Francisco Daniel Hernandez Castillo

By Francisco Daniel Hernández Castillo, Francisco Castillo Reyes, Gabriel Gallegos Morales, Raúl Rodríguez Herrera and Cristobal N. Aguilar

Part of the book: Research in Organic Farming

By Diana Jasso de Rodríguez, F. Daniel Hernández-Castillo, Susana Solís-Gaona, Raúl Rodríguez- García and Rosa M. Rodríguez-Jasso

Part of the book: Integrated Pest Management and Pest Control

By Hernández F.D. Castillo, Castillo F. Reyes, Gallegos G. Morales, Rodríguez R. Herrera and C. Aguilar

Part of the book: Weed and Pest Control

By Reynaldo De la Cruz-Quiroz, Sevastianos Roussos, Daniel Hernandez-Castillo, Raúl Rodríguez-Herrera, Lluvia Itzel López- López, Francisco Castillo and Cristóbal Noé Aguilar

The solid-state fermentation (SSF) is the best option to produce spores of biological control agents (BCA), because the spores have a long shelf life, compared with the obtained in liquid cultures. The spore production under SSF conditions using polyethylene bioreactors (bag-type) is a new topic. Only little information mainly about bioreactors design and adequate conditions to spore production is available. The main aim of this study was to use the corn cob as substrate in SSF and produce spores of the fungi BCA Trichoderma asperellum in a polyethylene bioreactor. In the process was added biomass of the phytopathogenic fungi Colletotrichum gloeosporioides and Phytophthora capsici as inducers of hydrolase enzymes (endoglucanases, exoglucanases and chitinases). It is possible to obtain high levels of spores, cellulases and chitinases using a polyethylene bioreactor under SSF conditions by T. asperellum and corn cob as substrate. Under the SSF conditions evaluated, the biomass of C. gloeosporioides has an inducer effect just on the spore production. However, P. capsici have effect on all response variables evaluated. The spore production was twice when used P. capsici as inducer. The most influential factor under SSF was the moisture. Levels of 66 and 50% of this factor increase the yield in all response variables evaluated (sporulation, cellulases and chitinases), C. gloeosporioides and P. capsici, respectively.

Part of the book: Fermentation Processes

By Francisco Daniel Hernández-Castillo, Francisco Castillo-Reyes, Marco Antonio Tucuch-Pérez and Roberto Arredondo-Valdes

This chapter will cover topics about the microbial antagonists Trichoderma spp. and Bacillus spp. from the perspective of use as potential biological control agents on plant diseases. Results obtained in the laboratory about from their isolation, microbial strain collections for both genera, taxonomic identification, antifungal activity in in vitro tests, obtained evaluation of the antifungal effect of secondary metabolites from microbial antagonists will be shown. Besides, results obtained from bioassays in the greenhouse and field are used as biopesticides in the control of diseases in fruit trees and vegetables and their effects on the promotion of plant growth and increased crop yield.

Part of the book: Organic Agriculture