Glyphosate [N-(phosphonomethyl) glycine] (GPS) is currently the most commonly applied herbicide worldwide. Given the widespread use of glyphosate, the investigation of the relationship between glyphosate and soil ecosystem is critical and has great significance for its valid application and environmental safety evaluation. However, although the occurrence of glyphosate residues in surface and groundwater is rather well documented, only few information are available for soils and even fewer for air. Due to this, the importance of developing methods that are effective and fast to determine and quantify glyphosate and its major degradation product, aminomethylphosphonic acid (AMPA), is emphasized. Based on its structure, the determination of this pesticide using a simple analytical method remains a challenge, a fact known as the “glyphosate paradox.” In this chapter a critical review of the existing literature and data comparison studies regarding the occurrence and the development of analytical methods for the determination of pesticide glyphosate in soil and air is performed.