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Molecular Study for Diagnosis of Ureaplasma parvum in Women with Recurrent Miscarriage
The objects of study is concerted to investigate the occurrence of Ureaplasma parvum in women with recurrent abortion and to determine the distribution of U. parvum serovars (1, 3, 6, 14) in women with recurrent abortion by conventional polymerase chain reaction (PCR) technique. In total, 130 samples included vaginal bleeding, vaginal swab, and urine, were collected from women with recurrent abortion and 40 samples included vaginal swab and urine from control women without recurrent abortion. Through the study, two types of media were used, Ureaplasma broth (IH Broth) and Ureaplasma agar (IH Agar). The positive isolates for Ureaplasma spp. were investigated by conventional PCR technique for identification of U. parvum and subtyping to their serovars (1, 3, 6, 14). The results revealed the U. parvum was identified in 29.6% from patient group and 11% from the control group. U. parvum isolates were further subtyped by using PCR, the results showed the serovar 3 was the most frequent isolate in proportion (42.8%), whereas serovar 1 (28.5%), serovar 6 (14.2%), and serovar 14 (14.2%) in patient group but in the control group only serovar 1 was isolated in rate (11%). These results evidently indicate that U. parvum may be an important etiologic agent for recurrent abortion.
Part of the book: Obstetrics