Chapters authored
Morphological, Biochemical, and Molecular Characterization of Orange-Fleshed Sweet Potato (Ipomoea batatas [L.] Lam) Germplasms
The sweet potato is considered as an excellent source of β-carotene and anthocyanins and has a considerable value in the functional food market. In this report, 21 sweet potato (Ipomoea batatas [L.] Lam) germplasms were evaluated for genetic diversity using morphological and biochemical and molecular markers. Ten morphological traits were studied, and the mean squares due to germplasm were highly significant for storage root number per plant, individual root weight, storage root (fresh) per plant, storage root (dry) per plant, storage root yield, and storage root length. UPGMA cluster analysis based on morphological traits separated the germplasm into three groups. The similarity coefficient ranged from 0.00 to 0.50 with an average of 0.176. Biochemical analysis, viz. total phenol and antioxidant, was performed to find out superior genotype at biochemical level under given conditions. Maximum total phenol was observed in the genotype “V-12” (1.39 mg), whereas maximum total antioxidant was observed in “Samrat” (0.30 mg). RAPD analysis was carried out, and out of 15 RAPD primers, 10 primers produced 96 reproducible and polymorphic bands. UPGMA cluster analysis based on RAPD data also separated the genotypes into three clusters. The results of the present study can be used for sweet potato crop improvement through molecular breeding and marker-assisted selection for desired traits in future.
Part of the book: Genetic Diversity in Plant Species