Assessment of plaque accumulation by a modified Plaque Index [11].
\r\n\tDigital images can be easily distorted by noise during the acquisition, processing, and transmission. Noise level is an important parameter to consider in image processing algorithms, including denoising, compression, feature extraction, motion estimation, optical flow, segmentation, super-resolution, and image quality assessment. Their performance depends on the accuracy of the noise level estimate.
\r\n\r\n\tImage denoising is an important stage to improve the accuracy of many image processing techniques, such as image segmentation and recognition. Image segmentation is another important stage in computer vision applications. Many methodologies utilize both stages in a unique algorithm to solve the problem of the segmentation of noisy images to provide better classification and recognition compared to algorithms that independently use these two stages.
\r\n\tThe goal of this book will be to collect original research chapters that develop or apply new theories and/or hardware or software to process the acquired noisy images to solve the problem of Segmentation of noisy images in the field of medical imaging, remote sensing, engineering, and other research applications.
Adequate supportive periodontal treatment visits are needed for the long- term success of implants [1]. Some percentage of implants ultimately fail and majority that fail, do so soon after placement [2]. Early indicators of future implant failure includes excessive plaque accumulation, Bleeding on probing, Increased probing depth, suppuration, radiographic bone loss, retrograde wear and broken restorations and hence supportive periodontal visits allows for early intervention to enable clinician to save an ailing implants.
Primary causes of implant failure have been suggested to be plaque, bacterial infection and traumatic occlusal forces [3]. Some of the bacterial species found to be associated with failing implants are reported to be
Implant is “any object or material, such as an alloplastic material or other tissues, which is partially or completely inserted and grafted on to the body for the diagnostic, prosthetic and experimental purposes” [5]. As defined by Glossary of Prosthodontics terms. In the late 1950s, Per Ingvar Branemark, a Swedish Professor in Anatomy, studying blood circulation in bone and marrow, developed through a serendipitous finding a historical breakthrough in medicine he predictably achieved an intimate bone to implant apposition that offered sufficient strength to cope with load transfer, he called the phenomenon as “osseointegration” [6].
In 1965 the first patient was treated by means of this approach for a law edentulous jaw. One definition of osseointegration was provided by Albrektson et al. (1981) [7] who suggested that this was a direct structural and functional connection between living bone and the surface of load carrying implants. As defined by Zarb and Albrektson “a process whereby clinically asymptomatic rigid fixation of alloplastic materials is achieved and maintained in bone during functional loading” [8].
A series of screw shaped, commercially pure titanium implants were inserted in the symphysis and left covered for a few months. Commercially pure titanium implants were inserted in the symphysis and left uncovered for a few months. The gingival and mucosal tissues were reopened and titanium abutments were placed, on top of which fixed prosthesis could be screwed. All implants appeared firmly anchored. Since that time millions of people have been treated worldwide using this technique. The implants used sometimes had different geometrics and surface characteristics.
The serendipitous finding of Branemark was that when a hole is prepared in the bone without traumatizing the tissues or overheating, an inserted biocompatible implantable would achieve an intimate bone apposition and micro movements at the interface were prevented during early healing period [6].
A successful outcome of implant therapy depends on number of factors. A satisfactory healing following implant placement is determined by biocompatibility of implant material and surgical technique. Presence of microbes and their interaction with host tissues in the oral environment may result in pathological reaction in the peri-implant tissues and thereby compromising tissue integration. Thus prevention of disease is a key factor in the aim of preserving the supporting tissues around implants.
The integration of hard and soft tissues with implants is the result of wound healing process. A blood clot is formed within of the surgical procedure. After a few days, there is infiltration of the clot by vascular structures and abundant inflammatory cells to form a granulation tissue. The continuation of the healing process involves the organization of connective tissue by modification of granulation tissue. This is followed by the formation of bone which in turn results in osseointegration at the recipient site.
Formation of barrier epithelium adjacent to implants and apical to the epithelium the connective tissue that integrates with titanium surface prevents the epithelial migration. The barrier epithelium and connective tissue/implant interface establish a specific biological width of peri implant mucosa.
The soft tissue that surrounds the transmucosal parts of implant is termed as peri-implant mucosa. The structure and dimension of this mucosa is similar to that of gingiva around the teeth. The concept of biological width controls the thickness of soft tissue adjacent to both teeth and implants. Some fundamental differences also exist between these two tissue types in terms of gingival fibers, periodontal fibers and periodontal ligament space.
In tooth, a layer of cementum covers the surface of root. From the cementum, the collagen fibers run in a perpendicular direction to the long axis of the tooth and insert into the surrounding hard and soft tissues. But, the implant lacks the cemental layer and, hence, collagen fibers are unable to attach to the implant surface in the same way as around the tooth. Thus, the collagen fibers are aligned in different directions and in the tissue which is in immediate lateral surface to the implant surface. The collagen fibers are orientated parallel to the long axis of the implant. Nevertheless, an effective soft tissue seal to the oral environment is provided by the biological attachment formed by the barrier epithelium and the connective tissue in the mucosa surrounding the implant (Figure 1).
Peri-implant mucosa.
Increasing probing depth and loss of clinical attachment are pathognomonic for periodontal disease. Pocket probing is, therefore, a crucial procedure in diagnosis of the periodontium and for the evaluation of periodontal therapy. The major clinical criteria used to determine the success of periodontal treatment are reduction of probing depth and gain of clinical attachment level. The penetration of probe is greatly influenced by various factors such as the roughness of the root surface, the inflammatory state of the periodontal tissues and the firmness of the marginal cuff.
The limitations of probing are – (i) it often fails to identify the histological level of the connective tissue attachment as determined by various studies; (ii) it has a limited reproducibility; variations of 1 mm have to be expected under clinical conditions. The changes that occurred in the past are reflected by measurements of clinical attachment level. Once disease is detectable by clinical attachment level measurement, it is indicative of substantial, and possibly irreversible, tissue changes have already occurred.
Some of the advantages of probing are—the simplicity of the method and the immediate availability of the results. Also, the topographical disease patterns can also be demonstrated. The results from a histological study determining the extent of peri-implant probe penetration in dogs indicate that the density of the peri-implant tissues influences penetration depth. In inflamed tissues around one-stage non submerged implants, periodontal probes penetrated close to the bone level, whereas the probe tips tended to stop at the histological level of connective tissue adhesion if healthy tissues were present [9].
Quirynen et al. [10] found a correlation between the level of bone as seen on radiographs and the extent of peri-implant probe penetration. In the case of screw-type implants, the probe tip appeared to stop 1.4 mm coronally to the bone level. It was observed that the mean discrepancy between probe penetration and the location of the bone margin in radiographs was 1.17 mm in 100 non submerged titanium implants 1 year after implantation. [10] Microbiological studies have shown that there is a marked difference in the composition of the peri-implant microflora between implants with deep and shallow pockets.
Deeper pockets of 5 mm or more can be viewed as protected habitats for putative pathogens and indicate peri-implantitis. The penetration of the probe tip is influenced to some extent by implant shape and surface texture. In some implants, peri-implant probing is impossible due to peculiarities of the shape or design (concavities, shoulders or steps) of the implant. Lack of surface smoothness (such as plasma-coating, sandblasting or the presence of threads) may increase the resistance to probe penetration and may lead to the underestimation of pocket depth. Thus, probing around implant has not gained much acceptance among clinicians as a reliable diagnostic tool.
On the other hand, one may consider it a deficiency of an implant system if its design disables probing. In addition, some authors have expressed concern about the possibility of introducing bacteria into the peri-implant tissues and damaging the implant surface with a metallic periodontal probe while probing. The peri-implant probing should include a fixed reference point on the implant or its suprastructure to measure the relative attachment level. If peri-implantitis is associated with a marginal recession, then probing depth alone may not accurately reflect peri-implant bone loss, whereas increasing loss of attachment is definitely indicative of peri-implant pathology.
The examination of peri-implant tissues is fundamental in the maintenance and follow-up of implant treated patients. The methods to be applied in the clinical examination of the tissues surrounding implants resemble those used in the examination of the periodontal tissues surrounding teeth. Thus, probing represents one of the critical assessments and includes not only the appraisal of probing pocket depth (PPD) but also the more important detection of bleeding on probing (BOP). Probing peri-implant and periodontal tissues is in most respects similar and is regarded as a predictable and reliable procedure in the effort to distinguish between healthy and diseased tissue, provided that a normal force is applied [9].
When probing healthy tissues around implants and teeth, the probe meets resistance from the peri-implant mucosa/gingiva and the apical extension of the probe into the pocket corresponds to the vertical dimension of the junctional epithelium. Probe penetration of inflamed tissues, however, is different such that the probe reaches a position apical to the epithelial extension, depending on the degree of inflammation (Figure 2).
Peri-implant probing.
Definition of inflammatory changes of peri-implant tissues should be based on established periodontal index systems such as the Sulcus Bleeding Index or the Gingival Index. Consequently, a modified Bleeding Index has been proposed by Mombelli et al. [11]. Also, a simplified Gingival Index was suggested by Apse et al. for the assessment of soft tissues around implants [12].
In the Gingival Index scores, two important discriminators used are the texture and color of the gingiva. However, in case of implants, these features depend on the normal appearance of the recipient tissues before implantation. They may also be influenced by the properties of the implant surface. Non-keratinized peri-implant mucosa appears redder than keratinized tissues. Therefore, a modification of the original Gingival Index was required for use on implants.
In a longitudinal study conducted by Chaytor [13], only a weak correlation between the Gingival Index scores and changes of marginal bone level was reported. In clinical practice, the reduction of the evaluation of signs and symptoms of inflammation to bleeding on gentle probing on implants may be a reasonable extrapolation from the clinical situation around teeth. In contrast to gingivitis and periodontitis patients, this parameter has not yet been validated for implant situations.
Consequently, for peri-implant lesions, sensitivity, specificity, diagnostic accuracy and predictive values are not available. However, from a biological point of view it may be reasonable to assume that absence of bleeding on probing represents stability of the peri-implant mucosal seal in a similar way as absence of bleeding on gentle probing represents stability and health in periodontal tissue. Further research in required to fill these gaps in knowledge, of the role of this common clinical parameter as an indicator and/or predictor for health and disease (Tables 1 and 2).
Score 0 | No detection of plaque |
Score 1 | Plaque only recognized by running a probe across the smooth marginal surface of the implant. Implants covered by plasma spray in this area always score 1 |
Score 2 | Plaque can be seen by the naked eye |
Score 3 | Abundance of soft matter |
Assessment of plaque accumulation by a modified Plaque Index [11].
Score 0 | No bleeding when a periodontal probe is passed along the gingival margin adjacent to the implant |
Score 1 | Isolated bleeding spots visible |
Score 2 | Blood forms a confluent red line on margin |
Score 3 | Heavy or profuse bleeding |
Assessment of bleeding tendency by a modified Sulcus Bleeding Index [11].
The establishment and, maintenance of intimate contact between the bone and the implant is a major requirement for implant success. An important criterion for the success of implant therapy is the absence of mobility. Clinically visible mobility of an implant after an appropriate period indicates failure to achieve osseointegration. Presence of mobility at the follow-up visit is a sign of the final stage of peri-implant pathology and indicates complete failure of osseointegration. Implants with less advanced stages of peri-implantitis may still appear immobile due to some remaining osseointegration. Thus, mobility cannot be used to detect early stages of peri-implant pathology. It is advisable to use an electronic device to interpret low degrees of mobility [9].
Peri-implant mucositis is described as the inflammation limited to soft tissues around a dental implant. It may result from dental plaque colonization and is a reversible inflammatory condition. It does not involve any bone loss, analogous to gingivitis around natural teeth. A diagnosis of peri-implantitis results when the inflammation spreads apically, causing progressive loss of osseointegrated supporting bone, analogous to periodontitis around natural teeth. The practitioners essentially require to be familiar with these diagnostic terms when assessing the long-term success of implants and peri-implant health.
It is also important to accurately identify the etiology and chronology of bone loss around implants for better diagnosis and treatment plan. Bone loss may result from surgical trauma or technique, such as pressure necrosis from inadequate osteotomy preparation or coronal bony voids from excessive counter sinking. It must be differentiated from bone loss resulting from bacterial plaque mediated by an immune-inflammatory reaction. Implants placed using a subcrestal platform position have been shown to have a deeper baseline probing depths than those placed supracrestally; thus, it is important to know the baseline probing depth after initial healing to allow monitoring for changes over time.
Various studies in literature have reported the incidence and prevalence of peri-implantitis. Berglundh et al. [14] found that the incidence of peri-implantitis was up to 14.4% and appeared to be related to the number of years for which the fixtures were in service. Additionally, Roos-Jansaker et al. [15] reported that of all implant cases which were not enrolled in a regular post treatment periodontal maintenance program, 16% demonstrated peri-implantitis by 7–9 years after implant placement. The incidence of peri-implantitis may be underestimated because only few studies exist with follow-up longer than 10 years.
Dental implants have been shown to be successful in patients with severe periodontitis. Similarity between the bacterial profile around implants and natural teeth has been demonstrated by several researchers. Moreover, dental implants may harbor a complex microbiota with a large proportion of known periodontal pathogens, which have been associated with the onset of peri-implant mucositis and peri-implantitis [16]. Additionally, long term follow-up studies that examined dental implants in patients with a history of periodontitis, have suggested a higher incidence of soft-tissue inflammation (mucositis) and peri-implantitis, as well as a slightly higher failure rate [17]. These findings suggest that the patients with dental implants require regular and careful evaluation at selected periodontal maintenance intervals to detect any clinical signs and symptoms of peri-implant disease at an early stage.
Probing depths should be recorded to detect the inflammation in the peri-implant mucosa. It helps to identify bleeding or suppuration during examination. While the probing pocket depth (PPD) may vary around implants, such assessments are secondary to bleeding on probing (BOP). Sites with PPD ≥ 6 mm, however, may indicate pathology and thus require meticulous examination. For peri-implantitis, bone loss can be assessed through radiographs in addition to the PPD and BOP. The radiograph for this purpose should be obtained after the delivery of the prosthesis [18].
The similarity between inflammatory lesions in peri-implant mucositis and gingivitis has been revealed by various animal experiments and analyses of human biopsy material. The development of inflammatory lesions in the connective tissue in the marginal portion of the gingiva or peri-implant mucosa as a response to microbial challenge follows the same pattern and the composition of inflammatory cells in both the lesions. While gingivitis and mucositis are reversible conditions, periodontitis and peri-implantitis are not. The inflammatory lesion in the former conditions can be completely resolved after the institution of appropriate infection control measures [14, 19] (Figure 3).
Peri-implant mucositis.
Peri-implantitis lesions differ from mucositis lesions in that they exhibit characteristics that are markedly different from their periodontal counterparts. The inflammatory lesion in periodontitis is contained within the sub-epithelial connective tissue compartment of the gingiva and is separated from the alveolar bone by a 1 mm-wide zone of dense connective tissue. Furthermore, the area of soft tissue affected with pocket formation is lined by a pocket epithelium. The epithelium in its most apical portion is in contact with the root surface and thereby effectively sheds off the biofilm of bacteria in the pocket.
In peri-implantitis, bacteria survive in the inflammatory lesion within the pocket compartment. But the entire extension of the pocket usually remains uncovered by a pocket epithelium. Thus, the apical third of the inflamed tissue in the pocket comes to lie in direct contact with the biofilm. Another dissimilarity to periodontitis is the extension of the lesion in peri-implantitis. The lesion in peri-implantitis is seen to extend to a position closer to the bone surface, while the lesion in periodontitis is usually separated from the crestal bone by a zone of connective tissue. An understanding of the difference in the lesions observed in periodontitis and peri-implantitis will help clinician to select the appropriate treatment strategy (Figure 4).
Peri-implantitis.
Following the completion of the surgical and prosthetic procedures in implant therapy, it is imperative to inform the patient about the self-performed infection control procedures [20]. Different types of toothbrushes and/or floss are available to suit the varying designs of the prosthetic reconstruction. The patient should be taught to use the mechanical cleaning aids properly and efficiently to clean the implant and adjacent parts of the prosthesis. The cleansing should be performed twice a day. The prosthesis should be designed in a such way as to allow access for self-performed and professional infection control [20].
The implant sites should be evaluated with radiographs carried out at two time points - at the time of the delivery of the prosthesis and at the one-year follow up. Any alteration in the marginal bone level should be recorded during the first year in function of an implant. This change may be associated with the remodeling of bone after implant installation. This information will serve as a baseline value for evaluation of bone level at subsequent visits.
The following radiographic parameters are in currently in use for evaluation of dental implants:
Assessment of alterations in height of peri-implant bone
Computer-assisted evaluation of changes in peri-implant bone height
Assessment of quality of peri-implant bone
Photodensitometric evaluation of peri-implant bone quality
Bone mineral content (dual-photon absorptiometry)
Clinical examinations should be performed at all annual follow- up visits. Besides examining the function of the prosthesis, BOP, PPD and plaque assessment should also be carried out. If the probing indicates peri-implant disease (BOP positive and PPD ≥ 6 mm), a radiographic examination is called for to reveal possible bone loss. In the absence of clinical findings of pathology in peri-implant tissues, radiographic examination should be avoided.
The re-evaluation of implant-treated patients should be designed in accordance with evaluation of risk factors for peri-implant disease. Subjects with a history of severe should be recalled in every 2–6 months after the delivery of the prosthesis. Routine maintenance therapy is imperative for maintenance of the peri-implant health. Implant maintenance therapy includes considering the patient’s overall health in addition to the assessment and monitoring of implant(s).
Implants fail from a loss of integration generally due to bacterial infection, occlusal overload, or a poorly designed prosthesis. The role of a dental hygienist and dentist is thus essential in preventing and controlling bacterial infection, including careful instrumentation and polishing of implant(s) once in every 3-4 months.
At each appointment, medical history and overall health of the patient should be updated and reviewed. Any changes in the health status of the patient can influence negatively the success of implants or treatment provided. If the diabetic status of the patient is not under good control, this can increase the risk of peri-implantitis and ultimately implant failure. Overall good general health is one of the keys to the success of the implant(s).
Implant dentistry is true interdisciplinary dentistry, requiring close collaboration with the surgical practice, the dental laboratory, and the patient’s physician [20].
Implant assessment starts with a visual soft tissue examination of the peri-mucosal seal and should be carried out at every maintenance appointment. Any signs of inflammation or bleeding or suppuration should be recorded. It is important to record any clinical symptoms present, such as pain and mobility of the implant. Obtaining accurate radiographs will enable the clinician to evaluate the crestal bone level appropriately [20].
The soft tissue should be examined for color, texture, form, bleeding, and inflammation. The assessment and any tissue changes should be recorded and photographs should be taken. This photograph or digital image can be used to educate the patient and can be an excellent visual tool to reinforce the importance of good home care.
Soft tissue assessment includes redness, inflammation, or bleeding, check for the presence of calculus deposits around the implant. Peri-implant infections can progress more rapidly than infection around natural teeth. In presence of an infection, the dental hygienist or dentist will evaluate for pain, mobility. All the data that is gathered is made available to the dentist to develop a treatment plan. The plan may include shortening the interval between implant maintenance visits, possible antibiotics, a radiograph, and/or the dentist may refer the patient for an evaluation by a specialist.
Some researchers recommend not to probe around the implant, or wait for 3 months, following abutment attachment, to avoid disrupting the formed peri-mucosal seal. The peri-mucosal seal is fragile and probe penetration induces pathogens and jeopardize the success of the implant, a number of considerations and guidelines should be followed when probing the tissue surrounding an implant. A flexible plastic probe is recommended to avoid any scratching of the implant’s surface and reduces the potential for trauma to the peri-mucosal seal. Secondly, the probe should be used as a measuring device for recording inflammation or to measure exposed implant threads for monitoring.
A baseline measurement should be established by identifying a monitor marker on the restoration and should be gently probed to check the clinical parameters. This information should be recorded in the patient’s notes along with any signs of inflammation present at the first implant maintenance appointment (3 months following prosthesis placement).
Presence of infection, pain, mobility, or unacceptable bone loss are the signs of failing implants. Pain or discomfort may be the important signs, before it is evident on a radiograph. In presence of pain, the dentist needs to evaluate the cause i.e. whether it is due to occlusal trauma or infection. An occlusal adjustment may be required to be performed since an implant is held in place by bone not by the periodontal ligament and does not respond like a natural tooth to occlusal trauma.
Mobility following osseointegration can occur because of a loose fixed restoration, infection, fractured abutment thread, an implant fracture or trauma. In case the mobility is due to a loose crown, it may be possible to re-cement it or rescrew it (depending on the type of abutment). If mobility of the implant itself or a broken screw, this is a greater cause for concern. A radiographic assessment is needed.
This final step in monitoring the dental implant(s) is the radiographic assessment using a measurable device is recommend to accurately monitor the crestal bone level around the implant(s) and to verify that the restoration is seated properly. The abutment can be visually confirmed through indentations in implant shown in the radiographs, or the screw that is clearly in focus, which should appear as a clear line. This is indicative of a properly seated abutment.
Further radiographs can be taken to determine any crestal bone loss around the implant and to measure the same if present. A measurement of 0.5–1 mm. horizontal bone loss is acceptable in the first year, with an anticipated 0.1 mm of bone loss each subsequent year. If more than 1 mm of horizontal or vertical bone loss is detected in the first year, an evaluation by the implant surgeon is recommended.
Following careful assessment of implant, the dental hygienist or dentist should ascertain the presence of calculus on the implant or abutments. Minimal, or indeed no, instrumentation is required for an implant with a healthy gingival attachment. Calculus or microbial deposits are primarily supragingival and are safe for instrumentation. Care must be taken to avoid scratching or roughening the implant surface, as this may result in bacterial accumulation and subsequent inflammation [20].
There is a difference in instrumentation around an implant and natural tooth. Natural teeth are anchored in the bone by the periodontal ligament and sulcular epithelium, whereas the implants are osseointegrated to bone. For instrumentation of a natural tooth, the instrument blade is adapted to the tooth surface and gently inserted between the sulcular epithelium and the side of the tooth or root. To remove calculus deposits, vertical, horizontal, and oblique stokes are used.
A thorough instrumentation of implants requires the removal of microbial deposits without creating any alteration of the surface of implant or adversely affecting its biocompatibility. Scratches and gouges may be created on the surface that will affect the titanium-oxide layer, reducing the corrosion-resistant nature of a titanium implant. The implant surface may also get contaminated with trace elements from the remaining scaler material, which compromises the long-term osseointegration of the implant.
The suggested materials for use on implant surface are plastic, graphite and titanium scalers. Some studies had revealed that these instruments do not scratch or gouge implant surface. Titanium is the metal of choice because it produces instruments which are thinner than plastic or graphite instruments and provides more strength to dislodge calculus. They are also more biocompatible with other metals. This avoids leaving trace elements from a scaler on the implant surface.
According to Dmytryk, Fox and Moriarty [21],
The results of these research studies throw light on the fact that more studies are needed to evaluate the effects of debris left behind on the implant surface, and the biocompatibility of this debris with the titanium implant surface. Stainless steel instruments and metallic power scaler tips have been shown to gouge or scratch the implant surface and are therefore contraindicated. However power scalers and air powder abrasive systems can be used with specific tips, sleeves and powder formulated for implants. Care should be taken when using a plastic sleeve with the tip of a power scaler to prevent aspiration of the plastic tip, in case it gets dislodged [19].
Implant Prophy™ from TESS are designed from materials like polycarbonate plastic and include Gracey and Columbia designs. Implacare™, Hu-Friedy instruments are featured with a sturdy handle and plastic disposable tips in various designs. Premier Dental Facial implant scalers are made of non-metallic, autoclavable graphite. Titanium-coated Suvan-O’Hehir implant scoop curettes are available from G. Hartzell and Son [20].
An instrument called Implant Pro™ from Brasseler is available in the Langer series with titanium tips that can be replaced from time to time. Nordent makes ImplaMate™, also in the Langer series, Barnhart and universal scalers. The newest in the market are the Wingrove Series, made by Paradise Dental Technologies (PDT), which are designed with a uniquely processed titanium that will refrain from any scratches or leaving any debris behind on implants. These are available in a series of three professionally designed scalers which can be adapted to specifically meet all the challenges of maintaining an implant.
Few of the challenges include removing calculus from a variety of implants and restorative choices. Some are narrow base implants (narrow platform is used for lower incisors, congenially missing laterals, and area with limited available bone) while others have a wide base or wide platform. There is difficulty to gain access to high water bridges as well as full-arch cement or screw retained implants. Also an instrument with small diameter is required to fit under a Hader clip bar or around O-ring ball or locator abutment that can be used for debridement of over dentures.
It is very critical to select an appropriate instrument to remove calculus deposits that will not harm the implant surface during debridement. For narrow base posterior implants or implants that replace two adjacent teeth, an instrument with a longer blade will be advantageous. It can be used under the more bulbous shaped crowns and even under the framework of a high water bridge or full arch implant retained prosthesis. The scaling stroked should be short and horizontal. The calculus present on these implants, crowns or frameworks can be dislodged effectively.
For wide base posterior implants, a universal posterior implant scaler is recommended with short vertical strokes to remove the calculus. For instrumenting any exposed implant threads; anterior or posterior, a shorter radius blade tip of an instrument is suggested which can be used carefully in a side-to-side motion, covering one thread at a time.
For patients with over denture implant abutment, the denture has to be removed to assess the O-rings or clips inside the denture for loss or wear. These O-rings or plastic retention clips should be replaced if worn out, or replaced at least once a year.
For instrumenting the abutments under an over denture, a thinner radius blade tip is adapted under a Hader clip bar in a side to side stroke. An instrument tip with a shorter radius is recommended, used with short vertical strokes around a ball or locator abutment to dislodge any calculus. It is important to understand the unique and different designs of implant and to have a proper armamentarium for the safe implant maintenance. This will allow the clinician to provide patients with ideal implant care with a predictable long-term success of their implants [20].
Soft rubber tip, with appropriate nonabrasive paste to polish the implants. Aluminum oxide, tin oxide, APF-free prophy paste, and low-abrasive dentifrice are all considered acceptable polishing abrasives for implants. Coarse abrasive polishing pastes and acidulated phosphate fluoride (APF) products are contraindicated, as they may etch surface of implants.
Conventional periodontal therapy should be done when inflammation develops around an implant. The therapy should include the efforts to improve patient’s oral hygiene, with methods similar to those used for natural teeth. Lang et al. [22] suggested a novel, systematic stepwise approach for the prevention and treatment of peri-implant diseases. This approach is referred to as the cumulative interceptive supportive therapy (CIST) protocol.
It is based on periodic monitoring with implementation of treatment as thresholds for a particular condition are met. The first step is protocol (A), then (B) and, if conditions continue to worsen, the patient may require more advanced treatment, i.e. execution of protocol (C), and finally (D) by a specialist who has implant training for it. To control inflammation in peri-implant mucositis, that is, implants with minimal increase in pocket depth, slight (+) bleeding on probing, marginal erythema, plaque, and/or calculus, Protocol (A) is implemented.
The endpoint of the therapy is resolution of inflammation with careful mechanical debridement (using plastic curettes and rubber cup prophylaxis), swabbing with 0.12% chlorhexidine twice daily, and a review of home care and patient motivation. Protocol (B) is carried out for conditions that exhibit features similar to mucositis but with deeper pocket depths (4–5 mm) but without loss of supporting bone.
The treatment should include the therapies of protocol (A), plus locally delivered antibiotic (minocycline microspheres, doxycycline gel) at the infected implant site(s). Studies in the recent past have shown the use of minocycline microspheres may be beneficial in treatment of peri-implant mucositis and peri-implantitis. For management of early peri-implantitis, Protocol (C) is used, which consists of a more intensive approach and is used in conditions where there is radiographic evidence of osseointegrated bone loss of <2 mm and probing pocket depths >5 mm. The strategy should comprise a combination of the modalities for protocols (A) and (B) with the addition of systemic antibiotic therapy (metronidazole 250 mg
Furthermore, periodontal surgical access for surface de- contamination (citric acid 1–2 min or tetracycline 250 mg, 5 mL for 5 min) should be considered. In cases of frank peri-implantitis that reveal probing depths (>5 mm), (+) bleeding on probing, plaque/calculus, and peri-implant bone loss of >2 mm, Protocol (D) is initiated along with other three protocols.
This treatment modality comprises periodontal surgical intervention for chemical disinfection, osseous resection, and/or guided bone regeneration (GBR). GBR is a procedure to attempt for salvaging the implant through bone regeneration techniques with the use of resorbable or nonresorbable semipermeable membranes and a bone substitute or replacement graft (such as freeze-dried bone allograft or anorganic bovine bone). In clinical practice, the protocol of CIST is targeted for early detection and methodical sequential treatment, which may help rescue and even reverse the fate of the ailing or failing endosseous dental implant [23] (Figure 5).
CIST protocol.
Antiseptic therapy, CIST protocol A & B
Antibiotic therapy, CIST protocol A + B
Antibiotic therapy, CIST protocol A + B + C
Regenerative or resective therapy, CIST protocol A + B + C + D.
Polycystic ovarian syndrome (PCOS) is believed to be the most common cause of infertility in reproductive age women [1]. Although men lack ovaries, there is also a syndrome called male PCOS featuring a similar set of cardiometabolic indicators and risks to that seen in female PCOS as well as early balding [2]. Most people with PCOS are insulin resistant/hyperinsulinemic [3]. When overweight or obesity is present in people with PCOS (as is the case in most people with this disorder) insulin resistance/hyperinsulinemia is exacerbated [3]. Several mutations are associated with an increased risk of PCOS [4]. Gene methylation and histone acetylation abnormalities as well as certain non-coding RNAs may also contribute to the expression of PCOS [5].
Autoimmune disease has been shown to play a role in some people with PCOS, severely insulin resistant diabetes, acanthosis nigricans, and systemic lupus erythematosus with nephritis [6]. Besides these rare and dramatic examples of the Type B syndrome, there is some evidence that an autoimmune process triggered by low progesterone levels may be a trigger for PCOS in many women [7]. A similar phenotype without lupus or anti-insulin receptor antibodies, but with insulin receptor mutations or abnormal post-receptor signaling has also been described [6].
Parasitoses, which are especially common in the tropical and subtropical parts of the world (and becoming more common as a result of the climate crisis), can induce PCOS by virtue of their ability to synthesize steroid hormones, e.g., estradiol and 1,25-OH2-vitamin D3 from its precursor, 25-OH-vitamin D [8].
Alterations in the microbiome have been reported in people with PCOS which may play a causative role [9, 10, 11].
Endocrine disruptor chemicals (EDCs), which are ubiquitous and increasing in our environment, including certain drugs, may also contribute to the pathogenesis of PCOS [12, 13].
Epilepsy has been cited as a cause of PCOS, however, there is controversy as to whether the disorder itself, treatment with valproate, or both are responsible [14].
Many drugs are associated with an increase in insulin resistance (IR) which may be an initial step in PCOS pathogenesis. In addition, EDCs in our environment may initially cause IR or bind as agonists to estrogen or androgen receptors, eventually contributing to PCOS [15].
In the remainder of this chapter, I shall review what is known about these diverse contributing causes of PCOS in the hope that in so doing clinicians might explore these often-reversible factors in their patients. I further hope that such a review may point to common pathogenic pathways in many, if not all, people with PCOS. Finally, I hope that appreciation of the various causes of PCOS can lead to improved preventive strategies and individualized, precision treatment of people with PCOS.
The marked tendency of PCOS for familial clustering (made even more remarkable by the hypo-fertility of people with PCOS) has long supported the notion that PCOS has a genetic component. However, since families often share similar diets, lifestyles, and EDC exposures, twin studies using monozygotic twins raised in very different environments would be helpful in separating genetic and environmental effects. Although it was shown that the tetrachoric correlation for PCOS in monozygotic twin sisters is higher than for dizygotic twins or for non-twin sisters, each set of twins or sisters in this large study was brought up in the same family. Despite efforts of most parents to raise monozygotic twins as distinct individuals, they are apt to, nonetheless, share a more similar environment and set of experiences than less closely related siblings, leaving open the possibility that shared environment/experience contributes significantly to the correlation [16]. In addition to tetrachoric correlation, both univariate analysis and a trivariate genetic analysis of major findings occurring in women with PCOS suggested a strong genetic component of PCOS in this Dutch twin study by Vink and colleagues [16]. Other twin studies in people with PCOS have reached similar conclusions [17, 18, 19, 20].
Genome-wide association studies (GWAS) have been helpful in identifying polymorphisms that are associated with an increased risk of PCOS development [21, 22, 23, 24]. Nevertheless, only about 10% of the apparent heritability of PCOS to date can be explained by these associations, leading to speculation that various phenotypes are associated with rare polymorphisms. Newer technologies e.g., gene and whole exome sequencing may clarify the contribution of rare polymorphisms to different phenotypes in the future [21].
Among the GWAS-identified candidate loci are DENND1A, LHCGR, INSR, FSHR, ZNF217, YAP1, INSR, RAB5B, and C9orf3 [22]. Polymorphisms found in DENND1A (P = .0002), THADA (P = .035), FSHR (P = .007), and INSR (P = .046) in Chinese women with PCOS were also strongly associated with PCOS in European women [24].
GWAS often fails to identify candidate loci in the mitochondrial portion of the genome [25]. Recent publications suggest that the mitochondria may play a pivotal role in PCOS pathogenesis, both genetically and epigenetically, given the essential mitochondrial role in cellular metabolism and IR. Recently Ye and colleagues reported that a 4977 base pair deletion in mitochondrial DNA detected in peripheral blood using multiplex probe-based qPCR was highly associated with PCOS in a logistic regression analysis [26]. In a study by Saeed and colleagues it was reported that most of the mitochondrial DNA mutations (80%) were limited to a 3157–3275 base region which is evolutionarily conserved and would be expected to change the secondary structure of mitochondrial transfer RNAs. As suspected, 6 mutations (A to G and/or T to C) altered the expected base pairing. Mitochondrial DNA copy numbers were also diminished in women with PCOS compared with controls [27]. Zeng et al. have reviewed the role of oxidative stress (OS) in people with PCOS [28]. They summarized much of what is currently known about the role of mitochondrial dysfunction in PCOS. Reduction of mitochondrial DNA copy number and mitochondrial mutations contribute to IR, metabolic syndrome, and disordered development of ovarian follicles through increased production of reactive oxygen species (ROS). Obesity plays a pivotal role in the pathogenesis of PCOS in most people, however, mitochondrial genome alterations related to PCOS with obesity are not yet well understood, underlining the need to investigate changes in the mitochondrial genome that are associated with obesity. External environmental factors may also disrupt mitochondrial function. Recent attention has focused on the effect of environmental factors e.g., cigarette smoke and bisphenol A on reproduction. Cigarette smoke has been reported to disrupt ovarian development; 1-(N-methyl-N-nitrosamino)-1-(3-pyridinyl)-4-butanal (NNA), contained in third-hand smoke, reduced ovarian weight and follicle number in rats exposed to NNA for 30 days compared with controls and even had a serious negative effect on development of the offspring of NNA-exposed rats. These adverse reproductive effects of cigarette smoke seem to be due to mitochondrial dysfunction. NNA exposure causes ROS buildup by increasing superoxide dismutase (SOD) mRNA levels, inducing apoptosis. Benzo(a)pyrene (BaP), another component of cigarette smoke, causes massive mitochondrial ROS leakage/dysfunction, resulting in significant plasma membrane lipid peroxidation and disrupted ovum fertilization. Cigarette smoke also adversely effects the development of granulosa cells, which have an essential role in providing optimal amounts of the hormones and nutrients needed for follicular development.
These include abnormalities of DNA methylation, histone acetylation, and downstream signal transduction abnormalities.
Epigenome-wide association studies (EWASs) are helping in the discovery of environmentally mediated molecular changes in PCOS from disease pathogenesis to the discovery of epigenetic markers. Recent epigenetic studies offer persuasive evidence linking epigenetic regulation with PCOS etiology, presentation, clinical phenotypes, and comorbidities, which could potentially lead to improved disease prevention and management via precisely targeted strategies. Several pivotal biological pathways have been repeatedly reported by independent groups, supporting functional regulation by endocrine abnormalities and metabolic dysfunction in PCOS, while also suggesting an autoimmune component in the syndrome [29]. Increasing application of high-throughput sequencing technologies for epigenome analysis combined with evidence-based causal inference should facilitate precision PCOS prevention/treatment in the future.
Vázquez-Martínez et al. recently reviewed the topic of DNA methylation in women with PCOS [5]. Alterations in DNA methylation, histone acetylation and noncoding RNAs have been found in diverse tissues of women with PCOS. DNA methylation abnormalities appear in peripheral and umbilical cord blood, and in ovarian and fat tissue of women with PCOS, suggesting a pivotal role for these epigenetic modifications in the pathogenesis of this disorder. Possibly, these derangements in DNA methylation facilitate deregulation of gene expression involving inflammation, hormone biosynthesis and signaling, as well as glucose and lipid metabolism. The authors have compiled an extensive table of the tissues in which methylation abnormalities are encountered in women with PCOS indicating whether the involved DNA is hypo- or hypermethylated, the changes in gene expression, if any, related to the methylation variants, and any documented clinical/phenotypic expression resulting from these changes. Interestingly, both hypomethylation of some genes and hypermethylation of others may predispose to PCOS.
Qu and colleagues studied the effects of hyperandrogenism on the expression of histone deacetylase 3 (HDAC3), peroxisome proliferator-activated receptor gamma 1 (PPARG1), and nuclear corepressor 1 (NCOR1) genes in the granulosa cells of women with a hyperandrogenic form of PCOS, compared with women with non-hyperandrogenic PCOS, women without PCOS who had tubal infertility, and a rodent model of PCOS [30]. NCOR1 and HDAC3 mRNA expression was higher in the hyperandrogenic women than in normo-androgenemic women with PCOS and controls (P < 0.05). When all women were divided into successful and failed pregnancy subgroups, they found lower PPARG1 mRNA levels and higher NCOR1 and HDAC3 mRNA levels in the failed subgroup with hyperandrogenic PCOS (P < 0.05). Two hypermethylated CpG loci in the PPARG1 promoter and 5 hypomethylated CpG loci in the NCOR1 promoter were encountered only in the hyperandrogenic women with PCOS (P < 0.01–P < 0.0005). The acetylation levels of histone H3 at lysine 9 and p21 mRNA expression were low in human granulosa cells cultured with dihydrotestosterone in vitro (P < 0.05). A PCOS rodent model also displayed abnormal PPARG1, NCOR1, and HDAC3 mRNA expression and methylation alterations of PPARG1 and NCOR1, consistent with those found in women with hyperandrogenic PCOS. A strength of this study is the consistent effect of hyperandrogenism in the induction of epigenetic changes in PPARG1, NCOR1, and HDAC3 in granulosa cells in hyperandrogenic women and rodents with PCOS as well as in vitro, which have a role in the ovarian dysfunction encountered in women with a hyperandrogenic PCOS phenotype.
When considering our genome and our epigenome we often lose sight of the fact that the organisms that live within us and on us, though having a different number of chromosomes than the cells we think of as human with somewhat different gene sequences, contribute to our total genome and epigenome. In sheer number, the cells of our biome far exceed the number of cells we think of as human. The character and density of their gene products profoundly influence our hormonal, metabolic, and immune milieu, and even our mood and personality. In the case of parasites, they are in turn hosts to biomes of their own.
As mentioned in the Introduction, we have published the case history of a woman who had PCOS associated with extensive neurocysticercosis [8]. She had refused standard treatment with albendazole for her parasitosis (which she presumably acquired in her native Mexico) because of fear of drug side effects that some of her affected friends had experienced. She had been referred to our clinic because of complaints of worsening hirsutism and amenorrhea x 2 years. She was 32 years old G1P1001. Diagnostic work-up fulfilled Rotterdam criteria for PCOS with amenorrhea, hirsutism, low sex hormone binding globulin, and an elevated LH/FSH ratio. Non-classic adrenal hyperplasia, pregnancy, and virilizing tumors were excluded by appropriate tests. Hypovitaminosis D was excluded by measurement of vitamin D metabolites, however, her serum 1,25(OH)2-vitamin D3 level was elevated. Treatment with lifestyle modification (weight loss diet, prescribed exercise), and gradually up-titrated doses of metformin to 2000 mg/day was associated with a gradual reduction in hirsutism and a return of menses, although still with oligomenorrhea. SHBG rose slightly and there was normalization of the LH/FSH ratio.
We wanted to know whether her extensive burden of neurocysticercosis was playing a role in the etiopathogenesis of her PCOS, perhaps by pressing on the GnRH cells of the hypothalamus, however, the neuroradiologist could find no evidence of anatomic hypothalamic involvement by the encysted parasites. We also considered the possibility that her elevated serum 1,25-(OH)2-vitamin D3 elevation was due to the formation of granuloma-like lesions around the encysted parasites with either the encysted parasites or the surrounding mononuclear cells synthesizing 1,25(OH)2-vitamin D3 in excess, as occurs in other granulomatous disorders like pulmonary sarcoidosis and tuberculosis. We also performed a literature search for associations between cysticercosis and PCOS. While we did not find any reports of such an association, we did learn that Taenia sp. prefer female to male hosts, and pregnant to non-gravid hosts [31, 32, 33]. It was later learned that Taenia sp. have steroidogenic enzymes and can synthesize steroid hormones e.g., estradiol [34, 35, 36, 37, 38, 39]. As the cysticercosis burden increases, the host, whether female or male, will be further estrogenized, rendering the host milieu more favorable to the parasites. While PCOS is correctly considered a hyperandrogenic condition in most women, it is also important to remember that it is also a state of unopposed estrogen effect in anovulatory or oligo-ovulatory women. The sustained estrogen effect would be conducive to Taenia parasitization and increasing cysticercosis burden. In addition, Taenia sp. can metabolize the relatively weak androgen, androstenedione, to the more potent androgen, testosterone [34].
In searching further, we learned that the selective estrogen receptor modulator (SERM), tamoxifen, had successfully reduced cysticercosis burden in a murine model [40]. Since our patient continued to decline standard treatment for cysticercosis we offered her a trial of treatment with another SERM, raloxifene, which did not carry the risk of estrogenic endometrial stimulation reported with tamoxifen [41]. We thoroughly reviewed the article by Vargas-Villavicencio et al. with our patient and carefully explained that raloxifene was an approved and generally safe drug in the US for the treatment of post-menopausal osteoporosis/osteopenia, but not for neurocysticercosis. We explained that it was similar to, but distinct from and safer than the tamoxifen used in that article. We emphasized the importance of avoiding conception during the trial using abstinence or reliable barrier contraception as this was an FDA Category X drug (should not be used in pregnancy). We obtained her informed consent and initiated treatment with raloxifene at the standard dose for osteoporosis/osteopenia of 60 mg/day. When she returned to clinic, about 7 weeks after starting raloxifene, she related that she thought she might be pregnant and that, despite being forewarned, she had had unprotected intercourse on a few occasions. Pregnancy was confirmed by physical examination and serum HCG level, and she was counseled on her options. She elected to terminate her pregnancy. Following termination, a repeat brain MRI was performed. It was read by the same neuroradiologist who had read her baseline study. He was blinded regarding her treatment between the 2 studies. On the repeat study the total number of encysted lesions fell from 37 to 33, 10 lesions shrunk, 5 disappeared, 18 were unchanged, 4 enlarged and 1 new lesion appeared. Subsequently, after the patient belatedly agreed to and underwent standard treatment with albendazole and dexamethasone, serum 1,25-(OH)2-vitamin D3 fell from 81 to 41 pg/ml while 25-OH-vitamin D level only fell from 34 to 30 ng/ml. This reduction in calcitriol level occurred even though dexamethasone has been reported to increase the serum concentration of this metabolite [42].
This was the first case to be reported of human neurocysticercosis wherein modification of the hormonal milieu was associated with a reduction of cestode burden. The pregnancy on raloxifene, though unfortunate, supported the concept that neurocysticercosis contributed to the pathogenesis of her PCOS. Serum 1,25-(OH)2-vitamin D3 may ultimately prove to be a useful biomarker for assessing disease activity in neurocysticercosis, as it is in several other granulomatous disorders [43]. This report and the preclinical reports preceding it conceptually opened the field of biome contribution to endocrine disorders.
Yurtdaş and Akdevelioğlu recently reviewed the literature on the gut biome and PCOS [44]. While genetic, neuroendocrine, epigenetic and metabolic factors are reported to contribute to the pathogenesis of PCOS, knowledge of the etiologies of the syndrome(s) remains incomplete. Recently, studies in humans and preclinical models have found associations between alterations in the gut microbiome and the metabolic/clinical features of PCOS.
It is theorized that gut dysbiosis could be a pathogenetic factor in PCOS. Accordingly, changing the gut microbiome using probiotics, prebiotics, and synbiotics as well as diet may serve as a new therapeutic modality for PCOS. Specific changes of the gut microbiome in women with PCOS are apparently associated with distinct PCOS phenotypes. Several recent studies indicate that IR, sex steroid concentrations, and obesity alter the quantity, diversity and species composition of gut bacteria in women with PCOS (and vice versa).
Liang and colleagues studied gut biome dysbiosis in PCOS in association with obesity [45]. They recruited 8 obese women with PCOS, 9 lean women with PCOS, and 9 lean control women. Gut bacterial composition was assessed by PCR. Obese women with PCOS were found to have lower observed bacterial structural variants (SVs) and alpha diversity (a composite of different measurements that estimate diversity in a single sample) than the control group, higher beta diversity (a measure of the similarity/dissimilarity of 2 communities) than the lean PCOS group (P < 0.05), and lower abundances of genera (particularly butyrate producers). Regression analysis demonstrated that decreased abundances of several bacterial genera correlated with higher serum testosterone and impaired glucose tolerance. PCOS was associated with alterations in the gut microbiome population. Obesity appears to have a critical role in the development of a dysbiotic gut microbiome in women with PCOS.
Lindheim et al. studied associations between changes in the gut microbiome composition and gut barrier function and metabolic and reproductive abnormalities in women with PCOS [46]. Gut microbiome composition was assessed in stool samples from women with PCOS (n = 24) and healthy control women (n = 19) using 16S rRNA gene amplicon sequencing. Processing of data and microbiome analysis were performed in mothur and QIIME utilizing differing relative abundance cut-off points. Integrity of gut barrier function, inflammation, and endotoxemia were assessed using serum and stool indicators. Correlations with anthropometric, metabolic, and reproductive measures were then calculated. The stool microbiome of women with PCOS demonstrated lower bacterial species diversity and an altered phylogenetic mix compared with controls. The authors did not find significant differences in any bacterial taxa with a relative abundance>1%. Among rare bacterial taxa the relative abundance of those from the order ML615J-28 (phylum Tenericutes) and from the family S24-7 (phylum Bacteroidetes) was significantly lower and was associated with unfavorable reproductive parameters in women with PCOS. Women with PCOS showed alterations in some, but not all markers of gut barrier function and endotoxemia.
Women with PCOS had less species diversity and an altered phylogenetic mix in their stool microbiome, which was associated with certain adverse clinical parameters. Gut barrier malfunction and endotoxemia were not pivotal factors in these women, however, they may contribute to the particular phenotype seen in some people with PCOS.
Given the accumulating data that the gut biome population contributes to the etiopathogenesis of PCOS it seems intuitive that “normalizing” the gut biome in the most rapid way possible, fecal transplant from a “healthy” woman to a woman with PCOS, might effect the most rapid amelioration of the syndrome with the least risk. Such an approach has been dramatically successful in treating pseudomembranous colitis [47]. Although there are no human studies to date, a small study assessing fecal transplant to treat PCOS in a rodent model has been reported with encouraging results [48]. This same study also found amelioration of PCOS in the model with isolated Lactobacillus transplantation.
While a relatively short term improvement in the gut biome is usually sufficient to treat antibiotic dysbiosis-related conditions like pseudomembranous colitis, more chronic conditions, like PCOS, metabolic syndrome, Type 2 diabetes, and inflammatory bowel disease seem to require long term lifestyle changes e.g. shifting from a Western-style diet high in sucrose, animal fat, and animal protein to a prebiotic/probiotic rich, lower calorie, phytonutrient-rich, mostly plant-based diet and an increased amount of regular exercise in order to sustain the improved gut biome and remission of the disorder being treated [49, 50]. Plant-based diets of this type are accompanied by reduced inflammation, less gut permeability, reduced generation of reactive oxygen species (ROS), and improved insulin sensitivity.
Certain drugs, chiefly those used to treat obesity, prediabetes, and T2DM are known to alter the gut biome favorably, while others, the best known of which are antibiotics, may cause dysbiosis with unfavorable metabolic consequences [51, 52, 53]. Among the drugs with beneficial gut biome effects which explain at least part of their clinical actions are metformin, the alpha-glucosidase inhibitors, the GLP-1 receptor agonists, and the dual GLP-1/GLP-2 receptor agonist, tirzepatide.
In addition to diet and drugs, bariatric (metabolic) surgery may affect the gut biome [54]. The taxonomic make-up of the gut bacterial microbiome is significantly affected by metabolic surgery. The most frequent alteration reported in most pre-clinical and human studies is a relative decline in abundance of Firmicutes with an increase in Bacteroidetes, Proteobacteria, and its class Gammaproteobacteria (order Enterobacteriales, family Enterobacteriaceae, genus Escherichia). Interestingly, the gut microbiome population differs substantially in rodents and humans. Proteobacteria increase after metabolic surgery due to a higher gut lumen pH and higher levels of dissolved oxygen that favor growth of facultative aerobic bacteria and inhibit growth of anaerobic bacteria. Reduction in stomach volume after bariatric surgery increases luminal gastric and distal gut pH, resulting in altered bacterial populations and overgrowth. More alkaline gut pH favors growth of
While far more research has been reported on the contributions of the gut biome to the pathogenesis and maintenance of PCOS, recently the possible role of the vaginal biome in PCOS has come under scrutiny [10]. Hong and associates obtained vaginal swabs from 39 women with recently diagnosed PCOS and 40 women without PCOS and compared them using 16S rRNA gene sequencing in a case control study. Screening values for possible bacterial biomarkers of PCOS were analyzed by receiver operating characteristic (ROC) curve methodology. There were significant differences in the vaginal biome bacterial populations between the 2 groups. The vaginal bacterial species in the PCOS group were more diversified than those in the control group (Simpson index of the PCOS group vs. the control group: median 0.49 vs. 0.80, P = .008; Shannon index: median 1.07 vs. 0.44, P = .003; Chao1 index: median 85.12 vs. 66.13, P < .001). This is in marked contrast to what has been reported for the gut biome, which is less diverse in women with PCOS, obesity, and T2DM than in healthy control women. Relative abundance of
The oral cavity biome has also been explored in terms of PCOS [11]. This study was designed to investigate the hypothesis that the concentrations of suspected periodontal pathogens in saliva and the host serum antibody response is elevated in women with PCOS, compared with healthy controls. In total, 125 women in 4 groups were studied: 45 with PCOS+healthy periodontium, 35 with PCOS+gingivitis, 25 systemically and periodontally healthy women, and 20 systemically healthy women with gingivitis.
Salivary concentrations of 7 suspected periodontal pathogens were analyzed by quantitative real-time PCR, while serum antibody titres were measured by ELISA. In women who had PCOS, salivary populations of
In my search I could not find any reports of associations of the skin, aural, or nasal/sinus biomes with PCOS.
Although newer technologies e.g., 16S rRNA are a giant step forward in our understanding of biome/systemic disorder interactions, it is important to understand that the study of biomes is still in its infancy. Our microbiomes include viruses, fungi, prions, protozoa, and sometimes parasites, and algae. Future research will doubtless uncover important associations between these organisms/pre-organisms and systemic disorders like PCOS.
Endocrine disrupting chemicals (EDCs), both environmental and drug, appear to contribute to the etiopathogenesis of PCOS. This may occur via binding to sex hormone receptors or by causing IR/hyperinsulinemia; additional mechanisms are also possible.
Environmental EDCs-In our species increased serum bisphenol A (BPA) concentrations have been reported in teenagers and women with PCOS compared with reproductively healthy controls and these are positively correlated with androgen levels, suggesting a role for this chemical in the etiopathogenesis of PCOS, although causality is yet be established [56, 57, 58, 59, 60]. It is possible that embryonic/fetal exposure to certain EDCs permanently changes reproductive, neuro-endocrine, and metabolic regulation favoring PCOS development, in genetically predisposed people, or hastening and/or exacerbating the natural course of the disorder via lifelong exposure.
In pre-clinical studies, exposure of mothers to BPA changes postnatal development and sexual maturation in the offspring. Exposure to dibutyl phthalate and di(2-ethylhexyl)phthalate during pregnancy results in polycystic ovaries and a hormonal profile similar to that seen in human PCOS. Androgenic EDCs, nicotine, and 3,4,4′-trichlorocarbanilide, all contribute to the creation of a concerning hyperandrogenic embryonic/fetal milieu. Prenatal EDC exposure may contribute to abnormal embryonic/fetal developmental programming and partially explain the wide variability in PCOS phenotype.
Research has mostly focused on the possible roles of the most widely distributed and studied environmental agents suspected of contributing to the etiopathogenesis of PCOS. Plasticizers, including BPA and phthalates, which are known EDCs, and advanced glycation end products (AGEs) are ubiquitous in our milieu; therefore, our attention should be focused on reducing such exposure. The timing of EDC exposure is critical for understanding the diversity and severity of adverse health consequences. Embryos/fetuses, infants, and young children are the most vulnerable groups. Prenatal EDC exposure that imitates some actions of endogenous hormones may contribute to abnormal fetal programming and, ultimately, result in PCOS and other adverse health consequences, possibly even trans-generationally. Acute or more protracted EDC exposure and dietary (mostly from Western type diets), as well as endogenously formed AGE exposure in different stages of the life cycle can alter the hormonal milieu and result in disruption of reproductive function. AGEs are proinflammatory molecules capable of interacting with cell membrane receptors and mediate triggering of proinflammatory signaling pathways and oxidative stress. These agents may also contribute to metabolic changes, e.g., obesity, IR, and the compensatory hyperinsulinemia that can create or worsen the PCOS phenotype and contribute to its complications, e.g., Type 2 diabetes and cardiovascular disease. Prediabetes and T2DM both result in hyperglycemia, leading to the formation of even more AGEs in a vicious cycle [61, 62].
Large population surveys find countless chemicals in our serum and tissues that did not even exist in our grandparents’ generation [60] Sadly, regulatory agencies are losing the race to evaluate these compounds for safety before they are released into our environment.
In addition to the EDCs which accidentally find their way into our bodies, many prescription drugs may also contribute to the etiopathogenesis and maintenance of PCOS [61]. Most of the drugs which contribute to causing PCOS do so by causing IR/hyperinsulinemia. In so doing they often contribute to causing other disorders associated with IR, including metabolic syndrome, T2DM, hypertension, gout, dyslipidemia, and congenital adrenal hyperplasia [62, 63]. Among these drugs are some of the beta-blockers, thiazides and related diuretics, like indapamide, some of the inhibitors of the renin-angiotensin system, nicotinic acid, the fluoroquinolones (which may also contribute by causing bacterial dysbiosis), protease inhibitors, nucleoside reverse transcriptase inhibitors, antipsychotic drugs, especially atypical antipsychotic drugs, divalproex, and high estrogen oral contraceptives.
The role of vitamin D and polymorphisms in its receptor have been the subject of considerable research, given that vitamin D deficiency has been associated with IR [63, 64, 65, 66, 67, 68]. Vitamin D has a physiologic role in female reproduction, which includes ovarian follicle development and luteinization, by regulating anti-Mullerian hormone (AMH) signaling, follicle-stimulating hormone (FSH) sensitivity, and progesterone biosynthesis in granulosa cells. Vitamin D also affects glucose homeostasis via diverse routes. The evidence for an important role for vitamin D on glucose metabolism includes: the presence of vitamin D receptors in pancreatic β-cells and skeletal muscle, the expression of 1-α-hydroxylase enzyme in these tissues which catalyzes the 1-α-hydroxylation of 25-hydroxy vitamin D (25(OH)D) to 1,25-dihydroxyvitamin D, as well as the presence of a vitamin D response element in the human insulin gene promoter region. About 67–85% of women with PCOS have vitamin D deficiency. While there is no significant difference in serum 25(OH)D concentrations between women with PCOS and controls, a high prevalence of vitamin D deficiency is reported to be associated with metabolic syndrome.
Hypovitaminosis D may worsen the signs and symptoms of PCOS, such as IR, ovulatory and menstrual perturbations, infertility, androgen excess, obesity and increased risk of cardiovascular disease. Many observational reports support a role for vitamin D in an inverse association between women’s vitamin D status and metabolic disturbances in PCOS, however, it is difficult to reach a conclusion regarding causality because of contradictory findings from various individual studies and from a recent meta-analysis.
Supplementation of vitamin D reduces abnormally elevated serum AMH concentrations and raises serum anti-inflammatory soluble receptor for AGEs in women with both vitamin D-deficiency women and PCOS. Notably, vitamin D and calcium added to metformin in women with PCOS and vitamin D deficiency improves menstrual regularity and ovulatory rate.
Low serum 25(OH)D concentrations are significantly associated with IR in women with PCOS, leading to suggestions that genes regulating vitamin D metabolism could be candidate genes for PCOS susceptibility. Certain polymorphisms in the vitamin D receptor (VDR) gene including: Cdx2, Taq1, Bsm1, Apa1, and Fok1, have been reported to play an important regulatory role on insulin secretion and sensitivity in women with PCOS. The VDR Fok1 polymorphism was found to have a protective effect against the risk of Type 2 diabetes mellitus, while the Bsm1 polymorphism augmented the risk of Type 2 diabetes. The Apa1 polymorphism has been reported to reduce the risk of vitamin D deficiency [65].
A study was carried out in India, to investigate the association pattern of 4 VDR polymorphisms (Cdx2, Fok1, Apa1 and Taq1) with PCOS among Indian women. They reported a significant difference in genotype and allele frequency distributions of the Cdx2 polymorphism between women with PCOS and controls. A significantly higher frequency of the heterozygous GA genotype and the A allele of Cdx2 was encountered in control women when compared to those with PCOS (P < 0.001), suggesting that this single nucleotide polymorphism (SNP) affords some protection against PCOS development. Following adjustment for the covariates of BMI and age, the carriers of the GA genotype and the A allele remained relatively protected against PCOS development. No other significant associations were encountered between the remaining 3 VDR polymorphisms (Fok1, Apa1 and Taq1) and PCOS. They also investigated associations between VDR genotypes and some PCOS clinical/biochemical characteristics and reported that the Cdx2 genotypes were significantly associated with serum testosterone levels while the Fok1 polymorphism showed a significant association with infertility. In addition, the 2 haplotypes made up of 4 polymorphisms, ACCA and ACTA, were also significantly associated with PCOS risk [64].
In a group of Austrian women with PCOS, the VDR Cdx2 polymorphism was found to be associated with higher insulin sensitivity, and the Apa1 polymorphism was associated with lower serum testosterone concentrations. Nevertheless, other investigators did not report any significant differences in the VDR gene polymorphism frequencies between women with PCOS and controls [65].
In a study from Taiwan, it was found that the VDR 1a promoter polymorphisms were not associated with the risk of PCOS but were associated with serum 25(OH)D levels. This study also found that significantly lower serum 25(OH)D levels were seen in women who carried the heterozygous 1521CG/1012GA haplotype of the VDR 1a promoter polymorphisms in both women with PCOS and controls. However, metformin was only able to increase serum 25(OH)D concentrations in women with PCOS who carried the homozygous 1521G/1012A haplotype [65].
Even though several polymorphisms in the VDR gene have been implicated in the etiopathogenesis and presenting phenotype of PCOS, there is considerable heterogeneity in reports from both individual investigators and meta-analyses. Therefore, the role of these VDR gene polymorphisms in the pathogenesis of IR and PCOS remains controversial [65].
Future research with large, independent cohorts and with diverse ethnic populations may clarify whether the associations between vitamin D and PCOS are ethnicity-specific or have differing thresholds depending upon the influence of other individual genotypes in women with PCOS.
A recent reanalysis of data from the D2d trial by the original study authors, using a Cox proportional hazards model, concluded that daily vitamin D intake, sufficient to achieve and maintain a serum 25-(OHD) level ≥ 100 nmol/l, is a promising approach to reduce the risk of T2DM in adults with prediabetes, in contrast with their original conclusion, that vitamin D administration was not effective in the prevention of T2DM in those with prediabetes [67, 68].
In addition to the Type B syndrome of severe insulin resistance, acanthosis, SLE with nephritis, & PCOS discussed in the Introduction, several other autoimmune disorders are associated with PCOS [69, 70]. These include vitiligo, alopecia areata, and the autoimmune polyglandular syndrome. Autoimmune thyroid disease, especially autoimmune (Hashimoto’s) thyroiditis, is about 3x more common in women with PCOS compared with controls [70]. Among the reasons cited for these associations are the sustained high estrogen/progesterone ratios in women with PCOS, which prenatally derail embryonic/fetal thymic development and disrupt thymic function as regards preservation of immune self-tolerance, vitamin D deficiency/insufficiency and VDR gene polymorphisms, as well as similarities in the gut biome in people with PCOS and autoimmune disorders, such as increase in those species causing more gut permeability and a reduction of overall bacterial species diversity. These biomic changes are also seen in obesity, metabolic syndrome, and T2DM. In addition, 3 genetic polymorphisms have been reported as predisposing to both PCOS and Hashimoto’s thyroiditis. They are polymorphisms of the genes for gonadotropin releasing hormone receptor, fibrillin 3, regulating the activity of transforming growth factor-β and regulatory T cell levels, and CYP1B1 affecting estradiol hydroxylation.
Murray and colleagues reported PCOS in association with an insulinoma, which resolved following successful removal of the tumor [71]. My literature search did not find any reports of nesidioblastosis-associated PCOS, however, it is predictable, given their chronic hyperinsulinemia, that such individuals will eventually be found.
While there is evident insulin resistance in people with PCOS in terms of carbohydrate, lipid, and uric acid metabolism, there is also evidence of normal or even increased insulin action in features such as hyperandrogenism, acanthosis nigricans, acrochordons, organomegaly, and visceral obesity.
There are 2 major signaling pathways through which insulin’s actions are expressed: one signaling cascade is used to regulate intermediary metabolism while the other modulates growth and cell division as well as the hypothalamic/pituitary, gonadal and adrenocortical axes. Regulation of these 2 distinct cascades may be dissociated and data suggest that the activity of the signaling pathway which governs intermediary metabolism is decreased in people with PCOS, T2DM, metabolic syndrome, gout, and congenital adrenal hyperplasia, while the pathways modulating growth processes and mitoses is normal or even enhanced [72]. Most of the intermediary metabolism pathway is activated by insulin binding to its own receptor followed by phosphorylation of IRS-1 and IRS-2. Some of the pathway regulating growth and cell division is initiated by insulin binding to IGF-1 receptors. Even though insulin has greater affinity for its own receptor, when insulin levels are high its receptor is downregulated, limiting available binding sites, so that “excess” insulin will bind to the IGF-1 receptor as an agonist, mimicking the effects of growth hormone. When activation of the IGF-1 cascade is extreme it is sometimes referred to as pseudo acromegaly [73]. Studies show that insulin’s signaling pathways normally regulate cell growth, metabolism and survival via activation of mitogen-activated protein kinases (MAPKs) and phosphotidylinositide-3-kinase (PI3K). Activation of PI-3K-associated with insulin receptor substrates-1 and -2 (IRS1, 2) and the subsequent Akt → Foxo1 phosphorylation cascade plays a pivotal role in regulating nutrient homeostasis and organ survival. Several mechanisms have been suggested as causes contributing to the development of IR and metabolic syndrome. These include genetic polymorphisms of proteins in the insulin signaling cascade, suboptimal fetal nutrition, and increased intra-abdominal fat. IR develops as the key player in a cluster of cardiovascular/metabolic dysfunctions we now recognize as metabolic syndrome, which may result in T2DM, a distinctive (Type IV, Fredrickson) dyslipidemia with high VLDL, low HDL, and normal-moderately elevated LDL, accelerated atherosclerosis, hypertension, or congenital adrenal hyperplasia depending on the genetic/epigenetic background of the person with IR including the genetic/epigenetic characteristics of our relevant biomes, vitamin D status, and the influence of drugs and environmental chemicals with endocrine disruptor effects. Inactivation of Akt and activation of Foxo1, via suppression of IRS1 and IRS2 in different tissues following hyperinsulinemia, metabolic inflammation, and overnutrition could be the mechanisms leading to metabolic syndrome in our species [74].
IR in women with PCOS seems to be associated with exaggerated serine residue phosphorylation of insulin receptor substrates. An enzyme extrinsic to the insulin receptors, quite possibly a serine/threonine kinase, causes this aberration and exemplifies a key mechanism for induction of human IR related to extrinsic factors regulating insulin receptor signaling. Serine phosphorylation seems to regulate the activity of P450c16, the pivotal regulatory enzyme in androgen biosynthesis. It is very possible that a single defect results in both IR and hyperandrogenism in some women with PCOS. This IR is selective, affecting glucose/lipid metabolism, but not cell division or growth [75].
It has been reported that women with PCOS have significantly higher risk of obstructive sleep apnea (OSA). OSA severity is significantly correlated with plasma glucose and insulin levels and homeostasis model assessment for insulin resistance (HOMA-IR)-index in women with PCOS. It appears that the progressive worsening of PCOS results in OSA which, in turn, exacerbates the metabolic disturbances, such as IR, associated with this syndrome [76].
Clinic-based studies report that sleep disturbance and disorders such as OSA and excessive daytime sleepiness are more frequently encountered among women with PCOS. Data from the few published population-based studies is substantially concordant. Women with PCOS are mostly overweight/obese, however, this fact only partially explains their sleep problems as significant associations persist after adjusting for body mass index; sleep issues also occur in lean women with PCOS. There are several, likely bidirectional, pathways through which PCOS and sleep disturbances are associated. PCOS pathophysiology includes hyperandrogenemia, a unique form of IR, and possible changes in cortisol and melatonin secretion, plausibly reflecting hypothalamic-pituitary-adrenal dysfunction. Psychological/behavioral factors probably also play a role, such as anxiety and depression, tobacco use, alcohol use, and insufficient exercise which are also frequent among women with PCOS, likely in response to their symptoms. The effects of sleep disturbances on the health of women with PCOS is not completely understood, however, both PCOS and disordered sleep are associated with worsening long term cardiometabolic health and augmented T2DM risk. Immediate quality of life and long-term health status of women with PCOS will likely improve from timely diagnosis and comprehensive management of sleep disorders [77].
Several investigators have reported that exposure of rats to continuous light can induce PCOS; however, hyperandrogenism, a key feature of human PCOS, has not been reported previously. In Kang et al.’s article they reported that (a) body weight declined in female rats in continuous light conditions with both ovarian and uterine augmentation; (b) the estrous cycle in rats living in continuous light was disordered, and PCOS-like changes were noted accompanied by hair loss and lethargy; and (c) serum testosterone levels rose significantly in rats living in continuous light. Their results suggest that continuous light can lead to PCOS in female rats without the need for drugs. Poor sleep habits, faulty sleep hygiene, and light pollution may be important contributors to the pathogenesis of PCOS [78].
Dominoni and colleagues as well as others have described reproductive hardships in free-living wildlife associated with light pollution [79].
In addition, human-generated noise pollution has been implicated in reduced reproductive success in wildlife, although the mechanisms involved are not clear [80].
Based on my years in clinical practice and academia, I hope readers will indulge me in a personal gripe. When applying the Rotterdam criteria for the diagnosis of PCOS many clinicians ignore or only pay lip service to the exclusions which must be considered an essential part of these criteria. These include thyroid disease, Cushing’s syndrome, androgen-secreting neoplasia, hyperprolactinemia, and non-classical congenital adrenal hyperplasia. In my referral practice I found, in reviewing the referral or the written or electronic medical records of patients referred to me for PCOS treatment, that these conditions, especially NCAH had very seldom been excluded by the referring colleague. In the PCOS research literature many investigators do not mention exclusion of these disorders in their PCOS cohorts. In many other articles a single morning unstimulated serum 17-OH-progesterone is proffered as excluding NCAH. The best articles offer a cosyntropin-stimulated 17-OH-progesterone to exclude this diagnosis. In my readings I have not yet encountered a study where NCAH was thoroughly excluded with genetic testing for 21-hydroxylase deficiency as well as cosyntropin stimulation of 17-OH-progesterone, 17-OH-pregnenolone, 11-deoxycortisol, deoxycorticosterone, corticosterone, and 18-OH-corticosterone. Thus, without fully testing for NCAH, most of us have the impression that PCOS is very common and NCAH, except in high-risk ethnic groups is very rare. This is concerning because NCAH and PCOS are often phenotypically identical. However, since therapies aimed at decreasing IR, normalizing the menstrual cycle, reducing androgen secretion or expression, and inducing ovulation are often able to ameliorate both conditions the real-world consequences of misdiagnosis of PCOS may not be as grave as we might expect [63]. Carbunaru and colleagues have reported that the common, non-classic or phenotypic form of 3-beta-ol dehydrogenase deficiency (3-beta-HSD) controlling the adrenal/ovarian synthesis of this enzyme is not associated with an exonic polymorphism, but is associated with IR, hyperandrogenism, and a PCOS phenotype, which in severe forms is called Hyperandrogenism, Insulin Resistance-Acanthosis Nigricans (HAIR-AN) syndrome [81]. It is possible, that a polymorphism may exist in the promoter region of the gene, as has been reported in a group of Brazilian women with non-classic 21-hydroxylase deficiency [82]. Alternatively, several epigenetic modifications could be downregulating the expression of the gene.
Lipodystrophies are associated with PCOS due to insulin resistance, which is intrinsic to the lipodystrophies [83, 84].
In this chapter I have tried to highlight truly rare contributing causes of PCOS, like insulinomas, as well as showcasing causes that are not particularly rare, but are very rarely considered in clinical practice. The latter include: biomic alterations, epigenetic disturbances such as disordered DNA methylation and/or histone acetylation, and EDCs, including many drugs which contribute to IR. In addition, I have described some very rarely reported causes, like cysticercosis, which, given its extensive global endemicity, will likely turn out to be much more common causes of PCOS than is currently recognized. In exploring this topic, I hope that I have shed some light on common pathways by which these diverse agents might contribute to the etiopathogenesis and maintenance of PCOS, mostly by causing IR/hyperinsulinemia, hyperandrogenism, chronic inflammation, or unopposed estrogenic effects. It is hoped that clinicians will consider these causes more often when evaluating their patients and considering treatments. In so doing, it is likely that better treatment results can be achieved. It is already possible for individual clinicians and their patients to achieve much with interventions such as lower calorie, plant-based diets, supplementation with pre- and probiotics, exercise, ensuring adequate vitamin D status, and choosing drugs with favorable effects on the biome. In addition, patients once educated, may be able to improve their therapeutic outcomes by minimizing their exposure to EDCs in plastics, self-care products, and household products. Major improvements in outcomes may result from efforts at the community, regional, national, and international levels to improve diets, increase exercise, and reduce our exposure to EDCs, light and noise pollution. Attention to sleep hygiene by patients and providers may further reduce the burden of PCOS, metabolic syndrome, resistant hypertension, and T2DM. Fecal transplantation may jump start amelioration of PCOS, provided it is followed with sustained lifestyle changes including plant-based diets, exercise, and possibly pre- and probiotic supplementation. Looking toward the future, the experience we have gained in developing mRNA vaccines against COVID-19 might be applied to develop mRNA “vaccines” against gene products whose overabundance is contributing to PCOS. A fragment of the mRNA could be used to synthesize a fragment of the peptide different enough from the native protein to provoke an adaptive immune response.
Our understanding of the biome and of epigenetics is still in its infancy. As more is learned the opportunities for precision prevention and treatment will increase.
The author declares no conflict of interest.
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Due to its advantages of abundant resources, less in cost, great workability and high physical properties, fly ash leads to achieving high mechanical properties. Fly ash is considered as one of the largest generated industrial solid wastes or so-called industrial by-products, around the world particularly in China, India, and USA. The characteristics of fly ash allow it to be a geotechnical material to produce geopolymer cement or concrete as an alternative of ordinary Portland cement. Many efforts are made in this direction to formulate a suitable mix design of fly ash-based geopolymer by focusing on fly ash as the main prime material. The physical properties, chemical compositions, and chemical activation of fly ash are analyzed and evaluated in this review paper. Reference has been made to different ASTM, ACI standards, and other researches work in geopolymer area.",book:{id:"9916",slug:"zero-energy-buildings-new-approaches-and-technologies",title:"Zero-Energy Buildings",fullTitle:"Zero-Energy Buildings - New Approaches and Technologies"},signatures:"Aissa Bouaissi, Long Yuan Li, Mohd Mustafa Al Bakri Abdullah, Romisuhani Ahmad, Rafiza Abdul Razak and Zarina Yahya",authors:null},{id:"73729",doi:"10.5772/intechopen.93500",title:"Solar Energy and Its Purpose in Net-Zero Energy Building",slug:"solar-energy-and-its-purpose-in-net-zero-energy-building",totalDownloads:603,totalCrossrefCites:3,totalDimensionsCites:5,abstract:"The Net Zero Energy Building is generally described as an extremely energy-efficient building in which the residual electricity demand is provided by renewable energy. Solar power is also regarded to be the most readily available and usable form of renewable electricity produced at the building site. In contrast, energy conservation is viewed as an influential national for achieving a building’s net zero energy status. This chapter aims to show the value of the synergy between energy conservation and solar energy transfer to NZEBs at the global and regional levels. To achieve these goals, both energy demand building and the potential supply of solar energy in buildings have been forecasted in various regions, climatic conditions, and types of buildings. Building energy consumption was evaluated based on a bottom-up energy model developed by 3CSEP and data inputs from the Bottom-Up Energy Analysis System (BUENAS) model under two scenarios of differing degrees of energy efficiency intention. The study results indicate that the acquisition of sustainable energy consumption is critical for solar-powered net zero energy buildings in various building styles and environments. The chapter calls for the value of government measures that incorporate energy conservation and renewable energy.",book:{id:"9916",slug:"zero-energy-buildings-new-approaches-and-technologies",title:"Zero-Energy Buildings",fullTitle:"Zero-Energy Buildings - New Approaches and Technologies"},signatures:"Mostafa Esmaeili Shayan",authors:[{id:"317852",title:"Ph.D.",name:"Mostafa",middleName:null,surname:"Esmaeili Shayan",slug:"mostafa-esmaeili-shayan",fullName:"Mostafa Esmaeili Shayan"}]},{id:"67105",doi:"10.5772/intechopen.86279",title:"Social Innovation and Environmental Sustainability in Social Housing Policies: Learning from Two Experimental Case Studies in Italy",slug:"social-innovation-and-environmental-sustainability-in-social-housing-policies-learning-from-two-expe",totalDownloads:1011,totalCrossrefCites:2,totalDimensionsCites:4,abstract:"This chapter critically examines approaches and solutions developed by social housing to sustainably respond to the housing emergency plaguing contemporary cities and Italian cities in particular. In a broader perspective, we also investigate how housing has become ‘difficult’ in Europe and the poorest segments of the population run the risk of having their right to housing dramatically denied. Analysing housing in terms of its procedural dimension, we focus on two Italian case studies that evoke a new way of inhabiting the city, cases in which high standards characterised social housing and yet remain accessible to all. The Sharing hotel residence in Turin and Zoia social housing in Milan combine housing with other socially innovative measures in a framework of sustainability and avant-garde construction. These are significant examples that speak to issues such as temporariness, flexibility and the coordination of measures. These two cases both pursued objectives having to do with social, planning, architectural and environmental quality, albeit each in their own way. There are by now numerous examples of social housing in Europe and these have recently attracted growing interest in Italy as well; in this country, however, such projects represent valid instances of experimentation but are not at all widespread.",book:{id:"7650",slug:"different-strategies-of-housing-design",title:"Different Strategies of Housing Design",fullTitle:"Different Strategies of Housing Design"},signatures:"Rossana Galdini and Silvia Lucciarini",authors:[{id:"281246",title:"Dr.",name:"Silvia",middleName:null,surname:"Lucciarini",slug:"silvia-lucciarini",fullName:"Silvia Lucciarini"},{id:"282958",title:"Prof.",name:"Rossana",middleName:null,surname:"Galdini",slug:"rossana-galdini",fullName:"Rossana Galdini"}]},{id:"67084",doi:"10.5772/intechopen.86278",title:"Comprehensive Strategy for Sustainable Housing Design",slug:"comprehensive-strategy-for-sustainable-housing-design",totalDownloads:1362,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Sustainable housing needs to be designed to maximize occupants’ well-being and minimize the environmental load. The pursuit of combining these two different aspects toward sustainability is a goal-oriented task. The science of control can be applied to all goal-oriented tasks. Therefore, applying control science, we have been progressing in research on sustainable housing design. Our previous study has produced the control system for promoting sustainable housing design in which sustainable design guidelines and sustainability checklist are incorporated. Based on these accomplished results, this study has comprehensively visualized the process of producing and revising the sustainable design guidelines and sustainability checklist. Following this visualized process, also this study has concretely shown the production and revision processes of the sustainable design guidelines. The study results suggest that the comprehensive visualization can make these processes more manageable and help system designers to produce and revise the guidelines more efficiently. Furthermore, these results have led to indicating how to adjust the guidelines to different countries or regions as well as changing situations over time.",book:{id:"7650",slug:"different-strategies-of-housing-design",title:"Different Strategies of Housing Design",fullTitle:"Different Strategies of Housing Design"},signatures:"Kazutoshi Fujihira",authors:[{id:"69662",title:"BSc.",name:"Kazutoshi",middleName:null,surname:"Fujihira",slug:"kazutoshi-fujihira",fullName:"Kazutoshi Fujihira"}]},{id:"57401",doi:"10.5772/intechopen.71325",title:"Basic Schemes: Preparations for Applying Control Science to Sustainable Design",slug:"basic-schemes-preparations-for-applying-control-science-to-sustainable-design",totalDownloads:1223,totalCrossrefCites:3,totalDimensionsCites:3,abstract:"It is the ultimate goal for humankind to deal with various problems and achieve sustainability. Control science can be applied to all goal-oriented tasks and has already produced remarkable results. Accordingly, applying control science to the task of achieving sustainability is a rational and reliable approach. In order to apply control science to sustainability issues, our first study has shown the “basic control system for sustainability” as well as the “model of sustainability.” After that, in order to identify system components of practical control systems for promoting sustainable design, we have devised “two-step preparatory work for sustainable design.” The two steps of this preparatory work are “determining the relationships between the standard human activities and sustainability” and “sustainability checkup on human activities as an object.”",book:{id:"5692",slug:"sustainable-home-design-by-applying-control-science",title:"Sustainable Home Design by Applying Control Science",fullTitle:"Sustainable Home Design by Applying Control Science"},signatures:"Kazutoshi Fujihira",authors:[{id:"69662",title:"BSc.",name:"Kazutoshi",middleName:null,surname:"Fujihira",slug:"kazutoshi-fujihira",fullName:"Kazutoshi Fujihira"}]}],mostDownloadedChaptersLast30Days:[{id:"71982",title:"Net-Zero Energy Buildings: Principles and Applications",slug:"net-zero-energy-buildings-principles-and-applications",totalDownloads:2226,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Global warming and climate change are rising issues during the last couple of decades. With residential and commercial buildings being the largest energy consumers, sources are being depleted at a much faster pace in the recent decades. Recent statistics shows that 14% of humans are active participant to protect the environment with an additional 48% sympathetic but not active. In this chapter, net-zero energy buildings design tools and applications are presented that can help designers in the commercial and residential sectors design their buildings to be net-zero energy buildings. Case studies with benefits and challenges will be presented to illustrate the different designs to achieve a net-zero energy building (NZEB).",book:{id:"9916",slug:"zero-energy-buildings-new-approaches-and-technologies",title:"Zero-Energy Buildings",fullTitle:"Zero-Energy Buildings - New Approaches and Technologies"},signatures:"Maher Shehadi",authors:null},{id:"57400",title:"Case Study: Detached House Designed by Following the Control System",slug:"case-study-detached-house-designed-by-following-the-control-system",totalDownloads:1548,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"The previous chapter has demonstrated the control system for promoting sustainable housing design in which the sustainable design guidelines and sustainability checklist are incorporated. Following this control system, we have actually designed and constructed a detached house. To be concrete, the homeowner and the architects of the housing manufacture have designed the home’s parts, or elements, so that as much as possible the elements’ variables meet their desired values. The sustainable design guidelines and sustainability checklist have been readily accepted because the material and spatial elements are equivalent to real parts of the home. After the home started to be used, we have obtained external evaluations of the home’s sustainability performance. For example, CASBEE for Detached Houses, a comprehensive assessment system, has readily ranked the house in the highest “S.” An energy-saving performance assessment has shown that this home has reduced energy consumption by over 70%, as compared with the average home. On the other hand, the reactions of the occupants and visitors have indicated the comfort, healthiness and safety of this house. Furthermore, this home has received a sustainable housing award, especially due to its extremely high sustainability and energy-saving performance.",book:{id:"5692",slug:"sustainable-home-design-by-applying-control-science",title:"Sustainable Home Design by Applying Control Science",fullTitle:"Sustainable Home Design by Applying Control Science"},signatures:"Kazutoshi Fujihira",authors:[{id:"69662",title:"BSc.",name:"Kazutoshi",middleName:null,surname:"Fujihira",slug:"kazutoshi-fujihira",fullName:"Kazutoshi Fujihira"}]},{id:"67084",title:"Comprehensive Strategy for Sustainable Housing Design",slug:"comprehensive-strategy-for-sustainable-housing-design",totalDownloads:1362,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Sustainable housing needs to be designed to maximize occupants’ well-being and minimize the environmental load. The pursuit of combining these two different aspects toward sustainability is a goal-oriented task. The science of control can be applied to all goal-oriented tasks. Therefore, applying control science, we have been progressing in research on sustainable housing design. Our previous study has produced the control system for promoting sustainable housing design in which sustainable design guidelines and sustainability checklist are incorporated. Based on these accomplished results, this study has comprehensively visualized the process of producing and revising the sustainable design guidelines and sustainability checklist. Following this visualized process, also this study has concretely shown the production and revision processes of the sustainable design guidelines. The study results suggest that the comprehensive visualization can make these processes more manageable and help system designers to produce and revise the guidelines more efficiently. Furthermore, these results have led to indicating how to adjust the guidelines to different countries or regions as well as changing situations over time.",book:{id:"7650",slug:"different-strategies-of-housing-design",title:"Different Strategies of Housing Design",fullTitle:"Different Strategies of Housing Design"},signatures:"Kazutoshi Fujihira",authors:[{id:"69662",title:"BSc.",name:"Kazutoshi",middleName:null,surname:"Fujihira",slug:"kazutoshi-fujihira",fullName:"Kazutoshi Fujihira"}]},{id:"65804",title:"Effects of Street Geometry on Airflow Regimes for Natural Ventilation in Three Different Street Configurations in Enugu City",slug:"effects-of-street-geometry-on-airflow-regimes-for-natural-ventilation-in-three-different-street-conf",totalDownloads:1401,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"Efficient natural ventilation is dependent on the micro climate conditions of an urban environment. This is affected by ambient wind flow, radiation and air temperatures. The airflow within the urban street can be cultivated into two regions. The first is a recirculation region, which forms in the near wake of each building. The Second is a ventilated region downstream of the recirculation region, formed when the street is sufficiently wide. The development of the flow into these two regions depends on geometry. This chapter looks at the impacts of street geometry on these regions of airflow cultivation in three different street configurations in high density residential settlements in Enugu city. It utilized schematic analysis of airflow regimes to identify the behaviors of flow in these street configurations relative to the height and width ratios of the street canyon. This schematic analysis can be utilized in preliminary design studies by city and building designers for justifying street dimensions and configurations in tropical regions where natural ventilation is paramount.",book:{id:"7650",slug:"different-strategies-of-housing-design",title:"Different Strategies of Housing Design",fullTitle:"Different Strategies of Housing Design"},signatures:"Jideofor Anselm Akubue",authors:[{id:"139659",title:"Dr.",name:"Akubue",middleName:"Jideofor",surname:"Anselm",slug:"akubue-anselm",fullName:"Akubue Anselm"}]},{id:"66000",title:"Fundamentals of Natural Ventilation Design within Dwellings",slug:"fundamentals-of-natural-ventilation-design-within-dwellings",totalDownloads:962,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Along with acoustical and lighting comfort, indoor air quality (IAQ) and thermal comfort upon households are essential to maintain a proper indoor environment, therefore ensuring a welfare toward the occupants. Nevertheless, sometimes, these features are neglected by building designers and constructers, causing problems such as the so-called sick building syndrome (SBS) and thermal discomfort, among others. Although there are short-term solutions such as purifiers, extractors, fans, and air conditioning, eventually these methods become not sustainable activities that consume energy and emit polluting gases such as chlorofluorocarbons. One alternative to this is natural ventilation, understood as the airflow throughout a building caused by changes of pressures naturally produced. In this chapter, the role of the early-stage building design as well as the correct occupant behavior is presented as essential to develop a naturally ventilated dwelling, which is an excellent alternative to achieve proper levels of indoor environment in a sustainable manner.",book:{id:"7650",slug:"different-strategies-of-housing-design",title:"Different Strategies of Housing Design",fullTitle:"Different Strategies of Housing Design"},signatures:"Ivan Oropeza-Perez",authors:[{id:"282172",title:"Dr.",name:"Ivan",middleName:null,surname:"Oropeza-Perez",slug:"ivan-oropeza-perez",fullName:"Ivan Oropeza-Perez"}]}],onlineFirstChaptersFilter:{topicId:"852",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"6",title:"Infectious Diseases",doi:"10.5772/intechopen.71852",issn:"2631-6188",scope:"This series will provide a comprehensive overview of recent research trends in various Infectious Diseases (as per the most recent Baltimore classification). Topics will include general overviews of infections, immunopathology, diagnosis, treatment, epidemiology, etiology, and current clinical recommendations for managing infectious diseases. Ongoing issues, recent advances, and future diagnostic approaches and therapeutic strategies will also be discussed. This book series will focus on various aspects and properties of infectious diseases whose deep understanding is essential for safeguarding the human race from losing resources and economies due to pathogens.",coverUrl:"https://cdn.intechopen.com/series/covers/6.jpg",latestPublicationDate:"June 25th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:13,editor:{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. 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Completed the Course Medical Mycology, the Centraalbureau voor Schimmelcultures (CBS), Fungal Biodiversity Centre, Netherlands (2006). International Union of Microbiological Societies (IUMS) Fellow, and International Emerging Infectious Diseases (IEID) Fellow, Centers for Diseases Control and Prevention (CDC), Atlanta, USA. Diploma of Dermatological Scientist, Japanese Society for Investigative Dermatology. Ph.D. of Juntendo University, Japan. Bachelor’s and Master’s degree, Medicine, West China University of Medical Sciences. Chair of Sichuan Medical Association Dermatology Committee. General Secretary of The 19th Annual Meeting of Chinese Society of Dermatology and the Asia Pacific Society for Medical Mycology (2013). In charge of the Annual Medical Mycology Course over 20-years authorized by National Continue Medical Education Committee of China. Member of the board of directors of the Asia-Pacific Society for Medical Mycology (APSMM). Associate editor of Mycopathologia. Vice-chief of the editorial board of Chinses Journal of Mycology, China. Board Member and Chair of Mycology Group of Chinese Society of Dermatology.",institutionString:null,institution:{name:"Sichuan University",institutionURL:null,country:{name:"China"}}},editorTwo:null,editorThree:null},{id:"5",title:"Parasitic Infectious Diseases",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",isOpenForSubmission:!0,editor:{id:"67907",title:"Dr.",name:"Amidou",middleName:null,surname:"Samie",slug:"amidou-samie",fullName:"Amidou Samie",profilePictureURL:"https://mts.intechopen.com/storage/users/67907/images/system/67907.jpg",biography:"Dr. Amidou Samie is an Associate Professor of Microbiology at the University of Venda, in South Africa, where he graduated for his PhD in May 2008. He joined the Department of Microbiology the same year and has been giving lectures on topics covering parasitology, immunology, molecular biology and industrial microbiology. He is currently a rated researcher by the National Research Foundation of South Africa at category C2. He has published widely in the field of infectious diseases and has overseen several MSc’s and PhDs. His research activities mostly cover topics on infectious diseases from epidemiology to control. His particular interest lies in the study of intestinal protozoan parasites and opportunistic infections among HIV patients as well as the potential impact of childhood diarrhoea on growth and child development. He also conducts research on water-borne diseases and water quality and is involved in the evaluation of point-of-use water treatment technologies using silver and copper nanoparticles in collaboration with the University of Virginia, USA. 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His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}},editorTwo:null,editorThree:null}]},overviewPageOFChapters:{paginationCount:10,paginationItems:[{id:"82380",title:"Evolution of Parasitism and Pathogenic Adaptations in Certain Medically Important Fungi",doi:"10.5772/intechopen.105206",signatures:"Gokul Shankar Sabesan, Ranjit Singh AJA, Ranjith Mehenderkar and Basanta Kumar Mohanty",slug:"evolution-of-parasitism-and-pathogenic-adaptations-in-certain-medically-important-fungi",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Fungal Infectious Diseases - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11400.jpg",subseries:{id:"4",title:"Fungal Infectious Diseases"}}},{id:"82367",title:"Spatial Variation and Factors Associated with Unsuppressed HIV Viral Load among Women in an HIV Hyperendemic Area of KwaZulu-Natal, South Africa",doi:"10.5772/intechopen.105547",signatures:"Adenike O. 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Kharsany, Temesgen Zewotir and Delia North",slug:"spatial-variation-and-factors-associated-with-unsuppressed-hiv-viral-load-among-women-in-an-hiv-hype",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"HIV-AIDS - Updates, Perspectives and Applications",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82193",title:"Enterococcal Infections: Recent Nomenclature and emerging trends",doi:"10.5772/intechopen.104792",signatures:"Kavita Raja",slug:"enterococcal-infections-recent-nomenclature-and-emerging-trends",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}},{id:"82207",title:"Management Strategies in Perinatal HIV",doi:"10.5772/intechopen.105451",signatures:"Kayla Aleshire and Rima Bazzi",slug:"management-strategies-in-perinatal-hiv",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"HIV-AIDS - Updates, Perspectives and Applications",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}}]},overviewPagePublishedBooks:{paginationCount:13,paginationItems:[{type:"book",id:"6667",title:"Influenza",subtitle:"Therapeutics and Challenges",coverURL:"https://cdn.intechopen.com/books/images_new/6667.jpg",slug:"influenza-therapeutics-and-challenges",publishedDate:"September 19th 2018",editedByType:"Edited by",bookSignature:"Shailendra K. 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He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7064",title:"Current Perspectives in Human Papillomavirus",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7064.jpg",slug:"current-perspectives-in-human-papillomavirus",publishedDate:"May 2nd 2019",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"d92a4085627bab25ddc7942fbf44cf05",volumeInSeries:2,fullTitle:"Current Perspectives in Human Papillomavirus",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7123",title:"Current Topics in Neglected Tropical Diseases",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7123.jpg",slug:"current-topics-in-neglected-tropical-diseases",publishedDate:"December 4th 2019",editedByType:"Edited by",bookSignature:"Alfonso J. Rodriguez-Morales",hash:"61c627da05b2ace83056d11357bdf361",volumeInSeries:3,fullTitle:"Current Topics in Neglected Tropical Diseases",editors:[{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. 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He obtained a Master’s degree in Public Health and PhD in Public Health and Epidemiology. He has a background in Clinical Medicine and has taken courses at higher diploma levels in public health from University of Transkei, Republic of South Africa, and African Medical and Research Foundation (AMREF) in Nairobi, Kenya. Dr. Kasenga worked in different places in and outside Malawi, and has held various positions, such as Licensed Medical Officer, HIV/AIDS Programme Officer, HIV/AIDS resource person in the International Department of Diakonhjemet College, Oslo, Norway. He also managed an Integrated HIV/AIDS Prevention programme for over 5 years. He is currently working as a Director for the Health Ministries Department of Malawi Union of the Seventh Day Adventist Church. Dr. Kasenga has published over 5 articles on HIV/AIDS issues focusing on Prevention of Mother to Child Transmission of HIV (PMTCT), including a book chapter on HIV testing counseling (currently in press). 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Ms. Mehtab has published seven papers in international conferences and one of her papers has been accepted for publication in a reputable international journal. She has won the best paper awards in two prestigious international conferences – BAICONF 2019, and ICADCML 2021, organized in the Indian Institute of Management, Bangalore, India in December 2019, and SOA University, Bhubaneswar, India in January 2021. Besides, Ms. Mehtab has also published two book chapters in two books. Seven of her book chapters will be published in a volume shortly in 2021 by Cambridge Scholars’ Press, UK. Currently, she is working as the joint editor of two edited volumes on Time Series Analysis and Forecasting to be published in the first half of 2021 by an international house. Currently, she is working as a Data Scientist with an MNC in Delhi, India.",institutionString:"NSHM College of Management and Technology",institution:null},{id:"226240",title:"Dr.",name:"Andri Irfan",middleName:null,surname:"Rifai",slug:"andri-irfan-rifai",fullName:"Andri Irfan Rifai",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226240/images/7412_n.jpg",biography:"Andri IRFAN is a Senior Lecturer of Civil Engineering and Planning. He completed the PhD at the Universitas Indonesia & Universidade do Minho with Sandwich Program Scholarship from the Directorate General of Higher Education and LPDP scholarship. He has been teaching for more than 19 years and much active to applied his knowledge in the project construction in Indonesia. His research interest ranges from pavement management system to advanced data mining techniques for transportation engineering. He has published more than 50 papers in journals and 2 books.",institutionString:null,institution:{name:"Universitas Internasional Batam",country:{name:"Indonesia"}}},{id:"314576",title:"Dr.",name:"Ibai",middleName:null,surname:"Laña",slug:"ibai-lana",fullName:"Ibai Laña",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314576/images/system/314576.jpg",biography:"Dr. Ibai Laña works at TECNALIA as a data analyst. He received his Ph.D. in Artificial Intelligence from the University of the Basque Country (UPV/EHU), Spain, in 2018. He is currently a senior researcher at TECNALIA. His research interests fall within the intersection of intelligent transportation systems, machine learning, traffic data analysis, and data science. He has dealt with urban traffic forecasting problems, applying machine learning models and evolutionary algorithms. He has experience in origin-destination matrix estimation or point of interest and trajectory detection. Working with large volumes of data has given him a good command of big data processing tools and NoSQL databases. He has also been a visiting scholar at the Knowledge Engineering and Discovery Research Institute, Auckland University of Technology.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"314575",title:"Dr.",name:"Jesus",middleName:null,surname:"L. Lobo",slug:"jesus-l.-lobo",fullName:"Jesus L. Lobo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314575/images/system/314575.png",biography:"Dr. Jesús López is currently based in Bilbao (Spain) working at TECNALIA as Artificial Intelligence Research Scientist. In most cases, a project idea or a new research line needs to be investigated to see if it is good enough to take into production or to focus on it. 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He is also active in popular science writing data science stories for reputed blogs (KDNuggets, TowardsDataScience, Naukas). Besides, he has recently embarked on mentoring programmes as mentor, and has also worked as data science trainer.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"103779",title:"Prof.",name:"Yalcin",middleName:null,surname:"Isler",slug:"yalcin-isler",fullName:"Yalcin Isler",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRyQ8QAK/Profile_Picture_1628834958734",biography:"Yalcin Isler (1971 - Burdur / Turkey) received the B.Sc. degree in the Department of Electrical and Electronics Engineering from Anadolu University, Eskisehir, Turkey, in 1993, the M.Sc. degree from the Department of Electronics and Communication Engineering, Suleyman Demirel University, Isparta, Turkey, in 1996, the Ph.D. degree from the Department of Electrical and Electronics Engineering, Dokuz Eylul University, Izmir, Turkey, in 2009, and the Competence of Associate Professorship from the Turkish Interuniversity Council in 2019.\n\nHe was Lecturer at Burdur Vocational School in Suleyman Demirel University (1993-2000, Burdur / Turkey), Software Engineer (2000-2002, Izmir / Turkey), Research Assistant in Bulent Ecevit University (2002-2003, Zonguldak / Turkey), Research Assistant in Dokuz Eylul University (2003-2010, Izmir / Turkey), Assistant Professor at the Department of Electrical and Electronics Engineering in Bulent Ecevit University (2010-2012, Zonguldak / Turkey), Assistant Professor at the Department of Biomedical Engineering in Izmir Katip Celebi University (2012-2019, Izmir / Turkey). He is an Associate Professor at the Department of Biomedical Engineering at Izmir Katip Celebi University, Izmir / Turkey, since 2019. In addition to academics, he has also founded Islerya Medical and Information Technologies Company, Izmir / Turkey, since 2017.\n\nHis main research interests cover biomedical signal processing, pattern recognition, medical device design, programming, and embedded systems. He has many scientific papers and participated in several projects in these study fields. He was an IEEE Student Member (2009-2011) and IEEE Member (2011-2014) and has been IEEE Senior Member since 2014.",institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"339677",title:"Dr.",name:"Mrinmoy",middleName:null,surname:"Roy",slug:"mrinmoy-roy",fullName:"Mrinmoy Roy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/339677/images/16768_n.jpg",biography:"An accomplished Sales & Marketing professional with 12 years of cross-functional experience in well-known organisations such as CIPLA, LUPIN, GLENMARK, ASTRAZENECA across different segment of Sales & Marketing, International Business, Institutional Business, Product Management, Strategic Marketing of HIV, Oncology, Derma, Respiratory, Anti-Diabetic, Nutraceutical & Stomatological Product Portfolio and Generic as well as Chronic Critical Care Portfolio. A First Class MBA in International Business & Strategic Marketing, B.Pharm, D.Pharm, Google Certified Digital Marketing Professional. Qualified PhD Candidate in Operations and Management with special focus on Artificial Intelligence and Machine Learning adoption, analysis and use in Healthcare, Hospital & Pharma Domain. Seasoned with diverse therapy area of Pharmaceutical Sales & Marketing ranging from generating revenue through generating prescriptions, launching new products, and making them big brands with continuous strategy execution at the Physician and Patients level. Moved from Sales to Marketing and Business Development for 3.5 years in South East Asian Market operating from Manila, Philippines. Came back to India and handled and developed Brands such as Gluconorm, Lupisulin, Supracal, Absolut Woman, Hemozink, Fabiflu (For COVID 19), and many more. In my previous assignment I used to develop and execute strategies on Sales & Marketing, Commercialization & Business Development for Institution and Corporate Hospital Business portfolio of Oncology Therapy Area for AstraZeneca Pharma India Ltd. Being a Research Scholar and Student of ‘Operations Research & Management: Artificial Intelligence’ I published several pioneer research papers and book chapters on the same in Internationally reputed journals and Books indexed in Scopus, Springer and Ei Compendex, Google Scholar etc. Currently, I am launching PGDM Pharmaceutical Management Program in IIHMR Bangalore and spearheading the course curriculum and structure of the same. I am interested in Collaboration for Healthcare Innovation, Pharma AI Innovation, Future trend in Marketing and Management with incubation on Healthcare, Healthcare IT startups, AI-ML Modelling and Healthcare Algorithm based training module development. I am also an affiliated member of the Institute of Management Consultant of India, looking forward to Healthcare, Healthcare IT and Innovation, Pharma and Hospital Management Consulting works.",institutionString:null,institution:{name:"Lovely Professional University",country:{name:"India"}}},{id:"310576",title:"Prof.",name:"Erick Giovani",middleName:null,surname:"Sperandio Nascimento",slug:"erick-giovani-sperandio-nascimento",fullName:"Erick Giovani Sperandio Nascimento",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y00002pDKxDQAW/ProfilePicture%202022-06-20%2019%3A57%3A24.788",biography:"Prof. Erick Sperandio is the Lead Researcher and professor of Artificial Intelligence (AI) at SENAI CIMATEC, Bahia, Brazil, also working with Computational Modeling (CM) and HPC. He holds a PhD in Environmental Engineering in the area of Atmospheric Computational Modeling, a Master in Informatics in the field of Computational Intelligence and Graduated in Computer Science from UFES. He currently coordinates, leads and participates in R&D projects in the areas of AI, computational modeling and supercomputing applied to different areas such as Oil and Gas, Health, Advanced Manufacturing, Renewable Energies and Atmospheric Sciences, advising undergraduate, master's and doctoral students. He is the Lead Researcher at SENAI CIMATEC's Reference Center on Artificial Intelligence. In addition, he is a Certified Instructor and University Ambassador of the NVIDIA Deep Learning Institute (DLI) in the areas of Deep Learning, Computer Vision, Natural Language Processing and Recommender Systems, and Principal Investigator of the NVIDIA/CIMATEC AI Joint Lab, the first in Latin America within the NVIDIA AI Technology Center (NVAITC) worldwide program. He also works as a researcher at the Supercomputing Center for Industrial Innovation (CS2i) and at the SENAI Institute of Innovation for Automation (ISI Automação), both from SENAI CIMATEC. He is a member and vice-coordinator of the Basic Board of Scientific-Technological Advice and Evaluation, in the area of Innovation, of the Foundation for Research Support of the State of Bahia (FAPESB). He serves as Technology Transfer Coordinator and one of the Principal Investigators at the National Applied Research Center in Artificial Intelligence (CPA-IA) of SENAI CIMATEC, focusing on Industry, being one of the six CPA-IA in Brazil approved by MCTI / FAPESP / CGI.br. He also participates as one of the representatives of Brazil in the BRICS Innovation Collaboration Working Group on HPC, ICT and AI. He is the coordinator of the Work Group of the Axis 5 - Workforce and Training - of the Brazilian Strategy for Artificial Intelligence (EBIA), and member of the MCTI/EMBRAPII AI Innovation Network Training Committee. He is the coordinator, by SENAI CIMATEC, of the Artificial Intelligence Reference Network of the State of Bahia (REDE BAH.IA). He leads the working group of experts representing Brazil in the Global Partnership on Artificial Intelligence (GPAI), on the theme \"AI and the Pandemic Response\".",institutionString:"Manufacturing and Technology Integrated Campus – SENAI CIMATEC",institution:null},{id:"1063",title:"Prof.",name:"Constantin",middleName:null,surname:"Volosencu",slug:"constantin-volosencu",fullName:"Constantin Volosencu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/1063/images/system/1063.png",biography:"Prof. Dr. Constantin Voloşencu graduated as an engineer from\nPolitehnica University of Timișoara, Romania, where he also\nobtained a doctorate degree. He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:"Polytechnic University of Timişoara",institution:{name:"Polytechnic University of Timişoara",country:{name:"Romania"}}},{id:"221364",title:"Dr.",name:"Eneko",middleName:null,surname:"Osaba",slug:"eneko-osaba",fullName:"Eneko Osaba",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/221364/images/system/221364.jpg",biography:"Dr. Eneko Osaba works at TECNALIA as a senior researcher. He obtained his Ph.D. in Artificial Intelligence in 2015. He has participated in more than twenty-five local and European research projects, and in the publication of more than 130 papers. He has performed several stays at universities in the United Kingdom, Italy, and Malta. Dr. Osaba has served as a program committee member in more than forty international conferences and participated in organizing activities in more than ten international conferences. He is a member of the editorial board of the International Journal of Artificial Intelligence, Data in Brief, and Journal of Advanced Transportation. He is also a guest editor for the Journal of Computational Science, Neurocomputing, Swarm, and Evolutionary Computation and IEEE ITS Magazine.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"275829",title:"Dr.",name:"Esther",middleName:null,surname:"Villar-Rodriguez",slug:"esther-villar-rodriguez",fullName:"Esther Villar-Rodriguez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/275829/images/system/275829.jpg",biography:"Dr. Esther Villar obtained a Ph.D. in Information and Communication Technologies from the University of Alcalá, Spain, in 2015. She obtained a degree in Computer Science from the University of Deusto, Spain, in 2010, and an MSc in Computer Languages and Systems from the National University of Distance Education, Spain, in 2012. Her areas of interest and knowledge include natural language processing (NLP), detection of impersonation in social networks, semantic web, and machine learning. Dr. Esther Villar made several contributions at conferences and publishing in various journals in those fields. Currently, she is working within the OPTIMA (Optimization Modeling & Analytics) business of TECNALIA’s ICT Division as a data scientist in projects related to the prediction and optimization of management and industrial processes (resource planning, energy efficiency, etc).",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"49813",title:"Dr.",name:"Javier",middleName:null,surname:"Del Ser",slug:"javier-del-ser",fullName:"Javier Del Ser",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49813/images/system/49813.png",biography:"Prof. Dr. Javier Del Ser received his first PhD in Telecommunication Engineering (Cum Laude) from the University of Navarra, Spain, in 2006, and a second PhD in Computational Intelligence (Summa Cum Laude) from the University of Alcala, Spain, in 2013. He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. He is a Senior Member of the IEEE, and a recipient of the Biscay Talent prize for his academic career.",institutionString:"Tecnalia Research & Innovation",institution:null},{id:"278948",title:"Dr.",name:"Carlos Pedro",middleName:null,surname:"Gonçalves",slug:"carlos-pedro-goncalves",fullName:"Carlos Pedro Gonçalves",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRcmyQAC/Profile_Picture_1564224512145",biography:'Carlos Pedro Gonçalves (PhD) is an Associate Professor at Lusophone University of Humanities and Technologies and a researcher on Complexity Sciences, Quantum Technologies, Artificial Intelligence, Strategic Studies, Studies in Intelligence and Security, FinTech and Financial Risk Modeling. He is also a progammer with programming experience in:\n\nA) Quantum Computing using Qiskit Python module and IBM Quantum Experience Platform, with software developed on the simulation of Quantum Artificial Neural Networks and Quantum Cybersecurity;\n\nB) Artificial Intelligence and Machine learning programming in Python;\n\nC) Artificial Intelligence, Multiagent Systems Modeling and System Dynamics Modeling in Netlogo, with models developed in the areas of Chaos Theory, Econophysics, Artificial Intelligence, Classical and Quantum Complex Systems Science, with the Econophysics models having been cited worldwide and incorporated in PhD programs by different Universities.\n\nReceived an Arctic Code Vault Contributor status by GitHub, due to having developed open source software preserved in the \\"Arctic Code Vault\\" for future generations (https://archiveprogram.github.com/arctic-vault/), with the Strategy Analyzer A.I. module for decision making support (based on his PhD thesis, used in his Classes on Decision Making and in Strategic Intelligence Consulting Activities) and QNeural Python Quantum Neural Network simulator also preserved in the \\"Arctic Code Vault\\", for access to these software modules see: https://github.com/cpgoncalves. He is also a peer reviewer with outsanding review status from Elsevier journals, including Physica A, Neurocomputing and Engineering Applications of Artificial Intelligence. Science CV available at: https://www.cienciavitae.pt//pt/8E1C-A8B3-78C5 and ORCID: https://orcid.org/0000-0002-0298-3974',institutionString:"University of Lisbon",institution:{name:"Universidade Lusófona",country:{name:"Portugal"}}},{id:"241400",title:"Prof.",name:"Mohammed",middleName:null,surname:"Bsiss",slug:"mohammed-bsiss",fullName:"Mohammed Bsiss",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/241400/images/8062_n.jpg",biography:null,institutionString:null,institution:null},{id:"276128",title:"Dr.",name:"Hira",middleName:null,surname:"Fatima",slug:"hira-fatima",fullName:"Hira Fatima",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/276128/images/14420_n.jpg",biography:"Dr. Hira Fatima\nAssistant Professor\nDepartment of Mathematics\nInstitute of Applied Science\nMangalayatan University, Aligarh\nMobile: no : 8532041179\nhirafatima2014@gmal.com\n\nDr. Hira Fatima has received his Ph.D. degree in pure Mathematics from Aligarh Muslim University, Aligarh India. Currently working as an Assistant Professor in the Department of Mathematics, Institute of Applied Science, Mangalayatan University, Aligarh. She taught so many courses of Mathematics of UG and PG level. Her research Area of Expertise is Functional Analysis & Sequence Spaces. She has been working on Ideal Convergence of double sequence. She has published 17 research papers in National and International Journals including Cogent Mathematics, Filomat, Journal of Intelligent and Fuzzy Systems, Advances in Difference Equations, Journal of Mathematical Analysis, Journal of Mathematical & Computer Science etc. She has also reviewed few research papers for the and international journals. She is a member of Indian Mathematical Society.",institutionString:null,institution:null},{id:"414880",title:"Dr.",name:"Maryam",middleName:null,surname:"Vatankhah",slug:"maryam-vatankhah",fullName:"Maryam Vatankhah",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Borough of Manhattan Community College",country:{name:"United States of America"}}},{id:"414879",title:"Prof.",name:"Mohammad-Reza",middleName:null,surname:"Akbarzadeh-Totonchi",slug:"mohammad-reza-akbarzadeh-totonchi",fullName:"Mohammad-Reza Akbarzadeh-Totonchi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Ferdowsi University of Mashhad",country:{name:"Iran"}}},{id:"414878",title:"Prof.",name:"Reza",middleName:null,surname:"Fazel-Rezai",slug:"reza-fazel-rezai",fullName:"Reza Fazel-Rezai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"American Public University System",country:{name:"United States of America"}}},{id:"302698",title:"Dr.",name:"Yao",middleName:null,surname:"Shan",slug:"yao-shan",fullName:"Yao Shan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Dalian University of Technology",country:{name:"China"}}},{id:"125911",title:"Prof.",name:"Jia-Ching",middleName:null,surname:"Wang",slug:"jia-ching-wang",fullName:"Jia-Ching Wang",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"National Central University",country:{name:"Taiwan"}}},{id:"357085",title:"Mr.",name:"P. Mohan",middleName:null,surname:"Anand",slug:"p.-mohan-anand",fullName:"P. Mohan Anand",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"356696",title:"Ph.D. Student",name:"P.V.",middleName:null,surname:"Sai Charan",slug:"p.v.-sai-charan",fullName:"P.V. Sai Charan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"357086",title:"Prof.",name:"Sandeep K.",middleName:null,surname:"Shukla",slug:"sandeep-k.-shukla",fullName:"Sandeep K. Shukla",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"356823",title:"MSc.",name:"Seonghee",middleName:null,surname:"Min",slug:"seonghee-min",fullName:"Seonghee Min",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Daegu University",country:{name:"Korea, South"}}},{id:"353307",title:"Prof.",name:"Yoosoo",middleName:null,surname:"Oh",slug:"yoosoo-oh",fullName:"Yoosoo Oh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:"Yoosoo Oh received his Bachelor's degree in the Department of Electronics and Engineering from Kyungpook National University in 2002. He obtained his Master’s degree in the Department of Information and Communications from Gwangju Institute of Science and Technology (GIST) in 2003. In 2010, he received his Ph.D. degree in the School of Information and Mechatronics from GIST. In the meantime, he was an executed team leader at Culture Technology Institute, GIST, 2010-2012. In 2011, he worked at Lancaster University, the UK as a visiting scholar. In September 2012, he joined Daegu University, where he is currently an associate professor in the School of ICT Conver, Daegu University. Also, he served as the Board of Directors of KSIIS since 2019, and HCI Korea since 2016. From 2017~2019, he worked as a center director of the Mixed Reality Convergence Research Center at Daegu University. From 2015-2017, He worked as a director in the Enterprise Supporting Office of LINC Project Group, Daegu University. His research interests include Activity Fusion & Reasoning, Machine Learning, Context-aware Middleware, Human-Computer Interaction, etc.",institutionString:null,institution:{name:"Daegu Gyeongbuk Institute of Science and Technology",country:{name:"Korea, South"}}},{id:"262719",title:"Dr.",name:"Esma",middleName:null,surname:"Ergüner Özkoç",slug:"esma-erguner-ozkoc",fullName:"Esma Ergüner Özkoç",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Başkent University",country:{name:"Turkey"}}},{id:"346530",title:"Dr.",name:"Ibrahim",middleName:null,surname:"Kaya",slug:"ibrahim-kaya",fullName:"Ibrahim Kaya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"419199",title:"Dr.",name:"Qun",middleName:null,surname:"Yang",slug:"qun-yang",fullName:"Qun Yang",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Auckland",country:{name:"New Zealand"}}}]}},subseries:{item:{id:"1",type:"subseries",title:"Oral Health",keywords:"Oral health, Dental care, Diagnosis, Diagnostic imaging, Early diagnosis, Oral cancer, Conservative treatment, Epidemiology, Comprehensive dental care, Complementary therapies, Holistic health",scope:"\r\n This topic aims to provide a comprehensive overview of the latest trends in Oral Health based on recent scientific evidence. Subjects will include an overview of oral diseases and infections, systemic diseases affecting the oral cavity, prevention, diagnosis, treatment, epidemiology, as well as current clinical recommendations for the management of oral, dental, and periodontal diseases.
",coverUrl:"https://cdn.intechopen.com/series_topics/covers/1.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11397,editor:{id:"173955",title:"Prof.",name:"Sandra",middleName:null,surname:"Marinho",slug:"sandra-marinho",fullName:"Sandra Marinho",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRGYMQA4/Profile_Picture_2022-06-01T13:22:41.png",biography:"Dr. Sandra A. Marinho is an Associate Professor and Brazilian researcher at the State University of Paraíba (Universidade Estadual da Paraíba- UEPB), Campus VIII, located in Araruna, state of Paraíba since 2011. She holds a degree in Dentistry from the Federal University of Alfenas (UNIFAL), while her specialization and professional improvement in Stomatology took place at Hospital Heliopolis (São Paulo, SP). Her qualifications are: a specialist in Dental Imaging and Radiology, Master in Dentistry (Periodontics) from the University of São Paulo (FORP-USP, Ribeirão Preto, SP), and Doctor (Ph.D.) in Dentistry (Stomatology Clinic) from Hospital São Lucas of the Pontifical Catholic University of Rio Grande do Sul (HSL-PUCRS, Porto Alegre, RS). She held a postdoctoral internship at the Federal University from Jequitinhonha and Mucuri Valleys (UFVJM, Diamantina, MG). She is currently a member of the Brazilian Society for Dental Research (SBPqO) and the Brazilian Society of Stomatology and Pathology (SOBEP). Dr. Marinho's experience in Dentistry mainly covers the following subjects: oral diagnosis, oral radiology; oral medicine; lesions and oral infections; oral pathology, laser therapy and epidemiological studies.",institutionString:null,institution:{name:"State University of Paraíba",institutionURL:null,country:{name:"Brazil"}}},editorTwo:null,editorThree:null,series:{id:"3",title:"Dentistry",doi:"10.5772/intechopen.71199",issn:"2631-6218"},editorialBoard:[{id:"267724",title:"Dr.",name:"Febronia",middleName:null,surname:"Kahabuka",slug:"febronia-kahabuka",fullName:"Febronia Kahabuka",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRZpJQAW/Profile_Picture_2022-06-27T12:00:42.JPG",institutionString:null,institution:null}]},onlineFirstChapters:{paginationCount:6,paginationItems:[{id:"82135",title:"Carotenoids in Cassava (Manihot esculenta Crantz)",doi:"10.5772/intechopen.105210",signatures:"Lovina I. Udoh, Josephine U. Agogbua, Eberechi R. 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