Graziella Joanitti

Graziella Joanitti has experience in the fields of Cell Biology, Biophysics, Microbiology, Histology and Biotechnology as a researcher. She earned her Bachelor's degree in Biological Sciences in 2005 and Teacher degree in Biological Sciences in 2006 at the University of Brasilia (UnB), DF, Brazil. She completed her Master degree course in 2008 on the same institution, with the Project Cytostatic and citotoxic effects of a Bowman-Birk protease inhibitor from Vigna unguiculata seeds on breast cancer cells (MCF-7).(Advisor: Dr. Ricardo B. Azevedo). Currently, she is developing her PhD thesis under the title Preventive and therapeutic effects of a Bowman-Birk protease inhibitor entrapped in liposomes on breast and skin cancer, in vitro and in vivo.(University of Brasilia, Advisor: Dr. Ricardo B. Azevedo). Graziella has been working as a researcher from 2001-2010, working mainly with protease inhibitors and frog skin peptides as preventive and therapeutic anticancer agents; Cell death mechanisms induced on cancer cells; Extraction and purification of proteins from plant seeds; and Production and characterization of liposomes. During this period, she had the opportunity to work with several researchers of the Cell Biology, Molecular Biology and Genetic & Morphology Departments at the University of Brasilia, acquiring extensive work experience. Briefly, her laboratory experience comprises: microscopy (light, phase-contrast, confocal) and image analysis; Histology; Immunohistochemistry; ELISA; Cell viability assays; Sonication; Extrusion; Chromatography; Dialysis; Protein electrophoresis; Protein and lipid quantification; Enzimatic assays; Cell culture; Flow cytometry; Laboratory animal care; Animal models of cancer; and Chemical carcinogenesis induction in mice. Her experience as a teacher includes volunteer teaching in courses such as Basic Histology for biology, odontology and veterinary undergraduate students at UnB.

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