\r\n\t- Traditionally accepted topics related to global health security,
\r\n\t- The impact of human activities and climate change on “planetary health”,
\r\n\t- The impact of global demographic changes and the emergence chronic health conditions as international health security threats.
\r\n\t- A theme dedicated to the COVID-19 Pandemic,
\r\n\t- Novel considerations, including the impact of social media and more recent technological developments on international health security.
\r\n\tThe goal of this book cycle is to provide a comprehensive compendium that will be able to stand on its own as an authoritative source of information on international health security.
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Miller",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/10624.jpg",keywords:"Threats, Monitoring, Food Security, Emerging Infections, Transmission, Geopolitics, Climate Change, Cyber Health Security, COVID-19, Novel Coronavirus, Pandemic, Coronavirus",numberOfDownloads:167,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"August 20th 2020",dateEndSecondStepPublish:"November 5th 2020",dateEndThirdStepPublish:"January 4th 2021",dateEndFourthStepPublish:"March 25th 2021",dateEndFifthStepPublish:"May 24th 2021",remainingDaysToSecondStep:"2 months",secondStepPassed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"An Associate Professor of Surgery at Temple University School of Medicine and a Chair of the Department of Research and Innovation, St. Luke's University Health Network. A member of multiple editorial boards and co-author of over 550 publications.",coeditorOneBiosketch:"An Associate Professor of Surgery & Integrative Medicine at Northeast Ohio Medical University and Cardiothoracic Surgeon at the Summa Health Care System. A prolific writer and presenter, with multiple books, hundreds of peer-reviewed articles, and innumerable presentations around the world.",coeditorTwoBiosketch:"A CEO of the INDUSEM Health and Medicine Collaborative, Global Executive Director. of the American College of Academic International Medicine (ACAIM) and head of the World Academic Council of Emergency Medicine.",coeditorThreeBiosketch:"A Director of Research in the Department of Emergency Medicine at Nazareth Hospital in Philadelphia, USA, and co-chief editor of the International Journal of Critical Illness and Injury Science. 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His academic experience over the last two decades includes consistent achievements and innovative strategies that have led to the creation of organizations, publication of landmark papers, and commendation with prestigious citations and honors for his works that have impacted academic medicine globally. He has played a defining role in founding and building internationally recognized interdisciplinary indexed journals. He is the CEO of the INDUSEM Health and Medicine Collaborative and heads the World Academic Council of Emergency Medicine (WACEM). Additionally, he serves as the Global Executive Director for the American College of Academic International Medicine (ACAIM). 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He completed residencies in Emergency Medicine and Internal Medicine at the State University of New York (SUNY) Downstate Medical Center (2010) where he served as Chief Resident for Research. He completed fellowships in Pulmonary Medicine at the University of Pittsburgh Medical Center (2013) and Critical Care Medicine at the National Institutes of Health (2014). He is active in the American College of Academic International Medicine, and is co-chief editor of the International Journal of Critical Illness and Injury Science. 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It can have catastrophic outcome in an otherwise simple procedure, such as a regional dental anesthesia, local anesthesia, nerve block either peripheral, intravenous or peridural. The clinical phenomenon of LAST has been known for more than 100 years. Historically, the introduction of cocaine as the first local anesthetic (LA) in late nineteenth century was soon accompanied by reports of its systemic toxicity. The symptoms of systemic toxicity were frequently described as seizures or respiratory failure, but some cases also included reports of adverse cardiac effects [1]. These reasons demanded medical and pharmaceutical industries to search for new less toxic LA.
At first, manipulation of existing molecular structures after analyzing natural products lead to development of few drugs. It became clear that the development of more LA of ester group did not return the desired results. The main concern was short duration of action due to instability of ester bonds. The drugs were prepared in an oily formulation to increase the duration (which proved neurotoxic locally, or by enhancing the lipophilicity of the molecule which made it more toxic to central nervous system (CNS) as well as cardiovascular system (CVS)). Lidocaine, synthesized in 1944 was the first amide LA drug to be used clinically. It gave a dependable block, but unfortunately was short acting. In search of an LA with a longer duration of action than lidocaine, a family of N-alkylpiperidines 2,6 xylidides was introduced in 1950s. It was shown that increasing N-alkyl carbon chain length (to max at C4 or C5) increased lipophilicity, which increases duration of action, but unfortunately also increased systemic toxicity. Derivatives developed for clinical use included mepivacaine, bupivacaine, ropivacaine, levobupivacaine, etc. But in subsequent year’s cases of lethal LAST were documented.
Accidental intravascular injection during regional anesthesia is the most common cause of LAST. Some comorbidities may increase the risk of LAST; liver failure, heart disease, pregnancy and metabolic syndromes. In addition, patients at extreme ages have an increased risk factor of toxicity, due to the reduction in anesthetic clearance. Children under 4 months of age have low plasma concentrations of acid glycoprotein, which may result in a lower intrinsic clearance of bupivacaine [2, 3].
The incidence of LAST is extremely variable from zero events after more than 12,000 nerve blocks to 25 per 10,000 nerve blocks. One study reported seizures in 79 of 10,000 brachial plexus block procedures. This complication was due to toxicity of the CNS as a result of intravascular LA injection. Although the published data are inconsistent, the prevailing goal is to prevent LAST, and when it manifests, it must be treated quickly and effectively [4, 5, 6].
Cardiac toxicity is the most important component of LAST since, unlike the CNS toxicity, it can end in cardiac arrest and death. It is often due to an intravascular injection not noticed during nerve block. LA bind and inhibit voltage dependent sodium channels. It leads to conduction disorders, contractile dysfunction and ventricular arrhythmias. The incidence of cardiac toxicity increases with bupivacaine due to its affinity for inactive sodium channels during the cardiac action potential at a concentration of 0.2 μg/mL. This is done in a fast/slow manner, which means that bupivacaine binds very rapidly to a large proportion of sodium channels during the potential for cardiac action, but is slowly released from the channels during diastole, which results in a large proportion of medication that accumulates at 60–150 beats per minute. Lidocaine at 5–10 μg/mL will also result in a substantial blockage of sodium channels during a potential for cardiac action. However, in contrast to bupivacaine, lidocaine follows the principle of rapid entry/exit, which means that it is rapidly released from sodium channels during diastole. This allows faster recovery and a lower incidence of cardiac toxicity compared to bupivacaine.
CNS toxicity is another important consequence of LAST. Although it is composed of many initial prodromal features, it is most often manifested as seizures. A mechanistic theory focuses on acid-sensitive K+ channels. These pH-sensitive channels generate neuronal currents of leakage of potassium. LA inhibition causes membrane depolarization and increased neuronal excitability. As these channels are expressed throughout the brain, this is the suggested mechanism for seizures in this context. Consequently, it became mandatory to understand the mechanism of LAST, so that it could be prevented and managed efficiently.
This chapter reviews the mechanisms, frequency, clinical characteristics, prevention and treatment of LAST.
LA agents are classic sodium channel inhibitors. LAST hypotheses are based primarily on the binding site, ion channels, signaling pathway or enzymes involved in the CNS and cardiac toxicity or its treatment. LA inhibit some components of the oxidative phosphorylation pathway, which affects the myocardium and the CNS that are poorly tolerant of anaerobic metabolism. These deleterious effects differ quantitatively between LA, doses and administration routes. It is appropriate to first review the ion channels implicated in LAST [7, 8, 9].
LA are sodium channel blocking acting on inactivated sodium channel state and blocking it. They affect the initial depolarization phase and slow it down, leading to slow cardiac conduction. The antiarrhythmic property of lidocaine is related to this slowed conduction as it causes fast blockade of the channels. In contrast, bupivacaine and ropivacaine cannot be used as anti-arrhythmic drugs though they have fast blockade, but slow release of sodium channel (lasting longer than 1 s in contrast to lidocaine which lasts 0.15 s) [10]. Slowed conduction leads to widening the QRS complex, prolongation of the PR interval, AV block and, eventually, ventricular fibrillation due to the unidirectional blockade and re-entry phenomenon.
Out of three known organized potassium channels, two groups are of interest where LA toxicity is concerned. Out of these, one group of channels are known as the inward, outward and transient rectifier potassium channels, thus plays an important role in the potassium efflux during phases 2 and 3 of the cardiac muscle action potential [11]. If these channels are blocked, it will lead to prolongation of action potential; i.e. phase 2, delay in repolarization; i.e. phase and shift the resting membrane potential more positive (phase 4) to increase automaticity [12]. The second group of potassium channels of interest are K2p. Previously known as the delayed rectifier channels, these channels are believed to be responsible for the background or leak potassium currents. In this setting, they control the resting membrane potential. A blockade of these channels shifts the resting membrane potential towards spontaneous depolarization. K2p channels are extensively spread in the body. In the CNS they are mainly located in the thalamo-cortical and striatal neurons, where blockade leads to increased neuroexitability [13]. They are also present in high concentrations in the cerebral blood vessels, where blockade leads to vasoconstriction and decreased cerebral blood flow. K2p channels are also present in neurons of the auditory system, where blockade leads to tinnitus. LA agents are also known to have K2p mediated stimulating effect on ventilation [14]. They are located in the brainstem and carotid body, where they regulate the respiratory response to carbon dioxide via sensing the changes of pH and expressed in the oxygen-sensing cells of the glomus body respectively. K2p channels are sensitive to changes in oxygen tension and extracellular pH and are potentiated by volatile anesthetic [13]. In the CVS K2p channel blockade predisposes the patient to re-entry dysrhythmias. It is well known that hyperkalemia exacerbates LAST, and that K+ATP openers (which effectively lowers intracellular K+ levels) attenuate the toxic effects of bupivacaine [15].
All voltage-gated Ca2+ channels are comprised of two subunits according to the latest research: α and β subunit. The α subunit has fairly constant chemical structure for all voltage gated Ca2+ channels and is the main pore- forming element of the channel. The β subunit has highly variable structure that depends on the location and function of the channel for e.g. in cardiac conduction tissue β1 subunit completes the ion channel structure. The role of the β1 subunit seems to be the modulation of channel opening and membrane ion trafficking [16]. In terms of their physiological effect, the heart has two distinct types of channels namely the T-type (transient) that are low voltage activated channels (LVA), and L-type (long lasting). On the other hand, are high voltage activated channels (HVA). The T-type channels are mainly located in the pacemaker cells of the sinoatrial node, and the opening of these channels completes the prepotential required for the pacemaker potential. L-type channels are present on the surface of the myocytes of both atrium and ventricle, and are closely associated with the T-tubules. The plateau phase (phase 2) of cardiac muscle action potential is produced by opening of these L-type channels. LA bind to these channels and predispose them to an inactivated state. The consequence of this is prolongation of the action potential (phase 2) and depressed contractility [9].
Risk factors to develop LA toxicity are related to kind of LA used, the type of nerve block, and the patient.
The most important and most studied factors in the development of LAST are undoubtedly the type and dose of LA.
The kind of LA injection influence toxicity risk. Animal studies showed that [17] more levobupivacaine than bupivacaine was required to induce cardiac arrest and levobupivacaine caused fewer convulsions and arrhythmias than bupivacaine, at similar doses [18]. Ropivacaine may cause less motor block, but whether it is clinically significantly less toxic is unknown. Other property differentiating the toxicity of LA is their intrinsic effect on vessels, where levobupivacaine and ropivacaine have intrinsic vasoconstrictors properties (that may prolong duration of action and slow systemic absorption), whereas bupivacaine is an intrinsic vasodilator. The clinical significance of this difference remains unclear. Another important concept in the study of LAST is the ratio of the dose required to produce cardiovascular collapse to that required to induce seizures, the so called CC/CNS ratio (ratio of dose causing cardiovascular collapse to the dose causing seizures). Bupivacaine has a CC/CNS ratio of 2.0 compared with 7.1 for lidocaine. Therefore, progression from CNS signs and symptoms to cardiovascular collapse can occur more readily with bupivacaine than with lidocaine.
Determining the optimal dose of LA to use is complex and always a topic of debate. Using the lowest effective dose is always prudent and advisable practice along with consideration of patient characteristics and site of administration. Some may argue that the recommended doses provide a rough guide for clinical use. The maximum weight-based doses have lost rationale in others view as such dosing does not correlate to the resulting blood level and does not take into account relevant patient factors or the site of injection. Other factors that question the maximum weight based doses are variation between different texts and countries, no recommendation whether dose calculation is based on actual body weight or ideal body weight and such hard and fast dosing rules do not take into account the complete clinical context [19]. As a result, if dosing is calculated on actual body weight, the obese, pregnant, or both patients may receive a dangerously high dose.
Inadvertent intravenous injection of LA can occur during any regional anesthetic technique, but tends in becoming LAST only if larger volumes (5–10 mL) are injected, or small doses during face and neck procedures. Symptoms usually occur within 3–5 min and can be severe if appropriate measures are not taken. LAST resulting from the gradual systemic absorption of LA is characterized by a late presentation (20–30 min or more after a bolus injection), and usually occurs when a relatively high dose of LA has been administered in the presence of another risk factor. Local anesthetic systemic toxicity may also occur in the context of continuous LA infusion, in which case the onset may be hours to days after starting the infusion. The symptoms last until the drug metabolism reduces concentrations plasma levels below the toxic threshold. Prolonged monitoring and supportive therapy are essential.
Blocking site is important since rate of absorption, chances of direct intravascular injections and thus chances of toxicity depends on the anatomical location. For example, interscalene block, stellate ganglion block, intercostal nerve block have a higher risk of direct intravascular injection and other blocks like scalp, bronchial mucosa, inter pleural cavity carry an increased risk of rapid absorption and toxicity due to the injection being in a highly vascularized area. The classic order of sites propensities to lead to toxicity, in order from lowest to highest: subcutaneous injection, brachial plexus, epidural, caudal, and finally intercostal blocks and topical mucosal anesthesia.
Performing the block in a safer way decreases the chances of toxicity by manifolds. The practice of giving the dose of LA in incremental injections, after frequent aspiration, adding test dose and most important is using ultrasound-guided needle placement to give the LA reduces the risk of toxicity.
Various factors are related to toxicity of LA. Most common being the free peak plasma concentration, perfusion at the site of injection, co-morbidities (renal, liver, metabolic, and cardiac diseases). As already discussed earlier, more the perfusion at the site of injection, more will be the peak plasma concentration as systemic absorption is accelerated. A low α1-acid glycoprotein (AAG) titer results in a higher concentration of free LA.
In patients with severe renal impairment there may be slightly increased risk of toxicity as these patients typically have a reduced clearance of LA, hyper dynamic circulation but increased AAG. So, it is prudent to reduce the initial dose by 10–20% according to severity of renal impairment.
In patients with liver disease, single dose blocks are unaffected, but the doses for repeat boluses and continuous infusions should be reduced. Such patients may also have renal or cardiac disease. AAG is synthesized in patients with end-stage liver disease, offering some protection against LAST. Patients with severe cardiac failure are particularly susceptible to LA-induced myocardial depression and arrhythmias. Further, lower liver and renal perfusion slows metabolism and elimination, so safe initial and maintenance doses of LA are correspondingly lower too. On the other hand, poor perfusion at the injection site may decrease peak plasma concentrations.
Extremes of age have different physiological changes making them different from young and adolescent age group. In elderly patients; there is a safety benefit in dose reduction without altering the clinical efficacy. In the geriatric patient’s nerves appears to be more sensitive to LA due to various factors like altered nerve morphology, there is less fat tissue surrounding the nerves, and axonal function is also reduced. Moreover, this age group has multiple co-morbidities and decreased muscle mass; blood flow to organs is reduced, decreased clearance and organ function. In patients older than 65 years’ involuntary overdoses being responsible for some cases of LAST. When considering the use of LA in geriatric patients, it is mandatory to pay special attention to the presence of systemic disease and muscle wasting [20].
In neonates and infants, the risk of accumulation of LA with continuous infusions is more than in adults as AAG levels are reduced (about half that of adult at birth). Children have an increased elimination half-life of LA, which in neonates is increased to 2–3 times that of an adult. Bupivacaine accumulates with continuous infusion and 2-chloroprocaine can be used as an alternative. LAST has the highest incidence in infants less than 6 months of age and is associated with bolus dosing and penile nerve blocks [21].
Pregnancy is one of several clinical settings in which LAST can be potentiated. Pregnant patients are at an increased risk of toxicity as they have increased perfusion as well as decreased AAG levels; thus high peak free plasma concentration of LA. Some of the earliest anecdotal reports of LAST-related fatal cardiac arrests involved pregnant women [1]. Notably, several of the original anecdotal reports of LAST-related fatal cardiac arrests involved pregnant women. It has since been proven that pregnancy increases the risk for LAST, and subsequent guidelines preclude use of 0.75% bupivacaine in late gestation because this concentration was involved in cases of fatal toxicity in parturients [22, 23, 24].
Most of the LAST events happen a few minutes after the LA injection and present with signs of the CNS, which may or may not be accompanied by changes in the CVS (Figure 1). Sometimes, the manifestations are atypical in terms of time and clinical picture. The onset of symptoms may be delayed up to 60 min after a bolus injection, and signs of CVS toxicity may appear in the absence of any CNS characteristics. This last scenario may be more common in patients who are very sedated or under general anesthesia. High plasma concentrations during LAST can occur in three circumstances: inadvertent intra-arterial injection, intravenous injection or systemic absorption, each of which has a characteristic temporal course. LAST associated with intra-arterial injection occurs classically during nerve blockages in the head and neck (stellate ganglion, interscalene or deep cervical plexus blocks) in which there is an involuntary injection of LA in an artery that supplies the brain. The symptoms of the CNS, usually seizures, occur almost immediately. When the injected dose is small, progression to CVS collapse is uncommon. Recovery is equally rapid since LA quickly redistributes from the cerebral circulation [25]. Figure 2 shows an intra-arterial puncture detected during the blockage of the stellate ganglion in a patient with herpes zoster neuropathy.
Scheme showing the most important clinical manifestations of LAST.
Accidental arterial puncture during a stellate ganglion block in a patient with postherpetic neuropathy.
Central nervous system toxicity could be presumed as a two-stage process in which initial blockade of Na+ channels occurs in the inhibitory neurons thus allowing excitatory neurons to act unopposed which culminates in generalized convulsions. Higher concentrations of LA agent affect all neurons, leading to global CNS depression, which is clinically seen as coma and evident on EEG as slowing and ultimately silent EEG. In most cases, convulsions, although an impressive clinical entity, can be handled safely without permanent brain damage. Clinical manifestations of the systemic neurotoxicity of LA usually occur in stages; in the initial phase there is perioral numbness, confusion, tinnitus followed by an exciting phase that shows seizures and, finally, a depressive phase where there is loss of consciousness and respiratory depression.
Both the direct and indirect effects of the LA drugs on myocardium are involved in the mechanism of cardiovascular toxicity [26].
Clinical presentation includes hypertension and tachycardia in initial phase (during CNS excitatory phase), followed by intermediate phase showing myocardial depression, decreased cardiac output and thus hypotension, followed by sinus bradycardia, peripheral vasodilatation, conduction defects and dysrhythmias in terminal phase.
All medical and paramedical personnel working in operating theaters and hospital locations where LA are used should be prepared to recognize, diagnose and treat patients with signs and symptoms of LAST in a timely manner. The ASRA has published and updated the guidelines recommended by experts for the management of LAST (Table 1) [4, 5]. The initial treatment of LAST should focus on keeping the airways permeable with adequate ventilation, circulatory support and the reduction of systemic side effects. Immediate ventilation and oxygenation to prevent hypoxia and acidosis can facilitate resuscitation and reduce the likelihood of progression to seizures or cardiovascular collapse [24, 25].
Checklist for LAST treatment | |
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The pharmacologic treatment of LAST is different from other cardiac arrest scenarios | |
Reduce individual epinephrine boluses to ≤1 mcg/kg | |
Avoid vasopressin, calcium channel blockers, beta blockers, or other local anesthetics | |
| |
Lipid emulsion 20% (precise volume and flow rate are crucial) | |
Greater than 70 kg patient | Less than 70 kg patient |
Bolus 100 mL lipid emulsion 20% rapidly over 2–3 min | Bolus 1.5 mL/kg lipid emulsion 20% rapidly over 2–3 min |
|
|
If patient remains unstable: | |
|
All patients receiving LA injections in doses potentially to trigger LAST should have oxygen, standard monitoring which is to be continued at least 30 min after completion of injection to detect delayed presentation if any, and intravenous access applied [27, 28]. Immediate access to LAST Management Checklist is advisable, and all medications and resuscitation equipment required should be immediately available, preferably in the form of a “LAST Rescue Kit”.
Immediate management involves the general safety and resuscitation measures that are essential in any emergency. First, stop LA injection and call for help. The immediate priority is to manage the airway, breathing, and circulation. Avoid factors potentiating LAST like hypoxia, hypercarbia, and acidosis (metabolic or respiratory [24, 25, 29]).
Use of intravenous lipid emulsion as a therapeutic modality comes with several advantages. Theories suggest that it improves cardiac conduction, contractility and coronary perfusion by removing the liposoluble LA from cardiac tissue. Better understanding of the mechanism of action of lipid emulsion with recent advances underlines its importance as therapeutic modality in the management of LAST. First advantage is that lipid emulsion may shuttle any LA agent from high blood flow organs to detoxification organs such as the liver [30]. Secondly, lipid emulsion therapy may also improve the cardiac output and blood pressure. Post conditioning myocardial protection may also occur [31, 32, 33, 34]. There is an unavailability of large scale data collection and other prospective studies to demonstrate efficacy of lipid emulsion therapy due to various difficulties [24, 35, 36]; however various animal studies provide strong support for use of lipid emulsion therapy to reduce mortality when used along with resuscitative measures [37]. Early administration of 20% intravenous lipid emulsion therapy should, therefore, be an immediate priority after airway management in any LAST event that is judged to be potentially serious. A bolus of 1.5 mL/kg of 20% lipid emulsion and the subsequent infusion of 0.25 mL/kg per minute should be administered. The infusion should be continued for 10 min after reaching hemodynamic stability. An additional bolus and an infusion rate increase to 0.5 mL/kg per minute can be administered if stability is not achieved. The maximum recommended dose for initial administration is approximately 10 mL/kg for 30 min (Table 1) [4, 37, 38]. Lipid emulsion remains first-line therapy (in conjunction with standard resuscitative measures) in LAST. However, more research in humans is necessary to establish its real usefulness [36].
Prompt seizure prevention and termination is crucial to avoid injury and acidosis. Benzodiazepines are first line therapy for management of seizures due to their cardio stable profile. Propofol or thiopental can be used in low doses, although these drugs may worsen the hypotension or cardiac depression associated with LAST. It should be avoided in patients with cardiovascular compromise. Neuromuscular blockade can be considered in cases of ongoing seizures; small doses of succinylcholine should be administered intermittently to stop muscle activity and increased acidosis. Early termination of seizure activity helps to avoid metabolic acidosis and hypoxia that occurs due to repeated muscular contractions [24].
The treatment of LA-induced cardiac arrest focuses on restoring cardiac output, to restore tissue perfusion, prevent and treat underlying acidosis. Chest compressions should be started without delay and to be continued till return of spontaneous circulation (as per ACLS algorithms for cardiopulmonary resuscitation). If epinephrine is used, small initial doses of ≤1 μg/kg are preferred to avoid impaired pulmonary gas exchange and increased afterload [39]. Vasopressin is not recommended, as it can cause pulmonary hemorrhage. If myocardial LA levels is more than the threshold that corresponds to ion channel blocking concentrations, then the ionotropic effect of lipid emulsion therapy remains questionable. Chest compressions ensure the coronary perfusion that is sufficient to reduce tissue LA levels. In the absence of rapid recovery following ACLS measures and intravenous lipid emulsion therapy, early consideration should be given to cardiopulmonary bypass for circulatory support. For other deleterious CVS effects—such as arrhythmias, conduction block, progressive hypotension, and bradycardia – standard ACLS algorithms should be followed with the omission of LA, such as lidocaine and procainamide. Amiodarone is the first-line antiarrhythmic in the event of ventricular dysrhythmia. In addition, calcium channel blockers and B-adrenergic receptor blockers are not recommended.
Report the case of LAST to the registry at
The primary objective of every anesthesiologist is always patient safety. Pre-anesthetic evaluation, patient preparation, complete monitoring before starting administration of LA, and continuing this monitoring during surgery and the immediate postoperative period are mandatory.
During preoperative assessment, evaluate the patient for co-morbidities, evaluation of the risks and benefits of regional anesthesia for that individual should be discussed, patient should be explained regarding procedure and appropriate consent to be obtained.
Preparation includes selection of type of LA, prior calculation of doses, labeling all syringes.
All monitoring facilities to be available along with LAST rescue kit and resuscitative measures.
Block to be performed with continuous monitoring attached, with a capable help nearby.
The method of administration of LA should include the administration of incremental doses with frequent aspiration, ultrasound-guided needle placement, administration of test doses. This can help in the early detection of accidental intravascular placement of the needle or catheter and thus avoid erroneous administration of the LA, avoiding toxic plasma concentrations.
Continuous communication with the patient to detect early signs of intravascular injection like perioral numbness, tinnitus etc.
Clearly label any kind of spinal/epidural catheter or peripheral nerve block catheter; it should be documented well in patient chart also. Drug and dose already administered or to be administered should be well informed in instructions to the medical staff responsible for post-operative care of the patient. Continuous monitoring of vital signs in the postoperative period in suspected case of delayed LAST to be considered.
LAST is a serious life-threatening emergency, with protean manifestations, that can happen after administering LA. Anesthesiologists must understand its risks, prevention, and safe management. Promptly recognition and timely management of LAST can dramatically change the clinical course. In addition to the usual advanced cardiac resuscitation maneuvers, the current treatment focuses on the administration of lipid emulsion. While the development of new treatment plans can help limiting the associated morbidity and mortality, prevention remains vitally important. It is mandatory for all the practitioners using LA to understand patho-physiological basis, mechanisms, risk factors, prevention and treatment modalities of LAST.
HIV-1 was first identified in the 1980s and currently infects ~37 million people world-wide [1]. There has been a concerted global effort in strategic planning for HIV prevention, with the current goals in multiple countries for the end of 2020 being to significantly reduce or virtually eliminate new infections. This goal is based on the increased uptake and availability of anti-retroviral therapy (ART) and the more recent pre-exposure prophylaxis (PrEP), which has been embraced by many countries, particularly the US, UK and Australia [2], and demonstrated in landmark studies to prevent transmission by 99% [3, 4]. Additionally, the recent OPPOSITES ATTRACT study [5, 6] and PARTNER study [7] both showed that ART effectively reduces that rate of transmission to zero in homosexual male serodiscordant couples.
\nHowever, while ART is highly effectively at controlling HIV-1 infection, it does not impact the latent reservoir, which is established early during virus infection. This allows the latent virus to recrudesce following any treatment interruption and results in rapid virus rebound to pre-therapy levels. Therefore, ART does not constitute an HIV-1 cure. As is the case for many other diseases, gene therapy is being explored as an alternate HIV-1 treatment. There are, however, challenges specific to HIV, that may not arise in gene therapy approaches for other diseases. In the context of HIV, the latent reservoir represents a major barrier for developing an HIV cure, since ART has no effect on the integrated provirus and there is currently lack of a biomarker to identify cells that carry latent virus. Combined with the inability to identify latent cells and the rare frequency of latently-infected cells (~1 in 106 cells) these hurdles make the development of a gene delivery platform uniquely challenging, especially in terms of a systemic in vivo approach. Another difficulty is that not all integrated provirus is the same, with some being full-length intact genomes and others being defective genomes that carry large deletions and will not result in productive infection following reactivation [8]. These are the barriers that must be addressed to ensure a gene therapy approach to HIV translates into patient outcomes. The unique challenges facing HIV-1 gene therapy and current solutions are described in this chapter.
\nHIV-1 can infect a wide range of cells, predominantly targeting CD4+ T cells, dendritic cells, macrophages and other myeloid lineage cells [9]. This is achieved by binding of the viral envelope glycoprotein gp120 to the CD4 receptor, triggering a conformational change that allows for CCR5 or CXCR4 coreceptor binding. Further conformational changes in gp41 initiate a membrane fusion reaction that allows the viral capsid cytoplasmic entry [10]. Upon entry, the capsid disassembles to release viral RNA and proteins. To protect this genetic material, host restriction factors are subverted. With the aid of commandeered host cell machinery, a reverse transcription complex is formed and complementary viral DNA (cDNA) generated. The resulting pre-integration complex is transported via cytoskeletal manipulation to a nuclear pore complex where it is actively imported. The ability to traverse the nuclear membrane allows HIV to productively infect non-dividing cells. Viral integrase then facilitates the integration of viral DNA into the host genome to form the provirus [11]. Thus, HIV tethers its survival to the longevity of the cell and establishes the latent provirus reservoir, from which virus can reactivate. Using host replication machinery, viral RNA is then transcribed and exported to the cytoplasm. Proteins required for infectivity are synthesised and trafficked to the plasma membrane. Along with two RNA copies of the HIV genome, these proteins are assembled and packaged into immature virions in a process mediated by the viral Gag polyprotein. Once released from the plasma membrane, viral protease cleaves Gag into three structural proteins to create the mature infectious virion [12]. The HIV-1 life cycle is summarised in Figure 1.
\nHIV-1 life cycle and stages targeted by antiretroviral therapy (ART). ART drugs target various stages of the HIV life cycle, with some common drugs shown. Credit: National Institute of Allergy and Infectious Diseases (NIAID).
While the above cycle is the most common model of HIV-1 infection, it is not exclusive. Numerous alternate pathways at varying stages of infection have been observed. For example, rather than the release of free HIV-1 virions, cell-to-cell transmission via infectious synapses can occur [13]. This modification is a more efficient means of infectivity [14]. Additionally, the above model does not account for infection of the central nervous system [15], in particular cells such as astrocytes, which lack the CD4 receptor [16]. While some of the proposed pathways remain controversial, HIV-1 is undeniably a versatile virus capable of hijacking diverse systems. The infectious route it takes may depend on the cell type, its available resources and activation status.
\nDue to the inherent sequence variability of HIV-1 and the ability for virus resistance to arise, multiple stages in the virus life cycle need to be targeted by ART to control the infection. As illustrated in Figure 1, stages that are targeted can include binding, fusion, reverse transcriptions and integration, among others, with either one or more ART drugs from each stage being utilised to provide sufficient HIV-1 control. An example of one such drug combination is TRUVADA®, which combines two drugs targeting the reverse transcription stage, emtricitabine and tenofovir and has been widely embraced for PrEP treatment.
\nAlthough the versatility of HIV-1 presents a challenge for the development of therapeutics, by far, the latent cellular reservoirs are the greatest barrier to developing a cure. The difficulties in controlling these virus reservoirs arise when the infection becomes latent. While the exact mechanisms of HIV latency are still being precisely defined, studies have demonstrated epigenetic regulation is involved in suppressing virus transcription, with the presence of classic epigenetic repressive marks, including methylation (i.e. histone 3 lysine 27 trimethylation (H3K27me3)) and deacetylation (i.e. Histone deacetylase 1 (HDAC1)) on N-terminal histone tails inducing specific epigenetic chromatin compaction, termed heterochromatin [17].
\nThe latent reservoir resides in resting memory CD4+ T cells [18, 19], such as central memory, effector memory and transitional memory cells [8], T follicular helper cells [20], macrophages and other myeloid cells, as well as in immune privileged sanctuary sites, such as the gut [21], lymph nodes and associated germinal centers and the brain. Physiologically, in their resting states CD4+ T cells have low endocytic and metabolic rates that are sufficient for maintenance of housekeeping functions [22]. As such, they are not impacted by ART. To retain dormancy, they negatively regulate gene activation via inhibition of cellular transcription factors, such as NF-κB and NFAT [23]: host factors essential for initiating active HIV-1 virus production [24, 25, 26]. Consequently, the integrated provirus is reversibly silenced by epigenetic repression and evades host immune detection. This presents a clinically daunting prospect. As memory cells are long lived, with ART controlling the infection, not only would eradication of the reservoir take over 70 years [18], but theoretically, one infected cell could sufficiently sustain a life-long infection. Further, upon reactivation of the infected cell/s by any stimuli, virus recrudescence rapidly occurs and thus while ART can effectively control HIV-1 infection, it does not represent a cure.
\nVarious approaches to overcome the viral reservoir barrier are being pursued. Primarily, they can be separated into two main categories: sterilising and functional. Both approaches aim for an undetectable viral load without the need for ART, with the sterilising approach being defined as complete eradication of the virus, and conversely, the functional approach is defined as controlling the virus reservoir without its eradication.
\nThe most studied sterilising approach is aptly named “shock and kill”. This concept explores the use of latency reversing agents (LRAs) to “shock” the virus into reactivation, whereby it is detected and “killed” by either its own cytotoxicity or the host immune system. To date, this purging strategy has been largely unsuccessful. Proposed agents either induce global immune reactivation, leading to increased pro-inflammatory cytokines and severe side effects, or in vitro efficacy has simply failed to translate in vivo [27, 28, 29, 30, 31, 32]. Extensive descriptions of LRAs and the next step of optimising the “kill” step is reviewed by Kim et al., [33] and are not the focus of this chapter. However, one recent study has demonstrated a specific kill response mediated by ricin A, which is initially encapsulated in a novel nanocapsule polymer shell and then activated and released via the ricin A peptide crosslinkers being cleaved by HIV-1 protease [34]. Some of the LRAs under investigation are summarised in Table 1 and a schematic of the process is shown in Figure 2.
\nLatency reversing agents (LRAs) | \n|
Class | \nAgent | \n
Epigenetic Modifiers | \nHistone deacetylase inhibitors (HDACs) | \n
\n | Methylation inhibitors | \n
\n | Methyl-transferase inhibitors | \n
\n | Bromodomain inhibitors | \n
\n | P-TEFb activators | \n
Protein Kinase C Agonists | \nProstratin/Bryostatin | \n
\n | Ingenol B/PEP0005 | \n
PI3K/Akt pathway | \nDisulfiram | \n
\n | STATS signalling benzotriazole | \n
\n | mTOR complex Rapamycin | \n
TCR Activators | \nImmune checkpoint blockers | \n
Cytokines | \nIL-15 | \n
TLR Agonists | \nTLR7 | \n
\n | TLR9 | \n
Latency inducing agents (LIAs) | \n|
Class | \nAgent | \n
Epigenetic Modifiers | \nDidehydro-cortistatin A (dCA) | \n
\n | siRNA | \n
\n | shRNA | \n
\n | Ubiquitin-like, containing PHD and RING finger domains 1 protein (UHRF1) | \n
\n | Nullbasic | \n
HIV Integrase inhibitors | \nLEDGINs | \n
Agents that modulate HIV latency.
P-TEFb, positive transcription elongation factor b; TLR, Toll-like receptor; mTOR, mechanistic target of rapamycin; STAT5, signal transducer and activator of transcription 5; IL-15, interleukin-15; dCA, Didehydro-cortistatin A; UHRF1, Ubiquitin-like, containing PHD and RING finger domains 1 protein.
“Shock and kill” versus “Block and Lock” HIV cure strategies. Latency reversing agents (LRAs) activate or “shock” the HIV-1 provirus awake with the aim that the infected cell will then undergo “kill” via cytotoxic or host immune mechanisms. Latency inducing agents (LIAs) mediate the opposite effect, by “blocking” virus replication and “locking” the virus genome in an induced “super-latency” that is refractory to reactivation. HDAC, histone deacetylase; HMT, histone methyl transferase; PKC, protein kinase C; dCA, didehydro-cortistatin A; ART, antiretroviral therapy.
The limited success of sterilising cures has shifted the priority to identifying a functional cure; which is now seen as a more realistic approach to controlling the viral reservoir, without ART. The functional cure approach is termed “block and lock”. This concept exploits the use of latency inducing agents (LIAs) to “block” virus gene transcription at the promoter via epigenetic mechanisms and “lock” the integrated virus genome in a permanent super-latent state, which resembles the natural latent reservoir. We and others are pursuing this approach to provide a sustained virus remission, without ART. Some of the LIAs under investigation are summarised in Table 1 and a schematic of the process is shown in Figure 2.
\nIn 2012, the Valente group reported a novel small molecule inhibitor of HIV transcription, termed Didehydro-cortistatin A (dCA) [35]. Derived from a marine sponge, dCA inhibits Tat-mediated transactivation of integrated HIV provirus via disrupting binding of the transactivation response (TAR) element to Tat through direct binding competition with the RNA hairpin TAR-binding domain of Tat. Effectively disrupting the Tat/TAR complex, dCA induced prolonged suppression of virus transcription in HeLa-CD4 cells infected with HIV-1NL4.3 for 2 months with constant treatment and out to a further 27 days following cessation of treatment, as determined by measuring viral RNA levels. The inhibitory effect of dCA on HIV replication ex vivo in primary CD4+ T cells isolated from eight HIV-1 infected individuals on suppressive ART was ~60% compared to no treatment, while ART treatment alone reduced viral production to ~40% and dCA treatment alone reduced virus production to ~25% [35]. The study reported there were no apparent cytotoxic effects in cell culture models or when assessed in C57BI-6 mice at the concentrations used. Further optimisation of dCA will be required if the treatment is to be effective in the absence of ART, as desired for a functional HIV cure, and to increase the longevity of the suppressive effect.
\nRNA interference (RNAi) is a fundamentally conserved process crucial for viral defence and the regulation of normal gene expression. Since its initial discovery in transgenic tobacco plants [36], the RNAi field has erupted with literature exploring the depth of its possibilities. From a tool to study basic gene functions, to a remedy for previously untreatable conditions, RNAi has the potential to revolutionise research and medicine. As a result, it has been extensively studied and characterised in a wide array of organisms, particularly plants (Arabidopsis thaliana [37, 38]), the nematode Caenorhabditis elegans [39] and fission yeast Schizosaccharomyces pombe (S. pombe) [40]. Two distinct pathways have since emerged: post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS); also termed epigenetic silencing (refer to Figure 3 for an overview).
\nGene silencing pathways mediated by siRNA. siRNA duplexes induce posttranscriptional gene silencing (PTGS) predominately in the cytoplasm through RISC machinery initiating mRNA degradation, and transcriptional gene silencing (TGS) in the nucleus through the RITS complex inducing chromatin compaction to silence gene expression. Ago1, Argonaute 1; Ago2, Argonaute 2; shRNA, short hairpin RNA; RISC, RNA induced silencing complex; RITS, RNA-induced transcriptional silencing complex; TRBP, trans-activating response RNA-binding protein; TNRC6, trinucleotide repeat containing six proteins.
PTGS was the first distinct pathway to be discovered in 1998 by Fire and Mello [39]. It can be considered the predominantly cytoplasmic arm of RNAi. Briefly, short interfering RNAs (siRNAs) 19–23 base pairs long are processed by the Dicer endonuclease and loaded onto the Argonaute 2 (Ago2) protein. Several of other proteins are recruited to form the RNA-induced silencing complex (RISC). This complex uses the siRNA as complementary anti-sense guides to identify target mRNA in the cytoplasm that are subsequently cleaved and degraded by the catalytic activity of Ago2 [41]. As a result, target gene expression is transiently downregulated prior to translation. Exhibiting enormous potential, PTGS has since been developed as a tool to selectively inhibit the expression of critical HIV-1 viral proteins in vitro, leading to significant reductions in viral replication [42, 43, 44, 45, 46]. The sequence specific nature of the PTGS process suggests there are minimal off-target effects [47], although these can still occur. Some reports have shown that introducing exogenous siRNA less than 24 base pairs in length does not trigger an interferon mediated immune response or defence mechanism to the synthetic material [48].
\nWhile these are all highly desirable traits for future gene therapeutics, PTGS has several limitations, particularly in the context of HIV-1, which leave it unsuitable for gene therapy. A consequence of its high specificity is that a nucleotide mismatches between the siRNA and its target could be sufficient to abrogate silencing [49]. Hence, in the context of a highly diverse virus with multiple subtypes, a conserved target sequence must be carefully selected. Due to the enormous diversity of the genome, a multiplexed approach would be necessary to provide adequate coverage across HIV subtypes and strains. Additionally, as its silencing effects are only transient, PTGS would require either (1) frequent siRNA administration to an infected individual, thus rendering it a recurring treatment and not a cure, or (2) provision of a self-sustaining system. This would involve viral vector delivery of a plasmid that can constitutively express target siRNAs. This may be in the form of short hairpin (sh)RNAs that are exported to the cytoplasm and processed into the desired siRNA. The greatest pitfall however, is that PTGS predominately functions in the cytoplasm, targeting mRNA from an actively transcribing provirus. As such, it cannot overcome the selective pressures driving HIV-1 mutation. Rather, this process allows the virus to rapidly transcribe escape variants [50]. From point mutations and deletions that disrupt target specificity, to the generation of alternative secondary structures to prevent RISC accessibility [51], HIV-1 can rapidly circumvent a single PTGS therapeutic. Similar to ART, current PTGS therapeutics are having to be combined, to simultaneously target multiple HIV proteins and/or to target host targets, such as CCR5, to overcome the generation of resistance mutations [52].
\nIn comparison, TGS can be considered the nucleic arm of RNAi. It offers the highly specific targeting of the integrated HIV provirus. Still controversial in mammals, this pathway begins in the cytoplasm where siRNA associate with Argonaute 1 protein (Ago1). These two components are trafficked to the nucleus [53] and recruit other proteins to form the RNA-induced transcriptional silencing (RITS) complex. The RITS protein components have been identified in S. pombe yeast and include Ago1, the GW protein, Tas3, and chromodomain protein 1 (Chp1) [54], however while Ago1 is present in mammalian cells, Tas3 and Chp1 homologues have not yet been identified. Although this complex can be considered as the equivalent of RISC from PTGS, it is not identical, due to the different functional requirements and distinct protein components. Via siRNA sequence complementarity, RITS identifies the target locus and induces chromatin compaction through epigenetic modifications, such as histone methylation [55]. By rendering it structurally inaccessible to transcriptional machinery, TGS can lock the virus in a latent state.
\nLike PTGS, TGS is capable of significantly suppressing HIV-1 production and is highly sequence specific, with minimal off-target effects and interferon mediated immune responses or defence mechanisms, dependent on the specific sequence targeted [56]. A single nucleotide mismatch between the siRNA and its target could be sufficient to disrupt silencing. Hence, conserved regions of the provirus must be carefully selected and a multiplexed approach may be necessary. TGS also offers several advantages over its cytoplasmic counterpart. By preventing the provirus from actively transcribing, it can silence HIV-1 prior to the generation of escape variants. Additionally, due to the heritable nature of the heterochromatin marks, daughter cells exhibit the same suppressive phenotype [57].
\nOur laboratory has described two siRNA sequences capable of inducing potent TGS in HIV-infected cells. The first siRNA, termed siPromA, was identified in 2005 and has been extensively characterised as inducing highly-sequence specific TGS via epigenetic repressive mechanisms [53, 55, 56, 58, 59, 60]. The siPromA sequence targets NF-κB tandem repeat motif, which is unique to the virus and is not homologous to any host cell NF-κB motifs. This is important as NF-κB is an important transcription factor for multiple cell signalling pathways. A second siRNA, termed si143, has recently been shown to also induce TGS and targets the COUP-TF and AP-1 transcription factor sites upstream of the siPromA target sequence [61]. When combined, these two siRNAs provide enhanced suppression and enforcement of latency through multiple epigenetic modifications. We have shown up to 1000-fold suppression of virus transcription following a single transfection of siPromA or si143 for up to 15 days in various HIV-infected cell cultures, including HeLa T4+ cells and Hut78 cells. Further, in MOLT-4 cells carrying shRNA expressing siPromA, we reported virus transcription was suppressed for over 1 year [60]. Specific epigenetic modifications in HIV cultures suppressed by siPromA or si143 have been investigated using ChIP assays and included enrichment of histone methylation on the N-terminal histone tail (such as HeK27me3, H3K9me2) and decreased acetylation (such as H3K9) [61]. We have also demonstrated effective in vivo virus suppression using a humanised mouse model of acute HIV in (NOD)/SCID/Janus kinase 3 (NOJ) knockout mice infected with HIV-1JR-FL. In human PBMCs that were stably transduced with shPromA delivered by a lentivirus vector and transplanted into the NOJ mice, followed by immune reconstitution, mice were protected from HIV-1 challenge, with significantly decreased plasma viral loads and normal CD4:CD8 T cell ratios, compared to control group treated with cells transduced with an inactive siRNA sequence carrying three mismatches (shPromA-M2) [58]. We anticipate, much like combined ART, that a multiplexed approach of combining TGS-inducing siRNAs will be necessary to provide sufficient control across a wide range of HIV subtypes and strains.
\nThe Chattopadhyay laboratory has also reported a TGS-inducing siRNA sequence specifically targeting the HIV-1 subtype C NF-κB triple repeat motif, termed S4-siRNA. They demonstrated S4-siRNA induced TGS in a TZM-bl cell line and ex vivo human PBMCs transfected with S4-siRNA and infected with various subtype C viruses, as determined by measuring viral RNA levels [62]. Further, ChIP assay confirmed the enrichment of epigenetic repressive marks using histone methylation markers, H3K27me3 and H3K9me2. This siRNA may have potential as an RNA therapeutic, since HIV-1 subtype C is prevalent in approximately half of the people living with HIV globally.
\nThe Morris laboratory has also described a TGS-inducing siRNA, termed, LTR362, which also targets the NF-κB tandem repeat motif [63] and overlaps with 8 bp of the siPromA sequence. This RNA therapeutic has recently been further developed with the addition of a delivery aptamer designed to the HIV-1 glycoprotein termed gp120 A-1 and multiplexing with PTGS-inducing siRNAs targeting Tat and Rev. [64], designed by the Rossi laboratory. They showed the LTR362 RNA localised to the nucleus of an HIV-infected T lyphoblastoid CEM cell line and primary human CD4+ T cells. Virus suppression showed a 10-fold reduction of viral p24 levels compared to control cultures at 12 days post-infection. This potential dual therapeutic was assessed in vivo using an HIV-1 infected humanised NOD/SCID/IL2 rγnull mouse model and demonstrated suppressed virus infection and protected CD4+ T cell levels in viremic mice. However, the mechanism of virus suppression was determined to be PTGS, due to the lack of the CpG methylation, an epigenetic silencing mark, at the 5’LTR. Investigation of histone methylation may prove some involvement of TGS, however the study currently indicates that while cell-type specific aptamer delivery of TGS-inducing siRNA functions in vitro, the in vivo silencing effect will require significant optimising to achieve robust epigenetic modifications [64].
\nThe first person cured of HIV-1 was Timothy Ray Brown, also known as the ‘Berlin patient’, who still today remains to be the only person to be cured of HIV-1. Diagnosed with HIV at the age of 29, the patient commenced ART [65], but then presented with acute myeloid leukaemia at the age of 40. At that stage the patient’s HIV was controlled with ART and classified as stage A2; asymptomatic with a CD4+ T-cell count of 415 cells/μL [66]. ART interruption during the first initial treatment showed viral rebound, therefore ART was resumed and no further treatment was required until an acute myeloid leukaemia relapse 7 month after initial treatment [66]. The patient then received an allogeneic haematopoietic stem-cell transplantation (HSCT) [65, 66]. HSCT was already shown to be feasible in HIV positive patients, but it was also known that HSCT alone was insufficient to eliminate HIV [67]. For many patients finding an HLA-matched stem-cell donor is a significant challenge, however a suitable match was identified for the Berlin patient and subsequent screens for possible donors with the homozygous CCR5-delta32 (CCR5Δ32/Δ32) allele were performed [65, 66]. High resistance against HIV infection has been reported for individuals who are homozygous for the CCR5-delta32 deletion [68, 69]. HIV requires CD4 and typically either CCR5 (or CXCR4) for cell entry, making it a promising candidate for intervention [69]. Unlike CD4 and CXCR4, the absence of CCR5 is not obviously deleterious for modified cells [69]. Therefore the approach to use CCR5-delta32 stem-cells for HSCT of HIV infected patients was pursued, as earlier described by Chow et al., in 2001 [70]. Using this treatment approach an HLA-matched stem-cell donor with the homozygous CCR5-delta32 allele was identified [66].
\nThe patient ceased ART medication on the day prior to the HSCT procedure, which was successful with complete chimerism achieved and only grade I graft-versus-host disease (GvHD) as serious complications [66]. HIV infection was analysed by RNA and DNA-PCR and remained undetectable in peripheral blood and bone marrow, as well as in the rectal mucosa [66]. Analysis of macrophages in the intestinal mucosa found they were still expressing CCR5, indicating that 159 days post-HSCT these long-lasting cells were not yet replaced by the new immune system [66]. The CD4+ T-cell count in peripheral blood stayed at a low level of less than 300 cells/μL after the first HSCT until leukaemia relapsed on day 332 after HSCT [66, 71]. Following a total body irradiation, the patient received a second HSCT from the same CCR5Δ32/Δ32 donor [66, 71]. Fortunately, after the second transplantation the HIV load remained undetectable for the following years in peripheral blood, bone marrow and tissue biopsies, including gut and brain [66, 71]. CCR5-expressing macrophages in the gut became undetectable over the years and the peripheral CD4+ T-cell count increased greatly within the first 6 month after the second HSCT, to over 400 cells/μL [71]. While the treatment was successful in inducing remission from the acute myeloid leukaemia, recovery from the second HSCT was slow, with a long period of infections, GvHD reactions in the liver and a period of fever, dizziness and delirium [65, 71]. The patient experienced loss of short-term memory, was almost paralysed and had to learn to walk again [65, 71].
\nAs a milestone in HIV cure research, there is the question if this is a one-time wonder cure or if it is reproducible? In 2014, Hutter et al. assessed six more cases of patients with HIV-1 receiving an allogenic CCR5Δ32/Δ32 HSCT [72]. Five of those patients died within the first 4 months due to relapse, GvHD or infection [72]. The only patient surviving for 12 months experienced a rebound of CXCR4-tropic HIV-1 rapidly after the transplantation and died from a relapse of cancer [72]. This shows the difficulties of HSCT in HIV infected patients and the importance for careful selection of donor to recipient, as well as considering the continuance of ART to prevent CXCR4-tropic HIV-1 from rebounding until the new immune system has become more established [72]. In light of these attempts to replicate the successful treatment of Timothy Ray Brown, it should be noted that he was in fact Δ32 heterozygous prior to his HSCT, which likely provided him an advantage in relation to providing protection via Δ32 expression after transplantation.
\nThe mechanisms of Berlin patient HIV cure are currently being investigated and pose an interesting question-is it a functional or sterilising cure? To start to answer this question, we will likely only be able to use the information currently available, as ongoing updates on this case may be limited due to the patient recently commencing pre-exposure prophylaxis (PrEP) in order to prevent contracting HIV a second time. Firstly, the patient had ceased taking ART for >4 years without experiencing viral rebound, secondly, the viral DNA level was below detection limit in the periphery and in tissue biopsies, and thirdly, the patient showed a decrease in anti-HIV antibodies, all indicating a lack of virus replication, which makes it possible to conclude that the patient is functionally cured of HIV [66, 71]. The principle of a sterilising cure is the complete eradication of a pathogen out of the human body. This would therefore mean that every single cell previously infected and therefore carrying the HIV-1 genome would need to be replaced by new donor-derived cells to completely eradicate HIV from the body. All tests for proviral DNA until now, showed no detectable HIV-DNA and Timothy Ray Brown remained without viral rebound for 4 years, indicating the possibility that even the long-lasting memory immune cells were replaced by cells derived from the donor. This could lead to the interpretation that it was in fact a sterilising cure. That being said it is important to take into account the current limits of detection and the fact every single cell in his body cannot be analysed. Further similar results were found in two other patients who did eventually rebound. Therefore, one cannot be definitive in whether the cure is functional or completely sterilising. Regardless of whether the final conclusion is potentially a sterilising cure, it was derived from a functional cure approach.
\nThe ability to engineer specific changes in the genome of an organism has developed rapidly over the last 10 years. The technology of gene editing relies on nucleases, scissor-like enzymes, with the ability to cut genomic DNA in a highly specific manner. This process results in additions, deletions or alterations at the targeted site of the genome. There are two pathways that can achieve double stranded breaks (DSB) in DNA; (i) nonhomologous end joining (NHEJ) repair pathway, where deletions or insertions in the target gene result in gene disruption e.g. CCR5, or (ii) the homologous recombination or homology-directed repair (HDR) pathway, in which DNA sequences are introduced into the genome using a homologous DNA template. The HDR pathway is more precise, with limited off-target genome effects, due to more control over the integration site, copy number and expression of the DNA sequence [73]. Some examples of gene editing technologies include zinc finger nuclease (ZFN), transcription activator-like nucleases (TALENS), clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9). Examples of specific gene editing technologies that have been explored in the context of HIV treatment are described below.
\nZFNs are a combination of zinc-finger proteins, which have DNA recognition specificity, and the nuclease activity of the cleavage domain of restriction enzyme, FokI. The most advanced ZFN pair targets the CCR5 host gene and HIV-1 co-receptor [74]. Preclinical studies in a mouse model, where mice received CD3/CD28-activated primary CD4+ T cells treated with the ZFN delivered in a chimeric adenoviral vector, Ad5/F35, showed anti-HIV efficacy, with disruption of 40–60% of all CCR5 alleles and 33% disruption of both CCR5 alleles [74]. Following the successful pre-clinical data, the ZFN progressed to phase I clinical studies and the first-in-human gene editing HIV treatment trial (#NCT00842634) commenced in 2009.
\nThe primary outcome of this study investigated the safety of ZFN modification of autologous CD4+ T cells being delivered to HIV positive individuals [75]. A secondary outcome measured immune reconstitution and HIV resistance. Twelve ART-treated patients with undetectable viral loads were enrolled in two cohorts dependent on CD4+ T cell count; cohort 1 included patients with CD4+ T cell count >450/mm3, the median being 662/mm3, and cohort 2 was patients with lower CD4+ T cells counts between 200 and 500/mm3, the median being 272/mm3 [75]. Patients received one infusion containing 5x107 autologous CD4+ T cells that were ZFN-modified. The infusion of ZFN-treated cells was deemed safe, with one serious adverse event reported that was infusion-related. All patients demonstrated engraftment, with the ZFN-modified cells being present for up to ≥42 months following infusion and showed expected characteristics. At 4 weeks post-infusion, cohort 1 ceased taking ART in a 12 week analytical treatment interruption (ATI), resulting in four out of six patients with detectable viral loads at 2–4 weeks post-ART cessation [75]. One of the six patients experienced a delayed increase in viral load at week 6, but was still below the viral set point [75]. This patient was later determined to be heterozygous for CCR5Δ32, suggesting that this genotype enhanced the ZFN treatment effect.
\nThe successful modification of CD34+ HPSCs was also shown using the same ZFN pair [76]. This has been further optimised to achieve HDR-induced gene modifications using an adeno-associated virus vector (AAV) serotype 6 and electroporation to deliver nuclease mRNA to both primary CD4+ T cells and HPSCs [77], achieving between 8 and 60% and 15–40% CCR5 editing, respectively. Currently, there is no in vivo method that can effectively deliver nucleases to cells infected with HIV, and this will require further characterisation of the HIV sanctuary sites and identification of latent cell markers to allow specific targeting of cells that comprise the virus reservoir.
\nThe generation of off-target genome modification is also a concern for the clinical application of ZFNs. This is exemplified by the off-target effect reported for the highly related CCR2 gene which was disrupted in 5.39% of ZFN-modified CD4+ T cells that were targeting CCR5 and decreased CCR5 expression by 36% [74]. Optimisation of the CCR5 ZFN would be required if this off-target effect was determined to be deleterious.
\nThe development of therapeutics using CRISPR/Cas9 technology has rapidly intensified over the last decade. The system is based on a short guide RNA (gRNA) that targets a specific DNA sequence and the Cas9 endonuclease, which then cleaves the double stranded DNA. Mutations, either deletions or insertions, are introduced into the target sequence following DNA repair by the NHEJ repair pathway. Similar to ART, HIV has been shown to develop virus escape mutations when only a single gRNA is utilised and requires multiple gRNAs to prevent the emergences of virus resistance [78, 79]. Multiple studies have reported HIV-1 inactivation using this gene editing platform with dual gRNAs, via mutation at either target sites or complete excision of the virus sequence between the two target sites [80, 81, 82, 83]. Although CRISPR technology has proven successful in inactivating HIV-1, like the ZFN system, successful delivery to all cells of the latent reservoir will be challenging without a known latency marker.
\nThe CRISPR/Cas9 platform has recently been adapted to activate HIV transcription, akin to the “shock and kill” approach. This involves a mutation in the Cas9 catalytic domain which results in deactivated Cas9 (dCas9). The dCas9 can then be coupled to a strong transcription activation domain (AD) and targeted to the HIV-1 LTR can induce transcriptional activation via recruitment of transcription and chromatin modifying factors. One example of the dCas9-AD system is dCas9-VP64, which contains multiple copies of the herpes simplex virus (HSV) VP16-drived minimal AD and has been shown to activate HIV-1 promoter-driven gene expression. Interestingly, the most promising gRNA target in the HIV-1 5’LTR is single guide (sg)362F [84] and similar to the siPromA sequence described in the “block and lock” approach, targets the NF-κB binding motif.
\nDelivery of gene therapy to a specific target cell is another current challenge for an HIV cure. Viral vectors have become a regular method by which to deliver therapeutic genes and constructs [85]. There are multiple viable types of viral vectors that have been proven to be safe, relatively easy to construct and modify, and in the case of lentiviral vectors, these have the potential to transduce cells in a non-proliferative state [86]. Although the latter feature does not extend to non-proliferative leukocytes, due the presence of lentiviral restriction factors at and below the membrane.
\nOne significant obstacle to the effective delivery of sufficient quantities viral vector is the ability to transduce sufficient quantities of target cells. To overcome this, apheresis is performed in order to concentrate the desired cells. Currently, gene therapy protocols for HIV first require the isolation of the desired cells to be modified, typically following apheresis [85]. Apheresis is the process of removing mononuclear cells from blood and returning neutrophils, platelets, plasma and red blood cells to the donor. This process is performed in order to collect more of the desired cells of the blood than could be separated from a unit of whole blood of ~550 mL. While CD4+ T cells are the main target for HIV infection, other cells such as dendritic cells, macrophages, monocytes and to a lesser extent, haematopoietic stem cells (HSC) have been found to be susceptible to HIV infection [87, 88, 89]. It is known that if HSC are transduced, or modified in any way, then a wide range of subsequent immune cells including macrophages, dendritic cells, CD4+ T cells and NK cells will carry that modification [90]. While transduction of CD4+ T cells will result in only CD4+ T cells being modified, the approach of transducing HSC provides protection from HIV to a broader range of cell-types, making it a highly desirable target for treatment/modification. Once a large volume of cells has been collected over several hours, they can then be transduced with the desired viral vector and reintroduced to the individual where the cells will migrate back to peripheral blood, lymph nodes, and bone marrow. This delivery method has been used in dozens of clinical trials and has become a widely accepted method for delivering viral vectors to large numbers of cells, in particular to HSC in the bone marrow [85]. The ex vivo gene therapy process is depicted in Figure 4.
\nGene therapy delivery strategies; ex vivo lentivirus transduction of isolated patient haematoepoetic stem cells (HSC) and/or CD4+ T cells to deliver the gene modification versus systemic, in vivo delivery of the gene therapy directly to the patient, which requires a cell specific moiety to ensure targeted cell delivery.
As HSC predominantly reside in the bone marrow, in order to increase the quantity of HSC in peripheral blood, it is common to use granulocyte colony stimulating factor (G-CSF) as a mobilising agent to encourage recirculation of HSC. This causes cells to migrate from the bone marrow and lymph tissue into the peripheral blood. The use of G-CSF or other stimulating factors is essential when HSC are to be transduced with the therapeutic gene/vector, with various trials showing that HSC cell counts in peripheral blood increase 20–50-fold over the course of G-CSF administration [91, 92, 93]. To aid with re-engraftment of HSC back into the bone marrow after transduction, a technique known as myeloablation has been utilised in some clinical trials prior to the reintroduction of HSC via infusion, in order to provide an immunological niche and improve engraftment of the gene-containing cells [94]. This procedure involves the eradication of resident HSC, thereby reducing the population of non-transduced cells, and creating more space for the transduced cell population to reconstitute the bone marrow. A delay of the presence of newly ‘protected CD4+ T cell’ population would be expected due to the required production of cells, thus delaying the effect of the therapeutic gene(s). Production of new CD4+ T lymphocytes from the thymus has been predicted to be at a rate of approximately 1.65 cells/μL of blood/day due to thymic function [95]. The resulting in the production of a stable population of protected cells would be expected to gradually create a positive impact on CD4+ T cell number and help suppress viral load.
\nWhile the modification of HSC has the benefit of long-lasting and broad-spectrum protection via the differentiation of stem cells, this approach still lacks the immediate benefit of targeting the existing CD4+ T cells population. The use of CD4+ T cells as a target for HIV gene therapy has been explored and assessed in several studies. Isolation and modification of CD4+ T cells is relatively simple, as they largely populate and consistently traffic through peripheral blood. Accordingly, no stimulatory factors (such as G-CSF) are required to mobilise them prior to collection. This method has the benefits of providing an immediate benefit via the reintroduction of a protected population of the primary target cells for HIV infection [96]. This has been performed and shown to be both safe in treatment, and effective in delivery of the therapeutic gene [96, 97].
\nLentiviral vectors are being increasingly used in clinical trials to treat a variety of diseases ranging from cancers, to genetic diseases such as haemophilia and sickle cell anaemia, as well as several trials treating HIV. The largest such trial in HIV gene therapy demonstrating the safety of lentiviral vectors was the Phase II trial whereby a Tat/Vpr specific anti-HIV ribozyme (OZ1) or placebo was delivered in autologous CD34+ haematopoietic progenitor cells. The trial involved 74 patients where there were no adverse events related to the vectors or infusion process [98].
\nAs outlined above, present gene therapy efforts to target HIV are primarily defensive in approach, as they encode future HIV resistance and may not influence the HIV reservoir in the short-term. Given the success of CAR T cell therapy in various cancer trials, many investigators are now multiplexing HIV resistance alongside a CAR construct that can target HIV. As cellular markers for the HIV reservoir are often shared in various leukocyte niches, the equivalent to the anti-CD19 approach used in B cell leukaemia has yet to be determined. Rather, investigators have now turned to potent broadly neutralising antibodies, which have been screened and cloned from various HIV positive patients and target HIV envelope. In this setting, several pre-clinical studies are underway in non-human primates in the laboratories of Kiem and Jerome, where resistance afforded by C46 and shRNA is complexed with one of several CAR modules that incorporates the single variable change of well characterised broadly neutralising antibodies [99].
\nIn contrast, work led by the Berger laboratory has taken a similar but different approach to CAR T cell development. Rather than incorporating a neutralising antibody, they have complexed the first Ig-Like domains of CD4 with the serum mannose binding lectin [100]. This approach enables global recognition of HIV envelope, as it engages the CD4 binding site and also the abundant glycosylation sites that decorate the antigenic silent face of HIV Env. In the CAR T cell context, CD8+ T cells are generated alongside HIV resistant CD4+ T cells to mediate attack on the HIV reservoir. The only problem with the latter approach is that the major HIV reservoir in vivo resides in the germinal centers of secondary lymphoid tissue and actively excludes CD8+ T cells, given they lack the germinal homing receptor CXCR5. Therefore, whilst CD4+ T cells may transverse the germinal center, CD8+ T cells will not. To overcome this lack of secondary lymphoid targeting of CAR CD8+ T cells, the Skinner laboratory has recently developed a hybrid CAR construct that encompasses not only HIV targeting, but also the CXCR5 receptor [101]. In theory, this enables CD8+ T cells not only to target the HIV reservoir, but also transverse the site where the reservoir is located.
\nOne vector of note that has been extensively studied is the Cal-1 vector, which uses both the maC46 fusion inhibitor and shRNA-CCR5. This construct has been extensively studied, consistently showing therapeutic benefits in vitro. Additionally, this construct has also shown its enhanced efficacy when compared against individual genes, as the effect is induced by the use of two therapeutic targets [102]. This has not only led to stronger protection from HIV infection, but also is likely to result in reduced risk of mutation resistance [102]. This has been examined in mouse studies and non-human primates, where it has shown safety, high levels of engraftment (including in CD34+ cells), and a selective growth advantage [102, 103, 104, 105].
\nThe Cal-1 therapeutic construct is currently undergoing Phase I/II clinical trials [106]. The study involves 12 HIV positive patients, which have undergone transduction of both HSC and CD4+ T cells with a lentiviral vector carrying both the shRNA-CCR5 and C46 fusion inhibitor. The patients were divided into 3 equal groups, group 1 received no busulfan pre-conditioning, group 2 received 4 mg/kg busulfan, and group 3 received 2 doses to a total of 6 mg/kg busulfan conditioning. This study is currently ongoing but will provide important data on the optimised conditioning treatment to guide future treatment studies.
\nWhilst we now have therapeutic approaches that can focus our efforts on a HIV cure, delivery of these components still presents a barrier. Lentiviral vectors have proven to be extremely useful in providing delivery of therapeutic genes, although there are still limitations. As mentioned, cells can only be modified ex vivo, thus requiring apheresis. Additionally, in the case of HIV, as ART will prevent uptake of the lentiviral vector, patients must first stop ART, thus raising various health concerns and ethical obstacles. Furthermore, current approved lentiviral platforms can only transduce T cells that are activated, as this over comes lentiviral restrictions at the membrane and underneath the membrane. The sum of these problems significantly increases the cost of the clinical approach. In the setting of CAR-T cells the estimates for treatment of refractory B cell leukaemia is approximately $US400,000. Given this substantial cost, the accessibility of this type of therapeutic intervention is low. Thus, efforts are underway that will improve the process of this gene delivery pipeline. For instance, lentiviral vectors could be developed to target fresh leukocyte populations ex vivo, obviating the need for large scale apheresis. Additionally, lentiviral vectors could be modified to target leukocyte subsets, so the cells with the greatest stem-like attributes are re-infused and not diluted with cells that may not proceed down the differentiation pathway. This could potentially include a sub-population of resting T cells (e.g. Stem T cells) being isolated, genetically modified and re-infused in a manner that may not require apheresis. However, whether re-infusion of a smaller population of stem T cell would result in the same outcome that maybe achieved with a large population of bulk T cells obtained by leukopheresis needs to be thoroughly investigated.
\nThe ex vivo delivery of an HIV gene therapy treatment will only be achievable in developed countries with the appropriate resources to facilitate the approach and this will not be feasible in countries which currently have the largest burden of HIV, such as sub-Saharan Africa. We and others are working on an alternate and highly relevant systemic, in vivo approach, which may ultimately be accessible to all. This approach utilises nanotechnology to deliver the HIV therapeutic to target cells, ideally those of the latent reservoir. According to the Recommendation of the European Commission in 2011 the currently accepted definition of a nanoparticle (NP) is a particle where one or more external dimensions is in the size range of 1 to 100 nm [107]. However, larger particles with sizes up 1000 and 2000 nanometres are commonly referred to as ‘nano’, especially since for medical purposes the size range of ≤100 nm is not always practical, as a larger surface can carry more drug on a single particle [108, 109]. However, to be able to be used in the human body, NPs must be biocompatible and without cytotoxic side effects [108, 109].
\nConcentrating on HIV drug delivery, NPs have the unique feature of being able to absorb and carry other compounds on their relatively large functional surface [109]. Using NPs as delivery agents has the potential advantages of highly specific and controlled drug delivery to a targeted tissue or cell, such as those of the latent reservoir, keeping non-target organs and cells free of the drug, thereby reducing toxicity. Further, by releasing the drug in a controlled manner at a predetermined rate, achieved through changes in the physiological environment like pH, temperature or enzymatic activity, the resulting therapeutic efficacy can be increased [108, 109, 110]. Importantly, nano-based delivery systems have been shown to transport therapeutics across the blood-brain barrier, which is highly relevant for treating neuro-degenerative diseases and specifically the HIV reservoir in the central nervous system [111]. Prior to use of NPs in humans, the following basic prerequisites need to be known: drug incorporation and release, formulation stability and shelf life, biocompatibility, biodistribution and targeting, potential toxicities as well as functionality [109]. Another consideration are the possible adverse effects of residual material after drug delivery, therefore biodegradable NPs with a limited life span are optimal [109].
\nThere are many types of NPs reported as delivery vehicles for HIV therapeutics, such as liposomes, micelles, polymer capsules, inorganic gold particles and dendrimers [112]. The number of different formulations of NPs being explored for HIV and other diseases is steadily increasing and a focused review on nanoparticle systems is provided by Pelaz et al. [112]. An example of the in vivo gene therapy process is depicted in Figure 4.
\nIn the case of HIV, NPs have been used to deliver antiretroviral drugs or anti-HIV therapeutics, such as siRNAs. Inorganic gold particles delivering antiretrovirals have progressed through to in vivo delivery in mouse models, as have poly(amidoamine) PAMAM dendrimers and RNA-aptamer conjugates (as previously describes in Section 3.2.2.2), that deliver a combination of anti-HIV siRNAs. The gold particles and PAMAM dendrimer nano-platforms will be discussed below to highlight the challenges of targeting the HIV latent reservoir.
\nThe NP platforms delivering an antiretroviral drug were comprised of inorganic gold nanoparticles particles (AuNPs) ~2–10 nanometers in diameter and were conjugated with an HIV integrase inhibitor, raltegravir [113]. Modification of raltegravir was necessary to link the inhibitor to the gold nanoparticle and involved incorporation of a thiol group to generate thiolated raltegravir. Cellular uptake and toxicity of AuNps was assessed in three different cell types; PBMCs, macrophages and HBMECs and confocal microscopy showed AuNPs inside all three cell types 24 hours post-delivery [113]. No toxicity was observed between 24 and 72 hours post-delivery. Importantly, the study investigated in vivo delivery of AuNPs and reported the presence of AuNPs in multiple sites, with the highest to lowest levels observed in spleen, liver, kidney, tail, heart, blood, lungs, muscle and brain of BALB mice 24 hours post-delivery [113]. Accumulation of AuNPs in the spleen and liver was attributed to reticuloendothelial system clearance, which is the bodies first line of defence for any in vivo delivered therapy. The lack of a specific marker for the latent reservoir is an ongoing challenge for targeting cells which harbour integrated HIV DNA and have the potential to reactivate and produce productive virus. Although this approach does successfully penetrate some cells of the latent reservoir, i.e. lymphocytes and macrophages, and to a modest degree cells in the brain, it is not a targeted approach and will most likely need further development of functional groups to penetrate the majority of cells of the latent reservoir. Due to the rarity of cells harbouring latent provirus, which is estimated to be 1 in every 106 cells, targeting these cells is the current challenge for an HIV cure.
\nThe cationic PAMAM dendrimer NP system is comprised of highly branched, chemical polymers with cationic primary amine groups on a spherical surface that form stable, uniform nanoscale complexes. The PAMAM dendrimer interacts electrostatically with negatively charged dicer substrate siRNAs (dsiRNAs). The combination of anti-HIV siRNAs in this study included tat/rev, as well as the siRNAs targeting the CD4 and TNP03 genes [114]. In this study, humanised Rag2−/−γc−/− mice were generated and infected with HIV-1NL4-3 12 weeks following engraftment, then dendrimer-siRNA complexes were delivered via i.v. injection using equal amounts of all three siRNAs. Injections were continued weekly for 4 weeks. A significant decrease in HIV viral load by 3 logs relative to controls was reported and persisted up to 3 weeks post-treatment, however virus rebound was observed in the majority of animals after this time point [114], as is the standard response in patients following ART cessation. The study then investigated redosing of the dendrimer-siRNA complexes 3 months following the last administration and observed a further virus suppression which persisted for 3 weeks past the additional treatment. Assessment of mRNA levels of the three targeted genes (HIV tat/rev, CD4 and TNPO3) showed reductions in mRNA levels relative to the controls corresponded to the dosing schedule and confirmed sequence-specific and efficient gene silencing [114]. The main challenge with this approach is the need for continual treatment, or alternately the further development of a sustained-release approach. Further, whether this approach will be able to target very rare cells harbouring the latent reservoir remains to be investigated.
\nThe field of HIV gene therapy is rapidly evolving, with development of both novel anti-HIV therapeutics and delivery systems to ensure cell specific targeting. While an ex vivo gene therapy approach for HIV is well on the path to patient translation, further targeting of the latent reservoir will be necessary to achieve a systemic, in vivo gene therapy approach. This will require identification of biomarker/s for latently-infected cells and novel ways to incorporate them into viral vectors and/or nanoparticle platforms. Once achieved, the next challenge will be the cost of treatment, which is becoming a driving factor in the context of HIV, as the global burden of HIV is predominantly in sub-Saharan Africa. The cost of ex vivo gene therapy approaches is prohibitive in developing countries and in vivo nanoparticle approaches, whilst more cost effective, do not yet achieve sustained virus remission. Further optimisation and refinement of current delivery systems is required to enable wide scale application of a functional HIV cure.
\nThis work was supported by a National Health and Medical Research Council Project Grant APP1128012.
\nThe authors declare no conflict of interest. GS, AK and CA hold patents for si/shRNA protection against HIV-1 and GS is an employee of Calimmune Inc./CSL.
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