Mean values of selected soil properties under three different tree species.
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Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
\n\nThis achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
\n\nWe are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
\n\nThank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
\n\n\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"5486",leadTitle:null,fullTitle:"Quality Control and Assurance - An Ancient Greek Term Re-Mastered",title:"Quality Control and Assurance",subtitle:"An Ancient Greek Term Re-Mastered",reviewType:"peer-reviewed",abstract:"Quality control and assurance cover a diverse area of modern life and play, undeniably, an important role. This book brings together a collection of international papers that showcase examples of current research and practice in industry and the medical profession. It is hoped that engineers, researchers and scientists will be assisted in their continuous quest for excelling in qualitative aspects. The Ancient Greek word arete means excellence or virtue and defines the highest qualitative state: a man’s effectiveness and skill in goodness (optimum potentiae). Indeed, Ancient Greeks believed that without quality control, specifications are useless and may result to illegitimacy, which in turn may become a threat to society itself.",isbn:"978-953-51-2922-6",printIsbn:"978-953-51-2921-9",pdfIsbn:"978-953-51-6696-2",doi:"10.5772/63135",price:119,priceEur:129,priceUsd:155,slug:"quality-control-and-assurance-an-ancient-greek-term-re-mastered",numberOfPages:228,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"549fefebffcb2f610fb669f6eb86c785",bookSignature:"Leo D. Kounis",publishedDate:"February 22nd 2017",coverURL:"https://cdn.intechopen.com/books/images_new/5486.jpg",numberOfDownloads:19342,numberOfWosCitations:16,numberOfCrossrefCitations:14,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:26,numberOfDimensionsCitationsByBook:0,hasAltmetrics:1,numberOfTotalCitations:56,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 26th 2016",dateEndSecondStepPublish:"May 17th 2016",dateEndThirdStepPublish:"August 21st 2016",dateEndFourthStepPublish:"November 19th 2016",dateEndFifthStepPublish:"December 19th 2016",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"111582",title:"Dr.",name:"Leo",middleName:"Dimitrios",surname:"Kounis",slug:"leo-kounis",fullName:"Leo Kounis",profilePictureURL:"https://mts.intechopen.com/storage/users/111582/images/system/111582.jpg",biography:"Leo D. Kounis is the Head of the Department of Communication and Informatics Battalion at the Hellenic Ministry of Defense, Hellenic National Defense General Staff. He obtained his BEng (Hons) degree in Manufacturing Systems Engineering, his MSc in Quality Engineering, and his PhD in Systems Reliability from the University of Hertfordshire, UK. Dr. Kounis has worked as a senior quality engineer in a number of private companies in Greece, and has acted as a part-time lecturer and scientific advisor in academia. His research interests are focused in the area of quality, transportation, and sustainable energy. He has published a number of scientific papers.",institutionString:"Hellenic National Defense General Staff",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"3",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"730",title:"Quality Control Management",slug:"engineering-control-engineering-quality-control-management"}],chapters:[{id:"53946",title:"The Evolution of Quality Concepts and the Related Quality Management",doi:"10.5772/67211",slug:"the-evolution-of-quality-concepts-and-the-related-quality-management",totalDownloads:4401,totalCrossrefCites:5,totalDimensionsCites:6,hasAltmetrics:1,abstract:"Enterprises usually adopt some quality practices to control the product quality during the manufacturing process in order to assure the delivery of qualitative good products to customers. The quality practices or quality management systems adopted by industries will further evolve due to the changes of quality concepts as time goes by. This chapter discusses the change of quality concepts and the related revolution of quality management systems in the past century. The quality concepts were gradually changed from the achievement of quality standards, satisfaction of customer needs, and expectations to customer delight. Since merely satisfying customers is not enough to ensure customer loyalty, the enterprises gradually focus on customers’ emotional responses and their delight in order to pursue their loyalty. The emotion of “delight” is composed of “joy” and “surprise,” which can be achieved as the customers’ latent requirements are satisfied. Thus, the concept of “customer delight” and the means to provide the innovative quality so as to meet the unsatisfied customers’ latent needs are elaborated on. Finally, a framework of innovation creation is developed that is based on the mining of customer's latent requirements. This outline will manifest the essential elements of the related operation steps.",signatures:"Ching-Chow Yang",downloadPdfUrl:"/chapter/pdf-download/53946",previewPdfUrl:"/chapter/pdf-preview/53946",authors:[{id:"11862",title:"Prof.",name:"Ching-Chow",surname:"Yang",slug:"ching-chow-yang",fullName:"Ching-Chow Yang"}],corrections:null},{id:"53024",title:"Key Aspects for Implementing ISO/IEC 17025 Quality Management Systems at Materials Science Laboratories",doi:"10.5772/66100",slug:"key-aspects-for-implementing-iso-iec-17025-quality-management-systems-at-materials-science-laborator",totalDownloads:2804,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Implementing a quality management system based on the requirements specified in ISO/IEC 17025 standard at materials science laboratories is challenging, mainly due to two main factors: (i) the high technical complexity degree of some tests used for materials characterization and (ii) the fact that most materials science laboratories provide materials characterization tests and also carry out research and development activities. In this context, this chapter presents key subjects while implementing a quality management system at materials science laboratories and some considerations on strategies for effectively implementing such systems.",signatures:"Rodrigo S. Neves, Daniel P. Da Silva, Carlos E. C. Galhardo, Erlon H.\nM. Ferreira, Rafael M. Trommer and Jailton C. Damasceno",downloadPdfUrl:"/chapter/pdf-download/53024",previewPdfUrl:"/chapter/pdf-preview/53024",authors:[{id:"20571",title:"Prof.",name:"Erlon H.",surname:"Martins Ferreira",slug:"erlon-h.-martins-ferreira",fullName:"Erlon H. Martins Ferreira"},{id:"145815",title:"Dr.",name:"Rodrigo",surname:"De Santis Neves",slug:"rodrigo-de-santis-neves",fullName:"Rodrigo De Santis Neves"},{id:"145816",title:"Dr.",name:"Carlos",surname:"Eduardo Cardoso Galhardo",slug:"carlos-eduardo-cardoso-galhardo",fullName:"Carlos Eduardo Cardoso Galhardo"},{id:"159056",title:"Dr.",name:"Jailton",surname:"Damasceno",slug:"jailton-damasceno",fullName:"Jailton Damasceno"},{id:"191863",title:"Dr.",name:"Daniel",surname:"Fernandes",slug:"daniel-fernandes",fullName:"Daniel Fernandes"},{id:"191865",title:"Dr.",name:"Rafael",surname:"Mello Trommer",slug:"rafael-mello-trommer",fullName:"Rafael Mello Trommer"}],corrections:null},{id:"53144",title:"Youden Two-Sample Method",doi:"10.5772/66397",slug:"youden-two-sample-method",totalDownloads:2398,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The results obtained when testing materials, equipment and procedures are not generally identical. Factors that influence the magnitude of the results are not fully controllable. As such, the interpretation and analysis of results must take into account the variations caused by numerous and random unavoidable causes. Intercomparison exercises are considered of being of importance, as they do allow the examination of the analytical process and their generated results. Youden plot is particularly aimed at interlaboratory comparisons. The raw results provided by the participating laboratories are treated by a statistical method applied by the centre performing the trial. In order to materialize this, two similar materials with small differences in the concentration of the characteristics are required. The advantage of Youden analysis is its ability to separate the random errors with a minimum effort by participants in the design from the point of view of the analytical requirement. This book chapter illustrates the method that has been applied to elaborate on data covering a diverse scientific field: polyunsaturated fatty acids in fat and oils, total blood cholesterol and aspirin in pharmaceutical preparations. Finally, liquid chromatography with tandem mass spectrometry detector has been applied to the determination of an emerging contaminant, methylparaben (MeP), in surface waters.",signatures:"Julia Martín, Nieves Velázquez and Agustin G. Asuero",downloadPdfUrl:"/chapter/pdf-download/53144",previewPdfUrl:"/chapter/pdf-preview/53144",authors:[{id:"190870",title:"Dr.",name:"Agustín G.",surname:"Asuero",slug:"agustin-g.-asuero",fullName:"Agustín G. Asuero"},{id:"190871",title:"Dr.",name:"Julia",surname:"Martín",slug:"julia-martin",fullName:"Julia Martín"},{id:"195055",title:"Dr.",name:"Nieves María",surname:"Velázquez",slug:"nieves-maria-velazquez",fullName:"Nieves María Velázquez"}],corrections:null},{id:"52915",title:"Using Lot Quality Assurance Sampling to Monitor the Prevalence of Abortions and the Quality of Reproductive Health Care in Armenia",doi:"10.5772/66092",slug:"using-lot-quality-assurance-sampling-to-monitor-the-prevalence-of-abortions-and-the-quality-of-repro",totalDownloads:1570,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Monitoring abortion prevalence is essential to plan control efforts. Lot Quality Assurance Sampling (LQAS) is an inexpensive, reliable method for monitoring abortion prevalence and access to quality reproductive health (RH) services. This chapter presents survey results from 2000 in three sites of Armenia (Gyumri, Gavar and Goris) using LQAS principles (i.e., 44%, 95% CI: ±6% of women had an induced abortion in their lifetime, a total abortion rate (TAR) of 2.0 abortions per woman). Modern contraceptive use was lowest in Goris (16%. 95% CI: ±7%) and highest in Gyumri (43%, 95% CI: ±11%). Only 37% (95% CI: ±9%) of women with an induced abortion received family planning information and 21% (95% CI: ±4%) of mothers were counselled about family planning after delivery. While limited access to family planning information and contraceptives is still an issue in Armenia, recently new reproductive health priorities—such as infertility, sex-selective abortions and abortions due to socio-economic difficulties—have become more common and can be investigated using LQAS in both community surveys and health facility assessments. This study demonstrates that measuring national abortion prevalence and access to services mask underlying variations; the awareness of which is essential for health program planning.",signatures:"Joseph J. Valadez and Lusine Mirzoyan",downloadPdfUrl:"/chapter/pdf-download/52915",previewPdfUrl:"/chapter/pdf-preview/52915",authors:[{id:"191876",title:"Prof.",name:"Joseph",surname:"Valadez",slug:"joseph-valadez",fullName:"Joseph Valadez"},{id:"195035",title:"Dr.",name:"Lusine",surname:"Mirzoyan",slug:"lusine-mirzoyan",fullName:"Lusine Mirzoyan"}],corrections:null},{id:"53019",title:"A Framework to Manage Quality of Enterprise Content Management Systems",doi:"10.5772/66199",slug:"a-framework-to-manage-quality-of-enterprise-content-management-systems",totalDownloads:1387,totalCrossrefCites:0,totalDimensionsCites:3,hasAltmetrics:1,abstract:"There is a wide range of enterprise content management (ECM) systems which supports, among other things, document management processes, records management and Web content management. However, each of these systems has many features and some of them can meet organizational needs depending on the scale, sector and workflow of the organization. In addition, it is very common that organizations are unaware of what ECM system best fits their needs, since each company has its particular scope and strategic objectives. This chapter is contextualized within the real project called THOT designed for the Andalusian Public Administration in Spain. The aim of this project is to study in detail ECM systems and propose an objective method to compare them for the specific scope and strategic objective of organizations. Quality evaluation framework (QuEF) has been adapted for this purpose.",signatures:"José González Enríquez, Francisco José Domínguez Mayo, Julián\nAlberto García García, María José Escalona Cuaresma and Manuel\nMejías Risoto",downloadPdfUrl:"/chapter/pdf-download/53019",previewPdfUrl:"/chapter/pdf-preview/53019",authors:[{id:"104846",title:"Dr.",name:"Maria",surname:"Escalona",slug:"maria-escalona",fullName:"Maria Escalona"},{id:"191332",title:"Ph.D.",name:"José",surname:"González",slug:"jose-gonzalez",fullName:"José González"},{id:"191493",title:"Dr.",name:"F.J.",surname:"Domínguez-Mayo",slug:"f.j.-dominguez-mayo",fullName:"F.J. Domínguez-Mayo"},{id:"191494",title:"Dr.",name:"J.A.",surname:"García-García",slug:"j.a.-garcia-garcia",fullName:"J.A. García-García"},{id:"195075",title:"Dr.",name:"M.",surname:"Mejías",slug:"m.-mejias",fullName:"M. Mejías"}],corrections:null},{id:"53267",title:"Exploring the Relationship of Supply Chain Risk Management to Quality Management",doi:"10.5772/65847",slug:"exploring-the-relationship-of-supply-chain-risk-management-to-quality-management",totalDownloads:1524,totalCrossrefCites:4,totalDimensionsCites:4,hasAltmetrics:0,abstract:"This research explores the relationship between an organization's supply chain risk management (SCRM) maturity and quality maturity. SCRM maturity was measured using a survey questionnaire sent to organizations in the USA. Quality maturity was assessed via ISO 9001:2008 certification status as well as through a survey questionnaire of total quality management (TQM) practices for organizations in the USA. The results suggest that ISO 9001:2008 is not related to SCRM maturity, while TQM maturity is related to SCRM maturity. Organizations with more mature TQM programs appear to also have more mature SCRM programs.",signatures:"Tyler Florio",downloadPdfUrl:"/chapter/pdf-download/53267",previewPdfUrl:"/chapter/pdf-preview/53267",authors:[{id:"195084",title:"Mr.",name:"Tyler",surname:"Florio",slug:"tyler-florio",fullName:"Tyler Florio"}],corrections:null},{id:"52958",title:"ALAMEDA Ecosystem: Centering Efforts in Software Testing Development",doi:"10.5772/66043",slug:"alameda-ecosystem-centering-efforts-in-software-testing-development",totalDownloads:2074,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"One of the most important and critical aspects to improve the quality assurance in software is to improve the testing process by utilizing techniques and tools, which will enhance the software testing process, making it more effective and efficient. This chapter presents ALAMEDA ecosystem, a software package that centers its efforts in software testing development and is a result from a real-world project. ALAMEDA provides support to lifecycles focused on the generation, implementation, and testing organization from the earliest stages of software development. In addition, the ecosystem provides an environment of rating the degree of compliance of organizations with the International Standard for Testing ISO/IEC-29119. It is proposed as a tool to use during the various iterations that may occur in an agile software development process.",signatures:"José González Enríquez, Julián Alberto García-García, Francisco José\nDomínguez-Mayo and María José Escalona Cuaresma",downloadPdfUrl:"/chapter/pdf-download/52958",previewPdfUrl:"/chapter/pdf-preview/52958",authors:[{id:"104846",title:"Dr.",name:"Maria",surname:"Escalona",slug:"maria-escalona",fullName:"Maria Escalona"},{id:"191332",title:"Ph.D.",name:"José",surname:"González",slug:"jose-gonzalez",fullName:"José González"},{id:"191493",title:"Dr.",name:"F.J.",surname:"Domínguez-Mayo",slug:"f.j.-dominguez-mayo",fullName:"F.J. Domínguez-Mayo"},{id:"191494",title:"Dr.",name:"J.A.",surname:"García-García",slug:"j.a.-garcia-garcia",fullName:"J.A. García-García"}],corrections:null},{id:"53099",title:"Improving Quality Assurance in Multidisciplinary Engineering Environments with Semantic Technologies",doi:"10.5772/66222",slug:"improving-quality-assurance-in-multidisciplinary-engineering-environments-with-semantic-technologies",totalDownloads:1556,totalCrossrefCites:4,totalDimensionsCites:8,hasAltmetrics:0,abstract:"In multidisciplinary engineering (MDE) projects, for example, automation systems or manufacturing systems, stakeholders from various disciplines, for example, electrics, mechanics and software, have to collaborate. In industry practice, engineers apply individual and highly specialized tools with strong limitation regarding defect detection in early engineering phases. Experts typically execute reviews with limited tool support which make engineering projects defective and risky. Semantic Web Technologies (SWTs) can help to bridge the gap between heterogeneous sources as foundation for efficient and effective defect detection. Main questions focus on (a) how to bridge gaps between loosely coupled tools and incompatible data models and (b) how SWTs can help to support efficient and effective defect detection in context of engineering process improvement. This chapter describes success-critical requirements for defect detection in MDE and shows how SWTs can provide the foundation for early and efficient defect detection with an adapted review approach. The proposed defect detection framework (DDF) suggests different levels of SWT contributions as a roadmap for engineering process improvement. Two selected industry-related real-life cases show different levels of SWT involvement. Although SWTs have been successfully applied in real-life use cases, SWT applications can be risky if applied without good understanding of success factors and limitations.",signatures:"Dietmar Winkler, Marta Sabou and Stefan Biffl",downloadPdfUrl:"/chapter/pdf-download/53099",previewPdfUrl:"/chapter/pdf-preview/53099",authors:[{id:"95798",title:"Dr.",name:"Dietmar",surname:"Winkler",slug:"dietmar-winkler",fullName:"Dietmar Winkler"},{id:"135962",title:"Prof.",name:"Stefan",surname:"Biffl",slug:"stefan-biffl",fullName:"Stefan Biffl"},{id:"196030",title:"Dr.",name:"Marta",surname:"Sabou",slug:"marta-sabou",fullName:"Marta Sabou"}],corrections:null},{id:"53522",title:"The Use of Control Charts in the Study of Bitcoin’s Price Variability",doi:"10.5772/66360",slug:"the-use-of-control-charts-in-the-study-of-bitcoin-s-price-variability",totalDownloads:1638,totalCrossrefCites:0,totalDimensionsCites:2,hasAltmetrics:0,abstract:"The focus of this research is bitcoin’s variability and its comparison with the variability of the EURO/USD exchange rate. Virtual currencies have been evolving in a dynamic way in the last few years. Under 600 different virtual currencies, the most successful was bitcoin. Its adherents saw in it an alternative to the traditional means of payments allowing the performance of real-time transactions at low costs. The accessibility, where no financial infrastructure is ensured or where either limited or no international agreements exist between financial and banking institutions was also an advantage. The opponents perceived this as a temporary curiosity with no future. Time confirmed that bitcoin has gained on popularity and the exchange rate to the main currencies rose in a dynamic way. The analysts, however, underline that the bitcoin is too volatile and unpredictable, so it cannot compete against the main currencies. The aim of this research is to compare the bitcoin (BTC) to US Dollar (USD) exchange rate and Euro to USD exchange rate volatility using control charts. The results have shown that BTC/USD exchange rate volatility is strongly affected by unexpected price jumps during the period (2010–2016), an act that significantly distinguishes it from more stable and predictable EUR/USD exchange rate variability.",signatures:"Beata Szetela",downloadPdfUrl:"/chapter/pdf-download/53522",previewPdfUrl:"/chapter/pdf-preview/53522",authors:[{id:"190806",title:"Ph.D. Student",name:"Beata",surname:"Szetela",slug:"beata-szetela",fullName:"Beata Szetela"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"6235",title:"Quality Management Systems",subtitle:"a Selective Presentation of Case-studies Showcasing Its Evolution",isOpenForSubmission:!1,hash:"a7bb324cdb11307c932cb6a7966a1e00",slug:"quality-management-systems-a-selective-presentation-of-case-studies-showcasing-its-evolution",bookSignature:"Leo D. 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\r\n\tSchiff base (imine -N=CH-) is one of a popular group of organic compounds prepared from primary amines and aldehyde. Not only as organic compounds but also as ligands for metal complexes, a number of studies have been carried out so far. In this context, this book aims to record a wider range of interdisciplinary content on Schiff base compounds, with an emphasis on the latest advances. This book will aim to compile research results, commentary, reviews, etc., that have dealt with preparation, spectroscopy, crystallography, (asymmetric) synthetic roles, physical properties (magnets, optics, and so on), computational chemistry, and/or theoretical chemistry and their discussions. The book will also intend to focus on Schiff base and its strong connection from organic chemistry to biochemistry or polymer materials chemistry.
",isbn:"978-1-80355-679-6",printIsbn:"978-1-80355-678-9",pdfIsbn:"978-1-80355-680-2",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"ce51efbe2cae97ca3199350ef6c498ec",bookSignature:"Dr. Takashiro Akitsu",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/12078.jpg",keywords:"Schiff Base, Imine, Azomethine, Synthesis, Characterization, Crystal Structure, Chirality, Liquid Crystals, Polymers or Biopolymers, Metal Complex, Salen-Type Ligand, Computational Chemistry",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 20th 2022",dateEndSecondStepPublish:"June 17th 2022",dateEndThirdStepPublish:"August 16th 2022",dateEndFourthStepPublish:"November 4th 2022",dateEndFifthStepPublish:"January 3rd 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"21 days",secondStepPassed:!1,areRegistrationsClosed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"A professor from the University of Science, Japan, has published 220 articles and book chapters. Dr. Akitsu studied at the Institute for Protein Research (metalloproteins), Keio University (photo and magnetic functional organic/inorganic hybrid compounds), and Stanford University (physical and bioinorganic chemistry) before moving to Tokyo University of Science.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"147861",title:"Dr.",name:"Takashiro",middleName:null,surname:"Akitsu",slug:"takashiro-akitsu",fullName:"Takashiro Akitsu",profilePictureURL:"https://mts.intechopen.com/storage/users/147861/images/system/147861.jpg",biography:"Takashiro Akitsu, Ph.D., is now a professor in the Department of Chemistry, Faculty of Science Division II, Tokyo University of Science, Japan. Studying crystal and electronic structures of chiral copper complexes, he graduated from Osaka University and obtained his Ph.D. in Physical and Inorganic Chemistry in 2000. Dr. Akitsu studied at the Institute for Protein Research (metalloproteins), Keio University (photo and magnetic functional organic/inorganic hybrid compounds), and Stanford University (physical and bioinorganic chemistry) before moving to Tokyo University of Science. He has published 220 articles and book chapters. 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It is associated with important complications that may reduce life expectancy of these patients [2, 3].
\nMost acromegalic patients die from cardiovascular, cerebrovascular, or respiratory diseases [3, 4]. Nevertheless, in the past two decades, some studies have also described an association between acromegaly and an increased risk of some cancers such as colorectal and thyroid cancer (TC), which is the most common endocrine malignancy, among others [5].
\nPart of the difficulty in determining the true incidence of cancer in this population is due to the relative rarity of acromegaly [6]. On the other hand, with improvement in surgical and radiotherapeutic procedures as well as advances in medical treatment, an increase of the survival rate of patients with acromegaly has been shown. As a result, patients may have a longer exposure to high GH levels [7].
\nAs the prevalence of thyroid cancer has been shown to increase among patients with acromegaly, this should draw attention for clinicians to investigate thyroid disease, particularly thyroid cancer.
\nThe association between acromegaly and TC is supported by preclinical data showing that GH-IGF system plays an important role in cancer development and progression [6]. However, clinical studies that addressed the association between acromegaly and cancer produced controversial results, partly due to the different methodological approaches used (case-control and population-based designs) [8].
\nA comprehensive meta-analysis showed an increased risk of both nodular thyroid disease (NTD) (OR = 6.9, RR = 2.1) and TC (OR = 7.5, RR = 7.2) in acromegaly. It showed a prevalence slightly below 60% of NTD and of around 4% of TC [8]. Within this context, a consistent Brazilian multicentric study with 124 acromegalic patients in a case-control design showed a higher prevalence of 7.2% for TC and 0.7% in the control group [9].
\nThese findings may result from the fact of improving diagnostic and treatment of acromegaly extending the life duration which increases the prevalence of both benign and malignant neoplasms [3, 4, 5, 6, 7, 8, 9, 10, 11].
\nOn the other hand, the co-occurrence of autoimmune thyroid diseases and acromegaly is not common. So far only a handful of cases of Graves-Basedow disease in acromegalic patients have been reported, while Hashimoto’s disease occurs more frequently (4.6%) [12, 13].
\nThe pituitary gland integrates hormonal signs that control several homeostatic processes such as metabolism, growth, and reproduction. Cell clusters localized in the anterior pituitary, somatotrophs, secrete GH responsible for cellular proliferation through membrane-bound growth hormone receptor (GHR) present in various organs and systems [14]. The interaction between GH and GHR results in activation of intracellular protein Janus kinase 2 (JAK2). As shown in Figure 1, once phosphorylated JAK2 activates the signal transducers and activators of transcription (STAT) protein that is translocated to the nucleus and initiates transcription of genes in response to GH [15], the STAT is able to bind to IGF-I promoter regulating the transcription of this gene [16]. Thus, the presence of GH can induce the synthesis of IGF-I that occurs mainly in the liver and is composed of 70 amino acids and has mitotic and anti-apoptotic effects [1].
\nActivation of JAK/STAT pathway mediated by GH (growth hormone). (a) JAK/STAT pathway components are inactive. (b) GH leads to dimerization of its receptor promoting phosphorylation of JAK and consequent activation of STAT proteins. (c) Once activated, STAT forms dimers that are translocated to the cell nucleus. (d) The STAT dimers in the nucleus are capable of binding to
In the vast majority of cases, the excess of GH in acromegaly is originated from proliferating somatotrophs (somatotropinoma). The pituitary adenomas are of monoclonal origin, indicating that the tumor rises from a single cell that acquires proliferative advantage [17]. The primary defect that leads to development of somatotropinoma may result from genetic and epigenetic alterations inducing the activation of oncogenes or inactivation of tumor suppressor genes [1]. Mutations in the alpha subunit of transmembrane G protein is observed in 40% of GH-secreting tumors [1]. This abnormality may cause constitutive activation of cyclin AMP (cAMP) and consequent hypersecretion of GH. Loss of expression of proapoptotic molecules such as GADD45γ (growth arrest and DNA damage-inducible 45γ protein) and overexpression of oncoproteins, including PTTG (pituitary tumor-transforming gene), are phenomena also observed in pituitary adenomas [17, 18].
\nMost cases of acromegaly occur sporadically; however, approximately 5% of cases may be related to inherited diseases such as multiple endocrine neoplasia type 1 (MEN1), Carney complex (CNC), and familial isolated pituitary adenoma (FIPA) [17]. Germline mutations in aryl hydrocarbon receptor-interacting protein (
The serum GH excess may promote proliferation and suppress apoptosis in many tissues [15]. Thus, it is suggested that acromegaly is responsible for the increased risk for development of many malignancies. PTC is the most common thyroid cancer observed in acromegaly [7, 9]. This type of pituitary tumor can also be associated with benign thyroid conditions such as diffuse and nodular goiters [9].
\nThe mechanism of thyroid carcinogenesis in acromegaly is attributed to an autocrine/paracrine loop for GH/IGF-I in tumor tissue [8]. As the thyroid follicular cells also produce IGF-I and express genes encoding IGF-IR, the long-term exposure of thyrocyte to high GH/IGF-I levels may work synergically with this loop in promoting goiter development and malignant transformation [20].
\nAs shown in Figure 2, the molecular oncogenesis of PTC is mainly related to deregulation of mitogen-activated protein kinase (MAPK) signaling pathway and involves point mutations in
MAPK and PI3K pathways. (a) Growth factors bind to receptor tyrosine kinase and trigger the activation of (b) MAPK and/or (c) PI3K-AKT. (d) The signaling mediated to both pathways promotes the transcription of gene associated to different cellular processes such as proliferation and survival.
BRAF (B-type RAF kinase) is a serine threonine kinase considered the most potent MAPK activator. This protein regulates important cellular processes such as proliferation, differentiation, and apoptosis [1].
\nIn PTC, the main mechanism for activation of
In acromegalic patients, the importance of
The
Point mutations are commonly restricted at codon 61 of the
The
Besides the potential classic marker, other molecules have been evaluated in relation to their implication on PTC development in acromegaly, among them are IGF-I, IGF-IRβ, AIP, AHR, and galectin-3 (Gal-3) [20, 23, 24, 27].
\nThe analysis of immunohistochemical staining for IGF-IRβ revealed a high expression of this receptor in PTC samples [20]. Although differences in IGF-IRβ tumoral staining between PTC patients with and without acromegaly have not been observed, this marker had significantly less expression in adjacent normal tissue of patients with acromegaly. These data suggest that high GH levels may trigger autocrine and paracrine effects of IGF-I in thyroid follicular cells resulting in overexpression of IGF-IRβ in tumor tissue of acromegalic patients. In line with these results, it was observed that PTC patients with acromegaly have higher expression of IGF-I than PTC cases without acromegaly [27]. Additionally, an intense expression was verified of Gal-3 in PTC with acromegaly, speculating a possible influence of this protein on thyroid carcinogenesis.
\nAs previously mentioned, inactivation of
NTD seems to be significantly more frequent in patients with acromegaly. Even palpable thyroid nodules occur significantly more often in these patients [9, 13].
\nPeriodic thyroid ultrasound (US) and careful evaluation of detected lesions are important parts in the follow-up of acromegalic patients. The sonographic characteristics considered to be suspicious of TC, such as microcalcifications, irregular margins (infiltrative and microlobulated), taller than wide shape, and rim calcifications with small extrusive soft tissue component (evidence of extrathyroidal extension), are the same of the general population with NTD [5, 9].
\nFine-needle aspiration (FNA) is the procedure of choice in the evaluation on NTD, and it should be performed when clinically indicated according to nodule’s size and US appearance. The FNA cytology result must be reported using the Bethesda System for Reporting Thyroid Cytopathology [9, 28].
\nIn summary, as the risk of malignancy in thyroid nodules in these patients is about 8%, which is in the range considered for the general population, the management of NTD should follow the current guidelines [9, 28].
\nAlthough there is a risk of TC in acromegalic patients, its clinical behavior does not seem to be different [5]. Therefore, acromegalic patients with TC may be treated with total thyroidectomy or hemithyroidectomy according to its FNA result and size and the presence of clinically apparent metastatic lymph nodes [28].
\nBefore surgery, we suggest that all acromegalic patients should do a preoperative voice assessment (preoperative laryngeal exam—laryngoscopy) because they frequently have soft tissue thickening and edema of the tongue, pharynx, and upper airways [3]. Also, they must have a careful evaluation of comorbidities as hypertension, diabetes mellitus, and cardiovascular disease [3].
\nAfter surgery, these patients may or may not receive radioiodine depending, if it is a differentiated TC, on its risk of recurrence [28]. Studies about the relationship between medullary thyroid cancer (MTC) and acromegaly are lacking.
\nThe frequency of US and laboratory tests during TC follow-up should follow the current guidelines.
\nNTC and TC are more frequent in acromegalic patients. On the other hand, the studies about potential mechanisms involved in this association between TC and acromegaly are still scarce, and besides they include small sample sizes. Furthermore, in these few reports, there is no marker clearly implicated on diagnosis or prognosis of PTC. Thus, further studies with this approach are needed.
\nWe suggest that acromegalic patients should be routinely screened by thyroid ultrasound and during their follow-up as necessary. Its management should follow the current guidelines. This is very important because it may allow early diagnosis and treatment of TC.
\nWe would like to thank Doctor Gilvan Nascimento Cortês for the review of the manuscript.
\nThere is no conflict of interest.
Forest ecosystems are a fundamental part of the biosphere as they govern global primary production and biogeochemical cycling [1]. The dynamics of decomposition and nutrient cycling is driven by soil microbial communities [2] and their enzymatic potential [3]. Assessments of soil microbial community composition are often coupled with the assessment of soil processes (C storage and nutrient cycling) through different enzymatic activities to better address the ecosystem function [4]. Some of the principal enzyme activities assayed in soils are involved in carbon cycling (β-glucosidase, α-galactosidase) with potential importance for C sequestration [5]. Other enzymes such as β-glucosaminidase are important in the decomposition of more complex compounds such as chitin [6, 7] Phosphorus cycling enzymes (acid and alkaline phosphatases and phosphodiesterase) are important since most soils are P deficient [8] and microbial enzyme activity (EA) plays a vital role in the availability of P in soils.
\nBoth microbial communities and enzyme activities are sensitive to biotic and abiotic disturbances. For example, low soil moisture may result in low enzyme activity [9] and rainfall pulses may result in pulses of microbial growth which may lead to pulsed secretion of soil enzymes promoting a temporary increase of soil enzyme activity [10].
\nUnderstanding the dynamics of forest soil ecosystem depends on elucidating the contribution of individual plant species to the soil biota and the process that they regulate [11]. Determining and quantifying plant species effects under natural conditions can be difficult due to environmental noise and the interaction among species present in an area [12]. Little is known about the effects of dominant vegetation on the soil biogeochemical processes such as enzyme activities [13]. Even less is known about the plant-soil-microbial interactions that take place in extreme environments such as tropical dry forest.
\nNearly 42% of tropical forests around the world are seasonally dry plant communities, where around half of the Central American and Caribbean land area is characterized by a tropical or subtropical dry forest climate. In the Coastal Plateau of the Guánica Dry Forest (GDF), an UNESCO/MAB Biosphere Reserve, dwarfed trees grow isolated from one another in the cracks of the calcareous platform, forming individual islands of fertility [14]. A lack of interspecific competition is observed, as tolerance to environmental stress and scarcity of space for establishment make it difficult for aboveground and belowground competition, also contributing to the evenness of tree species found in this area [14]. The substrate is derived from limestone made from marine deposits that vary throughout the forest from deep alluvial fans to exposed fractured limestone with shallow soil pockets [15]. This naturally occurring plant community provides the ideal conditions to determine how specific tree species affect the soil microbial community composition and enzymatic potential in a dry forest.
\nIn order to understand how trees impact soil microbial communities, a five-month study was conducted at the GDF. We selected three dwarfed, isolated tree species (a pantropical species and two native species) that are highly distributed among the forest, hypothesizing that these trees may harbour different microbial community structure and activities. The tree species selected complied with the following requirements: (1) that trees were growing in cracks isolated from other trees by exposed rock and (2) that their litter and belowground substrate originated from their own residue decomposition [16]. Additionally, this forest experiences bimodal and pulsed precipitation patterns [17, 18] that may contribute in the alteration of the microbial dynamics and nutrient turnover of the forest. Our objectives were: (1) to determine if tree species traits had an effect on the soil microbial community structure and activities and (2) to determine the effects of sampling period on the soil microbial community structure and activities.
\nThe study was located in the coastal association of the Guánica Dry Forest latitude 66°53′30″W longitude 17°58′0″N [16, 17, 18]. In this area of the forest, dwarf individual adult trees from different species are separated by exposed rocks which prevent overlap among trees forming “monospecific tree islands” (\nFigure 1A\n and \nB\n). These “monospecific tree islands” have little or no mineral soil (\nFigure 1A\n), with a highly organic substrate composed of shallow monospecific litter and humus (\nFigure 1C\n and \nD\n), which varies according to variations in the ground relief and season from 2 to 8 cm depth [16]. These characteristics make this forest an ideal system to study tree species effects as there are no confounding effects of overlapping roots from other species. We chose three trees from three species, previously tagged and studied, that grow from 100 m to approximately 300 m from the coast.
\nDetailed description of study site. (A) Tree species that has established its growth in the cracks and crevices of the calcareous rock. Black arrow demonstrates a new soil pocket that is being formed as a by-product of the decomposition of the tree’s litter. (B and C)
The three tree species selected are:
Total organic matter, soil texture, pH and moisture analyses were completed. Soil total organic matter was determined by the ignition method. Soil texture was determined by the pipette method [19]. Soil pH was determined in a 1:5 (soil:water) mixture using an Orion 420A pH Meter [20]. Soil moisture was determined by weighing and drying 1 g of soil at 105°C during 24 h [20].
\nMicrobial community structure was evaluated by the ester-linked (EL)-FAME method as described by [21]. A total of 3 g of field moist soil was used for each sample, and four steps were completed: (a) saponification and methylation of ester-linked fatty acids were performed by incubating the 3 g of soil in 15 ml of 0.2 M KOH in methanol at 37°C during 1 h—samples were vortexed every 10 min during the incubation period and, after the incubation period was completed, 3 ml of 1.0 acetic acid was added to neutralize the mixture’s pH; (b) partitioning of the FAMEs into an organic phase was achieved by adding 10 ml of hexane and centrifuging the preparations at 480×
Study of enzyme activities was performed as described in [4, 6]. The activities of enzymes relevant in C cycling (β-glucosidase), C and N cycling (β-glucosaminidase), P cycling (alkaline phosphatase, acid phosphatase, phosphodiesterase) and in the S cycle (arylsulphatase) were assayed using 0.5 g of air-dried soil (<2 mm). Duplicate replicates and one control were used for all the soils that were tested; furthermore, the appropriate substrate was used for each assay and reactions were incubated for 1 h at 37°C at their optimal pH as described in [4]. For the controls, the substrate was added after the 1-h incubation period and subtracted from a sample control value. Enzyme activity is expressed in mg p-nitrophenol (PN) released in kg−1 soil h−1.
\nAll data analysed with JMP software were checked for normality and transformed to log10. To determine differences in soil FAMEs, enzyme activity and selected soil properties due to tree species and sampling periods, canonical discriminate analysis (CDA) was conducted with the JMP program. The first and second canonical discriminate functions were utilized to determine the distribution of enzyme activity and FAMEs as influenced by each tree species and by sampling period. SigmaPlot 10 software was used to conduct two-way analysis of variance (2-way ANOVA). Two-way analysis of variance was used to establish the effect of plant species and sampling period on soil community structure, enzyme activities and selected soil properties.
\nSoil samples under all tree species were predominantly organic (65–85%). The mineral part of the soil is silty loam to silty clay loam (20–36% clay, 0.4–0.7% sand, 63–80% silt) depending on the tree location in the study area (data not shown). Soil pH was neutral to alkaline for all tree species (\nTable 1\n). Two-way ANOVA did not demonstrate an interaction between tree species and sampling months (\nTable 2\n). Available N varied significantly with regard to monthly sampling and not to tree species (\nTable 2\n). Available Ca and P varied significantly with regard to tree species (\nTable 2\n). Total N and C:N ratio varied significantly with regard to tree species.
\nMean values of selected soil properties under three different tree species.
Two-way analysis of variance for the effects of species (
Six enzyme activities (EAs) were assessed representing C (β-glucosidase), N (β-glucosaminidase), P (phosphodiesterase, alkaline and acid phosphatase) and S (arylsulphatase) cycling. There was a clear and significant separation in the soil enzyme activity between the tree species in this forest, while there was no distinct trend due to the sampling time (\nFigure 2\n). Most of the separation on the metabolic capacity of the soil according to these six EAs was observed along axis 1, which explained 87.22% of the variability.
Canonical discriminate analysis of the soil enzyme activity at the Guánica Dry Forest. (A) canonical discriminate analysis of the soil enzyme activity as affected by tree species (
Two-way analysis of variance was used to establish the effect of plant species and sampling period on soil enzyme activities. The only enzymes that were significantly affected by plant species were alkaline phosphatase (F = 8.18; P < 0.001), β-glucosidase (F = 8.86; P < 0.001) and β-glucosaminidase (F = 5.97; P = 0.007) (\nTable 2\n). Alkaline phosphatase was the only enzyme affected by monthly variations (F = 4.375; P = 0.007). Two-way ANOVA does not demonstrate significant interactions between tree species and monthly variations.
\nA total of 56 FAMEs were identified in the samples analysed and 13 FAMEs were used as indicators of different microbial groups as affected by tree species traits and sampling time (\nTable 2\n). According to CDA, significant differences (P < 0.0001) were detected in the FAME profiles of the soil microbial community structure as affected by tree species traits (\nFigure 3A\n). Canonical axis 1 explained 83% of the variation. The soil microbial communities under
Canonical discriminate analysis of the soil FAMEs at the Guánica Dry Forest. (A) canonical discriminate analysis of the soil FAME profiles as affected by tree species (
\n
In addition to the effects of tree species on FAME profiles of the microbial community structure, there were also significant monthly variations (p = 0.001) according to CDA along CA1, which explained 68.81% of the variation (\nFigure 3B\n). The CDA revealed that July, September and August clustered together along axis 1. August was separated from other months due to the predominance of
Two-way analysis of variance was used to establish the effect of plant species and sampling period on soil community structure. The Gram-positive EL-FAMEs that were significantly affected due to tree species were iso15:0, iso17:0, antesio17:0, 10Me16:0 and 10Me17:0. Gram-negative markers affected by tree species were 17:0 cy and 19:0 cy and fungal markers were 18:1ω9c, 16:1ω5c, 18:2 ω6c and 18:3 ω6c. The Gram-positive EL-FAMEs that were significantly affected due to monthly variations were iso15:0, antesio15:0 and 10Me16:0. None of the Gram-negative markers were affected by monthly variations. Fungal markers 16:1ω5c, 18:2 ω6c and 18:3 ω6c were significantly affected by monthly variations. Two-way ANOVA did not demonstrate significant interactions between tree species and monthly variations (\nTable 2\n).
\nThe evaluated tree species had the ability of modifying soil microbial communities. We found that the relative abundance of 8 out of 13 markers was higher under
Species traits such as leaf nutrients, leaf toughness and specific leaf area (SLA, cm2/g) are known to contribute to the rate of decomposition of organic matter, which may drive the microbial community under each tree species [22, 23, 24]. Previous studies have determined that
Differences in the relative abundance of fungal markers at the Guánica Dry Forest could also be due to the microclimate of each tree species. The amplitude of temperature fluctuations encountered in this forest varies among plant species, a factor that has been documented to affect arthropods in this system [16]. The canopies of both
As found in our study, microbial community structure was influenced by monthly wet/dry events (\nFigure 3B\n). Previous studies have described significant responses of the soil microbial communities to wet/dry events [25]. Our results point towards differential responses between sporadic and continuous rainfall events. The fungal markers showed lower abundance during July and higher abundance during October and November. Saprophytic fungal marker (18:3 ω6c, 18:2 ω6c) and the mycorrhiza marker 16:1 ω5c were more susceptible to monthly rainfall variations (p < 0.0001) than any other microbial group. As a consequence of rainfall pulses, adventitious roots were observed in the soil substrate, which serves as a surface area for the establishment of arbuscular mycorrhizal fungi. Although arbuscular mycorrhizal fungi enhance P absorption in root systems, we did not find any correlation between available P and mycorrhizal marker 16:1 ω5c.
\nAlthough fungal markers showed greater monthly fluctuations, the G+ bacterial marker
Our results show that enzyme activity is highly dependent on the soil microbial community structure of each tree species. Tree species was a strong modulator of soil enzyme activity when compared to monthly climatic variations. Although all enzymes tested were active under each tree species, activity of certain enzymes was higher under specific tree species. For instance, acid phosphatase, alkaline phosphatase and β-glucosidase activity was higher under
Tree species idiosyncratic traits affect not only the structure of microbial communities but also their enzyme activities. Alkaline phosphatase under
Microbial phosphatase activity is crucial for the supply of inorganic phosphate in this system. Soils at the Guánica Dry Forest are P limited due to the high amount of carbonates from the underlying calcareous substrate. In this study, phosphatases were the most active enzymes, microbial communities are allocating resources to balance the P deficiency of the Guánica soils. When P deficiency is present in a system, increased phosphatase activity occurs as a response to P starvation [29]. A mechanism that compensates soil P deficiency is the inoculation of the arbuscular mycorrhizae, which are known to enhance plant P availability via the production of phosphatase enzymes [30]. We identified similar concentrations of the arbuscular mycorrhizae marker 16:1 ω5c for
Idiosyncratic effects of tree species coupled with extreme changes in water input contribute in shaping the soil microbial community structure and enzymatic activity at the Guánica Dry Forest. In this system, saprophytic fungi, arbuscular mycorrhizae and anaerobic Gram-positive sulphur-reducing bacteria seem to be more sensitive to rainfall pulses when compared to Gram-positive (including actinobacteria), Gram-negative and protozoan communities. Even though monthly variations play a significant role in microbial community structure, soil enzyme activities did not vary during the months sampled. Our findings demonstrate that, although mesoclimate is a determinant driver of ecosystems, tree species is a stronger modulator of the soil microbial dynamics at the Guánica Dry Forest. To our understanding, this is the first study that provides insight into the soil microbial community of the Guánica Dry Forest, a valuable contribution that will help elucidate strategies for better management and protection of the soil biota of the area.
\nThe project was funded by NSF Grant HRD-0734826 and is a contribution of the Centre of Applied Tropical Ecology and Conservation of the University of Puerto Rico in collaboration with the USDA-ARS in Lubbock, Texas. We appreciate the support of Mr. Larry Diaz, laboratory coordinator, and students from the Ecosystems Processes and Function laboratory of the University of Puerto Rico, Río Piedras Campus, that participated in the field collection of soil samples; Mr. Jon Cotton, laboratory technician, and students from Dr. Veronica Acosta-Martinez laboratory at the USDA-ARS Lubbock Texas, that participated in sample processing, and Mr. Dean Holder from the USDA-ARS Lubbock Texas, who offered training in the determination of soil texture. Special thanks to Dr. Francisco Calderon from the USDA-ARS Akron Colorado for kind participation in the revision of this manuscript. Thanks to Dr. Raymond Tremblay for commenting on the statistical part of the manuscript.
\nNo conflict of interest.
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All published Book Chapters are licensed under a Creative Commons Attribution 3.0 Unported License. Monographs are licensed under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) license granted to all others. Our Copyright Policy aims to guarantee that original material is published while at the same time giving significant freedom to our Authors. IntechOpen upholds a flexible Copyright Policy meaning that there is no copyright transfer to the publisher and Authors hold exclusive copyright to their work.
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