\r\n\tManagement of these disorders requires good clinical evaluation, diagnostic tests, appropriate therapy and huge healthcare cost. Sometimes multiple specialties (gastroenterologists, \r\n\tgastrointestinal motility specialists, otolaryngologists, surgeons, speech therapists, medical oncologists and radiation oncologists) are involved in the management of dysphonia and dysphagia. In the recent years, there have been many updates in the management of these disorders. This book will discuss systematically the different etiologies and management of dysphonia, maxillofacial, oropharyngeal and esophageal dysphagia. This book will be a good \r\n\tguide to the practicing physicians for the management of voice and swallowing disorders.
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1. Introduction
A chromatographic method combined with mass spectrometry is the key technique used in analysis of trace-level compounds present in complex matrices. However, success depends on the enrichment and extraction of target analytes from the matrix. This study investigated the development of a microextraction technique for use in the analysis of pesticides present at trace levels in field water samples and vegetable matter.
The most common extraction techniques used in environmental analysis are liquid–liquid extraction (LLE) [1, 2] and solid-phase extraction (SPE) [3, 4], both of which are time-consuming and use large volumes of samples. As a result, much attention is being paid to the development of more efficient and environmentally friendly extraction techniques, such as solid-phase microextraction (SPME) and liquid-phase microextraction (LPME). SPME, a solvent- free technique, was developed by Arthur and Pawlizyn [5]; however, the fiber is expensive and fragile, and the problem of sample carry-over cannot always be eliminated [4, 6-8]. LPME, which was introduced by Cantwell and co-workers [9], minimizes solvent usage and solvent variation; this technique is of interest to many analysts. Single-droplet microextraction (SDME) [10-13], solvent-bar microextraction (SBME) [14, 15] and hollow fiber-LPME (HF-LPME) [16-19] have been developed in the past few years but longer extraction times are required to obtain good extraction efficiencies. Efforts to overcome these limitations led to the development of dispersive liquid–liquid microextraction (DLLME) with the advantages of short extraction times, ease of operation, and small amounts of solvents used [20-22]. In DLLME, a water-immiscible extraction solvent, which is dissolved in a water-miscible dispersive solvent, is introduced rapidly by syringe into an aqueous sample in a conical centrifuge tube. A cloudy mixture containing fine droplets of the extraction solvent dispersed entirely in the aqueous phase is formed. The organic phase drop, which precipitates in the bottom of the tube after centrifugation, is injected into an injection port of gas chromatography (GC) or high-performance liquid chromatography (HPLC) for further analysis.
Lately, a novel microextraction technique called ultrasound-assisted emulsification microextraction (USAEME) was developed by Garcia-Jares and co-workers [23]. In USAEME, a very small volume of water-immiscible extraction solvent is mixed with an aqueous sample solution by ultrasound-assisted emulsification to form fine droplets for extracting analytes, obviating the need of a dispersive solvent in DLLME [24]. The ultrasound-assisted emulsification is carried out at 25ºC for 10 min [23, 25] or for 9 min [26]. Recently, a few reports indicated that the use of manual shaking before ultrasound-assisted emulsification enhanced extraction efficiency. Fuh and co-workers used ultrasound with occasional manual shaking to generate a cloudy suspension [26]. The approach reported by Fontana and co-workers mixed honey samples with extraction solvent, Triton X-114, and used manual shaking to generate a homogeneous solution [27]. The work performed by Huang et al. showed that manual shaking for 10 s before ultrasound-assisted emulsification enhances the extraction efficiency of organochlorine pesticides (OCPs) by >100% in aqueous samples [28].
In this study, a new technique, manual shaking-enhanced, ultrasound-assisted emulsification microextraction (MS-USAEME) has been developed. Carbamate pesticides were chosen as the target analytes to evaluate the performance of the proposed method. Carbamate pesticides have been used for decades in many countries to increase agricultural production, are acetylcholinesterase inhibitors that allow acetylcholine to accumulate in the human body, resulting in health problems. Their residues can appear in fruit and vegetables, and are usually distributed in aqueous environments by leaching and runoff from soil into ground and surface water because of their high solubilities in water [29-31]. In order to detect trace amounts of the pesticides, the effects of changes of various experimental parameters, such as the nature and volume of the extraction solvent, duration of ultrasound emulsification, the effect of manual shaking and the addition of salt, were investigated and optimized. The MS-USAEME technique is simple and efficient. The objective of this study was to investigate the use of MS-USAEME for the extraction of carbamate pesticides from field water and vegetable matter.
2. Experimental
2.1. Reagents and materials
The carbamates, propoxur (99.8% purity) and pirimicarb (99% purity), were purchased from Fluka (Steinheim, Germany). Carbaryl, (99.8% purity) and methiocarb (99.5% purity) were purchased from Chem Service (West Chester, PA, USA). Carbofuran (98% purity) was purchased from Aldrich (Saint Louis, MO, USA). Stock 1 g L-1 solutions of each pesticide were prepared in methanol (HPLC-grade) and stored at 4 ºC. Mixed working standard solutions were prepared daily with deionized (DI) water purified with a Milli-Q system (Millipore, Bedford, MA, USA).
1-Octanol (99% purity), 1-nonanol (98% purity), 1-decanol (98% purity) and sodium chloride (NaCl, 99.5% purity) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). 1-Undecanol was purchased from TCI (Tokyo, Japan). HPLC-grade methanol was purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA).
2.2. Sample preparation
Field water samples (from Cyonglin, Hsinchu, Taiwan) were passed through a 0.45 µm pore size membrane filter (Millipore, Bedford, MA, USA) and stored at 4 ºC.
A sample of lettuce (Lactuca sativa) (from Siluo Township, Yunlin County, Taiwan) was chopped in a food chopper, then 2 L of deionized (DI) water was added per gram of chopped lettuce and the mixture homogenized in a food homogenizer. A 15 mL sample of the homogenized lettuce was centrifuged at 1398 × g for 10 min in a benchtop centrifuge; the supernatant liquid was then passed through a 0.45 µm pore size membrane (Millipore, Bedford, MA, USA) and stored at 4 ºC.
2.3. Instrumentation
Analysis was performed on an Agilent gas chromatograph (6850 series, Wilmington, DE, USA) equipped with a split/splitless injector and coupled with an Agilent mass spectrometer (5978B series). A DB-5 MS UI fused silica capillary column (30 m × 0.25 mm I.D., 0.25 μm film thickness; J & W Scientific, Folsom, CA, USA) was used for separation of the analytes. Initially the column temperature was held at 140 ºC for 1 min, then ramped to 270 ºC at 20 ºC min-1 and kept at that temperature for 3.5 min. The carrier gas was helium (purity 99.9995%) that had been further purified by passage through an Agilent helium gas purifier (model RMSH-2) and the flow rate was 1.0 mL min-1. The inlet was operated at 300 ºC and was used in the pulsed splitless mode. Ionization was operated in the electron impact (EI) mode at 70 eV. The temperature of the ion source was 230 ºC and the temperature of the quadrupole mass filter was 150 ºC. The MS was operated in full scan mode and a mass range of m/z 50–250 was scanned to confirm the retention times of the analytes. The selected ion monitoring (SIM) mode was used for the determination of the target compounds. Two selection ions were used for quantitation, and scan start times of the compounds were studied by gas chromatography-mass spectrometry (GC-MS): m/z 110, 152 (Propoxur); m/z 103, 164 (Carbofuran); m/z 115, 144 (Carbaryl); m/z 109, 153 (Methiocarb); m/z 166, 238 (Pirimicarb). The mass spectrometer was turned ON at 3.5 min and OFF after 11 min, to avoid filament breaking.
The ultrasonic water bath was obtained from Branson Ultrasonics (Danbury, CT, USA). The ultrasound frequency and power were 42 Hz and 100 W, respectively.
2.4. Analytical procedure
A 10 mL sample of DI water was placed in a glass centrifuge tube and spiked with five carbamate analytes at the required concentrations; 2.5 g of NaCl was added and dissolved completely. A 10 μL portion of 1-octanol (the extraction solvent) was added and the tube was manually shaken gently for 10 s, and then immersed in an ultrasonic water bath. The levels of water bath and solution in the tube were both the same. During ultrasonication for 3 min at room temperature, the bath and the tube contents became cloudy due to the dispersion of fine droplets of 1-octanol within the aqueous bulk. After centrifugation at 1398 × g for 3 min in a benchtop centrifuge, the extraction solvent floated on the aqueous phase; the floating extraction phase (3 μL) was collected with a 25 μL microsyringe and transferred to a microtube. One μL of this extractant was injected into the GC for analysis (Figure 1). The above procedure was applied to field water and to the supernatant liquid from the lettuce samples mentioned in section 2.2.
Figure 1.
The analytical procedure.
3. Results and discussion
3.1. Optimization of the experimental conditions
In order to obtain the most effective extraction of carbamate pesticides, it is important to determine the optimum conditions for the analysis. The variable parameters include the nature and volume of the extraction solvent, the ultrasonication time, the ionic strength and the effect of manual shaking. The behavior of five carbamate pesticides was studied under various extraction conditions. The calculation of enrichment factor (EF) for this method was (concentration of analyte in the floating phase, Corg) divided by (initial concentration of analyte in the aqueous sample, C0).
EF =Corg/C0
3.1.1. Selection of extraction solvent
The selection of an extraction solvent is the most important experimental parameter in this method. An appropriate extraction solvent must have: (1) low toxicity, (2) immiscibility with water and (3) high extraction ability for the target analytes. On the basis of these criteria, to achieve good extraction of carbamates from aqueous samples, alcohols 1-octanol, 1-nonanol, 1-decanol and 1-undecanol were chosen as potential extraction solvents. The final selection of solvent was decided on the basis of extraction efficiency. Comparison of EF obtained with each of the four extraction solvents showed that 1-octanol was the most effective; see Figure 2. It seems that carbamates have a better affinity for a slightly polar solvent, 1-octanol.
Figure 2.
Selection of extraction solvent (n = 3). Samples were spiked with 50 μg L-1 of each analyte. Extraction conditions: aqueous sample volume 10.0 mL; extraction solvent volume 15 μL; ultrasonication time: 5 min; salt addition: 1 g NaCl.
3.1.2. Effect of the volume of extraction solvent
The volume of the floating phase is increased with increasing volume of the extraction solvent; however, the analytes are diluted as a result of the increased volume of the floating phase. To estimate the influence of volume of extraction solvent on the procedure, the volume of extraction solvent (1-octanol) was varied in the range 8 to 15 μL. The results showed that the EF of the analytes decreased when the volume of the extraction solvent was added above 10 μL. This may be due to the dilution of extracts (Figure 3). Therefore, 10 μL of extraction solvent was chosen as the optimal volume for further studies.
Figure 3.
Effect of the extraction solvent volume (n = 3). Samples were spiked with 50 μg L-1 of each analyte. Extraction conditions: aqueous sample volume 10.0 mL; extraction solvent 1-octanol; ultrasonic time: 5 min; salt addition: 1 g NaCl.
3.1.3. Effect of ultrasonication time
The ultrasonication time might affect extraction efficiency because it affects both emulsification and the mass transfer process. To examine this effect, the EF was monitored with extraction times varying from 0 to 7 min (Figure 4). The maximum EF was achieved after ultrasonication for 3 min and no significant variation was observed with extraction times longer than 3 min. This is probably due to the fact that the ultrasonic water bath generates the emulsion quickly, rapidly making a very large contact surface area between the extraction solvent and the aqueous phase. Therefore 3 min was taken to be the optimum extraction time.
Figure 4.
Effect of ultrasonic extraction time (n = 3). Samples were spiked to 50 μg L-1 of each analyte. Extraction conditions: aqueous sample volume 10.0 mL; extraction solvent 1-octanol; extraction solvent volume 10 μL; salt addition: 1 g NaCl.
3.1.4. Effect of manual shaking
Manual shaking was essential to ensure that the extraction solvent and aqueous samples were adequately mixed before the ultrasound treatment when the ultrasonic extraction time was as brief as 3 min. A study done by Huang and co-workers [28] found that maximum peak area was achieved with 10 s of manual shaking and there was no significant increase for longer times. Here, with manual shaking, the EF was significantly higher than the EF value obtained from no manual shaking. The shaking assured that the aqueous sample was mixed well with the extraction solvent, thus generating the emulsion quickly (Figure 5).
Figure 5.
Effect of hand-shaking (n = 3). Samples were spiked to 50 μg L-1 of each analyte. Extraction conditions: aqueous sample volume 10.0 mL; extraction solvent 1-octanol; extraction solvent volume 10 μL; ultrasonication time: 3 min; salt addition: 1 g NaCl.
3.1.5. Effect of the amount of salt added
Ionic strength is an important determinant of extraction efficiency. During the extraction procedure, adding salt increases ionic strength, which leads to the “salting out” phenomenon, commonly discussed in liquid-liquid extraction. The presence of salt decreases the solubility of target analytes in aqueous phases, and improves their partition from aqueous to organic layer. This was observed on the increasing EFs when NaCl was added from 0 to 2.5 g (Figure 6). However, adding salt also eliminates the emulsion between aqueous and organic layers which results in the larger volume of floating phase left after extraction. When more salt was added, more floating phase was obtained. The larger volume of floating phase offsets the enhanced partition of the pesticides in organic layer. There was no significant change of the EF as the amount of salt added was increased from 2.5 to 3.0 g. On the basis of this result, 2.5 g of NaCl was added to the aqueous sample solution for further studies.
Figure 6.
Effect of salt addition (n = 3). Samples were spiked to 50 μg L-1 of each analyte. Extraction conditions: aqueous sample volume 10.0 mL; extraction solvent 1-octanol; extraction solvent volume 10 μL; ultrasonication time: 3 min.
3.2. Comparison of methods
The proposed method requires simple equipment: a 25 μL syringe, 10 μL of low toxicity extraction solvent, and an ultrasonic water bath, used to emulsify the extraction solvent and sample to form fine droplets for extraction. Compared to DLLME, solvent terminated dispersive liquid-liquid microextraction (ST-DLLME) and dispersive liquid-liquid microextraction combined with sweeping micellar electrokinetic chromatography (DLLME-sweeping-MEKC) method, this method avoids the use of a high toxicity extraction solvent and a large amount of dispersive solvent, which increases the solubility of the analytes in water during extraction. Unlike other extraction methods such as SPE, SPME, LPME, single drop microextraction (SDME) and quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based extraction, there is no need for this method to use a packed solid phase cartridge, a fragile fiber coated with a polymeric phase, a section of hollow fiber, a metal stand or clean-up sorbent. The apparatus needed for MS-USAEME is simpler than that required by the above-mentioned methods. According to the results in Table 1, the proposed method, which uses less organic solvent, maintains the advantage of short extraction times and makes the extraction more efficient.
g) QuEChERS: quick, easy, cheap, effective, rigged and safe procedure.
3.3. Analytical performance
Linearity (LR), regression coefficient (R2) and EF were investigated under optimized experimental conditions. The LR of the method was evaluated using water samples spiked with the selected compounds at various concentrations. The performance of the proposed method is summarized in Table 2. The linear calibration of the targeted carbamate pesticides was examined in the range 0.05 to 100 μg L-1. Linear plots yielded R2 ≥0.9972. The EF for all of the carbamate pesticides tested was in the range from 237 to 638. The results indicate that MS-USAEME combined with GC-MS was sensitive enough for the detection of these five carbamate pesticides.
In order to investigate the influence of the sample matrix in real samples, the determination of carbamate pesticides in water and vegetable matter was done using the method described here (Figure 7). All of the real samples were spiked with 5 μg L-1 methiocarb and 0.1 μg L-1 of the other four analytes to calculate the recovery of the targeted compounds. The reproducibility of the method was satisfactory; the RSD ranged from 6.8 to 16.9%. The relative recoveries were calculated by the ratios of the concentration of the carbamate pesticides in real samples and the concentrations of the analytes extracted in ultrapure water samples. Both samples were spiked with the same amount of pesticides. For all target compounds, the relative recoveries of field water and vegetables were within 77 to 114%. The definition of absolute recovery was determined by the ratio of extracted concentration in real samples and concentration spiked in the real sample. The absolute recoveries of target analytes were between 5.3 to 30.6%. The method detection limit (MDL) was calculated as three times the standard deviation of seven replicate runs of water and vegetable samples spiked with low concentrations of the analytes. MDL ranged from 0.013 to 0.022 μg L-1 for field water and 0.017 to 0.026 μg L-1 for the vegetable sample (Table 3).
Figure 7.
GC-MS chromatogram of A) field water sample and B) lettuce spiked with 5 carbamate pesticides at 0.5 µg L-1; Extraction conditions: samples volume 10.0 mL; extraction solvent 1-octanol; extraction solvent volume 10 μL; ultrasonication time: 3 min; salt addition : 2.5 g NaCl. 1. Propoxur. 2. Carbofuran. 3. Carbaryl. 4. Methiocarb. 5. Pirimicarb.
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t
\n\t\t\t
\n\t\t\t\tCompound\n\t\t\t
\n\t\t\t
\n\t\t\t\tField water\n\t\t\t
\n\t\t\t
\n\t\t\t\tVegetable sample\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
MDL(μg L-1)
\n\t\t\t
RSD%(n=6)
\n\t\t\t
Absoluterecovery(%)a\n\t\t\t
\n\t\t\t
Relative recovery(%)b\n\t\t\t
\n\t\t\t
MDL(μg L-1)
\n\t\t\t
RSD%(n=6)
\n\t\t\t
Absoluterecovery(%)a\n\t\t\t
\n\t\t\t
Relative recovery(%)b\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
Propoxur
\n\t\t\t
0.017
\n\t\t\t
8.2
\n\t\t\t
17.3
\n\t\t\t
82
\n\t\t\t
0.022
\n\t\t\t
7.9
\n\t\t\t
14.5
\n\t\t\t
98
\n\t\t
\n\t\t
\n\t\t\t
Carbofuran
\n\t\t\t
0.022
\n\t\t\t
10.9
\n\t\t\t
30.6
\n\t\t\t
114
\n\t\t\t
0.026
\n\t\t\t
7.2
\n\t\t\t
20.3
\n\t\t\t
110
\n\t\t
\n\t\t
\n\t\t\t
Carbaryl
\n\t\t\t
0.019
\n\t\t\t
6.8
\n\t\t\t
13.1
\n\t\t\t
77
\n\t\t\t
0.023
\n\t\t\t
11.3
\n\t\t\t
11.3
\n\t\t\t
96
\n\t\t
\n\t\t
\n\t\t\t
Methiocarb
\n\t\t\t
0.017
\n\t\t\t
16.9
\n\t\t\t
7.9
\n\t\t\t
110
\n\t\t\t
0.017
\n\t\t\t
8.0
\n\t\t\t
5.3
\n\t\t\t
105
\n\t\t
\n\t\t
\n\t\t\t
Pirimicarb
\n\t\t\t
0.013
\n\t\t\t
8.2
\n\t\t\t
10.3
\n\t\t\t
85
\n\t\t\t
0.023
\n\t\t\t
11.5
\n\t\t\t
8.4
\n\t\t\t
100
\n\t\t
\n\t
Table 3.
Analysis of real samples.
a Absolute recoveries were determined by the ratio of the extracted concentration to the spiked concentration in the real sample.
b Relative recoveries were determined by the ratio of the concentration found in the real sample to the concentration in deionized water samples. Both samples were spiked with the same amount of analytes. The extraction yields obtained from deionized water were considered as 100%.
Concentrations used for testing absolute recovery and relative recovery were: propoxur: 0.1 μg L-1; carbofuran: 0.1 μg L-1; carbaryl: 0.1 μg L-1; methiocarb: 5 μg L-1; pirimicarb: 0.1 μg L-1. MDL: spiked with 0.05 μg L-1 each compound.
4. Concluding remarks
This paper describes a simple, rapid extraction method using GC-MS to analyze carbamate pesticides in field aqueous and lettuce samples. Manual shaking for 10 s before ultrasonication is essential for effective extraction when the ultrasonication time is as brief as 3 min. The method performed well; repeatability, EF and recovery were satisfactory and the analysis can be done in a short time. Compared to other microextraction methods, the method described here is environmentally friendly and has the advantages of speed, simplicity, frugal use of organic solvent (10 μL/sample) and low cost.
Acknowledgments
This study was supported by the National Science Council of Taiwan (NSC 99-2113-M-007-004 -MY3).
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Reagents and materials",level:"2"},{id:"sec_3_2",title:"2.2. Sample preparation",level:"2"},{id:"sec_4_2",title:"2.3. Instrumentation",level:"2"},{id:"sec_5_2",title:"2.4. Analytical procedure",level:"2"},{id:"sec_7",title:"3. Results and discussion",level:"1"},{id:"sec_7_2",title:"3.1. Optimization of the experimental conditions",level:"2"},{id:"sec_7_3",title:"3.1.1. Selection of extraction solvent",level:"3"},{id:"sec_8_3",title:"3.1.2. Effect of the volume of extraction solvent",level:"3"},{id:"sec_9_3",title:"3.1.3. Effect of ultrasonication time",level:"3"},{id:"sec_10_3",title:"3.1.4. Effect of manual shaking",level:"3"},{id:"sec_11_3",title:"3.1.5. Effect of the amount of salt added",level:"3"},{id:"sec_13_2",title:"3.2. Comparison of methods",level:"2"},{id:"sec_14_2",title:"3.3. Analytical performance",level:"2"},{id:"sec_15_2",title:"3.4. Analysis of real samples",level:"2"},{id:"sec_17",title:"4. Concluding remarks",level:"1"},{id:"sec_18",title:"Acknowledgments",level:"1"},{id:"sec_18",title:"Acknowledgments",level:"2"}],chapterReferences:[{id:"B1",body:'YasuharaAShiraishiHNishikawaMYamamotoTUehiroTNakasugiOet alDetermination of organic components in leachates from hazardous waste disposal sites in Japan by gas chromatography-mass spectrometry. J Chromatogr A. 1997'},{id:"B2",body:'PuigDBarceloDDetermination of phenolic compounds in water and waste water. TRAC-TREND ANAL CHEM. 199615836275'},{id:"B3",body:'KellyM. TLarroqueMTrace determination of diethylphthalate in aqueous media by solid-phase microextraction-liquid chromatography. J Chromatogr A. 1999841217785'},{id:"B4",body:'MagdicSPawliszynJ. BAnalysis of organochlorine pesticides using solid-phase microextraction. J Chromatogr A. 1996723111122'},{id:"B5",body:'ArthurC. LPawliszynJSolid phase microextraction with thermal desorption using fused silica optical fibers. Anal Chem. 1990621921458'},{id:"B6",body:'HiroyukiKDerivatization reactions for the determination of amines by gas chromatography and their applications in environmental analysis. J Chromatogr A. 1996'},{id:"B7",body:'Acedo-valenzuelaM-IGaleano-díazTMora-díezNSilva-rodríguezADetermination of neutral and cationic herbicides in water by micellar electrokinetic capillary chromatography. Anal Chim Acta. 200451916571'},{id:"B8",body:'FeiTLiHDingMItoMLinJ-MDetermination of parabens in cosmetic products by solid-phase microextraction of poly(ethylene glycol) diacrylate thin film on fibers and ultra high-speed liquid chromatography with diode array detector. J Separ Sci. 201134131599606'},{id:"B9",body:'JeannotM. ACantwellF. FSolvent microextraction into a single drop. Anal Chem. 19966813223640'},{id:"B10",body:'ZhaoLLeeH. KApplication of static liquid-phase microextraction to the analysis of organochlorine pesticides in water. J Chromatogr A. 200191923818'},{id:"B11",body:'SarajiMFarajmandBApplication of single-drop microextraction combined with in-microvial derivatization for determination of acidic herbicides in water samples by gas chromatography-mass spectrometry. J Chromatogr A. 2008'},{id:"B12",body:'AhmadiFAssadiYHosseini SMRM, Rezaee M. Determination of organophosphorus pesticides in water samples by single drop microextraction and gas chromatography-flame photometric detector. J Chromatogr A. 2006'},{id:"B13",body:'JeannotM. ACantwellF. FSolvent microextraction as a speciation tool: determination of free progesterone in a protein solution. Anal Chem. 19976915293540'},{id:"B14",body:'JiangXLeeH. KSolvent Bar Microextraction. Anal Chem. 2004761855916'},{id:"B15",body:'MelwankiM. BHuangS-DFuhM-RThree-phase solvent bar microextraction and determination of trace amounts of clenbuterol in human urine by liquid chromatography and electrospray tandem mass spectrometry. Talanta. 20077223737'},{id:"B16",body:'ShenGLeeH. KHollow fiber-protected liquid-phase microextraction of triazine herbicides. Anal Chem. 200174364854'},{id:"B17",body:'ChenP-SHuangS-PFuhM-RHuangS-DDetermination of organochlorine pesticides in water using dynamic hook-type liquid-phase microextraction. Anal Chim Acta. 2009647217781'},{id:"B18",body:'PsillakisEKalogerakisNHollow-fibre liquid-phase microextraction of phthalate esters from water. J Chromatogr A. 2003'},{id:"B19",body:'HuangS-PChenP-SHuangS-DDynamic headspace time-extended helix liquid-phase microextraction. J Chromatogr A. 2009121620434753'},{id:"B20",body:'RezaeeMAssadiYMilani Hosseini M-R, Aghaee E, Ahmadi F, Berijani S. Determination of organic compounds in water using dispersive liquid-liquid microextraction. J Chromatogr A. 2006'},{id:"B21",body:'FarajzadehM. ABahramMJonssonJ. ADispersive liquid-liquid microextraction followed by high-performance liquid chromatography-diode array detection as an efficient and sensitive technique for determination of antioxidants. Anal Chim Acta. 200759116979'},{id:"B22",body:'NagarajuDHuangS-DDetermination of triazine herbicides in aqueous samples by dispersive liquid-liquid microextraction with gas chromatography-ion trap mass spectrometry. J Chromatogr A. 2007'},{id:"B23",body:'RegueiroJLlompartMGarcia-jaresCGarcia-monteagudoJ. CCelaRUltrasound-assisted emulsification-Microextraction of emergent contaminants and pesticides in environmental waters. J Chromatogr A. 2008'},{id:"B24",body:'WangW-XYangT-JLiZ-GJongT-TLeeM-RA novel method of ultrasound-assisted dispersive liquid-liquid microextraction coupled to liquid chromatography-mass spectrometry for the determination of trace organoarsenic compounds in edible oil. Anal Chim Acta. 201169022217'},{id:"B25",body:'OzcanSTorAAydinM. EDetermination of selected polychlorinated biphenyls in water samples by ultrasound-assisted emulsification-microextraction and gas chromatography-mass-selective detection. Anal Chim Acta. 200964721828'},{id:"B26",body:'LinS-LFuhM-ROrthogonal array optimization of ultrasound-assisted emulsification microextraction for the determination of chlorinated phenoxyacetic acids in river water. J Chromatogr A. 2010121721346772'},{id:"B27",body:'FontanaA. RCamargoA. BAltamiranoJ. CCoacervative microextraction ultrasound-assisted back-extraction technique for determination of organophosphates pesticides in honey samples by gas chromatography-mass spectrometry. J Chromatogr A. 2010121741633441'},{id:"B28",body:'WeiS-YLeongM-ILiYHuangS-DDevelopment of liquid phase microextraction based on manual shaking and ultrasound-assisted emulsification method for analysis of organochlorine pesticides in aqueous samples. J Chromatogr A. 201112185191428'},{id:"B29",body:'WuY. SLeeH. KLi SFY. Separation and determination of pesticides by capillary electrophoresis. I. Rapid separation of fifteen N-methylcarbamate insecticides via micellar electrokinetic chromatography. J Microcolumn Sep. 199810323947'},{id:"B30",body:'ZhangJLeeH. KApplication of liquid-phase microextraction and on-column derivatization combined with gas chromatography-mass spectrometry to the determination of carbamate pesticides. J Chromatogr A. 200611171317'},{id:"B31",body:'MolinaMPérez-benditoDSilvaMMulti-residue analysis of N-methylcarbamate pesticides and their hydrolytic metabolites in environmental waters by use of solid-phase extraction and micellar electrokinetic chromatography. Electrophoresis. 19992017343949'},{id:"B32",body:'HyltonKMitraSBarrier film protected, and mixed solvent optimized micro-scale membrane extraction of methyl carbamate pesticides. J Chromatogr A. 2007'},{id:"B33",body:'Carabias-martínezRGarcía-hermidaCRodríguez-gonzaloERuano-miguelLBehaviour of carbamate pesticides in gas chromatography and their determination with solid-phase extraction and solid-phase microextraction as preconcentration steps. J Separ Sci. 2005281621308'},{id:"B34",body:'SagratiniGManesJDarioGDamianiPPicoYAnalysis of carbamate and phenylurea pesticide residues in fruit juices by solid-phase microextraction and liquid chromatography-mass spectrometry. J Chromatogr A. 20071147213543'},{id:"B35",body:'WuQZhouXLiYZangXWangCWangZApplication of dispersive liquid-liquid microextraction combined with high-performance liquid chromatography to the determination of carbamate pesticides in water samples. Anal Bioanal Chem. 20093936175561'},{id:"B36",body:'ChenHChenRLiSLow-density extraction solvent-based solvent terminated dispersive liquid-liquid microextraction combined with gas chromatography-tandem mass spectrometry for the determination of carbamate pesticides in water samples. J Chromatogr A. 20091217812448'},{id:"B37",body:'WuQChangQWuCRaoHZengXWangCet alUltrasound-assisted surfactant-enhanced emulsification microextraction for the determination of carbamate pesticides in water samples by high performance liquid chromatography. J Chromatogr A. 201012171117738'},{id:"B38",body:'ZhangSLiCSongSFengTWangCWangZApplication of dispersive liquid-liquid microextraction combined with sweeping micellar electrokinetic chromatography for trace analysis of six carbamate pesticides in apples. Anal Methods. 2010215462'},{id:"B39",body:'ChenHChenRFengRLiSSimultaneous Analysis of Carbamate and Organophosphorus Pesticides in Water by Single-Drop Microextraction Coupled with GC-MS. 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Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan
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Response to Pendimethalin Formulation, Timing, and Method of Application",slug:"cotton-gossypium-hirsutum-l-response-to-pendimethalin-formulation-timing-and-method-of-application",signatures:"Timothy Grey and Theodore Webster",authors:[{id:"13772",title:"Dr.",name:"Timothy",middleName:null,surname:"Grey",fullName:"Timothy Grey",slug:"timothy-grey"}]},{id:"44928",title:"Herbicide — Soil Interactions, Applied to Maize Crop Under Brazilian Conditions",slug:"herbicide-soil-interactions-applied-to-maize-crop-under-brazilian-conditions",signatures:"Flavio Martins Garcia Blanco, Sydnei Dionisio Batista de Almeida\nand Marcus Barifouse Matallo",authors:[{id:"90516",title:"Dr.",name:"Flavio",middleName:"Martins Garcia",surname:"Blanco",fullName:"Flavio Blanco",slug:"flavio-blanco"},{id:"167471",title:"BSc.",name:"Sydnei",middleName:null,surname:"Almeida",fullName:"Sydnei Almeida",slug:"sydnei-almeida"},{id:"167472",title:"Dr.",name:"Marcus",middleName:null,surname:"Matallo",fullName:"Marcus Matallo",slug:"marcus-matallo"}]},{id:"44271",title:"Integration of Allelopathy to Control Weeds in Rice",slug:"integration-of-allelopathy-to-control-weeds-in-rice",signatures:"T.D. Khanh, L.H. Linh, T.H. Linh, N.T. Quan, D.M. Cuong, V.T.T. Hien,\nL.H. Ham and T.D. Xuan",authors:[{id:"160517",title:"Dr.",name:"Tran",middleName:"Dang",surname:"Khanh",fullName:"Tran Khanh",slug:"tran-khanh"},{id:"162489",title:"Dr.",name:"Le Hung",middleName:null,surname:"Linh",fullName:"Le Hung Linh",slug:"le-hung-linh"},{id:"162490",title:"Prof.",name:"Le Huy",middleName:null,surname:"Ham",fullName:"Le Huy Ham",slug:"le-huy-ham"},{id:"162491",title:"Prof.",name:"Tran Dang",middleName:null,surname:"Xuan",fullName:"Tran Dang Xuan",slug:"tran-dang-xuan"},{id:"167870",title:"Dr.",name:"Ta Hong",middleName:null,surname:"Linh",fullName:"Ta Hong Linh",slug:"ta-hong-linh"},{id:"167871",title:"MSc.",name:"Nguyen Thanh",middleName:null,surname:"Quan",fullName:"Nguyen Thanh Quan",slug:"nguyen-thanh-quan"},{id:"167872",title:"BSc.",name:"Do Manh",middleName:null,surname:"Cuong",fullName:"Do Manh Cuong",slug:"do-manh-cuong"},{id:"167873",title:"Dr.",name:"Vu Thi Thu",middleName:null,surname:"Hien",fullName:"Vu Thi Thu Hien",slug:"vu-thi-thu-hien"}]},{id:"44941",title:"Weed and Disease Control and Peanut Response Following Post—Emergence Herbicide and Fungicide Combinations",slug:"weed-and-disease-control-and-peanut-response-following-post-emergence-herbicide-and-fungicide-combin",signatures:"W. James Grichar, Peter A. Dotray and Jason E. Woodward",authors:[{id:"13502",title:"Prof.",name:"W. James",middleName:null,surname:"Grichar",fullName:"W. James Grichar",slug:"w.-james-grichar"},{id:"14189",title:"Dr.",name:"Jason",middleName:null,surname:"Woodward",fullName:"Jason Woodward",slug:"jason-woodward"},{id:"14656",title:"Dr.",name:"Peter A.",middleName:null,surname:"Dotray",fullName:"Peter A. Dotray",slug:"peter-a.-dotray"}]},{id:"44995",title:"Weed Management in Cereals in Semi-Arid Environments: A Review",slug:"weed-management-in-cereals-in-semi-arid-environments-a-review",signatures:"Inés Santín-Montanyá, Encarnación Zambrana-Quesada and José\nLuis Tenorio-Pasamón",authors:[{id:"13725",title:"Dr.",name:"Ines",middleName:null,surname:"Santin-Montanya",fullName:"Ines Santin-Montanya",slug:"ines-santin-montanya"}]},{id:"44988",title:"The Use of Glyphosate in Sugarcane: A Brazilian Experience",slug:"the-use-of-glyphosate-in-sugarcane-a-brazilian-experience",signatures:"Carlos Alberto Mathias Azania, Luciana Rossini Pinto, Rodrigo\nCabral Adriano, Dilermando Perecin and Andréa Padua Azania",authors:[{id:"14110",title:"Dr.",name:"Andrea",middleName:null,surname:"Azania",fullName:"Andrea Azania",slug:"andrea-azania"},{id:"14112",title:"Dr.",name:"Carlos",middleName:null,surname:"Azania",fullName:"Carlos Azania",slug:"carlos-azania"},{id:"162653",title:"Dr.",name:"Luciana",middleName:null,surname:"Rossini",fullName:"Luciana Rossini",slug:"luciana-rossini"},{id:"166193",title:"MSc.",name:"Rodrigo",middleName:null,surname:"Adriano",fullName:"Rodrigo Adriano",slug:"rodrigo-adriano"},{id:"166506",title:"Prof.",name:"Dilermando",middleName:null,surname:"Perecin",fullName:"Dilermando Perecin",slug:"dilermando-perecin"}]},{id:"43790",title:"Herbicides Used in Tobacco",slug:"herbicides-used-in-tobacco",signatures:"William A. Bailey",authors:[{id:"163443",title:"Dr.",name:"William",middleName:"Anthony",surname:"Bailey",fullName:"William Bailey",slug:"william-bailey"}]},{id:"44993",title:"Herbicides for Natural Area Weed Management",slug:"herbicides-for-natural-area-weed-management",signatures:"Gregory E. MacDonald, Lyn A. Gettys, Jason A. Ferrell and Brent A.\nSellers",authors:[{id:"161877",title:"Dr.",name:"Lyn",middleName:null,surname:"Gettys",fullName:"Lyn Gettys",slug:"lyn-gettys"},{id:"162844",title:"Dr.",name:"Greg",middleName:null,surname:"MacDonald",fullName:"Greg MacDonald",slug:"greg-macdonald"},{id:"168391",title:"Dr.",name:"Jason",middleName:null,surname:"Ferrell",fullName:"Jason Ferrell",slug:"jason-ferrell"},{id:"168392",title:"Dr.",name:"Brent",middleName:null,surname:"Sellers",fullName:"Brent Sellers",slug:"brent-sellers"}]},{id:"44996",title:"Integrated Weed Management Practices for Adoption in the Tropics",slug:"integrated-weed-management-practices-for-adoption-in-the-tropics",signatures:"Wendy-Ann P. Isaac, Puran Bridgemohan and Wayne G. Ganpat",authors:[{id:"89206",title:"Dr.",name:"Wendy-Ann",middleName:"Patrice",surname:"Isaac",fullName:"Wendy-Ann Isaac",slug:"wendy-ann-isaac"},{id:"127534",title:"Dr.",name:"Wayne",middleName:null,surname:"Ganpat",fullName:"Wayne Ganpat",slug:"wayne-ganpat"},{id:"160555",title:"Dr.",name:"Puran",middleName:null,surname:"Bridgemohan",fullName:"Puran Bridgemohan",slug:"puran-bridgemohan"}]},{id:"44961",title:"Integrated Plant Invasion and Bush Encroachment Management on Southern African Rangelands",slug:"integrated-plant-invasion-and-bush-encroachment-management-on-southern-african-rangelands",signatures:"M. S. Lesoli, M. Gxasheka, T. B. Solomon and B. Moyo",authors:[{id:"14095",title:"Dr.",name:"\\'Mota",middleName:"Samuel",surname:"Lesoli",fullName:"\\'Mota Lesoli",slug:"'mota-lesoli"},{id:"168342",title:"Dr.",name:"Beyene",middleName:null,surname:"Solomon",fullName:"Beyene Solomon",slug:"beyene-solomon"},{id:"168343",title:"Mr.",name:"Masibonge",middleName:null,surname:"Gxasheka",fullName:"Masibonge Gxasheka",slug:"masibonge-gxasheka"},{id:"168380",title:"Dr.",name:"Bethwell",middleName:null,surname:"Moyo",fullName:"Bethwell Moyo",slug:"bethwell-moyo"}]},{id:"44963",title:"New Natural Herbicide Candidate for Sicyon angulatus Control",slug:"new-natural-herbicide-candidate-for-sicyon-angulatus-control",signatures:"Jung-Sup Choi and In-Taek Hwang",authors:[{id:"14070",title:"Dr.",name:"In-Taek",middleName:null,surname:"Hwang",fullName:"In-Taek Hwang",slug:"in-taek-hwang"},{id:"162605",title:"Dr.",name:"Jung-Sup",middleName:null,surname:"Choi",fullName:"Jung-Sup Choi",slug:"jung-sup-choi"}]},{id:"43449",title:"Herbicides in Aquatic Systems",slug:"herbicides-in-aquatic-systems",signatures:"Lyn A. Gettys, William T. Haller and Gregory E. MacDonald",authors:[{id:"161877",title:"Dr.",name:"Lyn",middleName:null,surname:"Gettys",fullName:"Lyn Gettys",slug:"lyn-gettys"},{id:"162844",title:"Dr.",name:"Greg",middleName:null,surname:"MacDonald",fullName:"Greg MacDonald",slug:"greg-macdonald"},{id:"167830",title:"Dr.",name:"William",middleName:null,surname:"Haller",fullName:"William Haller",slug:"william-haller"}]},{id:"44978",title:"Herbicide Impact on Seagrass Communities",slug:"herbicide-impact-on-seagrass-communities",signatures:"A. Damien Devault and Hélène Pascaline",authors:[{id:"162714",title:"Dr.",name:"Damien",middleName:"A.",surname:"Devault",fullName:"Damien Devault",slug:"damien-devault"}]},{id:"44983",title:"Transgenic Herbicide-Resistant Turfgrasses",slug:"transgenic-herbicide-resistant-turfgrasses",signatures:"In-Ja Song, Tae-Woong Bae, Markkandan Ganesan, Jeong-Il Kim,\nHyo-Yeon Lee and Pill-Soon Song",authors:[{id:"162059",title:"Dr.",name:"In-Ja",middleName:null,surname:"Song",fullName:"In-Ja Song",slug:"in-ja-song"},{id:"162060",title:"Dr.",name:"Hyo-Yeon",middleName:null,surname:"Lee",fullName:"Hyo-Yeon Lee",slug:"hyo-yeon-lee"},{id:"163639",title:"Prof.",name:"Pill-Soon",middleName:null,surname:"Song",fullName:"Pill-Soon Song",slug:"pill-soon-song"},{id:"163871",title:"Dr.",name:"Markkandan",middleName:null,surname:"Ganesan",fullName:"Markkandan Ganesan",slug:"markkandan-ganesan"},{id:"163872",title:"Prof.",name:"Jeong-Il",middleName:null,surname:"Kim",fullName:"Jeong-Il Kim",slug:"jeong-il-kim"},{id:"167842",title:"Dr.",name:"Tae-Woong",middleName:null,surname:"Bae",fullName:"Tae-Woong Bae",slug:"tae-woong-bae"}]},{id:"44984",title:"Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health",slug:"toxicity-of-herbicides-impact-on-aquatic-and-soil-biota-and-human-health",signatures:"Maria Aparecida Marin-Morales, Bruna de Campos Ventura-\nCamargo and Márcia Miyuki Hoshina",authors:[{id:"163013",title:"Dr",name:"Maria Aparecida",middleName:null,surname:"Marin-Morales",fullName:"Maria Aparecida Marin-Morales",slug:"maria-aparecida-marin-morales"}]},{id:"44981",title:"Herbicide Resistant Weeds: The Technology and Weed Management",slug:"herbicide-resistant-weeds-the-technology-and-weed-management",signatures:"Jamal R. Qasem",authors:[{id:"13565",title:"Prof.",name:"Jamal R.",middleName:null,surname:"Qasem",fullName:"Jamal R. Qasem",slug:"jamal-r.-qasem"}]},{id:"44957",title:"Pesticide Tank Mixes: An Environmental Point of View",slug:"pesticide-tank-mixes-an-environmental-point-of-view",signatures:"Valdemar Luiz Tornisielo, Rafael Grossi Botelho, Paulo Alexandre de\nToledo Alves, Eloana Janice Bonfleur and Sergio Henrique Monteiro",authors:[{id:"91920",title:"Ph.D.",name:"Rafael",middleName:"Grossi",surname:"Grossi Botelho",fullName:"Rafael Grossi Botelho",slug:"rafael-grossi-botelho"},{id:"162791",title:"Prof.",name:"Valdemar",middleName:null,surname:"Tornisielo",fullName:"Valdemar Tornisielo",slug:"valdemar-tornisielo"},{id:"162792",title:"MSc.",name:"Paulo",middleName:null,surname:"Alves",fullName:"Paulo Alves",slug:"paulo-alves"},{id:"167275",title:"M.Sc.",name:"Sérgio",middleName:"Henrique",surname:"Monteiro",fullName:"Sérgio Monteiro",slug:"sergio-monteiro"},{id:"167277",title:"MSc.",name:"Eloana",middleName:null,surname:"Bonfleur",fullName:"Eloana Bonfleur",slug:"eloana-bonfleur"}]},{id:"44986",title:"Characterization, Modes of Action and Effects of Trifluralin: A Review",slug:"characterization-modes-of-action-and-effects-of-trifluralin-a-review",signatures:"Thaís C. C. Fernandes, Marcos A. Pizano and Maria A. Marin-Morales",authors:[{id:"163013",title:"Dr",name:"Maria Aparecida",middleName:null,surname:"Marin-Morales",fullName:"Maria Aparecida Marin-Morales",slug:"maria-aparecida-marin-morales"}]},{id:"44466",title:"Allelochemicals as Bioherbicides — Present and Perspectives",slug:"allelochemicals-as-bioherbicides-present-and-perspectives",signatures:"Dorota Soltys, Urszula Krasuska, Renata Bogatek and Agnieszka\nGniazdowska",authors:[{id:"162641",title:"Dr.",name:"Dorota",middleName:null,surname:"Soltys",fullName:"Dorota Soltys",slug:"dorota-soltys"},{id:"162661",title:"Dr.",name:"Agnieszka",middleName:null,surname:"Gniazdowska",fullName:"Agnieszka Gniazdowska",slug:"agnieszka-gniazdowska"},{id:"162664",title:"Prof.",name:"Renata",middleName:null,surname:"Bogatek",fullName:"Renata Bogatek",slug:"renata-bogatek"},{id:"167788",title:"Dr.",name:"Urszula",middleName:null,surname:"Krasuska",fullName:"Urszula Krasuska",slug:"urszula-krasuska"}]},{id:"44759",title:"Managing Commelina Species: Prospects and Limitations",slug:"managing-commelina-species-prospects-and-limitations",signatures:"Wendy-Ann Isaac, Zongjun Gao and Mei Li",authors:[{id:"89206",title:"Dr.",name:"Wendy-Ann",middleName:"Patrice",surname:"Isaac",fullName:"Wendy-Ann Isaac",slug:"wendy-ann-isaac"},{id:"164493",title:"M.Sc.",name:"Zongjun",middleName:null,surname:"Gao",fullName:"Zongjun Gao",slug:"zongjun-gao"},{id:"166083",title:"Dr.",name:"Mei",middleName:null,surname:"Li",fullName:"Mei Li",slug:"mei-li"}]},{id:"43766",title:"Integrating Herbicides in a High-Residue Cover Crop Setting",slug:"integrating-herbicides-in-a-high-residue-cover-crop-setting",signatures:"Andrew J. Price and Jessica A. Kelton",authors:[{id:"13747",title:"Dr.",name:"Andrew",middleName:null,surname:"Price",fullName:"Andrew Price",slug:"andrew-price"},{id:"13748",title:"Prof.",name:"Jessica",middleName:null,surname:"Kelton",fullName:"Jessica Kelton",slug:"jessica-kelton"}]},{id:"44977",title:"Herbicide Safeners: Effective Tools to Improve Herbicide Selectivity",slug:"herbicide-safeners-effective-tools-to-improve-herbicide-selectivity",signatures:"Istvan Jablonkai",authors:[{id:"86229",title:"Dr.",name:"Istvan",middleName:null,surname:"Jablonkai",fullName:"Istvan Jablonkai",slug:"istvan-jablonkai"}]},{id:"44049",title:"Herbicides — A Double Edged Sword",slug:"herbicides-a-double-edged-sword",signatures:"Mona H. El-Hadary and Gyuhwa Chung",authors:[{id:"91315",title:"Prof.",name:"Gyuhwa",middleName:null,surname:"Chung",fullName:"Gyuhwa Chung",slug:"gyuhwa-chung"},{id:"165870",title:"Dr.",name:"Mona",middleName:null,surname:"El-Hadary",fullName:"Mona El-Hadary",slug:"mona-el-hadary"}]}]}]},onlineFirst:{chapter:{type:"chapter",id:"66606",title:"Introductory Chapter: Transcriptome Analysis",doi:"10.5772/intechopen.85980",slug:"introductory-chapter-transcriptome-analysis",body:'\n
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The central dogma of molecular biology describes the flow of genetic information from genes to functions of the cells and organisms. This comprises a two-step process: first, DNA, the permanent, heritable, genetic information repository, is transcribed by the RNA polymerase enzymes into RNA, a short-lasting information carrier; second, a subset of RNA, the messenger RNAs, mRNAs, are translated into protein. The transcriptome, then, is the complete set of all RNA molecules in a cell, a population of cells or in an organism.
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Importantly, not all RNAs are translated into proteins, some serve a structural function, for example, rRNAs in the assembly of ribosomes, others are transporters, e.g., tRNAs, yet others serve regulatory functions, for example, the siRNAs, short interfering RNA, or lncRNAs, long non-coding RNAs; these are not translated into proteins [1]. However, these non-coding RNAs can and often do play roles in human diseases such as cancer, cardiovascular, and neurological disorders. While transcriptomics is most commonly applied to the mRNAs, the coding transcripts, transcriptomics also provides important data regarding content of the cell noncoding RNAs, including rRNA, tRNA, lncRNA, siRNA, and others. Specific approaches address the analysis of splice variant of the same gene in different tissues.
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1. Transcriptome analysis
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Transcriptome Analysis is the study of the transcriptome, of the complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific cell, using high-throughput methods. Transcription profiling, which follows changes in behavior of a cell in toto, not of a single gene or just a few genes, is used throughout diverse areas of biomedical research, including disease diagnosis, biomarker discovery, risk assessment of new drugs or environmental chemicals etc. Transcription profiling can be applied to loss- and gain-of-function mutants to identify the changes associated with the mutant phenotype. The transcriptomic techniques have been particularly useful in identifying the functions of genes. Transcriptomics also allows identification of pathways that respond to or ameliorate environmental stresses. RNA-Seq can also identify disease-associated gene fusions, single nucleotide polymorphisms and even allele-specific expression.
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2. Uses of transcriptome analysis
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Transcriptome Analysis is most commonly used to compare specific pairs of samples. The differences may be due to different external environmental conditions, e.g., hormonal effects or toxins. More commonly, healthy and disease states are compared. For example, in cancer, transcriptomics analyses address classification, the mechanisms of pathogenesis and even outcome prediction. Transcriptome studies can classify cancer beyond anatomical location and histopathology. Outcome predictions can establish gene-based benchmarks to predict tumor prognosis and therapy response. These approaches are already in use for personalized medicine, individualized cancer patient therapies.
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Organisms and tissues at various stages of development can be molecularly characterized. The transcriptomes of stem cells help to understand the processes of cellular differentiation or embryonic development. Because of its very broad approach transcriptome analysis is a great source for identifying targets for treatment.
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2.1 Methodologies
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The early approach to study whole transcriptomes used microarrays, a set of defined sequences arranged on a solid substrate [2]. Microarrays almost exclusively represented mRNAs, that is, genes that are translated into proteins.
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Nowadays the microarray approach is supplanted by high-throughput RNA sequencing, RNA-Seq, which detects all transcripts in a sample, including the regulatory siRNA and lncRNA transcripts [3]. In this methodology, the bulk RNA is extracted from the sample and copied into stable double-stranded copy DNA, ds-cDNA, which is then sequenced using various sequencing methods [4]. The sequences obtained are aligned to reference genome sequences, available in data banks, to identify which genes are transcribed. Quantitatively, the results provide the expression levels for the transcribed genes. Compared to microarrays, RNA-Seq can measure both the low-abundance and high-abundance RNAs over a five orders of magnitude range and, importantly, RNA-Seq requires much less starting material (nanograms vs. micrograms and even as little as 50 pg) [5]. This made possible analyses of transcriptomes in a single cell, a great advance over bulk tissue RNA analyses [6]. RNA-seq can be used to identify alternative splicing, novel transcripts, and fusion genes (Table 1).
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Table 1.
Comparison of RNA-seq methodologies.
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In principle, the assembly of RNA-Seq reads is not dependent on reference genomes and can be used for gene expression studies of poorly characterized species with limited genomic resources. It can also be used to identify novel protein coding regions in sequenced genomes. RNA-seq can be performed using many next-generation sequencing platforms, however, each platform has its own requirements of sample preparation and the instrument design.
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2.2 Data analysis, repositories and presentation
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Improved sequencing technologies necessitated improved data analysis methods to deal with the increased volume of data produced by each transcriptome experiment. Importantly, the results are deposited into transcriptome databases, essential tools for transcriptome analysis. For example Gene Expression Omnibus, www.ncbi.nml.nih.gov, contains millions of transcription profiling experiments. Such data have potential applications beyond the original aims of an experiment. Typical outputs include quantitative tables of the transcript levels. This requires specific analysis algorithms, often specific to the methodology used. There are software packages to bridge data from disparate methodologies, to identify groups of similar expressed genes, or differentially expressed functionally significant regulatory or metabolic pathways.
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The results of transcriptomic analyses are graphically often presented as heat maps, a system of color-coding that represents different levels of expression of given genes in different samples (Figure 1A). Such presentations also frequently display a clustering of samples, this helps to identify samples with similar gene expression. Another common graphical presentation uses Venn diagrams, which count the transcripts which are equivalently regulated in multiple samples (Figure 1B).
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Figure 1.
Graphic representations of transcriptome analysis data. (A) Heat map with clustering tree. (B) Venn diagrams of regulated genes.
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Transcriptome analyses have become indispensable in basic research, translational, and clinical studies. In general, transcriptome analysis is a very powerful hypothesis-generating tool, more than a theory proving one.
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3. Specific example: transcriptome analysis applied to human skin
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Easily accessible, skin was among the first targets analyzed using ‘omics’ and dermatology embraced the approaches very early [7]. A classic example of coordinated transcriptional regulation was observed in cultured fibroblasts after serum stimulation [2]. Serum addition causes not only rapid recommencement of the cell cycle but, characteristically a wound-healing response, a physiological role of fibroblasts in wound healing [8]. Transcriptional responses of epidermal keratinocytes to UV light, hormones, vitamins, infections, inflammatory and immunomodulating cytokines, toxins and allergens have been characterized, as were the changes associated with epidermal differentiation [9, 10].
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The expression signatures that define the various cell types in human skin, were used to define 20 specific gene signatures, including those for keratinocytes, melanocytes, endothelia, adipocytes, immune cells, hair follicles, sebaceous, sweat, and apocrine glands. This resource provided a resource named SkinSig, which was then used to analyze 18 skin conditions, providing in-context interpretation of, for example, influx in immune cells in inflammation or differentiation changes in disorders of cornification [11].
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In the future we can anticipate a greatly expanded usage of transcriptome analysis. Translated to the bedside, it can provide better understanding and more specific diagnoses of diseases. This, of course, requires additional advances in the technology, both in the lab-bench components reducing the costs and guaranteeing reproducibility and accuracy, as well as in the computer-based components, algorithms that enable physicians to establish diagnosis quickly and reliably. In a generation, this approach will become routine.
\n\n
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Openness - We communicate honestly and transparently. We are open to constructive criticism and committed to learning from it.
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If this sounds like a place that you would like to work, whether you are at the beginning of your career or are an experienced professional, we invite you to drop us a line and tell us why you could be the right person for IntechOpen.
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