Variations in MSW composition in Bangalore City from 1999 to 2013.
\\n\\n
Released this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\\n\\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\\n"}]',published:!0,mainMedia:{caption:"Highly Cited",originalUrl:"/media/original/117"}},components:[{type:"htmlEditorComponent",content:'IntechOpen is proud to announce that 191 of our authors have made the Clarivate™ Highly Cited Researchers List for 2020, ranking them among the top 1% most-cited.
\n\nThroughout the years, the list has named a total of 261 IntechOpen authors as Highly Cited. Of those researchers, 69 have been featured on the list multiple times.
\n\n\n\nReleased this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\n\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
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Koopman-van Gemert",profilePictureURL:"https://mts.intechopen.com/storage/users/105746/images/5803_n.jpg",biography:"Dr. Anna Wilhelmina Margaretha Maria Koopman-van Gemert MD, PhD, became anaesthesiologist-intensivist from the Radboud University Nijmegen (the Netherlands) in 1987. She worked for a couple of years also as a blood bank director in Nijmegen and introduced in the Netherlands the Cell Saver and blood transfusion alternatives. She performed research in perioperative autotransfusion and obtained the degree of PhD in 1993 publishing Peri-operative autotransfusion by means of a blood cell separator.\nBlood transfusion had her special interest being the president of the Haemovigilance Chamber TRIP and performing several tasks in local and national blood bank and anticoagulant-blood transfusion guidelines committees. Currently, she is working as an associate professor and up till recently was the dean at the Albert Schweitzer Hospital Dordrecht. She performed (inter)national tasks as vice-president of the Concilium Anaesthesia and related committees. \nShe performed research in several fields, with over 100 publications in (inter)national journals and numerous papers on scientific conferences. \nShe received several awards and is a member of Honour of the Dutch Society of Anaesthesia.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Albert Schweitzer Hospital",institutionURL:null,country:{name:"Gabon"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"1030",title:"Immunohaematology",slug:"immunohaematology"}],chapters:[{id:"55513",title:"A Double In Vivo Biotinylation Technique to Assess Erythrocyte Turnover in Blood Circulation",slug:"a-double-in-vivo-biotinylation-technique-to-assess-erythrocyte-turnover-in-blood-circulation",totalDownloads:1575,totalCrossrefCites:0,authors:[{id:"202626",title:"Prof.",name:"Rajiv",surname:"Saxena",slug:"rajiv-saxena",fullName:"Rajiv Saxena"}]},{id:"55207",title:"Immunocamouflaged RBC for Alloimmunized Patients",slug:"immunocamouflaged-rbc-for-alloimmunized-patients",totalDownloads:1545,totalCrossrefCites:1,authors:[{id:"202243",title:"Dr.",name:"Mark",surname:"Scott",slug:"mark-scott",fullName:"Mark Scott"},{id:"205640",title:"BSc.",name:"Wendy",surname:"Toyofuku",slug:"wendy-toyofuku",fullName:"Wendy Toyofuku"},{id:"205641",title:"BSc.",name:"Xining",surname:"Yang",slug:"xining-yang",fullName:"Xining Yang"},{id:"205642",title:"Dr.",name:"Meera",surname:"Raj",slug:"meera-raj",fullName:"Meera Raj"},{id:"205643",title:"Dr.",name:"Ning",surname:"Kang",slug:"ning-kang",fullName:"Ning Kang"}]},{id:"55954",title:"Red Blood Cells and Relation to Thrombosis",slug:"red-blood-cells-and-relation-to-thrombosis",totalDownloads:2067,totalCrossrefCites:4,authors:[{id:"202814",title:"Associate Prof.",name:"Anil",surname:"Tombak",slug:"anil-tombak",fullName:"Anil Tombak"}]},{id:"55635",title:"Platelet and Immunity in Transfusion Medicine",slug:"platelet-and-immunity-in-transfusion-medicine",totalDownloads:1464,totalCrossrefCites:1,authors:[{id:"200979",title:"Prof.",name:"Xingbin",surname:"Hu",slug:"xingbin-hu",fullName:"Xingbin Hu"},{id:"206182",title:"Ms.",name:"Jinmei",surname:"Xu",slug:"jinmei-xu",fullName:"Jinmei Xu"}]},{id:"55343",title:"Red Blood Cell Transfusion and Functional Dose",slug:"red-blood-cell-transfusion-and-functional-dose",totalDownloads:1289,totalCrossrefCites:0,authors:[{id:"202960",title:"Prof.",name:"Deqing",surname:"Wang",slug:"deqing-wang",fullName:"Deqing Wang"},{id:"202995",title:"Dr.",name:"Leiying",surname:"Zhang",slug:"leiying-zhang",fullName:"Leiying Zhang"}]},{id:"55676",title:"Transfusion in Transplantation",slug:"transfusion-in-transplantation",totalDownloads:1878,totalCrossrefCites:0,authors:[{id:"94230",title:"Prof.",name:"Guray",surname:"Saydam",slug:"guray-saydam",fullName:"Guray Saydam"},{id:"94231",title:"Prof.",name:"Fahri",surname:"Sahin",slug:"fahri-sahin",fullName:"Fahri Sahin"},{id:"202813",title:"M.D.",name:"Eren",surname:"Arslan Davulcu",slug:"eren-arslan-davulcu",fullName:"Eren Arslan Davulcu"}]},{id:"55256",title:"Red Blood Cell Transfusion Strategy for Upper Gastrointestinal Bleeding",slug:"red-blood-cell-transfusion-strategy-for-upper-gastrointestinal-bleeding",totalDownloads:1413,totalCrossrefCites:0,authors:[{id:"197501",title:"Dr.",name:"Xingshun",surname:"Qi",slug:"xingshun-qi",fullName:"Xingshun Qi"},{id:"205228",title:"Prof.",name:"Fernando",surname:"Romeiro",slug:"fernando-romeiro",fullName:"Fernando Romeiro"},{id:"207599",title:"Prof.",name:"Yiling",surname:"Li",slug:"yiling-li",fullName:"Yiling Li"}]}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"177731",firstName:"Dajana",lastName:"Pemac",middleName:null,title:"Ms.",imageUrl:"https://mts.intechopen.com/storage/users/177731/images/4726_n.jpg",email:"dajana@intechopen.com",biography:"As a Commissioning Editor at IntechOpen, I work closely with our collaborators in the selection of book topics for the yearly publishing plan and in preparing new book catalogues for each season. This requires extensive analysis of developing trends in scientific research in order to offer our readers relevant content. Creating the book catalogue is also based on keeping track of the most read, downloaded and highly cited chapters and books and relaunching similar topics. I am also responsible for consulting with our Scientific Advisors on which book topics to add to our catalogue and sending possible book proposal topics to them for evaluation. Once the catalogue is complete, I contact leading researchers in their respective fields and ask them to become possible Academic Editors for each book project. Once an editor is appointed, I prepare all necessary information required for them to begin their work, as well as guide them through the editorship process. I also assist editors in inviting suitable authors to contribute to a specific book project and each year, I identify and invite exceptional editors to join IntechOpen as Scientific Advisors. 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As civilization advanced, humans directly or indirectly interfered with the natural environment. This led to an imbalance in the human-nature relationship, finally leading to environmental problems like soil, air, and water pollution and accumulation of municipal solid waste (MSW).
In olden days, MSW disposal did not pose significant problems because the population was very less and the availability of land for the dumping of wastes was large. But these days MSWM is a serious problem everywhere. Due to rapid industrialization and increased population levels, the generation rate of MSW in metropolitan cities accelerates. This has led to the migration of people from villages to cities, which generates thousands of tons of MSW daily with rapid change in the quantity and character of the waste in line with the changing lifestyle of the people and also with the changes in the market technology, building technology, and fuel technology.
The environmental degradation and energy crisis are two significant issues for global sustainable development. Due to rapid urbanization, industrialization and increase of the growth of population have led to severe substantial waste management problems in several developing countries like India, Malaysia, Nepal, and Bangladesh. As the village develops into towns and cities, in developing countries the disposal of solid waste onto access ways, empty lands, and waterways has been witnessed. Presently, more materials are consumed than required to meet their daily needs by a greedy human. Human beings generate domestic, agricultural, industrial, and medical wastes at every level of development. This waste comprises of both solid and semisolid organic wastes, which may be biodegradable and non-biodegradable. Hence, proper collection and subsequent disposal of waste assumed vital importance in community environmental sanitation programs.
MSW has become one of the significant environmental issues, particularly in developing countries. The solid waste generation mainly consists of biodegradable and non-biodegradable waste materials produced due to several societal activities. The improper dumping of solid waste pollutes the air, soil, and water. The BBMP with an area of 2190 km2 and a population of about 10.18 million generates around 5000 metric tons per day of waste at an average generation rate of 0.5 kg per capita per day (kg/capita/d). Presently, Bengaluru is facing significant problems due to existing disposal practices of generated waste incurring high cost due to lack of proper infrastructural facilities; also the open dumping in the expanding zone of the city poses severe problems to the structures constructed on these old dumps. In the meantime, groundwater quality deteriorated due to improper leachate management [1].
In Bengaluru, there are more than 60 illegal dumpsites identified. While BBMP and the Karnataka State Pollution Control Board (KSPCB) close these dumpsites, the new ones emerge elsewhere, posing health risks to residents in their vicinity. The MSW (Management and Handling) Rules, 2000, recommend source-specific waste collection and transportation in addition to appropriate processing and disposal. There is a lack of knowledge of the quantity and characteristics of reliable waste aids in the preparation of a long-term plan for an MSWM system. So, it was deemed necessary by the BBMP to assess the current status of the municipal solid waste management system in Bengaluru [2].
In this context, the present study discusses the potential improvement in handling the wastes and reduces the amount of waste to be transported and dumped in the landfill. If this waste is used for energy and nutrient recovery, decentralization could also become commercially viable. Moreover, it also addresses the wise technology deficiencies in the existing MSWM system of Bengaluru.
The city of Bengaluru (12.98°N and 77.58°E) in Karnataka is the state capital, and it has a mild and salubrious climate. It is located at an elevation of 900 m. Since the 1980s, Bengaluru has enjoyed the reputation of being one of the fastest developing cities in Asia [3]. The Bengaluru Metropolitan Area covers an area of 1258 sq. km and is the fifth largest city in India. However, with an increased population level, rapid economic growth, and a rise in community living standard, the generation rate of MSW in metropolitan cities accelerates. The local authorities are struggling to provide the proper solid waste management system to a satisfactory level. Recently the authorities have taken initiatives and measures to organize the MSWM sector. This research would help to identify techniques suitable for the current scenario, the loopholes in the adopted methods, and the possible alternatives.
The BBMP has a city council that consists of 123 elected members or councilors, each representing a ward. Both the mayor and deputy mayor are chosen from among councilors for a 1-year term. The BBMP has 15,000 employees and is headed by the commissioner. The commissioner is the head of the BBMP, appointed and deputed by the State Government of Karnataka and responsible for performing duties and functions.
Presently, the Bruhat Bengaluru Mahanagara Palike is the agency vested with responsibility for effective solid waste management system for the Bengaluru City. For a more efficient and effective approach, the Bengaluru City has been divided into different administrative units. There are 294 health wards within the BBMP. Presently, in Bengaluru, there are 198 such administrative or political wards (Figure 1). Within the BBMP, there are two departments, namely, the health department and engineering department. The health department is mainly responsible for the collection, transportation, and disposal of solid waste. The engineering department handles the removal of construction and demolition waste, while they also provide technical and infrastructural support to the health department.
BBMP Zonal organization chart.
At present, 10% of solid waste is recycled in Bengaluru. Most of the literature reported that the waste generation rate is 0.4–0.6 kg/capita/day. The proposed waste generation rate is about 0.5 kg/capita/day in Bengaluru [4]. Since 1990, the composition of India’s urban wastes has changed drastically. At present, the waste generation is about 5000 metric tons, and waste generation is likely to grow over the coming years. Going by the present trend of increase in the quantity of waste, the waste quantity projected for the next 20 years is shown in Figure 2.
Waste quantity expected for the next 20 years.
The changes in the composition of MSW should form essential criteria for any waste management system. Hence, the data available on the composition of the waste from different sources over the years have been collected and analyzed. Figure 3 shows variation in MSW composition from 1999 to 2013 in Bengaluru City. With the increase in urbanization and change in food habits and lifestyle, the amount of MSW has been multiplying, and there is variation in waste composition.
Bangalore’s urban waste composition.
The changes in the composition of MSW should form essential criteria for any waste management system. Hence, the data available on the composition of the waste from different sources over the years has been collected and analyzed. The data presented in Table 1 was statistically analyzed to get the variations of a different type of waste in rapidly growing cities like Bangalore.
Year | Biodegradable | Paper/leather/cardboard | Rubber/debris | Glass | Plastic | Metals | Others |
---|---|---|---|---|---|---|---|
1999 | 42 | 16 | 10 | 3 | 7 | 2 | 20 |
2000 | 45 | 13 | 9 | 6 | 6 | 3 | 18 |
2007 | 59 | 12 | 5 | 3 | 12 | 1 | 8 |
2013 | 61 | 9 | 4 | 1 | 7 | 1 | 16 |
The MSW composition generated in Bengaluru has changed considerably from 1999 to 2013, which is evident in Table 1. It was observed that there was an increase in the biodegradable percentage in Bengaluru City from 42% in 1999 to 61% in 2013, indicating increased organic waste generation in the city, which may be primarily due to increasing population, improper solid waste management, or accumulation of green waste. Similarly, there was a 16% decrease in the paper, cardboard, and leather wastes in Bengaluru City, indicating recycling activities of paper and cardboard. A considerable increase in plastic wastes was also observed in 2007, which might be due to the urbanization and increased use of plastic carry bags. In 2013, the percentage of plastic waste decreased to 7%, which may be attributable to the effective ban of plastics carry bags below 40microns within city limits in 2012. The glass, metals, and rubber fraction observed a decrease in MSW composition, indicating the decreased use of glass and metal products and effective recycling of glass and metal products by segregation at sources itself.
The variations in MSW composition shown in Figure 4 can be utilized in choosing the best method of MSW disposal in Bengaluru City. Biodegradable percentage (61% in 2013), more than 50%, suggests employment of methods, such as windrow composting, community composting process, pyrolysis, and vermicomposting, which assists in manure generation for agricultural practices; also, biomethanation can be employed to produce biogas, which can be utilized as a fuel or alternative source for electricity generation. As the significant composition of MSW is organic, waste treatment options like composting were successful in Bangalore, and 3.14% waste reduction was achieved through composting [8].
Variations in MSW composition from 1999 to 2013 in Bangalore City.
The objective of solid waste management is to remove discarded materials from inhabited places promptly to prevent the spread of disease, to reduce esthetic results arising from purifying organic matter, and, equally important, to dispose of the discarded materials in a manner that is environmentally acceptable [9].
Currently, in Bengaluru, source segregation is still a concern through awareness in picking up slowly. BBMP handles about 30% of solid waste, and the remaining waste activity is outsourced (starting from primary collection to disposal). Solid waste collection is carried out in two phases. The first phase is a primary collection, in which the solid waste is collected on auto tipper and pushcarts. An auto tipper has been provided for every 1000 households and a cart for every 200 homes. About 20,000 pourakarmikas are being utilized (both BBMP and contractors) in the door to door collection, street sweeping, and transportation of MSW. The collected solid waste from houses is brought to a common point, i.e., secondary locations from where the waste is transferred to landfill sites/treatment through tipper lorries and compactors. Figure 5 indicates a typical scheme of how the collection and transportation are being practiced in most of the wards.
MSW collection system.
This activity is assigned to self-help groups (SHGs), which are basically below poverty women’s groups. BBMP has allocated 3197 pourakarmikas (sweepers) and 18,562 pourakarmikas from a contractor who performs door to door collection and sweeping activities. Annually about 250 crores are spent on solid waste management, i.e., BBMP pourakarmika salary, contract payment, and tipping fees (Figure 6).
MSW collection process in a typical residential/commercial area.
The survey carried out in Malleswaram by [10] indicated that no norms/guidelines had been followed in setting up waste segregation practices adopted in this ward. There is a lack of awareness among people that leads to confusion. The six categories for segregation mandated by BBMP are overwhelming and are a deterrent to segregation. The waste collectors (pourakarmikas) lack training in proper segregation practices and its importance. Incomplete segregation is the predominant practice currently, and steps to realize a higher level of compliance and efficiency need to be effected.
Transportation of waste from collection centers to the landfill site is another crucial step in waste management. At present, waste transportation is using pushcarts, auto, etc., which bring waste to primary collection centers. From there, trucks collect the municipal solid waste and transport it to waste disposal sites/landfill.
The issues in transporting waste are mentioned below:
The waste spills from the trucks due to open beds in trucks and tractors, during transport, thereby causing a nuisance.
Solid waste loaded manually in a truck without using the protective gears is dangerous to the health of workers.
A secondary storage system is not well synchronized with the transport system. Problems arise when a transport fleet is modernized because waste at a secondary storage system is still dumped on the ground.
The area cannot be appropriately serviced due to an inadequate number of vehicles.
Due to inadequate workshop facilities and maintenance procedures, the vehicles are poorly maintained. This problem leads to a breakdown of trucks, and they become out of service for a long time.
Based on the above-presented data and analysis, for effective solid waste management, the following suggestion can be made:
Establish the segregation at the source itself, and encourage by giving incentive to the contractors with the performance in segregation.
Establish the wet waste processing units for composting, and encourage decentralized processing for dry waste collection center at the source.
Set up the segregation units and zone-wise processing facilities to ensure 100% processing of municipal solid waste, and minimize the solid waste quantity going to the landfill sites.
Reduce transportation of municipal solid waste using the above measures of decentralized as well as zone-wise processing units.
Currently Bengaluru does not have any appropriate scientific treatment techniques for waste generated by municipal and industries around Bengaluru. This has led to the development of various unauthorized dumpsites. The solid waste, generated from hotels, restaurants, Kalyana mandapas, markets, etc., is being directly collected and transported to the treatment/disposal facilities. The treatment facilities have been developed around the city, and their spread over the zone is set out in Table 2.
Sl. No | Zone | Existing disposal site/facility |
---|---|---|
1 | South | Bingipura, Mavallipura, KCDC |
2 | East | MSGP, Mavallipura, KCDC |
3 | West | Terra firma, Mavallipura, KCDC |
4 | Yelahanka | Mavallipura, Terrafirma |
5 | Bommanahalli | Bingipura, Laxmipura |
6 | Mahadevapura | Terrafirma |
7 | Dasarahalli | MSGP |
8 | Raja Rajeshwari Nagar | MSGP/Terrafirma |
Existing treatment and disposal facilities in BBMP.
For making effective solid waste management services, it is essential to select appropriate technology, which can suit and work in the given area successfully. Simultaneously, proper measures have to be considered for institutional strengthening and internal capacity building. Institutional strengthening can be done by adequately decentralizing the administration, delegating adequate powers at the decentralized level, providing training to the existing staff, and assigning the responsibility for the workforce as well as for supervisory staff. NGO/private sector participation is necessary for making service competitive and efficient. The land is scarce, and public health and environmental resources are precious.
In this direction, many decentralized facilities are being established. The decentralization of administration has to be implemented in large cities to make solid waste management service effectively. Decentralization can be divided into three tiers—one at the ward level, second at the zone level, and third at the city level. The BBMP established the decentralized processing units for dry waste; details are as follows.
Dry waste materials like batteries, tin cans, plastic-coated milk cartons, nylon, cigarette butts, and leather all take varying lengths of time to degrade: not less than 10 years. Hence, the best way to dispose of these dry waste items is to reuse/recycle them. Nearly 70% of all the dry waste thrown away can be safely disposed of this way.
The aim of Dry Waste Collection Centers (DWCCs) is to keep as much waste out of the landfills/waste dumps as possible and to help make waste useful and profitable. DWCCs run by various agencies in Bengaluru in coordination with the BBMP is decentralized bulk sorting and processing facilities. About 185 DWCCs have been established and functional. DWCCs are set up on municipal/government/private lands and various NGOs, waste pickers, and contractors; self-help groups have been involved for effective functioning. The dry waste generated in the wards is collected and further segregated and sent for recycling from these recycling centers. Receiving in bulk provides these informal sector workers with more significant returns and creates more jobs.
Currently, Bengaluru can handle the waste of about 2100 TPD. The existing capacity of waste treatment facility at Mavallipura is 600TPD, Karnataka Composting Development Corporation Ltd. (KCDC) is 300TPD, and Terra Firma is about 1500 TPD as shown in Figure 7. For achieving 100% processing of solid waste, the government has identified new landfill sites. These sites are being set up at the following locations: Kannahalli (500TPD), Seegihalli (200TPD), Doddabidarakallu (200TPD), Lingaderenahalli (200TPD), Subrayanpalya (200TPD), Chikkanagamangala (500TPD), and KCDC (upgradation) (500TPD). Majorly we waste composting plants with a provision to screen compost out of mixed MSW and provision also to store the non-compostable/non-recyclable materials. These materials can be used for co-incineration in cement industries/power generation [4].
Sanitary landfill sites in Bangalore.
Once the solid waste is collected from the different sectors of the community, the next problem is regarding the safe, economical, and efficient disposal options. Suitable decisions have to be made in this regard to avoid illegal dumping and open dumping of solid wastes that are dangerous and a threat to the environment. Open burning of solid wastes releases smoke containing pollutants harmful to human health and the environment. Therefore, the community has to face severe inconveniences due to illegal dumping practices. Hence, conventional methods employed to safe disposal options of MSW include composting, waste to energy (such as biogas production and incineration), and landfilling. Incineration and composting of MSW are a standard solid waste treatment or processing methods, as they produce secondary waste such as non-biodegradable material rejects from composting and ash from incineration that needs to be disposed of further [11].
Aerobic composting process involves piling up of waste and requires regular turning, manually or by mechanical devices, and sufficient air, and oxygen has to be provided during the decomposition by bacteria, fungi, and microorganisms like actinomycetes. A mesophilic bacterium is an initial process, which oxidizes the organic matter to carbon dioxide and generates the heat and temperature rise to about 45°C. In the next process, thermophilic bacteria continue the decomposition; in this phase, temperature further rises to about 60°C. Three weeks is required for stabilized compost and fall in temperature of the compost mass. The final product of the compost should have a dark brown color and earthy smell.
The waste is dumped in the windrow platform; large items like woods, plastics, clothes, thermocol, etc. are removed; and inoculum will be sprayed on the waste. The inoculum will be prepared using the mixture of bacteria, cow dung, and water. The treated waste is then heaped in windrows with long rows approximately 2 meters in height and 3 meters in width; length will be depending on the size of the landfill site. There are seven rows, each row for each day of the week. Every week these rows are turned for 5 weeks. These rows are turned to remove moisture, improve porosity and oxygen content, and redistribute hotter and colder portions of the pile. As time passes, the sizes of the rows get reduced due to the decomposition of the waste and the resultant volume reduction. Hence, the number of final rows will be decreased than the number of initial rows. Composting will be completed in 25–30 days. This interval is known as maturation in which waste will undergo mechanical process operation. In mechanical processing, sieving occurs in three stages: in the first stage, sieve employs 36 mm mesh, the second stage applies 16 mm mesh, and the third stage has a 4 mm mesh, as shown in Figure 8. At each stage of sieving the reject, materials are separated and either reused or disposed of at the landfill. Any leachate or runoff created must be collected and treated. To avoid problems with leachate or runoff, waste piles can be placed under a roof, but doing so adds to the initial costs of the operation [11].
Windrow composting process.
The following are the challenges in windrow composting:
Minor mechanical fault leads to a breakdown due to the unavailability of spare parts.
The major difficulty is due to the nature of waste. Pulverizers get frequently clogged with pieces of plastic, rubber, leathers, etc., and due to metal and glass pieces, the blades breakdown. If waste is mixed with soil, it causes a problem in the process, lowering the quality produced.
Lack of continuous power supply.
In the rainy season, the process cannot be carried out.
In this process, daily wet waste is collected by the housekeeping staff, directly dumping into the tank. After filling, the tank is covered with a layer of refuse 15–20 cm deep. The materials are allowed to remain in the pit without turning and watering for 3 months. To keep the decomposers working, the aeration aid is needed during the initial pile construction. As long as plenty of air is available, aerobic decomposers work faster and more efficiently, providing you with finished compost on a more rapid time. Charcoal is placed in the tank. Hence, foul smell is avoided. It takes about 3 months to obtain the finished product. Community composting process is shown in Figure 9.
Community composting process.
In this process, organic waste such as vegetable and fruit peels and food leftovers, bones, meat, eggshells, household sweeping dry leaves, garden waste, cattle dung, etc. collected from the apartments and other places were identified for segregation for removal of plastic, glass, clothes, paper, leather, etc. for recycling purposes. After segregation of organic waste, it is then fed into the mechanical unit (i.e., organic waste converter) which converts this into a homogenized, crushed, odor-free output (Figure 10). The output goes to the curing system for stabilization. Aerobic microbial decomposition controls the entire process; the transition takes place from low pH levels to high pH levels and then stabilizes. This manure is free from weed, foul smell, and pathogen as the process is aerobic. This is environment-friendly operation; this system takes only 15 min to convert the organic waste into a homogenized output.
Biomechanical process.
More than 50,000 populations of worms can support the moist compost heap of 2.4 m by 1.2 m and 0.6 m high. Organic residues such as straw and other crop residues, animal manure, green weeds, and leaves are filled in the pit and covered loosely with soil and kept moist for a weak. On the top of the heap, well-watered, the worms will be introduced, and air is provided for quick decomposition.
Vermicomposting process.
The following are challenges in vermicomposting:
This concept is suitable for only small-scale applications and not an appropriate solution for large-scale application, e.g., 100–300MT/d capacity plants.
The exotic species are found to be costing between Rs. 500 and 1000/kg, and indigenous species of earthworms are not found useful.
The raw waste cannot be fed directly to earthworms, thus necessitating the pre-processing of waste to avoid toxicity.
Earthworms are so sensitive to temperature (ideally between 20 and 28°C); worms die due to heat built up in the rotting pile or summer.
Some demonstrated technology approaches are available for waste-to-energy (WTE) projects today which are anaerobic processing/biogas production, refuse-derived fuel (RDF), and plasma gasification.
Biomethaization plants are being established for wet solid waste at 16 locations, out of which 8 have been made functional. 400 units are generated per day per plant. The biogas produced from the bio-mechanization of plants is being utilized to light the street lights in that locality.
Biogas is produced in the absence of oxygen or an anaerobic environment, due to the decomposition of organic material through certain bacteria. The whole process is referred as anaerobic digestion because biological decomposition takes place in a reactor, where bacteria produce biogas. This biomass can stay in the reactor for about 2–3 weeks. In the end, the by-product produced in this process is a solid residue that is high-grade manure. Generally, in the biogas plant, biomass like vegetable wastes and animal excreta undergo decomposition in the absence of oxygen and form a mixture of gasses. Biogas consists of about 2/3 methane (CH4), 1/3 carbon dioxide (CO2), a little hydrogen sulfide (H2S), and a low hydrogen (H2). It is created by the decomposition of manure and other forms of organic waste from households or industries in anaerobic tanks where it is heated (Figure 12). The biogas is used for cooking and lighting purposes.
Biomethanation process.
The following are the disadvantages of anaerobic processing:
In a large industrial scale, this process is not very economical compared to biofuel.
It is challenging to increase the efficiency of biogas systems.
The gasses come out from biogas as impurities, which are corrosive to the metal parts of internal combustion engines.
Not feasible to locate at all the locations.
RDF plants are in the initial stage of development in India. In this process, plenty of combustible components of MSW, such as plastic, cardboard, paper, and biodegradable waste, are converted into fuel pellets. It mainly involves drying, separation of combustion from MSW, size reduction, and pelletization after mixing with binder and additives as required. If MSW contains 35–40% moisture content, then it involves air-drying for 2 days. Then the waste is spread, and manual inspection is carried out to remove large size debris, tires, tree stones, tree trunk, etc. The air-dried MSW is fed uniformly into a rotary drying system, i.e., hot air generation burning oversized wastes. 10–12% moisture content is suitable to be maintained in MSW for densifying into fuel pellets.
After air-drying, MSW is passed through screening equipment (below 8 mm) to separate heavier combustibles and ferrous materials; it may cause harm to process equipment. Fine fraction contains organic matter, and it is already proven to be useful as garden manure.
Air-dried waste is then passed through the density separator; here light combustibles and an air barrier separate dense fractions (e.g., stones, glass, etc.). Parallel MSW is passed over a magnetic separation unit to remove magnetic materials. The binder and additives are mixed with ground solid waste in the mixer before pelletizing. Once pellets are coming out from the pelletizer, they are cooled and stored for dispatch. The RDF pellets are used as a coal substitute at a lower price.
In plasma gasification, the process converts all types of wastes into a synthesis gas composed of hydrogen, nitrogen, carbon monoxide, and water. This synthesis gas can be used to generate electrical power and useful liquid fuel, such as ethanol.
The following are the advantages of plasma gasification:
No segregation of MSW needed.
Waste to energy.
A 120 megawatt (MW) facility will require, on average, consuming 3000 tons of garbage per day (Mt garbage/d).
The plasma system can be retrofit on the existing power-generating plants, reducing time, and greenhouse gas emission.
Plasma systems can use old landfills, thoroughly cleaning up and beautifying our landscape.
Plasma gasification is an affordable, cost-effective solution compared to other alternative energy solutions.
The following are the disadvantages of the plasma gasification:
Lack of regulations needed for permits
Financial risk
Technical risk
Economics
In this section are some of the options available for waste disposal. However, it should be noted that the option selected for waste disposal must mesh with the existing sociocultural milieu, infrastructure, etc.
It is a thermal process for burning highly combustible waste like plastics, cardboard, paper, and rubber and combustible wastes like cartoons, wood scrap, floor sweepings, and food wastes at a very high temperature. The method does not apply to Indian conditions due to high dust and ash content of wastes; high capital costs, especially for adequate control of emissions; high operation and maintenance costs; and the need for skilled human resources. However, incineration is also associated with the production and release of carcinogenic and toxic compounds. Therefore, the incineration process is not environmentally friendly and is hence usually not recommended as a solid waste disposal technique.
Pyrolysis is also known as thermal pyrolysis. The combustion process is highly exothermic (releasing heat on burning in the presence of oxygen) in nature, whereas pyrolysis is highly endothermic (consuming flame) in the environment. Hence, the process of pyrolysis is known as destructive distillation. In this method, the solid wastes are heated under anaerobic conditions (i.e., burning without oxygen). The organic components of the solid wastes split up into volatile liquid and gaseous fractions (CO, CO2, CH4, tar, charred carbon). Pyrolysis cannot handle a wide variety of wastes that exists and will only have a small impact on the overall processing of waste.
Transportation is a necessary function for solid waste management activities since municipal solid waste, recyclables, yard waste, and other materials must be collected and transported to be managed. There are various methods for collecting and transporting waste, the choice of which depends on the type of solid waste, the source of solid waste, and the proper management method used.
A vital component of a reliable and well-run solid waste management system is to set up an efficient sanitary landfill and customer-responsive collection and disposal of solid waste. Waste collection services are provided to residents in all cities, either private or self-government agencies. In the meantime, the rapid increase in disposal costs across the city, the cost of collection, and the transfer of wastes continue to raise disposal as a percentage of overall service costs for most communities.
For collection and transfer, waste systems are often complex and challenging to design, because several factors must be considered and a wide range of collection and transfer options are available. The community participation is essential for an efficient MSWM system. However, the municipal authorities have failed to mobilize the community and educate citizens on the principles of handling waste and proper practices of storing it in their bins at the shop, household, etc. Due to the lack of an essential facility of collection of waste from source, citizens are likely to dump waste on the streets, open spaces, drains, and water bodies in the vicinity, creating insanitary conditions. Later, the pourakarmikas will collect the discharged waste through street sweeping, drain cleaning, etc. Street sweeping has thus become the principal method of primary collection.
The tools used for street sweeping are inefficient and outdated. For instance, the broom with a short handle is still in use, forcing sweepers to bend for hours, resulting in fatigue and loss of productivity.
Transportation of waste from the waste storage depots to the disposal site is done through a variety of vehicles, such as three-wheelers, tractors, and trucks. Most of the transport vehicles are old and open. They are usually loaded manually. Due to inadequate workshop facilities and maintenance procedures, the vehicles are poorly maintained. This leads to the breaking down of vehicles, resulting in failure of services for a long time.
The various technological options available for processing, treatment, and disposal of MSW are composting, vermicomposting, AD, incineration, gasification and pyrolysis, production of RDF, and sanitary landfilling.
The main benefits of composting include improvement in soil texture and augmenting of micronutrient deficiencies. It also increases the moisture-holding capacity of the soil and helps in maintaining soil health. However, it is an age-old concept for recycling nutrients to the soil. It does not require significant capital investment compared to other waste treatment options.
The technology of waste-to-energy projects and its viability and sustainability have been proven worldwide. WTE projects involve higher capital investment and are more complicated than other options of waste disposal. These plants are financially viable in developed countries mainly because of the tipping fees charged by the facility for the service of waste disposal, in addition to its revenue income from power sales. However, at present, in Bangalore, revenue from power sales is the only source of income for WTE plants. Technologically, it is feasible to set up even smaller capacity projects of the 1–5 MW range, corresponding to around 100–500 metric tons per day waste treatment. The significant role in making a WTE facility financially viable is the segregation of waste at the source to avoid the mixing of undesirable waste streams.
MSW landfills are essential in modern-day society because the segregation and disposal of solid waste materials into decentralized locations helps to minimize risks to public health and safety. Currently, in Bengaluru, MSW landfills remain open for decades before undergoing closure and post-closure phases, during which steps are taken to minimize the risk of environmental contamination. Although MSW landfills are an essential part of everyday living, they may present long-term threats to surface water and also hydrologically interlinked groundwater bodies. The impact of leachate on groundwater and surface water bodies has attracted much attention because of its enormous environmental significance. In the olden days, landfills were constructed without leachate collection systems and liners. Once leachate enters the groundwater, it will migrate downward through the unsaturated zone until it finally reaches the saturated area. This resulted in creating significant leachate-contaminate groundwater plumes that follow the hydraulic gradient of the groundwater system.
Unscientific management of MSW leachate will lead to contamination of the soil and water bodies. The presence of a contaminant in the soil can change the engineering properties of the soil. The leachate potential to contaminate the soils and groundwater and surface water bodies assumes significance in the context of existing MSW practices, which have many drawbacks.
Considering the importance of the problem discussed, this research mainly focuses on the characteristics of leachate generated from municipal solid waste landfill sites and its effect on surrounding water bodies near the Mavallipura landfill area in Bengaluru.
Many cities, including Bengaluru, are also facing the problems due to old dump yards situated close to the expanding cities. These dump yards need to be reclaimed for the growing needs of the city infrastructure development. This is the case for many cities in India with an alarming rate of the urbanization process. Also, they create a nuisance in the town, and the same needs to be stabilized or reclaimed. The waste sites that were earlier in the periphery of the corporation limits of Bengaluru City are now in the development zone of a more magnificent Bengaluru City, as shown in Figure 13. It can be seen from a satellite image that the built-up area has come near and around the earlier dump sites. Thus, it is clear that most of the old dumpsite which is existing around Bengaluru has become potential places for development.
Location of unauthorized dump sites in and around Bangalore City (Chanakya et al., 2011).
These structures built on these dumpsites can undergo distress due to the high settlement and cause failures due to the low strength of the dumped waste. These dumps can also cause groundwater contamination due to leaching of waste by the percolation of rainwater (Figures 14–16).
Unauthorized dumping along with wards in and around Bangalore City.
Major arterial and sub-arterial road network along with dump sites in and around Bangalore City.
Satellite image of built-up area along with dump sites in Greater Bangalore.
Naveen [12] carried out a detailed experimental program to study the variations in geotechnical properties for different wastes with a time of dump yard, i.e., in turn with the age of the waste. The data provided on geotechnical properties of the waste with varying degrees of decomposition helps to plan for the reclamation of waste dumps.
The objective of the reclamation is to return the MSW landfill to a condition as close as possible to leave the site in a state compatible with the surrounding ground. MSW landfill reclamation is a new approach used to expand the MSW landfill capacity and minimize the cost of acquiring additional land. The significant factors influencing the success of reclamation include chemical, hydrologic, and physical conditions of the fill materials, climate, availability of suitable plant species, and proper management of reclaimed sites [13].
The essential benefits may include a reduction in closure costs and reclamation of land for other purposes and recovered materials such as recyclables, soil, and waste, which can be burned as fuel. Some drawbacks exist in MSW landfill reclamation. This technology may release methane and other gasses from decomposing solid wastes. Also, the excavation work process involved in reclamation may cause adjacent landfill areas to collapse/sink. Hence, it is necessary to conduct a site characterization study. The site characterization should assess facility aspects, such as geotechnical and geological features and the stability of the surrounding area and identified groundwater, and determine the fractions of good soil, recyclable waste materials, and hazardous waste at the site [14]. Based on the available information from the site characterization, it provides project planners with a basis for assessing the potential economic benefits of a landfill reclamation project.
The economic benefits associated with landfill reclamation are indirect; they may include the following: increased disposal capacity; avoided or reduced cost of landfill closure; revenues from recyclable and reusable materials like ferrous metals, aluminum, plastic, and glass; combustion waste sold as fuel; reclaimed soil used as cover; and land value of sites reclaimed for other uses.
Geotechnical properties of municipal solid waste presume great importance in their reuse, disposal, as well as reclamation of waste and dump sites. Because of the high demand for land, the abandoned, closed landfills have to reclaim to meet the growing needs of the society. Due to several reasons, the population around the improperly operated landfills is demanding the closure of the landfill. However, just leaving the landfill without proper closure cannot be allowed. Thus any attempt to reclaim land for development should come after the characterization of waste for their physical and chemical composition and geotechnical properties. Therefore, these studies constitute the first step to successfully implementing a comprehensive waste management system.
Based on the above context, the following conclusions can be drawn:
For setup, the WTE plants require higher capital investment and are more complicated than the other options of waste disposal.
WTE plants are suitable in developed countries mainly because of the tipping fees/gate fees charged by the facility for the service of waste disposal, in addition to its revenue income from power sales.
Due to the high content of biodegradable waste in Bengaluru, a biological process is needed such as anaerobic digestion and composting to treat the waste, gasification, and pyrolysis.
Plasma gasification technology can reduce the need for landfills; it can create more renewable energy than the projected energy from solar, wind, landfill gas, and geothermal energies combined.
RDF plants are in the initial stage of development in India. It is beneficial in preparing an enriched fuel feed for thermal processes like incineration. The RDF pellets are used as a coal substitute at a lower price.
The pyrolysis process cannot handle the wide variety of wastes, and the end products of pyrolysis are carbon black oil that can be resent to a refiner and hydrocarbon gasses that can be used to make electricity or stream.
Sanitary landfill is the cheapest, simplest, and most cost-effective method for disposing of waste.
Mesenchymal stromal cells (MSCs) are progenitors of connective tissues, initially characterized as plastic adherent, fibroblastic cells, with the potential to differentiate into many types of cells, including predominantly osteoblasts (cells that secrete the matrix of the bones), chondrocytes (cells embedded in the lacunae of the cartilage matrix), and adipocytes (fat-storing cells), under appropriate conditions. MSC studies have progressed rapidly since the initial report of human MSC isolation from bone marrow. MSCs have been shown to reside within the connective tissues of most organs. Owing to their ease of isolation and unique characteristics, MSCs have been widely regarded as potential candidates for tissue engineering and repair. Further, the fate decision of MSCs has also piqued the interest of scientists. During the last two decades, various signaling molecules important to MSC differentiation have been identified, and the epigenetic regulation of MSC differentiation has recently become a research hotspot.
The transformation process of MSCs from a self-renewing state to a specific lineage is always accompanied by changes in cell morphology and function, which are largely determined by the differential expression of genes. Specifically, genes related to self-renewal are turned off, and transcription of cell type-specific genes is activated. Epigenetic regulation refers to the phenotypic change through gene differential expression without DNA sequence alteration, including four main categories: [1] DNA methylation, [2] histone modifications, and [3] chromatin remodeling (nucleosome positioning); and [4] non-coding RNAs. It has been widely reported that epigenetic and post-translational modifications have a broad and far-reaching influence on MSC differentiation at multiple levels. Here, we provide an overview of the recent findings regarding the roles of epigenetic modification in the fate decision of MSCs.
DNA methylation is an important epigenetic modification referring to the addition of a methyl (-CH3) group to the fifth carbon atom of a cytosine ring to form 5-methylcytosine (5-mC). The process is catalyzed by enzymes known as DNA methyltransferases (DNMTs). DNA methylation was the first epigenetic mark to be discovered, and it plays an important role in normal human growth, development, aging, tumorigenesis, and other genetic and epigenetic diseases. This epigenetic mark has the ability to turn genes on or off and can be inherited through cell division. Recent studies have suggested that methylation and demethylation of specific genes, such as
According to numerous studies, DNA methylation is dynamically involved in the osteogenesis of MSCs. Generally, it may be considered that DNA methylation has a repressor role in the promoter regions with CpG islands, blocking gene expression. During osteogenic differentiation, demethylation was observed at specific CpG regions in the promoters of osteogenic lineage-specific genes, such as
Villagra
Adipogenesis is highly regulated by a sequential cascade of transcriptional events. Key transcriptional factors controlling adipogenesis include several CCAAT/enhancer-binding protein (C/EBP) family members, including C/EBPα, β, and δ, and the nuclear receptor peroxisome proliferator γ (PPARγ). On the other hand, a number of negative transcriptional factors have also been identified, including GATA2/3, chicken ovalbumin upstream promoter transcription factor (COUP-TF), interferon regulatory factors (IRFs), and Wnt family proteins.
Barrand
DNA methylation and demethylation status also influence MSC chondrogenic differentiation. DNA methylation at specific CpGs has been shown to influence genes such as
Histone modification, a common form of epigenetic regulation, refers to post-translational modifications that are added to the N-terminal tail of histones. Histone modification has been shown to play important roles in regulating cell-specific gene expression. So far, more than sixty different residues on core histones (H2A, H2B, H3, H4) with potential to be modified have been reported. These modifications made to histones, including acylation, methylation, phosphorylation, ubiquitination, and sumoylation, can impact gene expression by altering the chromatin structure or recruiting histone modifiers. Histone proteins function to package DNA, which wraps around the eight histones, into chromosomes. In general, it has been well established that histones on the promoter regions of master transcription factors associated with MSC cell fate commitment, such as
Involved epigenetic histone modification | Factor | Mechanism | Result | Reference |
---|---|---|---|---|
Histone deacetylation | HDAC inhibitor | Stimulate the transcription of p21CIP1/WAF1 through enhancing the H3 and H4 acetylation | Arrest the cell cycle at the G2/M check point, inhibit adipogenic, chondrogenic, and neurogenic differentiation; promote osteogenesis | [18] |
Histone acetylation | Knockdown of PCAF (histone H3K9 acetyltransferase) | Insufficient to increase H3K9 acetylation at promoters of BMP2, BMP4, BMPR2B, and Runx2 | INHIBIT adipogenic differentiation and promote osteogenic differentiation in MSCs; reduce the bone formation both in vitro and in vivo | [19] |
Histone acetylation | GCN5 knockdown | Insufficient to inhibit NF-κB signaling by mediating the proteasomal degradation of p65 (acetyl K310) | Inhibits osteogenic differentiation of MSCs | [20] |
Histone deacetylation | SIRT1 knockout | Insufficient to deacetylate β-catenin to promote its accumulation in the nucleus | Reduce differentiation towards osteoblasts, and chondrocytes | [21] |
Histone demethylation | Overexpression of KDM5A | Decrease H3K4me3 levels on promoters of Runx2 by demethylating H3K4me3 | Inhibit osteogeninsis; lead to osteoporosis | [22] |
Histone methylation | G9a inhibitor | Unclear (correlate with PPARγ and C/EBPα expression) | Impair the proliferation but the anti-proliferative effect is not sustained; increase adipogenic potential and decrease osteogenic potential of MSCs | [23] |
Histone methylation | Downregulation of BMI1 | Insufficient to recruit and stabilize PRC2 which trimethylate H3K27 | Cellular senescence | [24] |
Histone methylation | Downregulation of EZH2 | Insufficient to trimethylate H3K27 as catalytic subunit of PRC2 and keep a high extent of H3K27me3 to suppress p16INK4A-induced senescence | Cellular senescence | [24] |
Histone modification in MSC differentiation and aging.
Histone acetylation is an epigenetic modification characterized by the addition of an acetyl group (COCH3) to histone proteins, specifically to lysine residues within the N-terminal tail. Histone acetylation is one of the most common epigenetic modifications, which leads to the neutralization of the positive charge on the histone proteins, weakening their interaction with DNA, and finally promoting the opening of chromatin structure and activating gene transcription. On the other hand, histone deacetylation is related to chromatin transcription inhibition. The level of histone acetylation is mainly regulated by histone acetylase (HAT) and histone deacetylase (HDAC).
The degree of histone acetylation of related regulatory genes can reflect the maintenance of stemness and the differentiation status of MSCs. During the process of osteogenic differentiation, the expression of osteogenic genes (such as
Histone methylation is another common post-translational modification by which methyl groups are transferred to the amino acids of histone proteins that make up the nucleosomes. Histone methylation can occur at various sites in histone proteins, primarily on lysine and arginine residues, and it can be governed by multiple positive and negative regulators, even at a single site, to either activate or repress transcription. Histone methylation is regulated by histone methyltransferase (HMT) and histone demethylase (HDM), which can be monomethylated, dimethylated, or trimethylated.
The increase in methylation usually promotes the affinity of histones to DNA and increases the degree of transcriptional inhibition, such as H3K9 methylation and H3K27 methylation. H3K9 dimethylation and trimethylation are typical repressive histone modifications that mediate the formation of heterochromatic regions. It was reported that the knockdown of ESET, a H3K9 methyltransferase, causes an aberrant expression of Runx2 and finally leads to the impairment of osteogenic differentiation and bone defects in mice. On the other hand, the knockdown of EHMT1, a H3K9 specific methyltransferase, resulted in decreased H3K9me2 levels on the promoters of Runx2, thereby upregulating transcription in mouse tissues. With respect to the adipogenic differentiation of MSCs, it was found that the enrichment of H3K9me1 and H3K9me2 on the promoters of C/EBP and PPARγ was negatively associated with adipogenic differentiation. Lowering the H3K9 methylation levels in these regions by either H3K9 demethylase or HMT inhibitors ultimately promoted adipogenic differentiation. In addition, H3K9me3 levels in the promoter region of Sox9, as well as its target genes
The mechanisms of histones modification.
Chromatin remodeling is the dynamic modification of chromatin architecture, which is an important mechanism for regulating gene expression. In eukaryotes, DNA is tightly wound into a complex called chromatin. Chromatin remodeling allows the access of tightly condensed DNA to various regulatory factors, such as transcription factors and components of DNA replication, so that specific genes can be expressed. The basic mechanism of chromatin remodeling depends on the three dynamic properties of nucleosomes: reconstruction, enzyme-induced covalent modification, and repositioning. In addition, the aforementioned histone modification is another important aspect of chromatin remodeling. Aberrations in chromatin remodeling proteins are associated with various human disorders and diseases. The major activities involved in nucleosome structure alterations use the energy supplied by ATP hydrolysis to affect nucleosomes. These enzymes are called ATP-dependent chromatin (or nucleosome) remodeling factors. The system involves four subfamilies of ATP-dependent chromatin remodeling complexes, namely switch/sucrose non-fermentable (SWI/SNF), nucleosome remodeler deacetylase (NuRD), INO80, and imitation switch (ISWI).
Several studies have demonstrated that functional SWI/SNF machinery plays an important role in regulating MSC tri-lineage differentiation by interacting with tissue-specific transcription factors and crosstalk with cell signaling pathways. Brahma-associated factor (BAF) complex subunits have been implicated in MSC osteo-lineage commitment. For example, depletion of BRG1 leads to constitutive osteo-lineage gene expression [37]. BRM negatively regulates osteocalcin expression [38]. Loss of the classical BAF restricted subunit Pbrm1/Arid2/Brd7 leads to reduced osteogenesis without compromising adipogenesis [39]. It has also been reported that SWI/SNF-dependent chromatin remodeling is involved in MSC adipogenic differentiation. BRG1 overexpression was associated with promoted adipogenic differentiation, which was associated with a marked increase in the differentiation markers PPARγ and LPL [40]. BAF45A was identified as an important regulator of adipogenic differentiation in human MSCs [41]. In addition, other ATP-dependent chromatin remodelers, such as chromodomain helicase DNA binding (CHD) proteins, are also involved in MSC lineage commitment. CHD4 was reported to be implicated in chondrogenesis. Simon
The RNA world is divided into two classes: 1) RNAs that have coding potential (mRNAs) and 2) RNAs without coding potential, referred to as non-coding RNAs (ncRNAs). Although mRNAs have been extensively studied, ncRNAs span more than 98% of DNA transcripts. In the past, these molecules were considered as “evolutionary junk” but increasing evidence suggests that these molecules spatiotemporally regulate protein-coding gene expression in several molecular mechanisms. With improved RNA-sequencing techniques, in recent years, there have been great advances in identifying and understanding ncRNAs. Epigenetic ncRNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), piwi-interacting RNA (piRNA), and long noncoding RNAs (lncRNA), have been reported to play key roles in the regulation of various diseases and biological processes, including cellular differentiation, proliferation, apoptosis, gene regulation, and cancer development.
lncRNA is a novel class of noncoding RNAs longer than 200 nt, which can regulate gene expression at the transcriptional and post-transcriptional levels. LncRNAs are mainly located in the cell nucleus or cytoplasm, affecting the status and fate of cells through different post-transcriptional mechanisms. Nuclear lncRNAs guide chromatin modifiers, such as DNA methyltransferase, histone methyltransferase, and heteronuclear ribosome protein, to a specific genetic locus and induce chromatin structure remodeling, which in turn regulates gene expression either positively or negatively. Cytoplasmic lncRNAs can either block the functional site or alter the structure and modification of specific proteins, thereby regulating the function and stabilization of these proteins, and ultimately alter the fate and function of cells. During the last decade, multiple studies have demonstrated that lncRNAs are widely involved in growth and development by controlling the fate of cells, including MSCs.
Studies have demonstrated the importance of lncRNAs in bone regeneration and bone formation. Many lncRNAs regulating the osteogenic differentiation of MSCs have been identified, including ANCR, AK141205, AK028326, DANCR, MALAT1, MEG3, MORD, and POIR; these either promote or inhibit osteogenic differentiation through diverse pathways. For example, MALAT1 promotes
MicroRNAs are the most abundant class of small ncRNAs with a length of 21–25 nt, and have been studied extensively. miRNAs are also involved in the epigenetic regulation of genes in both the cytoplasm and nucleus through different mechanisms. Their main action is the negative regulation of gene expression by specifically binding to a target mRNA through base complementary pairing and inducing its degradation or the inhibition of its translation.
Accumulating evidence indicates that miRNAs play an important role in the maintenance of stemness and differentiation of MSCs (Table 2). As mentioned above, lineage differentiation of MSCs is a complex biological process. For example, MSCs differentiate into osteogenic progenitor cells and subsequently osteoblasts, and then gradually become mature bone cells along with a variety of extracellular matrix mineralization. This process involves a large number of secretory and transcription factors. In addition, the differentiation and maturation of MSCs also involves signaling pathways such as WNT, BMP, and PI3K/Akt. The key effector molecules in these pathways can be regulated by miRNAs, which in turn affects MSC fate decisions. Recently, various miRNAs, including miR-20b, -29b, -30a-5p, -142-3p, -196a, -210, -746-5p, -2861, -3960, -335-5p, etc., have been reported to enhance osteogenic differentiation, whereas miR -23a, -26a, -30c, -34b, -34c, -125, -133a, -135a, -137, -141, -148, -200a, -204, -205, -206, -217, and -338 could impede osteogenic differentiation, and miR-143, -24, -31, -30c, and -642a-3p are involved in regulating adipogenesis. Oskowitz
Involved miRNA | Mechanism | Result | Reference |
---|---|---|---|
miR-23a | targets LRP5 and subsequently suppress the Wnt/β-catenin signaling pathway | Inhibit osteogenesis of MSCs | [51] |
miR-26a | in BMSCs: targets GSK3 in ADSCs: targets Smad1 mainly and inhibits BMP signaling pathway | Inhibit osteogenesis of ADSCs and promote osteogenesis of BMSCs | [52] |
miR-30c | reduces Runx2 protein | Inhibit osteogenesis of MSCs | [53] |
miR-34c | |||
miR-133a | |||
miR-135a | |||
miR-137 | |||
miR-204 | |||
miR-205 | |||
miR-217 | |||
miR-338 | |||
miR-20b | Activate the BMPs/Runx2 signaling pathway at four levels, which consists of repressing PPARγ, Bambi and Crim1 | Promote ostegenesis | [54, 55] |
miR-29b | activates the AKT/β-catenin signaling pathway by inhibiting PTEN expression | Promote osteogenesis of hADSCs | [56] |
miR-196a | targets HOXC8 (a negative regulator of SMAD1) | Inhibit proliferation and promote osteogenesis of hDASCs | [57] |
miR-17-5p | Represses the Wnt signaling pathway effector Tcf7l2 | Promote adipogenesis of BM-MSCs | [58, 59] |
miR-21 | Alters SMAD3 phosphorylation without affecting total levels of SMAD3 protein and modulate TGF-β signaling pathway | [59, 60] | |
miR-143 | Directly represses MAP2K5 (a key member of the MAPKK family in the MAPK signaling pathway) | [59, 61] | |
miR-30a | Targets Runx2 | Promote adipogenesis | [62] |
miR-30d | |||
miR-642a-3p | unknown | In a high level in adipogenesis | [62] |
miRNA and MSCs differentiation.
“Epigenetics” was first used to define the complex interactions between the genome and the environment that are involved in the development and differentiation of organisms. Nowadays, the term refers to heritable alterations in gene expression that are not mediated at the DNA sequence level. Accumulating evidence has suggested that the processes of epigenetic modifications are crucial and largely responsible for the variable activation and repression of specific genes at specific time points during the lifespan of stem cells, allowing for the terminally differentiated phenotype. With the advances in biological and experimental technologies, a variety of epigenetic modifications involved in the cell fate determination of MSCs have been discovered in recent years. In addition to the types of epigenetic modifications introduced in the article, some researchers have suggested the role of histone phosphorylation, ubiquitination, and other modifications in the differentiation of MSCs. On this basis this information, drugs that effectively regulate these modifications have been developed to provide precise differentiation conditions for MSCs and make them more effective in clinical treatment. The disadvantage of epigenetic therapy using small molecule drugs is the lack of specificity, which needs to be further studied. In summary, epigenetic modifications play an important regulatory role in the cell fate determination of MSCs, but the precise function of these modifications in different MSC types, as well as the associated underlying mechanisms, remain to be thoroughly investigated. In-depth research in this field would provide important reference data for the differentiation mechanism research and clinical application of MSCs.
The authors were supported by the National Key Research and Development Program of China (2020YFA0113003, 2018YFC1004803) and the Fundamental Research Funds for the Central Universities.
The authors declare no competing financial interests.
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Consequently, knowledge of exoplanets is considerably more limited than Solar System planets. This chapter reviews the essential characteristics of Solar System planets and associated data derived from a variety of observational approaches. Exoplanet characteristics and their comparison to Solar System planets are provided as well as general detection methods and planned probes to gather additional data.",book:{id:"10210",slug:"solar-system-planets-and-exoplanets",title:"Solar System Planets and Exoplanets",fullTitle:"Solar System Planets and Exoplanets"},signatures:"Joseph Bevelacqua",authors:[{id:"115462",title:"Dr.",name:"Joseph",middleName:"John",surname:"Bevelacqua",slug:"joseph-bevelacqua",fullName:"Joseph Bevelacqua"}]},{id:"65725",title:"On the Deviation of the Lunar Center of Mass to the East: Two Possible Mechanisms Based on Evolution of the Orbit and Rounding Off the Shape of the Moon",slug:"on-the-deviation-of-the-lunar-center-of-mass-to-the-east-two-possible-mechanisms-based-on-evolution-",totalDownloads:1025,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"It is known that the Moon’s center of mass (COM) does not coincide with the geometric center of figure (COF) and the line “COF/COM” is not directed to the center of the Earth, but deviates from it to the South-East. Here, we discuss two mechanisms to explain the deviation of the lunar COM to the East from the mean direction to Earth. The first mechanism considers the secular evolution of the Moon’s orbit, using the effect of the preferred orientation of the satellite with synchronous rotation to the second (empty) orbital focus. It is established that only the scenario with an increase in the orbital eccentricity e leads to the required displacement of the lunar COM to the East. It is important that high-precision calculations confirm an increase e in our era. In order to fully explain the shift of the lunar COM to the East, a second mechanism was developed that takes into account the influence of tidal changes in the shape of the Moon at its gradual removal from the Earth. The second mechanism predicts that the elongation of the lunar figure in the early era was significant. As a result, it was found that the Moon could have been formed in the annular zone at a distance of 3–4 radii of the modern Earth.",book:{id:"8444",slug:"lunar-science",title:"Lunar Science",fullTitle:"Lunar Science"},signatures:"Boris P. Kondratyev",authors:[{id:"277909",title:"Prof.",name:"Boris",middleName:"Petrovich",surname:"Kondratyev",slug:"boris-kondratyev",fullName:"Boris Kondratyev"}]},{id:"68357",title:"Solar System Exploration Augmented by In Situ Resource Utilization: System Analyses, Vehicles, and Moon Bases for Saturn Exploration",slug:"solar-system-exploration-augmented-by-in-situ-resource-utilization-system-analyses-vehicles-and-moon",totalDownloads:853,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Human and robotic missions to Saturn are presented and analyzed with a range of propulsion options. Historical studies of space exploration, planetary spacecraft and astronomy, in situ resource utilization (ISRU), and industrialization all point to the vastness of natural resources in the solar system. Advanced propulsion is benefitted from these resources in many ways. While advanced propulsion systems were proposed in these historical studies, further investigation of nuclear options using high-power nuclear electric and nuclear pulse propulsion as well as advanced chemical propulsion can significantly enhance these scenarios. Updated analyses based on these historical visions are presented. At Saturn, nuclear pulse propulsion with alternate propellant feed systems and Saturn moon exploration with chemical propulsion and nuclear electric propulsion options are discussed. Issues with using in situ resource utilization on Saturn’s moons are discussed. At Saturn, the best locations for exploration and the use of the moons as central locations for Saturn moon exploration are assessed. Environmental issues on Titan’s surface may present extreme challenges for some ISRU processes. In-space bases for moon-orbiting propellant processing and ground-based processing will be assessed.",book:{id:"7338",slug:"planetology-future-explorations",title:"Planetology",fullTitle:"Planetology - Future Explorations"},signatures:"Bryan Palaszewski",authors:[{id:"279275",title:"M.Sc.",name:"Bryan",middleName:null,surname:"Palaszewski",slug:"bryan-palaszewski",fullName:"Bryan Palaszewski"}]},{id:"65534",title:"Solar System Exploration Augmented by In Situ Resource Utilization: Lunar Base Issues",slug:"solar-system-exploration-augmented-by-in-situ-resource-utilization-lunar-base-issues",totalDownloads:1131,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Creating a presence and an industrial capability on the Moon is essential for the development of humankind. There are many historical study results that have identified and quantified the lunar resources and analyzed the methods of obtaining and employing those resources. The idea of finding, obtaining, and using these materials is called in situ resource utilization (ISRU). The ISRU research and development efforts have led to new ideas in rocket propulsion. Applications in chemical propulsion, nuclear electric propulsion, and many other propulsion systems will be critical in making the initial lunar base and future lunar industries more sustainable and will lead to brilliant futures for humanity.",book:{id:"8444",slug:"lunar-science",title:"Lunar Science",fullTitle:"Lunar Science"},signatures:"Bryan Palaszewski",authors:[{id:"279275",title:"M.Sc.",name:"Bryan",middleName:null,surname:"Palaszewski",slug:"bryan-palaszewski",fullName:"Bryan Palaszewski"}]},{id:"32533",title:"Measuring the Isotopic Composition of Solar Wind Noble Gases",slug:"measuring-the-isotopic-composition-of-solar-wind-noble-gases",totalDownloads:2785,totalCrossrefCites:6,totalDimensionsCites:9,abstract:null,book:{id:"1617",slug:"exploring-the-solar-wind",title:"Exploring the Solar Wind",fullTitle:"Exploring the Solar Wind"},signatures:"Alex Meshik, Charles Hohenberg, Olga Pravdivtseva and Donald Burnett",authors:[{id:"114740",title:"Prof.",name:"Alexander",middleName:null,surname:"Meshik",slug:"alexander-meshik",fullName:"Alexander Meshik"},{id:"115300",title:"Prof.",name:"Donald",middleName:null,surname:"Burnett",slug:"donald-burnett",fullName:"Donald Burnett"},{id:"115301",title:"Prof.",name:"Charles",middleName:null,surname:"Hohenberg",slug:"charles-hohenberg",fullName:"Charles Hohenberg"},{id:"115302",title:"Dr.",name:"Olga",middleName:null,surname:"Pravdivtseva",slug:"olga-pravdivtseva",fullName:"Olga Pravdivtseva"}]}],onlineFirstChaptersFilter:{topicId:"98",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82332",title:"Access to Space, Access to the Moon – Two Sides of the Same Coin?",slug:"access-to-space-access-to-the-moon-two-sides-of-the-same-coin-",totalDownloads:13,totalDimensionsCites:0,doi:"10.5772/intechopen.105175",abstract:"The dynamics of human expansion towards space are going through Earth external layers, orbital space and the Moon. With its low gravity, slingshot effect relative to Earth, on-site resources and relative proximity to Earth in the solar system, the renewed space race is effectively returning first to the Moon. A psychological bridge to enlarge our civilization with a permanent bridge to our natural satellite. The development of this Earth-Moon system, requires enormous amount of finances, energy, science, technology, but over all, opportunities. This chapter deals with the efforts and the mental changes that may eventually result from all of these changes.",book:{id:"10955",title:"Lunar Science - Habitat and Humans",coverURL:"https://cdn.intechopen.com/books/images_new/10955.jpg"},signatures:"Yann-Henri Chemin"},{id:"81141",title:"Modeling Radiation Damage in Materials Relevant for Exploration and Settlement on the Moon",slug:"modeling-radiation-damage-in-materials-relevant-for-exploration-and-settlement-on-the-moon",totalDownloads:32,totalDimensionsCites:0,doi:"10.5772/intechopen.102808",abstract:"Understanding the effect of radiation on materials is fundamental for space exploration. Energetic charged particles impacting materials create electronic excitations, atomic displacements, and nuclear fragmentation. Monte Carlo particle transport simulations are the most common approach for modeling radiation damage in materials. However, radiation damage is a multiscale problem, both in time and in length, an aspect treated by the Monte Carlo simulations only to a limited extent. In this chapter, after introducing the Monte Carlo particle transport method, we present a multiscale approach to study different stages of radiation damage which allows for the synergy between the electronic and nuclear effects induced in materials. We focus on cumulative displacement effects induced by radiation below the regime of hadronic interactions. We then discuss selected studies of radiation damage in materials of importance and potential use for the exploration and settlement on the Moon, ranging from semiconductors to alloys and from polymers to the natural regolith. Additionally, we overview some of the novel materials with outstanding properties, such as low weight, increased radiation resistance, and self-healing capabilities with a potential to reduce mission costs and improve prospects for extended human exploration of extraterrestrial bodies.",book:{id:"10955",title:"Lunar Science - Habitat and Humans",coverURL:"https://cdn.intechopen.com/books/images_new/10955.jpg"},signatures:"Natalia E. Koval, Bin Gu, Daniel Muñoz-Santiburcio and Fabiana Da Pieve"},{id:"80241",title:"The Evolution of the Moon’s Orbit Over 100 Million Years and Prospects for the Research in the Moon",slug:"the-evolution-of-the-moon-s-orbit-over-100-million-years-and-prospects-for-the-research-in-the-moon",totalDownloads:65,totalDimensionsCites:0,doi:"10.5772/intechopen.102392",abstract:"As a result of solving the problem of interaction of Solar-system bodies, data on the evolution of the Moon’s orbit were obtained. These data were used as the basis for the development of a mathematical model for the Moon representing its motion over an interval of 100 million years. A program of exploration of the Moon with the aim of creating a permanent base on it is outlined. Such a base is intended for exploring the Earth, the Sun, and outer space.",book:{id:"10955",title:"Lunar Science - Habitat and Humans",coverURL:"https://cdn.intechopen.com/books/images_new/10955.jpg"},signatures:"Joseph J. Smulsky"},{id:"80217",title:"Educational and Scientific Analog Space Missions",slug:"educational-and-scientific-analog-space-missions",totalDownloads:87,totalDimensionsCites:0,doi:"10.5772/intechopen.101392",abstract:"Analog space missions in Poland include international scientific, technological, and business projects designed and realized by a private research company Analog Astronaut Training Center Ltd. (AATC) devoted to the future Moon and Mars exploration. Growing experience in educational aspect of the training as well as continuous development of the habitat and its professional space science laboratory equipment correspond to increased interest of educational organizations, universities, and individual students. We serve unique practical platform for space engineering, space master, and even space doctoral theses. In addition to a wide range of training courses offered for future astronauts, for example, diving, skydiving, rocket workshops, and stratospheric missions, AATC provides a private laboratory to simulate the space environment. It carries out scientific experiments focused on biology and space medicine, as well as addressing several multidisciplinary issues related to the Moon and Mars exploration, including space mining. The main goal of each our analog simulation is to get publishable results, what means that our analog astronauts obtain not only certification of completion of the training but also ability to continue studies and to perform it individually. This chapter summarizes methodology used by us, didactic tools, and obtained results for both educational and scientific analog simulations.",book:{id:"10955",title:"Lunar Science - Habitat and Humans",coverURL:"https://cdn.intechopen.com/books/images_new/10955.jpg"},signatures:"Agata Maria Kołodziejczyk and M. Harasymczuk"},{id:"79544",title:"Regolith and Radiation: The Cosmic Battle",slug:"regolith-and-radiation-the-cosmic-battle",totalDownloads:126,totalDimensionsCites:0,doi:"10.5772/intechopen.101437",abstract:"This chapter discusses regolith utilization in habitat construction mainly from the point of view of radiation protection of humans on missions of long duration. It also considers other key properties such as structural robustness, thermal insulation, and micrometeoroid protection that all have to be considered in parallel when proposing regolith-based solutions. The biological hazards of radiation exposure on the Moon are presented and put in the context of lunar exploration-type missions and current astronaut career dose limits. These factors guide the research in radiation protection done with lunar regolith simulants, which are used in research and development activities on Earth due to the reduced accessibility of returned lunar samples. The ways in which regolith can be used in construction influence its protective properties. Areal density, which plays a key role in the radiation shielding capacity of a given material, can be optimized through different regolith processing techniques. At the same time, density will also affect other important properties of the construction, e.g. thermal insulation. 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He is also active in popular science writing data science stories for reputed blogs (KDNuggets, TowardsDataScience, Naukas). 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He is an Associate Professor at the Department of Biomedical Engineering at Izmir Katip Celebi University, Izmir / Turkey, since 2019. In addition to academics, he has also founded Islerya Medical and Information Technologies Company, Izmir / Turkey, since 2017.\n\nHis main research interests cover biomedical signal processing, pattern recognition, medical device design, programming, and embedded systems. He has many scientific papers and participated in several projects in these study fields. He was an IEEE Student Member (2009-2011) and IEEE Member (2011-2014) and has been IEEE Senior Member since 2014.",institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"339677",title:"Dr.",name:"Mrinmoy",middleName:null,surname:"Roy",slug:"mrinmoy-roy",fullName:"Mrinmoy Roy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/339677/images/16768_n.jpg",biography:"An accomplished Sales & Marketing professional with 12 years of cross-functional experience in well-known organisations such as CIPLA, LUPIN, GLENMARK, ASTRAZENECA across different segment of Sales & Marketing, International Business, Institutional Business, Product Management, Strategic Marketing of HIV, Oncology, Derma, Respiratory, Anti-Diabetic, Nutraceutical & Stomatological Product Portfolio and Generic as well as Chronic Critical Care Portfolio. A First Class MBA in International Business & Strategic Marketing, B.Pharm, D.Pharm, Google Certified Digital Marketing Professional. Qualified PhD Candidate in Operations and Management with special focus on Artificial Intelligence and Machine Learning adoption, analysis and use in Healthcare, Hospital & Pharma Domain. Seasoned with diverse therapy area of Pharmaceutical Sales & Marketing ranging from generating revenue through generating prescriptions, launching new products, and making them big brands with continuous strategy execution at the Physician and Patients level. Moved from Sales to Marketing and Business Development for 3.5 years in South East Asian Market operating from Manila, Philippines. Came back to India and handled and developed Brands such as Gluconorm, Lupisulin, Supracal, Absolut Woman, Hemozink, Fabiflu (For COVID 19), and many more. In my previous assignment I used to develop and execute strategies on Sales & Marketing, Commercialization & Business Development for Institution and Corporate Hospital Business portfolio of Oncology Therapy Area for AstraZeneca Pharma India Ltd. Being a Research Scholar and Student of ‘Operations Research & Management: Artificial Intelligence’ I published several pioneer research papers and book chapters on the same in Internationally reputed journals and Books indexed in Scopus, Springer and Ei Compendex, Google Scholar etc. Currently, I am launching PGDM Pharmaceutical Management Program in IIHMR Bangalore and spearheading the course curriculum and structure of the same. I am interested in Collaboration for Healthcare Innovation, Pharma AI Innovation, Future trend in Marketing and Management with incubation on Healthcare, Healthcare IT startups, AI-ML Modelling and Healthcare Algorithm based training module development. I am also an affiliated member of the Institute of Management Consultant of India, looking forward to Healthcare, Healthcare IT and Innovation, Pharma and Hospital Management Consulting works.",institutionString:null,institution:{name:"Lovely Professional University",country:{name:"India"}}},{id:"310576",title:"Prof.",name:"Erick Giovani",middleName:null,surname:"Sperandio Nascimento",slug:"erick-giovani-sperandio-nascimento",fullName:"Erick Giovani Sperandio Nascimento",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y00002pDKxDQAW/ProfilePicture%202022-06-20%2019%3A57%3A24.788",biography:"Prof. Erick Sperandio is the Lead Researcher and professor of Artificial Intelligence (AI) at SENAI CIMATEC, Bahia, Brazil, also working with Computational Modeling (CM) and HPC. He holds a PhD in Environmental Engineering in the area of Atmospheric Computational Modeling, a Master in Informatics in the field of Computational Intelligence and Graduated in Computer Science from UFES. He currently coordinates, leads and participates in R&D projects in the areas of AI, computational modeling and supercomputing applied to different areas such as Oil and Gas, Health, Advanced Manufacturing, Renewable Energies and Atmospheric Sciences, advising undergraduate, master's and doctoral students. He is the Lead Researcher at SENAI CIMATEC's Reference Center on Artificial Intelligence. In addition, he is a Certified Instructor and University Ambassador of the NVIDIA Deep Learning Institute (DLI) in the areas of Deep Learning, Computer Vision, Natural Language Processing and Recommender Systems, and Principal Investigator of the NVIDIA/CIMATEC AI Joint Lab, the first in Latin America within the NVIDIA AI Technology Center (NVAITC) worldwide program. He also works as a researcher at the Supercomputing Center for Industrial Innovation (CS2i) and at the SENAI Institute of Innovation for Automation (ISI Automação), both from SENAI CIMATEC. He is a member and vice-coordinator of the Basic Board of Scientific-Technological Advice and Evaluation, in the area of Innovation, of the Foundation for Research Support of the State of Bahia (FAPESB). He serves as Technology Transfer Coordinator and one of the Principal Investigators at the National Applied Research Center in Artificial Intelligence (CPA-IA) of SENAI CIMATEC, focusing on Industry, being one of the six CPA-IA in Brazil approved by MCTI / FAPESP / CGI.br. He also participates as one of the representatives of Brazil in the BRICS Innovation Collaboration Working Group on HPC, ICT and AI. He is the coordinator of the Work Group of the Axis 5 - Workforce and Training - of the Brazilian Strategy for Artificial Intelligence (EBIA), and member of the MCTI/EMBRAPII AI Innovation Network Training Committee. He is the coordinator, by SENAI CIMATEC, of the Artificial Intelligence Reference Network of the State of Bahia (REDE BAH.IA). He leads the working group of experts representing Brazil in the Global Partnership on Artificial Intelligence (GPAI), on the theme \"AI and the Pandemic Response\".",institutionString:"Manufacturing and Technology Integrated Campus – SENAI CIMATEC",institution:null},{id:"1063",title:"Prof.",name:"Constantin",middleName:null,surname:"Volosencu",slug:"constantin-volosencu",fullName:"Constantin Volosencu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/1063/images/system/1063.png",biography:"Prof. Dr. Constantin Voloşencu graduated as an engineer from\nPolitehnica University of Timișoara, Romania, where he also\nobtained a doctorate degree. He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:'"Politechnica" University Timişoara',institution:null},{id:"221364",title:"Dr.",name:"Eneko",middleName:null,surname:"Osaba",slug:"eneko-osaba",fullName:"Eneko Osaba",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/221364/images/system/221364.jpg",biography:"Dr. Eneko Osaba works at TECNALIA as a senior researcher. He obtained his Ph.D. in Artificial Intelligence in 2015. He has participated in more than twenty-five local and European research projects, and in the publication of more than 130 papers. He has performed several stays at universities in the United Kingdom, Italy, and Malta. Dr. Osaba has served as a program committee member in more than forty international conferences and participated in organizing activities in more than ten international conferences. He is a member of the editorial board of the International Journal of Artificial Intelligence, Data in Brief, and Journal of Advanced Transportation. He is also a guest editor for the Journal of Computational Science, Neurocomputing, Swarm, and Evolutionary Computation and IEEE ITS Magazine.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"275829",title:"Dr.",name:"Esther",middleName:null,surname:"Villar-Rodriguez",slug:"esther-villar-rodriguez",fullName:"Esther Villar-Rodriguez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/275829/images/system/275829.jpg",biography:"Dr. Esther Villar obtained a Ph.D. in Information and Communication Technologies from the University of Alcalá, Spain, in 2015. She obtained a degree in Computer Science from the University of Deusto, Spain, in 2010, and an MSc in Computer Languages and Systems from the National University of Distance Education, Spain, in 2012. Her areas of interest and knowledge include natural language processing (NLP), detection of impersonation in social networks, semantic web, and machine learning. Dr. Esther Villar made several contributions at conferences and publishing in various journals in those fields. Currently, she is working within the OPTIMA (Optimization Modeling & Analytics) business of TECNALIA’s ICT Division as a data scientist in projects related to the prediction and optimization of management and industrial processes (resource planning, energy efficiency, etc).",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"49813",title:"Dr.",name:"Javier",middleName:null,surname:"Del Ser",slug:"javier-del-ser",fullName:"Javier Del Ser",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49813/images/system/49813.png",biography:"Prof. Dr. Javier Del Ser received his first PhD in Telecommunication Engineering (Cum Laude) from the University of Navarra, Spain, in 2006, and a second PhD in Computational Intelligence (Summa Cum Laude) from the University of Alcala, Spain, in 2013. He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. He is a Senior Member of the IEEE, and a recipient of the Biscay Talent prize for his academic career.",institutionString:"Tecnalia Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"278948",title:"Dr.",name:"Carlos Pedro",middleName:null,surname:"Gonçalves",slug:"carlos-pedro-goncalves",fullName:"Carlos Pedro Gonçalves",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRcmyQAC/Profile_Picture_1564224512145",biography:'Carlos Pedro Gonçalves (PhD) is an Associate Professor at Lusophone University of Humanities and Technologies and a researcher on Complexity Sciences, Quantum Technologies, Artificial Intelligence, Strategic Studies, Studies in Intelligence and Security, FinTech and Financial Risk Modeling. He is also a progammer with programming experience in:\n\nA) Quantum Computing using Qiskit Python module and IBM Quantum Experience Platform, with software developed on the simulation of Quantum Artificial Neural Networks and Quantum Cybersecurity;\n\nB) Artificial Intelligence and Machine learning programming in Python;\n\nC) Artificial Intelligence, Multiagent Systems Modeling and System Dynamics Modeling in Netlogo, with models developed in the areas of Chaos Theory, Econophysics, Artificial Intelligence, Classical and Quantum Complex Systems Science, with the Econophysics models having been cited worldwide and incorporated in PhD programs by different Universities.\n\nReceived an Arctic Code Vault Contributor status by GitHub, due to having developed open source software preserved in the \\"Arctic Code Vault\\" for future generations (https://archiveprogram.github.com/arctic-vault/), with the Strategy Analyzer A.I. module for decision making support (based on his PhD thesis, used in his Classes on Decision Making and in Strategic Intelligence Consulting Activities) and QNeural Python Quantum Neural Network simulator also preserved in the \\"Arctic Code Vault\\", for access to these software modules see: https://github.com/cpgoncalves. He is also a peer reviewer with outsanding review status from Elsevier journals, including Physica A, Neurocomputing and Engineering Applications of Artificial Intelligence. Science CV available at: https://www.cienciavitae.pt//pt/8E1C-A8B3-78C5 and ORCID: https://orcid.org/0000-0002-0298-3974',institutionString:"University of Lisbon",institution:{name:"Universidade Lusófona",country:{name:"Portugal"}}},{id:"241400",title:"Prof.",name:"Mohammed",middleName:null,surname:"Bsiss",slug:"mohammed-bsiss",fullName:"Mohammed Bsiss",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/241400/images/8062_n.jpg",biography:null,institutionString:null,institution:null},{id:"276128",title:"Dr.",name:"Hira",middleName:null,surname:"Fatima",slug:"hira-fatima",fullName:"Hira Fatima",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/276128/images/14420_n.jpg",biography:"Dr. Hira Fatima\nAssistant Professor\nDepartment of Mathematics\nInstitute of Applied Science\nMangalayatan University, Aligarh\nMobile: no : 8532041179\nhirafatima2014@gmal.com\n\nDr. Hira Fatima has received his Ph.D. degree in pure Mathematics from Aligarh Muslim University, Aligarh India. Currently working as an Assistant Professor in the Department of Mathematics, Institute of Applied Science, Mangalayatan University, Aligarh. She taught so many courses of Mathematics of UG and PG level. Her research Area of Expertise is Functional Analysis & Sequence Spaces. She has been working on Ideal Convergence of double sequence. She has published 17 research papers in National and International Journals including Cogent Mathematics, Filomat, Journal of Intelligent and Fuzzy Systems, Advances in Difference Equations, Journal of Mathematical Analysis, Journal of Mathematical & Computer Science etc. She has also reviewed few research papers for the and international journals. She is a member of Indian Mathematical Society.",institutionString:null,institution:null},{id:"414880",title:"Dr.",name:"Maryam",middleName:null,surname:"Vatankhah",slug:"maryam-vatankhah",fullName:"Maryam Vatankhah",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Borough of Manhattan Community College",country:{name:"United States of America"}}},{id:"414879",title:"Prof.",name:"Mohammad-Reza",middleName:null,surname:"Akbarzadeh-Totonchi",slug:"mohammad-reza-akbarzadeh-totonchi",fullName:"Mohammad-Reza Akbarzadeh-Totonchi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Ferdowsi University of Mashhad",country:{name:"Iran"}}},{id:"414878",title:"Prof.",name:"Reza",middleName:null,surname:"Fazel-Rezai",slug:"reza-fazel-rezai",fullName:"Reza Fazel-Rezai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"American Public University System",country:{name:"United States of America"}}},{id:"426586",title:"Dr.",name:"Oladunni A.",middleName:null,surname:"Daramola",slug:"oladunni-a.-daramola",fullName:"Oladunni A. Daramola",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Federal University of Technology",country:{name:"Nigeria"}}},{id:"357014",title:"Prof.",name:"Leon",middleName:null,surname:"Bobrowski",slug:"leon-bobrowski",fullName:"Leon Bobrowski",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Bialystok University of Technology",country:{name:"Poland"}}},{id:"302698",title:"Dr.",name:"Yao",middleName:null,surname:"Shan",slug:"yao-shan",fullName:"Yao Shan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Dalian University of Technology",country:{name:"China"}}},{id:"354126",title:"Dr.",name:"Setiawan",middleName:null,surname:"Hadi",slug:"setiawan-hadi",fullName:"Setiawan Hadi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Padjadjaran University",country:{name:"Indonesia"}}},{id:"125911",title:"Prof.",name:"Jia-Ching",middleName:null,surname:"Wang",slug:"jia-ching-wang",fullName:"Jia-Ching Wang",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"National Central University",country:{name:"Taiwan"}}},{id:"332603",title:"Prof.",name:"Kumar S.",middleName:null,surname:"Ray",slug:"kumar-s.-ray",fullName:"Kumar S. Ray",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Statistical Institute",country:{name:"India"}}},{id:"415409",title:"Prof.",name:"Maghsoud",middleName:null,surname:"Amiri",slug:"maghsoud-amiri",fullName:"Maghsoud Amiri",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Allameh Tabataba'i University",country:{name:"Iran"}}},{id:"357085",title:"Mr.",name:"P. Mohan",middleName:null,surname:"Anand",slug:"p.-mohan-anand",fullName:"P. Mohan Anand",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"356696",title:"Ph.D. Student",name:"P.V.",middleName:null,surname:"Sai Charan",slug:"p.v.-sai-charan",fullName:"P.V. Sai Charan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"357086",title:"Prof.",name:"Sandeep K.",middleName:null,surname:"Shukla",slug:"sandeep-k.-shukla",fullName:"Sandeep K. Shukla",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}}]}},subseries:{item:{id:"9",type:"subseries",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering",keywords:"Biotechnology, Biosensors, Biomaterials, Tissue Engineering",scope:"The Biotechnology - Biosensors, Biomaterials and Tissue Engineering topic within the Biomedical Engineering Series aims to rapidly publish contributions on all aspects of biotechnology, biosensors, biomaterial and tissue engineering. We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics can include but are not limited to: Biotechnology such as biotechnological products and process engineering; Biotechnologically relevant enzymes and proteins; Bioenergy and biofuels; Applied genetics and molecular biotechnology; Genomics, transcriptomics, proteomics; Applied microbial and cell physiology; Environmental biotechnology; Methods and protocols. Moreover, topics in biosensor technology, like sensors that incorporate enzymes, antibodies, nucleic acids, whole cells, tissues and organelles, and other biological or biologically inspired components will be considered, and topics exploring transducers, including those based on electrochemical and optical piezoelectric, thermal, magnetic, and micromechanical elements. Chapters exploring biomaterial approaches such as polymer synthesis and characterization, drug and gene vector design, biocompatibility, immunology and toxicology, and self-assembly at the nanoscale, are welcome. Finally, the tissue engineering subcategory will support topics such as the fundamentals of stem cells and progenitor cells and their proliferation, differentiation, bioreactors for three-dimensional culture and studies of phenotypic changes, stem and progenitor cells, both short and long term, ex vivo and in vivo implantation both in preclinical models and also in clinical trials.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/9.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11405,editor:{id:"126286",title:"Dr.",name:"Luis",middleName:"Jesús",surname:"Villarreal-Gómez",slug:"luis-villarreal-gomez",fullName:"Luis Villarreal-Gómez",profilePictureURL:"https://mts.intechopen.com/storage/users/126286/images/system/126286.jpg",biography:"Dr. Luis Villarreal is a research professor from the Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana, Baja California, México. Dr. Villarreal is the editor in chief and founder of the Revista de Ciencias Tecnológicas (RECIT) (https://recit.uabc.mx/) and is a member of several editorial and reviewer boards for numerous international journals. He has published more than thirty international papers and reviewed more than ninety-two manuscripts. His research interests include biomaterials, nanomaterials, bioengineering, biosensors, drug delivery systems, and tissue engineering.",institutionString:null,institution:{name:"Autonomous University of Baja California",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null,series:{id:"7",title:"Biomedical Engineering",doi:"10.5772/intechopen.71985",issn:"2631-5343"},editorialBoard:[{id:"35539",title:"Dr.",name:"Cecilia",middleName:null,surname:"Cristea",slug:"cecilia-cristea",fullName:"Cecilia Cristea",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYQ65QAG/Profile_Picture_1621007741527",institutionString:null,institution:{name:"Iuliu Hațieganu University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"40735",title:"Dr.",name:"Gil",middleName:"Alberto Batista",surname:"Gonçalves",slug:"gil-goncalves",fullName:"Gil Gonçalves",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYRLGQA4/Profile_Picture_1628492612759",institutionString:null,institution:{name:"University of Aveiro",institutionURL:null,country:{name:"Portugal"}}},{id:"211725",title:"Associate Prof.",name:"Johann F.",middleName:null,surname:"Osma",slug:"johann-f.-osma",fullName:"Johann F. 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