and is dependent on Ca2+ and pH. The C-terminus of AtNHX1 has been shown to localize in the cytosol, which might suggest that this strategic placement is targeted for phosphorylation by protein kinases or for sensing changes in cytosolic pH. However, the protein kinase targeting AtNHX1 is unknown, and further studies on the interaction of this transporter with other proteins, especially protein kinases, will be necessary.
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Interestingly, at moderate salt levels, the role of these transporters is less clear. Indeed, the nhx1/nhx2\n\n double mutants are not sensitive to moderate external Na+ concentrations, yet they are sensitive to moderate external K+ concentrations, for review see [134]. Conversely, the trans-Golgi network-localized NHX double knockouts, nhx5/nhx6, highly respond to moderate salinity and interfere with vesicle trafficking to the vacuole. This suggests that the endosomal NHXs are more sensitive to Na+ accumulation than vacuolar NHXs. This difference has implications on Na+ tolerance in plants. Recently, another CPA family member, a cation/H+ exchanger (CHX), GmSALT3, was shown to improve shoot Na+ exclusion and salt tolerance in soybean [136]. Fluorescent protein fusions suggested that GmSALT3 and other CHX proteins are localized to the endoplasmic reticulum, further indicating that endosomal NHXs could be more reliable in sensing abnormal Na+ levels in the cell and has a positive implication on salt tolerance in plants.
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Other genes encoding for Mannose-1-phosphate guanyl transferase\n\n (OsMPG1) and the rice homologue of Shaker family K+ channel KAT1 (OsKAT1) have also been reported to confer salinity tolerance [137, 138]. OsMPG1 is an important enzyme for the biosynthesis of ascorbic acid in plants, whereas OsKAT1 reduces the cellular Na+ to K+ ratio by increasing the cellular K+ content. Another rice potassium transporter (OsHAK5) was shown to accumulate more K+ and less Na+ when constitutively expressed in Nicotiana tabacum cv. Bright Yellow 2 under salinity stress [198]. Several other genes were recently identified by Chen et al. [139] while studying the halophyte seashore Paspalum (Paspalum vaginatum).
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Another process, downstream of transcriptional regulatory networks, involves accumulation of sufficient solutes (e.g., proline and glycine betaine) to balance extra osmotic pressure in the soil solution to maintain turgor [140]. Moreover, plants can also accumulate sufficient Na+ and Cl− to balance those in the soil solution, but this is tightly controlled through strict ionic regulation in various cell compartments (‘tissue tolerance’). These tolerance strategies are achieved through a series of ion transporters and their localization in key cell types. Na+/H+ anti-porter proteins are the key regulators of these tolerance strategies. Examples include TaHKT1;5-D protein, which maintains high cytosolic K+/Na+ ratios in bread wheat shoots by restricting Na+ loads in the root xylem before entering the shoot [141]. Recently, the introgression of the Triticum monococcum HKT1;5-A into durum wheat improved shoot Na+ exclusion and improved grain yield in the field by 25% [142], indicating the significance and functional stability of these transporters even in interspecific hybrids. Additionally, Eswaran et al. [143] used the yeast Full-length cDNA Over-eXpressor (FOX) gene hunting to identify several salt-responsive genes in Jatropha curcas. The late embryogenesis-abundant protein (LEA-5), aquaporins and a cytosolic ascorbate peroxidase-1 (Apx1) were amongst the identified genes involved in salinity tolerance. LEA5 are group 5 LEA genes that have been shown to play roles in the combining of concentrated ions and dehydration [143]. This group of LEA proteins have attracted fewer investigations and will require further studies at salt stress conditions. Aquaporin proteins are members of a large multigenic family that regulates a large proportion of water transport across membranes. Aquaporins are rapidly influenced both transcriptionally and post-translationally, and enhance salt stress tolerance in plants. For instance, a plasma membrane intrinsic protein (GmPIP1;6, which belongs to a subfamily of aquaporin specifically located in the PM) in soybean increases shoot Na+ exclusion and improves the seed yield from a saline field [144]. Orthologous PIP proteins are found in Arabidopsis , tobacco, barley, rice and wheat. For instance, GmPIP1;6 is the ortholog of AtPIP1;2, NtAQP1, HvPIP1;6/1;1 and TaAQP8. Overexpression of NtAQP1 in tobacco increases photosynthetic rate, water use efficiency and yield under salt stress [145]. Overexpression of TaAQP8, TaNIP and TaAQP7 genes in Arabidopsis or tobacco also increases salt tolerance of transgenic plants [146–148]. Root stellar cells also confer control over shoot Cl− accumulation [149]. The expression of GmPIP1;6 in roots was recently shown to be correlated with rapid and longer term changes in root hydraulic conductance (L\n\n o) in response to shoot treatments and appeared to be more concentrated in stellar tissue [150]. These results indicated that GmPIP1;6 could be the protein responsible for the control of root water transport, particularly in response to shoot signals. More recently, overexpression of GmPIP1;6 was shown to significantly increase salt tolerance of soybean by improving root L\n o and Na+ exclusion, which provided additional evidence that GmPIP1;6’s activity is in the stellar tissue. However, as there is no conclusive interactive or independent role of AQPs in salt tolerance, AQPs could instead be playing an indirect role through their impact on osmotically driven water and solute flow in roots and leaves. Further research will probably provide clear insight as to whether GmPIP1;6 is responsible for salt regulation in the stellar cells, and whether there are other co-factors involved.
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Wheat tonoplast intrinsic protein (TIP2; 2) is also reported to enhance salt tolerance [151]. However, the functional role of this protein is regulated by methylation following salt treatment as is HKT1 in Arabidopsis [152]. This suggests that aquaporin methylation could also play a role in regulating salt tolerance in plants and is worth further exploration.
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Accumulation of ROS scavenging enzymes has also been reported to lower cellular damage, maintain photosynthetic energy capture, and improve shoot and root growth under saline conditions. For instance, salt-stress-induced accumulation of SOD has been reported to play a protective role in Canola, S. europaea, S. chilense and K. candel [153–155]. Furthermore, expression levels of anti-oxidant enzymes APX (e.g.,\n Apx1)\n\n , Trx, Prx, GPX and GST were observed to be enhanced in Tangut nitraria [156] under salinity conditions. Moreover, the same authors have reported that a photosynthetic enzyme, Ferredoxin—NADP (+) reductase (FNR), activity also increased in T. nitraria. Pea plants grown under saline stress also showed an enhancement of both APX activity and S-nitrosylated APX, which suggests that APX plays a significant role in plant tolerance to salt stress. However, apart from ascorbic acid biosynthesis, which has been shown to be modulated by OsMPG1, the molecular regulation of most anti-oxidants in response to salinity remains to be explored.
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The recent discovery that salt-tolerant plant growth promoting rhizobacteria (PGPR) populations reduce Na+ concentration in the plant shoots [157] provides further insights into plant tolerance to saline conditions. The PGPRs increase the expression of stress-responsive TFs, induce greater proline synthesis, enhance ROS scavenging and improve plant biomass under salinity stress. Therefore, treatment with rhizospheric organisms, and understanding the mechanisms associated with these PGPRs leading to salt tolerance, is an attractive option to improve crop yields under saline conditions.
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Fundamental insights into genetic control of salt tolerance mechanisms have also led to identification of more than 100 QTLs in various crops including Arabidopsis , barley, rice and wheat, amongst others. The earlier mentioned salt overly sensitive (SOS) pathway genes and AtCIPK16 are amongst the salt tolerance factors spanning several QTLs identified [158]. CIPK16 is an SNF1-related kinase/CBL-interacting protein kinase, underlying a quantitative trait locus for Na+ exclusion in the Arabidopsis Bay-0×Shahadara mapping population. CIPK16 was also recently shown to be expressed in barley and improves Na+ exclusion and biomass in a saline field.
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Taken together, several genes and proteins have been shown to enhance salt tolerance in plants. However, the limited number of genes with functional polymorphism for salt tolerance makes it difficult to employ marker-assisted breeding for salt tolerance traits. In addition, the complex molecular mechanisms underlying the difference between seedling and reproductive stage salt tolerance in plants, e.g. rice [159], suggest the need for further research. The importance of the apoplastic bypass flow in delivering Na+ to the xylem, thus reducing leaf Na+ concentration and improving tolerance as suggested by [160], is also worth exploring further. Moreover, more insights into the molecular regulation of salt response will provide avenues for combining tolerance mechanisms to develop varieties that are widely adapted to salt stress.
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6. Advances in plant tolerance to submergence/flooding
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Over the past 25 years, yield losses caused by flooding have been increasing in various parts of the world, including the United States, China, Europe, Pakistan and Australia [161, 162]. Flooding is expected to increase as a result of erratic weather patterns, including frequent and lengthy storms associated with climate change, and could severely affect food production if mitigation measures are not sought.
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Generally, submergence/flooding stress results from reduced oxygen levels in the plant root zone due to the low diffusion rate of oxygen in water. Submergence inhibits electron flows that underpin photosynthesis and aerobic respiration from the air causing energy shortfalls that can prove injurious to the plant [162]. Flooding also leads to accumulation of gases such as ethylene and carbondioxide by preventing their diffusive escape and oxidative breakdown [163]. A high concentration of ethylene limits root extension, while carbon dioxide can severely damage plant roots. Trapped carbondioxide may also form bicarbonate ions that can accentuate the effect of high lime content, leading to iron unavailability and chlorosis. The hypoxic environment also leads to restricted production of ATP, forcing cells to rely on glycolysis and fermentation to generate ATP and regenerate NAD+ to cope with the energy crisis [164]. Moreover, survival through prolonged inundation hypoxia involves the use of inorganic pyrophosphate (PPi) as an alternative energy source and induction of enzymes that reduce reactive oxygen species (ROS) or cytoplasmic acidosis, which are equally energy consuming processes. Because translation is a tremendously energy-intensive process, protein synthesis is affected in such oxygen-deprived conditions. Subsequently, essential metabolic processes slow down affecting the overall growth of the plant. In rice, soybean and wheat, various deleterious effects have been observed, such as suppression or reduction of hypocotyl and root elongation, and suppression of lateral root development [162, 164, 165].
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Plant tolerance to submergence/flooding is generally a metabolic adaptation in response to anaerobiosis that enables cells to maintain their integrity so that the plant survives hypoxia without major damages. Several defence-related changes occur in submergence tolerant plants, including anatomical (e.g. formation of higher aerenchyma tissue in the nodal region in rice), physiological (more shoot elongation) and biochemical (inhibition of chlorophyll degradation, less utilization of storage carbohydrates and increased activity of anti-oxidative enzymes). At the molecular level, plants need to adapt these several changes in their gene expression profiles as well as cellular protein profiles. We will focus more on molecular adaptation, with a preference for adaptive QTLs, genes and proteins of significance to crop tolerance to flooding.
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One of the early responses to submergence involves the differential regulation of a suite of TFs belonging to the ethylene response factor (ERFs) gene family. In rice, a major QTL locus belonging to ERF family, which is responsible for submergence tolerance, was mapped to chromosome 9, designated as Submergence1 (Sub1) [166]. This QTL was reported to account for about 70% of the phenotypic variation under submergence [167]. One of the genes adhered to Sub1 locus is Sub1A, which limits shoot elongation during submergence by repressing gibberellic acid (GA) levels and modulating GA signaling. In the process, the consumption of energy reserves is reduced, and upon de-submergence, genotypes with SUB1A are able to resume development when flood water subsides.
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Two ERFs, SNORKEL1 (SK1) and SNORKEL2 (SK2) from Thai deep water accession C9285, also confer submergence adaptation in deep water rice by inducing rapid internode elongation [168]. SKs have also been found in the genomes of accessions of wild O. rufipogon from Asia and O. glumaepatula from South America but missing in most cultivated rice varieties, which suggests that an ancient genomic region of Oryza was lost during the establishment of rice grown in shallow paddies, but was safeguarded in deep water ecosystems. More recently, two QTLs on chromosome 3 and 12, including O. sativa-GROWTH-REGULATING FACTOR7 (OsGRF7), were reported to be involved in GA-dependent stem elongation and meristem maintenance in deep water rice [169]. OsGRF7 on chromosome 12 could probably be a regulator of GA responsiveness for internode elongation, whereas a QTL on chromosome 3 and other QTLs may regulate the DELLA function or act downstream of GA signaling. The DELLA proteins are the key regulators of GA signaling and suppress plant growth in the absence of GA.
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In maize, a major QTL, Subtol6, was also recently shown to be associated with submergence tolerance [170]. Based on the expression differences between the parent inbreds, subtol6 is associated with HEMOGLOBIN2 (HB2), a gene which was previously reported to be associated with plant survival in low oxygen or low ATP conditions [171]. The same authors indicate that haemoglobin proteins in maize repress ROS levels and maintain the energy status of maize cells during hypoxia. Other notable candidate genes, including genes related to ABA-INSENSITIVE3 (ABI3)/VIVIPAROUS1 (RAV1), genes related to accumulation and metabolism of carbohydrates, e.g., alpha subunit of PYROPHOSPHATE-DEPENDENT FRUCTOSE-6-PHOSPHATE 1-PHOSPHOTRANSFERASE (PFP) and ALCOHOL DEHYDROGENASE1 (ADH1), have been reported to be highly upregulated in response to submergence [170].
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In association with these tolerance genes, a number of other QTLs have also been identified in various crops, including barley, wheat, Brassica napus, maize and Lolium perenne, amongst others.
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In addition to these QTLs studies, several proteins have been reported to enhance submergence tolerance in plants. Enzymes involved in primary metabolism are differentially regulated in response to flooding. For instance, UDP-glucose dehydrogenase, UDP-glucose pyrophosphorylase, β-glucosidase G4 and rhamnose synthase, aspartate aminotransferase and lipoxygenase have been reported as early flood-responsive proteins in rice and soybeans [164, 172]. The same authors indicate that phenlypropanoid pathway and cell wall synthesis enzymes decrease in abundance during flooding, which could be an energy-conserving adaptive strategy towards enhanced flooding tolerance.
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Together these findings suggest that during flooding several processes are inhibited to reduce energy consumption. It is crucial for the plant to preserve some carbohydrates for release of energy to support further growth when the water level recedes. The regulatory genes in this category may also serve some ABA-mediated water stress recovery and inhibition of GA-induced internodal elongation as quiescence strategies adopted by plants [173]. On the other hand, avoidance mechanisms employed under deep water conditions involve rapid internode elongation. In R. palustris, there are populations that show either the quiescence response or the avoidance response to submergence. This divergence shows that quiescence and avoidance are two strategies that can be employed by plants depending on the duration of flooding. Quiescence can be the optimal strategy for short-duration ‘flash’ floods, whereas avoidance via growth could be more reliable in prolonged deep flooding. Notwithstanding the above-mentioned tolerance genes and proteins, a deeper insight into the molecular regulation of quiescence and avoidance, and the associated regulatory networks, is still needed to provide sustainable avenues for improving plants specific to either flooding condition or able to grow in both.
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7. Advances in plant tolerance to nutrient imbalances
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7.1. Tolerance to nutrient deficiency
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A total of 21 mineral nutrients are essential for crop growth and development. Most nutrients in the soil are primarily generated from the weathering of the parent material in the Earth\'s crust. Moreover, nutrient levels can vary widely across locations because of initial influence of the composition of the parent material. In most cases, inadequate replenishment from the parent material and from the adsorbed and complexed fractions causes nutrient deficiencies in the soil. In addition, natural factors, including acidity, alkalinity and human activities such as inadequate fertilization also cause nutrient deficiencies. In countries such as India and China, mineral deficiencies have significantly stagnated or limited crop yields. More than 30% of agricultural soils are boron deficient, not only in China and India, but in the whole world. Moreover, zinc deficiency is even more widespread, affecting approximately 50% of the soils. Significant zinc deficiencies occur in sub-Saharan Africa, Turkey, Iran and Pakistan [174].
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Several studies have been conducted on understanding plant nutrition; the most noteworthy being the work of the German scientist Justus von Liebig, who stipulated that plant growth is controlled not only by the total resources (nutrients) available, but also by the scarcest resource (the limiting factor). This submission has stimulated a series of studies on nutrient management, including plant breeding for tolerance to nutrient deficiencies. Tolerance to nutrient deficiency is associated with the genotype’s nutrient use efficiency. Genotypic variation in nutrient use efficiency is closely related to root nutrient acquisition capacity and utilization. In this section, we will focus on nitrogen and phosphorus, the two most limiting nutrients that are essential for several biological processes in plants.
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7.1.1. Plant tolerance to nitrogen deficiency
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Nitrogen is the most limiting nutrient to plant growth in most ecosystems despite its abundance in the atmosphere. This problem occurs because most plants can only take up nitrogen in two solid forms: ammonium ion (NH4\n +) and nitrate ion (NO3\n\n −). Ammonium is used less by plants because it is extremely toxic if taken up in large concentrations, so inorganic nitrate is the most usable form obtained by plants from the soil solution. Nitrogen-deficiency effect on crop yields depends on the growth stage at which it occurs, as well as on its duration and extent [175]. However, reduced radiation interception, low radiation use efficiency, poor dry matter partitioning to reproductive organs, reduced leaf area index and decreased protein content of the plant and seed are the common effects of nitrogen deficiency.
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Plants react in many different ways to changes in N provision; and physiological and molecular components governing N uptake, assimilation and remobilization during the plant life cycle have been studied extensively in the past decades, for review see [176, 177]. Three types of responses have been recently unraveled: (i) regulation of root N uptake systems, (ii) plasticity of root system architecture and (iii) fast modulation of shoot growth [178]. The first two responses generally improve efficiency of root N uptake under deficient conditions. The upregulation of specific high-affinity membrane transporters and enhanced foraging by the root system are implicated in these responses. When soil conditions for N uptake are seemingly unfavourable, e.g. limited water availability, plants will quickly slow down the overall N demand, as a nutrient conserving adaptive strategy, to prevent N starvation until conditions for N uptake become favourable.
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In various plant species, nitrate transporters play a dominant role in N uptake. In Arabidopsis, three major families of nitrate transporters have been identified: Chlorate resistant 1 (CHL1/NRT1), NRT2 and chloride channel (CLC) [177]. NRT2 belongs to the high-affinity nitrate transporter group while most of the NRT1 family members belong to low-affinity nitrate transporters. The only exception, so far, in the latter group is NRT1.1 that is a dual affinity nitrate transporter. Thus, the high-affinity transporters that have been identified and primarily associated with nitrate uptake from the external environment include NRT1.2, NRT2.1, NRT2.2 and the dual affinity transporter, NRT1.1.
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\n\n NRT1.1 is functionally regulated by phosphorylation of a threonine residue, Thr101, which facilitates the switching of its activity from a low- to a high-affinity state. AtNRT1.1, which is also induced by auxin and is itself an auxin influx facilitator, is a dimer in the asymmetric unit cell despite being monomeric in solution. At low nitrate levels, AtNRT1.1 is phosphorylated at the dimer interface, dissociates the NRT1.1 dimer, changes into a high-affinity transporter and represses lateral root (LR) development by promoting basipetal auxin transport out of LR primordia (LRP) [179]. At high nitrate levels, NRT1 1 is dephosphorylated, adopts a dimeric structure and adapts a low-affinity transporter configuration. In this state, trafficking of auxin out of the LR is blocked, and auxin accumulates in the LR initials promoting LR development. NRT1.1 is also shown to act upstream of the MADS box ARABIDOPSIS NITRATE REGULATED1 (ANR1) when modulating LR growth [179]. ANR1 mediates localized N response and modulates the proliferation of LRs in N-dense patches. Moreover, NRT1.1 has been shown to regulate genes encoding for other nitrate transporters, including NRT2.1 and NRT3.1 [180]. However, NRT1.1 and NRT2.1 are localized in different cell layers in the roots, and their adaptive/complementary strategy in nitrate uptake is not well elucidated. The NRT1.1-auxin modulation and nitrate signaling has also been a topic of interest and requires elucidation [181].
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Amongst the CLC family members, CLCa and CLCb function as proton-nitrate exchanges, and have high selectivity for nitrates over chlorides [182]. Both transporters are known to mediate nitrate accumulation in the plant vacuoles. Besides the above-mentioned transporters, the acquisition of nitrate is also regulated by slow anion channel (SLAC1) and SLAC1 homologue (SLAH) and nitrate excretion transporter (NAXT-1). Five SLAC genes were previously reported in Arabidopsis . Amongst these genes, SLAC1 and SLAH3 show nitrate transport activity, but their channel activity is co-regulated by kinases (e.g., CPK21) [183]. An efflux component operated by NAXT-1, associated with the nitrate transporter 1/peptide transporter (NRT1/PTR) family of proteins, mediates nitrate efflux under acid load in the cytosol [184]. Similarly, NRT1.5, which loads nitrates into the xylem for root-to-shoot translocation, also mediates nitrate efflux. However, the proton-coupling mechanism of NAXT1 remains to be elucidated. Two other transporters, NRT1.8 and NRT1.9, have been reported to regulate root-to-shoot nitrate translocation [185, 186]. Both transporters are apparently negative regulators of root-to-shoot nitrate transport. The subsequent nitrate allocation into the vegetative tissues, reproductive tissues and osmotic regulation of guard cells is reasonably described elsewhere [187].
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Further studies on signaling, transcriptional and post-translational regulation have revealed evidence that a CBL-interacting protein kinase, CIPK8, regulates the activity of nitrate transporters and the expression of nitrate assimilation genes [188]. Like CIPK8, CIPK23 is also suggested to be activated by a CBL protein, CBL9, but the exact mechanism is elusive. CIPK23 directly interacts with NRT1.1 in the plasma membrane and phosphorylates NRT1.1 at Thr101 to adopt a monomeric structure when the nitrate concentration is low. This process helps plants to adapt to low nitrogen levels.
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Several TFs have been implicated in regulating NRT1.1 activity. For instance, the activity of two bZIP TFs in Arabidopsis, ELONGATED HYPOCOTYL5 (HY5) and HY5-HOMOLOG (HYH), was suggested to positively modulate NITRATE REDUCTASE2 (NIA2) and negatively modulate NRT1.1 [189]. The NODULE INCEPTION (NIN)-like TFs have also been shown to play a central role in the regulation of nitrate-inducible genes [190]. Nitrate signaling activates NIN-like transcription factors through their N-terminal regions. The activated factors promote the expression of nitrogen assimilation-related genes and genes encoding regulatory proteins. NLP7 is the most reported in this family of TFs. NLP7 is strongly induced in vascular tissues and root hairs, and is required for the induction of several nitrate uptake and assimilatory genes. Thus, NLP7 is is probably a key regulator of nitrogen utilization mechanisms. More recently, the presence of nitrate in the external solution induced the expression of NRT accessory proteins (NAR), nitrate reductase, nitrite reductase and genes involved in the GS-GOGAT cycle, in Arabidopsis, as well as in maize and other plants [191]. These proteins likely play a role in nitrate sensing.
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Strigolactones (SLs), a new class of plant hormones and rhizosphere signaling molecules, also appear to be upregulated in plants under low N conditions [192]; however, the impact of SL levels on root growth is yet to be determined. Changes in root system architecture (RSA) may also be induced depending on the prevailing available organic form of nitrogen, for review see [118]. The most commonly reported organic forms are l-glutamate or carnitine. In Arabidopsis seedlings, l-glutamate inhibits cell division in the root apical meristem (PRM) of the primary root (PR) tip and promotes LR formation and outgrowth. However, several Arabidopsis auxin-signaling mutants display different levels of sensitivity to l-glutamate, suggesting that l-glutamate is rather a signaling molecule as opposed to a nitrogen source [193]. In addition, the rice glutamate receptor mutants display a host of RSA changes, including short PR and LR, reduced cell division and the cell death of root apical meristem [194], further suggesting that l-glutamate is a signaling molecule. l-Glutamate could be a major anchor in the signaling process leading to nitrate uptake and assimilation. This is supported by previous studies that have shown that glutamine synthetase (GS1) from alfalfa causes an increase in photosynthesis and growth under low N fertilization regime [195]. Glutamine synthetase also mediates ammonium assimilation into glutamine. Ammonium form of nitrogen is rapidly assimilated into organic nitrogen forms to avoid tissue toxicity, for review see [196]. Several other reviews have documented the genes and proteins regulating nitrogen use efficiency (NUE) in plants. The reader is referred to excellent reviews by [177, 196]. In addition, more than 50 QTLs for nitrogen use efficiency have been reported in plants, though few of them have been validated. Amongst the identified QTLs are nitrogen deficiency response QTLs in rice, nitrogen supply responses and yield in wheat and nitrogen use efficiency in barley.
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Collectively, nitrogen use efficiency in plants is controlled by a complex array of physiological, developmental and environmental interactions that are specific to the genotype of a given species. Notwithstanding the aforementioned N uptake and utilization genes and QTLs, an extensive molecular survey of a wide range of genotypes covering the genetic diversity of a crop could provide further evidence on the genetic control of these trait. This can be achieved using the various available ‘omics’ techniques, combined with agronomic and physiological approaches in order to identify more elements controlling NUE in plants, both universal and specific, for use in crop improvement.
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7.1.2. Plant tolerance to phosphorus deficiency
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Phosphorus (P) is the second most limiting mineral nutrient in almost all soils, and >30% of the world’s arable land has low P [197]. Phosphorus availability is particularly limiting on highly weathered acid soils of the tropics and subtropics due to its fixation by Al and Fe oxides on the surface of clay minerals. Plants take up phosphorus as phosphate (Pi), either directly by the root system or transferred through the fungal symbiont in arbuscular mycorrhizae host plants. Plants have elaborate sensing and signaling mechanisms in response to Pi deficiency, and both local and systemic signaling in response to Pi deficiency have been reported [197]. Key responses in the plant include changes in the root system architecture (RSA), a reduction in photosynthetic rate; increased activity of high-affinity Pi transporter activities; secretion of APases, ribonucleases and organic acids; membrane phospholipid replacement with glycolipids and sulfolipids; and increased availability of anthocyanin and starch [198]. Putative signaling molecules in response to Pi deficiency include sugars, hormones and microRNAs.
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Under limiting Pi conditions, plants can monitor Pi deficiency both locally and systemically, and root foraging strategy to explore top soil layers for Pi is employed. The Pi foraging strategy is accomplished through one of the several different RSA and physiological changes [118]. The local external Pi rather than the systemic Pi status of the whole plant regulates the remodelling of RSA [199]. In maize and some species in the Proteaceae and Casuarinaceae families, the remodelling of RSA involves production of adventitious roots and cluster roots [200, 201], which increases root surface area for Pi absorption. While a plant Pi receptor is yet to be identified, recent reports have suggested that ethylene biosynthesis and signaling are involved in the Pi-deficiency-triggered remodelling of RSA, for review see [118, 195]. The evidence is supported by previous finding that inhibition of ethylene biosynthesis with 2-aminoethoxyvinyl glycine (AVG) or ethylene perception with Ag+ restricted the low Pi-induced meristem exhaustion of the primary root [202]. Correspondingly, application of Ag+ was found to reduce the inhibition of primary root growth triggered by Pi deficiency. Moreover, Pi deficiency induced the formation of aerenchyma in adventitious roots, which is similarly induced by ethylene perception.
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At the transcriptional level, Lei et al. [203], using an Arabidopsis transgenic line that carries a LUC gene fused to the promoter of the high-affinity Pi transporter, AtPT2, showed that the transcription of AtPT2 is induced by Pi starvation. Using this marker line, the authors identified the Arabidopsis mutant etr1/hps2 (constitutive triple response 1/hyper-sensitive to Pi starvation2), which showed hyper-induction of the AtPT2::LUC gene by Pi deficiency. Interestingly, the expression of AtPT2 was partially blocked in ethylene insensitive 2 (ein2) mutants, but was enhanced in ethylene over producer1 (eto1) mutants. A similar expression pattern was observed for several other Pi starvation-induced (PSI) genes in the hps2 (negative regulator of ethylene response) and ein2 mutants, including high-affinity phosphate transporter, AtPT1 (Pht1;1); a non-coding transcript, At4; an APase, ACP5; a ribonuclease, Rxlink; and miR399d [204]. Enhanced transcription of PSI genes was also observed in the mutant hps3 and hps4, which are ETO1 alleles [205, 206]. ETO1 protein is a member of the broad complex/tramtrack/bric-a-brac (BTB) protein superfamily that participates in substrate recognition during ubiquitin-mediated protein degradation [204, 207]. ETO1 directly binds to the C-terminal of ACS5 and mediates its degradation. When ETO1 is mutated, it causes an overproduction of ethylene in young seedlings [208]. Application of 25 μM ACC to young Arabidopsis seedlings under high Pi conditions barely induces the expression of AtPT2. Under Pi deficiency, however, 0.5 μM ACC dramatically increases AtPT2 expression and induces ectopic root-hair development [203]. Thus, these results provide evidence that ethylene production and signaling is involved in the transcriptional responses of plants to Pi deficiency and primarily integrates with other Pi-deficiency-induced signaling pathways.
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The other signaling component involving increased transcription of purple acid phosphatase 10 (AtPAP10) by Pi starvation in the whole seedlings of hps3 and hps4 has been reported [205, 206]. AtPAP10 is a Pi starvation-induced APase (enzymes that scavenge Pi from organophosphate compounds) associated with the root surface. Functional analyses of atpap10 mutants suggest that AtPAP10 is important for plant tolerance to Pi starvation. However, the transcription of AtPAP10 does not significantly increase in ACC-treated seedlings or the constitutive triple response I (ctr1) mutant under Pi deficiency, nor does the accumulation of AtPAP10 proteins, which could suggest that ethylene has no effect on AtPAP10 transcription. More recently, Zhang et al. [209] have shown that positive regulation of AtPAP10 depends on sucrose and not ethylene. Moreover, they have also shown that ethylene does not affect AtPAP10 activity without sucrose, but the opposite is true. This suggests that ethylene could be a local but indirect signal for AtPAP10 activity. However, as discussed before, ethylene could be regulating other components of Pi starvation response at the transcriptional level. Song and Liu [204] have demonstrated that accumulation of anthocyanin is lower in hps2, hps3 and hps4 mutants under low Pi but increases in Pi-starved ein2 mutants. As mentioned before, accumulation of anthocyanins is an indicator of Pi-deficiency response in plants, thus ethylene could be a negative regulator of Pi-deficiency-induced anthocyanin accumulation probably through the regulation of genes involved in anthocyanin synthesis. Thus, ethylene likely participates at both the transcriptional and post-transcriptional levels, and this has implications on Pi starvation response in plants.
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The systemic response to P starvation is also carried out through a complex signaling network that involves other plant hormones [210, 211], sugars [212] and nitric oxide [213], collectively resulting in the alteration of carbohydrate distribution between roots and shoots. Amongst the plant hormones, other than ethylene, auxin likely plays a role in response to Pi starvation. However, ethylene likely exerts its influence through regulating auxin activity, as it has been associated with RSA remodelling [198]. Indeed, ethylene has been reported to interact with auxin and sugars, and changes in auxin transport and localization appear to be at least partially responsible for Pi stress-induced LR development [214]. Decreased sensitivity to CK and GA also appears to be at least partially responsible for Pi-stress-induced LR development [215]. Under low Pi, GA has been shown to repress Pi-induced root architecture changes [216]. Moreover, Pi-deficient plants were shown to accumulate DELLA proteins, the negative regulators of GA-induced root growth, which are modulated by auxin.
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As discussed before, amongst sugars, sucrose is key to Pi-deficiency response and appears to regulate ethylene activity. Amongst the TFs, phosphate starvation response proteins (e.g., OsPHR1, OsPHR2, PvPHR1, ZmPHR1 and TaPHR1), which bind the promoter sequences of low Pi-induced genes, and their regulator SMALL UBIQUITIN-LIKE MODIFIER1 [AtSIZ1; 217], a small ubiquitin-modified E3 ligase, and the downstream PHOSPHATE2 (PHO2), an E2 conjugase, are involved in Pi-deficiency-related transcriptional changes. Other TFs, including the bHLH, PTF1 (e.g., OsPTF1 and ZmPTF1) and MYB2P-1 (e.g., OsMYB2P1), MYB62, WRKY (e.g., WRKY75, WRKY6), bHLH32 and ZAT6 are also involved in the signaling network to regulate plant adaptation to P stress, for review see [218].
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Based on genetic analysis, two proteins, the P5 type ATPase encoded by PHOSPHATE DEFICIENCY RESPONSE2 (PDR2), and multicopper oxidase LOW PHOSPHATE ROOT1 (LPR1), were also previously shown to modulate Pi signaling in an endoplasmic-reticulum-localized pathway [219]. PDR2 is required for maintaining the levels of the root patterning gene, SCARECROW (SCR), and SHORT-ROOT protein (SHR) trafficking from stele into endodermis. PDR2 was proposed to act upstream of LPR1/LPR2 to adjust meristem activity. A recent study has shown that LPR1 is a ferroxidase [220]. Mutation of LPR1 reduces Fe3+ levels in the meristemic tissues of Pi-deficient plants. In contrast, increased levels of Fe3+ have been reported in pdr2 mutants leading to high production levels of reactive oxygen species (ROS). ROS signaling increases deposition of callose, which has been suggested to impair the trafficking of SHR, thus restricting root tip growth. Thus, PDR2 appears to modulate Pi-deficiency response by limiting Fe3+ accumulation in root tips.
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More recently, molecular mechanisms defining the phosphate signaling pathway showed that phosphate uptake 1 (Pup1)-specific protein kinase gene, named phosphorus-starvation tolerance 1 (PSTOL1), was confirmed to be involved in regulating root growth and architecture during early stages of rice growth [221]. Allele-specific markers for this gene have been reported recently [222]. Interestingly, OsPSTOL1 is located within the Kasalath-specific INDEL region and is absent from the rice variety Nipponbare reference genome. Thus, the configuration of the functional mechanism of PSTOL1 is still elusive. We speculate that PSTOL1 could be a local sensor of Pi starvation which transduces signals for sucrose or ethylene biosynthesis or both. The interplay of sucrose accumulation and ethylene biosynthesis is apparently the hallmark of Pi starvation response in plants.
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The post-transcriptional regulation as well as long-distance signaling is carried out by microRNAs. As mentioned before, miR399, which is regulated by PHR1, a conserved MYB TF, maintains P homeostasis by regulating P transporter PHO2 [223]. In tomato, overexpression of Arabidopsis\n\n miR399 increases both the Pi accumulation and secretion of acid phosphatase and protons in the roots [223]. Thus, miR399 is important for Pi acquisition, and could be acting downstream of sucrose and probably ethylene. Overexpression of miR399 in Arabidopsis also increases P uptake and allocation to the shoot. Moreover, P remobilization from older leaves to young leaves is defective in Arabidopsis miR399 transgenic lines [224]. This suggests that miR399 is important for allocation and remobilization of P. The targets of miR399 include a ubiquitin-conjugating E2 enzyme (UBC24) encoded by PHO2, which is upregulated under P-sufficient conditions and downregulated in P-starved plant roots. Homologues of PHO2/UBC24 have a conserved structure in many species, and their 5′ UTR regions possess multiple miR399-complementary sequences. Thus, the regulatory mechanism of miR399-PHO2 complex is evolutionarily conserved in angiosperms, making it a potential target for improving P nutrition efficiency in plants.
\n
Strigolactones (SL) have also been shown to be induced by low Pi in many species, including tomato, Arabidopsis, pea and rice [225–229]. Strigolactones are terpenoid lactones that function as either endogenous hormones that control plant development or as components of root exudates that promote symbiotic interactions between plants and soil microbes. The production and exudation of SLs may depend on whether the plant is arbuscular mycorrhizal fungi (AMF)-compatible host or an arbuscular mycorrhizal symbiosis (AMS) for Pi and N uptake. A well-known synthetic SL, GR24, apparently increases LR formation under low Pi or decreases LR formation under sufficient Pi. In addition, SL biosynthesis (more axillary growth; max4-1) and signaling (max2-1) mutants have reduced number of root hairs under low Pi condition at the early stages of seedling development. This suggests that SLs mediate plant responses to low Pi; however, the mechanism by which SL exudation affects root growth is not fully understood.
\n
In conclusion, although the molecular components of P stress signaling in plants have been fairly documented, the overall pathway is still less understood and requires further investigation. Nonetheless, the recent developments in whole genome sequencing technologies provide hope for more studies on plants with better P acquisition and utilization. Successes in QTL analysis have also set a stage for subsequent studies. Besides the success story of PSTOL1 in rice, QTL analysis in common bean has shown the importance of basal roots and adventitious roots for P acquisition [230–232]. Another study by Yan et al. [233] identified a large number of QTLs for Hþ exudation, root-hair density and length, associated with P efficiency. Additionally, QTLs for root traits related to P efficiency have also been identified in soybean [234, 235]. Moreover, QTLs controlling P deficiency tolerance were mapped by Zhang et al. [344] using 152 RILs derived from a cross between P-stress-tolerant and P-stress-sensitive parents. Thus, future studies will build on these present discoveries to facilitate genetic improvement for Pi-deficiency tolerance.
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7.2. Advances in plant tolerance to nutrient toxicities
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Metal toxicity is an important factor limiting the growth of plants in many environments. Some metals, such as copper and zinc, are micronutrients at low concentrations and become toxic at higher levels, whereas others (e.g., aluminium, iron, cadmium, chromium and lead) are well known for their toxicity [236]. These elements can be highly phytotoxic and seriously impair plant root growth. However, some crops are able to tolerate toxic environments, without significant display of toxicity symptoms. Three main strategies are employed by such plants to manage toxic soil compounds: (1) Producing root exudates that bind and neutralize the toxin in the rhizosphere, (2) actively transport the compound into the root, but neutralizing and sequestering it in vacuoles for safe accumulation or eliminating it through exudation and (3) excluding the toxic elements by preventing entry into the plant tissues. For the purpose of this chapter, we will focus on aluminium and iron toxicities as these elements have been frequently reported as major constraints in the production of economically important crops.
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7.2.1. Plant tolerance to aluminium toxicity
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Aluminium (Al) is a light metal that makes up 7% of the Earth’s crust and is the third most abundant element after oxygen and silicon. Aluminium toxicity is one of the major constraints to crop productivity worldwide, especially in the acid soils of the tropics and subtropics that comprise almost 50% of all non-irrigated arable land in those regions [118, 237]. The soil pH has a crucial role for Al toxicity to occur, by affecting both solubility and the ability of plant roots to absorb Al. Al solubilizes into its toxic form (Al3+) when the soil pH drops to 5.5 or less, and is most severe in solutions of low ionic strength and low cation concentrations. Al3+ is taken up by plants through diffusion [238], and toxic concentrations of >12 µM are detrimental to root growth. Possible exceptions of Al(OH)3\n\n 4− toxicity at higher pH values have also been reported [239].
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The initial effects of Al3+ toxicity on the roots include rapid inhibition of cell division and a reduction in root apical cell expansion and elongation. Consequently, plants develop stubby and brittle roots with swollen malformed root tips. Moreover, lateral root initiation and outgrowth are also inhibited. Root-hair malformation is often reported, and nutrient (mainly P, K, Ca and Mg) and water uptake capacity is impaired [238]. Plant responses in the shoots include reduced stomatal opening, chlorosis, foliar necrosis and reduced photosynthetic activity.
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Plant tolerance to aluminium toxicity occurs through (1) external avoidance, which involves root secretion of organic acids to chelate Al3+ in the rhizosphere, limiting its diffusion into the roots [240], and (2) true or internal tolerance, which involves regulation of Al3+ uptake, and organic acid chelation and sequestration of aluminium bound substrates [241]. In rice, the latter is the main tolerance mechanism, and is apparently associated with the differential expression and transport properties of membrane transporters, e.g., NRAMP Al\n \n 3+\n \n\n transporter 1 (NRAT1) [242]. Most other plant species also vary significantly in these mechanisms; however, there are some tolerance mechanisms that are largely shared. Cereal crops, such as wheat, barley, sorghum (Sorghumbicolor L.) and oat were reported to have simple genetic mechanisms of Al tolerance, whereas rice and maize (Zea mays L.) have over time developed complicated inheritance controlled by numerous genes/loci involved [118, 243].
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Genetic control of organic acid exudation either rests on the Multidrug and Toxin Efflux (MATE) family encoding a citrate transporter or on the membrane localized Al3+-activated malate transporters (ALMT). Several transporters in these families, including HvAACT1 in barley [244], TaALMT1 and TaMATE1 in wheat [245] and ZmMATE1 and ZmMATE2 in maize [246] are responsible for organic acid exudation and Al tolerance. Specific markers for HvAACT1 and the MATE gene, HvMATE-21, have been developed and can be used to differentiate tolerant and sensitive barley cultivars. Differences amongst these transporters however exist. For instance, TaALMT1 encodes a malate transporter on chromosome 4D and is constitutively expressed on root apices, whereas TaMATE1 reportedly responds to Al stress based on citrate efflux. ZmMATE1 and ZmMATE2 co-segregate with two major Al-tolerance QTLs [247]. ZmMATE1 was shown to be induced by Al and enhances Al tolerance, whereas ZmMATE2 did not respond to Al [246], suggesting variability in their roles. In sorghum, Al tolerance is controlled by SbMATE, encoded by a major Al-tolerant locus AltSB on chromosome 3 [248]. In Arabidopsis , two genes were reportedly responsible for Al tolerance: AtALMT1 that also encodes a malate transporter responsible for malate efflux on chromosome 1 [249] and AtMATE that encodes an Al-activated citrate transporter [389]. These two genes function independently, but both are regulated by the C2H2-type zinc finger transcription factor STOP1 [250], which is also reportedly induced by with low pH tolerance [366]. In rye, ScALMT1, which is mainly expressed in the root apex and upregulated by Al, co-segregates with the Alt4 locus on chromosome 7RS [367]. Another candidate gene ScAACT1 on chromosome 7RS was mapped to 25 cM from ScALMT1 [251].
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At the transcriptome level, two genes, SENSITIVE TO ALUMINUM RHIZOTOXICITY1 and 2 (STAR1 and 2), which encode the nuclear binding domain and the transmembrane domain, respectively, of an ABC transporter, with specificity for uridine diphosphate (UDP) glucose, are upregulated following root exposure to Al3+ [252]. Both STAR genes were previously reported to be upregulated by the constitutively expressed rice root ALUMINUM RESISTANT TRANSCRIPTION FACTOR1 (ART1), which also upregulates several other genes implicated in different aluminium tolerance mechanisms [253]. More recently, ASR5 was reported to act as a key TF that is essential for Al-responsive STAR1 and other Al response genes [254]. Rice homologues, which encode α-expansin (e.g., EXPA10), belong to this family of TFs, and have been implicated in the regulation of root elongation and cell wall elasticity. The members of EXPA10 decrease cell wall extension potential when exposed to Al3+ [255] and are downregulated during Al3+ stress. The functions of STAR1, STAR2/ALS3 and ALS1 in Al tolerance are fairly conserved and ubiquitous in monocot and dicot species. However, these genes are differentially expressed between species. For instance, the expression and induction levels of these genes in response to Al3+ stress are higher in the Al-tolerant species of rice than in the Al-sensitive species of Arabidopsis , suggesting that Al-tolerant species may require increased expression of these conserved Al-tolerance genes to overcome Al3+ stress [256]. The same authors show that Tartary buckwheat shows high expression of the Al-tolerance gene homologues under Al3+ stress. Al-tolerance in buckwheat is evolutionarily closer to Arabidopsis than rice, suggesting that buckwheat could have rapidly evolved higher expression of Al-tolerance genes to detoxify Al3+ than Arabidopsis . In addition, the gene duplication of ART1/STOP1, STAR1 and ALS1 has been suggested to play a significant role in Al tolerance. This is consistent with the previous findings that duplication of key genes responsible for metal translocation and detoxification in Arabidopsis halleri facilitates hyper-accumulation of zinc/cadmium [257]. However, further functional analysis by creating knockdown or knockout mutants will be necessary to provide additional insights into the role of each homologous gene in Al detoxification and accumulation in buckwheat.
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An Arabidopsis cell-wall-associated putative endochitinase, CHITINASE A (AtCHIA), likely involved in modulating cell wall extension by regulating chitin levels, has also been suggested to play a role in Al tolerance [258]. Another signal of Al3+-induced cellular response is the induction of 1,3-β-d-glucan synthase, which leads to the accumulation of callose in root apices, especially in endodermal and cortical cell walls [259, 260]. This callose deposition is suggested to be an inhibitory process that may block symplastic and apoplastic flows. Whether callose deposition represents Al3+-induced injury or a defence response to block further Al3+ binding and movement remains to be confirmed.
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In Arabidopsis, the ethylene receptor gene ETHYLENE RECEPTOR1 (ETR1) and the ethylene signal transducer ETHYLENE INSENSITIVE2 (EIN2) were found to be important for Al3+-induced inhibition of root elongation [261]. These genes apparently regulate Al3+-induced upregulation of the Arabidopsis ethylene synthesis genes 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE2, 6, and 8 and 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID OXIDASE1 and 2. Moreover, ET was recently shown to negatively regulate aluminium-induced malate efflux from wheat roots and tobacco cells transformed with TaALMT1 [262], which suggests that ethylene could be a negative regulator of root secretion of organic acids. The upregulation of auxin transporters PIN FORMED2 (PIN2) and auxin influx carrier AUXIN RESISTANT1 (AUX1), which regulate auxin distribution, is associated with the regulation of root elongation in Arabidopsis plants [263]. AUX1 and PIN2 are apparently disrupted by ethylene signal that alters auxin distribution and transport in the roots. He et al. [264] suggests that auxin could be involved in aluminium-induced efflux of malic acid acting on anion channels. Thus, auxin/IAA transport could be a target for Al3+ toxicity tolerance if the modulation by ET is attenuated. However, considering several phytohormonal changes that occur during Al stress, molecular mechanisms associated with their interplay will require further elucidation. Recent evidence that microRNAs are involved in Al stress tolerance [265] also provides new insights into understanding the mechanism of Al3+ tolerance in plants.
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Overall, we expect that major advances in understanding physiological and molecular basis for Al tolerance will happen in the near future, considering that the pace at which new genes are being discovered has improved with new sequencing technologies. The future challenge for studying Al tolerance is the identification of new tolerance mechanisms. The discovery of the key molecular regulators, e.g., ASR5, which was recently shown to mediate Al-responsive gene expression to provide Al tolerance in rice, is an indication that several other mechanism of Al tolerance exist in plants. The blocking of Al3+ cell wall binding sites in rice may be one of the major mechanisms of aluminium tolerance that will need further investigation. Studies on barley, wheat and maize have shown variation in gene expression associated with variation in gene sequence, which would require further investigation to understand the regulatory networks affected by this sequence polymorphisms.
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7.2.2. Advances in plant tolerance to iron toxicity
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The problem of iron toxicity occurs in most wetland rice growing areas of the world, primarily in flooded acidic soils, inland and coastal swamps. Some of the irrigated lands in South and Southeast Asia, Africa and South America are affected [266]. In India alone, about 11.7 million hectares of land are affected by iron toxicity. In Burkina Faso, 300 ha of ferrous iron intoxicated soils were abandoned in the Valley du Kou in 1986, most of which remained uncultivated to date [267]. Iron toxicity is also becoming a major rice yield limiting factor in East Africa, including lowland rice cultivation areas of Uganda [268]. Yield losses in the range of 10% to 100% have been reported [266]. Moreover, toxicity at seedling and early vegetative stages can strongly affect plant growth and hinder development, and can result in complete crop failure.
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Three major adaptation mechanisms are generally reported for Fe-toxicity tolerance. The details by which rice plants execute these processes and their molecular components are not yet fully understood, but there are some clues from various studies on rice and other plant species. For instance, plant tolerance by root oxidizing power is mediated by diffusion of molecular oxygen from the shoots to the roots through aerenchyma tissue and its subsequent release in the rhizosphere. Oxidation of Fe2+ in the rhizosphere results in the precipitation of insoluble iron oxides at the root surface, forming iron plaques. These iron plaques not only reduce Fe2+ concentration in the soil solution, but also form a physical barrier against further influx of Fe2+ into the roots.
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Plant tolerance by retention of iron in the root or shoot involves compartmentalization. Nicotianamine (NA), Fe-NA complex transporters, VIT proteins, FPN2-like proteins, MIT- and \n PIC1\n\n -like proteins, organic acids, ferritins, Fe-sulphur and other heme proteins that can sequester Fe are all potential candidates for plant tolerance to excess iron through regulated storage and compartmentalization (Figure 6).
\n
Figure 6.
Iron transport in rice. Fe is taken up into the symplast by transporters in the epidermis (OsIRT1, OsNRAMP, OsZIPL1 and OsYSLs). Proteins encoded by bHLH, IRUNLP1 and IRT2 likely regulate the activities of the above transporters. Radial oxygen loss into the rhizosphere through aerenchyma cells detoxifies part of the excess iron forming insoluble Fe3+ at the root surfaces, a process referred to as exclusion. Excess Fe2+ travels through the symplastic space to the vasculature, bypassing the waxy Casparian strip on the endodermis. Prior to reaching the xylem, excess iron is retained in the root cell vacuoles, mitochondria and probably detoxified by organic acids within the root cells. Transport into the xylem is mediated by putative chelate effluxers: FRDL1, OsYSLs, TOM1, OsIRT1, PEZ1 and FPN1. In the xylem, iron is carried to the shoot through the transpiration stream either in the form of Fe3+ or in both Fe3+ and Fe2+ forms, and unloaded into the shoot, most likely by YSLs, FRO1 and OsIRT1 proteins. Within the phloem, the rate at which NA, DMA and ITP are synthesized, the kinetic stability of the complexes formed and the oxido-reduction system likely determines the iron speciation. Enzymes involved in NA, DMA and ITP synthesis, including OsIRO2, OsNAS1, NAAT1 and DMAS1, likely play a significant role in determining iron loading into the phloem. Genes encoding for putative iron effluxers from the phloem to storage organs (VIT, OsNRAMP, HMA3, MTP1, ENA, MIT1, ATM1) are co-regulated with IREG2/FPN2 and YSLs to limit potentially toxic iron in the cytosol, by compartmentalizing in the vacuoles, mitochondria, chloroplast and other non-characterized intracellular vesicles. In the chloroplasts, Fe excess probably promotes NO production. NO is probably involved in activation of the transcription factor (TF) cascades responsible for the regulation of Fe uptake, homeostasis and for the tuning of cellular metabolism, including increased synthesis of ferritins and betalains in chloroplasts and frataxins in the mitochondria. Because NO also triggers the synthesis of ROS, heme biosynthesis likely occurs to compartmentalize excess iron and to limit NO production. Alongside heme biosynthesis, the potent antioxidant system involving SOD and APX probably scavenge and detoxify the excess ROS. Also presented are targets of iron utilization, which could reduce iron overload. This includes synthesis of ferrochelatase (FC) for heme biosynthesis, mitochondrial iron-sulphur cluster (ISC) and plastid-localized sulphur utilization factors (SUF).
\n
In Arabidopsis , apoplasmic Fe is mostly found within the stele [269], suggesting that compartmentalization within the stele could restrict excess Fe from reaching the shoot during transportation towards the aerial parts. Fe2+ decreases could also occur in association with an alkalization of apoplastic pH, which reduces Fe2+ mobility and chemical stability [269]. Alkalization has been reported to be modulated by ethylene [270], suggesting additional role of ethylene in regulating Fe2+ besides its role in aerenchyma formation. Tissue tolerance of Fe toxicity is mediated by detoxification of free radicals. In rice, expression of several genes involved in oxidative stress control, including peroxidases, glutathione transferase (GST) and cytochromes, was upregulated in roots and shoots in response to excess Fe [271]. Similar trends were observed at the protein and enzymatic activity levels of the same genes. Excess iron was reported to induce the activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in the leaf sheath and laminae, respectively, in a tolerant variety from Oryza glaberrima [272]. The activity of glutathione reductase and peroxidase (POD) was also reported to increase in rice leaf segments exposed to excess iron [273]. Fang et al. [274] also showed that lipid peroxidation resulting from Fe toxicity was inhibited by free radical scavengers such as mannitol and GSH. Moreover, the differential expression of anti-oxidant enzyme activities (SOD, APX, CAT, GR and DHR) was observed between rice varieties contrasting in tolerance of Fe toxicity [275].
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Several genetic studies also reflect that iron toxicity tolerance is a complex quantitative trait controlled by a large number of rather small effect quantitative trait loci (QTLs), indicating the involvement of multiple tolerance mechanisms. For instance, Wu et al. [276] identified QTLs for leaf bronzing and shoot dry weight on chromosome 1 and 8, explaining 10–32% of the phenotypic variation. Interestingly, QTLs associated with enzymatic activity of anti-oxidants in rice leaves were detected in the same region [277]. Similarly, Fukuda et al. [278] detected a region on chromosome 3 responsible for high shoot iron content in a susceptible variety, which co-localize with the QTL previously identified by Shimizu et al. [279] for the same trait. Co-localization of most of these QTLs was captured in an integrative genetic map reflecting mapping studies from different conditions of Fe toxicity [277], which substantiates on recurrent chromosomal regions identified in several QTL studies.
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A major limitation of iron toxicity tolerance studies, however, is that most of the QTLs associated with iron toxicity tolerance have not been furthered to cloning of tolerance genes. It is thus critical to devote some effort to fine-map the few, but consistent QTLs mentioned herein in order to increase precision and accelerate candidate gene identification. Subsequently, functional validation of several genes identified in microarray studies will need to be explored. Exploring allelic variation of these genes in contrasting genotypes and evaluating the promising alleles in well designed and efficient phenotyping experiments would provide a basis for their use in marker-assisted breeding (MAB) for Fe-toxicity tolerance.
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8. Conclusions and perspectives
\n
In this chapter, we have attempted to present the recent advances in crop tolerance to abiotic stresses. Various strategies used by plants to counteract stress, and some success in identifying genomic regions associated with plant tolerance is presented. Interestingly, plants have evolved common regulatory networks in response to abiotic stresses. For instance, drought, salt and cold stress induce calcium influx to activate the downstream second messengers to yield different or similar responses. Calcium influx channels at the membrane (e.g., the recently reported hyper-osmolality induced [Ca\n \n 2+\n \n\n ] increases 1 (OSCA1) from Arabidopsis thaliana that is gated by hyper-osmotic stress [280]) act in concert with the membrane-located NADPH-oxidase Respiratory burst oxidase Homolog (RboH), generating apoplastic ROS. Intracellular transduction is conveyed by calcium-binding proteins (e.g., CBLs/CIPKs, CDPKs and calcineurins), a MAP-Kinase cascade and phytohormones (e.g., ABA, ET, JA and SA), which apparently act as integrators of early signals. Depending on the relative temporal patterns of these upstream signals, the activity of TFs and their interacting proteins will decipher specific combinations of genes required to be expressed to boost enzymatic or protein reaction levels necessary to counter the stress perceived. These proteins largely contribute to adaptive response in most plants, e.g., production of compatible osmolytes that helps to reinstall turgidity during drought and synthesis of LEA proteins that prevent protein precipitation. Other examples include chelation/sequestering of ions into cellular compartments in response to toxic elements, induction of anti-oxidative enzymes, induction of molecular chaperones and adaptive regulation of plant hormones. These adaptive strategies and the molecular components involved provide potential molecular genetic targets for enhancing abiotic resistance in crops.
\n
However, many challenges still lie ahead. For example, the regulation of signaling cascades, especially how plants can discriminate the signaling components, and even their specific combinations, to activate specific downstream biological processes for a given stress. A frequent manifestation has been the case of ethylene controversial role in abiotic stress response. Whether the negative regulations associated with ethylene represent a plant strategic mechanism to prime the subsequent useful reaction remains to be confirmed. Also, temporal and specific differences in activation of upstream signaling components will need to be explored to help in identifying molecular components essentially required to counter a given stress. Moreover, the specific downstream components for which much of the studies have been conducted, e.g. transcription factors, transmembrane proteins, transporters, enzymes for osmolyte biosynthesis, hormonal regulators, ROS scavengers and other traits that have been shown to play major roles in plant response to stress, will need classification according to their aptitude and functional significance in response to a given abiotic stress. Morpho-physiological traits associated with stress tolerance would also substantially reinforce the successes in molecular biology if addressed to a greater extent. The use of models for predicting gene effects, particularly when combining multiple traits, will also find greater application in dissecting G × E interactions and will help breeders to improve target varieties. Thus, there is need to integrate molecular tools with precise high-throughput phenotyping and biochemical analysis to confirm the consistency of various molecular findings, and to realize the full benefits of molecular biology in selecting genotypes that are stably tolerant under a given stress, considering the interaction with various environments. Here, we emphasize stresses that have been commonly reported in literature, which would provide a basis for understanding other minor stresses. We also refer to the chapter on biotic stresses and the numerous interactions in signaling pathways and expressions of resistance and tolerance on molecular level towards abiotic and biotic stress in plants. Additional background information can also be found in excellent reviews and references therein.
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Acknowledgments
This publication was supported by Erfurt University of Applied Sciences.
\n',keywords:"abiotic stress, climate change, crop improvement, transcription, regulatory proteins",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/51235.pdf",chapterXML:"https://mts.intechopen.com/source/xml/51235.xml",downloadPdfUrl:"/chapter/pdf-download/51235",previewPdfUrl:"/chapter/pdf-preview/51235",totalDownloads:4336,totalViews:2102,totalCrossrefCites:21,totalDimensionsCites:45,totalAltmetricsMentions:0,impactScore:14,impactScorePercentile:99,impactScoreQuartile:4,hasAltmetrics:0,dateSubmitted:"May 22nd 2015",dateReviewed:"May 23rd 2016",datePrePublished:null,datePublished:"July 14th 2016",dateFinished:"June 17th 2016",readingETA:"0",abstract:"During the last 50 years, it has been shown that abiotic stresses influence plant growth and crop production greatly, and crop yields have evidently stagnated or decreased in economically important crops, where only high inputs assure high yields. The recent manifesting effects of climate change are considered to have aggravated the negative effects of abiotic stresses on plant productivity. On the other hand, the complexity of plant mechanisms controlling important traits and the limited availability of germplasm for tolerance to certain stresses have restricted genetic advances in major crops for increased yields or for improved other traits. However, some level of success has been achieved in understanding crop tolerance to abiotic stresses; for instance, identification of abscisic acid (ABA) receptors (e.g., ABA-responsive element (ABRE) binding protein/ABRE binding factor (AREB/ABF) transcription factors), and other regulons (e.g., WRKYs, MYB/MYCs, NACs, HSFs, bZIPs and nuclear factor-Y (NF-Y)), has shown potential promise to improve plant tolerance to abiotic stresses. Apart from these major regulons, studies on the post-transcriptional regulation of stress-responsive genes have provided additional opportunities for addressing the molecular basis of cellular stress responses in plants. This chapter focuses on the progress in the study of plant tolerance to abiotic stresses, and describes the major tolerance pathways and implicated signaling factors that have been identified, so far. To link basic and applied research, genes and proteins that play functional roles in mitigating abiotic stress damage are summarized and discussed.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/51235",risUrl:"/chapter/ris/51235",book:{id:"5098",slug:"plant-genomics"},signatures:"Geoffrey Onaga and Kerstin Wydra",authors:[{id:"176967",title:"Prof.",name:"Kerstin",middleName:null,surname:"Wydra",fullName:"Kerstin Wydra",slug:"kerstin-wydra",email:"kerstin.wydra@fh-erfurt.de",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Charité",institutionURL:null,country:{name:"Germany"}}},{id:"176968",title:"Dr.",name:"Geoffrey",middleName:null,surname:"Onaga",fullName:"Geoffrey Onaga",slug:"geoffrey-onaga",email:"geoffrey.onaga@agr.uni-goettingen.de",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Advances in plant tolerance to drought",level:"1"},{id:"sec_3",title:"3. Advances in plant tolerance to heat stress",level:"1"},{id:"sec_4",title:"4. Advances in plant tolerance to cold stress",level:"1"},{id:"sec_5",title:"5. Advances in plant tolerance to salinity",level:"1"},{id:"sec_6",title:"6. Advances in plant tolerance to submergence/flooding",level:"1"},{id:"sec_7",title:"7. Advances in plant tolerance to nutrient imbalances",level:"1"},{id:"sec_7_2",title:"7.1. Tolerance to nutrient deficiency",level:"2"},{id:"sec_7_3",title:"7.1.1. Plant tolerance to nitrogen deficiency",level:"3"},{id:"sec_8_3",title:"7.1.2. Plant tolerance to phosphorus deficiency",level:"3"},{id:"sec_10_2",title:"7.2. Advances in plant tolerance to nutrient toxicities",level:"2"},{id:"sec_10_3",title:"7.2.1. Plant tolerance to aluminium toxicity",level:"3"},{id:"sec_11_3",title:"7.2.2. Advances in plant tolerance to iron toxicity",level:"3"},{id:"sec_14",title:"8. Conclusions and perspectives",level:"1"},{id:"sec_15",title:"Acknowledgments",level:"1"}],chapterReferences:[{id:"B1",body:'\n Acquaah G. Principles of plant genetics and breeding. 2nd ed. Oxford, UK: Wiley; 2012. 20 pp.\n '},{id:"B2",body:'\n Dos Reis SP, Lima AM, de Souza CRB. Recent molecular advances on downstream plant responses to abiotic stress. International Journal of Molecular Sciences. 2012;13:8628–8647. DOI: 10.3390/ijms13078628\n '},{id:"B3",body:'\n Flowers TJ, Yeo AR. Breeding for salinity resistance in crop plants—where next? Australian Journal of Plant Physiology. 1995;22:875–884. DOI: 10.1071/PP9950875\n '},{id:"B4",body:'\n Colmer TD, Pedersen O. Underwater photosynthesis and respiration in leaves of submerged wetland plants: gas films improve CO2 and O2 exchange. New Phytologist. 2008;177:918–926. DOI: 10.1111/j.1469-8137.2007.02318.x\n '},{id:"B5",body:'\n Mittler R. Abiotic stress, the field environment and stress combination. Trends Plant Science. 2006;11:15–19. DOI: 10.1016/j.tplants.2005.11.002\n '},{id:"B6",body:'\n Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, Shinozaki K. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Current Opinion in Plant Biology. 2006;9:436–442. DOI: 10.1016/j.pbi.2006.05.014\n '},{id:"B7",body:'\n Chinnusamy V, Schumaker K, Zhu JK. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signaling in plants. Journal of Experimental Botany. 2004;55:225–236. DOI: 10.1093/jxb/erh005\n '},{id:"B8",body:'\n Krasensky J, Jonak C. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany. 2012;63:1593–1608. DOI: 10.1093/jxb/err460\n '},{id:"B9",body:'\n Ramakrishna A, Ravishankar GA. Influence of abiotic stress singles on secondary metabolites in plants. Plant Signaling Behavior. 2011;6:1720–1731. DOI: 10.4161/psb.6.11.17613\n '},{id:"B10",body:'\n Zhu QH, Zheng XM, Luo JC, Gaut BS, Ge S. Multilocus analysis of nucleotide variation of Oryza sativa and its wild relatives: severe bottleneck during domestication of rice. Molecular Biology and Evolution. 2007;24:875–888. DOI: 10.1093/molbev/msm005\n '},{id:"B11",body:'\n Buckler ES, Thornsberry JM, Kresovich S. Molecular diversity, structure and domestication of grasses. Genetical Research. 2001;77:213–218. DOI: 10.1017/S0016672301005158\n '},{id:"B12",body:'\n Haudry A, Cenci A, Ravel C, Bataillon T, Brunel D, Poncet C, Hochu I, Poirier S, Santoni S, Glémin S, David J. Grinding up wheat: a massive loss of nucleotide diversity since domestication. Molecular Biology Evolution. 2007;24:1506–1517. DOI: 10.1093/molbev/msm077\n '},{id:"B13",body:'\n Kim JY, Mahé AB, rangeon J, Prioul JL. A maize vacuolar invertase, IVR2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression. Plant Physiology. 2000;124:71–84. DOI: 10.1104/pp.124.1.71\n '},{id:"B14",body:'\n Liu F, Jensen CR, Andersen MN. Drought stress effect on carbohydrate concentration in soybean leaves and pods during early reproductive development: its implication in altering pod set. Field Crops Research. 2004;86(1):1–13. DOI: 10.1016/S0378-4290(03)00165-5\n '},{id:"B15",body:'\n Hirayama T, Shinozaki K. Research on plant abiotic stress responses in the post genome era: past, present and future. The Plant Journal. 2010;61(6):1041–1052. DOI: 10.1111/j.1365-313X.2010.04124.x\n '},{id:"B16",body:'\n Umezawa T, Nakashima K, Miyakawa T, Kuromori T, Tanokura M, Shinozaki K, Yamaguchi-Shinozaki K. Molecular basis of the core regulatory network in ABA responses: sensing, signaling and transport. Plant & Cell Physiology. 2010;51:1821–1839. DOI: 10.1093/pcp/pcq156\n '},{id:"B17",body:'\n Boursiac Y, Leran S, Corratge-Faillie C, Gojon A, Krouk G, Lacombe B. ABA transport and transporters. Trends in Plant Science 2013;18:325–333. DOI: http://dx.doi.org/10.1016/j.tplants.2013.01.007\n '},{id:"B18",body:'\n Golldack D, Li C, Mohan H, Probst N. Tolerance to drought and salt stress in plants: unraveling the signaling networks. Frontiers in Plant Science. 2014;5:151. DOI: 10.3389/fpls.2014.00151\n '},{id:"B19",body:'\n Vlad F, Rubio S, Rodrigues A, Sirichandra C, Belin C, Robert N, Leung J, Rodriguez PL, Laurière C, Merlot S. Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis. The Plant Cell 2009;21:3170–3184. DOI: 10.1105/tpc.109.069179\n '},{id:"B20",body:'\n Campo S, Baldrich P, Messeguer J, Lalanne E, Coca M, San Segundo B. Overexpression of a calcium-dependent protein kinase confers salt and drought tolerance in rice by preventing membrane lipid peroxidation. Plant Physiology. 2014;165:688–704. DOI: 10.1104/pp.113.230268\n '},{id:"B21",body:'\n Ouyang SQ, Liu YF, Liu P, Lei G, He SJ, Ma B, Zhang WK, Zhang JS, Chen SY. Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants. The Plant Journal. 2010;62:316–329. DOI: 10.1111/j.1365-313X.2010.04146.x\n '},{id:"B22",body:'\n Osakabe Y, Yamaguchi-Shinozaki K, Shinozaki K, Lam-Son Phan T. ABA control of plant macro element membrane transport systems in response to water deficit and high salinity. New Phytologist. 2014;202:35–49. DOI: 10.1111/nph.12613\n '},{id:"B23",body:'\n Xu C, Jing R, Mao X, Jia X, Chang X. A wheat (Triticum aestivum) protein phosphatase 2A catalytic subunit gene provides enhanced drought tolerance in tobacco. Annals of Botany. 2007;99:439–450. DOI: 10.1093/aob/mcl285\n '},{id:"B24",body:'\n Banerjee A, Roychoudhury A. WRKY proteins: signaling and regulation of expression during abiotic stress responses. The Scientific World Journal. 2015;2015:807560. DOI: 10.1155/2015/807560\n '},{id:"B25",body:'\n Ding Z, Li S, An X, Liu X, Qin H, Wang D. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana. Journal of Genetics and Genomics. 2009;36:17–29. DOI: 10.1016/S1673-8527(09)60003-5\n '},{id:"B26",body:'\n Rushton DL, Tripathi P, Rabara RC, Lin J, Ringler P, Boken AK, Langum TJ, Smidt L, Boomsma DD, Emme NJ, Chen X, Finer JJ, Shen QJ, Rushton PJ. WRKY transcription factors: key components in abscisic acid signaling. Plant Biotechnology Journal. 2012;10:2–11. DOI: 10.1111/j.1467-7652.2011\n '},{id:"B27",body:'\n Zhang G, Chen M, Li L, Xu Z, Chen X, Guo J, Ma Y. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. Journal of Experimental Botany. 2009;60:3781–3796. DOI: 10.1093/jxb/erp214\n '},{id:"B28",body:'\n Liu S, Wang X, Wang H, Xin H, Yang X, Yan J Li J, Tran LS, Shinozaki K, Yamaguchi-Shinozaki K, Qin F. Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L. PLoS Genetics. 2013;9(9):e1003790. DOI: 10.1371/journal.pgen.1003790\n '},{id:"B29",body:'\n Ji X, Liu G, Liu Y, Zheng L, Nie X, Wang Y. The bZIP protein from Tamarix hispida, ThbZIP1, is ACGT elements binding factor that enhances abiotic stress signaling in transgenic Arabidopsis. BMC Plant Biology. 2013;13. DOI: 10.1186/1471-2229-13-151\n '},{id:"B30",body:'\n Ying S, Zhang DF, Fu J, Shi YS, Song YC, Wang TY, and Li Y. Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis. Planta. 2012;235:253–266. DOI: 10.1007/s00425-011-1496-7\n '},{id:"B31",body:'\n Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran LS, Yamaguchi-Shinozaki K, Shinozaki K. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. The Plant Journal. 2004;39:863–876. DOI: 10.1111/j.1365-313X.2004.02171.x\n '},{id:"B32",body:'\n Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress 1 promoter. The Plant Cell 2004;16:2481–2498. DOI: 10.1105/tpc.104.022699\n '},{id:"B33",body:'\n Jeong JS, Kim YS, Baek KH, Jung H, Ha SH, Do Choi Y, Kim M, Reuzeau C, Kim JK. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiology. 2010;153:185–197. DOI: 10.1104/pp.110.154773\n '},{id:"B34",body:'\n Jeong JS, Kim YS, Redillas MC, Jang G, Jung H, Bang SW Choi YD, Ha SH, Reuzeau C, Kim JK. OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field. Plant Biotechnology Journal. 2013;11:101–114. DOI: 10.1111/pbi.12011\n '},{id:"B35",body:'\n Palmeros-Suárez PA, Massange-Sánchez JA, Martínez-Gallardoa NA, Montero-Vargas JM, Gómez-Leyva JF, Délano-Frier JP. The overexpression of an Amaranthus hypochondriacus NF-YC gene modifies growth and confers water deficit stress resistance in Arabidopsis. Plant Science. 2015;240:25–40. DOI: 10.1016/j.plantsci.2015.08.010\n '},{id:"B36",body:'\n Chen M, Zhao Y, Zhuo C, Lu S, Guo Z. Overexpression of a NF-YC transcription factor from Bermuda grass confers tolerance to drought and salinity in transgenic rice. Plant Biotechnology Journal. 2015;13:482–491. DOI: 10.1111/pbi.12270\n '},{id:"B37",body:'\n Fang Y, Liao K, Du H, Xu Y, Song H, Li X, Xiong LA. Stress-responsive NAC transcription factor SNAC3 confers heat and drought tolerance through modulation of reactive oxygen species in rice. Journal of Experimental Botany. 2015;25:3472–3490. DOI: 10.1093/jxb/erv386\n '},{id:"B38",body:'\n Djafi N, Vergnolle C, Cantrel C, Wietrzyñski W, Delage E, Cochet F Puyaubert J, Soubigou-Taconnat L, Gey D, Collin S, Balzergue S, Zachowski A, Ruelland E. The Arabidopsis DREB2 genetic pathway is constitutively repressed by basal phosphoinositide-dependent phospholipase C coupled to diacylglycerol kinase. Frontiers in Plant Science. 2013;4:307. DOI: 10.3389/fpls.2013.00307\n '},{id:"B39",body:'\n Zhai SM, Gao Q, Xue HW, Sui ZH, Yue GD, Yang AF, Zhang JR. Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance. Planta. 2012;235:69–84. DOI: 10.1007/s00425-011-1490-0\n '},{id:"B40",body:'\n Zhu J-K. Salt and Drought Stress Signal Transduction in Plants. Annual review of plant biology. 2002;53:247–273. DOI: 10.1146/annurev.arplant.53.091401.143329.\n '},{id:"B41",body:'\n Jacob T, Ritchie S, Assmann SM, Gilroy S. Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity. Proceedings of the National Academy of Sciences of the United States of America. 1999;96:12192–97. DOI: 10.1073/pnas.96.21.12192\n '},{id:"B42",body:'\n Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal. 2002;31:279–292. DOI: 10.1046/j.1365-313X.2002.01359.x\n '},{id:"B43",body:'\n Xu Y, Wei H, Juhua L, Jianbin Z, Caihong J, Hongxia M, Biyu X, Zhiqiang J. A banana aquaporin gene, MaPIP1;1, is involved in tolerance to drought and salt stresses. BMC Plant Biology. 2014;14:59. DOI: 10.1186/1471-2229-14-59\n '},{id:"B44",body:'\n Li H, Yao W, Fu Y, Li S, Guo Q. De Novo Assembly and discovery of genes that are involved in drought tolerance in Tibetan Sophora moorcroftiana. PLoS ONE 2015;10(1):e111054. DOI: 10.1371/journal.pone.0111054\n '},{id:"B45",body:'\n Li J, Ban L, Wen H, Wang Z, Dzyubenko N, Chapurin V, Gao H, Wang X. An aquaporin protein is associated with drought stress tolerance. Biochemical Biophysical Research Communication. 2015;459:208–213. DOI: 10.1016/j.bbrc.2015.02.052\n '},{id:"B46",body:'\n Osakabe Y, Arinaga N, Umezawa T, Katsura S, Nagamachi K, Tanaka H, Ohiraki H, Yamada K, Seo SU, Abo M Yoshimura E, Shinozaki K, Yamaguchi-Shinozaki K. Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis. The Plant Cell 2013;25:609–624. DOI: 10.1105/tpc.112.105700\n '},{id:"B47",body:'\n Vicente-Agullo F, Rigas S, Desbrosses G, Dolan L, Hatzopoulos P, Grabov A. Potassium carrier TRH1 is required for auxin transport in Arabidopsis roots. The Plant Journal. 2004;40:523–535. DOI: 10.1111/j.1365-313X.2004.02230.x\n '},{id:"B48",body:'\n Zhang C, Zhang L, Zhang S, Zhu S, Wu P, Chen Y, Li M, Jiang H, Wu G. Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress. BMC Plant Biology. 2015;15:17. DOI: 10.1371/journal.pone.0097878\n '},{id:"B49",body:'\n Wisniewski ME, Bassett CL, Renaut J, Farrell R, Tworkoski T, Artlip TS. Differential regulation of two dehydrin genes from peach (Prunus persica) by photoperiod, low temperature and water deficit. Tree Physiology. 2006;26:575–584. DOI: 10.1093/treephys/26.5.575\n '},{id:"B50",body:'\n Yoshimura K, Masuda A, Kuwano M, Yokota A, Akashi K. Programmed proteome response for drought avoidance/tolerance in the root of a C3 xerophyte (wild watermelon) under water deficits. Plant Cell Physiology. 2008;49:226–241. DOI: 10.1093/pcp/pcm180\n '},{id:"B51",body:'\n Degenhardt B, Gimmler H. Cell wall adaptations to multiple environmental stresses in maize roots. Journal of Experimental Botany. 2000;51:595–603. DOI: 10.1093/jexbot/51.344.595\n '},{id:"B52",body:'\n Hura T, Hura K, Dziurka K, Ostrowska A, Bączek-Kwinta R, Grzesiak M. An increase in the content of cell wall-bound phenolics correlates with the productivity of triticale under soil drought. Journal of Plant Physiology. 2012;169:1728–1736.61. DOI: 10.1016/j.jplph.2012.07.012\n '},{id:"B53",body:'\n Alam I, Sharmin SA, Kim KH, Yang JK, Choi MS, Lee BH. Proteome analysis of soybean roots subjected to short-term drought stress. Plant and Soil. 2010;333:491–505. DOI: 10.1007/s11104-010-0365-7\n '},{id:"B54",body:'\n Mohammadi PP, Moieni A, Komatsu S. Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress. Amino Acids. 2012;43:2137–2152. DOI: 10.1007/s00726-012-1299-6\n '},{id:"B55",body:'\n Hu H, Xiong L. Genetic engineering and breeding of drought-resistant crops. Annual Reviews of Plant Biology. 2014;65:715–741. DOI: 10.1146/annurev-arplant-050213-040000\n '},{id:"B56",body:'\n Asthir B. Protective mechanisms of heat tolerance in crop plants. Journal of Plant Interactions. 2015;10:202–210. DOI: 10.1080/17429145.2015.1067726\n '},{id:"B57",body:'\n Hasanuzzaman M, Nahar K, Alam MdM, Roychowdhury, Rajib, and Fujita M. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Science. 2013;14(5):9643–9684. DOI: 10.3390/ijms14059643\n '},{id:"B58",body:'\n Saidi Y, Peter M, Finka A, Cicekli C, Vigh L, Goloubinoff P. Membrane lipid composition affects plant heat sensing and modulates Ca2+-dependent heat shock response. Plant Signaling and Behavior. 2010;5:1530–1533. DOI: 10.4161/psb.5.12.13163\n '},{id:"B59",body:'\n Li Z, Yue HY, Xing D. MAP Kinase 6-mediated activation of vacuolar processing enzyme modulates heat shock-induced programmed cell death in Arabidopsis. New Phytologist. 2012;195:85–96. DOI: 10.3389/fpls.2015.00234\n '},{id:"B60",body:'\n Suzuki N, Sejima H, Tam R, Schlauch K, Mittler R. Identification of the MBF1 heat-response regulon of Arabidopsis thaliana. The Plant Journal. 2011;66:844–851. DOI: 10.1111/j.1365-313X.2011.04550.x\n '},{id:"B61",body:'\n Miller G, Schlauch K, Tam R, Cortes D, Torres MA, Shulaev V, Dangl JL, Mittler R. The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli. Science Signaling. 2009;2. DOI: 10.1126/scisignal.2000448\n '},{id:"B62",body:'\n Liu HT, Gao F, Li GL, Han JL, Liu DL, Sun DY, Zhau RG. The calmodulin-binding protein kinase 3 is part of heat-shock signal transduction in Arabidopsis thaliana. The Plant Journal. 2008;55:760–773. DOI: 10.1111/j.1365-313X.2008.03544.x\n '},{id:"B63",body:'\n Baniwal SK, Bharti K, Chan KY, Fauth M, Ganguli A, Kotak S, Mishra SK, Nover L, Port M, Scharf KD, Tripp J, Weber C, Zielinski D, von Koskull-Döring P. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. Journal of Biosciences. 2004;29:471–487.\n '},{id:"B64",body:'\n Suzuki N, Bajad S, Shuman J, Shulaev V, Mittler R. The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana. The Journal of Biological Chemistry. 2008, 283:9269–9275. DOI: 10.1074/jbc.M709187200\n '},{id:"B65",body:'\n Khan MIR, Iqbal N, Masood A, Per TS, Khan NA. Salicylic acid alleviates adverse effects of heat stress on photosynthesis through changes in proline production and ethylene formation. Plant Signaling & Behavior. 2013; 8(11):e26374. DOI: 10.4161/psb.26374.\n '},{id:"B66",body:'\n Meiri D, Breiman A. Arabidopsis ROF1 (FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting the accumulation of HsfA2-regulated sHSPs. The Plant Journal 2009;59:387–399. DOI: 10.1111/j.1365-313X.2009.03878.x\n '},{id:"B67",body:'\n Bokszczanin KL, Fragkostefanakis S. Perspectives on deciphering mechanisms underlying plant heat stress response and thermotolerance. Frontiers in Plant Science. 2013;4:315– 335. DOI: 10.3389/fpls.2013.00315\n '},{id:"B68",body:'\n Sidrauski C, Walter P. The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response. Cell 1997;90:1031–1039. DOI: 10.1016/S0092-8674(00)80369-4\n '},{id:"B69",body:'\n Deng Y, Humbert S, Liu JX, Srivastava R, Rothstein SJ, Howell SH. Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2011;108:7247–7252. DOI: 10.1073/pnas.1102117108\n '},{id:"B70",body:'\n Sugio A, Dreos R, Aparicio F, Maule AJ. The cytosolic protein response as a subcomponent of the wider heat shock response in Arabidopsis. The Plant Cell 2009;21:642–654. DOI: 10.1105/tpc.108.062596\n '},{id:"B71",body:'\n Liu J, Sun N, Liu M, Liu J, Du B, Wang X, Qi X. An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing. Plant Physiology. 2013;162:512–521. DOI: 10.1104/pp.112.205864\n '},{id:"B72",body:'\n Sato H, Mizoi J, Tanaka H, Maruyama K, Qin F, Osakabe Y, Morimoto K. Ohori T, Kusakabe K, Nagata M, Shinozaki K, Yamaguchi-Shinozakia K. Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits. The Plant Cell 2014;26:4954–4973. DOI: 10.1105/tpc.114.132928\n '},{id:"B73",body:'\n Gao HB, Brandizzi F, Benning C, Larkin RM. A membrane-tethered transcription factor defines a branch of the heat stress response in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America. 2008;105:16398–16403. DOI: 10.1073/pnas.0808463105\n '},{id:"B74",body:'\n Li SJ, Fu QT, Huang WD, Yu DQ. Functional analysis of an Arabidopsis transcription factor WRKY25 in heat stress. Plant Cell Reports. 2009;28:683–693. DOI: 10.1007/s00299-008-0666-y\n '},{id:"B75",body:'\n Wu XL, Shiroto Y, Kishitani S, Ito Y, Toriyama K. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter. Plant Cell Reports. 2009;28:21–30. DOI: 10.1007/s00299-008-0614-x\n '},{id:"B76",body:'\n Proveniers MCG, van Zanten M. High temperature acclimation through PIF4 signaling. Trends in Plant Science. 2013;18:59–64. DOI: 10.1016/j.tplants.2012.09.002\n '},{id:"B77",body:'\n Ma D, Li X, Guo X, Chu J, Fang S, YanC, Noel JP, Liu H. Cryptochrome 1 interacts with PIF4 to regulate high temperature-mediated hypocotyl elongation in response to blue light. Proceedings of the National Academy of Sciences of the United States of America. 2015;113:224–229. DOI: 10.1073/pnas.1511437113\n '},{id:"B78",body:'\n Erkina TY, Zou Y, Freeling S, Vorobyev VI, Erkine AM. Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes. Nucleic Acids Research. 2010;38:1441–1449. DOI: 10.1093/nar/gkp1130\n '},{id:"B79",body:'\n Rasheed R, Wahid A, Farooq M, Basra SMA. Role of proline and glycinebetaine in improving heat tolerance in sprouting sugarcane (Saccharum sp.) buds. Plant Growth Regulation.2011;65:35–45. DOI: 10.1007/s10725-011-9572-3\n '},{id:"B80",body:'\n Kapoor D, Sharma R, Handa N, Kaur H, Rattan A, Yadav P, Gautam V, Kaur R, Bhardwaj R. Redox homeostasis in plants under abiotic stress. Role of electron carriers, energy metabolism mediators and proteinaceous thiols. Frontiers in Environmental Science. 2015;3:13. DOI: 10.3389/fenvs.2015.00013\n '},{id:"B81",body:'\n Priest HD, Fox SE, Rowley ER, Murray JR, Michael TP, Mockler TC. Analysis of global gene expression in Brachypodium distachyon reveals extensive network plasticity in response to abiotic stress. PLoS ONE. 2014;9:e87499. DOI: 10.1371/journal.pone.0087499\n '},{id:"B82",body:'\n Zhang Y, Xu L, Zhu X, Gong Y, Xiang F, Sun X, Liu L. Proteomic analysis of heat stress response in leaves of radish (Raphanus sativus L.). Plant Molecular Biology Reports. 2013, 31:195–203. DOI: 10.1007/s11105-012-0486-7\n '},{id:"B83",body:'\n Chen X, Zhang W, Zhang B, Zhou J, Wang Y, Yang Q, Ke Y, He H. Phosphoproteins regulated by heat stress in rice leaves. Proteome Science. 2011;9: 37. DOI: 10.1186/1477-5956-9-37\n '},{id:"B84",body:'\n Li W, Wei Z, Qiao Z, Wu Z, Cheng L, Wang Y. Proteomics analysis of alfalfa response to heat stress. PLoS ONE. 2013a;8:e82725. DOI: 10.1371/journal.pone.0082725\n '},{id:"B85",body:'\n Du HM, Wang ZL, Yu WJ, Liu YM, Huang BR. Differential metabolic responses of perennial grass Cynodon transvaalensis × Cynodon dactylon (C4) and Poa pratensis (C3) to heat stress. Physiologia Plantarum. 2011;141:51–264. DOI: 10.1111/j.1399-3054.2010.01432.x\n '},{id:"B86",body:'\n Yu J, Du H, Xu M, Huang B. Metabolic responses to heat stress under elevated atmospheric CO2 concentration in a cool-season grass species. Journal of the American Society for Horticultural Science. 2012b;137:221–228. DOI: 10.1111/j.1399-3054.2010.01432.x\n '},{id:"B87",body:'\n Goswami S, Kumar RR, Rai RD. Heat-responsive microRNAs regulate the transcription factors and heat shock proteins in modulating thermostability of starch biosynthesis enzymes in wheat (Triticum aestivum L.) under the heat stress. Australian Journal of Crop Science. 2014;8:697—705.\n '},{id:"B88",body:'\n Hozain M, Abdelmageed H, Lee J, Kang M, Fokar M, Allen RD, Holaday AS. Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress. Journal of Plant Physiology. 2012;169:1261—1270. DOI: 10.1016/j.jplph.2012.04.007\n '},{id:"B89",body:'\n Sasaki K, Saito T, Lämsä M, Oksman-Caldentey KM, Suzuki M, Ohyama K, Muranaka T, Ohara K, Yazaki K. Plants utilize isoprene emission as a thermo tolerance mechanism. Plant Cell Physiology. 2007;48:1254—126. DOI: 10.1093/pcp/pcm104\n '},{id:"B90",body:'\n Macková H, Hronková M, Dobrá J, Turecková V, Novák O, Lubovská Z, Motyka V, Haisel D, Hájek Prásil ITT, Gaudinová A, Storchová H, Ge E, Werner T, Schmülling T, Vanková R. Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expression. Journal of Experimental Botany. 2013;64:2805—2815. DOI: 10.1093/jxb/ert131\n '},{id:"B91",body:'\n Feng L, Wang KJ, Li Y, Tan Y, Kong J, H L, Y L, Zhu Y. Over expression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants. Plant Cell Reports. 2007;26:1635—1646. DOI: 10.1007/s00299-006-0299-y\n '},{id:"B92",body:'\n Chinnusamy V, Zhu J, Zhu JK. Cold stress regulation of gene expression in plants. Trends Plant Science. 2007;12:444–451. DOI: 10.1016/j.tplants.2007.07.002\n '},{id:"B93",body:'\n Sanghera GS, Wani SH, Hussain W, Singh NB. Engineering cold stress tolerance in crop plants. Current Genomics. 2011;12:30–43. DOI: 10.2174/138920211794520178\n '},{id:"B94",body:'\n Ye H, Du H, Tang N, Li X, Xiong L. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Molecular Biology. 2009;71:291–305. DOI: 10.1007/s11103-009-9524-8\n '},{id:"B95",body:'\n Miura K, and Furumoto T. Cold signaling and cold response in plants. International Journal of Molecular Science. 2013;14(3):5312‐5337. DOI: 10.3390/ijms14035312\n '},{id:"B96",body:'\n Knight MR, Campbell AK, Smith SM, Trewavas AJ. Transgenic plant aequorin reports the effects of touch and cold‐shock and elicitors on cytoplasmic calcium. Nature. 1991;352(6335):524–6. DOI: 10.1038/352524a0\n '},{id:"B97",body:'\n Furuya T, Matsuoka D, Nanmori T. Membrane rigidification functions upstream of the MEKK1-MKK2-MPK4 cascade during cold acclimation in Arabidopsis thaliana. FEBS Letters. 2014;588:2025–2030. DOI: 10.1016/j.febslet.2014.04.032\n '},{id:"B98",body:'\n Francia E, Rizza F, Cattivelli L, Stanca AM, Galiba G, Toth B, Hayes PM, Skinner JS, Pecchioni N. Two loci on chromosome 5H determine low-temperature tolerance in a ‘Nure’ (winter) × ‘Tremois’ (spring) barley map. Theoretical Applied Genetics. 2004;108:670—680. DOI: 10.1007/s00122-003-1468-9\n '},{id:"B99",body:'\n Huang C, Ding S, Zhang H, Du H, An L. CIPK7 is involved in cold response by interacting with CBL1 in Arabidopsis thaliana. Plant Science. 2011;181:57–64. DOI: 10.1016/j.plantsci.2011.03.011\n '},{id:"B100",body:'\n Doherty CJ, Buskirk HAV, Myers SJ, Thomashow MF. Roles for Arabidopsis CAMTA transcription factors in cold-regulated gene expression and freezing tolerance. The Plant Cell. 2009;21:972–984. DOI: 10.1105/tpc.108.063958\n '},{id:"B101",body:'\n Chinnusamy V, Zhu J-K, Sunkar R. Gene Regulation during cold stress acclimation in plants. Methods in molecular biology. 2010;639:39–55. DOI: 10.1007/978-1-60761-702-0_3.\n '},{id:"B102",body:'\n Takahashi S, Murata N. How do environmental stresses accelerate photo-inhibition? Trends in Plant Science. 2008;13:178–182. DOI: 10.1016/j.tplants.2008.01.005\n '},{id:"B103",body:'\n Teige M, Scheikl E, Eulgem T, Doczi R, Ichimura K, Shinozaki K, Dangl JL, Hirt H. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis. Molecular Cell. 2004;15:141–152. DOI: 10.1016/j.molcel.2004.06.023\n '},{id:"B104",body:'\n Tarkowski ŁP, Van den Ende W. Cold tolerance triggered by soluble sugars: a multifaceted countermeasure. Frontiers in Plant Science. 2015;6. DOI: 10.3389/fpls.2015.00203\n '},{id:"B105",body:'\n Chinnusamy V, Ohta M, Kanrar S, Lee B H, Hong X, Agarwal M, Zhu JK. ICE1. A regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes and Development. 2003;17:1043–1054. DOI: 10.1101/gad.1077503\n '},{id:"B106",body:'\n Liu L, Duan L, Zhang J, Zhang Z, Mi G, Ren H. Cucumber (Cucumis sativus L.) over-expressing cold-induced transcriptome regulator ICE1 exhibits changed morphological characters and enhances chilling tolerance. Scientia Horticulturae. 2010;124:29–33. DOI: 10.1016/j.scienta.2009.11.018\n '},{id:"B107",body:'\n Medina J, Catala R, Salinas J. The CBFs: three Arabidopsis transcription factors to cold acclimate. Plant Science. 2011;180:3–11. DOI: 10.1016/j.plantsci.2010.06.019\n '},{id:"B108",body:'\n Zeller G, Henz SR, Widmer CK, Sachsenberg T, Ratsch G, Weigel D, Laubinger S. Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays. The Plant Journal. 2009;58:1068–1082. DOI: 10.1111/j.1365-313X.2009.03835.x\n '},{id:"B109",body:'\n Novillo F, Alonso JM, Ecker JR, Salinas J. CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2004;101:3985–3990. DOI: 10.1073/pnas.0303029101\n '},{id:"B110",body:'\n Novillo F, Medina J, Salinas J. Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proceedings of the National Academy of Sciences of the United States of America. 2007;104:21002–21007. DOI: 10.1073/pnas.0705639105\n '},{id:"B111",body:'\n Krasensky J, Jonak C. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany. 2012;63:1593–1608. DOI: 10.1093/jxb/err460\n '},{id:"B112",body:'\n Lee DG, Ahsan N, Lee SH, Lee JJ, Bahk JD, Kang KY, Lee BH. Chilling stress-induced proteomic changes in rice roots. Journal of Plant Physiology. 2009;166:1–11. DOI: 10.1016/j.jplph.2008.02.001\n '},{id:"B113",body:'\n FAO. Land and Plant Nutrition Management Service [Internet]. 2008. Available from: http://wwwfaoorg/ag/agl/agll/spush [Accessed: 2016-05-03]\n '},{id:"B114",body:'\n Munns R, Tester M. Mechanisms of salinity tolerance. Annual Reviews in Plant Biology. 2008;59:651–681. DOI: 10.1146/annurev.arplant.59.032607.092911\n '},{id:"B115",body:'\n Shahbaz M, Ashraf M. Improving salinity tolerance in cereals. Critical Reviews in Plant Science. 2013;32:237–249. DOI: 10.1080/07352689.2013.758544\n '},{id:"B116",body:'\n Volkov V. Salinity tolerance in plants. Quantitative approach to ion transport starting from halophytes and stepping to genetic and protein engineering for manipulating ion fluxes Frontiers in Plant Science. 2015;6:873. DOI: 10.3389/fpls.2015.00873\n '},{id:"B117",body:'\n Hasegawa PM, Bressan RA, Zhu JK and HJ Bohnert. Plant cellular and molecular responses to high salinity. Annual Reviews in Plant Biology. 2000;51:463–499. DOI: 10.1146/annurev.arplant.51.1.463\n '},{id:"B118",body:'\n Jung JKH, McCouch S. Getting to the roots of it: genetic and hormonal control of root architecture. Frontiers in Plant Science. 2013;4:186. DOI: 10.3389/fpls.2013.00186\n '},{id:"B119",body:'\n Wang H, Wu Z, Chen Y, Yang C, Shi D. Effects of salt and alkali stresses on growth and ion balance in rice (Oryza sativa L.). Plant, Soil and Environment. 2011;57:286–294.\n '},{id:"B120",body:'\n Deinlein U, Stephen AB, Horie T, Luo W, Xu G, Schroeder JI. Plant salt tolerance mechanisms. Trends in Plant Science. 2014;19:371–379. DOI: 10.1016/j.tplants.2014.02.001\n '},{id:"B121",body:'\n Pandey N, Ranjan A, Pant P, Tripathi RK, Ateek F, Pandey HP, Patre UV, Sawant SV. CAMTA 1 regulates drought responses in Arabidopsis thaliana. BMC Genomics. 2013;14:216. DOI: 10.1186/1471-2164-14-216\n '},{id:"B122",body:'\n Weng H, Yoo CY, Gosney MJ, Hasegawa PM, Mickelbart MV. Poplar GTL1 is a Ca2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance. PLoS ONE. 2012;7:e32925. DOI: 10.1371/journal.pone.0032925\n '},{id:"B123",body:'\n Yoo JH, Park CY, Kim JC, Heo WD, Cheong MS, Park HC, Kim MC, Moon BC, Choi MS, Kang YH, Lee JH, Kim HS, Lee SM, Yoon HW, Lim CO, Yun DJ, Lee SY, Chung WS, Cho MJ. Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis. Journal of Biological Chemistry. 2005;280:3697–3706. DOI: 10.1074/jbc.M408237200\n '},{id:"B124",body:'\n Liu C, Mao B, Ou S, Wang W, Liu L, Wu Y, Chu C, Wang X. OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice. Plant Molecular Biology. 2014;84:19–36. DOI: 10.1007/s11103-013-0115-3\n '},{id:"B125",body:'\n Jiang Y, Deyholos MK. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses. Plant Molecular Biology. 2009;69:91–105. DOI: 10.1007/s11103-008-9408-3\n '},{id:"B126",body:'\n Yamaguchi-Shinozaki K, Shinozaki K. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. In: Novartis Foundation Symposium 236—Rice Biotechnology: Improving Yield, Stress Tolerance and Grain Quality; February 2001; discussion 186-9; 236:176–186; DOI: 10.1002/9780470515778.ch13\n '},{id:"B127",body:'\n Cui MH, Yoo KS, Hyoung S, Nguyen HT, Kim YY, Kim HJ, Ok SH, Yoo SD, Shin JS. An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance. FEBS Letters. 2013;587:1773–1778. DOI: 10.1016/j.febslet.2013.04.028\n '},{id:"B128",body:'\n Jiang Y, Yang B, Deyholos MK. Functional characterization of the Arabidopsis bHLH92 transcription factor in abiotic stress. Molecular Genetics and Genomics 2009;282:503–516. DOI: 10.1007/s00438-009-0481-3\n '},{id:"B129",body:'\n Chen Y, Zong J, Tan Z, Li L, Hu B, Chen C, Chen J, Liu J. Systematic mining of salinity-tolerant genes in Halophyte-Zoysia matrella through cDNA expression library screening. Plant Physiology and Biochemistry 2015b;89:44–52. DOI: 10.1016/j.plaphy.2015.02.007\n '},{id:"B130",body:'\n Hur J, Jung KH, Lee CH, An G. Stress-inducible OsP5CS2 gene is essential for salt and cold tolerance in rice. Plant Science 2004;167:417–426. DOI: 10.1016/j.plantsci.2004.04.009\n '},{id:"B131",body:'\n Ismail A, Takeda S, and Nick P. Life and death under salt stress: same players, different timing? Journal of Experimental Botany.2014;65:2963–2979. DOI: 10.1093/jxb/eru159\n '},{id:"B132",body:'\n Quintero FJ, Martinez-Atienza J, Villalta I, Jiang X, Kim WY, Ali Z, Fujii H, Mendoza I, Yun DJ, Zhu JK, and Pardo JM. Activation of the plasma membrane Na/H antiporter Salt-Overly Sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proceedings of the National Academy of Sciences of the United States of America. 2011;108:2611–2616. DOI: 10.1073/pnas.1018921108\n '},{id:"B133",body:'\n Du W, Lin H, Chen S, Wu Y, Zhang J, Fuglsang AT, Palmgren MG, Wu W, Guo Y. Phosphorylation of SOS3-like calcium-binding proteins by their interacting SOS2-like protein kinases is a common regulatory mechanism in Arabidopsis. Plant Physiology. 2011;156:2235–2243. DOI: 10.1104/pp.111.173377\n '},{id:"B134",body:'\n Bassil E, Blumwald E. The ins and outs of intracellular ion homeostasis: NHX-type Cation/H+ transporters. Current Opinion in Plant Biology. 2014, 22:1–6. DOI: 10.1016/j.pbi.2014.08.002\n '},{id:"B135",body:'\n Yamaguchi T, Aharon GS, Sottosanto JB, Blumwald E. Vacuolar Na+/H+ antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2+ and pH-dependent manner. Proceedings of the National Academy of Sciences of the United States of America. 2005;102:16107–16112. DOI: 10.1073/pnas.0504437102\n '},{id:"B136",body:'\n Guan R, Qu Y, Guo Y, Yu L, Liu Y, Jiang J, Chen J, Ren Y, Liu G, Tian L, Jin L, Liu Z, Hong H, Chang R, Gilliham M, Qiu L. Salinity tolerance in soybean is modulated by natural variation in GmSALT3. The Plant Journal. 2014;80:937–950. DOI: 10.1111/tpj.12695\n '},{id:"B137",body:'\n Kumar R, Mustafiz A, Sahoo KK, Sharma V, Samanta S, Sopory SK, Pareek A, Singla-Pareek SL. Functional screening of cDNA library from a salt tolerant rice genotype Pokkali identifies mannose-1-phosphate guanyl transferase gene (OsMPG1) as a key member of salinity stress response. Plant Molecular Biology. 2012;79:555–568. DOI: 10.1007/s11103-012-9928-8\n '},{id:"B138",body:'\n Obata T, Kitamoto HK, Nakamura A, Fukuda A, Tanaka Y. Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells. Plant Physiology. 2007;144:1978–1985. DOI: 10.1104/pp.107.101154\n '},{id:"B139",body:'\n Chen Y, Chen C, Tan Z, Liu J, Zhuang L, Yang Z, Huang B. Functional identification and characterization of genes cloned from halophyte Seashore paspalum conferring salinity and cadmium tolerance. Frontiers in Plant Science. 2016;7:102. DOI: 10.3389/fpls.2016.00102\n '},{id:"B140",body:'\n Munns R, Gilliham M. Salinity tolerance of crops – what is the cost? New Phytologist. 2015;208:668–673. DOI: 10.1111/nph.13519\n '},{id:"B141",body:'\n Byrt CS, Xu B, Krishnan M, Lightfoot DJ, Athman A, Jacobs AK, Watson-Haigh NS, Munns R, Tester M, Gilliham M. The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat. The Plant Journal. 2014;80:516–26. DOI: 10.1111/tpj.12651\n '},{id:"B142",body:'\n Munns R, James RA, Xu B, Athman A, Conn SJ, Jordans C, Byrt CS, Hare RA, Tyerman SD, Tester M, Plett D, Gilliham M. Wheat grain yield on saline soils is improved by an ancestral Na⁺ transporter gene. Nature Biotechnology. 2012;30:360–4. 10.1038/nbt.2120\n '},{id:"B143",body:'\n Eswaran N, Parameswaran S, Sathram B, Anantharaman B, Kumar GRK, Tangirala SJ. Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas. BMC Biotechnology. 2010;10:23. DOI: 10.3389/fpls.2015.00920\n '},{id:"B144",body:'\n Zhou L, Wang C, Liu R, Han Q, Vandeleur RK, Du J, Tyerman S, Shou H. Constitutive overexpression of soybean plasma membrane intrinsic protein GmPIP1;6 confers salt tolerance. BMC Plant Biology. 2014;14:188. DOI: 10.1186/1471-2229-14-181\n '},{id:"B145",body:'\n Sade N, Gebretsadik M, Seligmann R, Schwartz A, Wallach R, Moshelion M. The role of tobacco Aquaporin1 in improving water use efficiency, hydraulic conductivity, and yield production under salt stress. Plant Physiology. 2010;152(1):245–254. DOI: 10.1104/pp.109.145854\n '},{id:"B146",body:'\n Gao Z, He X, Zhao B, Zhou C, Liang Y, Ge R, Shen Y, Huang Z. Overexpressing a putative aquaporin gene from wheat, TaNIP, enhances salt tolerance in transgenic Arabidopsis. Plant &Cell Physiology. 2010;51(5):767–775. DOI: 10.1093/pcp/pcq036\n '},{id:"B147",body:'\n Hu W, Yuan Q, Wang Y, Cai R, Deng X, Wang J, Zhou S, Chen M, Chen L, Huang C, Ma Z, Yang G, He G. Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco. Plant & Cell Physiology 2012;53(12):2127–2141. DOI: 10.1093/pcp/pcs154\n '},{id:"B148",body:'\n Zhou QY, Wang L, Cai X, Wang D, Hua XJ, Qu LQ, Lin JX, Chen T. Net sodium fluxes change significantly at anatomically distinct root zones of rice (Oryza sativa L.) seedlings. Journal of Plant Physiology. 2011;168(11):1249–1255. DOI: 10.1016/j.jplph.2011.01.017\n '},{id:"B149",body:'\n Henderson SW, Baumann U, Blackmore DH, Walker AR, Walker RR, Gilliham M. Shoot chloride exclusion and salt tolerance in grapevine is associated with differential ion transporter expression in roots. BMC Plant Biology. 2014;14: 273. DOI: 10.1186/s12870-014-0273-8\n '},{id:"B150",body:'\n Vandeleur RK, Sullivan W, Athman A, Jordans C, Gilliham M, Kaiser BN, Tyerman SD. Rapid shoot-to-root signaling regulates root hydraulic conductance via aquaporins. Plant, Cell and Environment. 2014;37(2):520–538. DOI: 10.1111/pce.12175\n '},{id:"B151",body:'\n Xu C, Wang M, Zhou L, Quan T, Xia G. Heterologous expression of the wheat aquaporin gene TaTIP2;2 compromises the abiotic stress tolerance of Arabidopsis thaliana. PLoS ONE. 2013;8:e79618. DOI: 10.1371/journal.pone.0079618\n '},{id:"B152",body:'\n Sani E, Herzyk P, Perrella G, Colot V, Amtmann A. Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome. Genome Biology. 2013;14:R59. DOI: 10.1186/gb-2013-14-6-r59\n '},{id:"B153",body:'\n Wang X, Fan P, Song H, Chen X, Li X, Li Y. Comparative proteomic analysis of differentially expressed proteins in shoots of Salicornia europaea under different salinity. Journal of Proteome Research. 2009;8:3331–3345. DOI: 10.1021/pr801083a\n '},{id:"B154",body:'\n Zhou S, Sauvé RJ, Liu Z, Reddy S, Bhatti S, Hucko SD, Fish T, Thannhauser TW. Identification of salt-induced changes in leaf and root proteomes of the wild tomato, Solanum chilense. Journal of the American Society for Horticultural Science. 2011;136:288–302.\n '},{id:"B155",body:'\n Wang L, Liu X, Liang M, Tan F, Liang W, Chen Y, Lin Y, Huang L, Xing J, Chen W. Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress. PLoS ONE. 2014;9:e83141. DOI: 10.1371/journal.pone.0083141\n '},{id:"B156",body:'\n Cheng T, Chen J, Zhang J, Shi S, Zhou Y, Lu L, Wang P, Jiang Z, Yang J, Zhang S, Shi J. Physiological and proteomic analyses of leaves from the halophyte Tangut nitraria reveals diverse response pathways critical for high salinity tolerance. Front in Plant Science. 2015;6:30. DOI: 10.3389/fpls.2015.00030\n '},{id:"B157",body:'\n Nadeem SM, Ahmad M, Zahir ZA, Javaid A, Ashraf M. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances. 2014;32:429–448. DOI: 10.1016/j.biotechadv.2013.12.005\n '},{id:"B158",body:'\n Roy SJ, Huang W, Wang XJ, Evrard A, Schmoeckel SM, Zafar ZU, Tester M. A novel protein kinase involved in Na+ exclusion revealed from positional cloning. Plant, Cell and Environment. 2013;36:553–568. DOI: 10.1111/j.1365-3040.2012.02595.x\n '},{id:"B159",body:'\n Platten JD, Egdane JA, Ismail AM. Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima. Many sources, many genes, one mechanism? BMC Plant Biology. 2013;13:32. DOI: 10.1186/1471-2229-13-32\n '},{id:"B160",body:'\n Zhou S, Zhang Z, Tang Q, Lan H, Li Y, Luo P. Enhanced V-ATPase activity contributes to the improved salt tolerance of transgenic tobacco plants overexpressing vacuolar Na+/H+ antiporter AtNHX1. Biotechnology Letters 2011;33:375–380. DOI: 10.1007/s10529-010-0418-2\n '},{id:"B161",body:'\n Bailey-Serres J, Lee SC, Brinton E. Waterproofing crops: effective flooding survival strategies. Plant Physiology. 2012;160:1698 –1709. DOI: 10.1104/pp.112.208173\n '},{id:"B162",body:'\n Jackson MB, Ismail AM. Electrons, water and rice fields: plant response and adaptation to flooding and submergence stress. AoB Plants. 2015;7:plv078. DOI: 10.1093/aobpla/plv078\n '},{id:"B163",body:'\n Arshad M, and Frankenberger WTJ. Production and stability of ethylene in soil. Biology and Fertility of Soils. 1990;10:29-34. DOI: 10.1007/BF00336121\n '},{id:"B164",body:'\n Gibbs J, Greenway H. Mechanisms of anoxia tolerance in plants: growth, survival and anaerobic catabolism. Functional Plant Biology. 2003;30:1–47. DOI: 10.1071/PP98095\n '},{id:"B165",body:'\n Herzog M, Striker GG, Colmer TD, Pedersen O. Mechanisms of waterlogging tolerance in wheat—a review of root and shoot physiology. Plant, Cell and Environment. 2016;39(5):1068–1086. DOI: 10.1111/pce.12676\n '},{id:"B166",body:'\n Jung KH, Seo YS, Walia H, Cao P, Fukao T, Canlas P, Amonpant F, Bailey-Serres J, Ronald PC. The submergence tolerance regulator Sub1A mediates stress-responsive expression of AP2/ERF transcription factors. Plant Physiology. 2010;152:1674–1692. DOI: 10.1104/pp.109.152157\n '},{id:"B167",body:'\n Xu K, Mackill DJ. A major locus for submergence tolerance mapped on rice chromosome 9. Molecular Breeding. 1996;2:219–224. DOI: 10.1007/BF00564199\n '},{id:"B168",body:'\n Hattori Y, Nagai K, Furukawa S, Song XJ, Kawano R, Sakakibara H, Wu J, Matsumoto T, Yoshimura A, Kitano H, Matsuoka M, Mori H, Ashikari M. The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature. 2009;460:1026–1030. DOI: 10.1038/nature08258\n '},{id:"B169",body:'\n Nagai K, Kondo Y, Kitaoka T, Noda T, Kuroh T, Angeles-Shim RB, Yasui H, Yoshimura A, Ashikari M. QTL analysis of internode elongation in response to gibberellin in deep water rice. AoB Plants. 2014;6:plu028. DOI: 10.1093/aobpla/plu028\n '},{id:"B170",body:'\n Campbell MT, Proctor CA, Dou Y, Schmitz AJ, Phansak P, Kruger GR, Zhang C, Walia H. Genetic and molecular characterization of submergence response identifies subtol 6 as a major submergence tolerance locus in maize. PLoS ONE. 2015;10(3):e0120385.\n '},{id:"B171",body:'\n Huang S, Hill RD, Wally OSD, Dionisio G, Ayele BT, Jami SK, Stasolla C. Hemoglobin control of cell survival/death decision regulates in vitro plant embryogenesis. Plant Physiology. 2014;165:810–825. DOI: 10.11.04/pp.114.239335\n '},{id:"B172",body:'\n Jackson MB, Ram PC. Physiological and molecular basis of susceptibility and tolerance of rice plants to complete submergence. Annals of Botany. 2003;91:227–241. DOI: 10.1093/aob/mcf242\n '},{id:"B173",body:'\n Nishiuchi S, Yamauchi T, Takahashi H, Kotula L, Nakazono M. Mechanisms for coping with submergence and waterlogging in rice. Rice. 2012;5:2. DOI: 10.1186/1939-8433-5-2\n '},{id:"B174",body:'\n Voortman RL. Micronutrients in agriculture and the world food system – future scarcity and implications. Centre for World Food Studies (SOW-VU), VU University, Amsterdam 2012;\n '},{id:"B175",body:'\n Gui QZ, Bao LM, Chang ZR, Bao CL. Timing and level of nitrogen supply affect nitrogen distribution and recovery in two contrasting oat genotypes. Journal of Plant Nutrition and Soil Science. 2012;175:614–621. DOI: 10.1002/jpln.201100279\n '},{id:"B176",body:'\n Hermans C, Hammond JP, White PJ, Verbruggen N. How do plants respond to nutrient shortage by biomass allocation? Trends in Plant Science. 2006;11:610–617. DOI: 10.1016/j.tplants.2006.10.007\n '},{id:"B177",body:'\n Kant S, Bi YM, Rothstein SJ. Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. Journal of Experimental Botany. 2011a;62:1499–1509. DOI: 10.1093/jxb/erq297\n '},{id:"B178",body:'\n Nacry P, Bouguyon E, Gojon A. Nitrogen acquisition by roots: physiological and developmental mechanisms ensuring plant adaptation to a fluctuating resource. Plant and Soil.2013;370:1–29. DOI: 10.1007/s11104-013-1645-9\n '},{id:"B179",body:'\n Zhang H, Forde BG. An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture. Science. 1998;279:407–409. DOI:\n '},{id:"B180",body:'\n Kotur Z, Mackenzie N, Ramesh S, Tyerman SD, Kaiser BN, Glass AD. Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1. New Phytologist. 2012;194:724–731. DOI: 10.1111/j.1469-8137.2012.04094.x\n '},{id:"B181",body:'\n Sun J, Zheng N. Molecular mechanism underlying the plant NRT1.1 dual-affinity nitrate transporter. Frontiers in Physiology. 2015;6:386. DOI: 10.3389/fphys.2015.00386\n '},{id:"B182",body:'\n De Angeli A, Monachello D, Ephritikhine G, Frachisse JM, Thomine S, Gambale F, Barbier-Brygoo H. The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles. Nature. 2006;442:939–942. DOI: 10.1038/nature05013\n '},{id:"B183",body:'\n Geiger D, Maierhofer T, Al-Rasheid KA, Scherzer S, Mumm P, Liese A, Ache P, Wellmann C, Marten I, Grill E, Romeis T, Hedrich R. Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1. Science Signaling. 2011;4:ra32. DOI: 10.1126/scisignal.2001346\n '},{id:"B184",body:'\n Segonzac C, Boyer JC, Ipotesi E, Szponarski W, Tillard P, Touraine B, Sommerer N, Rossignol M, Gibrat R. Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter. The Plant Cell. 2007;19:3760–3777. DOI: 10.1105/tpc.106.048173\n '},{id:"B185",body:'\n Li JY, Fu YL, Pike SM, Bao J, Tian W, Zhang Y, Chen CZ, Zhang Y, Li HM, Huang J, Li LG, Schroeder JI, Gassmann W, Gong JM. The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. The Plant Cell 2010;22:1633–1646. DOI: 10.1105/tpc.110.075242\n '},{id:"B186",body:'\n Wang YY, Tsay YF. Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport. The Plant Cell 2011;23:1945–1957. DOI: 10.1105/tpc.111.083618\n '},{id:"B187",body:'\n Wang YY, Hsu PK, Tsay YF. Uptake, allocation and signaling of nitrate. Trends in Plant Science. 2012;17:458–467. DOI: 10.1016/j.tplants.2012.04.006\n '},{id:"B188",body:'\n Hu H, Wang Y, Tsay Y. AtCIPK8, a CBL-interacting protein kinase, regulates the low-affinity phase of the primary nitrate response. The Plant Journal. 2009;57:264–278. DOI: 10.1111/j.1365-313X.2008.03685.x\n '},{id:"B189",body:'\n Jonassen EM, Sévin DC, Lillo C. The bZIP transcription factors HY5 and HYH are positive regulators of the main nitrate reductase gene in Arabidopsis leaves, NIA2, but negative regulators of the nitrate uptake gene NRT1.1. Journal of Plant Physiology. 2009;166:2071–2076. DOI: 10.1016/j.jplph.2009.05.010\n '},{id:"B190",body:'\n Konishi M, Yanagisawa S. Arabidopsis Nin-like transcription factors play a central role in nitrate signaling. Nature Communications. 2013;4:1503. DOI: 10.1038/ncomms2621\n '},{id:"B191",body:'\n Pinton R, Tomasi N, Zanin L. Molecular and physiological interactions of urea and nitrate uptake in plants. Plant Signaling & Behavior. 2015;11:e1076603. DOI: 10.1080/15592324.2015.1076603\n '},{id:"B192",body:'\n Yoneyama K, Xie X, Kusumoto D. Nitrogen deficiency as well as phosphorus deficiency in Sorghum promotes the production and exudation of 5-deoxystrigol, the host recognition signal for arbuscular. Planta. 2007b;227:125–132. DOI: 10.1007/s00425-007-0600-5\n '},{id:"B193",body:'\n Walch-Liu P, Ivanov II, Filleur S, Gan Y, Remans T, Forde BG. Nitrogen regulation of root branching. Annals of Botany. 2006;97:875–881. DOI: 10.1093/aob/mcj601\n '},{id:"B194",body:'\n Li J, Zhu S, Song X, Shen Y, Chen H, Yu J, Yi K, Liu Y, Karplus VJ, Wu P, Deng XW. A rice glutamate receptor-like gene is critical for the division and survival of individual cells in the root apical meristem. The Plant Cell 2006;18 340–349. DOI: 10.1105/tpc.105.037713\n '},{id:"B195",body:'\n Fuentes SI, Allen DJ, Ortiz-Lopez A, Hernandez G. Over-expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations. Journal of Experimental Botany. 2001;52:1071–1081. DOI: 10.1093/jexbot/52.358.1071\n '},{id:"B196",body:'\n Reddy MM, Ulaganathan K. Nitrogen nutrition, its regulation and biotechnological approaches to improve crop productivity. American Journal of Plant Sciences.2015;6:2745–2798. DOI: 10.4236/ajps.2015.618275\n '},{id:"B197",body:'\n Vance CP, Uhde-Stone C, Allan DL. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytologist. 2003;157:423–447. DOI:\n '},{id:"B198",body:'\n Song L, Liu D. Ethylene and plant responses to phosphate deficiency. Frontiers in Plant Science. 2015;6:796. DOI: 10.3389/fpls.2015.00796.\n '},{id:"B199",body:'\n Thibaud MC, Arrighi JF, Bayle V, Chiarenza S, Creff A, Bustos R, Paz-Ares J, Poirier Y, Nussaume L. Dissection of local and systemic transcriptional responses to phosphate starvation in Arabidopsis. The Plant Journal. 2010;64:775–789. DOI: 10.1111/j.1365-313X.2010.04375.x\n '},{id:"B200",body:'\n Wang Z, Rahman AB, Wang G, Ludewig U, Shen J, Neumann G. Hormonal interactions during cluster-root development in phosphate-deficient white lupin (Lupinus albus L.). J Plant Physiology. 2015;177:74–82. DOI: 10.1016/j.jplph.2014.10.022\n '},{id:"B201",body:'\n He CJ, Morgan PW, Drew MC. Enhanced sensitivity to ethylene in nitrogen or phosphate-starved roots of Zea mays L. during aerenchyma formation. Plant Physiology. 1992;98:137–142. DOI: 10.1104/pp.98.1.137\n '},{id:"B202",body:'\n Chacón-López A, Ibarra-Laclette E, Sánchez-Calderón L, Gutiérrez-Alanís D, Herrera-Estrella L. Global expression pattern comparison between low phosphorus insensitive 4 and WT Arabidopsis reveals an important role of reactive oxygen species and jasmonic acid in the root tip response to phosphate starvation. Plant Signaling & Behavior. 2011;6:382–392. DOI: 10.4161/psb.6.3.14160\n '},{id:"B203",body:'\n Lei M, Zhu C, Liu Y, Karthikeyan AS, Bressan RA, Raghothama KG, Liu D. Ethylene signaling is involved in regulation of phosphate starvation-induced gene expression and production of acid phosphatases and anthocyanin in Arabidopsis. New Phytologist. 2011b;189:1084–1095. DOI: 10.1111/j.1469-8137.2010.03555.x\n '},{id:"B204",body:'\n Song G, Liu S. Phosphorus speciation and distribution in surface sediments of the Yellow Sea and East China Sea and potential impacts on ecosystem. Acta Oceanologica Sinica. 2015;34:84–91. DOI: 10.1007/s13131-015-0653-4\n '},{id:"B205",body:'\n Yu H, Luo N, Sun L, Liu D. HPS4/SABRE regulates plant responses to phosphate starvation through antagonistic interaction with ethylene signaling. Journal of Experimental Botany 2012;63:4527–4538. DOI: 10.1093/jxb/ers131\n '},{id:"B206",body:'\n Wang L, Dong J, Gao Z, Liu D. The Arabidopsis gene HYPERSENSITIVE TO PHOSPHATE STARVATION3 encodes ETHYLENE OVERPRODUCTION1. Plant Cell Physiology 2012;53:1093–1105. DOI: 10.1093/pcp/pcs072\n '},{id:"B207",body:'\n Christians MJ, Gingerich DJ, Hansen M, Binder BM, Kieber JJ, Vierstra RD. The BTB ubiquitin ligases ETO1. EOL1 and EOL2 act collectively to regulate ethylene biosynthesis in Arabidopsis by controlling type-2 ACC synthase levels. The Plant Journal. 2009;57:332–345. DOI: 10.1111/j.1365-313X.2008.03693.x\n '},{id:"B208",body:'\n Wang KL, Yoshida H, Lurin C, Ecker JR. Regulation of ethylene gas biosynthesis by the ArabidopsisETO1 protein. Nature. 2004;428:945–950. DOI: 10.1038/nature02516\n '},{id:"B209",body:'\n Zhang Y, Wang X, Lu S, Liu D. A major root-associated acid phosphatase in Arabidopsis, AtPAP10, is regulated by both local and systemic signals under phosphate starvation. Journal of Experimental Botany. 2014;65(22):6577–6588. DOI: 10.1093/jxb/eru377.\n '},{id:"B210",body:'\n Li YS, Gao Y, Tian QY, Shi FL, Li LH, Zhang WH. Stimulation of root acid phosphatase by phosphorus deficiency is regulated by ethylene in Medicago falcata. Environmental and Experimental Botany. 2011;71:114–120. DOI: 10.1016/j.envexpbot.2010.11.007\n '},{id:"B211",body:'\n Nacry P, Canivenc G, Muller B, Azmi A, Van Onckelen H, Rossignol M. A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis. Plant Physiology. 2005;138:2061–2074. DOI: 10.1104/pp.105.060061.\n '},{id:"B212",body:'\n Karthikeyan AS, Varadarajan DK, Jain A, Held MA, Carpita NC, Raghothama KG. Phosphate starvation responses are mediated by sugar signaling in Arabidopsis. Planta. 2007;225:907–918. DOI: 10.1007/s00425-006-0408-8\n '},{id:"B213",body:'\n Niu YF, Chai RS, Jin GL, Wang H, Tang CX, Zhang YS. Responses of root architecture development to low phosphorus availability: a review. Annals of Botany. 2013;112(2):391–408. DOI: 10.1093/aob/mcs285.\n '},{id:"B214",body:'\n Karthikeyan AS, Varadarajan DK, Jain A, Held MA, Carpita NC, Raghothama KG. Phosphate starvation responses are mediated by sugar signaling in Arabidopsis. Planta. 2007;225(4):907–918. DOI: 10.1007/s00425-006-0408-8\n '},{id:"B215",body:'\n López-Bucio J, Hernandez-Abreu E, Sanchez-Calderon L, Nieto-Jacobo MF, Simpson J, Herrera-Estrella L. Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiology. 2002;129:244–256. DOI: 10.1104/pp.010934\n '},{id:"B216",body:'\n Jiang C, Gao X, Liao L, Harberd NP, Fu X. Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiology. 2007;145:1460–1470. DOI: 10.1104/pp.107.103788\n '},{id:"B217",body:'\n iura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan AS, Raghothama KG, Baek D, Koo YD, Jin JB, Bressan RA, Yun DJ, Hasegawa PM. The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proceedings of the National Academy of Sciences of the United States of America. 2005;102:7760–7765. DOI: 10.1073/pnas.0500778102\n '},{id:"B218",body:'\n Niu YF, Chai RS, Jin GL, Wang H, Tang CX, Zhang YS. Responses of root architecture development to low phosphorus availability: a review. Annals of Botany. 2013;112:391–408. DOI: 10.1093/aob/mcs285\n '},{id:"B219",body:'\n Ticconi CA, Lucero RD, Sakhonwasee S, Adamson AW, Creff A, Nussaume L, Desnos T, Abel S. ER-resident proteins PDR2 and LPR1 mediate the developmental response of root meristems to phosphate availability. Proceedings of the National Academy of Sciences of the United States of America. 2009;106:14174–14179. DOI: 10.1073/pnas.0901778106\n '},{id:"B220",body:'\n Müller J, Toev T, Heisters M, Teller J, Moore KL, Hause G, Dinesh DC, Bürstenbinder K, Abel S. Iron-dependent callose deposition adjusts root meristem maintenance to phosphate availability. Developmental Cell. 2015;33:216–230. DOI: 10.1016/j.devcel.2015.02.007\n '},{id:"B221",body:'\n Gamuyao R, Chin JH, Pariasca-Tanaka J, Pesaresi P, Catausan S, Dalid C, Slamet-Loedin I, Tecson-Mendoza EM, Wissuwa M, Heuer S. The protein kinase Pstol1 from traditional rice confers tolerance of phosphorus deficiency. Nature. 2012;488:535–539. DOI: 10.1038/nature11346\n '},{id:"B222",body:'\n Pariasca-Tanaka J, Chian J, Drame´ K, Dalid C, Heuer S, Wissuwa m. A novel allele of the P-starvation tolerance gene OsPSTOL1 from African rice (Oryza glaberrima Steud) and its distribution in the genus Oryza. Theoretical Applied Genetics. 2014;127:1387–1398. DOI: 10.1007/s00122-014-2306-y\n '},{id:"B223",body:'\n Gao N, Yanhua S, Min J, Shen W, Shi W. Transgenic tomato overexpressing ath-miR399d has enhanced phosphorus accumulation through increased acid phosphatase and proton secretion as well as phosphate transporter. Plant and Soil. 2010;334: 23–136. DOI: 10.1007/s11104-009-0219-3\n '},{id:"B224",body:'\n Chiou T, Aung K, Lin S, Wu C, Chiang S, Su C. Regulation of phosphate homeostasis by microRNA in Arabidopsis. The Plant Cell. 2006;18:412–421. DOI: 10.1105/tpc.105.038943\n '},{id:"B225",body:'\n López-Bucio J, Hernandez-Abreu E, Sanchez-Calderon L, Nieto-Jacobo MF, Simpson J, Herrera-Estrella L. Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiology. 2002;129:244–256. DOI: 10.1104/pp.010934\n '},{id:"B226",body:'\n Jiang C, Gao X, Liao L, Harberd NP, Fu X. Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiology. 2007;145:1460–1470. DOI: 10.1104/pp.107.103788\n '},{id:"B227",body:'\n Lopez-Raez JA, Charnikhova T, Gomez-Roldan V, Matusova R, Kohlen W, De Vos R, Verstappen F, Puech-Pages V, Bécard G, Mulder P, Bouwmeester H. Tomato strigolactones are derived from carotenoids and their biosynthesis is promoted by phosphate starvation. New Phytologist. 2008;178:863–874. DOI: 10.1111/j.1469-8137.2008.02406.x\n '},{id:"B228",body:'\n Umehara M, Hanada A, Magome H, Takeda-Kamiya N, Yamaguchi S. Contribution of strigolactones to the inhibition of tiller bud outgrowth under phosphate deficiency in rice. Plant and Cell Physiology. 2010;51:1118–1126. DOI: 10.1093/pcp/pcq084\n '},{id:"B229",body:'\n Kohlen W, Charnikhova T, Liu Q. Strigolactones are transported through the xylem and play a key role in shoot architectural response to phosphate deficiency in nonarbuscular mycorrhizal host. Plant Physiology. 2011;155:974–987. DOI: 10.1104/pp.110.164640\n '},{id:"B230",body:'\n Foo E, Yoneyama K, Hugill CJ, Quittenden LJ, Reid JB. Strigolactones and the regulation of pea symbioses in response to nitrate and phosphate deficiency. Molecular Plant. 2012;6:76–87. DOI: 10.1093/mp/sss115\n '},{id:"B231",body:'\n Mayzlish-Gati E, De-Cuyper C, Goormachtig S, Beeckman T, Vuylsteke M, Brewer PB, Beveridge CA, Yermiyahu U, Kaplan Y, Enzer Y, Wininger S, Resnick N, Cohen M, Kapulnik Y, Koltai H. Strigolactones are involved in root response to low phosphate conditions in Arabidopsis. Plant Physiology. 2012;160:1329–1341. DOI: 10.1104/pp.112.202358\n '},{id:"B232",body:'\n Liao H, Yan XL, Rubio G, Beebe SE, Blair MW, Lynch JP. Genetic mapping of basal root gravitropism and phosphorus acquisition efficiency in common bean. Functional Plant Biology. 2004;31:959–970. DOI: 10.1071/FP03255\n '},{id:"B233",body:'\n Beebe SE, Rojas-Pierce M, Yan XL Matthew W. Blair MM, Pedraza F, Muñoz F, Tohme J, Lynch JP. Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean. Crop Science. 2006;46: 413–423. DOI: 10.2135/cropsci2005.0226\n '},{id:"B234",body:'\n Ochoa IE, Blair MW, Lynch JP. QTL analysis of adventitious root formation in common bean under contrasting phosphorus availability. Crop Science 2006, 46:1609–1621. DOI: 10.2135/cropsci2005.12-0446\n '},{id:"B235",body:'\n Yan X, Liao H, Beebe SE, Blair MW, Lynch JP. QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean. Plant and Soil. 2004;265:17–29. DOI: 10.1007/s11104-005-0693-1\n '},{id:"B236",body:'\n Li YD, Wang YJ, Tong YP, Gao JG, Zhang JS, Chen SY. QTL mapping of phosphorus deficiency tolerance in soybean (Glycine max L. Merr.). Euphytica. 2005;142:137–142. DOI: 10.1007/s10681-005-1192-4\n '},{id:"B237",body:'\n Zhang D, Cheng H, Geng L Kan S, Cui S, Meng Q, Gai J, Yu, D. Detection of quantitative trait loci for phosphorus deficiency tolerance at soybean seedling stage. Euphytica. 2009;167:313–322. DOI: 10.1007/s10681-009-9880-0\n '},{id:"B238",body:'\n Hodson MJ. Metal toxicity and tolerance in plants [Internet]. 2012; Available from: http://www.hodsons.org/MartinHodson/Biochemist12.pdf [Accessed: 2016-05-03]\n '},{id:"B239",body:'\n Uexküll HR, Mutert E. Global extent, development and economic impact of acid soils. Plant and Soil. 1995;171:1–15. DOI: 10.1007/BF00009558\n '},{id:"B240",body:'\n Kochian LV. Cellular mechanisms of aluminum toxicity and resistance in plants. Annual Reviews in Plant Biology. 1995;46:237–260. DOI: 10.1146/annurev.pp.46.060195.001321\n '},{id:"B241",body:'\n Kinraide TB. Assessing the rhizotoxicity of the aluminate ion, Al(OH)4. Plant Physiology. 1990;93:1620–1625. DOI: 10.1146/annurev.pp.46.060195.001321\n '},{id:"B242",body:'\n Delhaize E, Ryan PR, Randall PJ. Aluminum tolerance in wheat (Triticum aestivum L.) (II. Aluminum-stimulated excretion of malic acid from root apices). Plant Physiology. 1993;103:695–702. DOI: 10.1104/pp.103.3.695\n '},{id:"B243",body:'\n Klug B, Horst WJ. Spatial characteristics of aluminum uptake and translocation in roots of buckwheat (Fagopyrum esculentum). Physiologia Plantarum. 2010;139:181–191. DOI: 10.1111/j.1399-3054.2010.01355.x\n '},{id:"B244",body:'\n Li JY, Liu J, Dong D, Jia X, McCouch SR, Kochian LV. Natural variation underlies alterations in Nramp aluminum transporter (NRAT1) expression and function that play a key role in rice aluminum tolerance. Proceedings of the National Academy of Sciences of the United States of America. 2014;111:6503–6508. DOI: 10.1073/pnas.1318975111\n '},{id:"B245",body:'\n Bian M, Zhou M, Sun D, Li C. Molecular approaches unravel the mechanism of acid soil tolerance in plants. The Crop Journal. 2013;1:91–104. DOI: 10.1016/j.cj.2013.08.002\n '},{id:"B246",body:'\n Zhou GF, Delhaize E, Zhou MX, Ryan PR. The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley. Annals of Botany. 2013;112:603–612. DOI: 10.1093/aob/mct135\n '},{id:"B247",body:'\n Sasaki T, Yamamoto Y, Ezaki B, Katsuhara M, Ahn SJ, Ryan PR, Delhaize E, Matsumoto H. A wheat gene encoding an aluminum-activated malate transporter. The Plant Journal. 2004;37:645–653. DOI: 10.1111/j.1365-313X.2003.01991.x\n '},{id:"B248",body:'\n Maron LG, Pineros MA, Guimarães CT, Magalhaes JV, Pleiman JK, Mao C, Shaff J, Belicuas SN, Kochian LV. Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize. The Plant Journal. 2010;61:728–740. DOI: 10.1111/j.1365-313X.2009.04103.x\n '},{id:"B249",body:'\n Ryan PR, Raman H, Gupta S, Horst WJ, Delhaize E. A second mechanism for aluminum resistance in wheat relies on the constitutive efflux of citrate from roots. Plant Physiology. 2009;149:340–351. DOI: 10.1104/pp.108.129155\n '},{id:"B250",body:'\n Magalhaes JV, Liu J, Guimarães CT, Lana UGP, Alves VM, Wang YH, Schaffert RE, Hoekenga OA, Piñeros MA, Shaff JE, Klein PE, Carneiro NP, Coelho CM, Trick HN, Kochian LV. A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum. Nature Genetics. 2007;39:1156–1161. DOI: 10.1038/ng2074\n '},{id:"B251",body:'\n Hoekenga OA, Maron LG, Piñeros MA, Cancado GM, Shaff J, Kobayashi Y, Ryan PR, Dong B, Delhaize E, Sasaki T, Matsumoto H, Yamamoto Y, Koyama H, Kochian LV. AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2006;103:9738–9743. DOI: 10.1073/pnas.0602868103\n '},{id:"B252",body:'\n Liu J, Magalhaes JV, Shaff J, Kochian LV. Aluminum-activated citrate and malate transporters from the MATE and ALMT families function independently to confer Arabidopsis aluminum tolerance. The Plant Journal. 2009;57:389–399. DOI: 10.1111/j.1365-313X.2008.03696.x\n '},{id:"B253",body:'\n Silva-Navas J, Benito C, Téllez-Robledo B, El-Moneim A, Gallego FJ. The ScAACT1 gene at the Qalt5 locus as a candidate for increased aluminum tolerance in rye (Secale cereale L.). Molecular Breeding. 2011;30(2):845–856. DOI: 10.1007/s11032-011-9668-5\n '},{id:"B254",body:'\n Huang J, Xia H, Li Z, Xiong Y, Kong G. Soil aluminum uptake and accumulation by Paspalum notatum. Waste Management Research 2009b;27:668–675. DOI:\n '},{id:"B255",body:'\n Fujii M, Yokosho K, Yamaji N, Saisho D, Yamane M, Takahashi H, Sato K, Nakazono M, Ma JF. Acquisition of aluminium tolerance by modification of a single gene in barley. Nature Communications. 2012;3:713. DOI: 10.1038/ncomms1726\n '},{id:"B256",body:'\n Arenhart RA, Lima JC, Pedron M, Carvalho FE, Silveira JA, Rosa SB, Caverzan A, Andrade CM, Schünemann M, Margis R, Margis-Pinheiro M. Involvement of ASR genes in aluminum tolerance mechanisms in rice. Plant Cell & Environment. 2013b;36:52–67. DOI: 10.1111/j.1365-3040.2012.02553.x\n '},{id:"B257",body:'\n Gao CJ, Xing D, Li LL, Zhang LR. Implication of reactive oxygen species and mitochondrial dysfunction in the early stages of plant programmed cell death induced by ultraviolet-C overexposure. Planta. 2008;227:755–767. DOI: 10.1007/s00425-007-0654-4\n '},{id:"B258",body:'\n Zhu HF, Wang H, Zhu YF, Zou JW, Zhao FJ, Huang CF. Genome-wide transcriptomic and phylogenetic analyses reveal distinct aluminum-tolerance mechanisms in the aluminum-accumulating species buckwheat (Fagopyrum tataricum). BMC Plant Biology. 2015;15:16. DOI: 10.1186/s12870-014-0395-z\n '},{id:"B259",body:'\n Hanikenne M, Talke IN, Haydon MJ, Lanz C, Nolte A, Motte P, Kroymann J, Weigel D, Krämer U. Evolution of metal hyperaccumulation required cis-regulatory changes and triplication of HMA4. Nature. 2008;453(7193):391–395. DOI: 10.1038/nature06877\n '},{id:"B260",body:'\n Kwon H-K, Yokoyama R, Nishitani K. A proteomic approach to apoplastic proteins involved in cell wall regeneration in protoplasts of Arabidopsis suspension-cultured cells. Plant Cell Physiology. 2005;46:843–857. DOI: 10.1093/pcp/pci089\n '},{id:"B261",body:'\n Bhuja P, McLachlan K, Stephens J, Taylor G. Accumulation of 1,3-beta-D-glucans, in response to aluminum and cytosolic calcium in Triticum aestivum. Plant Cell Physiology. 2004;45:543–549. DOI: 10.1093/pcp/pch068\n '},{id:"B262",body:'\n Sivaguru M, Horst WJ, Eticha D, Matsumoto H. Aluminum inhibits apoplastic flow of high-molecular weight solutes in root apices of Zea mays L. Journal of Plant Nutrition and Soil Science. 2006;169:679–690. DOI: 10.1002/jpln.200620603\n '},{id:"B263",body:'\n O’Malley RC, Rodriguez FI, Esch JJ, Binder BM, O’Donnell P, Klee HJ, Bleecker AB. Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato. The Plant Journal. 2005;41:651–659. DOI: 10.1111/j.1365-313X.2004.02331.x\n '},{id:"B264",body:'\n Tian Q, Zhan X, Ramesh S, Gilliham M, Tyerman SD, Zhan W-H. Ethylene negatively regulates aluminium-induced malate efflux from wheat roots and tobacco cells transformed with TaALMT1. Journal of Experimental Botany 2014;65:2415–2426. DOI:\n '},{id:"B265",body:'\n Sun P, Tian Q-Y, Chen J, Zhang W-H. Aluminium-induced inhibition of root elongation in Arabidopsis is mediated by ethylene and auxin. Journal of Experimental Botany. 2010;61:347–356. DOI: 10.1093/jxb/erp306\n '},{id:"B266",body:'\n He H, He L, Gu M. Interactions between nitric oxide and plant hormones in aluminum tolerance. Plant Signaling and Behavior. 2012;7:469–471. DOI: 10.4161/psb.19312\n '},{id:"B267",body:'\n He HY, He LF, Gu MH. Role of microRNAs in aluminum stress in plants. Plant Cell Reports. 2014;33:831–836. DOI: 10.1007/s00299-014-1565-z\n '},{id:"B268",body:'\n Sahrawat KL. Iron toxicity in wetland rice and the role of other nutrients. Journal of Plant Nutrition. 2005;27:1471–1504. DOI: 10.1081/PLN-200025869\n '},{id:"B269",body:'\n Keita A, Yacouba H, Hayde LG, Schultz B. A single-season irrigated rice soil presents higher iron toxicity risk in tropical savannah valley bottoms. Open Soil Science. 2013;3:314–322. DOI: 10.4236/ojss.2013.37036\n '},{id:"B270",body:'\n Onaga G, Edema R, Asea G. Tolerance of rice germplasm to iron toxicity stress and the relationship between tolerance, Fe+2, P and K content in the leaves and roots. Archives of Agronomy and Soil Science. 2013a;59:213–229. DOI: 10.1080/03650340.2011.622751\n '},{id:"B271",body:'\n Roschzttardtz H, Conéjéro G, Divol F, Alcon C, Verdeil JL, Curie C, Mari S. New insights into Fe localization in plant tissues. Frontiers in Plant Science. 2013;4:350. DOI: 10.3389/fpls.2013.00350\n '},{id:"B272",body:'\n Staal M, Cnodder TD, Simon D, Vandenbussche F, Straeten DVD, Verbelen JP, Elzenga T, Vissenberg K. Apoplastic alkalinization is instrumental for the inhibition of cell elongation in the Arabidopsis root by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. Plant Physiology. 2011;155:2049–2055. DOI: 10.1104/pp.110.168476\n '},{id:"B273",body:'\n Quinet M, Vromman D, Clippe A, Bertin P, Lequeux H, Dufey I, Lutts S, Lefèvre I. Combined transcriptomic and physiological approaches reveal strong differences between short and long term response of rice (Oryza sativa) to iron toxicity. Plant Cell & Environment. 2012;35:1837–1859. DOI: 10.1111/j.1365-3040.2012.02521.x\n '},{id:"B274",body:'\n Majerus V, Bertin P, Swenden V, Fortemps A, Lobréaux S, Lutts S. Organ-dependent responses of the African rice to short-term iron toxicity: ferritin regulation and antioxidative responses. Biologia Plantarum 2007;51(2):303–312. DOI: 10.1007/s10535-007-0060-6\n '},{id:"B275",body:'\n Peng XX, Yu XL, Li MQ, Yamauchi M. Induction of peroxidase by Fe 2+ in detached rice leaves. Plant and Soil. 1996;180:159–163. DOI: 10.1007/BF00015422\n '},{id:"B276",body:'\n Fang WC, Wang JW, Lin CC, Kao CH. Iron induction of lipid peroxidation and effects on antioxidative enzyme activities in rice leaves. Plant Growth Regulation. 2001;35(1):75–80. DOI: 10.1023/A:1013879019368\n '},{id:"B277",body:'\n Stein RJ, Lopes SIG, Fett JP. Iron toxicity in field-cultivated rice: contrasting tolerance mechanisms in distinct cultivars. Theoretical and Experimental Plant Physiology. 2014;26:135–146. DOI: 10.1007/s40626-014-0013-3\n '},{id:"B278",body:'\n Wu LB, Shhadi MY, Gregorio G, Matthus E, Becker M, Frei M. Genetic and physiological analysis of tolerance to acute iron toxicity in rice. Rice 2014;7:8. DOI: 10.1186/s12284-014-0008-3\n '},{id:"B279",body:'\n Dufey I, Mathieu AS, Draye X, Lutts S, Bertin P. Construction of an integrated map through comparative studies allows the identification of candidate regions for resistance to ferrous iron toxicity in rice. Euphytica. 2015a;203:59–69. DOI: 10.1007/s10681-014-1255-5\n '},{id:"B280",body:'\n Fukuda A, Shiratsuchi H, Fukushima A, Yamaguchi H, Mochida H, Terao T, Ogiwara H. Detection of chromosomal regions affecting iron concentration in rice shoots subjected to excess ferrous iron using chromosomal segment substitution lines between Japonica and Indica. Plant Production Science. 2012;15:183–191. DOI: 10.1626/pps.15.183\n '},{id:"B281",body:'\n Shimizu A. QTL analysis of genetic tolerance to iron toxicity in rice (Oryza sativa L.) by quantification of bronzing score. Journal of New Seeds. 2009;10(3):171–179. DOI: 10.1080/15228860903064989\n '},{id:"B282",body:'\n Yuan F, Yang H, Xue Y, Kong D, Ye R, Li C, Zhang J, Theprungsirikul L, Shrift T, Krichilsky B, Johnson DM, Swift GB, He Y, Siedow JN, Pei ZM. OSCA1 mediates osmotic-stress evoked Ca2+ increases vital for osmosensing in Arabidopsis. Nature. 2014; 514(7522):367-371. DOI: 10.1038/nature13593\n '}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Geoffrey Onaga",address:null,affiliation:'
National Crops Resources Research Institute, Kampala, Uganda
Erfurt University of Applied Sciences, Faculty of Landscape Architecture, Horticulture and Forestry, Erfurt, Germany
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1. Introduction
Drosophila suzukii Matsumura (Diptera: Drosophilidae) is classified by the European and Mediterranean Plant Protection Organization (EPPO) as an emerging and invasive pest [1]. This insect is native to east and southeast Asia, although its real geographical origin is still discussed [2]. It has been introduced in several Hawaiian Islands [2], North America [3], Europe [4], and recently in Iran [5], Argentina [6], and later Chile [7], reflecting its high adaptive capacity. Its relevance is due to high economic importance because of the severity of the damage it causes in various productive crops [8, 9, 10, 11, 12, 13, 14, 15]. It is a very polyphagous pest, with more than 50 hosts reported in literature [16].
In contrast with Drosophila melanogaster, the spotted wing fruit fly develops in mature and undamaged fruits, injecting its eggs into fruits when they preferably begin to turn color [17]. The larvae develop inside the fruits, finally pupating on or inside them [15, 17]. If there is not enough healthy fruit, it can also attack fallen or damaged fruit [18].
Since only 2 years ago its presence was reported in Chile, until now there was no corroboration of its productive impact in the country. At the same time, for the design of the management proposals, there was only external literature; however, the objective of this work was to monitor the economic damage caused by the plague in cherry and blueberry orchards and also evaluate the control achieved with insecticides of different chemical groups: alpha-cypermethrin, acetamiprid, chlorantraniliprole, cyantraniliprole, emamectin benzoate, lambda- and gamma-cyhalothrin, spinetoram, and spinosad.
The possible repellent effect of garlic and chili-based products, as well as an extract of Chenopodium ambrosioides, was also investigated.
2. Methodology
2.1 Fruit damage monitoring
Five commercial orchards were selected from localities Cachapoal and Culenar, Ñuble Region, in areas with positive D. suzukii detections since 2017. From the newly formed fruit state, in September 2019, cherry orchards and blueberry orchards were monitored three times per week until January 2020. Production areas of more than 10 hectares were addressed and with at least 10 years of operation. The orchards correspond to conventional production, with drip technified irrigation and with similar agricultural management conditions with each other.
Three varieties of cherries (Lapins, Santina, and Regina) and two of blueberries (Brigitta and Elliot), as also material from live fences of Aristotelia chilensis, Rubus ulmifolius, and Prunus cerasifera, were included in this monitoring. From each substrate and on each occasion, 100 random fruits were collected, and some insect individuals were selected for analysis with traditional taxonomy and/or PCR analysis for the larva and pupa states extracted from the samples. The monitoring record was carried in parallel to the climate record of temperature and relative humidity.
At harvest, losses associated with the pest were estimated in orchard and prepackaged fruit inspection.
2.2 Efficacy test
Once the start of the attack of D. suzukii on fruits was determined, the cherry orchard with the highest severity of attack was selected to perform efficacy studies. In this step, a cherry orchard of cv. Regina (1200 plants/ hectare) was used. Insecticides from five chemical groups were compared [19]: group 3A (pyrethroids: alpha-cypermethrin and lambda- and gamma-cyhalothrin), group 4A (neonicotinoids: acetamiprid), group 5 (spinosyns: spinetoram and spinosad), group 6 (avermectins: emamectin benzoate), and group 28 (diamides: chlorantraniliprole and cyantraniliprole).
The treatments evaluated were Mageos® (15% w/w alpha-cypermethrin dispersible granulated formulation; 15 g of commercial product/100 L) (Basf Chile S.A); Bull® (6.12% w/v gamma-cyhalothrin encapsulated suspension; 10 mL of commercial product/100 L) (FMC Chemical Chile Ltd); Karate® Zeon (5% w/v lambda-cyhalothrin encapsulated suspension; 20 mL of commercial product/100 L) (Syngenta S.A); Mospilan® (20% w/w acetamiprid soluble powder; 50 g/100 L) (Basf Chile S.A); Success® 48 (45% w/v spinosad concentrated suspension; 14 g/100 L); Delegate® (25% w/w spinetoram concentrated suspension; 16 g/100 L); Proclaim® Forte (5% w/w emamectin benzoate dispersible granulate: 30 g/100 L) (Syngenta S.A); Coragen® (20% w/v chlorantraniliprole concentrated suspension; 20 mL/100 L) (FMC Chemical Chile Ltd); and Exirel® (10% w/v cyantraniliprole suspoemulsion; 75 mL/100 L) (FMC Chemical Chile Ltd). A control without insecticide was included.
A completely randomized design was used, with four repetitions of each treatment. The experimental unit corresponded to groups of 10 plants of the same row treated with 4 isolation plants between them.
All applications were performed once, on November 15 (when fruit begins to take color), with a conventional hydraulic sprayer (Line Patasa 2000) with 1800 L of water per hectare. A control treatment without insecticide applications was considered.
Two parameters of relevance were evaluated: the incidence of damage (established as mean of fruits damaged by the pest) and the severity of the damage (wound or presence of live larvae) on samples of 100 fruits collected randomly within each experimental unit. Fruits were reviewed at 3, 5, 7, 10, 12, and 14 days after application (DAA). The cutoff criterion to stop evaluations was unified when the incidence of damage was equal to or greater than 20%.
The data obtained from field and laboratory measurements was subjected to analysis of variance (ANOVA) by taking appropriate transformations. Mean comparisons in significant ANOVAs were performed with a Tukey’s test. Statistical analyses were conducted using the software Minitab®16.1.0 (Minitab Inc.).
2.3 Repellency test
In a cherry orchard of var. Regina located in the town of Culenar, the possible effect of repellency (expressed as a lower incidence of eggs laid of D. suzukii on cherries) achieved by the use of two commercial products based on natural compounds, Amarex® (Captiva® Prime) which is formulated by 7.60% v/v capsicum oleoresin extract, 23.40% v/v garlic oil, and 55% v/v canola oil (Gowan Chile SpA) and Requiem® Prime which is formulated by 9.1% w/v α-terpinene, 3.4% w/v p-cymene, and 2.73% w/p d-limonene (Bayer S.A), was studied. Both were applied three times every 5 days as fruit starts to change color.
Treatment with Amarex® was applied at 200 mL/100 L, and treatment with Requiem® Prime was applied at 240 mL/100 L, using a conventional hydraulic sprayer (Line Patasa 2000) with 1800 L of water per hectare. One check control using only water treatment was included.
All treatment had four repetitions of 10 plants, randomly drawn inside the orchard. Evaluations were performed at 3 days between applications and at 3 and 7 days after the last application.
On each occasion the frequency of egg of D. suzukii laid in fruits was evaluated, in samples of 100 fruits collected at random.
After each evaluation involved in the present work, the destruction of the contaminated material was carried out via freezing at -30C for 5 days.
3. Results
All monitored hosts showed damage and development of immature states of the pest from the beginning of fruit maturity. The above adds A. chilensis, a Chilean native plant, and Prunus cerasifera (Figure 1) to the list of secondary hosts of the pest.
Figure 1.
Inmature stages of D. suzukii collected from fruits of Prunus cerasifera.
The period of greatest incidence of the pest was at the beginning of fruit color breakage and increases as it approaches maturity. Development persists in remnants of the orchard after harvest (Figures 2 and 3).
Figure 2.
Percentage (%) of cherry fruits attacked by D. suzukii separated by cultivar.
Figure 3.
Percentage (%) of blueberry fruits attacked by D. suzukii separated by cultivar.
There were no indications of preference of attack of one fruit variety over another nor signs of preference among fruit species when comparing the incidence between cherry trees and blueberries in similar conditions. The foregoing could vary according to different pesticides or nutritional programs or driving management used among these crops, but at least the records collected suggest that similar precautions should be handled in these fruit trees.
There was no rainfall in the period of pest incidence, and daily temperatures ranged from 6 to 36°C. Relative humidity varied from 40 to 65%, that is, adjusted to favorable climatic requirements for the development of the pest [20]. The area of cherries in Chile currently exceeds 27,000 hectares, concentrated in areas that have favorable climatic conditions for this pest, a situation that is even more marked in the case of blueberry production.
Since Chile is a country focused on the export of fruits, the damages observed until now can be categorized in two categories: complete loss of the fruit (Figure 4) or loss of quality due to deterioration or damage to the skin of the fruit (Figure 5).
Figure 4.
Losses caused in cherry trees by massive attack of D. suzukii.
Figure 5.
Cherries showing damage by egg laying attempts.
In the monitored cases, for cherries, the first category reached between 10% and15% of the production (approximately 10% Santina, 12% Lapins, and 15% Regina), that is, the equivalent of 1, 2.16, and 2.7 ton/ha. Economically speaking, it is a loss equivalent to 5,000–17,550 USD/hectare. The second item is still difficult to quantify. The second item was estimated at 1–2%.
In the case of blueberries, the percentage of compromised fruit was close to 8%, which could increase as the plague increases its local population. This corresponds to the equivalent of 1–1.5 ton/ha or a loss equivalent to 4,000 USD/hectare.
In fruits of live fences (A. chilensis, R. ulmifolius, and P. cerasifera), at the end of the season, the recorded frequency was greater than 60%, possibly since they do not receive pesticide treatments and not necessarily because there is a greater attraction or preference to those substrates. Unfortunately, at least two of these three species are widely distributed in the country, such as ornamental plants or live fences, which contribute to the spread of the pest.
Secondary damage due to attempts to lay eggs occurred in some sectors that received certain insecticidal treatments and allowed the fruit to be redirected to the domestic market, although at a significative lower return value than expected in an export fruit. Concordant with what happened in other countries, greater complexity is expected from the point of view of the increase of residues present in fruit due to the management of this pest [21].
Control delivered by the microencapsulated pyrethroid treatments, both spinosyns, and cyantraniprole was highly efficient not only in reducing the incidence of infestation (Figure 6) but also in decreasing the severity of the attack (Figure 7).
Figure 6.
Mean percentage (%) of fruits damaged by D. suzukii days after each insecticide treatment.
Figure 7.
Mean of wound/or live larvae of D. suzukii in fruits days after each insecticide treatment.
Spinosyns and some pyrethroids such as l-cyhalothrin are indicated with high efficacy in the control of damage of this pest, at short intervals of application to avoid egg postures [22].
In the case of acetamiprid, emamectin benzoate, and chlorantraniliprole, these showed weaker insecticidal action than the previous group, considering that the control action was only expressed once the fruit was affected by the pest. Even so, because they show a significant decrease in the incidence of damage for an approximate period of 5 days, future work may focus on complementing these mechanisms of action with others (such as repellents) that allow reducing the severity of the damage. These results are consistent with what was raised by specialists in the United States [23]. Nevertheless, despite being promising in management, the high rate of dispersal, reproduction, and adaptation of the pest is consistent with the high risk of economic damage reported globally [24].
On the other hand, although the history of efficient biological control has been weak [23], several authors agree on the need to advance in the integral knowledge of the management of this insect, including predators, parasitoids, repellents, insecticides, and cultural management [25, 26, 27, 28, 29, 30].
Regarding the effect granted using products based on natural compounds, both Requiem® Prime and Amarex® demonstrated at least the decrease in the severity of attack of the pest under conditions of use in the orchard (Figure 8). However, it should be considered that the only use of these treatments may not be enough to reduce the economic impact when faced with increased D. suzukii pressure. More studies should be realized about their possible contribution inserted in a program or in mixture with conventional insecticides in order to contribute to complement mode of action.
Figure 8.
Severity of attack (frequency of D. suzukii egg position).
The mode of action as insecticide reported for Requiem® Prime are explained because the active ingredient cause degradation of soft insect cuticles results in the disruption of insect mobility and respiration [31]. In the case of Amarex®, its action would also be explained by destruction of membranes added to the repellent effect [32].
4. Conclusions
The results of the monitoring of damage in fruits and its evolution during the season reflect that the potential risk of the plague in a country with the productive reality of Chile is high and that locally D. suzukii has found new shelters that allow it to spread and maintain its development. Although, in the search for its control, adequate tools were determined to reduce its damage, a greater understanding of the mechanism of action of each product is required in order to position it properly, in order to reduce the potential damage of the insect.
Given the high-quality requirement presented by the export fruit, there is a high risk of selection of less sensitive individuals of the pest, and those cases of violations of the maximum limits of pesticide residues occur due to an overheating of applications. For this reason, future work should consider other integrated management edges. In this line, it is necessary to investigate locally the use of possible mixtures of repellents with insecticides, the use of biological controllers in parallel to a management program, and the use of mass capture traps.
For now, two formulated products (Requiem® Prime and Amarex®) have promising results to achieve a lower severity of damage without adding residues to the fruit, which can be complementary to the use of insecticides aimed primarily at the management of adults of this pest.
Of the insecticides compared, the control delivered by cyantraniliprole and both spinosyns stands out. All microencapsulated pyrethroids showed stable control for at least 7 days. Acetamiprid, emamectin benzoate, and chlorantraniliprole were not efficient in reducing the attempt to lay eggs but were enough to decrease the incidence of damage. Therefore, in the short term, in Chile there are adequate tools for the management of this insect, but adequate use should be provided in order to preserve food safety.
Caution should also be taken with the selection of less sensitive individuals of the pest, because, due to the characteristics of the species, the expression of resistance to insecticides can enhance their economic damage.
Acknowledgments
The author personally thanks Juan Carlos Ríos, Ricardo Larral, Alejandro Salas M., and Fernando Jofre. Acknowledgments are also expressed to SAG officials Rodrigo Astete, Marco Muñoz, Ruth Castro, Claudio Moore T., Nayadeth Álvarez, Oscar Rojas, and Marta Solís.
The study was funded exclusively by AgriDevelopment Ltd.
Conflict of interest
The author declares no conflict of interest in the delivery information.
\n',keywords:"fruit fly, insecticides, Drosophilidae, invasive pest, economic losses",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/71435.pdf",chapterXML:"https://mts.intechopen.com/source/xml/71435.xml",downloadPdfUrl:"/chapter/pdf-download/71435",previewPdfUrl:"/chapter/pdf-preview/71435",totalDownloads:993,totalViews:0,totalCrossrefCites:1,dateSubmitted:"January 16th 2020",dateReviewed:"February 9th 2020",datePrePublished:"March 13th 2020",datePublished:"September 23rd 2020",dateFinished:"March 13th 2020",readingETA:"0",abstract:"The spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae) is endemic in Asia. This species was first reported in Chile in early 2017 in the region of La Araucanía, Los Ríos, and Los Lagos, but it has dispersed to other regions of the south-central area of the country, currently being in the category of plague present with restricted distribution. D. suzukii is a polyphagous pest, and it infests a wide range of fruit crops, including grapes, cherries, as well as an increasing number of wild fruits. Chile is the main cherry-producing country in the south of the world, providing mainly to the Asian market, so, although the pest is not a quarantine problem for this destination of the fruit, its potential damage is high due to the decrease in yield, fruit deterioration, and increased pre- and postharvest rot. The local productive reality also provides various hosts to the plague, allowing its expression and adaptation, for example, in blueberry orchards. This report summarizes the first signs of economic damage and explores the first results of control delivered by various insecticides: alpha-cypermethrin, acetamiprid, chlorantraniliprole, cyantraniliprole, emamectin benzoate, lambda- and gamma-cyhalothrin, spinetoram, and spinosad. Also, repellent effect of other compounds was studied.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/71435",risUrl:"/chapter/ris/71435",signatures:"Karina Buzzetti Morales",book:{id:"9698",type:"book",title:"Invasive Species",subtitle:"Introduction Pathways, Economic Impact, and Possible Management Options",fullTitle:"Invasive Species - Introduction Pathways, Economic Impact, and Possible Management Options",slug:"invasive-species-introduction-pathways-economic-impact-and-possible-management-options",publishedDate:"September 23rd 2020",bookSignature:"Hamadttu El-Shafie",coverURL:"https://cdn.intechopen.com/books/images_new/9698.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-78985-850-1",printIsbn:"978-1-78985-849-5",pdfIsbn:"978-1-83880-431-2",isAvailableForWebshopOrdering:!0,editors:[{id:"192142",title:"Dr.",name:"Hamadttu",middleName:null,surname:"El-Shafie",slug:"hamadttu-el-shafie",fullName:"Hamadttu El-Shafie"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"214596",title:"Dr.",name:"Karina",middleName:null,surname:"Buzzetti",fullName:"Karina Buzzetti",slug:"karina-buzzetti",email:"karinabuzzetti@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Methodology",level:"1"},{id:"sec_2_2",title:"2.1 Fruit damage monitoring",level:"2"},{id:"sec_3_2",title:"2.2 Efficacy test",level:"2"},{id:"sec_4_2",title:"2.3 Repellency test",level:"2"},{id:"sec_6",title:"3. Results",level:"1"},{id:"sec_7",title:"4. Conclusions",level:"1"},{id:"sec_8",title:"Acknowledgments",level:"1"},{id:"sec_11",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'European and Mediterranean Plant Protection Organization (EPPO). Global Database. EPPO A2 List of pests recommended for regulation as quarantine pests (version 19/09). Available from: https://www.eppo.int/ACTIVITIES/plant_quarantine/A2_list [Accessed: 05 January 2020]'},{id:"B2",body:'Hauser M. A historic account of the invasion of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in the continental United States, with remarks on their identification. Pest Management Science. 2011;67(11):1352-1357'},{id:"B3",body:'Burrack HJ, Smith JP, Pfeiffer DG, Koeher G, Laforest J. Using volunteer-based networks to track Drosophila suzukii (Diptera: Drosophilidae) an invasive pest of fruit crops. Journal of Integrated Pest Management. 2012;3(4):B1-B5'},{id:"B4",body:'Cini A, Ioriatti C, Anfora G. A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management. Bulletin of Insectology. 2012;65(1):149-160'},{id:"B5",body:'Mehrdad P-A, Gilasian E, Kayhanian AA. Spotted wing drosophila, Drosophila suzukii (Matsumura) (Dip.: Drosophilidae), an invasive fruit pest new to the Middle East and Iran. Drosophila Information Service. 2015;98:59-60'},{id:"B6",body:'ChiaHua L, Mottern JL, Walsh GC, Buffington ML. New record for the invasive spotted wing drosophila, Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) in Anillaco, western Argentina. Proceedings of the Entomological Society of Washington. 2017;119(1):146-150'},{id:"B7",body:'Servicio Agrícola y Ganadero (SAG) Chile. Plagas relevantes presentes. Drosophila suzukii. 2019. Available from: https://www.sag.gob.cl/ambitos-de-accion/drosophila-suzukii [Accessed: 05 January 2020]'},{id:"B8",body:'Grassi A, Palmie L, Giongo L. New pests of the small fruits in Trentino. (Nuovo fitofago per i piccoli frutti in Trentino.). Terra Trentina. 2009;55(10):19-23'},{id:"B9",body:'Bolda M, Goodhue RE, Zalom FG. Spotted wing drosophila: Potential economic impact of a newly established pest. Agricultural and Resource Economics. 2010;13(3):5-8'},{id:"B10",body:'Goodhue RE, Bolda M, Farnsworth D, Williams JC, Zalom FG. Spotted wing drosophila infestation of California strawberries and raspberries: Economic analysis of potential revenue losses and control costs. Pest Management Science. 2011;67(11):1396-1402'},{id:"B11",body:'De Ros G, Anfora G, Grassi A, Ioriatti C. The potential economic impact of Drosophila suzukii on small fruits production in Trentino (Italy). IOBC-WPRS Bulletin. 2013;91:317-321'},{id:"B12",body:'Mazzi D, Bravin E, Meraner M, Finger R, Kuske S. Economic impact of the introduction and establishment of Drosophila suzukii on sweet cherry production in Switzerland. Insects. 2017;8(1):18'},{id:"B13",body:'De Ros G, Conci S, Pantezzi T, Savini G. The economic impact of invasive pest Drosophila suzukii on berry production in the province of Trento, Italy. Journal of Berry Research. 2015;5(2):89-96'},{id:"B14",body:'Wiman NG, Dalton DT, Anfora G, Biondi A, Chiu JC, Daane KM, et al. Drosophila suzukii population response to environment and management strategies. Journal of Pest Science. 2016;89(3):653-665'},{id:"B15",body:'Kanzawa T. Studies on Drosophila suzukii Mats. Kofu, Yamanashi agricultural Experiment Station. Abstract in the Review of Applied Entomology. 1939;29:622'},{id:"B16",body:'Centre for Agricultural Bioscience International (CABI). D. suzukii, host/species affected. 2020. Available from: https://www.cabi.org/isc/datasheet/109283#tohostsOrSpeciesAffected [Accessed: 05 January 2020]'},{id:"B17",body:'Woltz JM, Lee JC. Pupation behavior and larval and pupal biocontrol of Drosophila suzukii in the field. Biological Control. 2017;110:62-69'},{id:"B18",body:'Burrack HJ, Fernandez GE, Spivey T, Kraus DA. Variation in selection and utilization of host crops in the field and laboratory by Drosophila suzukii Matsumura (Diptera: Drosophilidae), an invasive frugivore. Pest Management Science. 2013;69(10):1173-1180'},{id:"B19",body:'Insecticide Action Committee (IRAC). 2019. IRAC Mode of Action Classification Scheme, Issued June 2019. p. 30'},{id:"B20",body:'Tochen S, Dalton DT, Wiman NG, Hamm C, Shearer PW, Walton VM. Temperature-related development and population parameters for Drosophila suzukii (Diptera: Drosophilidae) on cherry and blueberry. Environmental Entomology. 2014;43:501-510'},{id:"B21",body:'Haviland DR, Beers EH. Chemical control programs for Drosophila suzukii that comply with international limitations on pesticide residues for exported sweet cherries. Journal of Integrated Pest Management. 2012;3(2):F1-F6'},{id:"B22",body:'Torné M, Abad R, Jacquet V. Spinosines®, una herramienta imprescindible para el control de Drosophila suzukii Matsumara. Phytoma. 2014;265:26-28'},{id:"B23",body:'Asplen MK, Anfora G, Biondi A, Choi DS, Chu D, Daane KM, et al. Invasion biology of spotted wing drosophila (Drosophila suzukii): A global perspective and future priorities. Journal of Pest Science. 2015;88(3):469-494'},{id:"B24",body:'Bolda MP, Goodhue RE, Zalon FG. Spotted wing drosophila: Potential economic impact of a newly established pest. The Giannini Foundation of Agricultural Economics. 2010;13:5-8'},{id:"B25",body:'Walsh DB, Bolda MP, Goodhue RE, Dreves AJ, Lee J, Bruck DJ, et al. Drosophila suzukii (Diptera: Drosophilidae): Invasive pest of ripening soft fruit expanding its geographic range and damage potential. Journal of Integrated Pest Management. 2011;2:G1-G7'},{id:"B26",body:'Schlesener D, Wollmann J, Pazini J, Grützmacher A, Mello Garcia F. Effects of insecticides on adults and eggs of Drosophila suzukii (Diptera, Drosophilidae). Revista Colombiana de Entomologia. 2017;43(2):208-214'},{id:"B27",body:'Rossi M, Grassi A, Dalton DT, Miller B, Ouantar M, Loni A, et al. First field records of Pachycrepoideus vindemiae as a parasitoid of Drosophila suzukii in European and Oregon small fruit production areas. Entomologia. 2013;1:e3'},{id:"B28",body:'Rossi M, Buffington M, Daane KM, Dalton DT, Grassi A, Kacar G, et al. Host stage preference, efficacy and fecundity of parasitoids attacking Drosophila suzukii in newly invaded areas. Biological Control. 2015;84:28-35'},{id:"B29",body:'Caprile J, Flint ML, Bolda MP, Grant JA, Van Steenwyk R, Haviland D. 2013. Spotted wing Drosophila (Drosophila suzukii). University of California-IPM online. Available from: http://www.ipm.ucdavis.edu/EXOTIC/drosophila.html. [Accessed: 07 January 2020]'},{id:"B30",body:'Cormier D, Veilleux J, Firlej A. Exclusion net to control spotted wing drosophila in blueberry fields. IOBC-WPRS Bulletin. 2015;109:181-184'},{id:"B31",body:'Certis USA 2019. Requiem® Prime. Available from: https://www.certisusa.com/pest_management_products/bioinsecticides/requiemprime [Accessed: 05 January 2020]'},{id:"B32",body:'Gowan Company. 2020. Captiva® Prime. Available from: https://www.gowanco.com/products/captiva-prime [Accessed: 11 January 2020]'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Karina Buzzetti Morales",address:"karinabuzzetti@agridev.cl",affiliation:'
Consultant AgriDevelopment Ltd, Santiago, Chile
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She worked as faculty member in the departments of Microbiology, Medical College, Srinagar, Kashmir; Medical College, Jammu; Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Kashmir, and is currently holding post of Professor at PGIMER, Chandigarh, India. In recognition of her diagnostic and research capabilities, she was awarded many National level Academic Awards and British Council Fellowship. She is fellow of the Royal Society of Tropical Medicine and Hygiene; Fellow, National Academy of Medical Sciences, India and member of many National and International Academic Societies. 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IMPORTANT: You must be a member or grantee of the listed funders in order to apply for their Open Access publication funds. Do not attempt to contact the funders if this is not the case.
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Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. He has contributed in stochastic estimation of control area especially, in the Multiple Target Tracking and Interactive Multiple Model (IMM) research, Ball & Beam Control Problem, Robotics, Levitation Control. He has contributed in developing Algorithms for Fingerprint Matching, Computer Vision and Face Recognition. He has been supervising Pattern Recognition, Formal Languages and Distributed Processing projects for several years. He has reviewed many books on Management, Computer Science. Currently, he is an active and permanent reviewer for many international conferences and symposia and the program committee member for many international conferences.\nIn teaching he has taught the core computer science subjects like, Digital Design, Real Time Embedded System Programming, Operating Systems, Software Engineering, Data Structures, Databases, Compiler Construction. 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Today, the term “Systemic speech-and-language underdevelopment (SLU)” has firmly established in Russian science and practice, implying a complex developmental disorder of speech and language in children with a primary normal hearing and a conserved intellect, in which the main components of the language system are violated: vocabulary, grammar, phonetics, and, as a consequence, dialogic and monologic speech. Traditionally, a differentiated level-by-level analysis of the speech and language abilities of children is used. The variability of the manifestations and severity of speech-and-language disorders were initially systematized and characterized in four levels of underdevelopment: from the complete absence of phrase speech to the availability of simple and complex sentences with lexico-grammatical errors. Effective algorithms of speech therapist work with SLU are introduced. The effectiveness of the application of these models and algorithms on the material of various language groups is proved.",book:{id:"5957",slug:"advances-in-speech-language-pathology",title:"Advances in Speech-language Pathology",fullTitle:"Advances in Speech-language Pathology"},signatures:"Tatiana Tumanova and Tatiana Filicheva",authors:[{id:"204529",title:"Dr.",name:"Tatiana Volodarovna",middleName:null,surname:"Tumanova",slug:"tatiana-volodarovna-tumanova",fullName:"Tatiana Volodarovna Tumanova"},{id:"208704",title:"Dr.",name:"Tatiana Borisovna",middleName:null,surname:"Filicheva",slug:"tatiana-borisovna-filicheva",fullName:"Tatiana Borisovna Filicheva"}]},{id:"36452",doi:"10.5772/38931",title:"Qualitative Research Methods in Psychology",slug:"qualitative-research-methods-in-psychology",totalDownloads:35851,totalCrossrefCites:13,totalDimensionsCites:17,abstract:null,book:{id:"1997",slug:"psychology-selected-papers",title:"Psychology",fullTitle:"Psychology - Selected Papers"},signatures:"Deborah Biggerstaff",authors:[{id:"123274",title:"Dr.",name:"Deborah",middleName:null,surname:"Biggerstaff",slug:"deborah-biggerstaff",fullName:"Deborah Biggerstaff"}]},{id:"56560",doi:"10.5772/intechopen.70235",title:"The Role of Speech and Language Therapist in Autism Spectrum Disorders Intervention – An Inclusive Approach",slug:"the-role-of-speech-and-language-therapist-in-autism-spectrum-disorders-intervention-an-inclusive-app",totalDownloads:2332,totalCrossrefCites:2,totalDimensionsCites:16,abstract:"The chapter describes the possibilities of involving a speech-language therapist in the assessment of the pragmatic level of communication in autism spectrum disorders (ASD), where one of the most frequently impaired areas is communication pragmatics. These difficulties lead to a disruption of social interaction, which might be one of the obstacles to speech-language intervention in these children. The text is based on an originally developed testing material aimed at selected pragmatic-oriented communication situations relating to everyday activities and real life. Based on a comparison of domestic and international resources in this area, as well as mediated and own empirical experience, our assessment approach is based on the conclusion that pragmatics can be understood in different contexts and perspectives. The text presents the results of a partial survey comparing the performance of children with ASD and children with typical development. The assessment focused on the children’s election of the correct picture of a pair of pictures that represent usual communication and social situations. The results of the research suggest fewer incorrect responses in children with ASD and in different areas compared with children with typical development. However, the results of a qualitative analysis indicate a necessity to expand the assessment of communication pragmatics by adding an individually specific qualitative analysis of children’s performance.",book:{id:"5957",slug:"advances-in-speech-language-pathology",title:"Advances in Speech-language Pathology",fullTitle:"Advances in Speech-language Pathology"},signatures:"Kateřina Vitásková and Lucie Kytnarová",authors:[{id:"203061",title:"Associate Prof.",name:"Kateřina",middleName:null,surname:"Vitásková",slug:"katerina-vitaskova",fullName:"Kateřina Vitásková"},{id:"212035",title:"MSc.",name:"Lucie",middleName:null,surname:"Kytnarová",slug:"lucie-kytnarova",fullName:"Lucie Kytnarová"}]}],mostDownloadedChaptersLast30Days:[{id:"73271",title:"Social Media and Its Effects on Beauty",slug:"social-media-and-its-effects-on-beauty",totalDownloads:2991,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Beauty is concerned with physical and mental health as both are intimately related. Short-term decisions to alter one’s body structure irrespective of genetic, environmental, occupational and nutritional needs can leave medium- and long-term effects. This chapter analyzes the role of social media and its effects on the standards of beauty. The researchers have summarized the literature on how social media plays a role in affecting beauty trends, body image and self-esteem concerns. There is support that social media affects individuals negatively, in pushing them to engage in life threatening beauty trends due to social compliance and acceptance in society. The aim was to review social networking sites’ impact on perception of standards of beauty and newer unrealistic trends gaining popularity that could alter opinions and also cause harm to individuals in the long run. This is an emerging area of research that is of high importance to the physical and mental health in the beauty, health and hospitality industry with the latter being manifested in depression, anxiety and fear of non-acceptability and being seen as a social gauche.",book:{id:"7811",slug:"beauty-cosmetic-science-cultural-issues-and-creative-developments",title:"Beauty",fullTitle:"Beauty - Cosmetic Science, Cultural Issues and Creative Developments"},signatures:"Mavis Henriques and Debasis Patnaik",authors:[{id:"320016",title:"Ph.D. Student",name:"Mavis",middleName:"Lilian",surname:"Henriques",slug:"mavis-henriques",fullName:"Mavis Henriques"},{id:"320978",title:"Dr.",name:"Debasis",middleName:null,surname:"Patnaik",slug:"debasis-patnaik",fullName:"Debasis Patnaik"}]},{id:"60564",title:"Ageing Process and Physiological Changes",slug:"ageing-process-and-physiological-changes",totalDownloads:6884,totalCrossrefCites:16,totalDimensionsCites:31,abstract:"Ageing is a natural process. Everyone must undergo this phase of life at his or her own time and pace. In the broader sense, ageing reflects all the changes taking place over the course of life. These changes start from birth—one grows, develops and attains maturity. To the young, ageing is exciting. Middle age is the time when people notice the age-related changes like greying of hair, wrinkled skin and a fair amount of physical decline. Even the healthiest, aesthetically fit cannot escape these changes. Slow and steady physical impairment and functional disability are noticed resulting in increased dependency in the period of old age. According to World Health Organization, ageing is a course of biological reality which starts at conception and ends with death. It has its own dynamics, much beyond human control. However, this process of ageing is also subject to the constructions by which each society makes sense of old age. In most of the developed countries, the age of 60 is considered equivalent to retirement age and it is said to be the beginning of old age. In this chapter, you understand the details of ageing processes and associated physiological changes.",book:{id:"6381",slug:"gerontology",title:"Gerontology",fullTitle:"Gerontology"},signatures:"Shilpa Amarya, Kalyani Singh and Manisha Sabharwal",authors:[{id:"226573",title:"Ph.D.",name:"Shilpa",middleName:null,surname:"Amarya",slug:"shilpa-amarya",fullName:"Shilpa Amarya"},{id:"226593",title:"Dr.",name:"Kalyani",middleName:null,surname:"Singh",slug:"kalyani-singh",fullName:"Kalyani Singh"},{id:"243264",title:"Dr.",name:"Manisha",middleName:null,surname:"Sabharwal",slug:"manisha-sabharwal",fullName:"Manisha Sabharwal"}]},{id:"27237",title:"Emotional Intelligence",slug:"emotional-intelligence",totalDownloads:5728,totalCrossrefCites:6,totalDimensionsCites:9,abstract:null,book:{id:"679",slug:"emotional-intelligence-new-perspectives-and-applications",title:"Emotional Intelligence",fullTitle:"Emotional Intelligence - New Perspectives and Applications"},signatures:"Adrian Furnham",authors:[{id:"85492",title:"Prof.",name:"Adrian",middleName:null,surname:"Furnham",slug:"adrian-furnham",fullName:"Adrian Furnham"}]},{id:"70731",title:"Theoretical Perspective of Traditional Counseling",slug:"theoretical-perspective-of-traditional-counseling",totalDownloads:1582,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"This chapter discusses the theoretical perspective of traditional counseling from an African context. Traditional counseling involves a broad perspective that enhances learning for transformation and integration of sociocultural values that are peculiar to each human society. A cursory review of the literature suggests that the concept of traditional counseling is rooted in traditional systems of knowledge and sociocultural customs and practices, and it promotes a collective approach to problem identification, resolution, and management. The traditional counseling process centers on four aspects: traditional counselor, client, family, and community. The key elements that inform the theoretical framework of traditional counseling from an African perspective are: cultural context, collective belief system, and initiation rituals Traditional systems of knowledge deemed essential for each generation are passed on successively to the next generation by elderly people who do not only have the necessary wisdom and experience, but are also adorned with social competences and skills.",book:{id:"9136",slug:"counseling-and-therapy",title:"Counseling and Therapy",fullTitle:"Counseling and Therapy"},signatures:"Hector Chiboola",authors:[{id:"314172",title:"Prof.",name:"Hector",middleName:null,surname:"Chiboola",slug:"hector-chiboola",fullName:"Hector Chiboola"}]},{id:"55388",title:"Beauty, Body Image, and the Media",slug:"beauty-body-image-and-the-media",totalDownloads:7678,totalCrossrefCites:5,totalDimensionsCites:12,abstract:"This chapter analyses the role of the mass media in people’s perceptions of beauty. We summarize the research literature on the mass media, both traditional media and online social media, and how they appear to interact with psychological factors to impact appearance concerns and body image disturbances. There is a strong support for the idea that traditional forms of media (e.g. magazines and music videos) affect perceptions of beauty and appearance concerns by leading women to internalize a very slender body type as ideal or beautiful. Rather than simply being passive recipients of unrealistic beauty ideals communicated to them via the media, a great number of individuals actually seek out idealized images in the media. Finally, we review what is known about the role of social media in impacting society’s perception of beauty and notions of idealized physical forms. Social media are more interactive than traditional media and the effects of self‐presentation strategies on perceptions of beauty have just begun to be studied. This is an emerging area of research that is of high relevance to researchers and clinicians interested in body image and appearance concerns.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Jennifer S. Mills, Amy Shannon and Jacqueline Hogue",authors:[{id:"202110",title:"Dr.",name:"Jennifer S.",middleName:null,surname:"Mills",slug:"jennifer-s.-mills",fullName:"Jennifer S. Mills"}]}],onlineFirstChaptersFilter:{topicId:"21",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82260",title:"Psychometric Analysis of an Instrument to Study Retention in Engineering",slug:"psychometric-analysis-of-an-instrument-to-study-retention-in-engineering",totalDownloads:4,totalDimensionsCites:0,doi:"10.5772/intechopen.105443",abstract:"Although engineering programs admit highly qualified students with strong academic credentials, retention in engineering remains lower than most other programs of study. Addressing retention by modeling student success shows promise. Instruments incorporating noncognitive attributes have proven to be more accurate than those using only cognitive variables in predicting student success. The Student Attitudinal Success Instrument (SASI-I), a survey assessing nine specific noncognitive constructs, was developed based largely on existing, validated instruments. It was designed to collect data on affective (noncognitive) characteristics for incoming engineering students (a) that can be collected prior to the first year and (b) for which higher education institutions may have an influence during students’ first year of study. This chapter will focus on the psychometric analysis of this instrument. Three years of data from incoming first-year engineering students were collected and analyzed. This work was conducted toward investigating the following research questions: Do the scale scores of the instrument demonstrate evidence of reliability and validity, and what is the normative taxonomy of the scale scores of first-year engineering students across multiple years? Further, to what extent did the overall affective characteristics change over the first year of study?",book:{id:"11441",title:"Psychometrics - New Insights",coverURL:"https://cdn.intechopen.com/books/images_new/11441.jpg"},signatures:"Kenneth J. Reid"},{id:"82112",title:"Comparative Senescence and Lifespan",slug:"comparative-senescence-and-lifespan",totalDownloads:8,totalDimensionsCites:0,doi:"10.5772/intechopen.105137",abstract:"The word senescence is derived from the Latin word “senex” (meaning old). In biology, senescence is a process by which a cell ages and permanently stops dividing. Senescence is a natural universal phenomenon affecting all living organisms (e.g., humans, animals, and plants). It is the process of growing old (aging). The underlying mechanisms of senescence and aging at the cellular level are not fully understood. Senescence is a multifactorial process that can be induced by several stimuli including cellular stress, DNA damage, telomere shortening, and oncogene activation. The most popular theory to explain aging is the free radical theory. Senescence plays a role in the development of several age-related chronic diseases in humans (e.g., ischemic heart disease, osteoporosis, and cancer). Lifespan is a biological characteristic of every species. The lifespan of living organisms ranges from few hours (with mayfly) to potential eternity (with jellyfish and hydra). The maximum theoretical lifespan in humans is around 120 years. The lifespan in humans is influenced by multiple factors including genetic, epigenetic, lifestyle, environmental, metabolic, and endocrine factors. There are several ways to potentially extend the lifespan of humans and eventually surpass the maximum theoretical lifespan of 120 years. The tools that can be proposed include lifestyle, reduction of several life-threatening diseases and disabilities, hormonal replacement, antioxidants, autophagy inducers, senolytic drugs, stem cell therapy, and gene therapy.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Hassan M. Heshmati"},{id:"81748",title:"Emotional Creativity",slug:"emotional-creativity",totalDownloads:5,totalDimensionsCites:0,doi:"10.5772/intechopen.104544",abstract:"Creativity encompasses and is influenced by several emotions. Emotional creativity is a critical component in the creative process. It is the ability to create something new through the influence of emotions evoked from the personal or experiences of others. Creative works inspired by emotions are often original and greeted with Euphoria. This chapter demonstrates how different emotions inspire different forms and levels of creativity with examples of notable artists who experienced emotional creativity. This chapter discusses research linking emotions to creativity and the explanations of how the identification and regulation of emotions, which are often referred to as emotional intelligence, make a difference in whether creativity becomes useful, helpful, or hurtful. The dark side of creativity, which occurs when creativity becomes hurtful instead of helpful, and research into its causes is also discussed in this chapter. An example of a notable figure in the twentieth century, i.e., Adolf Hitler who masterminded World War II, is used to demonstrate how emotions played a role in this phenomenon. A conclusion alluding to the fact that creativity or creative outcomes are not necessarily bad but the application of creative work and the ability to identify emotions and regulate or control the emotions to drive creative performance validates emotional creativeness.",book:{id:"11303",title:"Creativity",coverURL:"https://cdn.intechopen.com/books/images_new/11303.jpg"},signatures:"Cynthia Naa Anyimah Botchway"},{id:"82097",title:"Including Religion in Rational-Emotive Behavior Counseling",slug:"including-religion-in-rational-emotive-behavior-counseling",totalDownloads:6,totalDimensionsCites:0,doi:"10.5772/intechopen.104980",abstract:"Cognitive-behavioral therapies (CBT) have been disseminated worldwide. This therapeutic approach is being considered some of the best empirically supported treatments for a large variety of psychological disorders. The core tenet of CBT is to restore mental health and promote psychological well-being by focusing on cognitive dysfunctional patterns that cause emotional distress and maladaptive behaviors. First, a general view of the basic principles and origins of cognitive-behavior therapies constitute the basis on which the chapter is built. Thereafter, a more in-depth discussion on specific forms of CBT, namely cognitive therapy (CT) and rational-emotive behavior therapy (REBT) provides further support for the integration of religion and psychological intervention. Next, a thorough analysis of the theoretical premises of this integration and the ways in which religious beliefs and psychological mechanisms merge in practice is provided. Finally, the REBT conceptualization, techniques, and strategies are illustrated in a practical situation; here, the relationship between religious beliefs and practices on the one hand, and irrational beliefs, dysfunctional emotions, and maladaptive behaviors, on the other hand, is easy to observe. The example provided aptly illustrates the many ways in which REBT can incorporate religious principles, beliefs, and practices; all of them, advocating for the harmonious relationship between Christian values and REBT.",book:{id:"11305",title:"Counseling and Therapy - Recent Developments in Theories and Concepts",coverURL:"https://cdn.intechopen.com/books/images_new/11305.jpg"},signatures:"Adrian Opre and Bianca Macavei"},{id:"82075",title:"Sexuality and Disability",slug:"sexuality-and-disability",totalDownloads:9,totalDimensionsCites:0,doi:"10.5772/intechopen.104325",abstract:"Sexuality and disability is an important topic in our global society. Dismantling myths about sexuality and disability is considered a final frontier for people with disabilities. Dismantling myths about sexuality and disability is vital to the overall health and well-being of people with disabilities. A major aspect of the dismantling process is to acknowledge that sexuality is a significant quality of life determinant for all human beings. This chapter provides information that will promote a healthier and more accurate view of Sexuality and Disability. Dismantling this last frontier involves providing the readership with relevant historical information; information about psychosocial factors and attitudes that influence sexuality; and information about ethical practice guidelines. Information pertaining to sexuality training, specific provider competencies and how select disabilities and chronic illness impact sexuality is also covered in the chapter.",book:{id:"11267",title:"Human Sexuality",coverURL:"https://cdn.intechopen.com/books/images_new/11267.jpg"},signatures:"Danita H. Stapleton, Sekeria V. Bossie, Angela L. Hall and Lovett O. Lowery"},{id:"81962",title:"Gender and Modern-Day Slavery: Aggression and Violence in the Context of a Nigerian Focus",slug:"gender-and-modern-day-slavery-aggression-and-violence-in-the-context-of-a-nigerian-focus",totalDownloads:15,totalDimensionsCites:0,doi:"10.5772/intechopen.104727",abstract:"This chapter explored the various forms of gender-based modern-day slavery in Nigeria. Such modern slaveries as baby factories (unlawful maternity and orphanages where children are sold and their mothers serve as ‘economic-slaves, sex-slaves, procreation-slaves, and money-ritual-slaves’), peonage (debt bondage), and early forced marriage were found to be common and on the increase in Nigeria and fundamentally precipitated by patriarchy. The predictors and risk factors of these slavery typologies were supported by the tenets of feminist and political economy theories, which formed the framework. A patriarchal society engenders inequalities, alienation, subjugation, aggression, violence, deprivations, and frustration, with women on the receiving end, when compared to men. The female population is largely the victim, while most perpetrators are males. The new, emerging trend in slavery enterprise are typically organised and largely motivated by women’s vulnerabilities and powerlessness, with dehumanising and destructive consequences. Arising from this is the conclusion that certain cultural practices and socioeconomic forces intertwine with poverty, lack of qualitative education, and other exclusions to expose women and girls to servitudes. The problem requires robust intersectoral approaches—that is, coordinated intervention, programmes, and collaborative efforts between governments and local authorities and institutions—to (re)solve.",book:{id:"11440",title:"Aggression and Violent Behaviour",coverURL:"https://cdn.intechopen.com/books/images_new/11440.jpg"},signatures:"Mary Juachi Eteng and Macpherson Uchenna Nnam"}],onlineFirstChaptersTotal:59},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. 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He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. 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He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. 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