\r\n\tIt is a relatively simple process and a standard tool in any industry. Because of the versatility of the titration techniques, nearly all aspects of society depend on various forms of titration to analyze key chemical compounds.
\r\n\tThe aims of this book is to provide the reader with an up-to-date coverage of experimental and theoretical aspects related to titration techniques used in environmental, pharmaceutical, biomedical and food sciences.
Oxygen radicals are continuously formed in all living organisms, with deleterious effects that lead to cell injury and death. Production of oxidative species occurs under physiological conditions at a controlled rate, but it is dramatically increased in conditions of oxidative stress. Reactive oxygen species (ROS) is a term which encompasses all highly reactive, oxygen-containing molecules, including free radicals [1, 2]. Free radicals are an atom or molecule that bears an unpaired electron and is extremely reactive, capable of engaging in rapid change reaction that destabilize other molecules and generate many more free radicals [3, 4]. Vitamin E compounds (tocopherols and tocotrienols) are well recognized for their effective inhibition of lipid oxidation in food and biological systems [5, 2]. Carotenoids can act as primary antioxidants by trapping free radicals or as secondary antioxidants by quenching singlet oxygen. In foods, carotenoids usually act as a secondary antioxidant. Beta-carotene is found in many foods that are orange in color [6].
Stress plays a significant role in the development of atherosclerotic heart disease (AHD) [7]. A stressful condition leads to the excessive production of free radicals which results in oxidative stress an imbalance in the oxidant per antioxidant system [8]. Under normal conditions, there is a natural defense system provided by several enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) which performs a vital role for detoxification of free radicals. The use of antioxidant rich food or antioxidant food supplements became immensely popular since many diseases have been associated with oxidative stress [9]. Antioxidant enzyme such as superoxide dismutase (SOD) is an important radical superoxide scavenger and it plays an important role in cell protection [10, 2]. Therefore, this CAT or SOD enzyme are very good biochemical markers of stress and their increased activity may attest to a potential for remediation [11]. Inherent antioxidant defense systems consisting of enzymes, such as catalase (CAT), and superoxide dismutase (SOD), an antioxidant nutrients may participate in coping with oxidative stress. As antioxidant enzymes have an important role in the protection against free radical damage, a decrease in the activities or expression of these enzymes may predispose tissues to free radical damage [12].
Red palm oil (RPO) is extracted from the oil palm (
Corn oil presents a relatively high concentration of polyunsaturated fatty acids (PUFA).Due to the high levels of unsaturation these lipids are highly susceptible to free radical oxidative reactions, giving rise to the formation of lipid peroxides. Many investigations suggest that a large number of polyunsaturated fatty acids produces more lipid peroxides and may have mutagenic activity [18, 19].
Coconut oil is a colorless to pale, brownish yellow oil [20]. It is the major sources of saturated fat apart from palm kernel. They are the only natural sources of lauric oil available to the world market. Coconut oil is the principal cholesterol-raising fat because it contains large amounts of lauric (C: 12: O) and myristic (C: 14: 0) acids [21]. Therefore the objective of this study is to investigate the effect of different concentration of red palm olein and different vegetable oils on antioxidant enzymes in normal and stressed rats.
Due to the importance of the role of antioxidants in protection against the oxidative stress which lead to many dangerous diseases such as heart diseases and cancer thus this study was done to investigate the effect of natural antioxidants particularly vitamin E and beta carotene in red palm olein on antioxidant enzymes and compared the results with four different vegetable oils in normal and stress conditions of rats.
The evaluated red palm olein (RPO) samples consisted of carotenes (576 ppm), vitamin E (>800 ppm) and free fatty acids (0.045%) provided by Carotino SDN BHD company and palm olein (PO) (Seri Murni), corn oil (CO) and coconut oil (COC) were obtained commercially. For the first group the test diet was prepared by mixing RPO with normal commercial rat pellet to contain 5%, 10% and 15% of the red palm olein (RPO). The 5% diet was prepared by adding 5g RPO to 95g rat pellet, and mixed manually and the diets were then left to absorb the RPO at room temperature overnight and stored at 20o C before the feeding trial was conducted. Similar process was conducted with 10%, and 15% RPO. For second group the test diet was prepared by mixing vegetable oils with normal commercial rat pellet to contain 15% of the vegetable oils. The 15% diet was prepared by adding 15g RPO, PO, CO or COC to 85g rat pellet, and mixed manually and the diets were then left to absorb the vegetable oils at room temperature overnight and stored at 20o C before the feeding trial was conducted.
Normal (N) group: Rats were maintained under standard laboratory conditions and fed with respective diet till the completion of the experiment.Normal rats
Stressed rats
One hundred and eighty Sprague Dawley male rats each weighing between 170-250g and approximately 80 days old were obtained from the animal house of the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. They were divided into three groups.
The first group contains 78 rats were divided into 13 groups of 6 rats per group. The rats were fed
Procedure for collecting liver from rat
A 0.2 g sample of liver was cut to small pieces. Tissue was suspended in 2 ml of 50 mM phosphate buffer (pH 7.4), and was homogenized using a mixer at top speed for 3 min. Afterwards, the homogenate was centrifuged at 20000 g for 25 min. In this process the temperature was maintained at 4C0 during the homogenization process. Phosphate buffer was prepared based on Aebi’s method [22]. Phosphate buffer 50 mM, PH 7.0: dissolve (a) 6.81 g KH2PO4, and (b) 8.90 g Na2HPO4.2H2O in distilled water and make up to 100 ml each mix solution (a) and (b) in proporation 1:1.5 (v/v).
Enzyme activity of catalase (EC.1.11.1.6) was determined based on Aebi’s method [22]. Catalase activity was measured at 22°C by monitoring the decomposition of hydrogen peroxide. The reaction mixture consisted of 2.0 mL of the liver homogenate suspended in phosphate buffer (50 mM, pH 7.0), and 1.0 mL of hydrogen peroxide solution (30 mM). The absorbance was recorded for 2 minutes at 240 nm immediately after adding hydrogen peroxide solution. Catalase activity was expressed as moles of hydrogen peroxide reduced/min/mg protein.
Activity superoxide dismutase (EC.1.6.4.2) was assayed based on the method of Marklund and Marklund [23]. Superoxide dismutase activity was determined at 22°C by using the pyrogallol. The reaction mixture consisted of 50 mM of cacodylic acid buffer pH 8.2, containing 1mM EDTA, 300 µl of liver homogenate, 300 µl of 0.2 mM pyrogallol. The absorbance was recorded for 3 minutes at 420 nm immediately after adding the pyrogallol solution. Superoxide dismutase activity was expressed as units of SOD/minute/mg protein.
Protein concentrations were determined based on the Lowry method [24]. To 0.1 mL of sample or standard was added 0.1 mL of 2
Protein standard curve
Antioxidant is an important part of a cells defense against free radical damage. Antioxidant enzymes, in particular, constitute a major part of this defense [25]. It is evident from earlier work that different concentrations RPO have differential effects on the activities of antioxidant enzymes [10]. Figures 5, 6 and 7 showed the results of catalase activity at different concentrations of RPO (5%, 10% and 15%) for different times (2, 4 and 8 weeks) of treatment. After 2 weeks there was no significance difference (p≥0.05) between the control group and 10% and 15% concentrations of RPO while at 5% there was an increased in the catalase activity. At 4 weeks there was no significance difference (p≥0.05) between the control group and different concentrations groups (5%, 10%, and 15%) of RPO. At 8 weeks there was no significance difference between the control group and 5% group while at 10% and 15% there was decreasing of the catalase activity but there was no significance difference (p≥0.05).
The catalase activity (u/mg) in liver of rats fed with different type of red palm oil (0%, 5%, 10% and 15%) for 2 weeks. Bars are mean ± SEM (n=6), no significantly different (p>0.05).
The catalase activity (u/mg) in liver of rats fed with different type of red palm oil (0%, 5%, 10% and 15%) for 4 weeks. Bars are mean ± SEM (n=6), no significantly different (p>0.05).
The catalase activity (u/mg) in liver of rats fed with different type of red palm oil (0%, 5%, 10% and 15%) for 8 weeks. Bars are mean ± SEM (n=6), no significantly different (p>0.05).
Figures 8, 9 and 10 showed the results of SOD activity at different concentration of RPO (5%, 10% and 15%) for different times (2w, 4w and 8w) of treatment. After 2 weeks there was an increased in SOD activity at 5% while there was a decreased in SOD activity at 10% and 15% groups. However, there were no significance differences (p≥0.05) among these groups. On the contrary at 4 weeks the SOD activity increased with increasing duration of treatment in all concentrations compared to the control group. There was no significant (p≥0.05) increased in SOD activity at 15% concentration of RPO.
At 8 weeks there was no significant difference (p≥0.05) between the control group and all treatment groups of RPO except there was decreased in SOD activity at 15% of RPO. Therefore, after 4 weeks the activity of SOD was significantly higher (p≤0.05) at 15% of RPO dietary group compared to the control group but the increase in the 10% of RPO dietary group was not statistically significant. On the other hand, there was a significantly decreased (p<0.05) in 15% of RPO dietary group after 2 and 8 weeks.
The results of this study showed that 15% treatment of RPO which contain β-carotene and vitamin E for 4 weeks may enhance the antioxidant enzyme (SOD) defence system. These results thus suggest that a combination of carotenoids and vitamin E (tocopherol and tocotrinol) in the RPO has an important role in the protection against free radical damage. Red palm oil contains the highest concentration of tocotrienols compared to other vegetables or plants and the tocotrienols can be 40-60 times more potent as anti-oxidant than tocopherols [26]. Tocotrienols are free radical scavenging antioxidants, however, only the α-isomer has considerable biological antioxidant activity. It is therefore not surprising that there are relatively very few studies on their antioxidative effects in oils and fats [2, 26].
Although a few of studies explicitly show the effects of vitamin E on the activities of antioxidant enzymes, there is no consensus on what might be the responses of antioxidant enzymes to vitamin E, partly because of different feeding behavior and other ecological conditions [27].
The superoxide dismutase (SOD) activity (u/mg) in rat liver fed with different type of red palm oil (0%, 5%, 10% and 15%) for 2 weeks. Bars are mean ± SEM (n=6), no significantly different (p>0.05) at all treated groups with RPO.
The superoxide dismutase (SOD) activity (u/mg) in rat liver fed with different type of red palm oil (0%, 5%, 10% and 15%) for 4 weeks. Bars are mean ± SEM (n=6), no significantly different (p>0.05) at 5% and 10%, significantly different (p<0.05) at 15%
The superoxide dismutase (SOD) activity (u/mg) in rat liver fed with different type of red palm oil (0%, 5%, 10% and 15%) for 8 weeks. Bars are mean ± SEM (n=6), no significantly different (p≥0.05).
The results of CAT activity at different vegetable oils (RPO, PO, CO and COC) for different times (4 and 8 weeks) of treatment are summarized in Figures 11 and 12. After 4 weeks there was no significance different (p≥0.05) between control group and different vegetable oils treated groups while at 8 weeks there was significance decreased (p≤0.05) in PO,CO and COC groups compared to control group but the CAT liver sample was no significant different (P≥0.05) between control group and RPO group.
Several studies have illustrated that RPO is a rich cocktail of lipid-soluble antioxidants such as carotenoids (α- and β-carotene, lycopenes), vitamin E (in the form of α-, β-, δ- tocotrienols and tocopherol) [27]. Red palm oil has 17,500 mg of β-carotene per 100 g, and 28,000 mg of α-carotene per 100 g for a total of 6,140 retinol equivalents per 100 g. Thus, it has good potential for routine diets with enrichment carotenoids [28]. Red palm fruit oil (RPO) contains about 15 times more carotenes than that present in the same weight of carrots, and 44 times that of leafy vegetables [29]. Palm oil is a rich source of vitamin E, having both tocotrienols and tocopherols [30, 31].
The catalase activity (CAT) in rat liver fed with different vegetable oils for 4 weeks. Bars are mean ± SEM (n=6), no significantly different (p≥0.05).
The catalase activity (CAT) in rat liver fed with different vegetable oils for 8 weeks. Bars are mean ± SEM (n=6), different alphabet an each bar indicate significant different (P≤0.05).
The results of SOD activity at different vegetable oils (RPO, PO, CO and COC) for different times (4 and 8 weeks) of treatment are summarized in Figures 13 and 14. After 4 and 8 weeks there was no significance different (p≥0.05) between control group and different vegetable oils treated groups.
The superoxide dismutase (SOD) activity in rat liver fed with different vegetable oils for 4 weeks. Bars are mean ± SEM (n=6), no significantly different (p≥0.05).
Mean superoxide dismutase (SOD) activity in rat liver fed with different vegetable oils for 8 weeks. Bars are mean ± SEM (n=6), no significantly different (p≥0.05).
The results from the present study, after different times, showed that under sedentary conditions,
This study finding was similar to that of Rathnagiri et al. [33] who reported that there were no statistically significant differences between control fed rats with respect to SOD activity in the corn oil. The effect of COC on antioxidant enzyme in this study was not in agreement with Anitha and Lokesh [34] who found that COC increased significantly of SOD activity in the liver but Anitha and Lokesh [34] used coconut oil with groundnut oil or olive oil instead of COC. Vitamins directly scavenge ROS and regulate the activities of antioxidant enzymes. Among them, vitamin E has been recognized as one of the most important antioxidants [27].
This probably involves their actions as antioxidants, reducing the level of free radicals and hence free radical damage. Antioxidant enzymes, such as superoxide dismutase (SOD) play a major role in removing the Reactive Oxygen Species (ROS) [11]. At this time point, it is suggested that different experimental period might lead to different result about the effect of dietary vitamin E on the activities of antioxidant enzymes [27]. In the present study, the 2 weeks period in which this experiment was carried out may be insufficient to witness any change in the activity of this enzyme.
In addition to this, Yazar and Tras [35] reported that prior induction of ROS could cause an increase intracellular SOD activity. Hence first induction of ROS may cause changes in SOD activity and then SOD activity may return to the normal level. SOD enzyme, together with CAT, protects cells against damage caused by free radicals and hydrorlipoperoxides [36]. According to Catherine et al. [37] vitamin E work synergistically to decrease the multiplication of free radicals. Vitamin E inhibits the production of lipid hydroperoxide. However, reduced SOD activities may also indicate increased lipid peroxidation end-products like acid thiobarbituric [38]. Intricately linked to lipid peroxidation are antioxidant enzymes such as SOD and catalase. As a defense against reactive free radicals, the body produces antioxidant enzymes which help to mop them up [39].
As red palm olein was shown to reduce MDA production and increase SOD in 15% group for 4 weeks, it would spare the retina from damage. This effect of palm oil may be related to the ability of β-carotene to quench free radicals and prevent tissue damage [40]. The relatively lower cholesterol level in treated rats and higher antioxidant enzyme activity could be viewed as potentially beneficial for the health of the user population in humans [41].
Presence of high amount of unsaturated fatty acids may be the reason for the low antioxidant enzyme activities of some vegetable oils fed rat since polyunsaturated fatty acids (PUFA) deteriorates the antioxidant status due to their liability to become highly oxidized. Feeding oils high in polyunsaturated fatty acids (PUFA) results and increase the oxidative stress since PUFA are highly susceptible to peroxidation than monounsaturated and saturated fatty acid [42].
A) Stressed control group, (B) Stressed red palm olein group, (C) Stressed palm olein group
Figure 16 shows the results of CAT activity in liver samples of normal and stressed rats that were treated with 15% of RPO and PO for 4 weeks of treatment. After 4 weeks, there was no significant difference (P≥0.05) between control group and 15% RPO and PO normal groups whereas there was significant decreased (P≤0.05) between control group and 15% RPO stressed group and there was significantly higher (P≤0.05) in 15% PO stressed group than the control group. This study finding were similar to that of Benson and Kshama [7] who reported that the CAT activity in RPO group has shown significant decrease compared to PO and RPO groups under stress conditions.
Many recent studies emphasize the important role of reactive oxygen species (ROS) in the pathogenesis of various liver diseases. Stress known to increase oxidative stress in the major organs including the liver [42].
The catalase (CAT) activity in normal and stressed rats fed with red palm olein and palm olein for 4 weeks. Bars are mean ±SEM (n=6), different alphabet an each bar indicate significant different (P≤0.05).
Figure 17 shows the results of SOD activity in liver samples of normal and stressed rats that were treated with 15% of RPO and PO for 4 weeks of treatment. After 4 weeks, there was significantly lower (P≤0.05) in 15% RPO and PO normal and stressed groups than the control group. Vitamin E is a major antioxidant vitamins found in the cell and can prevent cell damage through its activity as a free radical chain breaker [43]. Free radicals have been implicated in the etiology of large number of major diseases. They can adversely alter many crucial biological molecules leading to loss of form and function. Such undesirable changes in the body can lead to diseased conditions. Antioxidants can protect against the damage induced by free radicals acting at various levels [43].
β-Carotene has received considerable attention in recent times as a putative chain-breaking biological antioxidant and its ability to interact with free radicals such as peroxyl radicals and to scavenge and quench singlet oxygen is well documented [44]. Defense mechanisms against free radical-induced oxidative damage include the catalytic removal of free radicals and reactive species by factors such as catalase (CAT), superoxide dismutase (SOD) and reduction of free radicals by electron donors, Such as vitamine E (tocopherol and tocotrienol) [45].
The superoxide dismutase (SOD) in normal and stressed rats fed with red palm olein and palm olein for 4 weeks. Bars are mean ±SEM (n=6), different alphabet an each bar indicate significant different (P≤0.05).
In conclusion, palm oil may offer some protection to liver of the treated rats by reducing free radicals damage, as well as increasing SOD. The present study shows no significant difference in level of catalase in control group and different concentration groups of RPO treatment but after 4 weeks 15% of RPO was enhanced the SOD activity level in rat liver. It can be concluded that the effect of different concentrations of RPO appear to depend on the different period of treatment. The current study shows no significant difference in level of catalase in control group and RPO group but the treated rat liver with PO, CO and COC groups were the lowest and it were significantly lower than control group. After 4 weeks of treatment, 15% of RPO enhances the SOD activity level in rat liver.These results could be due to the high content of vitamin E (tocopherols and tocotrienols) and β-carotene in red palm olein. Treatment with 15% RPO and PO diets did not affect the CAT activity after 4 weeks of treatment under normal condition while there was decreased in CAT activity with RPO and increased with PO under stress conditions. Additionally, the results in RPO group showed that higher SOD activity compared to PO and control groups under normal conditions while there were no significant difference (P≤0.05) in SOD between the control group and treated groups under stress conditions.
This work was supported by the Organization for Women in Science for the Developing World (OWSDW) and the research was funded by UKM-GUP-NBT-27-103, UKM-HEJIM-Industri-16-2010 and UKM OUP-NBT-29-139/2011 We grateful thank to caroteno sdn bhd Malaysia for providing red palm olein sample
In 1948, the World Health Organization (WHO) defined health as ‘a state of complete physical, mental, and social well-being and not merely the absence of disease or infirmity’. WHO supports the idea about the integration of conventional and complementary practices to reach the best results for the patient and society [1] Traditional, Integrative, and Complementary Medicine practices are being adopted in several countries’ members of WHO. Acupuncture was the most common form of practice, closely followed by herbal medicines and indigenous traditional medicine. Homeopathy and traditional Chinese medicine came in next, each used by 100 Member States [2].
In 2006, Brazil the Health Ministry launched the National Policy of Integrative and Complementary Practices (PNPIC, MS ruling no. 971/2006) [3] with ensures to the population partial access to Complementary and Alternative Medicine. Under PNPIC, patients are provided with traditional Chinese medicine/acupuncture, homeopathy, botanicals, and herbal medicine cares free-of-charge, among others at Health Basic Units (HBU) and Family Health Support Units (NASF). Yoga was included in 2017 [4], and finally, apitherapy with the other 9 practices was included in 2018 [5]. According to the Health Ministry from Brazil, in 2019, 1,4 million of individual queries were done in these practices, with acupuncture in the leadership. Integrative practices are present in 9350 services and 3173 cities, from this 88% of the public health care system.
Considered the last frontier to be crossed in Hippocratic medicine, vibrational medicine aims to shed light on the understanding of the bodily system in its most subtle aspect of energy transference and systemic functioning regarding the physical-etheric interface of bodies. The Cartesian and mechanistic division of science and the world of the 20th century, which undoubtedly drove the great technological advance that the world finds today, as postulated by Einstein, Heisenberg, and colleagues, overcame this Newtonian fragmentation and led us back to the idea of unity, expressed in ancient Greece and Eastern philosophies [6].
Albert Einstein was one of the greatest physicists in recent history and, from him, there was the recognition that all matter is energy, and one can assume the understanding that the human body is a dynamic energy system. Energy and matter are two different manifestations of the same universal substance from which we are all formed, atoms. It is known that the movement between the constituent parts of the atom (electrons, neutrons, protons and smaller particles) forces that lead them, to generate energy. As the atoms are in constant motion, what can be measured is vibration. Each atom is unique because the distribution of positive and negative charges, combined with the speed of rotation, creates a specific vibration and a personalized frequency pattern [7]. The biophysical aspects of some therapies such as acupuncture, yoga, homeopathy among others, in general, are not many in publications in the area, due to the limited instrumental validation available.
The electromagnetic force is the fundamental reason for life. Atoms are sets of electrons and protons, molecules are sets of atoms, biopolymers are groups of macromolecules, and in the same way, life is an interactive congregation of biopolymers and macromolecules. The living being can then be considered an electromagnetic entity, which responds to a given electric or magnetic signal, as an expected result based on the laws of physics. Experiments have shown a level of electromagnetic organization in living organisms; intrinsic electrical characteristics and their sensitivity to external electromagnetic fields [8]. In this way, the human body can be considered a “resonance box”, capable of responding to various sources of stimuli (mechanical, sound, light, olfactory and tactile). Thus, everything that acts in its electromagnetic field or that comes into contact in the form of vibration in its physiological ensemble is liable to mobilize and be transformed into energy. It is from understanding the connection between the subatomic universe and physiology that we can understand how acupuncture, yoga, homeopathy, and why not apitherapy, that are capable of triggering various biochemical activities already mapped by science with their purely vibrational aspect. Considering the importance of the integrative and complementary medicine for health and aiming to contribute to the data already available in the scientific literature, this chapter aims to present some concepts about acupuncture, yoga, homeopathy, and apitherapy from the vibrational point of view, an important approach to understand the functioning of these impacting traditional medicines in human life.
Acupuncture, one of the tools of Traditional Chinese Medicine (TCM), in its more than 3000 years of existence, appeared intuitively in China, however it has had progressive scientific validation since the middle of the 18th century and, until today, it proposes to do the integration of the physical, psychic and spiritual bodies through the management of vital energy (
According to Lipton, the behavior of energy waves is important for biomedicine because vibrational frequencies can change the chemical and physical properties of an atom [11]. The knowledge of the links between the material body and its subtle form can enable a greater understanding of how it is possible to expand health in a preventive way in a global, affordable, and personalized environment [12]. We will not stick to the descriptions of the meridians paths, treatments, or explanation of the syndromes that are treated by acupuncture, but the biophysical events as a link that provides the effectiveness of the treatments of this ancient method.
According to Chinese philosophical thought, there is a concept of energy that permeates everything around us. It influences from the simplest form of life and even the movement of the planets, in a constant flux of renewal and expansion. This force/energy is part of the essence of the universe and involves all the entities that exist in it are called TAO [13]. In this philosophy there is a very particular view that correlates all the movements of the universe, macrocosm with the microcosm that represents everyone, in this way, everything is part of a great set. In this logic, the body is a macrocosm with countless microcosms, transforming and expanding with each movement of the individual. With this position, when contemplating a garden or a beautiful landscape, human being knows that the same energy that sustains and nourishes nature also exists within him; it manages to amplify its capacity for self-understanding and to value the importance of life cycles, confirms that it can establish itself in the face of the elements of existence [14]. From this point of view, the individual relates better and more respectfully to everything around him, perceives and contemplates the beauty of cycles, such as the arrival and the end of a season, harmoniously moving creation and destruction, and above all, he perceives himself to be actively acting in a macrocosm full of microcosms. Energy flows can be compared to the movement of the oceans. The currents have different characteristics, one is hot and fast, the other cold and slow, they vary according to the depth, and when they move they generate a lot of energy in this ecosystem [6].
In the millennial Huang Ti Nei Jing, known as Principles of Internal Medicine of the Yellow Emperor, we find the following statement: “the whole universe is an oscillation of
For the quality of life and health maintenance, acupuncture treatments provide information for understanding the circulation of vital energy (
In Oriental philosophy, the absence of health is seen as a momentary resource of the body in the search to reorganize itself, being a system of adaptation of this organism to anomalous environmental stimuli, pollutants, toxic agents, conflicts, and a decrease in vital energy.
The oldest written reference on acupuncture meridians is present in the book Huang Ti Nei Jing and contains precise descriptions of its principles and we can assume that it is the result of observing the beginnings of Chinese medicine. It contains information that, when stimulating the points on the map associated with certain organs or viscera (
Exploring the developments resulting from the relationships between them is not the goal of this chapter but understanding how the dermatomes connect with the meridians serves to complete the understanding of the invisible systemic tracks that run through the organism. To understand the vibrational vision in acupuncture, and which supports it scientifically, the first step is to know the studies that can validate this specialty over time, through the embryogenesis of acupuncture meridians.
It is known that meridians are a distinct morphological pathway. This finding was possible in humans through the findings of Frenchman Pierre de Vernejoul and his collaborators, who injecting metastable radioactive technetium 99 (99mTC) into the acupuncture points, observed the progression of the isotope in the mapped meridian lines, covering a distance of 30 cm in 4 to 6 minutes. When 99mTC was injected at random points on the skin, there was no similar result compared to the injection in the cuff [10].
Vernejoul based the work of Korean Kim Bong Han in the 1960s. By visualizing the path of the isotope phosphorus 32 (P32) injected into a rabbit’s acupuncture point, Kim observed the absorption of the isotope in a tubular system with about 0,5 to 1.5 μ (microns) in diameter with the use of micro auto-radiography; this path corresponded to the acupuncture meridian tracing of a given point; little or no activity was noticed in the meridional line when the isotope was injected into a vessel adjacent to the point. Through the histological study of these tubules, Kim’s group found that there were superficial and deep ducts that “floats” freely over the inside of the lymphatic vessels and vascular tissue, penetrating the vessel walls at specific points of entry and exit. The fluid present in these tubules moved in the same direction of blood and lymph flow and also in the opposite direction to them, which suggests that its origin is probably independent and previous chronologically to the embryogenesis of blood and lymphatic vessels.
According to him, as long as blood vessels develop, they grow around the meridians. The sequence of these findings was followed by the description of other tubular systems: The Intra-external Ducts, which appear to be arranged along the surface of Organs internal organs and flow independently from the blood, lymph, and nervous vessels. Superficial Ducts are found on the skin; and, finally, the Neural Ducts, distributed in the central and peripheral nervous system. All Duct systems are interconnected by Terminal Ducts of the various ductal systems and these reach the nucleus of the cell. Kim has also done numerous experiments that confirm the continuous flow of these ducts and the fluid contained in them. Within these ducts were found high concentrations of DNA, RNA, amino acids, nucleic acids, sixteen types of free nucleotides, adrenaline, corticosteroids, estrogen, and other hormonal substances at levels different from those found in the bloodstream. This indicated the path of understanding the interrelationship of acupuncture meridians and the regulation of the endocrine system [10]. Kim’s findings, according to Richard Gerber (2007), were associated with those of Yale’s neuroanatomist Harold S. Burr. Burr deeply studied the energy fields surrounding plants and living animals. He mapped the electric fields in the salamanders at an early stage of embryogenesis and found that the electrical axis that was aligned with the animal’s brain and spine originated in the unfertilized egg. Kim, consulting Dr. Burr’s findings, found that in the chicken embryo, the meridian ducts formed within 15 hours of fertilization. At that time, not even the most rudimentary organs were formed. With this, he can suggest that the functioning of the acupuncture meridian system influences the migration and the spatial orientation of internal organs. Burr and Kim proposed that meridians form a physical-etheric interface, where bioenergetic information and vital energy flow from the etheric body to the cellular level through these tracks, nourishing the bodily systems [10].
We can connect these studies with those of molecular biologist Bruce Lipton (2007), who, through a quantum perspective, reveals that the universe is a set of integrated and interdependent energy fields. The physical part and the energy fields that make up matter describes a non-linear or holistic flow of permanent emanation. According to him, the specific frequencies and patterns of electromagnetic radiation in the environment can regulate DNA, RNA, protein synthesis, alter the function and shape of proteins, control genes, cell division, their differentiation, morphogenesis (a process in which cells group together to form organs and tissues), hormonal secretion, growth and nerve functions [8]. In acupuncture treatment, the insertion of needles into acupoints, known in Chinese literature as the arrival of the sensation of
We have already seen that the meridians are not like a vascular system and neither are nerves, but they can be the neural and neurohormonal links between the central nervous system (CNS) and the peripheral nervous system, influencing it through the connective tissue when stimulated through their paths. In the nervous system, communication takes place through electrical action potentials. Information is transmitted through changes in the frequencies of action potential discharges. The rate of nerve electrical discharge per second generates a code, which will have a certain reading depending on the nerve that is communicating with the brain sensory region. The discovery that the systems made up of glial and Schwann cells - which previously only served to nourish the surrounding nerves also has the function of an electrical nature [10], is an important link for understanding this communication system physical-etheric. The most recent research indicates that the glial cell network can transmit information through slow changes in direct current potentials. It is possible that with the vibration of acupoint stimulation, which is characterized by a location on the skin with low electrical potential, an input is created in the nervous system, influencing the direct-current potentials of the glial cell network, which follows the path of the nerves [10]. When an action potential discharge begins in a nerve cell, a sequence of events is triggered, which passes through the entire sensory nerve fiber until reaching its synaptic ends. The electrical impulse, which carries a message to the brain, undergoes an energetic transformation in the synaptic cleft, which is converted into the release of neurotransmitters. The electrical potential in the cell membrane determines the reaction of each neuron to release neurotransmitter packages. Each nerve cell is in contact with many others forming a network, thus “spreading” the information for the modulation of the CNS and, consequently, the harmonization of regions that are unbalanced in the body system. It can be assumed in this way that the dual wave-particle behavior of subatomic particles is producing a set of “information” that, in essence, generates vibrations (waves) that seem to make the connection between the material and immaterial systems of bodies [12]. The acupuncture meridians and the nervous system operate in a complementary manner and, when adequately supplied with information and nutrition, they will promote higher energetic phenomena translated into harmonic cellular physiological patterns and also immunity, organized by the individual and unequivocal model of functioning present in each individual in the pituitary.
With the change in the energy environment of glial cells, the meridian system becomes capable of influencing the bioelectronic systems of growth and regeneration. We conclude with this that the effects of neurochemical releases, associated with changes in direct currents that are slowly transmitted along the perineural pathways, are not primary, but a secondary effect of the fluctuation of energy fields located in the vicinity of nerves and glial cells. Surrounding them from the vibrational stimulation of the needles in the dermis.
It is common knowledge that vibration triggers the action potential (AP) in neural cells, which originates through a disturbance of the resting state of the cellular membrane, with a consequent flow of ions, through the membrane and alteration of the ionic concentration intracellular and extracellular media [18]. Thus, the main cause of the resting potential would be the unequal distribution of ions in solution on both sides of the membrane, compartmented actively or passively by the selective mechanisms of transmembrane ion transport. The membrane, therefore, acts with a capacitor, storing energy in this spatial distribution of electrically charged ions; this potential electrical energy is available to be recovered quickly, in addition to stabilizing the membrane preventing this system from being disturbed by any minor factor. The aqueous medium fills most of the intracellular and extracellular spaces, and it is where almost all molecules (soluble, of course) interact to animate the intermediate metabolism, the mobilization of energy and nutrient sources, and the maintenance processes are suspended, and molecular and cellular repair [19]. The important fact is that all living cells have some differences in electrical potential between the cytoplasm and the extracellular space, being generally negative on the inside (resting potential of the cells). Some cells, however, can leave this situation of rest, propagating, throughout their membranes, disturbances that cause transmembrane ionic currents throughout the cell, and that can even invert the electrical profile concerning rest, even leaving it for some time the cytoplasm positive concerning the exterior: these are called excitable cells, and include neurons, muscle cells, and endocrine secreting cells. Some of these nerve endings release outgoing transmitters that tend to trigger an impulse; others are inhibitors and reduce the nerve’s tendency to fire. The impulse trigger will depend on the balance between the exciting and inhibiting influences of hundreds of synapses [15]. This mechanism and all known developments in the Western are responsible for the vibrational potential produced by acupuncture (Figure 1).
Neuroendocrine modulation from acupuncture stimulus (Source: Gerber, 2007).
The trigger above explains the
It is in the transformation of
The greater the understanding of the biophysical aspects of the energy generation, information, nutrition, and defense production processes in the body system, the better and more comprehensive the direction of integrative and complementary therapies in current and future integrative medicine will be.
Yoga is one of the six most important philosophies in India and to understand it from a vibrational point of view, first we will present some important concepts. As well as several other holistic knowledges, there are indications that Yoga has existed for at least 3000 years. It is a timeless philosophy that comprises a set of moral and ethical values, as well as some techniques to harmonize body, mind, and spirit. And although it is based on teachings contained in the “Vedas”, texts that form the basis of Hinduism, they have no religious connection. In Sanskrit, a language considered sacred in India, Yoga means to “unite”. It refers to the union of the being with the whole, through the study of oneself (self-knowledge) and the understanding that mind, body, and spirit do not separate.
For centuries, the teachings of Yoga were passed on orally, directly from master to disciple. Until, around 200 BC, a great Indian philosopher, “Patañjali”, codified them in 196 aphorisms, constituting what is known today as one of the most important texts of Yoga, the “Yoga Sutras” [20]. It should be noted that among these aphorisms, two of them, the III-53 and IV-33 deal with theories where the nature of time and matter would be discontinued, in line with current discoveries of quantum physics, scientifically proven, that irradiation of light is not continuous [21]. Patañjali describes eight steps that the Yoga practitioner must follow. The first two stages,
The “Yogis” believe that the physical body is only one of the five different bodies (layers), which constitute the being and that must be in perfect harmony to reach the state of totality. The five bodies, or
According to this philosophy, there is an energy originated in the Sun, which penetrates the Earth’s atmosphere through the sun’s rays and is responsible for all life on the planet. This energy is called Prana and has been reported by different peoples around the world for millennia. They gave these energy different names, but it is the same vital energy. In China, it is known as
The sacred texts also speak of 72,000
The analogy between these two different structures, one based on neurophysiology and the other on a tradition of ancestral wisdom, has been the focus of several scientific studies. They aim to understand how this complex and extensive energy network is related to the nervous system, endocrine glands and blood and its relationship with the health-disease process [27, 28, 29, 30, 31, 32].
Neuropsychiatrist S. Karagulla deepened her research on the interaction of the etheric body and
It is also worth mentioning the research carried out by Motoyama, Ph.D. in Philosophy and Clinical Psychology, which tends to confirm the existence of
Another relevant study was carried out by Dr. Valerie Hunt, which were used electrodes electromyography devices connected to telemetry equipment, to capture changes in the skin, in the region corresponding to the location of the
According to the Yoga philosophy, the principle of everything that exists in the universe is propagated through vibrations. Thus, it is believed that thoughts, words, feelings, food, and everything else that exists on this planet, has a vibratory pattern that can affect people’s health in a positive or negative way.
The balance between the five bodies and the healthy functioning of the
Recently, scientific studies have been published to assess the benefits of
The vibrational benefits of Yoga are also promoted by chanting
Another technique widely used in Yoga, are the
The five fingers of the hands represent the five elements. Fire (
There are several reports on the “powers” acquired through the practice of Yoga. Motoyama recounts his experiences in the book “Theory of the
However, Yoga is a philosophy that uses techniques to manipulate and harmonize vital energies, unlock the channels and energy centers, encouraging the practitioner to (re)connect with the vibration that exists in everything. Induces the practitioner to disconnect from the external agitation and dive into the internal vibration, becoming aware of the energetic reality emerging in their bodies and minds, and thus, letting go of
Homeopathic therapy was created by the German physician Samuel Hahnemann in 1796, due to his dissatisfaction with the therapy used at that time. Hahnemann made his proposal for this new therapy after seeing that the symptomatic picture of malaria, which had quina bark (
This concept had already been presented by Hippocrates, considered the father of Medicine, who describes that “the disease is produced by the similar and, through the similar, the patient returns to health”. Although this proposal was previously presented, it was Hahnemann who was responsible for demonstrating it clinically, based on his fundamentals, endowed with information in the Experimental Medical Matter and with an exclusive Pharmacotechnics [47].
In addition to the presented principle that “the similar heals other similar”, Hahnemann introduced the concept of minimum doses, explaining that these were chosen after their effectiveness was noticed, since, by reducing the concentration of substances through their dilution, the same continued to have a therapeutic effect, without harming the symptoms of intoxication [48].
The art of studying the toxic effect of substances on animals already existed, but it was necessary to make a similar study in man, so that Hahnemann developed this experimentation, elaborating the homeopathic medical materials from these observations. To explain this fundament, the father of Homeopathy quotes in his book considered being the greatest work [49],
Another basis of homeopathy presents the proposal of using a single medicine, capable of covering the entire symptomatic of the individual, which is, a medicine whose pathogenesis best coincides with the manifestations presented by the patient, this being his simillimum [47], that is, a medicine that when used, will act considering the organism as a whole, having all its integral parts, in order to re-establish the harmony of the system.
From his experiences, Hahnemann proposed a therapy based on the vital force, explained by him in the 6th edition of Organon [50] by the words
And then, according to the principle of Vital Force, under the Law of Similars proposal, and following the fundamentals of experimentation in a healthy man, minimum doses and single medicine, it was published by Hahnemann, in 1976, in the Journal of Practical Medicine, an article in which states that “disease can be cured by drugs that cause symptoms similar to the disease” [47].
To exemplify the law of similars, Bellavite et al. [49], presented some schematic representations of two examples of the application of the law of similars and of diluted and dynamized substances, in inflammatory models. The example presented was obtained with the medicine
The scheme presented represents the results of Poitevin et al. [50] and were based on the use of bee venom (
As shown in Figure 2a and b, the sources of the medical material
a - Activation of normal basophil degranulation caused by IgE antibodies. b - Inhibition of basophil degranulation by the use of homeopathic drugs (ultra-diluted and dynamized) such as
The mechanisms by which Hahnemann’s proposal is based is not yet clearly demonstrated, and most likely due to the technological limitation for such energetic measures involved to be made. A look from a vibrational point of view may shed light on a possible way in which homeopathic medicines act, especially when you think about the concept of a single medicine and its fundaments that the similar heals other similar.
Homeopathic medicines come from a solution or mother tincture produced from products of plant, animal, or mineral origin. All of these have atoms, protons, electrons, neutrons and smallest particles, and for that reason, they are in constant vibration, and the energy/matter duality, just like humans, having the premise of Einstein’s theory, in which matter is composed of particles, which ultimately consist on light/energy, in the wave/particle duality. Analyzing the Hahnemannian point of view on the vital energy, someone could suggest that the vital energy is the vibration that needs to be preponderant for the state of health. When diseases change this vibrational frequency, they need something that offers the dosage of energy/frequency necessary for this vibration return the original vital energy.
In the process of health and illness, it could be said that the individual’s energetic/vibrational state has been altered, and in order to restore the original energy, a vibrational energy medication compatible with that necessary for the restoration of the individual’s vital energy, is necessary. Thus, and taking as a reference the homeopathic process of dynamization of the medicines, which follows a dilution and a succussion (rhythmic shaking of the bottle containing a medicine), aiming to awaken the medicinal energy of the substance, in water, and considering that the raw materials of departure have their own vibration, it is possible to suggest that homeopathic medicines, already without quantifiable matter by chemical methods, considering the number of Avogadro, have only the vibration of the original substance, the subtle energy, which will exercise its function of the ethereal subtle energy of the sick individual.
One hypothesis is that the drug energy of a substance that causes a symptomatic picture identical to that provoked by the disease, presents the same frequency as it, and thus, the frequencies cancel each other out, returning the vibration of the normal individual to preponderate. It is not uncommon in the homeopathic healing process, the individual presents an aggravation of the symptoms, and this could be the consequence of the initial summation effect of the medication vibration, added to the energetic picture of the disease, which highlights the symptomatic picture to stimulate the immune response to restore the individual’s energetic/vibrational state of balance.
After the Albert Einstein presentation about the revolutionary theory of energy and matter, using the very famous equation: E = mc2, which establishes the quantitative equivalence of the matter’s transformation into energy or vice versa, and the comprehension of each atom is composed by electrons, neutrons and protons that are under constant vibration, emitting energies with different frequencies, and that humans are, in the last level, composed by atoms (equivalent to energy), it is much easier to understand that each individual possesses your own vibrational standards. When this vibrational standard state of ‘normal’ level is modified by one disease, something needs to happen to re-establish the energy to the ‘normal level’ again (Vital Energy,
To clarify this statement, imagine the electrons that are in specific space regions known as orbitals. Each orbital presents a specific characteristic of energy and frequency, according to the atom and its molecular weight. In order to change the position of one electron to another next superior orbital, it is necessary to transmit the energy of a certain frequency. Only one quantum of exact energy necessary will stimulate the jump of the electron to the next superior orbital. Through the resonance process, the energy of appropriate frequency will excite the electron and cause it to pass to the highest energy level in its orbit around the nucleus. Maybe human behavior could be compared to the electron change of energy level, if we consider (as an analogy) the existence of ‘orbitals’ of health and disease, for example with different vibrational levels (vibration/frequency). In the case of human beings whose energetic systems are in a disease orbit, only a subtle energy dose with the appropriate frequency can be accepted and cause the body to move into a new orbit or steady state of health. Vibrational remedies could inject the quantity necessary of subtle energy in the human system through the induction of resonance. This etheric energy changes the vibrational level of disease to the orbital of health [10].
The energy that gives life is called ‘vital energy’ in homeopathy, ‘prana’ in yoga and
During this chapter, it was possible to present until now, 3 different types of treatments, all based on vibrational medicine, with different techniques: acupuncture, yoga, and homeopathy. Considering all this information, now it is time to talk about apitherapy and how bees can help during the health process.
Apitherapy or “bee therapy”, from Latin
Ancient civilizations with their millennial therapies recognize and use the bee products as valuable resources in their medical practices. The history of medicines of the Chinese, Tibetan, Egyptian and also the Greco-Roman civilizations are quite rich, containing in its records, dated for a long time (more than 6000 years in ancient Egypt), hundreds of recipes, including among the main ingredients, honey, propolis, bee larvae and eventually the bees themselves to cure or prevent diseases [53].
Hippocrates (460–370 BC), Aristotle (384–332 BC), and Galen (130–200 AD) prescribed the use of honey and bee venom as a cure for baldness. Hippocrates recognized the healing virtues of bee venom for treating arthritis and other joint problems, also used propolis for healing sores and ulcers internally and externally. Today growing scientific evidence suggests that various bee products promote healing by improving circulation, decreasing inflammation, and stimulating a healthy immune response [51].
Nowadays, apitherapy is adopted in some countries as Complementary and Integrative Medicine with a specific regulation, as is the case of Brazil for example [5], but some others deserve special attention since they possess an important tradition in the apitherapy use and/or Organizations of Apitherapy running some job in each country in this field. In Brazil, honey and propolis possess widespread use by the population. Apis Flora Company introduced propolis extract in the Brazilian market at the beginning of the 80s, and from this moment, several other innovations appeared as the natural syrups based on mixtures of honey with propolis and others bee products, and/or with herbal extract and/or essential oils, vaporizers with the same type of mixtures. Extracts, lozenges, tablets, capsules, and others can be found in European, American and Asiatic countries, and they can be found in pharmacies, drugstores, natural product houses, supermarkets, etc. [54]. Besides honey and propolis, royal jelly, bee pollen, and apitoxin can also be found, however with less expression than the firsts.
For understanding the apitherapy importance as integrative medicine and in order to propose some connection with vibrational medicines, it could be useful to present some small information about what each bee product is, its traditional use, composition, and benefits according to clinical trial and/or systematic review available until now. As the literature about each bee product is quite large, and the deeper description about “in vitro” and “in vivo” protocols about mechanisms involved already done, are not our focus in this chapter, only some information will be provided in order to try to propose an innovative way about thinking apitherapy with vibrational medicine information. But first, let’s know something about the vibrational communication between bees.
As could be seen in the text published by Scheider & Lewis [55] the vibration signal produced by bees is used as a modulatory communication signal, as it is involved in several actions executed in the beehive. The frequencies, high or low, the pulses short or long, besides other characteristics in the vibration not only activate but also are involved in the stop of some functions. Von Frisch [56] compared this warning sounds with the vibrations produced by honeybees during “buzzing dances” and the buzzing tones. Stop signals are short, high-frequency signals that occur under rather different conditions. Nieh [57] described that short pulses of body vibrations were performed by foraging bees, offering a warning to the others about a dangerous food experience. The literature available proposes that different signals communicate different things, activating, or stopping actions. Not surprisingly, many different terms have been used to characterize these different short pulses (e.g., piping, begging, buzzing, shaking, whooping).
Besides the communication actions developed, Schneider, Huang & Lewis discovered that the juvenile hormone titers of workers 15–30 min after receiving vibration signals are slightly, but significantly higher than those of non-vibrated control bees that are matched for age, location in the nest and initial activity levels [55]. One parallel that it is possible to do in this topic is that the evidence already described the higher concentrations of some hormones like adrenalin, estrogens, corticosteroids, besides others, in the meridians used as key points in acupuncture, in comparison with the concentration in the blood, show evidence about the connection of these points with the body endocrines glands [10]. So, the vibration modified by the application with the needles in the acupuncture in these meridians can modify the endocrine glands’ behavior, affecting some physical behaviors in the body. Here it is possible to meet some convergence with what vibration provoked by bees can do in the production of some hormones of other bees.
It let us consider that vibration produced by bees can stimulate higher production of some compounds in the bee organism. And the question that one could do is: Can vibration produced by bees energize bee products produced in the beehive as royal jelly, bee venom, honey, propolis, etc.? Is there some characteristic vibration of bee products that can affect the etheric vibration of the humans? Of course, this hypothesis needs to be validated, but let’s do an exercise with bee products in order to evaluate this hypothesis.
Honey has been used in the human diet since 25,000 years ago when the first tangible demonstration was found [58]. Besides its use for nutrition and mainly as a sweetener, its use as a traditional medicine in several cultures worldwide was very well documented [59, 60, 61]. The use of honey for therapeutic purposes dates back to Egyptian papyrus, and it was also mentioned in Chinese medicine and Hindu documents [62].
The chemical composition of honey is based on a supersaturated sugar solution, especially rich in fructose and glucose, which also present more than 181 substances containing minor quantities of minerals, vitamins, phenolic acids, flavonoids, enzymes, organic and amino acids, proteins, and a huge variety of essential oils according to the different flowers visited by honey bees [63, 64]. The honey composition will never be the same since it depends on the source-type, soil, climate, and genetic factors and procedures methods involved. In this sense, the feeling and sensation promoted by honey as colors, flavors, smells, and tastes difficult will be the same [65, 66].
Several biological properties were already demonstrated for honey as antioxidant action that contributes to the prevention of several diseases as cardiovascular problems, cancer, diabetes, and others, especially by the protection against free radicals and other oxidative substances [67, 68, 69]. Besides antioxidant action, antimicrobial and wound healing are other two properties very well recognized in the literature and it is mainly attributed to high osmolality and sugar concentration, low pH, aromatic acid, volatile substances, and peroxides composition [70, 71, 72, 73]. Antiviral and fungicide activities were also demonstrated and linked to some flavonoids found in honey as chrysin, acacetin, and apigenin [74].
Riera et al. evaluated the Cochrane systematic reviews and protocols for complementary medicine in order to check several new therapies approved in Brazilian regulation. These studies demonstrated that honey dressings for partial healing of wound burns and honey to reduce coughing among children with acute coughs were effectively able to demonstrate scientifically and with the scrutiny of rigor science, the important safety and efficacy of honey (26 randomized clinical trials, with around 3100 individuals), supporting apitherapy introduction in the therapies proposed by the Brazilian Government [75].
Although several biological actions under Newtonian methods were already demonstrated, to think about the influence of the vibrations produced by bees when they are communicating with each other (“buzz”) and its impact on the bee products under vibrational level, the question could be: could honey be ‘carrying’ the energies of the flowers that were visited by bees during the collection of nectar and pollination? Flowers as the humans are composed of atoms, protons, electrons, and neutrons, and also, vital energy, and consequently, also possess their own frequencies. Besides it, flowers are rich in essential oils, volatile compounds that can be energized during the flights of the bees with the vibrational movements and sounds. Could honey be acting in the healthy restoration by modifying the etheric Humans Vibration? (in the level of Vital Energy,
The word propolis means ‘in defense of the city’, and it is derived from Greek
The literature already demonstrated several important biological activities for propolis as an antioxidant, anti-inflammatory, immune modulator, antibacterial, antifungal [76, 77, 78, 79, 80] and several many others [76]. Propolis was evaluated in some clinical trials with important good results in preventing respiratory tract infections in children in a randomized, double-blind, placebo-controlled, multicenter study [81], as a supplement as an adjuvant in asthmatic patients [82] and as a prophylactic for immune stimulation [83].
As it happens in the case of honey, propolis also is variable according to the region, botanical sources, climate, etc. that bees collect the exudates to transform into propolis. This was an important remarkable point for regulatory agencies such as the European Medicines Agency (EMA) to criticize the approval of propolis as a medicine, due to the lack of standardization [84]. In this sense, it was presented in 2012, a propolis standardized extract called EPP-AF® [79]. Propolis EPP-AF® was already studied according to its safety and efficacy in animal and human protocols for several applications [79, 85, 86, 87, 88, 89]. Of remarkable interest was the clinical trial done with EPP-AF® (500 mg/kg/day) in kidneys damage protocol during 12 months of research and monitoring of elderly patients, with impacting results in the reduction of proteinuria biomarkers [90]. Also, and not less impacting was the demonstration of the lack of interaction evaluated in a clinical study with hepatic enzymes CYPs and the transport protein PgP. Propolis EPP-AF® demonstrated safety at this level using WHO protocols [91]. Finally, Berretta et al. [80] published a review article supporting the use of propolis to reduce the damages and aggressiveness of SARS-CoV-2 in the COVID-19 patients. But, besides the Cartesian mechanisms already demonstrated above to propolis effects, could propolis also act by vibrational medicine? After looking forward to finding some toxicity data in animal and clinical trials for propolis, no impacting results were found, except by sparse cases of an allergic reaction [92, 93]. Some patients can be allergic to propolis due to a topical application, inhalation, or ingestion. Curiously, diluted propolis applied by inhalation reduced allergic airway inflammation system [94], besides the several studies demonstrating that propolis possesses anti-inflammatory results in several models with a huge range of dosages. In this example about allergy, Does the small dosage inhaled, could be acting following the principle of homeopathy? Could propolis be “energized” by the vibration produced by bees? This is another hypothesis to be tested if we can attribute vibrational effects to apitherapy.
But, if we consider that propolis was produced by bees after the collection of plant exudates, rich in chemical substances as flavonoids, phenolics, essential oils, etc., that possess vital energy and a vibrational standard, and suffer vibration during this process, could we postulate the hypothesis that propolis could be acting in the frequency modification on an etheric level as a vibrational medicine? Newtonian evidence already demonstrated the pharmacological effects, but could something more be happening?
Some European countries as Germany and Slovenia are using as apitherapy beehive air inhalation (“apirespiration”) to promote the health of respiratory systems. The inhaled aroma produced in beehives has been suggested to be beneficial to the health of human beings [95]. The best results usually were related to immune disorders such as allergies, chronic sinusitis, and susceptibility to infections, for patients of all ages [96].
In the beehive environment, the air is constantly circulating due to the vibration of the wings of thousands of bees. The air is saturated with water vapor, particles of isoprene, terpene, essential oils, hormones, feromones, liquid wax, alcohol, bee saliva secretions, propolis, trace elements, enzymes, choline, phytohormones, etc. By natural respiration, these substances are applied in the human body, having a positive effect on various diseases, whether as prevention, cure, or stabilization [51]. “Apirespiration” sum the benefits of the substances produced in the beehive with the vibrational energy of the flight of the bees, associated with the breathing of the pleasantly warm bee air (around 36°C). Excellent results of apirespiration have been proven with bronchitis, asthma, allergies, diphtheria, chronic inflammation, impaired immunity, migraines, and depression [51].
Several systems were proposed to this type of apitherapy, as a respiratory system connected directly to the honeycomb (Figure 3) and others completer and more planned in well-structured rooms were already suggested, the ApiHouses.
Apirespiration system.
The inhaled aroma produced in beehives has been suggested to be beneficial to the health of human beings. This integrative medicine is still an incipient field of study, and research projects in rich countries have improving its current results despite the inherent difficulties by the high number of bee species and consequently the quantitative and qualitative differences in the chemical composition of the honeybees and the concentration of their by-products (Figure 4).
Some ApiHouses proposed by [
The different models and classifications used in each case for ApiHouses were related to different kinds of experience. Some more related to the api respiration properly said, the inhalation of the volatile substances and the vibration offered by bees, but also the interaction and peace obtained with the vibration of nature and its sounds.
In the cases of ApiHouse, the question could be if the vibration/sounds produced by bees were affecting the etheric vibration of the patient modifying it to the ‘normal’ level, or if the vibration was “energizing” the volatile substances emitted by the beehive, or both.
Bee venom importance was highlighted with the publication of the First scientific paper by Desjardins, a French physician on the successful treatment and curative properties of bee venom for rheumatic disease. Filip Terc (1888) was considered as the “Father of Apitherapy”, he applied around 39,000 bee stings to over 500 rheumatic patients, was the first one to use systematically this type of therapy culminating with the publication “Report about a peculiar connection between bee stings and rheumatism” [96].
Bee venom is produced by bees for two glands (acid and alkaline glands) associated with the sting apparatus of worker bees [96]. It is a complex mixture from honey bees, which possesses various peptides including melittin, apamin, adolapin, apamin and mast cell degranulation peptide, enzymes (PLA2, hyaluronidase, acid phosphomonesterase, α-D-glucosidase, and lysophospholipase), biologically activity amines, and non-peptide components such as histamine, dopamine, and norepinephrine [97].
Bee venom therapy can be applied in some different ways, (i) live bee sting, (ii) bee venom injection, and (iii) bee venom acupuncture. The scientific data suggests that bee venom acupuncture is more effective than the application of live bee sting, bee venom injection, and acupuncture alone [96, 97]. The sum of bee venom and acupuncture offers the benefits of the chemical and pharmacological effects of the substances found in the bee venom and the mechanical application in the acupoints [97].
The number of studies already published with bee venom is too large. In order to show some application of its bee product, some interesting results will be present. Curiously, bee venom and acupuncture are very connected. Khalil et al. [98] demonstrated that bee venom acupuncture at Yanglingquan acupoint (GB34) improved locomotor behavior significantly, reduced several central amines, reduced pro-inflammatory cytokines, and neuronal apoptosis, demonstrating strong evidence of a neuroprotective effect of this therapy. Bee venom and acupuncture also demonstrated good results in idiopathic Parkinson’s disease [99]. In rheumatoid arthritis, the effects occur beginning with an anti-inflammatory action, reducing the expression of some cytokines and the level of glucocorticoids, protease activity, and reactive oxygen species (ROS) [97].
Besides the innumerous animal studies with bee venom, only some clinical trials were already performed, however, with a relatively small number of patients, for the lumbar disc disease treatments, knee osteoarthritis, rheumatoid arthritis, adhesive capsulitis, lateral epicondylitis, neuropathic pain, Parkinson disease, stroke, depression. An interesting mini-review about the use of bee venom under the point of view of safety and efficacy in acupuncture puts the question “to bee or not to be” in this topic, since the risks about the allergic reactions can be very high and so, a good evaluation of the ‘costs’ and benefits needs to be done in order to take this decision - “To bee or not to be, is that question” [100].
Now, under the point of view of vibrational therapy, definitely, the tiny quantities of the bee venom necessary to produce the effect let us know about the effect of homeopathic medicine, especially thinking about the potential toxicological effects. Would the Bees vibration offer a homeopathic characteristic to this product? The high incidence of successful results applying bee venom in the acupoints really calls attention. The substances present in bee venom, in a tiny concentration, can offer different pharmacological types of effects, including important damages. The mechanical application of needles in specific acupoints can stimulate vital energy
Other bee products could be presented here as royal jelly, bee wax, bee pollen, however, with the examples presented above it was possible to know something about apitherapy and its importance for humanity, and open the mind to think about new possibilities for the influence of bee products in living beings. If these hypotheses are true, we will only know if appropriate techniques are used. So far, it is only speculation to be tested.
It is noted that, although more comprehensive, the new concept of health adopted by the World Health Organization is still incomplete, not considering the “individual” in its entirety, which includes the vibrational and energetic aspects addressed in this chapter. Biologists have long been impressed with the ability of living things to maintain their own stability. The idea that a disease is cured by natural powers, by “
Starting from the essential unit of matter, these authors presented the biophysical links in common and specific methods for the search for homeostasis. The search for the integral man, full of its capacity for self-regulation, goes through the path of effective and progressively more natural preventive health.
Launching a vibrational look at ancient therapies (acupuncture, yoga and apitherapy), as well as for the youngest among them, homeopathy, represents contemplating and renewing the ancestral bonds that connect the three parts of the human being: body, mind and spirit. An innovative way of think in this sense were presented for homeopathy and some new hypotheses were formulated, especially regarding the function of bees as a vibrational instrument for each bee product known.
It is possible that many readers will be concerned, because the larger the body of evidence, the more it will be possible to provoke a closer look within the countless therapies presented in this book. The greater the understanding of the biophysical aspects of the energy generation process, the distribution of information, the use of nutrition for the production of defense in the body system, the more accessible and comprehensive the integrative and complementary therapies in current and future medicine will be.
The authors would like to express their gratitude to Apis Flora Indl. Coml. Ltda. for financial support to this chapter and for all efforts in demonstrating the therapeutic effects of propolis and other bee products. And also to be thankful to the QuantaVita Systemic and Oriental Therapies, Ribeirão Preto/SP, Brazil, for collaboration as a space for active observation of vibrational medicine and for providing the fundings to the lead author of this chapter (JMMG), and to CAPES (Coordination for the Improvement of Higher Education Personnel - Brazil) that provided the scholarship to the author (MCFR).
We also thank all the researchers’ morphic fields that inspired the authors with their work and hypotheses. To the masters who, since immemorial times, have contemplated nature, experienced its benefits, and experienced its assumptions. Without them, this chapter would not be possible. Thanks to the family systems we belong to, thanks to them and their evolutionary efforts, we have produced these links for an increasingly inclusive complementary medicine.
The authors declare that they have no conflict of interests.
WHO | World Health Organization |
PNPIC | National Policy of Integrative and Complementary Practices |
MS | Ministério da Saúde/Health Ministery |
HBU | Health Basics Units |
NASF | Family Health Support Units |
TCM | Traditional Chinese Medicine |
CNS | Central Nervous System |
AP | Action Potential |
SP | Spleen and Pancreas meridian |
LU | Lung meridian |
K | Kidney meridian |
L | Liver meridian |
HT | Heart meridian |
TE | Triple Burner meridian |
SI | Small Intestine meridian |
LI | Large Intestine meridian |
EMA | European Medicines Agency |
EPP-AF | Propolis Standardized Extract – Apis Flora® |
CYPs | Cytochromes |
PgP | Glicoproteína-P |
PLA2 | Phospholipase A2 |
SARS-CoV-2 | Severe acute respiratory syndrome |
COVID-19 | Coronaviruses |
Authors are listed below with their open access chapters linked via author name:
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\\n\\n\\n\\n\\n\\n\\n\\n\\n\\nJocelyn Chanussot (chapter to be published soon...)
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\\n\\nKhalil Amine 2017, 2018
\\n\\nEwan Birney 2015-18
\\n\\nFrede Blaabjerg 2015-18
\\n\\nGang Chen 2016-18
\\n\\nJunhong Chen 2017, 2018
\\n\\nZhigang Chen 2016, 2018
\\n\\nMyung-Haing Cho 2016, 2018
\\n\\nMark Connors 2015-18
\\n\\nCyrus Cooper 2017, 2018
\\n\\nLiming Dai 2015-18
\\n\\nWeihua Deng 2017, 2018
\\n\\nVincenzo Fogliano 2017, 2018
\\n\\nRon de Graaf 2014-18
\\n\\nHarald Haas 2017, 2018
\\n\\nFrancisco Herrera 2017, 2018
\\n\\nJaakko Kangasjärvi 2015-18
\\n\\nHamid Reza Karimi 2016-18
\\n\\nJunji Kido 2014-18
\\n\\nJose Luiszamorano 2015-18
\\n\\nYiqi Luo 2016-18
\\n\\nJoachim Maier 2014-18
\\n\\nAndrea Natale 2017, 2018
\\n\\nAlberto Mantovani 2014-18
\\n\\nMarjan Mernik 2017, 2018
\\n\\nSandra Orchard 2014, 2016-18
\\n\\nMohamed Oukka 2016-18
\\n\\nBiswajeet Pradhan 2016-18
\\n\\nDirk Raes 2017, 2018
\\n\\nUlrike Ravens-Sieberer 2016-18
\\n\\nYexiang Tong 2017, 2018
\\n\\nJim Van Os 2015-18
\\n\\nLong Wang 2017, 2018
\\n\\nFei Wei 2016-18
\\n\\nIoannis Xenarios 2017, 2018
\\n\\nQi Xie 2016-18
\\n\\nXin-She Yang 2017, 2018
\\n\\nYulong Yin 2015, 2017, 2018
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\n\n\n\n\n\n\n\n\n\nJocelyn Chanussot (chapter to be published soon...)
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\nYuekun Lai
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\n\nKhalil Amine 2017, 2018
\n\nEwan Birney 2015-18
\n\nFrede Blaabjerg 2015-18
\n\nGang Chen 2016-18
\n\nJunhong Chen 2017, 2018
\n\nZhigang Chen 2016, 2018
\n\nMyung-Haing Cho 2016, 2018
\n\nMark Connors 2015-18
\n\nCyrus Cooper 2017, 2018
\n\nLiming Dai 2015-18
\n\nWeihua Deng 2017, 2018
\n\nVincenzo Fogliano 2017, 2018
\n\nRon de Graaf 2014-18
\n\nHarald Haas 2017, 2018
\n\nFrancisco Herrera 2017, 2018
\n\nJaakko Kangasjärvi 2015-18
\n\nHamid Reza Karimi 2016-18
\n\nJunji Kido 2014-18
\n\nJose Luiszamorano 2015-18
\n\nYiqi Luo 2016-18
\n\nJoachim Maier 2014-18
\n\nAndrea Natale 2017, 2018
\n\nAlberto Mantovani 2014-18
\n\nMarjan Mernik 2017, 2018
\n\nSandra Orchard 2014, 2016-18
\n\nMohamed Oukka 2016-18
\n\nBiswajeet Pradhan 2016-18
\n\nDirk Raes 2017, 2018
\n\nUlrike Ravens-Sieberer 2016-18
\n\nYexiang Tong 2017, 2018
\n\nJim Van Os 2015-18
\n\nLong Wang 2017, 2018
\n\nFei Wei 2016-18
\n\nIoannis Xenarios 2017, 2018
\n\nQi Xie 2016-18
\n\nXin-She Yang 2017, 2018
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