\r\n\tBesides the development of cryptographic tools (hardware and software), we are also interested in the applications of cryptography in current and future scenarios like blockchain, internet of things, industry 4.0, privacy enforcement, cybersecurity and special algorithms such as zero-knowledge proofs and identity-based cryptography.
\r\n\r\n\t
\r\n\tFinally, cryptography does not live in a vacuum, but it is intertwined with our society, sometimes rising questions that are beyond the purely technical realm. For example: Is the weakening introduced by key escrow mechanisms worth the gain? How does cryptography interact with privacy rights? What is the economic impact of cryptography? We welcome contribution to those less technical questions as well.
Nature Aided Drug Discovery (NADD) represents the most ancient approach in finding new active compounds for fighting human diseases, and still today it plays a crucial role in drug discovery [1]. New chemical entities (NCE) from natural derivation represent a relevant slice among the drugs approved by Food and Drug Administration (FDA) and the European Medicines Agencies (EMA) for commercialization and administration on humans [2]. More than half the total anti-infective drugs approved in the last forty years resulted from a NADD approach and a similar trend can be observed for anticancer drugs, where 41% of them derived from natural sources and only 16% are classifiable as totally synthetic small molecules [2]. Moreover, the Global Herbal Medicine Market Size is expected to increase to USD 129 billion by 2023, according to Market Research Future [3].
The success of NADD finds its main reason in the wider and heterogeneous chemical space covered by natural products whether compared with synthetic derivatives. The 83% of the chemical scaffolds found in natural compounds are unique and absent in synthetic NCE, due to the lack of commercially available synthons or cumbersome and prohibitive synthetic procedures [4]. Thus, the screening of libraries of compounds derived from natural sources still remains a worthy procedure for the identification of new and unexplored NCE. Besides, marine sources or lichens are still almost uninvestigated and might therefore represent an inestimable treasure of new potential drugs [5, 6, 7].
The natural compounds of interest in NADD are secondary metabolites that are not directly involved in the essential functions of the cell cycle and duplication processes and are characterized by high structural variability. From a structural standpoint, they are classified into alkaloids, terpenoids, saponins, lignans, flavonoids, and tannins [8]. Secondary metabolites are produced in different amounts for vexillary functions or defensive responses to biotic or abiotic stress being involved in the system of plant defense [9]. For this reason, specific secondary metabolites may be considered as markers of the plant health and may be used to evaluate the quality of the selected natural matrix and the effects that the environmental factors have on it. The evaluation of their content in natural sources, as well as their recovery, require the exploitation of ad hoc extractive procedures since secondary metabolites are mainly present in the intracellular domain. Thus, appropriate extraction procedures involve the rupture of the cell wall and cellular membranes, thus favoring the passage of the secondary metabolites into the extraction solvent, from which they can be recovered.
Over the years different protocols and techniques have been developed for the extraction of metabolites from natural matrices, named Solid–Liquid Extraction (SLEs). SLEs are classified in conventional (or traditional) and non-conventional (or innovative) methodologies [10]. In conventional methodologies, SLE is performed by heating the natural source with conventional heating sources (i.e., flame, heating plate, or mantle) in the presence or not of solvent and with or without stirring. Examples of conventional methodologies are maceration (digestion, infusion, and decoction), percolation, and Soxhlet or steam distillation. Solvents, or generally mixtures of solvents, with wide grades of polarity such as methanol, ethanol, acetone, ethyl acetate, trichloromethane, hexane,
In this chapter, the potential of microwave-assisted irradiation for the extraction of secondary metabolites from plants, and natural resources in general, will be discussed, with a special focus on recent applicative examples of the most representative chemical classes.
The physical principles underlying MASE are completely different from those of conventional extraction because microwave irradiation can cause a more effective disruption of the cellular structures (cell walls and cellular membranes) thus favoring the release of the cellular content and speeding up the extraction process. The interaction of microwave irradiation with the solvents, intracellular water, and ions causes an increase of the dipolar rotation and ionic conductivity of molecules with dipolar moments and ions, which results in a rise of the temperature inside the cell. The vaporization of the intracellular water causes the dehydration of the cellular wall and the reduction of its resistance. This, combined with the abrupt increment of the intracellular pressure, leads to the cell wall and membranes disruption, thus facilitating the passage of the secondary metabolites into the extractive solvent [12, 13].
This is the result of a synergistic combination of heat and mass gradient working in the same direction from the inside to the outside of the cell, as confirmed by Scanning Electron Microscopy (SEM) analysis (Figure 1) [14].
Microwave heating effect on cells.
Monomodal and multimodal microwave systems also referred to as single- or multi-mode systems specifically designed for MASE are nowadays available. A single-mode system permits to focus the microwave radiation on a restricted zone where the sample is subjected to a much stronger electric field. Conversely, in a multimode system, the microwave radiation is randomly dispersed within the microwave cavity, where the sample is irradiated [15]. Both mono- and multi-modal microwave devices comprise a magnetron, which generates microwave radiation, a waveguide, which is used to propagate the microwave from the source to the microwave cavity, the applicator, where the sample is placed and a circulator that allows the microwave to move only in the forward direction. The main difference between the two systems relies on the applicator that in the case of a multimodal system is a closed cavity where the microwaves are randomly dispersed, whereas in the monomodal oven the extraction vessel is directly located in-line with the waveguide [15]. Both systems are effective in extracting metabolites from natural sources, and their use is usually related to the amount of natural matrix. Accordingly, the monomodal system is preferred when the amount of natural source to be treated is relatively low (milligram to gram scale), and vice versa. Microwave extraction may be performed using open or closed extraction vessel systems. The open vessel apparatus originated from a modification of domestic MW ovens. The extraction is conducted at ambient pressure and the vessel is directly connected to a condenser to avoid loss of solvent or volatile components. An evolution of the open vessel apparatus is the Focused Microwave-Assisted Soxhlet (FMASE) which combines the classical Soxhlet extraction (SE) technique with MW irradiation. In the closed vessel system the entire extraction vessel is located within the oven, thus allowing better control of the pressure and temperature during the extraction [16].
The closed-vessel system presents several advantages: i) a higher temperature than open vessel systems can be reached because of the increased pressure inside the vessel raises the boiling point of the solvents used, thus decreasing the time needed for the extraction; ii) the loss of volatile substances is completely avoided because they are confined within the vessel; iii) a low amount of solvent is required because no evaporation occurs and there is little or no risk of airborne contamination thus preventing the oxidation of air-sensitive metabolites; iv) the fumes are contained within the vessel, reducing the hazard of the extractive procedure.
In a closed vessel system high pressure can be reached. The high pressure stimulates various phenomena according to LeChatelier’s principle, such as the transition of phase from one form to another, change in reaction dynamics, change in molecular structure,
Nevertheless, the use of closed-vessel systems presents some drawbacks: the amount of samples that can be processed is limited; the addition of reagents or solvents during operation is forbidden and the vessel must be cooled down before it can be opened to avoid loss of volatile constituents [16]. For processing a large amount of material, an open-vessel system is more appropriate. It allows the possibility to add reagents and to refill the solvent during the extraction, and to remove the excess of solvent during the extraction procedure. Moreover, the extraction of thermolabile metabolites is allowed since it usually reaches low temperatures relative to closed-vessel systems. On the other hand, the open-vessel systems ensure less reproducible results compared to the closed-vessel systems and the extraction in open-vessel could require a longer time to obtain results comparable to those achieved in closed-vessel [16].
The microwave energy may be applied also to extraction in solvent-free conditions (Solvent Free Microwave Extraction-SFME) [12]. In this case, the plant material is directly placed into the microwave reactor, without the addition of any solvent. The heating of the water contained by the plant material distends the natural matrices and causes the rupture of the cells releasing their content. This process has been successfully applied for the extraction of the essential oils. After MW heating, the volatile components co-evaporate with the in-situ water contained in the natural matrix; the vapors condense outside the microwave oven into a collector where they separate between essential oils and the aqueous phase. The latter is brought back into the vessel to refresh the amount of water in the sample, whereas the essential oil is collected apart [12].
The performance of the MASE process is strictly related to the operative conditions employed. Several parameters, such as solvent, solvent-drug ratio, temperature, time, pressure, microwave power, water content, and characteristic of the matrix must be optimized in setting up a MASE procedure. Each of these parameters should not be considered alone, but they are all linked together and the comprehension of the effects and influences of these factors is pivotal for MASE efficiency. A brief discussion about the role of these parameters in the design of a MASE protocol is herein reported.
The selection of the solvent plays a crucial role in MASE, as well as in other conventional extraction processes, and several solvent parameters (solubility of the analyte, penetration, interaction with the matrix, dielectric constant, and mass transfer kinetic process) should be considered to perform the right choice.
As stated in the previous paragraph, a Solvent-Free Microwave Extraction (SFME) is also possible. This procedure foresees the direct irradiation with MW of the plant material, fresh or rehydrated. A crucial role is played by the content of water in the sample, because it is the heating of such water to cause the rupture of the cells of the plant material, favoring the release of the content. This process is mainly applied for the extraction of volatile compounds such as essential oil. The oil evaporates by azeotropic distillation with the water contained in the sample. After cooling outside of the microwave reactor the oil separates from the water and can be collected through a modified Clevenger apparatus. Water is refluxed back into the microwave oven to allow the continuous extraction of the oil from the sample [12].
Another important parameter to consider in the set-up of a MASE protocol is the ratio between the amount of sample and the volume of solvent. The latter should be enough to cover the sample during the entire process, especially when the matrix swells during the extraction process. Although in conventional extraction higher is the volume of solvent, higher is the yield of the extract, in MASE larger volume of solvent may result in more energy and time to heat the suspension and in a lower yield due to a non-uniform distribution and exposure to microwave. Usually, an L/S from 10:1 to 20:1 (mL/mg) is found to be the right ratio in many extractive processes reported in the literature [20].
Strictly correlated to the L/S ratio is the stirring rate since it affects the mass transfer process in MASE. However, the significance of this parameter is rarely explored. By stirring, the mass transfer barrier created by the concentrated compounds in a localized region due to insufficient solvent can also be minimized resulting in better extraction yield. In other words, agitation accelerates the extraction speed by accelerating the desorption and dissolution of compounds bound to the sample matrix [17].
Extraction times in MASE lasts from a few minutes up to half an hour, and this represents an advantage for the extraction of thermal or oxygen labile compounds since it avoids the degradation of the compounds of interest [21]. The extraction yield is directly proportional to the extraction time, although it has been observed that this increment is very small for an extremely long time. Moreover, for longer extraction time overheating may occur, especially with high absorbent solvents, thus exposing thermolabile compounds to degradation. Whether longer extraction time is required, consecutive and shorter extraction cycles are preferable. The solvent can be collected after each extraction cycle and a fresh solvent could be added to the residue to guarantee the exhaustion of the matrix. This discontinuous procedure has been applied for the extraction of several secondary metabolites from plant material, allowing an enhanced yield and low decomposition of thermolabile compounds [22].
Temperature and MW power are strictly correlated. Power is the driving force of the process since it provides the energy necessary to excite the dipolar moments and the ionic conduction of the constituent of the sample, resulting in a proportional increase of the temperature and promoting the destruction of the natural matrix. Thus, the power of the microwave irradiation has to be carefully dosed in function of the amount of the sample, the solvent employed, the extraction time required, and the chemical stability of the secondary metabolites of interest [23]. Increasing the power results in an improved extraction yield and shorter extraction time. However, this result is true until the reaching of an optimal temperature beyond which a decrease in yield is observed, mainly due to the thermal stability of the target metabolite [24].
Accordingly, the temperature is a key parameter to enhance the efficacy of MASE and to avoid at the same time the degradation of the sample. Thus, the choice of the extraction temperature is strictly related to the properties of the solvent, the chemical stability of the metabolites of interest, and the microwave system used. In particular, at high temperatures the viscosity and the surface tension of the solvent diminish; moreover, the capability to solubilize the analytes, and to wet and penetrate the matrix increase, thus resulting in improved extractive efficacy. Also, when operating in a close-vessel, there is the advantage of heating the solvent above its boiling point, thus leading to a more performing extraction [25].
To conclude, the development of a proper MASE methodology must consider at least four variables: solvent, liquid/solid ratio, temperature, and time. To speed up the set-up of the procedure, the Design of Experiment (DoE) approach may be applied. This is a systematic statistic-based tool to assess the best experimental conditions both in the academic and industrial fields [26]. Thanks to this approach, all the variables and their interactions can be evaluated while doing the minimum number of runs.
Over the years, microwave-assisted extraction has been successfully applied to extract diverse classes of secondary metabolites (i.e. polyphenols, flavonoids, coumarins, terpenoids, cannabinoids, and alkaloids) from natural sources, for evaluating the plant productivity, for extracting bioactive compounds both for drug discovery or for commercial purposes.
Here below, studies of plant productivity based on MASE methodology and specific cases of extraction of natural compounds of pharmaceutical and nutraceutical interest will be discussed, with a special focus on resveratrol, terpenoids, and cannabinoids.
Numerous applications report about the use of the MW to assist the extraction of organic and organometallic compounds from various matrices (soils, sediments, water samples, botanicals), with special emphasis on environmental applications [27, 28]. Extraction of natural matrices is essential to compare their productivity under different stress conditions [9], harvesting time [29], and places [30]. MASE offers the possibility of performing multiple extractions and therefore, it is suitable for the rapid screening of a numerous set of samples to evaluate the productivity of organisms.
An example is a work performed by Martino et al., regarding the MASE of
Secondary metabolites extracted via MASE approach and considered as markers of the plant productivity.
Another example is the setup of a fast and reproducible extraction methodology of vitexin and its isomer isovitexin from
Within this context, Martino et al. set up a MASE procedure that can be applied for quantitative extraction of both metabolites from
MASE can also be applied to study the effect of micronutrients or pollutants on secondary metabolites production. Amri et al. investigated the impact of soil copper (II) concentrations on nutrient uptake and the antioxidant system of
To sum up, MASE procedures is a versatile technique suitable for the evaluation of the plant productivity, and to assess the quality of vegetal matrices, since it is fast, reproducible, suitable for extraction of a large number of samples and requires a low amount of natural matrix.
Alkaloids are a well-known class of secondary metabolites characterized by basic nitrogen. Over the years, many active alkaloids have been extracted via MW irradiation, e.g. ephedrine alkaloids, cocaine, and ergot alkaloids [35, 36, 37]. Unfortunately, results obtained for many of them have been comparable or worst if compared with the traditional method [38]. Nevertheless, microwaves have also spurred the discovery of new active alkaloids at the early stage of drug discovery. MASE protocols can be exploited to extract different alkaloids (examples are reported in Figure 3) from different botanicals like tuberous roots, leaves, and seeds [37, 39, 40].
Examples of alkaloids extracted via the MASE approaches.
As an example of MASE applied to the extraction of alkaloids, Pan et al. obtained a good recovery of caffeine and polyphenols from the leaves of green tea (
Xiong et al. developed an efficient MASE protocol, within a drug discovery process, for the isolation of bioactive alkaloids (e.g. liensinine, isoliensinine, neferine, dauricine, nuciferine, Figure 3) from
Recently, Belwal et al. reported an optimized MASE protocol, defined by multicomponent analysis, for the extraction of berberine (Figure 3) and polyphenols from diverse species of
Regarding the alkaloids employed as drugs, or of interest for the toxicological use and/or abuse, few extractive procedures by MASE are reported in the literature. As an example, Brachet et al. extracted cocaine and benzoylecgonine from the leaves of
Stilbene-based polyphenolic compounds, i.e. resveratrol, pterostilbene, and piceatannol, are of particular interest from a medicinal chemistry standpoint, having multiple pharmacological activities (Figure 4).
Chemical structure of polyphenolic-stilbene based secondary metabolites.
In particular,
Even though resveratrol is produced naturally in plants, the extraction of resveratrol in commercial quantities is a problem, because of its low concentration, multiple steps of isolation and purification, unfriendly environmental issues, and seasonal occurrence [53]. Moreover, the preparation of resveratrol by synthesis is difficult owing to the formation of many unwanted side products [54, 55]. Only recently, the production of resveratrol in heterologous engineered microorganisms has been proposed [56]. Thus, resveratrol is still being extracted from wild
The extraction of resveratrol by MASE from different plant materials (i.e.
In another study, Dang et al. combined the aqueous two-phase extraction technique (ATPE) with MASE for the extraction of the total polyphenol content, including resveratrol, from grape seeds [60]. Microwave-assisted ATPE (MAATPE) required lower solvent concentration and less time compared with other methods such as refluxing solvent or SE. A higher level of resveratrol was obtained with MAATPE, in contrast to ATPE. The Authors also compared the effectiveness of three solvents (water, water: ethanol (1:1) and ethanol) and three extraction methods, including MASE and ultrasound-assisted extraction (UAE) and the conventional SE. MASE provided a better extraction with water and ethanol (1:1) obtaining extracts very rich in polyphenolic substances, including stilbenes.
Lastly, MASE has successfully applied also for the extraction of other polyphenolic-stilbene based compounds such as pterostilbene, mainly found in blueberries and in
Terpenes and isoprenoids, in general, gained much attention for their physiological functions (i.e., hormones, aliphatic membrane anchors, maintaining membrane structure), ecological roles (i.e., defense compounds, insect/animal attractants), and extensive pharmaceutical applications such as flavors, fragrances, and medicines.
In particular, artemisinin and paclitaxel represented two milestones in the fight against malaria and cancer, respectively. Artemisinin (Figure 5) is a sesquiterpene lactone isolated from
Chemical structures of artemisinin and paclitaxel.
Recently, Misra et al. developed a rapid and reliable MASE and HPTLC protocol for the analysis of artemisinin. The optimized MASE conditions required 100 mg of dried and grinded drug with a size of 14 mesh dispersed into 10 mL of toluene. The irradiation of the sample at 160 W for 120 seconds led to the extraction of 0.816% of the content of artemisinin.
Paclitaxel (Figure 5) is a member of the taxane class, and it is one of the most important anticancer drugs approved for human use against ovarian, breast, and pulmonary cancer.
Although the total synthesis of paclitaxel has been reported, its application for the commercial production of this drug is impracticable. Thus, paclitaxel is still produced by extraction from taxol biomass. The most commonly used methods for the extraction of paclitaxel require the use of methanol at ambient temperature, although other protocols requiring refluxing methanol, 1:1 methanol-chloroform at ambient temperature, and percolation using ethanol or 95% ethanol-water at ambient temperature have been reported. However, these methods require a long time (12–24 h) for a complete extraction. Incorvia-Mattina et al. reported for the first time in 1997 the use of MASE to optimize the efficiency of the extraction of paclitaxel. The effects of the biomass, solvent ratio and water content on taxane recovery were also determined. Under appropriate MASE conditions an extract equivalent to the one obtained by conventional extraction methods was produced [63].
Talebi et al. investigated the use of MASE to extract paclitaxel from the needles of
Recently, another study for the extraction of paclitaxel from biomass through MASE and based on kinetic and thermodynamic analysis has been carried out. The majority of paclitaxel was recovered from the biomass (∼99%) within 6 min in a single cycle of microwave-assisted extraction at microwave powers of 50–150 W and temperatures of 30–45°C [64].
Chemical structures of CBD-like and THC-like major phytocannabinoids present in
CBD-like derivatives are non-psychotropic compounds but with other recognized pharmacological properties such as anti-inflammatory, antioxidant, and anticonvulsant. As an example, Epidiolex, a CBD-based anticonvulsant drug, has been approved in 2018 by Food and Drug Administration for the treatment of seizures associated with Lennox–Gastaut syndrome (LGS), Dravet syndrome, or tuberous sclerosis complex (TSC) in patients 1 year of age and older.
The discovery of a plethora of pharmacological activities ascribed to CBD and other minor phytocannabinoids has increased attention from both scientists and industries for medical, nutraceutical, and cosmetic applications of these cannabinoids.
Several synthetic procedures have been developed and optimized for the industrial preparations of phytocannabinoids and in particular of CBD. However, this process suffers from several drawbacks such as the cost of the starting materials, reagents and solvents, the formation of by-products with consequent cumbersome purification procedures, and the difficulty to control the stereochemistry, the isomerism of the terpenic double bond, and the easy interconversion of CDB into THCs in the synthetic conditions.
Thus, the extraction and purification of phytocannabinoids from
Focusing on MASE, Lewis-Bakekr et al. investigated the potential to directly extract and decarboxylate dried Cannabis material with the microwave reactor [69]. Dried plant material, suspended in ethanol, was subjected to heating with stirring in a microwave reactor at 150° C. Extraction yield for the concentrated resin was in the range of 19.6–24.4% and it resulted directly proportional to the heating time and dependent on the cultivar employed in the process. Interestingly, a complete decarboxylation of the phytocannabinoids was achieved in one step following this process and no acid forms of phytocannabinoids such as Δ9-THCA and CBDA were detected in the resulting extract. Thus, MASE proves to be a worthy method for extraction and decarboxylation of phytocannabinoids due to the possibility to apply controlled temperatures and shorter extraction times. Moreover, this procedure ensures a more consistent and reproducible
The effect of time and temperature was investigated first. Extraction and decarboxylation of phytocannabinoid resulted in time and temperature dependence. To obtain 100% decarboxylation, the temperature must be sustained over a period without the burning of the cannabis material or the boiling/evaporation of the solvent. Because the solvent of choice is ethanol (b.p. 78°C at 1 atm), to reach a higher boiling temperature (i.e. 100–170°C) the extraction process must be carried out in a sealed vessel and under pressure. 170°C was the highest operative temperature achieved since higher temperatures (>180°C) resulted in the microwave run abortion due to the high pressure reached within the vial.
The extraction of cannabis by MASE at 100°C, 130°C, 150°C, and 170°C for 10 minutes resulted in a 23–25% yield of extract.
Interestingly, it appeared that the addition of a second step, such as SFE, after the MASE did not change the cannabinoid profile in the extract. Thus, MASE alone can perform an almost complete extraction of the cannabinoids from the cannabis plant material. Besides, the extraction and conversion of THCA and CBDA into THC and CBD was better at a temperature above 130°C, than at 100°C.
MASE was compared with the effectiveness of the commonly employed extractive procedure, namely maceration in ethanol, SE, and SFE. The conventional extractive procedures resulted in a low concentration of Δ9-THC, THCA, and CBD, whereas the addition of the microwave step resulted in a significant increase in the concentration of CBD and THC. As expected, no THCA was detected.
To sum up, a worth general procedure for the extraction and decarboxylation of CBD and THC from cannabis plant material can be thus resumed: i) the drug is weighed and macerated in a mortar; ii) the grinded drug is charged in a microwave vial along with a stir bar; iii) the drug is submerged with ethanol and the vial is sealed; iv) the vial is irradiated with MW using the following conditions [a) Pre-stirring = 30 sec; b) run time = 10 min; c) temperature = 150°C; d) absorption = Normal]; v) the suspension is filtered, and the filtrate concentrated; iv) residual plant material may be subjected (but not necessarily) to SFE.
Drinic et al. extended these studies over other polyphenols and flavonoids as well as phytocannabinoids [71]. In particular, the effects of different extraction parameters, namely ethanol concentration, extraction time and solid/liquid ratio on extraction yield, total phenol content, total flavonoid content, antioxidant activity, reductive capacity, CBD content, and THC content were investigated. For MASE, a domestic microwave oven and a round-bottom flask connected with a condenser were used. The solid drug was mixed with the solvent (30, 50, or 70% v/v ethanol) in the selected solid/liquid ratio (S/L = 5, 10, or 15). The extraction was performed irradiating at a potency of 580 W without agitation and for a total extraction time of 10, 20, or 30 min. The results of each extraction were analyzed using response surface methodology. The influence of the three process parameters was investigated on total polyphenols yield, total flavonoids yield, antioxidant activity, and reductive capacity as well. The optimal conditions for the highest CBD content and lowest THC content resulted in 47% ethanol concentration, 10 minutes of extraction time, and an S/L ratio of 5. The model was successfully validated by preparing the Cannabis extract under the calculated conditions.
Alongside the pharmaceutical uses of Cannabis extracts, hemp seeds are widely employed to produce hemp oil. However, the content of Δ9-THC in the processed hemp seed oils must be under the limits imposed by the jurisdictions of each State. Indeed, although the hemp seeds produce negligible amounts of THC, their outer surface can be contaminated with the enriched in the phytocannabinoids resin secreted by the seeds’ bracts. The presence of Δ9-THC in the final hemp seed products had led to intoxication symptoms in the final consumers. Thus, nowadays the content of THC in hemp products is tightly regulated. Yang et al. investigated the effectiveness of various chemical procedures for the extraction of Δ9-THC from three brands of hemp seeds and how the extractive methods could influence their commercialization [72]. Four extraction methods were employed, namely, i) microwave extraction, ii) sonication, iii) SE and iv) SFE. As already investigated by Kore et al., the extraction was performed in ethanol at 150°C with stirring, obtaining a complete conversion of CBDA and THCA into the corresponding neutral form. Hemp seeds were macerated in a mortar, transferred into a microwave vessel, and suspended in ethanol. The suspension was irradiated at 150°C with stirring for 20 min in a closed vessel. The yield of the resin (27–38%) achieved was comparable to the other three extraction procedures. In contrast, SE provided higher yields of Δ9-THC and CBD than the other procedures, resulting in a more robust and appropriate extraction methodology for the testing of hemp seed products. Since the same solvent was used in all the compared extractions, the differences in the number of phytocannabinoids can be attributed to the extraction methods themselves. The results suggest that prolonged heating and solvent cycling in extracting phytocannabinoids from lipid-rich materials such as hemp seeds is mandatory.
MASE has rapidly risen during the latest decades as a method for the extraction of secondary metabolites or compounds of pharmaceutical and nutraceutical interest. The use of microwave can generate peculiar, and otherwise impossible to reach extraction mechanisms. As a result, a reduction of the extraction time, improvement of the extraction efficiency, high reproducibility, and robustness of the procedure can be achieved. An increase of the sample throughput is in addition possible, thus it can be considered as the elective technique when a high number of samples have to be processed specially during the first stage of the NADD process, and for evaluating the quality of the natural matrices [9, 29]. For these reasons, MASE has proven to be effective in all aspects, including economical and practical, compared to traditional extraction techniques, especially over SE. Conversely, in MASE the development of the method must be carefully assessed, and all the variables and factors described above must be thoroughly considered to provide some extraction selectivity. Hence, DoE, response surface methodology, and other statistical approaches are of great help to quickly determine the best conditions to achieve the highest yield of the metabolite of interest from the natural source. However, in the past year, the application of MASE in scalable industrial processes has always encountered several limitation due to the presence of some technological barriers, mainly related to the design of safe instrumentation. Thanks to the technological progress witnessed in recent years, the first industrial-scale ovens finally became commercially available [73, 74].
Food, pharmaceutical, and nutraceutical industries would be benefited from this emerging technology of MASE, which is an excellent substitute for traditional methods such as SE, and other environmentally benign technologies. The promise to be the technique that can respond to the necessities in this field will make MASE the extraction method of choice for the next years.
The authors declare no conflict of interest.
IntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
",metaTitle:"Retraction and Correction Policy",metaDescription:"Retraction and Correction Policy",metaKeywords:null,canonicalURL:"/page/retraction-and-correction-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
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\\n\\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
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\\n"}]'},components:[{type:"htmlEditorComponent",content:'IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n1. RETRACTIONS
\n\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\nPublishing of a Retraction Notice will adhere to the following guidelines:
\n\n1.2. REMOVALS AND CANCELLATIONS
\n\n2. STATEMENTS OF CONCERN
\n\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n3. CORRECTIONS
\n\nA Correction will be issued by the Academic Editor when:
\n\n3.1. ERRATUM
\n\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n3.2. CORRIGENDUM
\n\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n4. FINAL REMARKS
\n\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\n\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\n\nPolicy last updated: 2017-09-11
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