Biolistic is a special high-performance method for direct delivery of foreign DNA, RNA, or protein into plant cells. This method has less physiological risk on plant cell since there is no need for microbial intermediaries (Agrobacterium strains) and requires less additional DNA. Moreover, it can adapt for both monocotyledon and dicotyledonous plants. Recently, this method has also been successfully used to plant genome editing. Therefore, in this chapter, we discuss the application of this method for genetic improvement of some commercially important of tropical and subtropical fruit trees including banana, date palm, citrus, mango, olive, and pineapple. Also, we explain the details of biolistic protocols used for transient and stable gene expression in these fruit trees.
Part of the book: Transgenic Crops