Theoretical densities of the most recurring polymers found in the environment.
\r\n\tSQL is worth learning because it’s a programming language that’s in demand in the tech industry and in other sectors that need technology. Most software developers who know SQL earn respectable salaries. Learning SQL can not only enhance your skills, but it can also give you a better understanding of the applications you work with daily. In this book, we will go through the details of SQL and how to use it effectively. The goal of this book is to have many practical application examples that will help learners easily acquire and self-study SQL.
",isbn:"978-1-83969-946-7",printIsbn:"978-1-83969-945-0",pdfIsbn:"978-1-83969-947-4",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"d1d908cd61561c1e813552fbd6cb9ed1",bookSignature:"Ph.D. Duc-Man Nguyen and Dr. Van-Loi Nguyen",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11919.jpg",keywords:"Database Classification, Types of Databases, SQL Basic, Drop Statement, Aggregate Functions, Conversion Function, Date Function, Mathematical Functions, User-Defined Types, User-Defined Functions, String Data Type, Pivoting Data in SQL",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 3rd 2022",dateEndSecondStepPublish:"July 6th 2022",dateEndThirdStepPublish:"September 4th 2022",dateEndFourthStepPublish:"November 23rd 2022",dateEndFifthStepPublish:"January 22nd 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"a month",secondStepPassed:!0,areRegistrationsClosed:!1,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Dr. Nguyen Duc Man is a member of the DSA-Da Nang Software Association, Vietnam. He was awarded the Award for Excellent effort in Training and Management by the Duy Tan University, Vietnam for several years in a row, and received the Certificate of Merit for Excellent effort in Training and Management by the Ministry of Education and Training, Vietnam. His current research interests are software testing, mobile testing, test automation, test case generation, context-driven testing, and ML for testing.",coeditorOneBiosketch:"Dr. Van-Loi Nguyen received his Master of Engineering in Computer Science from the University of Danang, Vietnam in 2010, and a Ph.D. degree from Soongsil University, Korea, in 2017. He is currently a lecturer at the Vietnam - Korea University of Information and Communication Technology, the University of Danang. He has over 18 years of experience teaching and researching programming, databases, machine learning, information retrieval, multimedia, and artificial intelligence.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"227628",title:"Ph.D.",name:"Duc-Man",middleName:null,surname:"Nguyen",slug:"duc-man-nguyen",fullName:"Duc-Man Nguyen",profilePictureURL:"https://mts.intechopen.com/storage/users/227628/images/system/227628.jpg",biography:'1.\tName:\tNguyen Duc Man\n\tOffice: Room 601, 254 Nguyen Van Linh, Danang, Vietnam\n\tTel: +84-2363 650 403 (Ext 601)\n Mobi: 0904 235 945\n\tEmail: mannd@duytan.edu.vn\n\n2.\tEducation:\nBSc.\tInformation Technology\tDuy Tan University, Vietnam\t1999\nMSc.\tComputer Science\tDanang University, Vietnam\t2009\nPhD.\tComputer Science\tDuy Tan University, Vietnam\t2020\n\n3.\tAcademic experience:\nDuy Tan University, Vietnam\tTeaching\tLecturer\t2004- Present\tFT\n\t\n4.\tNon-academic experience:\nHSD Corporation, Ho Chi Minh, Vietnam\tAnalysis, Design and Code, DB Design\tSoftware Developer\t1999-2001\tFT\nDuy Tan Software Center, Vietnam\tTeam leader, Planning, A&D\tProject Lead\t2001-2003\tFT\n\n5.\tCertifications or professional registrations:\n-\tCertificate for completion of Train the Trainer courses (Software Capstone Project, Requirements Engineering, Software Architecture, Software Project management, software Process and Quality management, Software Integration Practices). Institute of Software Research, Carnegie Mellon University, USA. (2010, 2014, 2016, 2017, 2018, 2019).\n-\t7 Professional Development Hours for participation in the Fundamentals of Program Assessment Workshop, ABET Symposium (2017)\n-\t7 Professional Development Hours for participation in the Self-Study Development Workshop ABET Symposium (2017)\n-\t14 Professional Development Hours for participation at the 2017 ABET\nSymposium, ABET Symposium (2017)\n-\tSoftware Testing and Automation Conference. VISTACON 2011, Ho Chi Minh, Vietnam (2011).\n-\tFagan Software Inspection Method. ECCI Group, Vietnam (2011).\n-\tHP Train the Trainer courses: HP QTP, LoadRunner, Quality Center (2011).\n\n6.\tMembership in professional organizations:\n-\tMember of DSA-Da Nang Software Association, Vietnam.\n\n7.\tHonors and awards:\n-\tAwards for Excellent effort in Training and Management. Duy Tan University, Vietnam (2003, 2006, 2007, 2008, 2009, 2010, 2012).\n-\tCertificate of Merit for Excellent effort in Training and Management. Ministry of Education and Training, Vietnam (2008- 2009, 2010- 2011, 2013- 2014).\n-\tCertificate of Merit of the People Committee of Da Nang. Danang, Vietnam (2011-2012).\n\n8.\tService activities:\n-\tInstitutional service: \n•\tStudents’ Awards Committee \n•\tFaculty Development Committee \n•\tScholarship Committee \n•\tFaculty Council \n\n9.\tPublications and presentations from the past five years:\n\n1.\t\tCheng, Y. H., Chang, P. C., Nguyen, D. M., & Kuo, C. N. (2020). Automatic Music Genre Classification Based on CRNN. Engineering Letters, 29(1).\n2.\t\tHuynh, Q. T., Pham, L. T., Ha, N. H., & Nguyen, D. M. (2020). An Effective Approach for Context Driven Testing in Practice—A Case Study. International Journal of Software Engineering and Knowledge Engineering, 30(09), 1245-1262.\n3.\t\tNguyen, D. M., Huynh, Q. T., Ha, N. H., & Nguyen, T. H. (2020). Automated test input generation via model inference based on user story and acceptance criteria for mobile application development. International Journal of Software Engineering and Knowledge Engineering, 30(03), 399-425.\n4.\t \tNguyen, D. M., Do, H. N., Huynh, Q. T., Vo, D. T., & Ha, N. H. (2018, August). Shinobi: A Novel Approach for Context-Driven Testing (CDT) Using Heuristics and Machine Learning for Web Applications. In International Conference on Industrial Networks and Intelligent Systems (pp. 86-102). Springer, Cham.\n5.\t\tHoang-Nhat, D. O., NGUYEN, D. M., HUYNH, Q. T., & Nhu-Hang, H. A. (2018). One2Explore-Graph Builder for Exploratory Testing from a Novel Approach.\n6.\t\tNguyen, M. D., Huynh, T. Q., & Nguyen, T. H. (2016, November). Improve the Performance of Mobile Applications Based on Code Optimization Techniques Using PMD and Android Lint. In International Symposium on Integrated Uncertainty in Knowledge Modelling and Decision Making (pp. 343-356). Springer, Cham.\n7.\t\tBao Le Nguyen, Nguyen Duc Man, Minh Nguyen Cong and Luong Vo Van (2013). Difficulties in the Operation of an International Program in Vietnam. FICAP-1 Proceedings, BrownWalker Press, 2013, ISBN-13: 9781612337043.\n8.\t\tDuc Nguyen Duc Man, Tien Vu Truong, Nguyen Bao Le (2013). Deployment of Capstone Projects in Software Engineering Education at Duy Tan University as Part of a University-wide Project-based Learning Effort. Learning and Teaching in Computing and Engineering (LaTiCE), IEEE Computer Society -CPS, 2013, E-ISBN :978-0-7695-4960-6 (pp. 184 -191).\n9.\t\tGia Nhu Nguyen, Nhat Tan Tran, Thanh Trung Nguyen and Nguyen Duc Man (2014). The Benefits of CDIO for ABET Preparation from a Hands-on Study in Vietnam. Proceedings of the 10th International CDIO Conference. Barcelona \n10.\t\tVu T Truong, Bao N Le, Man N Duc, Thang M Nguyen (2014). Accessing the Maturity of Teamwork Capabilities through CDIO Projects. Proceedings of the 10th Annual International CDIO Conference. Universitat Politècnica de Catalunya, Barcelona, Spain.\n11.\t\tPhuong A Pham, Man D Nguyen, Long Q Nguyen, Thang M Nguyen, Bao N Le (2014). Learning Computer Programming In Cdio’s Team Settings. Proceedings of the 10th Annual International CDIO Conference. Universitat Politècnica de Catalunya, Barcelona, Spain.\n12.\t\tVo, Q. N., Tran, N. P., Van Dat Ngo, V. H. T., Huynh, Q. T., Ha, N. H., & Nguyen, D. M. LEVERAGE THE BLOCKCHAIN TECHNOLOGY TO MANAGE SMART CONTRACT IN ASSET TRADING. Kỷ yếu Hội nghị KHCN Quốc gia lần thứ XII về Nghiên cứu cơ bản và ứng dụng Công nghệ thông tin (FAIR); Huế, ngày 07-08/6/2019 DOI: 10.15625/vap.2019.00032\n\n13.\t\tHa, N. H., Nguyen, D. M., Liu, C. A., Van Van, T., Nguyen, A. D., & Huynh, Q. T. AN EMPIRICAL STUDY OF THE IMPACT OF THE MPOS SYSTEM ON THE PROCESS CHANGE OF RESTAURANTS. Kỷ yếu Hội nghị KHCN Quốc gia lần thứ XII về Nghiên cứu cơ bản và ứng dụng Công nghệ thông tin (FAIR); Huế, ngày 07-08/6/2019 DOI: 10.15625/vap.2019.00032\n\n14.\t\tNguyễn Thanh Hùng, Nguyễn Đức Mận, Huỳnh Quyết Thắng (2019), Thử Nghiệm Đánh Giá Áp Dụng Một Số Kỹ Thuật Kiểm Thử Để Nâng Cao Độ Tin Cậy Cho Ứng Dụng Di Động Trong Môi Trường Phát Triển Linh Hoạt. Section on Information and Communication Technology (ICT) - No. 13, Journal of Science and Technique - Le Quy Don Technical University - No. 199, ISSN 1859-0209\n15.\t\tHuỳnh Quyết Thắng, Nguyễn Đức Mận, Nguyễn Thị Bảo Trang, Nguyễn Thị Anh Đào (2016). Kỹ thuật kiểm thử hồi qui hiệu quả cho phát triển ứng dụng di động. Kỷ yếu Hội nghị khoa học công nghệ quốc gia lần thứ IX, ngày 4-5/8/2016 - "Nghiên cứu cơ bản và ứng dụng Công nghệ thông tin" (FAIR 2016), trang 255-265. Nhà xuất bản Khoa học tự nhiên và Công nghệ. ISBN 978-604-913-472-2\n\n10.\tRecent professional development activities:\n-\tCoordinator and Assistant Director of ACM/ICPC Asia Regional Contest, Danang, Vietnam (2013). \n-\tParticipated in the 7th National Conference on Fundamental and Applied IT Research (2014).\n-\tAttended the 7 Professional Development Hours the Fundamentals of Program Assessment Workshop (2017).\n-\tParticipated in the 12th National Conference on Fundamental and Applied IT Research (2019).\n-\tINISCOM 2018, INISCOM 2019, KSE 2019, CITA2021, CITA2022\n-\tAttended the CDIO Regional Meeting - Asia-Pacific (2019).',institutionString:"Duy Tan University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Duy Tan University",institutionURL:null,country:{name:"Vietnam"}}}],coeditorOne:{id:"473326",title:"Dr.",name:"Van-Loi",middleName:null,surname:"Nguyen",slug:"van-loi-nguyen",fullName:"Van-Loi Nguyen",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003Sa5QKQAZ/Profile_Picture_2022-05-10T10:01:50.jpg",biography:"Dr. Van-Loi Nguyen received his Master of Engineering in Computer Science from the University of Danang, Vietnam in 2010, and a Ph.D. degree from Soongsil University, Korea in 2017. He is currently a lecturer at the Vietnam - Korea University of Information and Communication Technology, the University of Danang. He has over 18 years of experience teaching and researching in the fields of programming, databases, machine learning, information retrieval, multimedia, and artificial intelligence.",institutionString:"University of Danang",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"9",title:"Computer and Information Science",slug:"computer-and-information-science"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"429342",firstName:"Zrinka",lastName:"Tomicic",middleName:null,title:"Ms.",imageUrl:"https://mts.intechopen.com/storage/users/429342/images/20008_n.jpg",email:"zrinka@intechopen.com",biography:"As an Author Service Manager, my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. Whether that be identifying an exceptional author and proposing an editorship collaboration, or contacting researchers who would like the opportunity to work with IntechOpen, I establish and help manage author and editor acquisition and contact."}},relatedBooks:[{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. 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For instance, a typical mixing problem deals with the amount of salt in a mixing tank. Salt and water enter to the tank at a certain rate, they are mixed with what is already in the tank, and the mixture leaves at a certain rate. This process is modeled by an ordinary differential equation (ODE), as Groestch affirms: “The direct problem for one-compartment mixing models is treated in almost all elementary differential equations texts” [2].
\nInstead of only one tank, there is a group, as it was stated by Groestch: “The multicompartment model is more challenging and requires the use of techniques of linear algebra” [2]. In particular, the ODE system-associated matrix deserves to be studied since it determines the qualitative behavior of the solutions.
\nIn several previous papers and book chapters [3, 4, 5, 6], MPs were studied from different points of view. In the first paper [3], a particular MP with three compartments was proposed, and after applying Laplace transform, this example was connected with important concepts in reactor design, like the transference function. 2 years later, another work [4] analyzed more general MPs in order to obtain characterization results independent of the internal geometry of the tank system. In the third paper [5], the educative potential of MPs was studied, focusing on inverse modeling problems. Finally, in a recent book chapter [6], results for MPs with and without recirculation of fluids were analyzed, and other general results were obtained.
\nIn all these works, a given MP is modeled through an ODE linear system, in which qualitative properties (like stability and asymptotic stability) depend on the eigenvalues and eigenvectors of the associated matrices, so-called MP-matrix.
\nTaking into account previous results about MP-matrices, and the new ones presented here, two main conjectures can be proposed:
All the solutions of a given MP are stable.
If the MP corresponds to an open system, then the solutions are asymptotically stable.
In order to investigate if these conjectures—among others, introduced in the following sections—are true or not, MP-matrices (i.e., square matrices associated to the ODE linear system that models a given MP) should be deeply analyzed.
\nIn this section we introduce a specific terminology useful to allow understanding of the terms properly.
\nIn order to analyze MPs and MP-matrices, we begin by studying a problem already considered in a previous book chapter [6], which involves a tank with five compartments, shown in Figure 1.
\nA tank with five internal compartments.
In this scheme,
For instance, if \n
The ODEs associated with the central compartments (
Finally, for the right (fifth) container, we have:
\nIf all these equations are put together, the following ODE system is obtained:
\nAfter some algebraic manipulations, the corresponding mathematical model can be written as
The system-associated matrix (MP-matrix) is
\nHereafter, we will call MP-matrix to any ODE system-associated matrix related to a given MP, like matrix \n
In the previous example, the MP-matrix obviously depends on the numbers given to the different containers. In that example it was possible to enumerate the compartments such that the flux always goes from the ith compartment to the jth one, where
In general, if in a given MP it is possible to enumerate the containers such that the flux always goes from the ith compartment to the jth one, with
Now, let us analyze a different problem, where a couple of tanks are linked by all possible connections between them, including recirculation from the second tank back to the first one, as in Figure 2. This problem represents an interesting variation of an MP analyzed by Zill [7] in his textbook, where the main difference is that this new MP has no incoming and/or outgoing flux, i.e., it is a closed system.
\nTwo tanks with recirculation and no incoming or outgoing fluxes.
If in a given MP we have that \n
Taking into account the abovementioned nomenclature, the example considered in Figure 2 corresponds to an MP-CS, while the MP analyzed in Zill’s textbook [7] is an MP-OS, and both are systems with recirculation.
\nFinally, it is important to observe that in both examples (Figures 1 and 2), we have \n
In that case all the compartment volumes remain constant, and so if in an MP the following equation \n
Taking into account all these terms, several previous results can be reformulated, as shown in the next section.
\nIn order to give some general results, it is convenient to consider two different situations: MP without recirculation and MP with recirculation.
\nConsidering again the example in Figure 1, it is possible to enumerate the compartments, such that the flux always goes from the ith container to the jth one, being \n
Analyzing the system (Eq. (4)), it is easy to observe that for the jth container, the ODE right hand side is a linear combination of a subset of
being
As a consequence, under the previous conditions, the corresponding ODE system has an associated upper matrix.
\nRevisiting the ODE system (Eq. (4)), corresponding to Figure 1, it can be rewritten as
\nIt follows that for the jth compartment, the coefficient corresponding to
However, not all of these results can be extended to MPs with recirculation as will be analyzed in the following subsection.
\nIn previous works [4, 5], a “black box” system was analyzed (see Figure 3), in order to obtain a necessary condition to be satisfied by any MP-matrix with any number of compartments and unknown internal geometry. In Figure 3
A “black box” tank system.
If all volumes
Then, Eq. (9) is obtained without any consideration of the internal geometry of the tank system and can be easily verified in the previous example (see Figure 1). In fact, by adding the equations of the ODE system (Eq. (4)), it follows straightforward that the condition given in Eq. (9) is satisfied. The same conclusion can be drawn from other possible examples, corresponding to open or closed MPs, with or without recirculation. For instance, in the case schematized in Figure 2, the ODE system can be written as follows:
\nOperating with these equations, it can be proved that \n
The previous result can be generalized as follows: in a given MP—with or without recirculation—with input and output concentrations
An analogous condition may be used to know if a given matrix may or may not be an MP-matrix. For this purpose, let us consider the MP-matrix
It is easy to observe that
\nThis equation can be written as \n
If there exists an incoming (and outgoing) flux
It can be noted that Eq. (12) and Eq. (13) are particular cases of the following result: in a given MP—with or without recirculation—with an incoming and outgoing flux
Then, independently of the internal geometry of the system, the following condition is satisfied:
\nNow, let us consider again the MP-matrix
If
It is easy to observe that this new matrix will not satisfy the condition given by Eq. (14). Moreover, there is no MP associated to this matrix
As a first consequence, not every square matrix is an MP-matrix. A second observation is that if a given MP-matrix is slightly changed, the result is not necessarily a new MP-matrix.
\nFurthermore, if volumes
After interpreting the previous results, we note that when working with MP-matrices, existence, uniqueness, and stability questions for the inverse-modeling problem have negative answers.
\nThe same situation can be observed in many other inverse problems [2], and it is not an exclusive property of compartment analysis.
\nWe start this section explaining three simple and intuitive terms.
\nFirstly, we will consider that an input tank is a tank with one or more incoming fluxes. Secondly, a tank with one or more outgoing fluxes will be called output tank. Finally, we will say that an internal tank is a tank without incoming and/or outgoing fluxes to or from outside the system.
\nTaking into account the previous nomenclature, if \n
Input and output tanks are not mutually exclusive. For instance, in Figure 4, the first tank is an input tank, and at same time, it is an output tank, since it has an incoming flux \n
A tank system with recirculation and with incoming and outgoing fluxes.
Another interesting example was proposed by Boelkins et al. [8]. The authors considered a three-tank system connected such that each tank contains an independent inflow that drops salt solution to it, each individual tank has a separated outflow, and each one is connected to the rest of them with inflow and outflow pipes. In this case, all tanks are input and output ones, and there is no internal tank.
\nIt is important to mention that those types of tanks or compartments play different roles in the ODE-associated system and also—as a consequence—in the corresponding MP-matrix. In order to show this fact, let us examine a three-tank system with all the possible connections among them, as in Figure 5.
\nThree tanks with all the possible connections.
As a first remark, Figure 5 system has recirculation—unless \n
In the mass balance for the first tank—which is an input one—a term \n
Once again, the ODE system can be written as
In the previous ODE system, the independent vector is:
\nIt is easy to observe that the outgoing flux \n
The Gershgorin circle theorem first version was published by S. A. Gershgorin in 1931 [9]. This theorem may be used to bind the spectrum of a complex
If
This theorem was widely used in previous book chapters [6, 10, 11] in order to obtain new results about matrices corresponding to chemical problems.
\nHere, the main purpose is to apply this theorem to MP-matrices as a method to bind their eigenvalues, depending on the characteristics of the MP ODE system, and, even more, the compartment considered.
\nFor instance, if we consider the MP corresponding to Figure 5, the first ODE of Eq. (17) can be expressed as \n
This equation—which obviously corresponds to an input tank—gives the first row of the MP-matrix (Eq. (18)) that can be written as \n
The Gershgorin disk corresponding to this row is centered at \n
Now, if a flux balance is performed in this input tank, we have this equation: \n
The Gershgorin disk corresponding to an input tank.
Now, if the second ODE of Eq. (17) is considered, this equation can be written as \n
This internal tank equation corresponds to the second row of the MP-matrix (Eq. (18)): \n
The Gershgorin disk corresponding to this row is centered at \n
Now, if a flux balance is performed in this internal tank, we have this equation: \n
The Gershgorin disk corresponding to an internal tank.
Finally, if the third ODE of Eq. (17) is considered, this equation can be written as \n
This output tank equation corresponds to the third row of the MP-matrix Eq. (18): \n
The Gershgorin disk corresponding to this row is centered at the point \n
The flux balance in this case gives \n
Taking into account all these results, the Gershgorin circles for the MP of Figure 5 are shown in Figure 8.
\nGershgorin circles for a three-tank system with recirculation.
Since every eigenvalue lies within at least one of the Gershgorin disks, it follows that
In the following section, these results—among others—will be generalized.
\nAs stated in Section 3, if there is no recirculation, then the ODE system has only negative eigenvalues of the form
In a previous work [6], it was proved that in an open MP, with three or less compartments, with or without recirculation, all the corresponding ODE system solutions are asymptotically stable.
\nIt is important to analyze if this result can be generalized or not, when closed systems and/or tanks with more than three compartments are considered. For this purpose, we will start with the following theorem.
\nIn an open system, if the ith tank is an input one, then the diagonal entry of the ith row is \n
If \n
This equation gives.
\nEq. (20) implies that the ith row of the MP-matrix has entries: \n
A flux balance gives \n
In an open system, being the ith tank an input one, the Gershgorin circle corresponding to the ith row looks like the disk in Figure 6.
\nIf in an open system, all are input tanks, all the eigenvalues satisfy the condition \n
In an open system, if the ith tank is not an input one, then the diagonal entry of the ith row is \n
If \n
This equation gives:
\nEq. (22) implies that the ith row of the MP-matrix has entries \n
In this case a flux balance gives the following equation \n
In an open system, if the ith tank is not an input one, the Gershgorin circle corresponding to the ith row looks like the disk in Figure 7.
\nIn an open system, the Gershgorin disks look like those of Figure 8.
\nAs a consequence of the previous results, the following corollary can be stated.
\nIn an open system with input and non-input tanks, all the eigenvalues satisfy the condition \n
Independently of the previous results, it is easy to observe that all the solutions corresponding to the eigenvalues with \n
For this purpose, when analyzing eigenvalues with \n
In the first case, the corresponding ODE solutions are a linear combination of the functions \n
In the second case—which really happens, as it will be observed later—we have \n
According to the position of the Gershgorin disks for an MP-matrix (see Figure 8), the ODE solutions corresponding to an eigenvalue \n
For this purpose it is important to observe that if an eigenvalue \n
In this case the ODE solutions are a linear combination of the following functions: \n
Considering all these results, it is obvious that the stability of the ODE system solutions will depend exclusively on \n
In the previous section, some particular cases with \n
Three tanks with all the possible connections.
Other questions are not so simple like the previous one. The next two examples propose challenging problems that deserve to be studied:
\nQuestion 1:
\nIs it possible to find an MP-matrix with an eigenvalue \n
Question 2:
\nIs it possible to find an MP-matrix such that \n
Question 3:
\nIs it possible to find an MP-matrix with complex eigenvalues in an open system?
\nFinally, it is interesting to observe that all cases analyzed here with \n
Mixing problems are interesting sources for applied research in mathematical modeling, ODE, and linear algebra, and—as it was shown—their behavior depends on how they are connected. It has been proved that null eigenvalues are not expected in open systems with three or less components, and
As a final remark, all the MP differential equation systems considered in this chapter have stable or asymptotically stable solutions. Nevertheless, this situation may change depending on the answers to the questions and the conjecture presented in the last section, giving a challenging proposal for further research on this topic.
\nThe author wishes to thank Marjorie Chaves for her assistance and support in this work.
\nWe live in the “Plastic Age”. From its creation in the early 1870, plastic material has largely contributed to the society development making everyday life easier. Plastic material offer good advantages as it can be customized with specific shapes and chemical and physical properties i.e., elasticity, hardness, lightness, transparency and durability. Due to this, the production has dramatically boosted annual plastic production from 0.5 million tons in the 40s to 550 million tons in 2018 [1]. However, plastics sturdiness presents some negative implications as the increasing rate of plastic consumption worldwide its release in the environment associated with a low degradation rate is resulting in its accumulation in coastal and marine sediments, pelagic and benthic biota from coastal to open ocean areas at each latitude from the poles to the equator. Depending on sources and formation mechanisms plastic fragments are split into “primary” and “secondary”. Primary plastics are resulting from the direct input of freshly manmade emissions, adding new micronized size by-design plastic material to the environment. According to this definition, major sources primary plastics are: (A) polymers intentionally produced and used as such. In this group belong i.e., personal care consumer products, industrial or commercial products and other specialty chemicals with plastic microbeads; (B) inherent collateral products of other industrial activities or (C) plastic sourced as accidental or deliberate spillage i.e., pellets loss from plastic factories and transport. In contrast, secondary plastics are associated as secondary pollution sources where larger plastic items undergo degradation and subsequent fragmentation leads to the formation of smaller plastic pieces as they start to break down by photo-oxidative degradation followed by thermal and/or chemical degradation [2].
\nWhile addressing the comprehension of plastics degradation mechanisms in marine aquatic environments it is useful to divide them into plastics with a carbon-carbon backbone and plastics with heteroatoms in the main chain. Some of the most environmentally recurrent polymers like polyethylene, polypropylene, polystyrene and polyvinylchloride have a pure carbon-based backbone. On the contrary, polyethylene terephthalate and polyurethane plastics have heteroatoms in the main chain. Most packaging materials are made of plastics with a carbon-carbon backbone structure. As they are very often discarded after a short period of time, there is a high potential to observe significant loading in the environment. All these polymers are susceptible to photo-initiated oxidative degradation, which is believed to be their most important abiotic degradation pathway in aerobic outdoor environments. This degradation pathway consists of a complex sequential multi-step process where initially chemical bonds in the main polymer chain are broken down by light, by heat or by a combination of both to produce a free radical formation [3, 4]. Polymer radicals react with oxygen and form a peroxy-radical species. As a side effect, the co-occurring formation of hydroperoxides promotes a further complex pathway of radical reactions leading to significant autoxidation of the target polymer. These processes ultimately lead to chain scission, branching and creation of oxygen-containing functional groups. As the molecular weight of the polymers is reduced, the material becomes fragile and is more vulnerable to fragmentation, which makes a higher surface area reactive to further degradation. Nevertheless, anti-oxidants and stabilizers used as additives inhibit the degradation of the polymer. Thus, degradation rates depend strongly on used additives and plasticizers [4]. In most cases these are well-known toxic chemicals not covalently bonded to the polymer and therefore capable of leaching out from the plastic during the degradation process, and easily enters into the aquatic environment representing a further point of concern for eco-toxicologists. On the other hand, different degradation mechanisms cause degradation of plastics with heteroatoms in the main chain. They show an increased thermal stability compared to polymers with a simple carbon backbone. Under marine environmental conditions the degradation processes of plastics like polyethylene terephthalate (PET) or polyurethane (PU) are normally controlled by hydrolytic cleavage. Similar to carbon-carbon backbone plastic polymers, PET can undergo photo-induced autoxidation via radical reactions leading to the ultimate formation of a carboxylic acid end groups, which show a promoting effect on thermo- as well as photo-oxidative degradation. Weathering of PET in the marine environment occurs mainly by photo-induced oxidation and secondly by hydrolytic degradation processes which cause the yellowing of the polymer. For thermo-oxidative degradation the consequences are an in the content of the some end groups i.e., carboxylic acid as well as a general decrease in molecular weight of the main polymer [4]. Hydrolysis also leads to a reduction in molecular weight and an increase in carboxylic acid end groups. PET is highly resistant to environmental biodegradation because of its compact structure [4]. On the other hand, polyurethane-like compounds show carbon, oxygen and nitrogen in the main chain demonstrating enhanced susceptibility to degradation via photo-oxidation, hydrolysis and biodegradation. Plastic floating on the ocean surface is exposed to moderate temperatures, solar radiation at wavelengths of 300 nm and longer, as well as oxidizing conditions. Since temperatures are moderate, the most important factors initiating abiotic degradation are oxygen and sunlight. According to recent studies, fragmentation patterns first occur at the plastic surface, which is exposed and available for chemical or photo-chemical attack. The process is more efficient with smaller plastic fragments as they show a higher surface to volume ratio [5]. Changes in color and crazing of the surface are the initial visual effects of polymer degradation. Surface cracking makes the inside of the plastic material available for further degradation, which eventually leads to embrittlement and disintegration. Furthermore, almost all commercial plastics include additives. These co-production chemicals embedded in the polymers can also leach into the aquatic environment, which is an additional point of concern. As these substances enhance plastics’ resistance to degradation, it becomes difficult to quantitatively estimate the fragmentation patterns since different plastic products can vary in their composition. On the other hand, additional factors can significantly influence degradation rates as floating plastic may develop biofilms that shield it from UV radiation. The formation of biofilm in plastic microliter collected from the marine aquatic environment has been previously documented worldwide [6, 7, 8]. Such phenomena could lead to a reduction in photo-initiated degradation. So far, there have been very few studies of degradation mechanisms for plastic polymers in the marine environment although some promising early findings have been reported by ongoing joint research initiatives (e.g., JPI-Weather Mic and JPI-PlasTox). The biofilm formation can also affect the vertical distribution of plastic fragments largely affecting their distribution in the water column or in the sedimentary environment. Most synthetic polymers are buoyant in water and substantial quantities of plastic debris that are buoyant enough to float in seawater are transported and potentially washed ashore. The polymers that are denser than seawater tend to settle near the point where they entered the environment; however, they can still be transported by underlying currents. Table 1 resumes the theoretical densities of the most recurring polymers found in the environment. Microbial films rapidly develop on submerged plastics and change their physicochemical properties such as surface hydrophobicity and buoyancy [9, 10]. All in all, plastic debris is a mixture of molecules and chemicals, its size ranging from some meters to a few micrometers and probably nanometers. It is derived from a broad variety of origins, such as fishing gear, nets, bottles, bags, food packaging, taps, straws, cigarette butts and cosmetic microbeads and the associated fragmentation of all of these. Plastic debris has become ubiquitous in all environmental compartments of the marine ecosystem form sediments to sea surface. Thus, the observed loadings floating in the ocean represents only a limited portion of the total input. It has been previously reported that most plastic litter ends up on the seabed with a remaining fraction distributed on beaches or floating on the seawater surface leading one to consider that merely quantifying floating plastic debris may lead to a significant underestimation of the actual amount of plastics in aquatic environments [11].
\nPolymer | \nAbbreviation | \nDensity (g/cm3) | \nApplications | \n
---|---|---|---|
Expanded polystyrene | \nEPS | \n0.01–0.04 | \nBait boxes, floats, cups | \n
Low density polyethylene | \nLDPE | \n0.89–0.93 | \nPlastic bags, bottles, gear, cages | \n
High density polyethylene | \nHDPE | \n0.94–0.98 | \nPlastic bags, bottles, gear, cages | \n
Polypropylene | \nPP | \n0.83–0.02 | \nRope, bottle caps, | \n
Polypropylene terephthalate | \nPET | \n0.96–1.45 | \nBottles, gear | \n
Styrene butadiene rubber | \nSBR | \n0.94 | \nCar tyre | \n
Polyamide | \nPA | \n1.02–1.16 | \nGera, fish farm nets, rope | \n
Polystyrene | \nPS | \n1.04–1.10 | \nContainers, packaging | \n
Polymethyl methacrylate | \nPMMA | \n1.09–1.20 | \nInsulation, packaging | \n
Polyvinylchloride | \nPVC | \n1.16–1.58 | \nFilm, pipe, containers | \n
Polycarbonate | \nPC | \n1.20–1.22 | \nTextiles, leisure boats | \n
Polyurethane | \nPU | \n1.20 | \nInsulation, floats | \n
Alkyd | \nALK | \n1.24–2.10 | \nPaints, packaging | \n
Polyester | \nPES | \n1.24–2.3 | \nTextiles, | \n
Polytetrafluoroethylene | \nPTFE | \n2.1–2.3 | \nPersonal care products | \n
Theoretical densities of the most recurring polymers found in the environment.
Overall ecosystem health can be significantly affected by the accumulation of trash and plastics in our seas. Ingestion of and entanglement in marine debris directly impacts marine life. Laboratory studies provide a strong proof of evidence for the effects of microplastic ingestion observed in organisms collected from the natural environment. Indeed, in laboratories, under natural like conditions, microplastics have been shown to be ingested by amphipods, barnacles, lugworms and bivalves [12, 13, 14]. In the same organisms, the uptake of microplastics caused notable ultrastructural changes in the investigated tissues including histological changes as well as cell functioning impairments [15]. In field observations, the occurrence of MPs in the gastrointestinal tract and gills of pelagic and demersal fish and marine mammals has been documented [16, 17]. Past reports have shown that many marine organisms wrongly identify plastic debris for food. Ingestion of marine debris induce different deleterious effects such as pathological alteration, starvation and mechanical blockages of digestive processes. Furthermore, the interaction of plastic fragments, especially those at micrometric and nanometric scales, with organic pollutants are of importance in relation to environmental contamination and biological effects on organisms in the water column as well as in the sedimentary environment [18, 19]. Hydrophobic pollutants co-occurring in the aquatic environment may in fact adsorb onto MP debris. According to the different sizes, plastic fragments have the potential to transport contaminants more effectively through biological membranes and ultimately inside cells of aquatic organisms. The presence of organic pollutants on marine plastics has been illustrated for a wide range of chemicals in natural aquatic conditions [20, 21]. The exposure routes of organic pollutant-enriched MPs are varied, while the toxicity is largely inversely correlated to the size of the particles, as the smaller the particle the further into the organism it can penetrate releasing toxic chemicals under acidic gut conditions [22]. According to the properties of the adsorbed chemicals, several toxicity mechanisms are represented by increased oxidative stress, genotoxicity, depletion of immune competence, impairment of key cell functioning, loss in reproductive performance, disorders in energy metabolism, and changes in liver physiology [23, 24, 25].
\nDifferent methods have been developed for identifying plastics, including meso, micro and nanoplastics in water, sediments and biota as well as to a lesser extent in soil. The percentage of organic matter (OM) in general as well as some recurring specific macromolecules, such as fats and proteins may hamper the analysis, thus hiding plastic fragments in visual analyses and distort signals in Fourier transformed infrared (FT-IR) and Raman spectroscopy, two of the most frequently used methods for plastic identification [26, 27]. Hence, identifying and quantifying plastic materials in organic matter enriched samples may be a challenge. In sediments, several available protocols recommend a preliminary sorting of plastic size grounding and sieving. After sieving, the mineral phase of soils might be removed easily using density fractionation methods. Different density solutions have been used including NaCl, ZnCl2, NaI and more recently 3Na2WO4 9WO3 H2O to obtain dense floating solutions [28, 29]. However, it has been shown that simple density fractionations will not succeed in separating organic matter from plastic materials in sediments because most of the OM show densities between 1.0 and 1.4 g/cm3, similar to that of several environmentally recurring plastic types like PET, PP, PE and Nylon. Sufficient removal of OM without destroying small plastic polymers is challenging because large parts of OM are refractory. At the same time, polymers show strong sensitivity to acidic or strong oxidizing treatment conditions, which induce permanent modifications (e.g. yellowing), thus hampering their classification by microscope-oriented techniques. To efficiently remove OM, multistep extraction, purification processes based on alkaline treatments possibly combined with multi-enzymatic digestion steps have been suggested for the analyses of biota water or sediments. Enzymatic digestion has been promising for the removal of organic as well as other interferents, such as chitin, agar and lipid enriched samples [27]. Strong alkali digestions have been pointed out as being effective for sediments as well as biological samples, without altering the plastic itself [30]. While on the contrary and as previously mentioned, strong acidic conditions induce partial dissolution of polycarbonate as well as partial digestion of polyethylene and polypropylene [13]. Another largely exploited strategy to remove organic matter relies on the application of concentrated hydrogen peroxide [26]. However, its use must be critically evaluated in terms of digestion conditions as treatments with incubation exceeding 48 h with temperatures exceeding 50C, which may degrade plastic polymers like polyethylene and polypropylene [31]. In this context, some authors have recently suggested an effective combined multistep method based on a sequence of enzymatic digestions followed by a short hydrogen peroxide treatment for the removal of organic matter from complex environmental matrices (e.g., wastewater samples). In summary, several promising methods have been tested for extracting, purifying and pre-concentrating plastic materials from sediments and marine biota, all of them having potential limitations. More research is needed to develop a standard protocol for isolating plastics from a range of different environmental matrices, ideally at low cost and without altering plastic properties.
\nOnce isolated, plastic fragments can be tracked and characterized by different analytical techniques. Some are defined as “surface oriented” methods like Raman spectroscopy, Fourier Transformed Infra-Red (FTIR), Scanning Electron Microscopy/Energy Dispersive X-Ray Spectroscopy (SEM-EDS) and environmental scanning electron microscope (ESEM) with an attached X-ray energy dispersive system (ESEM-EDS). Plastic fragments are visually sorted and analyzed coupled with microscopy. However, as discussed above, the use of strong oxidant/acidic agents applied during the extraction from sometimes complex environmental matrices (e.g., organic matter enriched marine sediments, or fat rich marine biota) may induce alteration in the plastic surface like partial dissolution, yellowing and polymer structure disruption leading to erroneous characterization of microparticles. Furthermore, some compounds of natural origin occurring in marine samples (e.g., chitin) have shown spectroscopic properties similar to those of the most recurrent plastic polymers leading to inaccurate polymer characterizations and overall abundance estimation. In addition, these microscopy-based techniques are time consuming and unable to process large numbers of samples. However, significant advances in the automatic and semi-automatic FTIR spectra recognition have been recently presented as promising time saving solutions (Jes recent paper). Alternatively, promising solutions include the Pyrolysis-gas chromatography in combination with mass spectrometry (Pyr-GC-MS) as well as the Thermogravimetric analysis coupled with mass spectrometry (TGA-MS). Pyr-GC-MS in particular can be used to assess the chemical composition of potential microplastic particles by analyzing their thermal degradation products. The polymer origin of particles is identified by comparing their characteristic combustion products with reference pyrograms of known virgin-polymer samples. Py-GC/MS had the advantage of being able to analyze the polymer type and OPA content in one run without using any solvents and with few background contaminations. Additionally, the Pyr-GC/MS method has an appropriate degree of sensitivity for analyzing plasticizers in microplastic particles with limited sample masses. However, although the pyrolysis-GC/MS approach allows for a good assignment of potential microplastics to polymer type it has the disadvantage of being a “destructive” technique as the sample is burned to obtain the pyrolytic products. Furthermore, due to limitations in the quantity of sample loaded in the pyrolysis cup only particles of a certain minimum size can be processed resulting in a lower size limitation of particles that can be analyzed. Each of these methods have their own limitations and advantages, therefore, their combined use, especially for the analysis of complex environmental samples, is a recommended strategy to reduce the effect of interferents in the analysis and obtain reliable results.
\nWith some of the most significant amounts of solid waste generated annually per person (208–760 kg/year), the Mediterranean Sea is one of the world’s areas most affected by litter [32]. The estimated amount is 62 million of macrolitter items floating on the surface of the whole basin [33]. Litter enters the seas from land-based sources, ships and other infrastructure at sea and can travel long distances before being deposited on the seabed or along the coasts. Mean densities of floating microplastics in the Mediterranean Sea of more than 100,000 items/km2 [34] indicate the importance of this threat for the basin. In this context, the Adriatic Sea represents a hot spot for plastic litter both because of peculiarities in its oceanographic conditions as well as the high degree of anthropogenic pressure related to tourism, artisanal and industrial activities coexisting in a narrow area. The Adriatic Sea is an elongated basin, located in the central Mediterranean, between the Italian peninsula and the Balkans, with its major axis in the NW-SE direction. The northern area is very shallow, gently sloping, with an average depth of about 35 m, while the central part is on average 140 m deep, with the two Pomo depressions reaching 260 m. The northern and central parts of the basin are affected by a great number of rivers along the Italian coast, of which the Po river is the most relevant. River discharge and wind stress are the main drivers of the water circulation. West Adriatic Current (WAC), flowing SE along the western coast, and East Adriatic Current (EAC), flowing NE along the eastern coast are the main currents affecting the Adriatic circulation. There are two main cyclonic gyres, one in the northern part and the other in the south. The Bora wind (from NE) causes free sea surface to rise close to the coast enhancing the WAC and the Sirocco wind (from SE), which is the major wind affecting the Adriatic Sea, leads flood events in the shallow lagoons along the basin coast [35]. A vertical thermohaline front parallel to the coast and extending throughout the water mass, divides the coastal waters from the open sea. This retains the materials flowing from rivers and other water sources within the coastal area. A stratification characterizes the water column separating the warmer surface waters with lower salinity from deeper, colder and more saline ones during summer [35].
\nAcross the Mediterranean, but in the Adriatic Sea in particular, there is a continued demand to increase aquaculture production to fulfill the increasing market demand. Mussels, clams, sea bass and seabream production has become a significant source of regional income. Aquaculture was developed to support consumers’ demand for seafood and the methods of production have continued to expand with the growing consumer market. As the need for fish and mussel aquaculture has increased, the development and expansion of aquaculture facilities in coastal and open water locations has increased accordingly. The expansion of the industry and the diversity of materials used to build and maintain aquaculture systems have paralleled the development of synthetic polymers over recent decades. Synthetic fibers offer greater strength and durability than natural fiber ropes; they are cheap, durable and easier to handle compared to their natural counterparts. Most modern aquaculture activities use plastic-based lines, cages, or nets suspended from buoyant or submergible structures (in part made of plastic) and have nanotech plastic-based biofouling and paint applied. Today, tanks, pens, nets, floats, pontoons as well as the pipes of the fish feed supplying systems are made of plastic materials. All plastic material within an aquaculture site is maintained and controlled for chemical degradation, biofouling and corrosion, and is regularly inspected to ensure strength and stability. In the context of global plastic pollution to the oceans, aquaculture may be a contributor to this. However, the estimation of their contribution remains a knowledge gap and lost or derelict gear as well as other possible plastics emissions from aquaculture can be a locally important contributor especially in coastal areas with intensive activity. New reports also point out a potential micro and nanoplastic contamination in wild and cultured seafood products even if the extent of such phenomena is still unknown. There is also concern regarding fisheries as a source of microplastics to the marine environment because both sectors use plastics that may degrade/fragment into microplastics. The coastal areas of Emilia Romagna and the Croatian coast represent sites of intense mussel and fish aquaculture production with hundreds of tons produced yearly. On the other hand, intense fishing activities coexist with a variety of fishing gear and methods being used in industrial and small-scale fisheries. Fishing gear for capture fisheries includes trawl nets, dredges, surrounding nets, lift nets, seine nets, traps, hook and lines. Nets and floats are made from a range of plastics including PP, PET, NyL, PVC, polyamide (PA) and PS.
\nIn oil and gas exploration, drilling fluids based on plastic microbeads were introduced a decade ago. Teflon strengthened particles have been largely applied for drilling purposes internationally. Despite the use of Teflon and other polymers with specific features being used extensively in production, waste treatment processes are not designed for, and give no mention of how to handle plastic particles, so this has clearly not been addressed as an issue in the past. Therefore, there is a substantial lack of information on potential loadings of microplastics used in this sector. To date, few fragmentary studies have addressed this topic. CEFAS’s report entitled, “The discharge of plastic materials during offshore oil and gas operations” suggests that 532 tons of plastics and 7475 tons of “possible plastics” have been released from the UK offshore oil sector. Although knowledge about microplastic from oil and gas extraction activities is limited, it is very likely they represent a potential contributor in the emissions of plastics in aquatic environments, including microplastic and fibers, emphasizing that it should certainly be considered in future source assessments. The mapping of the distribution of rigs and platforms in the Adriatic Sea where tens of oil fields with hundreds of medium sized oil rigs occur, may provide estimations about the geographic distribution of the potential input related to these industrial activities.
\nShips and maritime installations contain many plastic items, like insulation, coating, electrical wiring, furniture and textiles. Ideally, installations should be stripped of all potentially hazardous materials before dismantling. However, plastics items are not identified in the list of harmful materials. Therefore, polymer-based coatings and several kinds of insulation and wiring are rarely stripped.
\nThe distribution of products can contribute to the release of plastics in the environment. Most transferring of stock will occur alongside the transport infrastructure network. However, even if recognized as an important source of pollution, the contribution from releases during transportation, and as is the case for shipping, a map of the main transportation network including roads and harbors is still lacking. Systematic mapping in the Adriatic context has been suggested to improve the understanding of the areas where potential inputs can occur, providing a proxy for the potential intensity for release. The Adriatic Ship Traffic Database also contains information on ports in the Adriatic Sea that could be used to gauge the intensity of port activity to identify which of the port areas could potentially be receiving the largest inputs. Furthermore, the cruise ship industry is pointed out as a significant contributor to the problem of plastic pollution in the Adriatic sea. However, very limited data are available and no specific regulations in place for their plastic waste management and/or assessment of their environmental impact [36].
\nAt a global level, the major challenge to tackle the input of plastic debris from land into the ocean is the lack of adequate waste management in coastal regions with a high and growing population density. Due to a generally high population density in coastal areas of the Adriatic, the pressure resulting from land-based inputs should be relatively high overall. Given such levels of anthropogenic pressure, the lack of, or deficient local waste management systems may lead to locally high inputs linked to industrial or domestic waste management.
\nThere are no studies looking specifically at the leakage and marine input of plastic debris linked to these waste management systems, but ongoing work to quantify and characterize beach litter here points toward potential input from inadequate waste management on the eastern shores of Croatia where the islands of the Quarnero natural park present high loadings of plastic fragments. The composition of the waste accumulated resembles the composition of surveys carried out in the mid-Adriatic region where influence from higher population densities along the coastline is being registered. In addition, a study looking into microplastics near Venice has detected exceptionally high concentrations of small plastic fragments and microplastics in a nearby sandy beach [52]. Though not specified in this report, this exceptionally high concentration of microplastics, including large amounts of plastic fibers and film, could be linked to this location being close to the harbor as well as the lack of waste management facilities. To gain further insight into the potential release of plastics associated with waste management, it would be useful to map the distribution of population density as well as the location of urban agglomerations and settlements as this information will provide an indication of potential localized points of release of plastic waste into the environment. This kind of information is readily available at a sufficient resolution to allow identification of the areas within the Adriatic Sea that need more attention to this potential source of plastic pollution.
\nA rough estimation predicts that 70–80% of marine litter, composed primarily of plastics, originate from inland sources, ending in rivers and oceans. However, inland deposition of MP has not been investigated thoroughly. Potential sources include sewage treatment plants (STPs) and runoff from urban, agricultural, tourist, and industrial areas. As the retention capacity of conventional wastewater treatment processes to MPs appears to be variable in both magnitude and specificity, a characterization of MP emission by STPs and other sources is needed to map major sources of freshwater and terrestrial MPs. A relevant input to the terrestrial ecosystem is by fertilizers obtained by processing sewage sludge, as it typically contains more MPs than liquid effluents. Such fertilizers are frequently used in agriculture, implying a potential accumulation of plastic particles in the soil with continued use, and a systematic examination and quantification has been addressed by several research groups around the world. However, due to runoff, deposited plastic items are most likely transported to rivers and other waterways and ultimately discharged into estuarine and marine environments.
\nThe north of Italy and Croatia represent areas of intense horticultural activities where the agricultural practice of plastic mulching is prevalent. Plastic sheets are used to cover soil in order to preserve moisture, improve fertility and reduce weed infestation. Very often, fragments of plastic films are left behind after use and may accumulate in the soil, further fragmenting to produce nanometric particles. It has been estimated that 125–850 tons of microplastic per million inhabitants are added each year to agricultural soils in Europe, with an annual total of 63,000–430,000 tons of microplastic added to European farmlands. The northern part of Italy and Croatia is an area of significant agricultural and horticultural activities, therefore representing a potential hot spot for the release of plastic fragments in the terrestrial ecosystem. However, due to runoff phenomena these plastic items are most likely transported to rivers and other waterways and ultimately discharged into the estuarine and marine environments.
\nThe first pilot studies of microplastic abundance in confined areas of heavily populated areas like the Oslo fjord noted that a large fraction of particles may be related to city dust (e.g. asphalt and car tires). City dust in urban runoff is known as a significant source of pollution to waterways. Plastics, such as styrene-butadiene, styrene-ethylene-butylene-styrene copolymer, are also used in road materials to make the asphalt more elastic [37]. Another potential contributor to the emissions of plastic fragments is road marking paint as these paints have a variable fraction (1–10%) of thermoplastic component (e.g. styrene-isoprene-styrene, ethylene-vinyl acetate, polyamide and acryl-monomer). On the other hand, the tread of car tires is largely based on styrene-butadiene rubber, a synthetic polymer formulation. Therefore, road dust entering the sea through air or storm water carries a significant fraction of microplastic from road materials, marking paint and car tires.
\nThe description and understanding of the pathways of the entry of marine plastic pollution into the Adriatic Sea is a central element in tracing the pollution back to its sources and developing effective plastic pollution preventing policies. A complete understanding of the input of plastic pollution into the aquatic environment needs to consider the source sectors and the mechanisms of transportation, distribution and partition through different environmental matrices. If the release occurs in the terrestrial environment, rivers and wind or atmospheric circulation constitute the logic pathways. When considering the presence of plastic debris and microplastics in a part of the global Mediterranean Sea there is a need to consider the transfer of marine plastic pollution into the relevant part of the large water bodies through the regional circulation pathway like the Adriatic Sea. The understanding of the input through these pathways is crucial in gauging the relative importance of local sea-based or coastal sources versus remote sources within the Arctic watershed or from other parts of the ocean.
\nThe Adriatic Sea has a limited watershed. The largest rivers in the area are mostly located in the northern sector and include the Po, Adige, Tagliamento, and Arsa rivers. In terms of discharge, the Po River has the largest discharge with 1540 m3/s followed closely by the Adige River with 235 m3/s. The Po Basin is home to some 14 million people and extends over 24% of Italy’s territory. The Po catchment is densely populated and subjected to high anthropogenic pressure heavily anthropized. Indeed, it represents the largest cultivated area in Italy and accounts for one third of national’s agricultural production. The area account also for one of the highest concentrations of economic activities. Such massive river discharges make terrestrial influences particularly strong in the Adriatic Sea. However, to date there is no monitoring of the flux of plastics from rivers into the Adriatic Sea and though it has been identified as a possible pathway, the contribution of riverine discharge to plastic input is expected to be high because these rivers flow through densely populated and anthropized watersheds.
\nIt has been speculated that at the global level much less plastic debris is transported by wind than by rivers [38, 39]. However, wind transport of plastic debris may be significant, particularly in coastal areas dominated by strong periodic winds. Wind may be a significant contributor in lightweight debris distribution. During intense storms wind can mobilize debris that would not normally be available for transport and carry it directly into rivers and the sea. Wind-blown litter is likely to be considerable as the Adriatic Sea is characterized by periodically windy shorelines. Atmospheric circulation has been proven to provide an efficient pathway for the transportation of floating microfibers and small plastic particles in the Mediterranean Sea as well as in other areas [33, 40]. Furthermore, some preliminary transport models tailored to the Adriatic oceanographic conditions, considering the contribution of waves and wind in the surface plastic distribution, define the Adriatic Sea as a highly “dissipative” system with respect to floating plastics with a calculated half-life of floating condition of 43.1 days [41, 42]. The authors conclude by pointing out that by construction the Adriatic coastline may be responsible for the main sink of floating plastic debris.
\nThe contribution of inputs through the movement of marine water masses by currents also needs to be considered in the global distribution model. The Adriatic region is poorly connected to the Mediterranean through the southern edges of the Otranto strait and the Ionian Sea exchanging with the Mediterranean Sea. The exchange of water, and possibly any moving plastic pollution, from and to the Mediterranean Sea has recently been addressed by the modeling work of Liubartseva et al. [40] and partially by the results of Pasquini et al., [40] which pointed out the formation of an accumulation zone corresponding to the three well known gyres located northside, central and in the southern sector of the Adriatic Sea.
\nSome key research projects have recently addressed the need of defining the baseline levels of litter (macro-, meso- and microplastics) in the intertidal areas of beaches within the Adriatic Sea. Blašković et al. [41] investigated the occurrence of plastic debris in several sites of the Natural Park of Telaščica (Croatia). In all analyzed sites, fibers were the most recurring shape (90%) within the identified plastic debris while films where the second most common plastic fragment observed (7%) followed by pellet, foams, granules and unrecognized plastic pieces. Most of the plastic debris belonged to the size fraction from 1 mm and 64 μm (88%) followed by the fraction between 1 and 2 mm (11%). These results confirm previous characterization efforts of Laglbauer et al. [43] in six Slovenian beaches located in the gulf of Trieste (North-East Adriatic Sea). Within this assessment the authors sorted out a total of 5870 macro-debris units, yielding a median density of 1.25 items/m2. The detailed analyses of the processed samples revealed a dominant secondary microplastics source being fibers the 85% of the total observed plastics and a number of 155 particles m2 in the infralittoral zone, and 133 particles m2 on the shoreline. On the Adriatic beaches surveyed, plastic dominated in terms of abundance, followed by paper and other groups. The average density was 0.2 litter items m2, but at one beach it raised to 0.57 items m2. Among plastic, cigarette butts were the most frequently found type of litter, and other plastic items with the highest occurrence were: small fragments, bottles and bottle caps, cutlery, and mesh bags. Their presence is a good indicator of pollution from beach users [44]. Most of the beached marine litter are from land-based sources, but with different sources and contributors. The main source of litter was primarily touristic activities, accounting for 37.9% of found litter which is lower than r the Mediterranean average (52%; [45, 46]). Filter cigarette were the second litter origin, but with a value (25.5%) lower than indicated for the Mediterranean (40%) [44]. The high percentages of in situ deposited litter found in the investigated sites are caused by the high number of visitors, more than 700,000 annually mainly during the touristic season (see i.e., http://statistica.regione.veneto.it; http://imprese.regione.emilia-romagna.it).
\nFew studies have addressed the occurrence of floating plastic debris in the surface water of the Adriatic Sea. Suaria et al. [33] reported by a larger study addressing the Mediterranean Sea and partially the Adriatic sector a clear prevalence of smaller particles. Quantitative estimations collected by a 400 μm net mesh pointed out values ranging from 0.4 ± 0.7 to 1.0 ± 1.8 items/m3. The overall result the study pointed out that, within a total no. of 14,106 scored particles, 26% of all counted particles were smaller than 300 μm while 51% were smaller than 500 μm being the mean abundance of these meso-particles of 0.016 ± 0.028 particles/m2. PE was the predominant form with an overall frequency of 52%, followed by PP (16%) and synthetic paints (7.7%). Polyamides (PA) accounted for 4.7% of all categorized particles which accounted alone for 2%), while PVC, PS and PVA represented equally contributed with 3% of the total. Other less frequent polymers (<1%) included: PET, polyisoprene, poly(vinyl stearate) (PVS), ethylene-vinyl acetate (EVA) and cellulose acetate. Noteworthy the authors concluded that the composition of western Mediterranean samples was dominated by low-density polymers such as polyethylene and polypropylene while the processed Adriatic samples instead were more heterogeneous and rather characterized by a higher presence of paint chips, PS, PVC, PVA and PAs. Within the “Derelict Fishing Gear Management System project – “DeFishGear” project co-funded by IPA-Adriatic Cross-border Cooperation Programme and the European Union, 120 visuals transect surveys were conducted during three cruises, covering a total length of 922.2 km [47]. A total of 1364 macro marine debris objects were observed floating on the Adriatic. The densities of the recorded floating debris were 5.66 items/km2. The authors estimated that the observed floating marine debris was mostly originated from coastal segments close the high-density population cities and major rivers and transported by cyclonic surface circulation until either stranding. They calculated an average time from source to the sighting point of 22.8 days. These outcomes support Carlson and co-workers [48] previous assessment where an average residence time of 22.9 days but with also an average transit times of 20–60 days from a coastal region in the northwest Adriatic to a coastal region in the southwest [47]. The transport pathways, residence times, and probable sources and sinks identified further support with previous studies of the Adriatic Sea surface circulation and marine debris published by Liubartseva et al., [40].
\nData regarding macro- and mesolitter on the sea-floor in the Adriatic Sea are also available from the “SoleMon” Project (Solea Monitoring—Rapido trawl survey in the Northern Adriatic Sea), carried out since 2005 in the Northern and Central Adriatic Sea [49]. Plastic litter was divided by the authors in three sub-categories based on its source: fishing nets, mussel culture debris and other plastic e.g., bottles, plastic glasses, bags. Lost fishing nets and mussel culture debris accounted for 50% of the overall plastic litter collected over the investigated period. The remaining plastic comprised a wide range of objects such as garbage bags, shopping bags, cups, bottles, food packaging, dishes, other kitchen stuffs and industrial packaging [40, 48]. Results of this study indicated that the largest amount of mussel culture debris was found close to the coast and its distribution was constant over the years. These nets might have been accidentally lost/abandoned at sea during the collection and preparation of the product [50]. In the meantime, the fishing nets were found mainly close to the coast within 3 nm. This distribution was explained as fishing nets were mainly set-nets used by small scale fisheries that usually fish not further than 3 nm where there is not trawl fishing that can destroy these nets. A significant contribution of plastic litter found close to the coast was represented by food packaging, plastic bags, bottles and dishes or kitchen tools. The land origin is due to the municipal solid waste [48]. The authors concluded considering that the distribution varied among the years, but the occurrence was mostly related to both the close position of the sampling site to large cities along the coast, where the population density increases during the touristic season as well as the contribution of river [40, 50, 51]. As regards the microliter in the sedimentary environment, a preliminary assessment of microplastics in marine sediments along a coast- off-shore transect in the Central Adriatic was performed by Munari et al. [44]. Plastic fragments recollected from 64 samples were scored, weighted and identified by FTIR. Microplastics ranging 1–30 mm were found in all analyzed samples. The most recurring shapes were filaments-like (69.3%), followed by fragments-like (16.4%), and film-like (14.3%). In term of size distribution, plastic fragments in a range from 1 to 5 mm accounted for 65.1% of debris, while larger fragments (5–20 mm) contributed with the 30.3% of total amount, while larger fragments >20 mm represented the 4.6% of total. Six were the most recurring polymer types: nylon, polyethylene and ethylene vinyl alcohol copolymer. Furthermore, sediments from several sampling sites located in Italy, Slovenia, Croatia, and Greece were also analyzed for plastic debris content by the “DeFishGear” project. Plastic fragments in beach sediments were ranked into large sized particles (1–5 mm) and small microplastic particles (<1 mm). In general, microplastic from 1 to 5 mm ranged from 11 to 710 items/m2. On the other hand, the fraction of smaller size scored from 70 to 6724 items/kg of dry sediments. The mean concentration for all Adriatic region was calculated as 113 ± 101 items/kg for the larger sized fragments and 1133 ± 1271 items/kg of dry sediments for the smaller ones. In detail, the selected Croatian beaches showed considerably greater presence of smaller microplastic per kg of sediment with value of approx. 227 items/kg of sediment while the larger sized fragments sored values approx. Ten times lower (17–28 items/kg of dry sediments). The composition of sorted fragments <1 mm showed the prevalence of plastic fragments as fragments represented approx. 70% of the total while filaments represented the left 29% of the total while a limited amount (1.8 and 0.9%) were film and foams. The chemical characterization of microplastic of the larger particles was performed on foams, pellets, fragments and filaments, while filaments and films were analyzed among the smaller sized particles. Beside the PE and PP in a few percent also PA, PET, PES, PS, PO, nylon and acrylic fibers were present among larger particles, while among the smaller viscose was detected. In the Greek sector data were obtained from three sites: the Halikounas, Issos and Acharavi beaches. The mean concentration of 1–5 mm sized debris varied from 68 items/m2 (Halikounas) to 58 items/m2 (Acharavi) while the small sized fraction of Ø > 1 mm showed values from 19 to 7 items/m2 respectively for Halikounas and Acharavi. The most abundant categories on Halikounas beach were fragments and foam, while on the contrary pellets were the most abundant in Issos and Acharavi beaches. Chemical characterization of fragments, for Halikounas beach were done being both PE and PP the most recurring polymers in the larger particles while PP was the most occurring polymer in the smaller size fraction. The same project also addressed the occurrence in the Italian sector. High amount of small microplastic particles (<1 mm), up to 2526 items/kg of sediment, was found in the Cesenatico area. In the meantime, a limited amount corresponding to 0.56–1.02 items/kg of large particles (1–5 mm) were reported. Overall, 73% of the small microplastic particles were characterized by fragments while the remaining 26% as filaments. On the other hand, the large microplastic particles had different amount of all categories; however, fragments resulted the most abundant category (44%). The chemical identification showed PE as the most abundant material, followed by PP, PO, PES, PS and PAN. In the Slovenian coastline the selected sampling site showed a higher abundance of small microplastic particles (615 items/kg) respect of large microplastic particles (516 items/kg). In detail, the analysis of the small size fraction reported filaments being the predominant type of the microplastic composition, with representation of approx., 76% of the total. The second most common type of microplastic category were fragments and the third were films, with occurrence high as 9.5%. The chemical identification pointed out PE as the most recurring polymer type in the analyzed sediment samples, followed by PP, PET and PVC. Finally, Vianello and co-workers investigated the Venice Lagoon, a fragile estuarine ecosystem dominated by diversified anthropogenic activities, suspected to be a hot spot of plastic debris contamination [53]. Plastic debris of ≤1 mm or less was investigated in sediments collected from 10 sites chosen in shallow areas. Total abundances of plastic fragments varied from 2175 to 672 items/kg with higher concentrations generally found in the inner parts of the Lagoon. PE, PP, ethylene propylene (PEP), polyester (PEst), polyacrylonitrile (PAN), PS, alkyd resin (Alkyd), PVC, polyvinyl alcohol (PVOH) and NyL were identified. PE and PP were the most recurring polymer in the investigated samples which accounted for more than 82% of the total detected plastic debris in the whole sampling area. Among all classified shapes, irregular fragments accounted of the 87% of the total while films (2%) and pellets/granules (1%) were only occasionally recognized [54].
\nThe first report on the harmful effects of plastic debris ingestion on marine species in the Adriatic Sea was published in 1999 [55]. A dead dolphin
The few available studies in the area prove the ubiquity of plastic pollution in the Adriatic Sea. The peculiar oceanographic conditions as well as the high levels of plastic debris recorded in all investigated matrices tend to classify such enclosed area as a hot spot of plastic contamination. Despite the distribution and circulation models appear to accurately estimate fluxes and final fate of marine plastic debris, sinks, sources, fate and residence times of different polymers at sea are the knowledge gaps that need to be addressed in the future to provide concrete info to support concrete actions toward plastic contamination reduction and remediation solutions.
\nThe authors wish to thank The International Research Institute of Stavanger and the National Research Council of Italy- Institute of Marine Science for technical assistance and financial support to publish this work.
\nThe authors declare no conflict of interest.
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Level of education, field of education, and material types of AR used in education and reported educational advantages of AR have been investigated. All articles are categorized according to target groups, which are early childhood education, primary education, secondary education, high school education, graduate education, and others. AR technology has been mostly carried out in primary and graduate education. “Science education” is the most explored field of education. Mobile applications and marker-based materials on paper have been mostly preferred. The major advantages indicated in the articles are “Learning/Academic Achievement,” “Motivation,” and “Attitude”.",book:{id:"6543",slug:"state-of-the-art-virtual-reality-and-augmented-reality-knowhow",title:"State of the Art Virtual Reality and Augmented Reality Knowhow",fullTitle:"State of the Art Virtual Reality and Augmented Reality Knowhow"},signatures:"Rabia M. 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Therefore, this chapter deals with the philosophical systems and paradigms of scientific research, the epistemology, evaluating understanding and application of various theories and practices used in the scientific research. The key components of the scientific research paradigm are highlighted. Theories on the basis of which this research was focused on identification of the level of development of the management culture in order to implement corporate social responsibility are identified, and the stages of its implementation are described.",book:{id:"5791",slug:"management-culture-and-corporate-social-responsibility",title:"Management Culture and Corporate Social Responsibility",fullTitle:"Management Culture and Corporate Social Responsibility"},signatures:"Pranas Žukauskas, Jolita Vveinhardt and Regina Andriukaitienė",authors:[{id:"179629",title:"Prof.",name:"Jolita",middleName:null,surname:"Vveinhardt",slug:"jolita-vveinhardt",fullName:"Jolita Vveinhardt"}]},{id:"74550",title:"School Conflicts: Causes and Management Strategies in Classroom Relationships",slug:"school-conflicts-causes-and-management-strategies-in-classroom-relationships",totalDownloads:2328,totalCrossrefCites:1,totalDimensionsCites:10,abstract:"Conflicts cannot cease to exist, as they are intrinsic to human beings, forming an integral part of their moral and emotional growth. Likewise, they exist in all schools. The school is inserted in a space where the conflict manifests itself daily and assumes relevance, being the result of the multiple interpersonal relationships that occur in the school context. Thus, conflict is part of school life, which implies that teachers must have the skills to manage conflict constructively. Recognizing the diversity of school conflicts, this chapter aimed to present its causes, highlighting the main ones in the classroom, in the teacher-student relationship. It is important to conflict face and resolve it with skills to manage it properly and constructively, establishing cooperative relationships, and producing integrative solutions. Harmony and appreciation should coexist in a classroom environment and conflict should not interfere, negatively, in the teaching and learning process. This bibliography review underscore the need for during the teachers’ initial training the conflict management skills development.",book:{id:"7827",slug:"interpersonal-relationships",title:"Interpersonal Relationships",fullTitle:"Interpersonal Relationships"},signatures:"Sabina Valente, Abílio Afonso Lourenço and Zsolt Németh",authors:[{id:"324514",title:"Ph.D.",name:"Sabina",middleName:"N.",surname:"Valente",slug:"sabina-valente",fullName:"Sabina Valente"},{id:"326375",title:"Prof.",name:"Abílio Afonso",middleName:"Afonso",surname:"Lourenço",slug:"abilio-afonso-lourenco",fullName:"Abílio Afonso Lourenço"},{id:"329177",title:"Dr.",name:"Zsolt",middleName:null,surname:"Németh",slug:"zsolt-nemeth",fullName:"Zsolt Németh"}]},{id:"52475",title:"Teenage Pregnancies: A Worldwide Social and Medical Problem",slug:"teenage-pregnancies-a-worldwide-social-and-medical-problem",totalDownloads:8293,totalCrossrefCites:6,totalDimensionsCites:8,abstract:"Teenage pregnancies and teenage motherhood are a cause for concern worldwide. From a historical point of view, teenage pregnancies are nothing new. For much of human history, it was absolutely common that girls married during their late adolescence and experienced first birth during their second decade of life. This kind of reproductive behavior was socially desired and considered as normal. Nowadays, however, the prevention of teenage pregnancies and teenage motherhood is a priority for public health in nearly all developed and increasingly in developing countries. For a long time, teenage pregnancies were associated with severe medical problems; however, most of data supporting this viewpoint have been collected some decades ago and reflect mainly the situation of per se socially disadvantaged teenage mothers. According to more recent studies, teenage pregnancies are not per se risky ones. A clear risk group are extremely young teenage mothers (younger than 15 years) who are confronted with various medical risks, such as preeclampsia, preterm labor, and small for gestational age newborns but also marked social disadvantage, such as poverty, unemployment, low educational level, and single parenting. In the present study, the prevalence and outcome of teenage pregnancies in Austria are focused on.",book:{id:"5392",slug:"an-analysis-of-contemporary-social-welfare-issues",title:"An Analysis of Contemporary Social Welfare Issues",fullTitle:"An Analysis of Contemporary Social Welfare Issues"},signatures:"Sylvia Kirchengast",authors:[{id:"188289",title:"Prof.",name:"Sylvia",middleName:null,surname:"Kirchengast",slug:"sylvia-kirchengast",fullName:"Sylvia Kirchengast"}]},{id:"58060",title:"Pedagogy of the Twenty-First Century: Innovative Teaching Methods",slug:"pedagogy-of-the-twenty-first-century-innovative-teaching-methods",totalDownloads:8832,totalCrossrefCites:17,totalDimensionsCites:22,abstract:"In the twenty-first century, significant changes are occurring related to new scientific discoveries, informatization, globalization, the development of astronautics, robotics, and artificial intelligence. This century is called the age of digital technologies and knowledge. How is the school changing in the new century? How does learning theory change? Currently, you can hear a lot of criticism that the classroom has not changed significantly compared to the last century or even like two centuries ago. Do the teachers succeed in modern changes? The purpose of the chapter is to summarize the current changes in didactics for the use of innovative teaching methods and study the understanding of changes by teachers. In this chapter, we consider four areas: the expansion of the subject of pedagogy, environmental approach to teaching, the digital generation and the changes taking place, and innovation in teaching. The theory of education, figuratively speaking, has two levels. At the macro-level, in the “education-society” relationship, decentralization and diversification, internationalization of education, and the introduction of digital technologies occur. At the micro-level in the “teacher-learner” relationship, there is an active mix of traditional and innovative methods, combination of an activity approach with an energy-informational environment approach, cognition with constructivism and connectivism.",book:{id:"5980",slug:"new-pedagogical-challenges-in-the-21st-century-contributions-of-research-in-education",title:"New Pedagogical Challenges in the 21st Century",fullTitle:"New Pedagogical Challenges in the 21st Century - Contributions of Research in Education"},signatures:"Aigerim Mynbayeva, Zukhra Sadvakassova and Bakhytkul\nAkshalova",authors:[{id:"201997",title:"Dr.",name:"Aigerim",middleName:null,surname:"Mynbayeva",slug:"aigerim-mynbayeva",fullName:"Aigerim Mynbayeva"},{id:"209208",title:"Dr.",name:"Zukhra",middleName:null,surname:"Sadvakassova",slug:"zukhra-sadvakassova",fullName:"Zukhra Sadvakassova"},{id:"209210",title:"Dr.",name:"Bakhytkul",middleName:null,surname:"Akshalova",slug:"bakhytkul-akshalova",fullName:"Bakhytkul Akshalova"}]},{id:"58894",title:"Research Ethics",slug:"research-ethics",totalDownloads:3371,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Research ethics is closely related to the ethical principles of social responsibility. This research covers a wide context of working with people, so the researchers raised a task not only to gain confidence in the respondents’ eyes, to receive reliable data, but also to ensure the transparency of the science. This chapter discusses the theoretical and practical topics of research, after evaluation of which ethical principles of organization and conducting the research are presented. There is a detailed description of how and what ethical principles were followed on the different stages of the research.",book:{id:"5791",slug:"management-culture-and-corporate-social-responsibility",title:"Management Culture and Corporate Social Responsibility",fullTitle:"Management Culture and Corporate Social Responsibility"},signatures:"Pranas Žukauskas, Jolita Vveinhardt and Regina Andriukaitienė",authors:[{id:"179629",title:"Prof.",name:"Jolita",middleName:null,surname:"Vveinhardt",slug:"jolita-vveinhardt",fullName:"Jolita Vveinhardt"}]}],onlineFirstChaptersFilter:{topicId:"23",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"83053",title:"Apologies in L2 French in Canadian Context",slug:"apologies-in-l2-french-in-canadian-context",totalDownloads:0,totalDimensionsCites:null,doi:"10.5772/intechopen.106557",abstract:"This article presents the results of an analysis of apology strategies in native and non-native French in Canadian context. The data used were obtained through a Discourse Completion Task questionnaire that was completed by a group of native French speakers (FL1) and a group of learners of French as a second language (FL2). The goal was to identify and compare pragmatic and linguistic choices made by both groups when apologizing in three different situations. Several differences and similarities emerged between the two groups regarding the use of exclamations to introduce apologies, direct apologies, indirect apologies, and supportive acts. For instance, it was found that the FL1 speakers used “expressions of regret”, “offers of apology” 15 and “requests for forgiveness” to apologize directly, while the FL2 speaking informants used 16 only “expressions of regret” and “offers of apology”. While the respondents of both groups 17 mostly chose “offers of repair” to apologize indirectly, they displayed divergent preferences 18 regarding the use of other indirect apology strategies. Differences were also documented 19 with respect to the use of intensification devices in direct apologies and the use of supportive acts. Implications of the findings for L2 French pedagogy were also discussed.",book:{id:"11480",title:"Second Language Acquisition - Learning Theories and Recent Approaches",coverURL:"https://cdn.intechopen.com/books/images_new/11480.jpg"},signatures:"Bernard Mulo Farenkia"},{id:"83049",title:"An Ethnographic Study on Sense of a Community: The “Awramba” Experience",slug:"an-ethnographic-study-on-sense-of-a-community-the-awramba-experience",totalDownloads:0,totalDimensionsCites:0,doi:"10.5772/intechopen.105953",abstract:"The study was conducted on “Awramba” Community who are living in “Amhara” region, south “Gondor” Zone, Ethiopia. The general objective of this study was to capture an understanding of sense of community in “Awramba” community. The study tried to answer the following questions: How the community was established? What are the criteria to be part of the community? What are the shared values of social practice that has survived for the test of time? What is the historical background of the “Awramba” Community? The researcher used realist ethnography method to achieve the above objective and to answer the questions. In-depth interview and observational guide techniques were applied to collect reliable data for the study. The observation and in-depth interview data were analyzed qualitatively. The study showed the following themes: Membership criteria of the community are based on adhering to the community norm. They have a strong sense of community based on shared story, cooperative work, marriage and mourning values, religious view, gender equality, commitment to be honest, and solving their problem by themselves. The emotional connection of the “Awramba” community is strengthened by their common celebration of the yearly anniversary of New Year and scheduled meeting.",book:{id:"11429",title:"Sustainability, Ecology, and Religions of the World",coverURL:"https://cdn.intechopen.com/books/images_new/11429.jpg"},signatures:"Nassir-Maru Yesuf"},{id:"83014",title:"Culture: A Pillar of Organizational Sustainability",slug:"culture-a-pillar-of-organizational-sustainability",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.106523",abstract:"Sustainability is a concern that permeates all levels of society and is premised on meeting the needs of the present without compromising the ability of future generations to meet theirs. More recently, policies and research have emerged that guide organizations to align their activities with the broader sustainable development agendas, including cultural issues, not just economic, social, and environmental ones. Culture is the material and immaterial attribute of society. It incorporates social organizations, literature, religion, myths, beliefs, behaviors and entrepreneurial practices of the productive segment, use of technology, and expressive art forms on which future generations depend. Thus, cultural sustainability is a fundamental issue and is configured as the fourth pillar of sustainability, equal to social, economic, and environmental issues, which has to do with the ability to sustain or continue with cultural beliefs and practices, preserve cultural heritage as its entity, and try to answer whether any culture will exist in the future. The importance of cultural sustainability lies in its power to influence people. Their beliefs are in the decisions made by society. Thus, there can be no sustainable development without including culture.",book:{id:"11429",title:"Sustainability, Ecology, and Religions of the World",coverURL:"https://cdn.intechopen.com/books/images_new/11429.jpg"},signatures:"Clea Beatriz Macagnan and Rosane Maria Seibert"},{id:"82949",title:"Corruption and Deterioration of Democracy: The Brazilian Lesson",slug:"corruption-and-deterioration-of-democracy-the-brazilian-lesson",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.106194",abstract:"Although it has emerged, nationally and internationally, as one of the largest investigations against political corruption, Operation Car Wash—at its peak of popular prestige—cleared the path for the political rise of Jair Bolsonaro to the Presidency of the Republic of Brazil. And by doing so, to a certain extent, it paved the way for a set of arbitrary practices that today threaten and weaken the main Brazilian democratic institutions. Brazilian democracy today pays a high price for the Judiciary’s lethargic and condescending response to the unorthodox and illegal practices of Federal Judge Sérgio Moro during the golden years of Operation Car Wash (2014–2018). The lesson that the Brazilian episode brings to the international legal community is that the constant disrespect for the rules of due criminal procedure in large cases of corruption erodes the institutional bases that support the proper confrontation of this type of crime. The pertinent fight against corruption in a democracy can only take place in strict obedience to the law.",book:{id:"11772",title:"Corruption - New Insights",coverURL:"https://cdn.intechopen.com/books/images_new/11772.jpg"},signatures:"Fabio Roberto D’Avila and Theodoro Balducci de Oliveira"},{id:"82903",title:"Walking Accessibility to Primary Healthcare Services: An Inequity Factor for Olders in the Lisbon Metropolitan Area (Portugal)",slug:"walking-accessibility-to-primary-healthcare-services-an-inequity-factor-for-olders-in-the-lisbon-met",totalDownloads:4,totalDimensionsCites:0,doi:"10.5772/intechopen.106265",abstract:"This chapter discusses the walking accessibility to primary healthcare by the olders in Lisbon Metropolitan Area (LMA), Portugal, and its contribution for age-friendly environments as a factor of inequity. Constrains emerged from the collation of the supply approach, represented by service catchment areas based on walking distance time, and the demand approach, through a survey. The location and density of primary health network are a major factor, as it is related to distinct land use patterns within the LMA. The settlement structure influences the potential walkability to primary healthcare. The discrepancy between the potential walking accessibility and the real options is notorious, as olders` choices are diversified in terms of transportation modes and destinations, but mostly keeping relatively short time distances. This phenomenon is also influenced by factors such as personal preference, difficulty to walk, negative perceptions about the surroundings, and insufficient care support. This debate is already an effective concern of local authorities with spatial planning, social and health competences, insofar as solutions in terms of service flexibility and new travel solutions adapted to the specific needs of the olders are a growing reality in the LMA, promoting more age-friendly, health, and inclusive environments, and hence an equitable metropolis.",book:{id:"11479",title:"Social Aspects of Ageing - Selected Challenges, Analyses, and Solutions",coverURL:"https://cdn.intechopen.com/books/images_new/11479.jpg"},signatures:"Eduarda Marques da Costa, Ana Louro, Nuno Marques da Costa, Mariana Dias and Marcela Barata"},{id:"82834",title:"Perspective Chapter: Social Work Education in University Curricula for Sustainable Development",slug:"perspective-chapter-social-work-education-in-university-curricula-for-sustainable-development",totalDownloads:5,totalDimensionsCites:0,doi:"10.5772/intechopen.106246",abstract:"Universities of both global North and South have been changing from the traditional teaching-learning centers to cater to sustainability issues of those countries. Yet, there is a remarkable difference between the universities in the developed and the developing world. It has been found out that the different disciplines of university curricula can be integrated to address and minimize the adverse effects of unsustainability issues. The graduates of the universities will be the future leaders who have to cater to the needs and cope with the challenges of the next generation. There is a dearth of professional social workers to provide the necessary services as numerous catastrophes occur. The global society needs individuals who are equally sound in the knowledge of theory and the experience of practice. As the contemporary global issues become complex, the world needs competent social workers who can serve in different fields of practice. Social work could be the pivotal discipline in understanding common tragedies of the people to apply problem-solving model with the practitioners who are equipped with twenty-first century skills. Social work has to take a transition from a unidisciplinary to a multi- and trans-disciplinary perspective in achieving this objective.",book:{id:"11095",title:"Social Work - Perspectives on Leadership and Organisation",coverURL:"https://cdn.intechopen.com/books/images_new/11095.jpg"},signatures:"Upul Lekamge"}],onlineFirstChaptersTotal:146},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:330,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:9,numberOfPublishedChapters:139,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:123,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:21,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:11,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"August 2nd, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:33,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. His teaching areas are energy metabolism and regulation, integration and organ specialization and metabolic adaptation.",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null},{id:"18",title:"Proteomics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",isOpenForSubmission:!0,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. 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