Main biochemical characteristics among some species of the genus
\r\n\tThe WHO classification in 2007; was based on the histogenesis and cell origin of the tumor. In the latest classification made in 2016; to better characterize the tumor and obtain better data on its prognosis; The combination of molecular and genetic biomarkers and histopathological features of the tumor was used. Despite all current treatment approaches, the median survival time is around 12 months in most GBM patients. Compared with the situation of some types of successfully treated cancers; the survival time of GBM patients is not at an acceptable level today. In the treatment of CNS tumors; surgery, chemotherapy, and radiation treatments (x-rays, gamma rays, electron and proton beams) are used. The therapeutic potential of chemotherapy; New strategies are needed to increase drug concentration at the diseased site, as this largely depends on the ability of the chemotherapeutic agent to achieve effective concentrations at tumor localization. Based on our better understanding of the genetic and molecular characteristics of CNS tumors; Targeted therapies, including vaccines, and treatment protocols such as immunotherapy are promising developments.
\r\n\r\n\tThis book supposes to be written by many authors who have an internationally honored place in their field to share their ideas about the treatment of CNS tumors. Surgery, Radiotherapy, Chemotherapy and Antiangiogenic Therapy Protocols, Immunotherapy, Molecular Therapy, Specific target-agents therapy with Nanoparticles and Gene Therapy for CNS tumors among the book chapters.
\r\n\tIn these sections; there are many practical pieces of information that can help the students who graduated from the Medicine Faculty and specialist doctors who are interested in Neurosurgery.
Due to their combined superior chemical and physical properties, carbon nanotubes (CNTs) are recognized to have a huge potential in many fields of applications (Ajayan, 1999; Rao et al., 2001; Dai, 2002; Van Noorden, 2011). These molecular-scale tubes of graphitic carbon are one of the stiffest and strongest fibers known. Besides, they have remarkable electronic, optical, thermal and chemical properties. For these reasons their interest in both academic and industrial areas is unique. Nevertheless, the as-produced material is extremely difficult to process. Development of CNT-based devices or composites of interest for new applications has been consequently hindered. CNTs are hydrophobic and incompatible with a majority of solvents, including monomers and polymers; they indeed have a high tendency to agglomerate. Moreover, CNTs and especially single-walled carbon nanotubes (SWNTs) are assembled in bundles of generally several tens of tubes. Development of efficient processes and chemical treatments that are able to control the quality of the CNT samples and to induce both their dispersion and partial or complete debundling remains highly challenging.
CNTs can be produced using different methods that basically consist in heating carbon-containing solid or gas. On the contrary to the preparation of multi-walled carbon nanotubes (MWNTs), SWNT growing requires a metal catalyst. The characteristics of the samples depend on the control and the choice of the experimental parameters used for the synthesis. A better understanding of the growth mechanisms has permitted the development of mass production processes (Grobert, 2007). Nevertheless, their uniformity (length, diameter, chirality), the quality of their walls (number of defects) and also their purity are still partially controlled. The quality of the samples has to be improved in order to benefit of the exceptional properties of CNTs in new materials. Depending on the type and the synthesis method, the CNTs can differently behave through the applied chemical treatments. Whatever the synthesis method, CNT samples persistently contain several kinds of heterogeneities: (i) carbonaceous species like fullerenes, amorphous carbon, graphitic and carbon particles, …; (ii) impurities such as residual metallic catalyst often protected by more or less graphitized carbon shells or polyhedra; (iii) defects at the CNT surface or oxygenated grafted functions, (iv) dispersion in diameter, chirality and morphology (aspect ratio) and (v) aggregation into bundles. These heterogeneities represent a major obstacle for both the establishment of universal behaviors and the development of efficient processing methods.
Nano-scaled particles exhibit an enormous surface area being of several orders of magnitude larger than that of conventional fibers. This surface area can potentially act as a powerful interface but it is also responsible for the high tendency of CNTs to form agglomerates. It appears that the commonly used procedures for manufacturing composites with conventional fibers or other-carbon-form do not show the hoped results. Indeed, CNT samples particularly and unusually behave. Hence original chemical treatments and processes have to be proposed and optimized (Kuzmany et al., 2004; Tasis at al., 2006; Karousis et al., 2010); and efforts have to be made to disperse the CNTs prior to their incorporation within the chosen surrounding medium to obtain the desired device or material.
In this paper, we first give an overview of the characterization techniques commonly used to follow surface and structural modification of CNTs upon chemical treatments; the respective sensitivity and the limits of each technique are also briefly discussed. The second part is dedicated to the description of the main kinds of CNT samples (obtained from different synthesis methods) and the question of their purification is in particular considered. In the following section, after giving the parameters that are relevant regarding chemical treatments to process CNTs, we will focus on the treatments commonly used to induce the dispersion of the CNTs in a surrounding medium (solvent, monomer or polymer) and the methods leading to modify the CNT reactivity. The last part reports on the elaboration and the characterization of CNT-based composites taking into account their particular multi-scale character.
Characterization of CNT samples is a difficult task. Their inherent heterogeneity (discussed in the following part) is one of the main reasons for that statement. It is complex to obtain an unambiguous knowledge of their behavior based on the recorded data from one characterization technique. The usually and unavoidable employed approach is the use of several complementarily techniques. The most common analytical techniques used to characterize chemically modified CNTs are transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetry analysis (TGA), Raman spectroscopy and XPS (X-ray photoelectron spectroscopy). Added to them, several other techniques can be used to specifically determine the nature of the attached chemical groups or their localization on CNT samples; for that purpose, TGA-MS (mass spectrometry) coupling technique, EXAFS (extended X-ray absorption fine structure) or adsorption volumetry are of interest.
The electron microscopy techniques (TEM and SEM) allow a qualitative and local examination of the morphology and the composition of the samples; by increasing the number of the observed zones, they can be reliable in the determination of the behavior of the CNT samples (Monthioux et al., 2001). The accuracy of electron microscopy being in constant progress, it permits to go further in the structural details of the analyzed species, including CNTs. EDS or EDX (energy-dispersive X-ray spectroscopy) for the elementary analysis at a specific location of the sample during observations can be used to determine the metal content after a purification process or the presence of one specific element belonging to the grafted functions. The main advantage of these techniques is that they allocate to separately analyze the behavior of the CNTs, the carbonaceous or catalytic impurities.
The TGA examines the weight lost of the CNT samples as a function of the temperature (usually from room temperature to 800°C-1000°C). In oxidative conditions (air or oxygen), the recorded weight loss corresponds to the combustion of the carbonaceous species of the samples. This oxidation treatment gives rise to their successive combustion as a function of their respective stability (Landi et al., 2005). At the end of the gasification process, only the oxidized catalysts remain. First, this technique is used to quantify the metal content in CNT samples after a purification process. In the example reported on Figure 1a (from Landi et al., 2005), the metallic oxides content is reduced from 9.6 wt% to 7.1 wt% after refluxing in a NHO3/HCl acidic solution for 14h. The raw sample is named ‘SWNT-HO’ and the sample obtained after reflux is named ‘SWNT-Reflux’. Second, the situation is a little bit complicated regarding the analysis of the nature and/or the content of the different carbonaceous species in the samples. Even if it is obvious that well-graphitized species will be more stable than amorphous carbon, the removal temperature range of CNTs and more- or less-ordered carbon species can be rather large and difficult to identify. However, TGA can be useful to evidence modification of CNT structure after treatments. For example, an increase of the CNT quality or concentration through a purification process or an introduction of defects through a chemical functionalization gives rise to modifications for both the recorded weight losses and the related temperature domains. It is commonly reported that CNTs decompose at higher temperature than amorphous carbon species. As illustration, Figure 1b (from Landi et al., 2005) shows the first derivative weight-loss curves for the samples previously mentioned. Raw SWNT-HO sample shows two major contributions of the weight loss with a prominent peak maximum at 427°C and a minor shoulder at 560°C, attributed to gasification of amorphous carbon and SWNTs, respectively. For ‘SWNT-Reflux’ sample, the two contributions are shifted to higher temperatures, namely, peak maxima at 503 and 598 °C, respectively.
TGA data for a raw SWNT (SWNT-HO) and SWNT-HO that has been treated using an acidic solution (SWNT-Reflux). SWNT-Reflux is obtained after refluxing raw SWNT-HO sample in a NHO3/HCl acidic solution for 14h. The TGA was ramped at 10°C/min under air at a gas flow rate of 60 sccm. a) TGA data for raw SWNT-HO (O) and SWNT-Reflux () samples. b) TGA data from the first derivative analysis of the weight loss (%/°C) for raw SWNT-HO (O) and SWNT-Reflux (b) samples. The peak maxima for the prominent thermal decomposition features are labeled for clarity. From (
As it is reported in the work of Landi and coworkers (Landi et al., 2005), the ability to assign temperature regions of combustion for SWNTs and carbon impurities would be of great importance for the optimization of purification processes. Nevertheless, a selective decomposition of carbon impurities if it exists remains an open question.
Under inert gas, TGA-MS coupling technique consists in analyzing the detachment of functions that have been initially grafted to the CNT surface (Chattopadhyay et al., 2005). The nature of the bonds created between the introduced functions and the CNTs can be identity from the release temperature domain (Lejosne et al., 2011). A molecule simply physisorbed or -stacked will be detected at lower temperature than a group which is covalently linked to the sample surface. As a complementary analysis, MS investigation allows having a feedback of the nature of the functions that were effectively attached at the sample surface.
Raman spectroscopy is a widely used technique for the characterization of CNT samples (Burghard, 2005; Graupner, 2007). It is a powerful technique because the signal of CNTs is enhanced compared to that of the carbon impurities. Several features are modified upon chemical treatments and Raman spectroscopy allows probing the quality of CNT structure, the possible selectivity of reaction with respect to the electronic properties (metallic or semiconducting) (Dyke et al., 2005) or an induced electron transfer. A typical Raman spectrum of SWNTs shows three characteristic bands: the radial breathing mode RBM (100–400 cm-1), the D mode (1350 cm-1) and the tangential (C=C vibrations) stretching G mode (1500–1600 cm-1).
At low frequency, RBM corresponds to the radial deformation of the carbon-carbon bonds. For SWNTs in bundle, the SWNT diameter can be calculated as follows (Jorio et al., 2003):
where the
The intensity of the D-band is known to be related to introduction of defects in the CNT structure. The increase of the ratio of the intensity of the D band over the intensity of the G band, ID/IG, is commonly used to prove the covalent nature of the functionalization of CNTs since the attachment of the grafted groups leads to the breaking of C=C bonds (Dillon et al., 2005). The area of the D band is also reported to be sensitive to a presence of deposit of carbon layers on CNT surface. The removal of such deposit by oxidation for example possibly leads to a decrease of ID/IG ratios (Osswald et al., 2005). Heating functionalized CNTs under vacuum or inert gas leads to the removal of functional groups and restores the initial low-defected structure. The obtained CNT samples after annealing show thus a reduced ID/IG. Figure 2 (from (Dyke & Tour, 2003)) shows three Raman spectra of raw SWNTs (A), covalently functionalized SWNTs (B) and of functionalized SWNTs after heating at 750°C under argon (C). ID/IG increases after the functionalization process because of the attachment of functional groups on the SWNT sidewalls and it decreases after heating due to the detachment of the groups and the recovering of SWNT structure.
The G band corresponds to the tangential mode of vibration of the C=C bonds in CNTs (Jorio et al., 2002). The G band is mainly composed of two or three identifiable components even if it can be usually more complex to be fitted. A simple analysis can be carried out considering the two most intense peaks that basically originate from the symmetry breaking of the tangential vibration when the graphene sheet is rolled to make a cylindrically shaped tube. They are labeled G+ for atomic displacements along the tube axis, and G− for modes with atomic displacement along the circumferential direction. The difference between semiconducting and metallic SWNTs is evident in the lineshape of the G− feature, which is broadened for metallic SWNTs in comparison with the Lorentzian lineshape for semiconducting tubes. G band can be sensitive to chemical treatments. First, for defective SWNTs after functionalization, an additional contribution, referred as the G* band, at high wavenumber is added to the conventional G band (Cataldo, 2000; Vigolo et al., 2009a). Second, reaction of SWNTs with electron-donors or -acceptors induces a shift of the G band. The reaction between alkali metals (donors) and CNTs is accompanied by an electronic transfer giving rise to an electron enrichment of the CNT structure. The G band of the obtained reduced CNTs can be then down-shifted of several tens of cm-1 (Sauvajol et al, 2003).
Raman spectra (780.6 nm excitation) of (A) raw SWNTs, (B) functionalized SWNTs, and (C) functionalized SWNTs after TGA (10 °C/min to 750°C) in argon. From (
Infrared (IR) spectroscopy is recognized to be useful for the study of SWNT sidewall chemistry. As a complementary technique of Raman spectroscopy, IR spectroscopy can be used to identify the functional groups and especially oxygenated functional groups added to the tube walls thanks to their characteristic vibrational modes (U.J. Kim et al., 2005a). The range expected for C-O stretching modes in ether, ester, alcohol, or phenol functions is around 1100 cm-1 and in the 1700 cm-1 domain, the bands can be assigned to carbonyl (C=O) stretching in ketone, aldehyde, or carboxylic acid groups. For aldehyde groups, the C-H stretching and bending vibration generally appears in the 2700-2900 cm-1 range. The O-H stretching modes are found in the 3100-3600 cm-1 range.
X-ray (XRD) and neutron diffraction techniques may help to ascertain the quality and crystalline nature of the treated CNT samples as opposed to amorphous carbon material. XRD, in particular, allows analyzing the state of oxidation of the catalyst through a purification process, for example (Vigolo et al., 2010a).
XPS (X-ray Photoelectron Spectroscopy) technique is widely used to determine atomic compositions and to qualitatively analyze different elements on the CNT surface especially upon oxidative treatments; it is also sensitive to the presence of structural defects on the nanotube surface. The C1s peak of non-treated CNTs shows a peak at 284.1 eV. The peak at 285.5 eV is attributed to defects on the nanotube structure (Datsyuk et al., 2008). Detection of oxygen-containing functional groups is evidenced by several contributions at 286.7, 288.3 and 290 eV. Finally, the –* transition loss peak is generally detected at 291.5 eV. The relative augmentation of the contributions related to the presence of oxygen with respect to that of pure carbon evidences the formation of C=O or C-O covalent bonds at the CNT surface. Figure 3 (from Liu et al., 2007a) shows that the C–O component after functionalization (SHR15) is increased compared to other components from the decomposition of C1s peak in agreement with the attached functional groups.
Decomposition of C1s curves of as-produced SWNTs (u-SWCNT), product of control experiment (pm-SWCNT) and SWNTs that have been functionalized by methoxyphenyl (-PhOCH3) functional groups through using a microwave-assisted reaction for 15 min designated as SHR15. From (
Near edge X-ray absorption fine structure (NEXAFS) can be used at the oxygen or carbon K edge to probe the bonding modification of the oxygen or carbon atoms in the sample upon a chemical treatment (Banerjee et al., 2004; Wang et al., 2010). CNT structure and chemical composition of oxygenated functional groups grafted to the CNT surface can be then analyzed using NEXAFS spectroscopy. In the case of C K-edge spectra, the CNTs are characterized by a sharp C–C * transition at 285.4 eV, three * transitions from 289.9–298 eV, and broad (+) transitions from 301–309 eV. The region between 287 and 290 eV mainly corresponds to the C–O bonding, with 287.6 eV assigned to C=O * transition and 288.2 eV assigned to C–O * transition. The O K-edge spectra are usually also analyzed as complementary data.
As it can be commonly done for porous systems, the determination of adsorption-/desorption-curves with nitrogen at 77 K can be used to characterize the specific surface area of the CNTs according to the well-known Brunauer, Emmett, and Teller (BET-method). Since a chemical treatment can induce modifications of CNT surface, such analysis can provide a valuable feedback regarding the occurrence of the chemical treatments (C.M. Yang et al., 2002).
By using gas such as xenon or krypton, the adsorption isotherms exhibit specific profile with several steps and plateaus (Arab et al., 2007; Goudon & Lasjaunias, 2008). The steps are especially related to the existence of different adsorption sites related to CNT bundle morphology; each step being positioned at a characteristic pressure different from the characteristic pressure of the carbonaceous impurities of the samples. The isotherm of a CNT sample can be considered as a signature of the surface of the CNTs themselves. It is hence possible to selectively prove the occurrence of the chemical treatment on the CNT surface (Vigolo et al., 2009b).
The CNT synthesis methods have been recently highly improved leading to the development of mass production processes (Sadeghian et al., 2009; Lehman et al., 2011). Although it is easier to produce significant quantities of MWNTs than SWNTs, their structure is less well understood than that of SWNTs because of their greater complexity and variety. Multitudes of exotic shapes and arrangements have also been observed under different processing conditions. The variety of forms may be interesting but also has a negative side because they diverge from the ideal sp2 cylindrical structure and the CNT properties are consequently diminished.
CNTs can be synthesized using both high-temperature and low-temperature processes. CVD (Chemical Vapor Deposition) process is classified in the low-temperature methods. It is based on the reaction between a carbon containing flowing gas molecules and catalyst particles often above 1000°C. Arc discharge and laser ablation methods are based on sublimation of a graphite target which occurs at relatively high temperature (1000-3000°C).
In the case of CVD methods, the growth process involves heating a catalyst material to sufficient temperatures (550-1200°C) in a tubular furnace and flowing a hydrocarbon gas through the reactor for a period of time under vacuum. The growth mechanism is based on the dissociation of carbon containing molecules which is catalyzed by a transition metal (typically Ni, Fe or Co) (Gavillet et al., 2002; Deck & Vecchio, 2005; Esconjauregui et al., 2009). After dissolution in the metal particle, a precipitation phenomenon leads to the formation of tubular carbon solids in sp2 structure. Materials grown over the catalyst, MWNTs or SWNTs, can be obtained as non-ordered soot, densely aligned bundles or as individual array deposited on the substrate (Maruyama et al., 2002; Bronikowski 2006; Singh et al. 2003; Vigolo et al., 2008
For the synthesis of SWNTs in mass quantities, particular conditions are required in the HiPco process (Nikolaev et al., 1999). It allows producing high-quality and narrow-diameter SWNTs. The metal catalyst is formed
TEM images at different magnifications showing arc-discharge as-produced SWNTs (A and B) (prepared in a home-made reactor) and SWNT synthesized by the HiPco process (C and D) (purchased from NanoIntergris Inc).
In principle, arc discharge and laser ablation are similar methods, both use a metal-impregnated or pure graphite target or electrode. The selection of which kind of CNT to be produced depends on the purity of graphite and the presence of catalyst. SWNTs could only be formed by adding metal catalysts (Fe, Ni, Y, Co) to graphite. MWNTs and also fullerenes can be synthesized when pure graphite is used instead. In a typical arc discharge synthesis, a low-voltage (~12 to 25 V) and high-current (50 to 120 A) power supply is used (Journet et al., 1997; Shi et al., 1999). An arc is produced across a 1-mm gap between two graphite electrodes of 5 to 20 mm in diameter. An inert gas such as He or Ar is used for the reaction, at a pressure of 100 to 1000 torr. The diameter distribution of SWNTs made by this method is roughly between 1.3 and 1.5 nm.
The characteristics of the CNTs (quality, defect amount, diameter distribution) and the nature of the impurities mainly depend on the synthesis method (Figure 4). Mass produced-SWNTs prepared by CVD (including HiPco) are usually more-defected and have a broader diameter distribution leading to less-ordered bundles than those obtained by high temperature synthesis processes (Figure 5 from U.J. Kim et al., 2005b). The carbonaceous nonnanotube impurities in the samples obtained by these latter are usually in larger concentration and show a larger variety of species compared to those included in the samples obtained by CVD. Both high temperature and CVD as-produced-CNT and especially SWNT samples contain metal residue coming from catalyst required for their growth. These catalysts are often protected by carbon shells and more or less graphitized carbon particles; and they are difficult to efficiently remove. This is especially the case for arc-discharge SWNT samples.
TEM images of as-grown SWNT bundle cross-section produced by an arc-discharge method (a) and (b, c) SWNT bundle from HiPco materials. Insets are schematic representations of the spatial arrangement of SWNTs in the bundle for each material. From (U.J.
Even if the growing mechanisms are now better understood, it remains difficult to achieve both high-purity and high-yield CNT samples from the production methods. The as-produced soot persistently contains a part of nonnanotube species: (i) carbonaceous impurities showing large range of size and cristallinity from completely amorphous carbon to more or less ordered or well-graphitized particles and (ii) particles of residual metal catalyst required for the synthesis of SWNT-type. Numerous treatments and procedures for mass purification have been proposed, they are based on physical processes and/or chemical treatments (Hou et al., 2008; Cho et al., 2009). The physical methods are based on the difference in size, density, aspect ratio, magnetic properties between the impurities and the CNTs. They generally involve several steps of centrifugation (A. Yu et al., 2006) or filtration which prevent CNTs from severe damage. Most of the chemical procedures involve dry or wet oxidation process and/or an acid treatment. Such treatment is difficult to render selective towards the impurities because CNT are as well sensitive to the used oxidative process (Landi et al., 2005; Sen et al., 2003; Smith et al., 2003; Martinez et al., 2003;Vigolo et al., 2010a). Rigorous optimization of the experimental parameters has to be performed and adapted to the sample source. The carbonaceous impurities showing a large range of structural organization consequently lead to a large scale of stability. Moreover, inherent heterogeneities can be responsible for non controlled behaviors. The final quality of the CNTs (concentration and sidewall-defect amount) can be high but the yield consequent to the attack and the consumption of the CNTs is often disappointing. Subsequent high temperature annealing of the samples is required to restore the cristallinity of the CNTs and to remove the functions that have been grafted at their surface by the previous chemical treatments. Figure 6 (from Martinez et al., 2003) shows TEM images of SWNT samples at a raw state (a) and after each chemical treatment of the followed purification procedure. After nitric acid refluxing (b), SWNTs appear damaged due to the introduction of defects in their structure and a possible intercalation of HNO3 molecules within the bundles. Oxidation treatments being also aggressive to the SWNTs, bundles (c) appear as well attacked. Annealing under Ar atmosphere at 950°C for 10 h (d) is able to remove the defects and the sidewall functions introduced through previous treatments and to restore the SWNT structure.
TEM images of raw SWNT material (a), nitric acid-treated sample (b), air-oxidized sample (c), and annealed sample (d). From (
A long-standing issue involving the complete elimination of the metal catalysts from SWNT soot remains to be addressed. It is recognized that these metallic impurities (especially Ni, Fe, Co) can affect both magnetic and electric properties of the CNT samples; as well as defects that can be introduced in the CNT structure during the chemical treatments (Ellis & Ingham, 2006; Kolodiazhnyi & Pumera, 2008
The assessment of CNT purity is really challenging (Arepalli et al., 2004); it does not exist a dedicated characterization technique allowing determining selectively the concentration of CNTs. The currently used techniques for the characterization of the treated samples are MET, TGA and Raman spectroscopy. TEM allows a qualitative description of the SWNT concentration and the degree of their wall damaging. TGA carried out under oxidative atmosphere is supposed to lead to an assignment and a quantification of the present carbonaceous species from the observed temperatures of removal. Nevertheless, it is difficult to discriminate from the temperature of elimination of CNTs to that of other carbonaceous species. Raman spectroscopy can help in characterizing the damaging of the tubes upon the used treatment.
CNTs are often entangled according to the production process and they have high tendency to rapidly re-aggregate if no special surface agent or treatment is used to maintain them in a dispersed state. Several means can be used to modify the CNT surface properties, indispensable step for their characterization, their manipulation, their processing or their incorporation in materials (Figure 7 from Hirsch, 2002). It can be achieved by the use of surfactants (Vigolo et al., 2000; Dror et al., 2005), polyelectrolytes (Grunlan et al., 2006), biological molecules (Qiao & Ke, 2006)… that are able to reduce the interfacial energy between the CNT sidewalls and a solvent (Niyogi et al., 2002; Hirsch, 2002; Karousis et al.,
Possible functionalization approaches for SWNTs. Functionalization possibilities for SWNTs: A) defect-group functionalization, B) covalent sidewall functionalization, C) noncovalent functionalization with surfactants, D) noncovalent functionalization with polymers, and E) filling tube cavity of SWNT, for example, C60. From (
2010). These physically adsorbed coatings are indeed able to counter-balance the van der Waals attractive forces between the CNT bundles and can also lead to the debundling and CNT individualization (Grossiord et al., 2005). Two others approaches are usually proposed to increase the affinity of the CNTs towards a surrounding media. Covalent functionalization which consists in the attachment of a chemical group, generally having a hydrophilic character, is recognized to be an efficient way to obtain well-quality CNT dispersions. An alternative soft-chemistry route which is based on an electron transfer between an alkali metal and the CNTs allows obtaining high-stable CNT dispersion. This process avoids any introduction of defects in CNT walls since only their electronic structure is modified. These two last mentioned processes are described in more details in the following sections.
The use of surfactant molecules or polymers which are physically adsorbed onto the CNT surface has the advantage of not altering the CNT surface and they can facilitate their manipulation. However, for manufacturing CNT-based composites, their removal during the process is difficult and their presence in the final materials can be responsible for diminishing the composite properties. Indeed, since the remaining molecules are situated at the CNT surface, they can drastically reduce the interaction between the CNTs and the polymer matrix. The chemical functionalization which consists in covalently grafting functional groups on the CNT surface is commonly used to induce both the dispersion of CNTs in solutions of monomers or polymers and good CNT-polymer interaction.
Covalent functionalization can be realized by either modification of surface-bound carboxylic groups situated on the CNTs or direct addition of reagents to the CNT sidewalls by radical attack for example (Sun et al., 2002; Dyke & Tour 2004b). In the first category, CNTs are simply submitted to an oxidation process using HNO3 for example (H. Yu et al., 2008). Oxygen-containing groups including carboxylic acid functions are either directly formed on intrinsic defects or are added at the CNT surface (Zhang et al., 2003). The treated CNTs can be easily dispersed in many solvents (Rosca et al., 2005; Tchoul et al., 2007) and the attached acid functions can be used as sites to attach a variety of functional groups (Niyogi et al., 2002; Wepasnick et al., 2011). In the second category, the functional groups are directly added on the CNT sidewalls without using a preceding acid attack. The developed procedures are often based on the generation of radicals that open the C=C bonds of the CNT structure. In that case, the pre-existing defects are not the favored sites but the addition mechanism rather involves an introduction of additional defects. (Dyke & Tour, 2004a; Liang et al., 2004; Mickelson et al., 1998; Ying et al., 2003). Various functional groups such as alkyl, aryl or fluorine can be covalently attached at the CNT sidewalls. Dispersability of CNTs in various solvents and in polymers can be successfully increased by using a functional group having a good affinity towards the surrounding medium. Changes in the affinity of the functionalized SWNTs towards the solvent can be merely evidenced by dispersion tests. Figure 8 shows photographs of dispersions (in DMF) of SWNT samples that have been submitted to a functionalization process in three steps. Functionalized samples are well dispersed after steps 1 and 3 (dark solution); on the contrary, the dispersion quality of SWNT-PhOH is much reduced after step 2 of the chemical process.
Photographs of dispersions of functionalized SWNTs in DMF after each step of a chemical procedure. The photographs have been taken two weeks after the dispersion preparation. From (B.
Common SWNT sidewall functionalization methodologies. From (
Numerous chemical routes have been developed; they are able to attach various functional groups at the CNT sidewalls. They are based on the use of a highly reactive intermediate which is required to attack the carbon nanotubes. As example, table 1 gives the mainly used methods. The aim here is not to enter into details for each method of functionalization but focus the discussion on the related functionalization levels: pertinent parameter for CNT-based materials. Based on its high reactivity with graphite, fluorination was chosen for initial studies (Mickelson et al. 1998). In that case, the very high functionalization level can be found since one C-F function is present every 2 carbon atoms on the CNT. The second methodology involves the well-known substitution by benzenediazonium salts; it leads obtaining CNT being less functionalized with 1 function every 10-20 carbons (Dyke & Tour, 2004a). Arylation and alkylation of CNTs often used as preliminary step for numerous functionalization procedures, can be also obtained by radical reactions for which the degree of functionalization depends on the used process for the generation of the radicals as we will see (Ying et al. 2003; Liu et al., 2007a). SWNTs can be as well modified using cyclization reactions using reactive carbene and nitrene reagents to attack the SWNT walls (Holzinger et al., 2003). Cyclopropanation of SWNTs under Bingel reaction conditions has also been reported (K.S. Coleman et al., 2003). In the case of the functionalization process developed by Billups and coworkers, the reaction leads to ultrahighly lithiated SWNTs (1 lithium atom per 2.2 carbon atoms) that can be further treated with numerous electrophiles including alkyl halides, aryl halides, and even vinyl monomer. Interestingly, functionalized SWNTs are obtained in an individualized state (Liang et al., 2004).
As we have just seen, depending on the chemical mechanism and procedure, the obtained levels of functionalization can be relatively elevated. Integration of covalently functionalized CNTs in polymer matrix could induce good stress–strain transfer between nanotubes and polymer guarantying interesting mechanical properties in composite materials (c.f. section 5). Nevertheless, the breaking of CNT conjugated π system may have negative impact on properties (conductivity, in particular) of the obtained composites (Garg & Sinnott, 1998; Byrne & Gun’ko, 2010; Bose et al., 2010). This is the reason why, for composite processing, the functionalization levels have to be controlled and maintained relatively low in order to avoid a strong alteration of the CNT structure. However, grafting degrees are not easy to master and they mainly depend on the involved mechanism of reaction and the means used to facilitate the reaction (Syrgiannis et al., 2010). Chemical reactions assisted by micro-wave are recognized to lead to higher functionalization degree than those obtained by thermally-assisted reactions(Liu et al., 2007b). The chemical procedure we have developed is based on the direct attack of the sp2 carbon on the CNT surface. It advantageously allows having a certain control of the yield of functionalization without the introduction of a large number of defects (Liu et al., 2006; Vigolo et al., 2009b). The obtained low yield of functionalization is efficient enough to modify the surface properties of the CNTs but preserve their structural integrity (Dossot et al., 2007). The other main difficulty regarding the integration of functionalized-CNTs in polymer matrix concerns the homogeneity of the functionalization degree on the CNT surface over the several milligrams of the used CNT sample for composite elaboration (Vigolo et al., 2009c). Because of the high tendency of CNTs to form aggregates, accessibility of reactants to CNT surface has to be improved by using pre-dispersion process (usually done by ultrasounds). Depending on the used solvent which is conducted by the functionalization treatment itself, the CNTs are often poorly dispersed.
Development of soft chemistry processes such as intercalation reactions is highly challenging for both dispersion and debundling of the SWNT bundles. Indeed, CNTs have demonstrated an amphoteric character since they can be doped or intercalated either by electron-donors or -acceptors (Duclaux, 2002). These reactions are accompanied by an electronic transfer that has been evidenced by means of several techniques such as transport measurements (Grigorian ‘et al., 1998; Fischer, 2002) or various spectroscopies and especially Raman spectroscopy (Bendiab et al., 2001; G. Chen et al., 2005). This electronic transfer could as well play a major role in the dispersion process of SWNT bundles. To our knowledge, only donor-type reactions with SWNTs have been successfully used for this purpose. Donor-type reactions are carried out with the strongest reducing metals: the alkali metals. Three routes are possible. The chemical reduction can be thermally assisted (i) in vapor phase or conducted (ii) in liquid phase at room temperature; or, (iii) based on an electrochemically process. The electrochemical intercalation that was mainly studied with lithium is known to induce damages of the SWNTs by progressive solvent co-intercalation. In-situ X-ray diffraction evidences an irreversible loss of the “triangular” lattice of SWNTs (Fischer, 2002). Routes (i) and (ii) do not show any alteration of SWNT structure. Light alkali metals, lithium and sodium were firstly chemically intercalated into SWNTs using the liquid phase method in THF solutions with several radical anions (naphtalene, benzophenone, anthraquinone, benzoquinone) at ambient temperature (Petit et al., 1999). Heavy alkali metals such as potassium could also be successfully intercalated by means of the same method. In that case, within the obtained compounds, intercalated alkali cations are surrounded by solvent molecules (THF, for example). Figure 9 gives a scheme of two ternary intercalation compounds and reveals that the intertube distance depends on the size of the alkali metal.
Left part: Structure of the KC5.88-THF (top) and the LiC5.88-THF (bottom) compound after energy minimization. Right part: Experimental Neutron Diffraction patterns of the KC5.88-THF sample (upper line) and the LiC5.88-THF (lower dotted line, shown for comparison). The bold line is a calculated Neutron Diffraction pattern of the KC5.88 sample involving the structural parameters described in the text. From (Cambedouzou et al., 2005) Copyright 2005 by the American Physical Society
In the case of intercalation in liquid phase, the electronic transfer was estimated from optical absorption response of the formed radical ion. Heavy alkali metals could be intercalated into SWNTs using vapor phase method (Duclaux, 2002; Duclaux et al., 2003; Vigolo et al., 2009c). The reaction temperature depends on the used alkali metal, more precisely on its vapor pressure. Contrary to the previous method, the vapor-phase process allows preparing binary compounds. The structure of the intercalated materials strongly depends on the quality and the structural parameters of the SWNT sample. For heavy alkali metals, an expansion of the 2D lattice is often observed (Duclaux, 2002). This expansion is reported to be due to the occupation of the interstitial channels. However, the precise location of the alkali metal atoms and the type of sites preferentially occupied are difficult to determine. Moreover, from different observations and measurements, it is possible to claim the absence of intercalation stages in SWNT system contrary to that could be observed in graphite intercalation compounds. The level of chemical intercalation is then difficult to control. The excess of reagent with respect to carbon materials during the chemical reactions leads generally to “saturated” materials. However, the intercalation process itself remains poorly understood because it is not a straightforward process. Indeed, it involves some modifications of both the SWNT electronic structure upon a reduction reaction and the structural parameters subsequent to the intercalation of guest species within the host structure. Two main forces oppose each other throughout the chemical process: favorable forces resulting from the electron transfer and non favorable mechanical forces acting against an increase of the 2D lattice. As for graphite intercalation compounds, the obtained structure mainly results from the balance between these two opposite forces.
AFM height image of electric arc SWNTs deposited on mica from solution after debundling process of ternary intercalation compounds prepared by a liquid medium intercalation method. Height measurements show a height of about 1 nm on all nanotubes measured consistent with the presence of isolated SWNTs in the solution. From (
Beyond the precise understanding of the intercalation process itself, the preparation of SWNT-intercalation compounds is of great interest since the obtained reduced SWNTs are able to be dispersed and partially or completely debundled. Indeed, these intercalation compounds can be assimilated to salts capable to dissolve in aprotic polar solvents such as NMP (1-méthyl-2pyrrolidone), DMF (dimethyl formamide) or DMSO (dimethyl sulfoxide) (Pénicaud et al., 2005; Vigolo, et al. 2009c). Such dissolution-like mechanism is spontaneous avoiding the need of sonication or other high energy mechanical mix methods which can damage CNTs. The obtained dispersions are highly stable as long as they are kept in inert atmosphere. Polarity is a key parameter for minimizing the mixing energy of particles in a solvent. The electron transfer between carbon atoms on the CNT surface and the intercalated metal, increasing the surface reactivity, is very helpful for the dispersion of CNTs in polar solvents. Regarding the question of the preferentially occupied sites, the dispersion stability indicates that alkali metal atoms certainly occupy the external sites and the groove sites of the bundles. Moreover, locally the presence of alkali metal atoms in the interstitial sites (between two tubes within a bundle) will favor the debundling process since the increase of the 2D lattice has already taken place. The dispersion process is then expected to lead to the debundling of the nanotubes between which alkali metals were inserted. The degree of debundling will then depend on the level of filling of interstitial channels. Indeed, if the interstitial channels are not occupied at all, preserved bundles should be observed after dispersion. On the contrary, in the case of a complete occupation of the interstitial sites, complete debundling of SWNTs leading to their individualization is observed. This is the case of SWNTs reduced by alkali metals in liquid phase (Pénicaud et al., 2005). It is then possible to prepare solution containing individualized SWNTs (Figure 10).
In the case of binary compounds prepared by vapor phase, only partial debundling is observed, explained by a non compete filling of the interstitial sites especially favored at the ending and the external part of the bundles. This partial debundling leads to hyperbranched structures of CNTs (Figure 11).
TEM images of SWNT bundles after the debundling process of binary alkali metal-SWNT compounds prepared through a vapor-phase intercalation process.
This method involving a CNT reduction reaction is of great interest since it allows the formation of individual CNTs or hyperbranched structures of CNTs reducing that way the size of the usually obtained bundles and increasing the developed interfacial surface. Obtaining transparent and conducting composites or antistatic coatings are some of the most challenging applications of CNTs. High-quality dispersions of debundled non-defected SWNTs can be of great interest for manufacturing such new CNT-based composites.
CNTs are recognized to be the ideal filler to obtain superior composites (J.N. Coleman et al., 2006). Their high aspect ratio combined to their lightness is one of the fundamental requirements for reinforcement of polymer matrices. The challenge consists in successfully transfer both the remarkable mechanical and conductive intrinsic properties of the CNTs at the macroscopic scale of the composite materials. Dispersion is known to be certainly the most fundamental issue. Efficient load transfer cannot be achieved if CNT aggregates remain within the polymer matrix; CNTs have to be randomly dispersed in bundled or isolated state. Their alignment is also reported to enhance the mechanical properties along the CNT axis (Xie et al., 2005). High interfacial strength between CNT surface and the polymer matrix is also an indispensable requirement to induce efficient stress transfer within the composites (Cadek et al., 2004; Gorga et al., 2006).
Various procedures have been developed for the preparation of CNT-based composites (Fiedler et al., 2006; Ma et al., 2010; Spitalsky et al. 2010). Functionalization is widely used to achieve both the dispersion of CNTs and a good wetting between CNTs and polymer chains. Indeed, the procedures that consist in simply mixing non-treated CNTs in the polymer system (either solubilized in a solvent or used in a melting state) present the advantage to be compatible with large-scale production but re-agglomeration phenomenon is still difficult to avoid. Two main approaches for the incorporation of functionalized CNTs in polymer matrices have been developed:
Mechanical properties of polymer composites containing functionalized CNTs. From (
Both conductivity and mechanical properties (see table 2) of composites containing functionalized CNTs cover quite a large domain (Byrne & Gun’ko, 2010). Mechanical performance of the obtained materials showing the highest Young’s modulus of 7.2 GPa (Table 2) is to a certain extent disappointing compared to Young’s modulus of the CNTs being more than 100 times higher. It is however essential to remind, that the measured properties depend on the CNT type, the treatment used to modify CNT reactivity and the used preparation technique. It is indeed difficult to directly compare the values of Young’s modulus to estimate the effectiveness of a given CNT-polymer system. Moreover, the rate of increase of the Young’s modulus (dY/dVf) as a function of the concentration of CNTs within the matrix is reported to be a most pertinent parameter for a quantitative evaluation of the induced reinforcement (Cadek et al., 2004). The interfacial area in CNT-based-materials plays a fundamental role in the reinforcement mechanism and dY/dVf indeed quantifies the efficiency of the interfacial area to reinforce the obtained materials (table 2).
Micromechanical mechanisms for the reinforcement phenomenon in CNT-based composites are not easy to ascertain due to the multi-scale character of the materials. The interfacial stress transfer could be analyzed by means of fiber pullout model. If adhesion between CNT surface and polymer matrix is efficient, crack propagation tends to be inhibited by a bridging phenomenon increasing the toughness of the material. Reinforcement is thus provided by the positioning of elongated nanoparticles perpendicular to the cracks reinforcing the brittle polymer zone by CNT bridges. Resulting CNT pullout could be useful to characterize mechanical reinforcement in CNT-polymer composites (Hwang et al., 2004; Cooper et al., 2002).
TEM images of a MWNT crossing a hole in CNT polymer composites: the fiber pullout model.On the left side (from
Added to the development of tailored preparation procedures, studying CNT-based materials requires tools, techniques and methods that are able to relate the behavior at the molecular scale to the composite properties at a macroscopic scale (Wagner & Vaia, 2004). Beyond the experimental parameters or the nature of the CNT-polymer system, the mechanisms that take place at the CNT-polymer interface have to be better understood; they are indeed recognized to be the key for the CNT-based composite processing.
To summarize, significant progress has been achieved in the area of CNT processing. A range of new chemical treatments that are tailored in agreement with the desired application of the CNTs have demonstrated interesting results. Among them, covalently functionalized CNTs have been shown to improve both dispersion and polymer–CNT interaction. Avoiding damaging of the CNT structure upon chemical functionalization or purification procedure is still challenging. Further, the exact influence of the pre-treatments on the chemical and physical properties of the CNTs is difficult to assess. Regarding CNT-based composites, the prevailing problems of dispersion and stress transfer are not completely being overcome. However progress in this area has to be continued for the development of selective and innovative chemical treatments that will hopefully help for manufacturing of CNT-based devices and materials.
We would like to thank the Region Lorraine for financial support.
The well know endophytic plant growth promoting bacterium
The endophyte plant growth promoting bacteria:
Biochemical characteristics | 1. | 2. | 3. |
---|---|---|---|
Cell morphology: rods | − | − | − |
Morphology as rod on free carbon and nitrogen media | + | + | + |
Slime production | + | + | + |
Zeaxanthine dirhamnoside (yellow) | + | + | + |
Zeaxanthine (orange, pinkish) | − | − | − |
Motility under autotrophic growth conditions | −d | −d | −d |
Vitamins required for growth | + | + | + |
Sensitivity to chloramphenicol | − | − | − |
Under autotrophic growth at 35°C | + | + | + |
Utilization of hexoses | + | + | + |
Growth on nutrient broth | + | + | + |
Growth on glutamine as carbon source | + | + | + |
Growth on citrate | + | + | + |
Degradation of aromatic compounds | + | + | + |
Degradation of cyclohexene (and derivatives) | + | + | + |
Utilization of methanol | + | + | + |
Utilization of hydrocarbons | + | + | + |
Main biochemical characteristics among some species of the genus
1 =
Lime production in glucose.
Pale yellow indicating low concentration [6, 22, 23, 24, 25, 26].
Symbols and abbreviations: +, positive; (+), positive except for some unusual strains; −, negative; (−), negative except for some unusual strains; +/− not determined; TCA = tricarboxylic acid.
The phylogenetic position of
Photographs of
The most identifications of environmental isolates are done by 16S rRNA sequence analysis, in a first common identification step, diagnostic taxonomic properties are: (1) yellow, “fried egg” shaped colonies with several amounts of slime production under cultivation media specific conditions; (2) rods, some species have strong polymorphic, branched, twisted cell morphology growing on nutrient agar with larger amounts of polyphosphate granula, can lead to the false impression of a Gram-positive staining reaction; however all
Selective enrichment cultures. For isolation purposes, the use of free carbon and nitrogen agar medium as a selective medium is recommended for recovering
Phylogeny and taxonomy of
The known habitats of
In Japan was reported a survey of N2-fixing bacteria from roots of rice, with strains called group 2 were
Biofertilizer is key action of organic farming and a main element for the economy in general modern agricultural production on a world scale [55, 56, 58, 59]. The biofertilizers play an important role in improving the fertility of the soil [60, 61]. In addition, their application in soil improves the structure of the soil minimizes the sole application of chemical fertilizer. Grain yield and harvest index also increase with use of biofertilizers. Inoculation with
Biofertilizer products are usually based on the EPGPB or PGPM can be classified into three main types of microorganisms: arbuscular mycorrhizal fungi or AMF [72], plant growth promoting rhizobacteria or PGPR [73], and nitrogen fixing rhizobia and free nitrogen fixing bacteria for non-leguminous plant [74, 75] which are applied and approved as beneficial for domestic crops growth based in mineral nutritional, underline reported that PGPR are recommend worldwide as biofertilizers, contributing to maintain profitable yield without soil deterioration and preventing environmental pollution. Hence, with the potential contribution of the PGPR, to sustainable agriculture and forestry when pandemic condition of COVID 19 caused economic world depress [76, 77]. Sufficient densities of PGPR and/or EPGPB like
The increasing availability of N, P, and K is enhancing soil fertility, to improve antagonistic capacity of PGPR or EPGPB to biocontrol of plant pathogens agents [58] as well as the survival time in all types of soil [78]. Previous studies show that a biofertilizer prepared by mixing all types of PGPR with composts or carriers could enhance growth- promoting effects and biocontrol of plants [79].
In that sense EPGPB (likes PGPM or/and PGPR can be classified as biofertilizers when they sustainable options to plant nourishment and enrichment source that would useful for bioremediation and/or phytoremediation (double actions plants and biofertilizer) for soil contaminated by chemical agents [87, 88]. There for
A bioformulation is not effective until it does not have an impact in field conditions, market existence and reliability and cost effectiveness [93]. Production of bioformulation is not only dependent on the detailed knowledge of microbial as well as plant physiology, but a number of technological challenges are also involved such as fermentation process, formulation type, population of microbe, and delivery systems [94]. Barea [59] has published that in order to get better bioformulation for any domestic crops is important to understand the interaction among EPGPB or PGPM. To reproduce those microorganisms is important the chemical composition of broth media as well as the main and best conditions for each microorganisms need to get enough amount of them for bioformulation applying in open agriculture [95]. Including legal and ecological permission for safe crops production. A key quality of any bioformulation has to be water soluble to make sure a positive effect on any domestic crop Himel et al. [96] and Bateman [97] underline for those bioformulation which are applying in in aerosol based on a droplet size that is sufficient to inoculate seeds and plants with excellent results. For bioformulations applied foliarly, it is important to consider all environmental factors: solar radiation, high temperatures, ultraviolet light, etc. that limit the survival of beneficial plant microorganisms [98]. In this sense, the type of bioformulations must be appropriate to the form and vehicle that transport the beneficial plant microorganisms according to the recommended application directly to the soil, to the seeds or plants so that the forecast of the result favors agricultural production or control of some disease or pest [99]. Therefore, it is important research for the innovation of bioformulation suitable for agricultural crops [58] that comply with the quality and legality standards to satisfy the world market demand for safe food without risk of environmental damage [100]. A fundamental aspect for the world market of biological inoculants has been the necessary implementation of microbiological quality controls with reliable protocols that are endorsed by laws in the world that protect those farmers who, when applying them, have the confidence that they will have positive results in production. agriculture, due in part to the unfortunate experience of bioformulations without microbiological or legal quality that have caused a rejection of some sectors involved in sustainable agricultural production, an aspect that has not yet been resolved in the world [68]. In an integral sense that the biotechnology of the formulation of inoculants requires solid research for the best selection of microorganisms that promote plant growth, as well as the protocols of legal and ethical microbiological quality in the generation of bioformulations that give confidence to be used in the world for a sustainable and harmless agricultural production in harmony with the environment [85].
Related to phosphorus a key mineral for plant nutrition as phosphates normally applied to soil as fertilizer it is reported that concentration in average soils is about 0.05% (w/w) of which only 0.1% is available to plants [115]. There is evidence that the phosphate fertilizer applied as phosphate has a limited impact on plant nutrition, especially because, due to the solubilization constant (Ksp), of this phosphate anion is generally little available for plant roots [116]. It is calculated in the soil the concentration of phosphorus as phosphates is equal to or less than 0.02ppm, which drastically limits plant growth [117, 118]. In nature, the strategy that plants use for the absorption of the forms of phosphates necessary for plant metabolism are the solubilization actions of phosphates by genera and species of microorganisms that promote plant growth, such as mycorrhizae and bacteria that also mineralize organic compounds containing phosphates [119, 120]. In the last few years, the development of microbial inoculum containing phosphate-solubilizing microbes (PSM) gained attention of agriculturists [17].
Figure 3 shows the positive response of
Response of
Figure 4 shows the positive response of
Response of
In Figure 5, showed the response of
Positive response of
In Figure 6,
Response of
Figure 6 shows the effect of
The possible synthesis of phytohormons by
Response of
Table 2 shows the acid and alkaline phosphatase activity of
p-nitrophenol released (μg/ mL) | ||
---|---|---|
Saline solution (absolute control) | Acid | — |
Alkaline | — | |
Without inoculating stem | Acid | 0.45f** |
Alkaline | 0.13f | |
Without inoculating root | Acid | 1.49e |
Alkaline | 0.16f | |
Acid | 140.22c | |
Alkaline | 102.66d | |
Acid | 222.48a | |
Alkaline | 170.52b | |
Acid | 139.77c | |
Alkaline | 102.53d |
Activity of acid and alkaline phosphatases of
n = 3.
Values with different letter are stadistically distint according to ANOVA-Tukey (P < 0.05).
Figure 8 shows that fruit of
Fruit of
Figure 9 shows that
Response of
Figure 10 shows that
Effect of
The plant growth promoting endophytic bacteria well known as
The support of project 2.7 (2021-2022) of the Coordination of Scientific Research of the UMSNH, Morelia, Michoacan, Mexico is appreciated. As well as the project: “Field Test of a Living Biofertilizer for Crop Growth in Mexico” from Harvard University, Cambridge, Ma, USA with funding from the Rockefeller Foundation (2019–2022). To Jeaneth Caicedo Rengifo for her help working in this project. To Juan Luis Ignacio de la Cruz, MSc Blanca Celeste Saucedo Martinez and J Alberto Castro-Villaseñor for their technical support.
The authors declare no conflict of interest.
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Tunisia",slug:"role-of-the-ne-sw-hercynian-master-fault-systems-and-associated-lineaments-on-the-structuring-and-ev",totalDownloads:8150,totalCrossrefCites:17,totalDimensionsCites:26,abstract:null,book:{id:"2227",slug:"tectonics-recent-advances",title:"Tectonics",fullTitle:"Tectonics - Recent Advances"},signatures:"Fetheddine Melki, Taher Zouaghi, Mohamed Ben Chelbi, Mourad Bédir and Fouad Zargouni",authors:[{id:"39860",title:"Dr.",name:"Taher",middleName:null,surname:"Zouaghi",slug:"taher-zouaghi",fullName:"Taher Zouaghi"},{id:"147368",title:"Dr.",name:"Fetheddine",middleName:null,surname:"Melki",slug:"fetheddine-melki",fullName:"Fetheddine Melki"}]},{id:"30707",doi:"10.5772/29917",title:"Radon as Earthquake Precursor",slug:"radon-as-earthquake-precursor",totalDownloads:3763,totalCrossrefCites:11,totalDimensionsCites:20,abstract:null,book:{id:"2051",slug:"earthquake-research-and-analysis-statistical-studies-observations-and-planning",title:"Earthquake Research and 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Gökten",authors:[{id:"140276",title:"Dr.",name:"Akın",middleName:null,surname:"Kürçer",slug:"akin-kurcer",fullName:"Akın Kürçer"},{id:"144563",title:"Prof.",name:"Yaşar Ergun",middleName:null,surname:"Gökten",slug:"yasar-ergun-gokten",fullName:"Yaşar Ergun Gökten"}]}],mostDownloadedChaptersLast30Days:[{id:"64562",title:"Electrical Resistivity Tomography: A Subsurface-Imaging Technique",slug:"electrical-resistivity-tomography-a-subsurface-imaging-technique",totalDownloads:3141,totalCrossrefCites:7,totalDimensionsCites:9,abstract:"Electrical resistivity tomography (ERT) is a popular geophysical subsurface-imaging technique and widely applied to mineral prospecting, hydrological exploration, environmental investigation and civil engineering, as well as archaeological mapping. This chapter offers an overall review of technical aspects of ERT, which includes the fundamental theory of direct-current (DC) resistivity exploration, electrode arrays for data acquisition, numerical modelling methods and tomographic inversion algorithms. The section of fundamental theory shows basic formulae and principle of DC resistivity exploration. The section of electrode arrays summarises the previous study on all traditional-electrode arrays and recommends 4 electrode arrays for data acquisition of surface ERT and 3 electrode arrays for cross-hole ERT. The section of numerical modelling demonstrates an advanced version of finite-element method, called Gaussian quadrature grid approach, which is advantageous to a numerical simulation of ERT for complex geological models. The section of tomographic inversion presents the generalised standard conjugate gradient algorithms for both the l1- and l2-normed inversions. After that, some synthetic and real imaging examples are given to show the near-surface imaging capabilities of ERT.",book:{id:"8361",slug:"applied-geophysics-with-case-studies-on-environmental-exploration-and-engineering-geophysics",title:"Applied Geophysics with Case Studies on Environmental, Exploration and Engineering Geophysics",fullTitle:"Applied Geophysics with Case Studies on Environmental, Exploration and Engineering Geophysics"},signatures:"Bing Zhou",authors:null},{id:"37864",title:"Role of the NE-SW Hercynian Master Fault Systems and Associated Lineaments on the Structuring and Evolution of the Mesozoic and Cenozoic Basins of the Alpine Margin, Northern Tunisia",slug:"role-of-the-ne-sw-hercynian-master-fault-systems-and-associated-lineaments-on-the-structuring-and-ev",totalDownloads:8150,totalCrossrefCites:17,totalDimensionsCites:26,abstract:null,book:{id:"2227",slug:"tectonics-recent-advances",title:"Tectonics",fullTitle:"Tectonics - Recent Advances"},signatures:"Fetheddine Melki, Taher Zouaghi, Mohamed Ben Chelbi, Mourad Bédir and Fouad Zargouni",authors:[{id:"39860",title:"Dr.",name:"Taher",middleName:null,surname:"Zouaghi",slug:"taher-zouaghi",fullName:"Taher Zouaghi"},{id:"147368",title:"Dr.",name:"Fetheddine",middleName:null,surname:"Melki",slug:"fetheddine-melki",fullName:"Fetheddine Melki"}]},{id:"43258",title:"Speedy Techniques to Evaluate Seismic Site Effects in Particular Geomorphologic Conditions: Faults, Cavities, Landslides and Topographic Irregularities",slug:"speedy-techniques-to-evaluate-seismic-site-effects-in-particular-geomorphologic-conditions-faults-ca",totalDownloads:3013,totalCrossrefCites:8,totalDimensionsCites:19,abstract:null,book:{id:"3059",slug:"engineering-seismology-geotechnical-and-structural-earthquake-engineering",title:"Engineering Seismology, Geotechnical and Structural Earthquake Engineering",fullTitle:"Engineering Seismology, Geotechnical and Structural Earthquake Engineering"},signatures:"F. Panzera, G. Lombardo, S. D’Amico and P. Galea",authors:[{id:"52181",title:"Dr.",name:"Sebastiano",middleName:null,surname:"D'Amico",slug:"sebastiano-d'amico",fullName:"Sebastiano D'Amico"},{id:"58678",title:"Dr.",name:"Pauline",middleName:null,surname:"Galea",slug:"pauline-galea",fullName:"Pauline Galea"},{id:"167864",title:"Prof.",name:"Giuseppe",middleName:null,surname:"Lombardo",slug:"giuseppe-lombardo",fullName:"Giuseppe Lombardo"},{id:"167865",title:"Dr.",name:"Francesco",middleName:null,surname:"Panzera",slug:"francesco-panzera",fullName:"Francesco Panzera"}]},{id:"64060",title:"Advance Wave Modeling and Diffractions for High-Resolution Subsurface Seismic Imaging",slug:"advance-wave-modeling-and-diffractions-for-high-resolution-subsurface-seismic-imaging",totalDownloads:1156,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Seismic modeling and Imaging for the small-scale feature in a complex subsurface geology such as salt deposit, fracture reservoir, and Carbonate is not casual because of propagated wave affected by many objects once it hits the geologic structure in the subsurface. The principal goal of newly developed seismic modeling & imaging is to get a subsurface image of structural features with greatest sharpness or resolution. Using model dataset the Sigsbee and Marmousi, we illustrate the accuracy of conventional and advance wave modeling techniques. However, in conventional a Finite difference (FD) algorithm is used to generate the data and in advanced wave modeling, the low-rank (LR) approximation is used to acquire zero-offset configuration data. A field dataset from Malaysian basin is re-processed and imaged using diffraction imaging which shows an enhancement in structural interpretation. Furthermore, the results gained from the proposed modeling and imaging approach significantly enhance the bandwidth of the imaged data. Finally, a frequency spectrum shows a recovery of low-frequency from 0 to 60 Hz which is an optimal resolution of seismic imaging.",book:{id:"8361",slug:"applied-geophysics-with-case-studies-on-environmental-exploration-and-engineering-geophysics",title:"Applied Geophysics with Case Studies on Environmental, Exploration and Engineering Geophysics",fullTitle:"Applied Geophysics with Case Studies on Environmental, Exploration and Engineering Geophysics"},signatures:"Yasir Bashir and Deva Prasad Ghosh",authors:null},{id:"37858",title:"Geodynamic and Tectonostratigrafic Study of a Continental Rift: The Triassic Cuyana Basin, Argentina",slug:"geodynamic-and-tectonostratigrafic-study-of-a-continental-rift-the-triassic-cuyana-basin-argentina",totalDownloads:3419,totalCrossrefCites:0,totalDimensionsCites:14,abstract:null,book:{id:"2227",slug:"tectonics-recent-advances",title:"Tectonics",fullTitle:"Tectonics - Recent Advances"},signatures:"Silvia Patricia Barredo",authors:[{id:"147305",title:"Dr.",name:"Silvia",middleName:null,surname:"Barredo",slug:"silvia-barredo",fullName:"Silvia Barredo"}]}],onlineFirstChaptersFilter:{topicId:"654",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:287,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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While a daunting task, learning is facilitated by identifying common and effective signaling pathways mediated by a variety of factors employed by nature to preserve and sustain homeostatic life. \r\nAs a leading example, the cellular interaction between intracellular concentration of Ca+2 increases, and changes in plasma membrane potential is integral for coordinating blood flow, governing the exocytosis of neurotransmitters, and modulating gene expression and cell effector secretory functions. 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His primary area of interest is physiology and pathophysiology of the gastrointestinal (GI) tract, with the major focus on the mechanism of GI mucosal defense, protection, and ulcer healing. He was a postdoctoral NIH fellow at the University of California and the Gastroenterology VA Medical Center, Irvine, Long Beach, CA, USA, and at the Gastroenterology Clinics Erlangen-Nuremberg and Munster in Germany. He has published 290 original articles in some of the most prestigious scientific journals and seven book chapters on the pathophysiology of the GI tract, gastroprotection, ulcer healing, drug therapy of peptic ulcers, hormonal regulation of the gut, and inflammatory bowel disease.",institutionString:null,institution:{name:"Jagiellonian University",institutionURL:null,country:{name:"Poland"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"3",title:"Bacterial Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/3.jpg",isOpenForSubmission:!1,editor:null,editorTwo:null,editorThree:null},{id:"4",title:"Fungal Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",isOpenForSubmission:!0,editor:{id:"174134",title:"Dr.",name:"Yuping",middleName:null,surname:"Ran",slug:"yuping-ran",fullName:"Yuping Ran",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9d6QAC/Profile_Picture_1630330675373",biography:"Dr. Yuping Ran, Professor, Department of Dermatology, West China Hospital, Sichuan University, Chengdu, China. Completed the Course Medical Mycology, the Centraalbureau voor Schimmelcultures (CBS), Fungal Biodiversity Centre, Netherlands (2006). International Union of Microbiological Societies (IUMS) Fellow, and International Emerging Infectious Diseases (IEID) Fellow, Centers for Diseases Control and Prevention (CDC), Atlanta, USA. Diploma of Dermatological Scientist, Japanese Society for Investigative Dermatology. Ph.D. of Juntendo University, Japan. Bachelor’s and Master’s degree, Medicine, West China University of Medical Sciences. Chair of Sichuan Medical Association Dermatology Committee. General Secretary of The 19th Annual Meeting of Chinese Society of Dermatology and the Asia Pacific Society for Medical Mycology (2013). In charge of the Annual Medical Mycology Course over 20-years authorized by National Continue Medical Education Committee of China. Member of the board of directors of the Asia-Pacific Society for Medical Mycology (APSMM). Associate editor of Mycopathologia. Vice-chief of the editorial board of Chinses Journal of Mycology, China. Board Member and Chair of Mycology Group of Chinese Society of Dermatology.",institutionString:null,institution:{name:"Sichuan University",institutionURL:null,country:{name:"China"}}},editorTwo:null,editorThree:null},{id:"5",title:"Parasitic Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",isOpenForSubmission:!0,editor:{id:"67907",title:"Dr.",name:"Amidou",middleName:null,surname:"Samie",slug:"amidou-samie",fullName:"Amidou Samie",profilePictureURL:"https://mts.intechopen.com/storage/users/67907/images/system/67907.jpg",biography:"Dr. Amidou Samie is an Associate Professor of Microbiology at the University of Venda, in South Africa, where he graduated for his PhD in May 2008. He joined the Department of Microbiology the same year and has been giving lectures on topics covering parasitology, immunology, molecular biology and industrial microbiology. He is currently a rated researcher by the National Research Foundation of South Africa at category C2. He has published widely in the field of infectious diseases and has overseen several MSc’s and PhDs. His research activities mostly cover topics on infectious diseases from epidemiology to control. His particular interest lies in the study of intestinal protozoan parasites and opportunistic infections among HIV patients as well as the potential impact of childhood diarrhoea on growth and child development. He also conducts research on water-borne diseases and water quality and is involved in the evaluation of point-of-use water treatment technologies using silver and copper nanoparticles in collaboration with the University of Virginia, USA. He also studies the use of medicinal plants for the control of infectious diseases as well as antimicrobial drug resistance.",institutionString:null,institution:{name:"University of Venda",institutionURL:null,country:{name:"South Africa"}}},editorTwo:null,editorThree:null},{id:"6",title:"Viral Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",isOpenForSubmission:!0,editor:{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}},editorTwo:null,editorThree:null}]},overviewPageOFChapters:{paginationCount:4,paginationItems:[{id:"81821",title:"Pneumococcal Carriage in Jordanian Children and the Importance of Vaccination",doi:"10.5772/intechopen.104999",signatures:"Adnan Al-Lahham",slug:"pneumococcal-carriage-in-jordanian-children-and-the-importance-of-vaccination",totalDownloads:0,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}},{id:"81813",title:"Schistosomiasis: Discovery of New Molecules for Disease Treatment and Vaccine Development",doi:"10.5772/intechopen.104738",signatures:"Andressa Barban do Patrocinio",slug:"schistosomiasis-discovery-of-new-molecules-for-disease-treatment-and-vaccine-development",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"New Horizons for Schistosomiasis Research",coverURL:"https://cdn.intechopen.com/books/images_new/10829.jpg",subseries:{id:"5",title:"Parasitic Infectious Diseases"}}},{id:"81644",title:"Perspective Chapter: Ethics of Using Placebo Controlled Trials for Covid-19 Vaccine Development in Vulnerable Populations",doi:"10.5772/intechopen.104776",signatures:"Lesley Burgess, Jurie Jordaan and Matthew Wilson",slug:"perspective-chapter-ethics-of-using-placebo-controlled-trials-for-covid-19-vaccine-development-in-vu",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"SARS-CoV-2 Variants - Two Years After",coverURL:"https://cdn.intechopen.com/books/images_new/11573.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"80546",title:"Streptococcal Skin and Skin-Structure Infections",doi:"10.5772/intechopen.102894",signatures:"Alwyn Rapose",slug:"streptococcal-skin-and-skin-structure-infections",totalDownloads:48,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}}]},overviewPagePublishedBooks:{paginationCount:13,paginationItems:[{type:"book",id:"6667",title:"Influenza",subtitle:"Therapeutics and Challenges",coverURL:"https://cdn.intechopen.com/books/images_new/6667.jpg",slug:"influenza-therapeutics-and-challenges",publishedDate:"September 19th 2018",editedByType:"Edited by",bookSignature:"Shailendra K. 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Saxena",hash:"d92a4085627bab25ddc7942fbf44cf05",volumeInSeries:2,fullTitle:"Current Perspectives in Human Papillomavirus",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Bacterial Infectious Diseases",value:3,count:2},{group:"subseries",caption:"Parasitic Infectious Diseases",value:5,count:4},{group:"subseries",caption:"Viral Infectious Diseases",value:6,count:7}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:2},{group:"publicationYear",caption:"2021",value:2021,count:4},{group:"publicationYear",caption:"2020",value:2020,count:3},{group:"publicationYear",caption:"2019",value:2019,count:3},{group:"publicationYear",caption:"2018",value:2018,count:1}],authors:{paginationCount:230,paginationItems:[{id:"61139",title:"Dr.",name:"Sergey",middleName:null,surname:"Tkachev",slug:"sergey-tkachev",fullName:"Sergey Tkachev",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61139/images/system/61139.png",biography:"Dr. Sergey Tkachev is a senior research scientist at the Institute of Fundamental Medicine and Biology, Kazan Federal University, Russia, and at the Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. He received his Ph.D. in Molecular Biology with his thesis “Genetic variability of the tick-borne encephalitis virus in natural foci of Novosibirsk city and its suburbs.” His primary field is molecular virology with research emphasis on vector-borne viruses, especially tick-borne encephalitis virus, Kemerovo virus and Omsk hemorrhagic fever virus, rabies virus, molecular genetics, biology, and epidemiology of virus pathogens.",institutionString:"Russian Academy of Sciences",institution:{name:"Russian Academy of Sciences",country:{name:"Russia"}}},{id:"310962",title:"Dr.",name:"Amlan",middleName:"Kumar",surname:"Patra",slug:"amlan-patra",fullName:"Amlan Patra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/310962/images/system/310962.jpg",biography:"Amlan K. Patra, FRSB, obtained a Ph.D. in Animal Nutrition from Indian Veterinary Research Institute, India, in 2002. He is currently an associate professor at West Bengal University of Animal and Fishery Sciences. He has more than twenty years of research and teaching experience. He held previous positions at the American Institute for Goat Research, The Ohio State University, Columbus, USA, and Free University of Berlin, Germany. His research focuses on animal nutrition, particularly ruminants and poultry nutrition, gastrointestinal electrophysiology, meta-analysis and modeling in nutrition, and livestock–environment interaction. He has authored around 175 articles in journals, book chapters, and proceedings. Dr. Patra serves on the editorial boards of several reputed journals.",institutionString:null,institution:{name:"West Bengal University of Animal and Fishery Sciences",country:{name:"India"}}},{id:"53998",title:"Prof.",name:"László",middleName:null,surname:"Babinszky",slug:"laszlo-babinszky",fullName:"László Babinszky",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/53998/images/system/53998.png",biography:"László Babinszky is Professor Emeritus, Department of Animal Nutrition Physiology, University of Debrecen, Hungary. He has also worked in the Department of Animal Nutrition, University of Wageningen, Netherlands; the Institute for Livestock Feeding and Nutrition (IVVO), Lelystad, Netherlands; the Agricultural University of Vienna (BOKU); the Institute for Animal Breeding and Nutrition, Austria; and the Oscar Kellner Research Institute for Animal Nutrition, Rostock, Germany. In 1992, Dr. Babinszky obtained a Ph.D. in Animal Nutrition from the University of Wageningen. His main research areas are swine and poultry nutrition. He has authored more than 300 publications (papers, book chapters) and edited four books and fourteen international conference proceedings.",institutionString:"University of Debrecen",institution:{name:"University of Debrecen",country:{name:"Hungary"}}},{id:"201830",title:"Dr.",name:"Fernando",middleName:"Sanchez",surname:"Davila",slug:"fernando-davila",fullName:"Fernando Davila",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/201830/images/5017_n.jpg",biography:"I am a professor at UANL since 1988. My research lines are the development of reproductive techniques in small ruminants. We also conducted research on sexual and social behavior in males.\nI am Mexican and study my professional career as an engineer in agriculture and animal science at UANL. Then take a masters degree in science in Germany (Animal breeding). Take a doctorate in animal science at the UANL.",institutionString:null,institution:{name:"Universidad Autónoma de Nuevo León",country:{name:"Mexico"}}},{id:"309250",title:"Dr.",name:"Miguel",middleName:null,surname:"Quaresma",slug:"miguel-quaresma",fullName:"Miguel Quaresma",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/309250/images/9059_n.jpg",biography:"Miguel Nuno Pinheiro Quaresma was born on May 26, 1974 in Dili, Timor Island. He is married with two children: a boy and a girl, and he is a resident in Vila Real, Portugal. He graduated in Veterinary Medicine in August 1998 and obtained his Ph.D. degree in Veterinary Sciences -Clinical Area in February 2015, both from the University of Trás-os-Montes e Alto Douro. He is currently enrolled in the Alternative Residency of the European College of Animal Reproduction. He works as a Senior Clinician at the Veterinary Teaching Hospital of UTAD (HVUTAD) with a role in clinical activity in the area of livestock and equine species as well as to support teaching and research in related areas. He teaches as an Invited Professor in Reproduction Medicine I and II of the Master\\'s in Veterinary Medicine degree at UTAD. Currently, he holds the position of Chairman of the Portuguese Buiatrics Association. He is a member of the Consultive Group on Production Animals of the OMV. He has 19 publications in indexed international journals (ISIS), as well as over 60 publications and oral presentations in both Portuguese and international journals and congresses.",institutionString:"University of Trás-os-Montes and Alto Douro",institution:{name:"University of Trás-os-Montes and Alto Douro",country:{name:"Portugal"}}},{id:"38652",title:"Dr.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRiFPQA0/Profile_Picture_1614601496313",biography:"Rita Payan Carreira earned her Veterinary Degree from the Faculty of Veterinary Medicine in Lisbon, Portugal, in 1985. She obtained her Ph.D. in Veterinary Sciences from the University of Trás-os-Montes e Alto Douro, Portugal. After almost 32 years of teaching at the University of Trás-os-Montes and Alto Douro, she recently moved to the University of Évora, Department of Veterinary Medicine, where she teaches in the field of Animal Reproduction and Clinics. Her primary research areas include the molecular markers of the endometrial cycle and the embryo–maternal interaction, including oxidative stress and the reproductive physiology and disorders of sexual development, besides the molecular determinants of male and female fertility. She often supervises students preparing their master's or doctoral theses. She is also a frequent referee for various journals.",institutionString:null,institution:{name:"University of Évora",country:{name:"Portugal"}}},{id:"283019",title:"Dr.",name:"Oudessa",middleName:null,surname:"Kerro Dego",slug:"oudessa-kerro-dego",fullName:"Oudessa Kerro Dego",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/283019/images/system/283019.png",biography:"Dr. Kerro Dego is a veterinary microbiologist with training in veterinary medicine, microbiology, and anatomic pathology. Dr. Kerro Dego is an assistant professor of dairy health in the department of animal science, the University of Tennessee, Institute of Agriculture, Knoxville, Tennessee. He received his D.V.M. (1997), M.S. (2002), and Ph.D. (2008) degrees in Veterinary Medicine, Animal Pathology and Veterinary Microbiology from College of Veterinary Medicine, Addis Ababa University, Ethiopia; College of Veterinary Medicine, Utrecht University, the Netherlands and Western College of Veterinary Medicine, University of Saskatchewan, Canada respectively. He did his Postdoctoral training in microbial pathogenesis (2009 - 2015) in the Department of Animal Science, the University of Tennessee, Institute of Agriculture, Knoxville, Tennessee. Dr. Kerro Dego’s research focuses on the prevention and control of infectious diseases of farm animals, particularly mastitis, improving dairy food safety, and mitigation of antimicrobial resistance. Dr. Kerro Dego has extensive experience in studying the pathogenesis of bacterial infections, identification of virulence factors, and vaccine development and efficacy testing against major bacterial mastitis pathogens. Dr. Kerro Dego conducted numerous controlled experimental and field vaccine efficacy studies, vaccination, and evaluation of immunological responses in several species of animals, including rodents (mice) and large animals (bovine and ovine).",institutionString:"University of Tennessee at Knoxville",institution:{name:"University of Tennessee at Knoxville",country:{name:"United States of America"}}},{id:"251314",title:"Dr.",name:"Juan Carlos",middleName:null,surname:"Gardón",slug:"juan-carlos-gardon",fullName:"Juan Carlos Gardón",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/251314/images/system/251314.jpeg",biography:"Juan Carlos Gardón Poggi received University degree from the Faculty of Agrarian Science in Argentina, in 1983. Also he received Masters Degree and PhD from Córdoba University, Spain. He is currently a Professor at the Catholic University of Valencia San Vicente Mártir, at the Department of Medicine and Animal Surgery. He teaches diverse courses in the field of Animal Reproduction and he is the Director of the Veterinary Farm. He also participates in academic postgraduate activities at the Veterinary Faculty of Murcia University, Spain. His research areas include animal physiology, physiology and biotechnology of reproduction either in males or females, the study of gametes under in vitro conditions and the use of ultrasound as a complement to physiological studies and development of applied biotechnologies. Routinely, he supervises students preparing their doctoral, master thesis or final degree projects.",institutionString:"Catholic University of Valencia San Vicente Mártir, Spain",institution:null},{id:"125292",title:"Dr.",name:"Katy",middleName:null,surname:"Satué Ambrojo",slug:"katy-satue-ambrojo",fullName:"Katy Satué Ambrojo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/125292/images/system/125292.jpeg",biography:"Katy Satué Ambrojo received her Veterinary Medicine degree, Master degree in Equine Technology and doctorate in Veterinary Medicine from the Faculty of Veterinary, CEU-Cardenal Herrera University in Valencia, Spain. She is a Full Professor at the Department of Medicine and Animal Surgery at the same University. She developed her research activity in the field of Endocrinology, Hematology, Biochemistry and Immunology of horses. She is a scientific reviewer of several international journals : American Journal of Obstetrics and Gynecology, Comparative Clinical Pathology, Veterinary Clinical Pathology, Journal of Equine Veterinary Science, Reproduction in Domestic Animals, Research Veterinary Science, Brazilian Journal of Medical and Biological Research, Livestock Production Science and Theriogenology. Since 2014, she has been the Head of the Clinical Analysis Laboratory of the Hospital Clínico Veterinario from the Faculty of Veterinary, CEU-Cardenal Herrera University.",institutionString:"CEU-Cardenal Herrera University",institution:{name:"CEU Cardinal Herrera University",country:{name:"Spain"}}},{id:"309529",title:"Dr.",name:"Albert",middleName:null,surname:"Rizvanov",slug:"albert-rizvanov",fullName:"Albert Rizvanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/309529/images/9189_n.jpg",biography:'Albert A. Rizvanov is a Professor and Director of the Center for Precision and Regenerative Medicine at the Institute of Fundamental Medicine and Biology, Kazan Federal University (KFU), Russia. He is the Head of the Center of Excellence “Regenerative Medicine” and Vice-Director of Strategic Academic Unit \\"Translational 7P Medicine\\". Albert completed his Ph.D. at the University of Nevada, Reno, USA and Dr.Sci. at KFU. He is a corresponding member of the Tatarstan Academy of Sciences, Russian Federation. Albert is an author of more than 300 peer-reviewed journal articles and 22 patents. He has supervised 11 Ph.D. and 2 Dr.Sci. dissertations. Albert is the Head of the Dissertation Committee on Biochemistry, Microbiology, and Genetics at KFU.\nORCID https://orcid.org/0000-0002-9427-5739\nWebsite https://kpfu.ru/Albert.Rizvanov?p_lang=2',institutionString:"Kazan Federal University",institution:{name:"Kazan Federal University",country:{name:"Russia"}}},{id:"210551",title:"Dr.",name:"Arbab",middleName:null,surname:"Sikandar",slug:"arbab-sikandar",fullName:"Arbab Sikandar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210551/images/system/210551.jpg",biography:"Dr. Arbab Sikandar, PhD, M. Phil, DVM was born on April 05, 1981. He is currently working at the College of Veterinary & Animal Sciences as an Assistant Professor. He previously worked as a lecturer at the same University. \nHe is a Member/Secretory of Ethics committee (No. CVAS-9377 dated 18-04-18), Member of the QEC committee CVAS, Jhang (Regr/Gen/69/873, dated 26-10-2017), Member, Board of studies of Department of Basic Sciences (No. CVAS. 2851 Dated. 12-04-13, and No. CVAS, 9024 dated 20/11/17), Member of Academic Committee, CVAS, Jhang (No. CVAS/2004, Dated, 25-08-12), Member of the technical committee (No. CVAS/ 4085, dated 20,03, 2010 till 2016).\n\nDr. Arbab Sikandar contributed in five days hands-on-training on Histopathology at the Department of Pathology, UVAS from 12-16 June 2017. He received a Certificate of appreciation for contributions for Popularization of Science and Technology in the Society on 17-11-15. He was the resource person in the lecture series- ‘scientific writing’ at the Department of Anatomy and Histology, UVAS, Lahore on 29th October 2015. He won a full fellowship as a principal candidate for the year 2015 in the field of Agriculture, EICA, Egypt with ref. to the Notification No. 12(11) ACS/Egypt/2014 from 10 July 2015 to 25th September 2015.; he received a grant of Rs. 55000/- as research incentives from Director, Advanced Studies and Research, UVAS, Lahore upon publications of research papers in IF Journals (DR/215, dated 19-5-2014.. He obtained his PhD by winning a HEC Pakistan indigenous Scholarship, ‘Ph.D. fellowship for 5000 scholars – Phase II’ (2av1-147), 17-6/HEC/HRD/IS-II/12, November 15, 2012. \n\nDr. Sikandar is a member of numerous societies: Registered Veterinary Medical Practitioner (life member) and Registered Veterinary Medical Faculty of Pakistan Veterinary Medical Council. The Registration code of PVMC is RVMP/4298 and RVMF/ 0102.; Life member of the University of Veterinary and Animal Sciences, Lahore, Alumni Association with S# 664, dated: 6-4-12. ; Member 'Vets Care Organization Pakistan” with Reference No. VCO-605-149, dated 05-04-06. :Member 'Vet Crescent” (Society of Animal Health and Production), UVAS, Lahore.",institutionString:"University of Veterinary & Animal Science",institution:{name:"University of Veterinary and Animal Sciences",country:{name:"Pakistan"}}},{id:"311663",title:"Dr.",name:"Prasanna",middleName:null,surname:"Pal",slug:"prasanna-pal",fullName:"Prasanna Pal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311663/images/13261_n.jpg",biography:null,institutionString:null,institution:{name:"National Dairy Research Institute",country:{name:"India"}}},{id:"202192",title:"Dr.",name:"Catrin",middleName:null,surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",biography:"Catrin Rutland is an Associate Professor of Anatomy and Developmental Genetics at the University of Nottingham, UK. She obtained a BSc from the University of Derby, England, a master’s degree from Technische Universität München, Germany, and a Ph.D. from the University of Nottingham. She undertook a post-doctoral research fellowship in the School of Medicine before accepting tenure in Veterinary Medicine and Science. Dr. Rutland also obtained an MMedSci (Medical Education) and a Postgraduate Certificate in Higher Education (PGCHE). She is the author of more than sixty peer-reviewed journal articles, twelve books/book chapters, and more than 100 research abstracts in cardiovascular biology and oncology. She is a board member of the European Association of Veterinary Anatomists, Fellow of the Anatomical Society, and Senior Fellow of the Higher Education Academy. Dr. Rutland has also written popular science books for the public. https://orcid.org/0000-0002-2009-4898. www.nottingham.ac.uk/vet/people/catrin.rutland",institutionString:null,institution:{name:"University of Nottingham",country:{name:"United Kingdom"}}},{id:"283315",title:"Prof.",name:"Samir",middleName:null,surname:"El-Gendy",slug:"samir-el-gendy",fullName:"Samir El-Gendy",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRduYQAS/Profile_Picture_1606215849748",biography:"Samir El-Gendy is a Professor of anatomy and embryology at the faculty of veterinary medicine, Alexandria University, Egypt. Samir obtained his PhD in veterinary science in 2007 from the faculty of veterinary medicine, Alexandria University and has been a professor since 2017. Samir is an author on 24 articles at Scopus and 12 articles within local journals and 2 books/book chapters. His research focuses on applied anatomy, imaging techniques and computed tomography. Samir worked as a member of different local projects on E-learning and he is a board member of the African Association of Veterinary Anatomists and of anatomy societies and as an associated author at local and international journals. Orcid: https://orcid.org/0000-0002-6180-389X",institutionString:null,institution:{name:"Alexandria University",country:{name:"Egypt"}}},{id:"246149",title:"Dr.",name:"Valentina",middleName:null,surname:"Kubale",slug:"valentina-kubale",fullName:"Valentina Kubale",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246149/images/system/246149.jpg",biography:"Valentina Kubale is Associate Professor of Veterinary Medicine at the Veterinary Faculty, University of Ljubljana, Slovenia. Since graduating from the Veterinary faculty she obtained her PhD in 2007, performed collaboration with the Department of Pharmacology, University of Copenhagen, Denmark. She continued as a post-doctoral fellow at the University of Copenhagen with a Lundbeck foundation fellowship. She is the editor of three books and author/coauthor of 23 articles in peer-reviewed scientific journals, 16 book chapters, and 68 communications at scientific congresses. Since 2008 she has been the Editor Assistant for the Slovenian Veterinary Research journal. She is a member of Slovenian Biochemical Society, The Endocrine Society, European Association of Veterinary Anatomists and Society for Laboratory Animals, where she is board member.",institutionString:"University of Ljubljana",institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"258334",title:"Dr.",name:"Carlos Eduardo",middleName:null,surname:"Fonseca-Alves",slug:"carlos-eduardo-fonseca-alves",fullName:"Carlos Eduardo Fonseca-Alves",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/258334/images/system/258334.jpg",biography:"Dr. Fonseca-Alves earned his DVM from Federal University of Goias – UFG in 2008. He completed an internship in small animal internal medicine at UPIS university in 2011, earned his MSc in 2013 and PhD in 2015 both in Veterinary Medicine at Sao Paulo State University – UNESP. Dr. Fonseca-Alves currently serves as an Assistant Professor at Paulista University – UNIP teaching small animal internal medicine.",institutionString:null,institution:{name:"Universidade Paulista",country:{name:"Brazil"}}},{id:"245306",title:"Dr.",name:"María Luz",middleName:null,surname:"Garcia Pardo",slug:"maria-luz-garcia-pardo",fullName:"María Luz Garcia Pardo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/245306/images/system/245306.png",biography:"María de la Luz García Pardo is an agricultural engineer from Universitat Politècnica de València, Spain. She has a Ph.D. in Animal Genetics. Currently, she is a lecturer at the Agrofood Technology Department of Miguel Hernández University, Spain. Her research is focused on genetics and reproduction in rabbits. The major goal of her research is the genetics of litter size through novel methods such as selection by the environmental sensibility of litter size, with forays into the field of animal welfare by analysing the impact on the susceptibility to diseases and stress of the does. Details of her publications can be found at https://orcid.org/0000-0001-9504-8290.",institutionString:null,institution:{name:"Miguel Hernandez University",country:{name:"Spain"}}},{id:"41319",title:"Prof.",name:"Lung-Kwang",middleName:null,surname:"Pan",slug:"lung-kwang-pan",fullName:"Lung-Kwang Pan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41319/images/84_n.jpg",biography:null,institutionString:null,institution:null},{id:"201721",title:"Dr.",name:"Beatrice",middleName:null,surname:"Funiciello",slug:"beatrice-funiciello",fullName:"Beatrice Funiciello",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/201721/images/11089_n.jpg",biography:"Graduated from the University of Milan in 2011, my post-graduate education included CertAVP modules mainly on equines (dermatology and internal medicine) and a few on small animal (dermatology and anaesthesia) at the University of Liverpool. After a general CertAVP (2015) I gained the designated Certificate in Veterinary Dermatology (2017) after taking the synoptic examination and then applied for the RCVS ADvanced Practitioner status. After that, I completed the Postgraduate Diploma in Veterinary Professional Studies at the University of Liverpool (2018). My main area of work is cross-species veterinary dermatology.",institutionString:null,institution:null},{id:"291226",title:"Dr.",name:"Monica",middleName:null,surname:"Cassel",slug:"monica-cassel",fullName:"Monica Cassel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/291226/images/8232_n.jpg",biography:'Degree in Biological Sciences at the Federal University of Mato Grosso with scholarship for Scientific Initiation by FAPEMAT (2008/1) and CNPq (2008/2-2009/2): Project \\"Histological evidence of reproductive activity in lizards of the Manso region, Chapada dos Guimarães, Mato Grosso, Brazil\\". Master\\\'s degree in Ecology and Biodiversity Conservation at Federal University of Mato Grosso with a scholarship by CAPES/REUNI program: Project \\"Reproductive biology of Melanorivulus punctatus\\". PhD\\\'s degree in Science (Cell and Tissue Biology Area) \n at University of Sao Paulo with scholarship granted by FAPESP; Project \\"Development of morphofunctional changes in ovary of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae)\\". She has experience in Reproduction of vertebrates and Morphology, with emphasis in Cellular Biology and Histology. 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