GC–MS-based metabolomic profiling of different strains of basidiomycetes Lignomyces vetlinianus, Daedaleopsis tricolor and Sparassis crispa were studied. On different stages of growth in the methanol extracts of mycelium the different compounds including amino acids, organic acid of TCA cycle, sugars, fatty acids, sugar alcohols, and sugar acids were detected. Changes in the metabolite network occurring with age of the mycelium of L. vetlinianus and D. tricolor are discussed. The exponential phase of mycelium growth is characterized by pronounced differences during of growth, which manifests itself both in the analysis of specific compounds and in the modeling of the statistical model of the metabolic network. The metabolomic network in the stationary growth phase is less susceptible to changes over time, and is also characterized by a lower dispersion of samples from one aging group. For some compounds, including biotechnologically significant ones, targeted analysis by GC–MS was performed. 4, 6-dimethoxy-phthalide (4, 6-dimetoxy-1 (3H) -isobenzofuranone) was isolated from the mycelium of Lignomyces vetlinianus, accumulating in the mycelium in the form of large aggregates. The accumulation of sparassol and other orsellinic acid derivatives in Sparassis crispa culture under various conditions is described.
Part of the book: Metabolomics