Types of diapause.
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Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
\n\nThis achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
\n\nWe are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
\n\nThank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
\n\n\n\n\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"5998",leadTitle:null,fullTitle:"Magnetic Sensors - Development Trends and Applications",title:"Magnetic Sensors",subtitle:"Development Trends and Applications",reviewType:"peer-reviewed",abstract:"This book presents an overview of some trends of research and development in the area of magnetic sensors, from materials to applications. A first focus is made on the topics of amorphous micro-wires and thin-film structures and their fabrication, characterization, and application for magnetic sensors based on the effects of giant magneto-impedance (GMI) and magneto-elasticity. A second section deals with the magneto-impedance (MR) sensors, from the development of new materials to sensor implementation and applications. Intended for readers wishing to acquire understanding of the current trends in these areas and comprehension of the issues and the potential of applications of these sensors, this book addresses exciting topics in this field.",isbn:"978-953-51-3648-4",printIsbn:"978-953-51-3647-7",pdfIsbn:"978-953-51-4595-0",doi:"10.5772/66603",price:119,priceEur:129,priceUsd:155,slug:"magnetic-sensors-development-trends-and-applications",numberOfPages:164,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"1dcbfc5df8337f4e2e90b68a218d4197",bookSignature:"Aktham Asfour",publishedDate:"November 29th 2017",coverURL:"https://cdn.intechopen.com/books/images_new/5998.jpg",numberOfDownloads:10974,numberOfWosCitations:22,numberOfCrossrefCitations:23,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:36,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:81,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"November 23rd 2016",dateEndSecondStepPublish:"January 14th 2017",dateEndThirdStepPublish:"September 16th 2017",dateEndFourthStepPublish:"October 16th 2017",dateEndFifthStepPublish:"December 16th 2017",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"36070",title:"Dr.",name:"Aktham",middleName:null,surname:"Asfour",slug:"aktham-asfour",fullName:"Aktham Asfour",profilePictureURL:"https://mts.intechopen.com/storage/users/36070/images/6505_n.jpg",biography:"Dr. Aktham Asfour is currently an associate professor at the University Grenoble Alpes (UGA), France. He received the Engineering and the PhD degrees from Grenoble INP (Institute of Engineering) in 1998 and 2002, respectively. Since 2010, he has been carrying out his research in Grenoble Electrical Engineering Lab (G2Elab). His main interests include magnetic sensors and applications as well as the systems of nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) at very- and ultralow magnetic field, including portable and mobile systems. Between 2004 and 2010, he has been with the Grenoble Institute of Neuroscience (GIN) in Grenoble where he also carried out research in radiofrequency coils and electronics for the NMR and MRI of hyperpolarized gases.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Laboratoire de Génie Electrique de Grenoble",institutionURL:null,country:{name:"France"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"758",title:"Sensor Technology",slug:"electrical-and-electronic-engineering-sensor-technology"}],chapters:[{id:"56809",title:"Magnetic Fibers",doi:"10.5772/intechopen.70534",slug:"magnetic-fibers",totalDownloads:1353,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The magnetic sensors based on soft magnetic effects of amorphous fibers are one of the highlights in scientific research in recent years. The amorphous fibers not only have excellent mechanical properties but also have unique magnetic properties, such as high permeability. As a result, sensors made of this kind of material can show the characteristics of sensitivity and durability. The processing and their advantages and disadvantages are mainly introduced in the chapter, and the properties reported in recent years are also summed up, including mechanical behavior, magnetic properties and shape-memory effects.",signatures:"Rui Xuan Li and Yong Zhang",downloadPdfUrl:"/chapter/pdf-download/56809",previewPdfUrl:"/chapter/pdf-preview/56809",authors:[{id:"203937",title:"Prof.",name:"Yong",surname:"Zhang",slug:"yong-zhang",fullName:"Yong Zhang"},{id:"206541",title:"Ms.",name:"Rui Xuan",surname:"Li",slug:"rui-xuan-li",fullName:"Rui Xuan Li"}],corrections:null},{id:"56713",title:"Wireless Stress Sensor Based on Magnetic Microwires",doi:"10.5772/intechopen.70354",slug:"wireless-stress-sensor-based-on-magnetic-microwires",totalDownloads:1498,totalCrossrefCites:2,totalDimensionsCites:4,hasAltmetrics:0,abstract:"The development of wireless sensors and biosensors is a topic of great current interest. Amorphous magnetoelastic microwires are perfect candidates to be used as sensing elements based on two important properties, that is, magnetoelastic resonance and high-frequency giant magnetoimpedance. It was observed that such microwires present the key feature of performing magnetoelastic resonance, at the kHz range of frequency, in the absence of applied field. This fact, in addition to their small size, gives the microwires unique advantages over the widespread ribbons, currently in use as magnetoelastic sensors. The frequency, amplitude, and damping of the vibration give information of the sensor environment. On the other side, the microwire reflectivity in the microwave range can be modulated by means of magnetoimpedance effect. The maximum-induced electric current, as well as the maximum ac modulation, occurs for frequencies determined by the microwire length. The modulation also varies as a function of the dc-applied field and applied stress.",signatures:"Pilar Marín",downloadPdfUrl:"/chapter/pdf-download/56713",previewPdfUrl:"/chapter/pdf-preview/56713",authors:[{id:"120139",title:"Dr.",name:"Pilar",surname:"Marin",slug:"pilar-marin",fullName:"Pilar Marin"}],corrections:null},{id:"56475",title:"Thin-Film Magneto-Impedance Sensors",doi:"10.5772/intechopen.70084",slug:"thin-film-magneto-impedance-sensors",totalDownloads:1559,totalCrossrefCites:5,totalDimensionsCites:9,hasAltmetrics:0,abstract:"We review the state-of-the-art of thin films displaying the magneto-impedance (MI) effect, with focus on the aspects that are relevant to the successful design and operation of thin film-based magnetic sensors. After a brief introduction of the MI effect, the materials and geometries that maximize the performance, together with the measurement procedure for their characterization, are exposed. A nonexhaustive survey of applications is included, mostly with the aim of displaying the capabilities of thin film structures in the field of magnetic sensing, and the variety of topics covered by them. A special emphasis is made on some concepts that are not commonly treated in the literature, such as the influence of the measuring circuit on the magneto-impedance ratio, the geometry optimization by means of numerical simulation by finite element methods, or noise measurements on thin films. Additionally, a brief description of the patterning procedure by photolithography is included, since the major advantage of thin film sensors over other types of magneto-impedance materials as ribbons or wires is the possibility of patterning the sensible element in micrometric shapes, and most of all, their easy integration with the interface microelectronic circuitry.",signatures:"Alfredo García-Arribas, Eduardo Fernández and David de Cos",downloadPdfUrl:"/chapter/pdf-download/56475",previewPdfUrl:"/chapter/pdf-preview/56475",authors:[{id:"203261",title:"Dr.",name:"Alfredo",surname:"García-Arribas",slug:"alfredo-garcia-arribas",fullName:"Alfredo García-Arribas"},{id:"208996",title:"Dr.",name:"Eduardo",surname:"Fernández",slug:"eduardo-fernandez",fullName:"Eduardo Fernández"},{id:"208997",title:"Dr.",name:"David",surname:"De Cos",slug:"david-de-cos",fullName:"David De Cos"}],corrections:null},{id:"56624",title:"Engineering Magnetoresistance in MnxGe1−x System for Magnetic Sensor Application",doi:"10.5772/intechopen.70206",slug:"engineering-magnetoresistance-in-mnxge1-x-system-for-magnetic-sensor-application",totalDownloads:1300,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"In 2007, a Nobel Prize is awarded to Dr. Albert Fert and Peter Grünberg for their contribution in giant magnetoresistance (GMR) effect. The magnetic head based on GMR effect has significantly increased the storage density in the hard disk drive (HDD) and brought the coming of the digital age. Besides, the rapid development of GMR sensor has opened a wide and promising range of applications, including the aspects in automobile, traffic monitor, biomedicine, and space, etc. As continuously extending the market, it needs GMR sensor with much lower cost, smaller size, higher sensitivity, and compatibility with the CMOS technology. In light of that, we give a review about the recent progress of the MR effect in MnxGe1−x system, which refers to the material growth and magnetic and MR property. Through engineering the MnxGe1−x structure, it could realize the transition from negative to positive MR, geometric-enhanced giant MR, and electric-field controlled MR. The fact of well-designed MR effect and high compatibility with Si technology brings a high potential and advantage for fabricating MnxGe1−x-based MR sensors, which could be widely used in magnetic head and biomedical sensors, among others, with the superiority of much lower manufacturing cost, lower power dissipation, higher integration density, and higher sensitivity.",signatures:"Tianxiao Nie, Weisheng Zhao and Kang L. Wang",downloadPdfUrl:"/chapter/pdf-download/56624",previewPdfUrl:"/chapter/pdf-preview/56624",authors:[{id:"183741",title:"Dr.",name:"Kang L.",surname:"Wang",slug:"kang-l.-wang",fullName:"Kang L. Wang"},{id:"202494",title:"Dr.",name:"Tianxiao",surname:"Nie",slug:"tianxiao-nie",fullName:"Tianxiao Nie"},{id:"209117",title:"Prof.",name:"Weisheng",surname:"Zhao",slug:"weisheng-zhao",fullName:"Weisheng Zhao"}],corrections:null},{id:"56492",title:"Double Perovskite Sr2FeMoO6: A Potential Candidate for Room Temperature Magnetoresistance Device Applications",doi:"10.5772/intechopen.70193",slug:"double-perovskite-sr2femoo6-a-potential-candidate-for-room-temperature-magnetoresistance-device-appl",totalDownloads:1663,totalCrossrefCites:2,totalDimensionsCites:5,hasAltmetrics:0,abstract:"The family of double perovskites first received attention in the 1960s, but the discovery of low field magnetoresistnace (LFMR) and half metallicity of the Sr2FeMoO6 (SFMO) compound was made by Kobayashi et al. in 1998. A fully spin polarized half-metal SFMO (Tc > 400) with excellent magnetoresistance response relatively at small applied fields and high temperatures makes SFMO an ideal candidate for room temperature spintronics applications. Primarily, most of the research work on double perovskites SFMO has been focused on bulk ceramic samples and aimed to understand their structural, magnetic, and magnetotransport properties, along with correlation among them. A material such as SFMO that exhibits a large decrease in resistivity and magnetically order well above room temperature is necessary for the advancement of spintronic devices. If the bulk properties observed could be reproduced in thin films, industrially produced SFMO-based spintronic devices could become a reality. Therefore, the purpose of this chapter is to present the detailed background and descriptions of the double perovskite Sr2FeMoO6 (SFMO) thin films and heterostructures with main emphasis to improve or achieve room temperature magnetoresistance properties especially for room temperature magnetoresistive device applications.",signatures:"Nitu Kumar, Geetika Khurana, Ram S. Katiyar, Anurag Gaur and R.\nK. Kotnala",downloadPdfUrl:"/chapter/pdf-download/56492",previewPdfUrl:"/chapter/pdf-preview/56492",authors:[{id:"151538",title:"Prof.",name:"Ram S.",surname:"Katiyar",slug:"ram-s.-katiyar",fullName:"Ram S. Katiyar"},{id:"201411",title:"Dr.",name:"Nitu",surname:"Kumar",slug:"nitu-kumar",fullName:"Nitu Kumar"},{id:"204317",title:"Dr.",name:"Geetika",surname:"Khurana",slug:"geetika-khurana",fullName:"Geetika Khurana"},{id:"210383",title:"Dr.",name:"Anurag",surname:"Gaur",slug:"anurag-gaur",fullName:"Anurag Gaur"},{id:"210384",title:"Dr.",name:"Ravinder",surname:"Kotnala",slug:"ravinder-kotnala",fullName:"Ravinder Kotnala"}],corrections:null},{id:"56839",title:"Giant Magnetoresistance Sensors Based on Ferrite Material and Its Applications",doi:"10.5772/intechopen.70548",slug:"giant-magnetoresistance-sensors-based-on-ferrite-material-and-its-applications",totalDownloads:1983,totalCrossrefCites:5,totalDimensionsCites:9,hasAltmetrics:0,abstract:"In recent decades, new magnetic sensors based on giant magnetoresistance (GMR) have been studied and developed intensively. GMR materials have great potential for next-generation magnetic field sensing devices. The GMR material has many attractive features, for example, its electric and magnetic properties can be varied in a very wide range, low power consumption, and small size. Therefore, GMR material has been developed into various applications of sensor based on magnetic field sensings, such as magnetic field sensor, a current sensor, linear and rotary position sensor, data storage, head recording, nonvolatile magnetic random access memory, and biosensor. In this chapter, the recent development of a GMR thin-film–based ferrite material will be reviewed. Furthermore, recent and future trend application of GMR sensor will be discussed.",signatures:"Mitra Djamal and Ramli Ramli",downloadPdfUrl:"/chapter/pdf-download/56839",previewPdfUrl:"/chapter/pdf-preview/56839",authors:[{id:"204475",title:"Prof.",name:"Mitra",surname:"Djamal",slug:"mitra-djamal",fullName:"Mitra Djamal"},{id:"210205",title:"Dr.",name:"Ramli",surname:"Ramli",slug:"ramli-ramli",fullName:"Ramli Ramli"}],corrections:null},{id:"56720",title:"AMR Sensor and its Application on Nondestructive Evaluation",doi:"10.5772/intechopen.70334",slug:"amr-sensor-and-its-application-on-nondestructive-evaluation",totalDownloads:1620,totalCrossrefCites:9,totalDimensionsCites:9,hasAltmetrics:0,abstract:"To improve the performance of anisotropic magnetoresistance (AMR) sensor, a low-noise driving circuit for the AMR sensor was developed and the magnetic field noise spectral densities of 12 pT/root(Hz) at 1 kHz and 20 pT/root(Hz) at 100 Hz were achieved. The driving circuit could operate in amplifier mode or feedback mode. For the driving circuit with feedback, the distortion of the system was reduced and the AMR sensor was suitable for the applications in the environment without shielding. The Set/Reset method was used to reduce the low frequency noise of the AMR sensor. Due to the low noise of AMR sensor, the eddy current testing (ECT) system with the AMR sensor had the advantage of detecting deep and small defects in metal structures. The dual frequency ECT system was developed to reduce the influence of lift-off variance. Using the ECT system with the AMR sensor, we successfully detected the small defects in the combustion chamber of liquid rocket.",signatures:"Dongfeng He",downloadPdfUrl:"/chapter/pdf-download/56720",previewPdfUrl:"/chapter/pdf-preview/56720",authors:[{id:"202714",title:"Dr.",name:"Dongfeng",surname:"He",slug:"dongfeng-he",fullName:"Dongfeng He"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"2405",title:"Smart Actuation and Sensing Systems",subtitle:"Recent Advances and Future Challenges",isOpenForSubmission:!1,hash:"e0ba43135d522bb9ad308c72f3fc932b",slug:"smart-actuation-and-sensing-systems-recent-advances-and-future-challenges",bookSignature:"Giovanni Berselli, Rocco Vertechy and Gabriele Vassura",coverURL:"https://cdn.intechopen.com/books/images_new/2405.jpg",editedByType:"Edited 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In this way, scholars, postgraduates, students, and others will be able to become acquainted with modern scientific research into menstrual cycle. For others, this book will be the impetus for further research in this area.",isbn:"978-1-78985-673-6",printIsbn:"978-1-78923-934-8",pdfIsbn:"978-1-78985-674-3",doi:"10.5772/intechopen.76123",price:119,priceEur:129,priceUsd:155,slug:"menstrual-cycle",numberOfPages:146,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"f3e1135f2d45bbaafe6e61b252108d05",bookSignature:"Olena Ivanivna Lutsenko",publishedDate:"June 12th 2019",coverURL:"https://cdn.intechopen.com/books/images_new/7494.jpg",keywords:null,numberOfDownloads:10101,numberOfWosCitations:1,numberOfCrossrefCitations:3,numberOfDimensionsCitations:11,numberOfTotalCitations:15,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 13th 2018",dateEndSecondStepPublish:"May 3rd 2018",dateEndThirdStepPublish:"July 2nd 2018",dateEndFourthStepPublish:"September 20th 2018",dateEndFifthStepPublish:"November 19th 2018",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"4 years",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:5,editedByType:"Edited by",kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"225667",title:"Mrs.",name:"Olena",middleName:null,surname:"Lutsenko",slug:"olena-lutsenko",fullName:"Olena Lutsenko",profilePictureURL:"https://mts.intechopen.com/storage/users/225667/images/system/225667.jpg",biography:"Olena Lutsenko obtained her Ph.D. in 2021. Since 2015, she has had the opportunity to work on a departmental research topic on the fundamentalization of natural education for sustainable development. She is currently working on scientific topics at Oleksandr Dovzhenko Hlukhiv National Pedagogical University, Ukraine, and teaches medical and biological disciplines and features of teaching methods of these disciplines. She has published more than seventy-nine scientific papers, including five textbooks and five monographs. Her recent research focuses on the peculiarities of preparing future teachers for their professional activities, as well as on the introduction of modern educational technologies in educational activities.",institutionString:"Oleksandr Dovzhenko Hlukhiv National Pedagogical University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"3",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"Oleksandr Dovzhenko Hlukhiv National Pedagogical University",institutionURL:null,country:{name:"Ukraine"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"1069",title:"Reproductive Endocrinology and Infertility",slug:"obstetrics-and-gynecology-reproductive-endocrinology-and-infertility"}],chapters:[{id:"66096",title:"Introductory Chapter: Regulation of Ovarian-Menstrual Cycle as a Systemic Problem of Physiology of Humans",slug:"introductory-chapter-regulation-of-ovarian-menstrual-cycle-as-a-systemic-problem-of-physiology-of-hu",totalDownloads:942,totalCrossrefCites:0,authors:[{id:"225667",title:"Mrs.",name:"Olena",surname:"Lutsenko",slug:"olena-lutsenko",fullName:"Olena Lutsenko"}]},{id:"64704",title:"Normal Menstrual Cycle",slug:"normal-menstrual-cycle",totalDownloads:3181,totalCrossrefCites:2,authors:[{id:"250273",title:"Associate Prof.",name:"Patricio",surname:"Barriga",slug:"patricio-barriga",fullName:"Patricio Barriga"}]},{id:"63668",title:"Pre Menstrual Syndrome",slug:"pre-menstrual-syndrome",totalDownloads:1178,totalCrossrefCites:0,authors:[{id:"213063",title:"Prof.",name:"Rosemary",surname:"Ogu",slug:"rosemary-ogu",fullName:"Rosemary Ogu"},{id:"256597",title:"Dr.",name:"Avwebo",surname:"Otoide",slug:"avwebo-otoide",fullName:"Avwebo Otoide"},{id:"256598",title:"Dr.",name:"Preye",surname:"Fiebai",slug:"preye-fiebai",fullName:"Preye Fiebai"}]},{id:"62617",title:"Menstrual Cycle and Physical Effort",slug:"menstrual-cycle-and-physical-effort",totalDownloads:1349,totalCrossrefCites:1,authors:[{id:"253369",title:"Dr.",name:"Magdalena",surname:"Wiecek",slug:"magdalena-wiecek",fullName:"Magdalena Wiecek"}]},{id:"63848",title:"Premature Ovarian Insufficiency",slug:"premature-ovarian-insufficiency",totalDownloads:1129,totalCrossrefCites:0,authors:[{id:"254629",title:"M.D.",name:"Abdelhamid",surname:"Benmachiche",slug:"abdelhamid-benmachiche",fullName:"Abdelhamid Benmachiche"},{id:"265542",title:"Prof.",name:"Amel",surname:"Dammene Debbih",slug:"amel-dammene-debbih",fullName:"Amel Dammene Debbih"}]},{id:"64960",title:"Secretory Phase and Implantation",slug:"secretory-phase-and-implantation",totalDownloads:1302,totalCrossrefCites:0,authors:[{id:"255491",title:"Dr.",name:"Courtney",surname:"Marsh",slug:"courtney-marsh",fullName:"Courtney Marsh"}]},{id:"63858",title:"Standardization of Menstrual Cycle Data for the Analysis of Intensive Longitudinal Data",slug:"standardization-of-menstrual-cycle-data-for-the-analysis-of-intensive-longitudinal-data",totalDownloads:1020,totalCrossrefCites:0,authors:[{id:"251727",title:"Dr.",name:"Sherry",surname:"Stewart",slug:"sherry-stewart",fullName:"Sherry Stewart"},{id:"251728",title:"BSc.",name:"Kayla",surname:"Joyce",slug:"kayla-joyce",fullName:"Kayla Joyce"}]}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"259492",firstName:"Sara",lastName:"Gojević-Zrnić",middleName:null,title:"Mrs.",imageUrl:"https://mts.intechopen.com/storage/users/259492/images/7469_n.png",email:"sara.p@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. 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Certain creatures possess the properties of eternal youth and deathlessness. These include Turritopsis (a species of small jellyfish) and planarians. Old Turritopsis starts a degeneration to transform into polyps and thereby achieves a perpetual life cycle [1]. The old cells contained in an old imago change into young cells when the imago transforms into a polyp. The polyp then starts to grow until it reaches the imago stage. As the Turritopsis can repeat this cycle forever, it can be considered to exist in a state of deathlessness. Planarians possess a special property of not growing older. They contain numerous stem cells throughout their bodies and every portion of their body can reproduce [2]. Planarians may therefore be an ageless organism. The property of agelessness is also present in humans. The case of Brooke Greenberg, an American woman who could not grow older after developing highlander syndrome, was the subject of a number of news reports in Japan a few years ago [3]. Moreover, there are several reported cases of female patients who developed highlander syndrome; however, the veracity of these reports is unclear, and the gene that causes the syndrome has not been elucidated. The development of highlander syndrome in human patients suggests that humans might possess the genes that enable the property of eternal youth.
\nThe actualization of eternal youth is a long-held dream, and numerous studies have been performed to elucidate the mechanisms of aging and to achieve eternal youth. Consequently, recent studies have partially elucidated the process of aging and proposed several anti-aging or rejuvenation procedures; however, the studies are currently in the middle stage, and the key elements of the aging process have yet to be elucidated. The purpose of this chapter is to present an overview of the recent topics on cellular aging and rejuvenation to provide an outline of the content in this book. This chapter also complements the chapters that follow; in which researchers introduce topics related to aging and rejuvenation.
\nCultured human cells become older through repeated cell division. Cells eventually stop dividing when they reach the critical passage number, the Hayflick limit [4]. Blackburn [5] and Greider discovered that the telomeres function as a clock by marking the passage of time in cells. The molecular mechanism of aging, which is described below [6–8], was elucidated through this discovery.
\nRNA primer is degraded and exchanged to DNA with DNA polymerase I followed by replication with DNA polymerase III. However, the RNA primer present at the terminal portion of telomeres cannot be exchanged to DNA. As a result, approximately 100 sequence bases are shortened in every replication. The shortening of telomeres acts as a fuse and decides the limit of cell division. The length limit (M1 period) in human fibroblasts is approximately 5 kb, but most cells in elderly humans do not reach the M1 period [9]. Moreover, mouse telomeres are of sufficient length, even in old mice, due to the expression of telomerase. Otherwise, cells in the M1 period can continue to undergo cell division by transforming with T antigen [10]. In such cells, the shortening of telomeres continues, and cell division is finally stopped again by the fusion of mutual terminals (the M2 period) or apoptosis. Telomeres are therefore protected from further shortening by a safe limit (the M1 period).
\nThe pathway from telomere shortening to the cessation of cell division has also been elucidated [7]. A telomere is formed by both double-stranded DNA, which is made by repeated sequences (“TTAGGG” in the mammals), and single-strand DNA (G-tail) of similar sequences that exist at the terminal portion. A telomere has to construct the T-rope structure to avoid degradation by DNA degrading enzymes. This structure gives the first signal to initiate the progression of cell division. The shortening of telomeres causes the obstruction of the T-rope structure and signals certain proteins, including telomeric repeat-binding factor 2 (TRF2), AMP kinase and histone deacetylase, to delay or stop cell division. The detection of the signal by AMP kinase activates p53 and/or p21 proteins and inhibits the work of the cyclin-dependent kinase (CDK) complex. Finally, the inhibition of the CDK complex causes cell division to stop at the Go period, because CDK is a control switch that determines whether cell division should be promoted.
\nMitochondria also have a role in the aging process [11, 12]. Mitochondria produce ATP using the electron transport chain pathway where reactive oxygen species (hydrogen peroxide, hydroxyl radical and hydroperoxyl radical) are produced. Reactive oxygen species are often leaked through the mitochondrial inner membrane, damage DNA, proteins, and lipids. It has been confirmed by the experiments with nematodes [13] that reactive oxygen species promote aging. For instance, the mutation of the mev-1 gene in nematodes was found to result in a shorter life span, because the gene disruption caused a defect in the electron transport chain and an increase in the level of reactive oxygen species. Conversely, a mutation of the age-1 gene in nematodes caused the enhancement of catalase activity which degraded hydrogen peroxide and resulted in a prolonged life span.
\nRecent studies have suggested an interaction between mitochondrial dysfunction and telomere shortening because of the following process [12, 14, 15]. The shortening of telomeres causes the activation of the p53 protein, the activation of which inhibits the activities of PGC-1α and β, which induce the activation of mitochondria. This inhibition finally causes a decrease in many important mitochondrial activities and the progression of the aging process. On the contrary, an increase in the level of active oxygen species due to mitochondrial dysfunction often causes the oxidation of telomeres; the numerous guanine repeats on telomeres cause them to react easily with oxygen. In telomere DNA, oxidation disturbs the combination of TRF2 with telomere DNAs and the normal T-rope structure, which is the first signal that cell division cannot proceed. Thus, under high concentrations oxygen, the telomeres in human cells are rapidly shortened and cell growth is inhibited.
\nStudies using both old cells (M1 period) and young cells (Low PDL) have suggested other causes of the aging. When cell fusion occurs between a young cell and an old cell from which the nucleus has been removed, the cell division of the fused cell is inhibited. However, when old cells that were previously treated with a protein synthesis inhibitor are used, growth is not inhibited. Moreover, when the cell membrane of old cells or mRNAs of cells stopped at the Go period, respectively, are injected into younger cells, cell division stops or DNA replication is inhibited. These results suggest that some proteins, mRNAs and/or cell membranes that are present in older cells gradually accumulate with every cell division and promote the aging process. The genes corresponding to such compounds have also been screened [16, 17]. Some genes (gas, gadd, mot1, and hic-5) have been cloned. Unfortunately, they were not the most important genes for controlling the aging process. Recently screening has been performed using RNA, and some promising genes have been identified [18, 19].
\nInformation that is important for elucidating the aging process in humans can be obtained from the genes that cause premature senility syndrome. Five types of helicases (RecQL1, BLM, WRN RecQL4/RTS, and RecQL5) that untangle DNA chains exist in humans. The change of the proteins to abnormal sequences causes premature senility syndromes [20]. Werner, Bloom, and Rothmund–Thomson syndromes are caused by abnormal structures of the WRN, BLM, and RecQL4/RT proteins, respectively. The WRN protein, which is related to the replication, restoration, transcription, and stabilization of DNA or telomeres, is remarkable. In the case of patients of Werner syndrome, the onset of symptoms occurs after patients stop growing at approximately 10 years of age. In Werner syndrome, the aging process advances much faster than in normal individuals. Patients show normal nerve and immune systems but possess unusual chromosomes. The WRN gene was expected to become a target of aging in normal individuals, because with the exception of the speed at which aging advances, the symptoms are similar to the normal aging process. However, WRN knockout mice do not show premature senility syndrome, whereas WRN and TERC knockout mice do [21]. Further investigation is necessary to improve our understanding of the relationship between WRN and the aging process.
\nThe other remarkable premature senility syndrome is Hutchinson–Gilford progeria syndrome (HGPS), which is caused by partial loss of the lamin A protein [22]. HGPS patients are normal at birth. HGPS develops at 6–18 months of age; the average life span of an HGPS patient is 13 years. Lamin A exists inside a nuclear membrane and supports the structure of the membrane. It is changed to a farnesylated version to perform nuclear translocation, and farnesylated lamin A is related to both the replication and transcription of DNA and signal transduction. The unusual farnesylated lamin A that is found in HGPS patients is called “progerin” [23]. Progerin accumulates and inhibits translocation, and the inhibition causes the aging of cells. In normal individuals, progerin gradually accumulates in the skin cells due to aging. Progerin is therefore a target of treatments to delay the aging process.
\nIn humans, although telomerase can make telomeres longer, most types of cells (including fibroblasts, spanchnic cells, and nerve cells) show very low telomerase activity [24]. In contrast, germ and cancer cells, which actively perform cell division, show high telomerase activity and long telomeres. This suggests that the cells may be rejuvenated if telomerase can be activated [25–27].
\nStudies on the fibroblasts and mice that express telomerase reverse transcriptase (TERT) gene by transformation have supported this hypothesis [28–30]. For example, a human OSMU36-T2 fibroblast showing high telomerase activity was obtained by transforming the TERT gene into an OSMU36 fibroblast. The telomere length and the telomerase activity of OSMU36-T2 were several times higher than those observed in OSMU36 fibroblast, and many characteristics including the cell size, growth rate, and gene expression were similar to those observed in young fibroblasts [28]. Moreover, the life span of a TERT knockout mouse, which was produced by Harvard University, was much shorter (only 6 months) than normal mice due to the rapid shortening of the telomeres. When telomerase was activated in the knockout mice, neurons were formed and rejuvenation was found in some portions [29, 30]. Thus, it may be possible to initiate cellular rejuvenation in individuals as well as cultured cells through the activation of telomerase.
\nTelomerase activity and telomere length are affected by lifestyle [31, 32]. Researchers at California University group examined the effects of food, exercise, and psychological stress on the telomere length in 35 males [31]. The members of one group continued to consume vegetables as a staple food, to perform adequate exercise and to decrease psychological stress through self-control for 5 years. As a result, their telomeres were longer than the members of the control group. Other researchers investigated the effects of exercise. The results suggested that the athletes who ran more than 40 km every day were 16 years younger in telomere length. Moreover, telomerase can be activated by some chemical compounds, which are expected to have applications as rejuvenation drugs. For instance, the rate at which telomeres shorten is accelerated by high serum concentrations of cholesterol, because cholesterol hastens cell division. Thus, mononucleosis patients who continuously took statin (an anticholesteremic agent) showed higher telomerase activity and longer telomeres than the patients who did not take statin [33]. Several years ago, a compound named TA-65, which was isolated from the root of the Hedysarum, was screened as a telomerase activating compound [34], and the rejuvenation effect of TA-65 was examined in mice.
\nAs telomerase activity can be controlled by lifestyle and some compounds, the enhancement of telomerase activity may become an effective treatment to promote cellular rejuvenation. However, the enhancement of telomerase activity may also cause the activation or induction of cancer cells, because high telomerase activity is one of the characteristics of cancer cells [35–37]. Further studies to determine whether the activation of telomerase can truly induce a rejuvenation condition without the risk of cancer cell activation will be necessary before it can be used in supplements and drugs.
\nLeucocytes secrete reactive oxygen species to protect against psychological stress in social life or physical stresses including atmospheric pollutants, UV, and viruses. The excessive production of reactive oxygen species promotes aging (as described in Section 2.2.). Although catalase, superoxide dismutase (SOD), and glutathione peroxydase can remove such reactive oxygen species in humans, catalase, and SOD activities gradually decrease due to aging. Thus, reactive oxygen species cannot be sufficiently removed in elderly individuals. In other words, the aging process can be delayed or remediated if the excessive production of reactive oxygen species is prevented.
\nAntioxidants, such as vitamins and polyphenols, are effective in removing active oxygen species, and their anti-aging effects have been studied for many years [38, 39]. The studies suggest that the oxidation of cultured cells can be inhibited by antioxidants and that mice that continuously took antioxidants showed lower oxidation and a longer life span than controls. Recent studies in humans, however, suggested that the anti-aging effects are doubtful. For example, in experiments in which healthy human subjects took β-carotene or vitamins for a long period of time, the ratio of depth was not decreased. Certain amounts or components of antioxidants may be required to make the effects of antioxidants prominent in humans.
\nAnti-aging hormones have remarkable potential as anti-aging or rejuvenation treatments. There are several candidate compounds, including klotho, sirtuin, Bach1, Clk-1, and polyamines [40–46]. The klotho gene is mainly expressed in the nervous system to control the calcium concentration in blood; klotho works as a controller of homeostasis, although the precise work of the protein has not been sufficiently elucidated [40, 41]. Klotho knockout mice develop many symptoms related to aging, such as arteriosclerosis, osteoporosis, motor impairment and have a shortened life span. Conversely, klotho knock-in mice have a life span that is several years longer [42]. Thus, klotho is a promising target in rejuvenation.
\nHistone deacetylases (sirtuin) is another candidate protein [43, 44]. Sirtuin works as an energy economizing hormone to prolong the life span. Sirtuin 1, 6 and 7 knockout mice showed faster aging and a shorter life span. Hibernation, which represents a state of extreme energy limitation, extended the life spans of yeasts, rematodes, and mice. One reason for the prolongation of the life span is that the amount of excess active oxygen is decreased by the energy limitation; thus, the promotion of the aging process by undesirable oxidation is inhibited. Another cause is the activity of sirtuin. Sirtuin 2 is activated by the decrease of NAD, which is caused by energy limitation. The activation of sirtuin 2 increases the deacetylation of histones to silence the genes, and the economizing of energy prolongs life span.
\nThe following monkey experiments were performed to estimate the action of sirtuin in relation to energy limitation. The Wisconsin National Primate Research Center examined the effects of calorie restriction on 76 rhesus monkeys. The monkeys that had were fed low-calorie food (a 30% calorie reduction) looked much younger and showed a longer life span than control members (100% calorie) [47]. However, a similar experiment by the NIA in the United States in 2012 suggested the reverse results [48]. Therefore, further discussion is necessary to confirm the relationship between anti-aging and energy limitation.
\nResveratrol has been screened as a compound that is effective for enhancing the expression of the sirtuin gene [49]. When resveratrol was given to mice that had been fed a high-calorie diet, some of the factors of aging were inhibited, and the activities of AMPK and PGC-1α were enhanced; however, the anti-aging were insufficient. If compounds that can more effectively enhance the sirtuin gene are discovered, they will become a prominent anti-aging hormone.
\nPolyamines also show remarkable characteristics. Polyamines, such as spermine, spermidine, and putrescine, are essential for the growth of mammals and play important roles in cell division and differentiation; however, their precise action has not been sufficiently elucidated [50, 51]. In most cells of the human body, the concentrations are maintained at low levels. They are only found at high levels in cells that are actively working, such as testicular cells and cancer cells. The amounts of polyamines in those cells are especially increased before DNA synthesis; the growth rate in cancer cells increases in proportion to the amount of polyamine that is added to the medium. Polyamines are therefore related to the activation of cells.
\nSome studies suggest that polyamines function as an anti-aging hormone. The addition of polyamines to fibroblasts enhanced the expression of genes related to aging [20], and mice that were contentiously fed a diet containing a high concentration of polyamine appeared much younger and had a longer life span than mice that were fed a diet without polyamine [52]. Other researchers, who had studied the relationship between
In this chapter, the author focused on cellular aging and rejuvenation. Recent studies have gradually elucidated the aging process and have suggested that telomerase, the mitochondria and other components (RNAs and proteins) are involved in the aging process. However, these studies are ongoing. Moreover, studies on the aging process have identified several anti-aging compounds that can activate telomerase (TA-65), inactivate reactive oxygen species (anti-oxidants) and work as anti-aging hormones (klotho, sirtuin, resveratrol, and polyamine). In the near future, these compounds and/or other compounds with greater effects may become prominent drugs for anti-aging and rejuvenation.
\nResearch into regeneration with the use of iPS cells is recently showing remarkable progress. Although the topic was not introduced in this chapter, the regeneration of cutaneous and nerve tissues or internal organs using regeneration medicine will be another means of realizing rejuvenation. Researchers studying anti-aging will have to watch the progress of both regeneration medicine and cellular rejuvenation research.
\nDiapause is a state in which insects suspend or arrest the development in response to unfavorable environmental cues. It is an adaptive mechanism with a genetic basis, regulated by the external and internal environment factors, and occurs in a specific stage during the life cycle of an insect such as embryonic, larval, pupal, and active adult stage. Once induced, diapause cannot be immediately terminated even if unfavorable conditions disappear, unless a certain break period has been experienced. Diapause is terminated with the return of appropriate environmental conditions (temperature, light, moisture, etc.), and physical and chemical conditions [1]. Diapause provides an adaptive advantage for insects, allowing them to continue surviving in unfavorable seasonal environments and ensuring that their life cycle is synchronized with conditions suitable for growth, development, and reproduction.
There are two types of diapause, obligatory diapause and facultative diapause. Obligatory diapause, also known as absolute diapause, means that insects have to enter diapause to complete their life cycle, regardless of environmental conditions. It is most found in univoltine insects (one generation per year). For example,
Diptera is the fourth largest order after Coleoptera, Lepidoptera, and Hymenoptera. In terms of diapause research, 85 species of Diptera have been studied, including Tephritidae, Culicidae, Calliphoridae, Agromyzidae, Oestridae, Muscidae, Anthomyiidae, Tachinidae, Drosophilidae, and Cecidomyidae (Figure 1).
Taxa of dipause in Diptera.
Diapause can be categorized according to life stages as an egg (embryonic), larval, nymphal, or adult diapause (Table 1). Based on the hereditary feature, there are two types of diapause, one mandatory (obligate diapause) and one optional (facultative diapause) (Table 1).
Classification | Characteristics | |
---|---|---|
Diapause induction stage | Embryonic diapause | occurs at any stage of insect embryo development; regulated by the brain-hypopharyngeal gland-diapause hormone endocrine system |
Larval diapause | occurs at any instar of larval larvae, but mostly occurs at the late larval stage | |
Nymphal diapause | commonly happen in Diptera and Lepidoptera, which is regulated by brain-prothoracic-corpora allata interaction | |
Adult diapause | commonly occurs in Coleoptera, Lepidoptera, Hemiptera, Diptera, Homoptera, and Orthoptera, and is regulated by the islet- corpora allata | |
Hereditary feature | Obligatory diapause | insects have no choice but to enter this process at some stage in their life. |
Facultative diapause | insects will start this process only when environmental conditions become adverse |
Types of diapause.
Insects with obligate diapause enter diapause at a fixed developmental stage on schedule no matter how the external environmental conditions are, while those with facultative diapause can be induced into diapause at a certain stage but uncertain generation. Diapause is mainly affected by the changes in external environmental conditions. When the environmental conditions are unfavorable, insects enter the diapause, otherwise they continue to develop. Flies also experience diapause in the pupal stage (quiescent stage), during which the activity is extremely weak.
The diapause process can be divided into three phases: pre-diapause, diapause, and post-diapause. Pre-diapause occurs before the beginning of unfavorable environmental cues. Insects in this phase forecast an impending transformation in certain environmental stimuli in a special way, and change their internal neuroendocrine system and metabolism level to enter diapause. At this stage, insects maintain normal development, which include induction and preparation stage [14]. The induction stage happens before the beginning of unfavorable environmental cues. Insects receive these specific environmental stimuli called “token stimuli” such as photoperiod, temperature, humidity, and food, to regulate their development and decide whether to enter diapause or not. Larvae of
Because of frequent observations of changing responses to various environmental conditions, diapause is divided into three eco-physiological sub-phases: initiation, maintenance, and termination [14]. The incidence of diapause is affected by a variety of factors. Many insects enter diapause at any stage of their life cycle, but for some species, the diapause stage is fixed, which can be judged by observing the color, appearance, and cocoon making of insect bodies [14, 19, 20, 21]. The maintenance phase refers to the period in which the insect remains undeveloped even under favorable developmental conditions, and the respiration and metabolic rates are at low levels. The diapause maintenance period of different insects varies greatly, ranging from a few weeks to several months or even several years. With the return of favorable environmental conditions, the intensity of diapause gradually decreases and enters the diapause termination. At this stage, insects are sensitive to temperature factors, especially low temperatures. In addition, photoperiod can induce diapause termination. Application of exogenous substances can also break insect diapause. For example, exogenous ecdysterone can terminate the diapause of
In the termination phase, insects enter the next inactivity period if the environmental conditions are still unfavorable; however, they start resuming their physiological development [14].
Parents exhibit a greater effect on the diapause phenotype of their offspring, which defines as parental effect [23]. Parental effect is subject to natural selection, and it is the response mechanism of phenotypes to environmental heterogeneity [24, 25]. Among the parental effects, the female parent exhibits a greater influence than the male parent, so maternal inheritance is considered to be a short form of non-Mendelian parental effect (including maternal and paternal inheritance). For insects, female effects on offspring are relatively common. The parental effect of diapause in Diptera is mainly affected by environmental conditions such as diapause duration, photoperiod, temperature, and parental factors. Ordinarily, parents produce more diapausing progeny if they experience short-day length, limited resources, or low temperature [26]. In the study of
Larval diapause in the blow fly
Insect diapause is a complex process in which many mechanisms interact with each other. The major environmental signals regulating diapause onset in insects include temperature, photoperiod, food, and population density. Studies on insect diapause show that the primary cause and state of diapause can be identified and insects could be induced into diapause by artificially simulating diapause conditions in the field. Environmental cues, mostly temperature and photoperiod, control reproductive diapause in flies (Diptera), which regulate the induction, maintenance, and termination of diapause.
Seasonal change in photoperiod is the most reliable information to detect the time of year and is the major environmental signal regulating diapause onset in most insects. Photoperiod refers the period of time in a day that an organism is exposed to light or, more simply, day length. Photoperiodism is a biological response to a change in the proportions of light and dark in a daily (24 h) cycle, and the average number of daylight hours that cause insect diapause is known as “critical photoperiod.” When insects respond to changes in light intensity through the brain or compound eyes, the internal “timer” automatically evaluates the length of day or night and regulates the insects to enter the diapause [32]. Usually, the diapause of Diptera is caused by short-day length.
Temperature is another major environmental signal regulating diapause, especially for Diptera.
Diapause in some species of flies is subject to both photoperiod and temperature; for example, the dominant diapause cues of
Endogenous regulatory factors of insect diapause mainly focused on neuroendocrine systems, hormone signaling pathways, and energy metabolism pathways. Studies on
Juvenile hormone (JH), a sesquiterpenoid hormone produced by the corpus allatum (CA) of insects, is one of the most important hormones in insects and plays a key role in preventing larval metamorphosis, maintaining larval state, and regulating adult developmental and physiological process. It also plays a crucial role in the expression of vitellogenin, oocyte maturation, and development. The interaction between genes associated with juvenile hormone pathway is complex, which means genes directly or indirectly participate in the regulation of JH signaling pathway. Studies on the fly
During diapause, there are a lot of significant changes that occur in energy and metabolism due to the organism need to maintain life activities under extreme environmental conditions. Expression of the trehalase gene expression and enzyme activity of
With the development of high-throughput sequencing technology, the sequencing and annotation of
The use of RNA-Seq to determine genes with distinct levels of expression between diapause and non-diapause has been confined to flies, and Kyoto Encyclopedia of Genes (KEGG) analysis is performed to identify the pathways that are significantly enriched in diapause. A high-throughput RNA-Seq analysis from non-diapause and summer diapause pupae of
Based on transcriptome sequencing, some candidate diapause-related genes have been further studied in Diptera. For example, heat shock proteins (HSPs) have been studied in
Proteins are complex molecules that play a central role in biological processes. Proteomics is used to elucidate the expression and function of protein on the basis of genome research. Changes in protein expression during diapause can be explored by two-dimensional gel electrophoresis and mass spectrometry. Isobaric tag for relative and absolute quantitation (iTRAQ) can quantitatively analyze proteins from different sources in a single assay, and is used to study quantitative changes in the proteome by tandem mass spectrometry. Due to its high efficiency and sensitivity, iTRAQ has the potential to further advance the study of molecular mechanisms involved in diapause. Proteomic analysis of Diptera has also been reported. A proteomic approach was used to investigate the proteins extracted from larvae of
Metabolome refers to a collection of small molecular compounds that participate in the metabolism of an organism or cell with a relative molecular weight of less than 1000 DA in a specific physiological period. Metabolomics is a new discipline that simultaneously conducts qualitative and quantitative analysis of small molecule metabolite. It can be used to investigate how metabolites change with time when the organism is stimulated [66]. The cellular activities of living organisms are jointly undertaken by genes, proteins, and small molecule. The metabolic level can reflect the functional changes of macromolecules and amplify the small changes in gene expression. Various techniques are widely used to determine metabolic phenotypes, including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC/MS), and nuclear magnetic resonance (NMR). LC-MS analysis does not require sample volatileness and thermal stability, and is suitable for compounds with high boiling points, strong polarity, and poor thermal stability. Most metabolites involved in life science have these qualities, so LC-MS has broad application. In recent years, metabolomics has been applied in the area of medicine and microbiology, but there are still relatively few studies on insect diapause. The metabolic profiles of diapause and non-diapause
Knowledge of diapause in Diptera is essential for the development of effective pest management strategies and to increase the shelf-life of parasitoids used in the biological control industry. This chapter summarizes the recent progress on diapause of Diptera. We do believe that further studies should be investigated in the diapause of Diptera. Current research studies suggest that histone modification, DNA methylation, RNA methylation, and small noncoding RNAs all may be involved in the regulation of diapause in Diptera. However, it remains unclear whether they regulate the hormonal and physiological changes associated with diapause of Diptera; research has primarily focused on physiological changes associated with pre-diapause with limited attention given to post-diapause. Studies showed that the indices of insects, such as oviposition quantity, oviposition duration, and life span, increased positively during post-diapause phase. The accumulation and consumption of energy storage substances in pre-diapause and diapause will affect the biological characteristics in post-diapause phase. Combined with biological characteristics in post-diapause, studies on development rate, feeding, individual size, diapause maintain environmental conditions, and nutritional supplements after the diapause are necessary. Existing studies on diapause in Diptera are mainly based on single omics, and studies using multi-omics are still vacant. Therefore, for a deeper understanding of the complex molecular landscape of diapause in Diptera, all the available omics data should be utilized in combination rather than treating them individually.
This study was supported by the Science and technology planning project of Inner Mongolia Autonomous Region (2021GG0057, 2020ZY0001, 2019BS03011).
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Subtle changes that occur over time in periodontal tissues that are below the detection limit of visual examination or periodontal probing can be found and tracked accurately over time using 3D imaging, fluorescence spectroscopy, and optical coherence tomography. During debridement of teeth and dental implants, the effective removal of subgingival microbial biofilms and dental calculus deposits can be enhanced using magnifying loupes and operating microscopes and by novel methods based on the interactions of light with bacterial deposits, such as differential reflectometry and light-induced fluorescence. While such techniques can also be used using initial case assessment, their primary purpose is for checking debridement procedures, since the point when bacterial deposits are no longer present represents an endpoint for treatment. The concept of real-time feedback has been developed, using fluorescence readings to control the removal of deposits. 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He has been reviewer for several publications of the Optical Society of America\\'s including Photonics Technology Letters and Applied Optics.\n\nPersonal Interests\nThese include motor cycling in a very relaxed manner and performing martial arts.",institutionString:null,institution:{name:"Charité",country:{name:"Germany"}}},{id:"341622",title:"Ph.D.",name:"Eduardo",middleName:null,surname:"Rojas Alvarez",slug:"eduardo-rojas-alvarez",fullName:"Eduardo Rojas Alvarez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/341622/images/15892_n.jpg",biography:null,institutionString:null,institution:{name:"University of Cuenca",country:{name:"Ecuador"}}},{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/215610/images/system/215610.jpeg",biography:"Muhammad Sarfraz is a professor in the Department of Information Science, Kuwait University. His research interests include computer graphics, computer vision, image processing, machine learning, pattern recognition, soft computing, data science, intelligent systems, information technology, and information systems. Prof. Sarfraz has been a keynote/invited speaker on various platforms around the globe. He has advised various students for their MSc and Ph.D. theses. He has published more than 400 publications as books, journal articles, and conference papers. He is a member of various professional societies and a chair and member of the International Advisory Committees and Organizing Committees of various international conferences. Prof. Sarfraz is also an editor-in-chief and editor of various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/267434/images/system/267434.jpg",biography:"Dr. Rohit Raja received Ph.D. in Computer Science and Engineering from Dr. CVRAMAN University in 2016. His main research interest includes Face recognition and Identification, Digital Image Processing, Signal Processing, and Networking. Presently he is working as Associate Professor in IT Department, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur (CG), India. He has authored several Journal and Conference Papers. He has good Academics & Research experience in various areas of CSE and IT. He has filed and successfully published 27 Patents. He has received many time invitations to be a Guest at IEEE Conferences. He has published 100 research papers in various International/National Journals (including IEEE, Springer, etc.) and Proceedings of the reputed International/ National Conferences (including Springer and IEEE). He has been nominated to the board of editors/reviewers of many peer-reviewed and refereed Journals (including IEEE, Springer).",institutionString:"Guru Ghasidas Vishwavidyalaya",institution:{name:"Guru Ghasidas Vishwavidyalaya",country:{name:"India"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:null,institution:{name:"Beijing University of Technology",country:{name:"China"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"265335",title:"Mr.",name:"Stefan",middleName:"Radnev",surname:"Stefanov",slug:"stefan-stefanov",fullName:"Stefan Stefanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/265335/images/7562_n.jpg",biography:null,institutionString:null,institution:{name:"Medical University Plovdiv",country:{name:"Bulgaria"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Igor Victorovich Lakhno was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPh.D. – 1999, Kharkiv National Medical Univesity.\nDSC – 2019, PL Shupik National Academy of Postgraduate Education \nProfessor – 2021, Department of Obstetrics and Gynecology of VN Karazin Kharkiv National University\nHead of Department – 2021, Department of Perinatology, Obstetrics and gynecology of Kharkiv Medical Academy of Postgraduate Education\nIgor Lakhno has been graduated from international training courses on reproductive medicine and family planning held at Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor in the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics, and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s been a professor in the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics, and gynecology department. He’s affiliated with Kharkiv Medical Academy of Postgraduate Education as a Head of Department from November 2021. Igor Lakhno has participated in several international projects on fetal non-invasive electrocardiography (with Dr. J. A. Behar (Technion), Prof. D. Hoyer (Jena University), and José Alejandro Díaz Méndez (National Institute of Astrophysics, Optics, and Electronics, Mexico). He’s an author of about 200 printed works and there are 31 of them in Scopus or Web of Science databases. Igor Lakhno is a member of the Editorial Board of Reproductive Health of Woman, Emergency Medicine, and Technology Transfer Innovative Solutions in Medicine (Estonia). He is a medical Editor of “Z turbotoyu pro zhinku”. Igor Lakhno is a reviewer of the Journal of Obstetrics and Gynaecology (Taylor and Francis), British Journal of Obstetrics and Gynecology (Wiley), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for a DSc degree “Pre-eclampsia: prediction, prevention, and treatment”. Three years ago Igor Lakhno has participated in a training course on innovative technologies in medical education at Lublin Medical University (Poland). Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: are obstetrics, women’s health, fetal medicine, and cardiovascular medicine. \nIgor Lakhno is a consultant at Kharkiv municipal perinatal center. He’s graduated from training courses on endoscopy in gynecology. He has 28 years of practical experience in the field.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. 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The global picture of physiological processes in plants needs to be investigated continually to increase our knowledge, and the resulting technologies will benefit sustainable agriculture.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/13.jpg",keywords:"Plant Nutrition, Plant Hormone, Photosynthesis, Respiration, Plant Stress, Multi-omics, High-throughput Technology, Genome Editing"}],annualVolumeBook:{},thematicCollection:[],selectedSeries:null,selectedSubseries:null},seriesLanding:{item:{id:"6",title:"Infectious Diseases",doi:"10.5772/intechopen.71852",issn:"2631-6188",scope:"This series will provide a comprehensive overview of recent research trends in various Infectious Diseases (as per the most recent Baltimore classification). Topics will include general overviews of infections, immunopathology, diagnosis, treatment, epidemiology, etiology, and current clinical recommendations for managing infectious diseases. Ongoing issues, recent advances, and future diagnostic approaches and therapeutic strategies will also be discussed. This book series will focus on various aspects and properties of infectious diseases whose deep understanding is essential for safeguarding the human race from losing resources and economies due to pathogens.",coverUrl:"https://cdn.intechopen.com/series/covers/6.jpg",latestPublicationDate:"August 18th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:4,numberOfPublishedChapters:126,numberOfPublishedBooks:13,editor:{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null},subseries:[{id:"3",title:"Bacterial Infectious Diseases",keywords:"Antibiotics, Biofilm, Antibiotic Resistance, Host-microbiota Relationship, Treatment, Diagnostic Tools",scope:"