In this paper, an attempt has been made to check the level of surfactants particularly in drinking water, which can lead to toxicity in human body system. In this study, a total of 10 locations were selected to enumerate the concentration of surfactants and other physicochemical parameters with metals in the flowing water of river during pre-monsoon 2019. Analyzed result showed that the concentration of surfactants was significantly high and other parameters were also high. It was also found that river at the vicinity of town areas or midstream in the Lucknow city contained high amount of an anionic surfactants due to the nonpoint sources generated by human activities, low concentration was found in upstream, and average concentration was found in downstream, showing natural degradation of surfactants. The values of other parameters were higher than the prescribed limit, which is the serious problem for human being.