Transmission electron microscopy (TEM) gives a good image for ultra-intracellular organelles in two-dimensional projections, but to get a three-dimensional structural information, we should use the high-voltage transmission electron tomography (TET). The use of TET is important to resolve the questions about the relation between the different cell organelles and their mechanisms of action to correlate structure to function for medical treatment solutions.
Part of the book: Electron Microscopy