Present and future installed electricity capacity by energy sources (MW) in Turkey.
\r\n\t
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Before his doctorate, he has worked as a researcher at the universities in U.S.A., France and Canada. As a technologist, he worked at the Paharpur Cooling Towers Ltd. India, which is worlds' leading process cooling equipment manufacturer. He owns a patent, a member of several scientific communities, editor of international journal, has written and presented more than twenty research articles, book, and is serving as reviewer to several international journals. 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A variety of noble metal nanostructures encapsulated or coated with biopolymers have been studied by researchers in the field of nanobiomedicine. This is due to the interesting properties and wide-spectrum application of biopolymer-based nanomaterials. These materials have been shown to combine both the intrinsic features of noble metal nanostructures and the biological features presented by renewable source polymers [1–3]. They have been found not only to exhibit excellent imaging properties but also to efficiently deliver various drugs and therapeutic genes [4–7]. Noble metal nanostructures used in therapy and diagnosis must be non-toxic, stable in biological media and should be specific for the target [8,9]. However, the requirement of these three factors has hindered the use of many noble metal nanostructures in a variety of biomedical applications. Hence, there is a need for the conjugation of these materials with functional biological molecules to necessitate and improve their efficacy for biomedical functionalities. Bionanocomposites exhibit better biomedical values than their naked nanoparticle counterparts. They demonstrate colloidal stability, maintain plasmonic properties and show little or no effect on cell viability in the biological cell system [10,11].
Generally, the synthesis of most noble metal nanostructures requires a high concentration of surfactant, which directs the asymmetric geometry. However, studies have shown that surfactants tend to degrade biological membranes, thus raising significant concern about the cytotoxicity of these materials [12,13]. The cytotoxicity of these surfactant-bound nanostructures can be reduced by minimizing the surfactant concentration below the critical micellar concentration. This reduction is effected at the expense of the stability of the nanomaterials solution, and as a result, their unique optical properties in biological environments are compromised.
There have been calls for the development of nanomaterials based on the principles of green chemistry and, consequently, a myriad of studies have emerged. Some of the proposed solutions are based on the substitution of toxic reagents with more eco-friendly materials. As a result, a lot of research has been done on developing more environmentally benign synthetic methods for noble metal nanomaterials owing to their wide area of applications. In addition, there have been calls for the development of sustainable process and practices in order to define products as green based.
Raveendran and co-workers [14] report three main steps to be considered in the green synthesis of metallic nanoparticles. These are (i) the choice of solvent medium used for the synthesis, (ii) use of environmentally benign reducing agent and (iii) the choice of non-toxic material as stabilizing agent. The use of environmentally benign materials for the synthesis of metal nanoparticles provides numerous benefits ranging from biocompatibility, renewable feedstocks, cost-effectiveness, waste prevention, amenable scale-up, synthetic steps reduction, use of safer solvents and increase of energy efficiency to eco-friendliness [15–17].
Nanomaterials possess phenomenal ability to create better materials as they are currently being used in numerous products and industrial applications (Figure 1). Most of these materials present evolutionary development of existing technologies. The use of biomaterials such as ascorbic acid, glutathione, sugars, glycerol, orange peel, plant extracts and yeast, as alternative raw materials for synthesizing nanomaterials presents an even more fascinating approach, thus impacting their significance [18,19]. In the area of nanomaterial research, noble metal nanostructures have received tremendous attention due to their wide range of applications in electronics, sensing, catalysis, photonics, environmental clean-up, imaging, water purification, cancer therapy, labelling and drug delivery [20–23]. Most notable among the breakthrough applications of biopolymer-based noble metal nanostructures include: the use of biopolymer-based biodegradable food-packaging materials in the place of non-biodegradable plastics [24], nanocrystal metal–chitosan granular composite material for water purification [25], selective tumour ablation using polymer-coated gold nanorods and metal nanostructure for sensing applications [26–28].
Nevertheless, with noble metal nanostructures having such a phenomenal advantage, there also exist limitations to their application. There have been increasing concerns relating to their toxicity arising from their bioaccumulation in human body. It has been reported that they preferentially accumulate in the liver and spleen [29,30]. As a result, they cause many dysfunctions to these organs causing disruption to their body activities. The nanoparticles size, shape and surface functional groups play a vital role in their toxicity effect. There exist a challenge of conjugating nanoparticles to the surface of metal nanoparticles with negligible or no toxicity effect. As the nanoparticles become conjugated, the surface chemistry, size and shape tend to change, thus influencing their cell and protein interface interactions. This inevitably leads to unspecific and non-selective activities in the body [31].
Applications of nanoparticles [
Nanoparticles of gold, silver and platinum are classified as noble metal nanostructures. They are nanosized metals with at least one dimension within the nanometre size range of 1–100 nm. Unlike bulk metals, which are typically ductile and possess high thermal and electrical conductivity, metal nanoparticles completely differ in such physical properties. The properties exhibited by metal nanoparticles are completely different from those exhibited by their corresponding bulk metals due to the absence of electron delocalization [32]. Noble metal nanoparticles have very large surface-area-to-volume ratio when compared to their bulk counterparts, thus making them attractive for a wide array of many applications. Their enhanced properties can be tailored by controlling their shape, size and composition [33,34]. A new generation of hybrid nanostructured materials presents an emerging field in nanotechnology. Nanobiocomposite over the past few years has become a term used to designate composites, which contain naturally occurring polymers (biopolymers) and an inorganic structure. The development of nanocomposites comprising a biopolymer matrix with noble metal nanostructures has been extensively studied and considerable efforts are now being directed at biopolymer-based nanocomposites with improved properties [25,26].
Nanoparticle synthesis over the past decades has received enormous attention due to their extensive applications. Today, researchers have been able to controllably synthesize noble metal nanoparticles of various shapes and dimensions. These include zero-dimensional, one-dimensional, two-dimensional and three-dimensional nanostructures in a remarkable fashion [35–40]. Noble metal nanoparticles have been synthesized using various methods. Some of these methods include the electrochemical method [41], photochemical method [42], chemical reduction method [33], sonochemical method [43] and the biosynthesis method [44]. A universal reflection on the environmental risks or hazards and sustainability arising from most of these methods other than biosynthetic routes prompted the need for greener approaches in the manufacture of these materials. As a result, considerable attention has been focused on the biosynthesis as the method of choice for the synthesis of these nanoparticles. Metal nanoparticles have been embedded in a host biopolymer matrix in a variety of ways, which include
Interesting biopolymers have been used to synthesize gold, silver and platinum nanoparticles via direct
The use of sodium citrate in the synthesis of gold and silver nanoparticles is perhaps the first method to be developed [46] and it is still relevant today. It is a simple process for reducing metal salts to nanoparticles with modest monodispersed spherical shape. The particle diameters are usually within the range of 10–20 nm. Colloidal gold and silver nanoparticles are produced by this technique because the citrate ions act as both capping and reducing agents. Following this trend, spherical silver nanoparticles with monodispersed and controlled sizes have also been effectively synthesized. In addition, monodispersed gold nanoparticles with controlled size have also been reported. This is achieved by altering the concentration of gold chloride ions with respect to the citrate [47]. Furthermore, Bastus and co-workers [48] report the enhanced citrate-reducing ability in combination with tannic acid. The tannic acid is instrumental to the formation of the seeds and subsequent high yield of the final spherical particles.
The most common chemical reduction method for preparing noble metal nanoparticles involves the reduction of their metal salts or precursor (from M+n to M0) using sodium borohydride as the reducing agent. Sodium borohydride is a strong reducing agent in organic and inorganic chemistry, and it is used to reduce most transition metal ions to zero-oxidation metal nanoparticle state M(0) in the presence of a colloidal stabilizer [49]. The stabilizing agent prevents the aggregation of the nanoparticles after formation. NaBH4 reduces metal precursors by hydride transfer. However, single-electron transfer is also a possibility, given the electron-rich nature of borohydride anion. Sodium borohydride can also act as bi- or tridentate ligands [50]. It has also been demonstrated that NaBH4 has the ability to control the size and morphology of nanoparticles to a large extent using appropriate synthetic techniques. As a result, nanoparticles with very large surface area relative to volume have become feasible. This is particularly important because the surface plasmon resonance (SPR) band of nanoparticles is a function of morphology [51,52]. The concentration of NaBH4 has been found to affect the size, shape, dispersity and even hydrodynamic diameter of the metal nanoparticles. As the concentration of borohydride increases, the aspect ratio (length divided by width) of the nanorods formed decreases. Moreover, at a certain higher concentration, shape deformation occurs. Also, ageing the sodium borohydride solution prior to use has been found to influence the shape formation of the nanorods [53]. Monodispersed Ag-NPs have also been synthesized using borohydride reduction of silver salt. The borohydride anions are adsorbed onto silver nanoparticles and aggregation is prevented by polyvinyl pyrrolidone (PVP) addition. A large excess of the reductant is also needed to stabilize the nanoparticle product [49] since they are adsorbed onto silver nanoparticles after they are formed.
THPC is an exceptionally strong reducing agent capable of synthesizing smaller gold or silver nanoparticles usually used as seeds for the formation of nanoshells [54] and gold nanoclusters employed for fluorescence imaging [55,56]. Typically, it serves as a good reducing agent in alkaline solution. However, to achieve good nanoparticle stability, a stabilizer is often added alongside with THPC during synthesis. Nonetheless, Hueso and co-workers have reported the use of THPC as both reducing agent and stabilizing ligand in the synthesis of ultra-small noble metal nanoparticles. This is achieved in a single-step room-temperature method with a wide variety of monometallic and bi/tri nano alloys as products [57]
Polysaccharides are long polymers of monosaccharide sugars and their derivatives. Unlike proteins or nucleic acids, these polymers can either be straight chained or branched. They can be of one type of monosaccharide (homopolysaccharides), or more than one (heteropolysaccharides) in repeating units. Polysaccharides can also be divided into groups according to their two major functions: contribution to structural components of cells and energy storage. Cellulose is a structural polysaccharide while starch is mainly an energy storage polysaccharide. Starch is the main energy storage in plants while glycogen is the main energy storage in animals. Both starch and cellulose are polymers of glucose but they differ based on the configuration of C–O bonding [58]. They are generally insoluble in cold water, although at around 100 oC, starch is water-soluble while cellulose needs a temperature higher than 300 oC to be water-soluble [59]. Upon hydrolysis with acids or enzymes, they eventually yield their constituent monosaccharide sugars. Polysaccharides have the ability to coordinate metal ions and thus act as stabilizing agents. Furthermore, they have been known to exhibit reducing properties in the synthesis of metal nanostructures. This dual role has enabled the production of metal nanoparticles with improved properties and functionalities such as different shapes and sizes, hydrophilicity, biocompatibility, specificity and non-toxicity. Due to their non-toxic nature, polysaccharides are recognized as green reducing agents for nanostructure synthesis. Their structural hydroxyl groups provide them with strong reducing ability and solubility in water. Other natural polysaccharides such as chitosan have also gained attention in the preparation of nanoparticles [60]. Under this section, we will review the use of starch, cellulose and dextran for the synthesis of noble metal nanoparticles.
Chemically, starch consists of long chains of D-glucose (a monosaccharide) units joined by 1,4-glycosidic bonds. They are formed in plants during photosynthesis. They are present in many plant-based food sources such as root vegetables, for example, potatoes and cereals. The eco-friendly biosynthesis of metal nanoparticles using starch has been well reported. Most of them have been performed through reduction processes. It is a simple method which yields high amount of nanoparticles and to some extent allows for control of particle size. El-Rafie and co-workers [61] report the use of alkali-treated maize starch as both reducing agent and stabilizer for the production of silver nanoparticles. In their report, a redox reaction occurs between AgNO3 and alkali-treated starch to obtain the nanoparticles. The nanosilver obtained is spherical in shape with particle size ranging between 4 and 6 nm. Valencia and co-workers [62] also report the use of starch as reducing agent at 90 oC in the presence of NaOH. Spherical-shaped Ag-NPs with particle diameter between 10 and 30 nm were produced. Furthermore, the role of pH in the green-mediated synthesis of starch-capped Ag-NPs has been investigated. Ag-NPs are synthesized using two green materials: glucose as the reducing agent and starch as the stabilizing agent. The presence of an accelerator (NaOH) is found to affect the size, size distribution and the pH of the solution of the as-synthesized Ag-NPs. At mildly acidic conditions, occurrence of starch hydrolysis affects the particle size. The insufficient protection of the particle surface led to the formation of larger particles but with defined sizes. However, less starch hydrolysis results in nanoparticle aggregation leading to a blue shift and splitting of the spectra [63]. Spherical nanoparticles and nanowires of gold and silver with various sizes and shapes have also been prepared using a monosaccharide (D-glucose) and a polysaccharide (soluble starch) [64].
Gold nanoparticles coated with different sugars are successfully synthesized using a non-toxic, water-soluble phosphine amino acid (THPAL) as a reducing agent. The sugars used are glucose (monosaccharide); sucrose, maltose or lactose (disaccharides); raffinose (trisaccharide) and starch (polysaccharide) [65]. The capping ligand plays a vital role in transforming the shapes of the nanoparticles formed. Platinum nanoparticles (Pt-NPs) with uniform particle size (2–4 nm) have also been synthesized using soluble starch. The soluble starch performs a dual role as both reducing and stabilizing agents. Under alkaline treatment, the degraded intermediate species with reducing potentials (i.e., small molecules of aldehydes and hydroxyl ketones) generated
Cellulose chemically consists of long chains of six-membered ring glucose (a monosaccharide) molecules. The repeating units comprise two anhydroglucose rings (C6H10O5)
Dextran are water-soluble branched polysaccharides of glucose (dextrose) produced by lactic acid bacterial action on sucrose. The linear chains are linked by α-1,6 glycosidic bond between glucose molecules while the branches begin from α-1,3 linkages. In this method, metal nanostructures are prepared using an environmentally benign solvent and dextran as capping agent, or in some cases dextran serves as both reducing and capping agents. Wang and co-workers [75] report the synthesis of well-dispersed, uniform and biocompatible gold nanoparticles at room temperature using dextran as both reducing and stabilizing agent. The as-synthesized dextran-coated Au-NPs are stable in high ionic strength medium, thereby making them important materials for fabricating novel nanostructures.
Environmentally friendly copolymers of dextran have also been used to synthesize gold-copolymer and silver-copolymer nanoparticles. The simple green synthetic method makes use of graft copolymers, dextran-graft-poly(ε-caprolactone) and dextran-graft-poly(δ-valerolactone) as reductants and stabilizers in the synthesis of the noble metal nanoparticles. The amphiphilic nature of the copolymer is crucial during the process, as this allows interaction of the copolymer with the metal surface and the aqueous medium. The OH end groups of the grafted blocks of copolymers are responsible for the reduction of gold and silver ions [76,77].
UV–Visible spectrophotometry is an essential powerful tool for characterizing the optical properties of various materials at molecular and atomic levels. Typically, gold and silver nanoparticles exhibit a UV–Visible characteristic absorption band peak around 520 and 400 nm, respectively, due to their SPR properties [78–81]. The SPR is due to the collective oscillation of light-excited free-moving conduction band electrons present at the surface of the noble metals. Stabilizing these metals with polysaccharides usually causes a change in the electron density of the metals and thus a shift in the positioning of their SPR peaks. However, the direction and the actual positioning of SPR peak depend on the type of nanoparticle [79,80], particle size and structure of polysaccharide involved [82–84].
FTIR is used to identify the functional groups present in the polysaccharide used to stabilize metal nanoparticles [80]. In conjunction with UV–Visible, dynamic light scattering and Zeta potential data, FTIR can be used to evaluate the extent of polysaccharide stabilization of metal nanoparticles (NPs). Stereotypically, polysaccharide-stabilized gold or silver NPs usually show absorption wavenumber bands for O–H (3500–3200 cm-1: alcohols; 3300–2500 cm-1: acids), C–H (3000–2850 cm-1: saturated alkanes) and C–O (1320–1000 cm-1: alcohols and ethers) groups associated with general carbohydrate structure [79,80,83,85]. To ascertain the effectiveness of stabilization of nanoparticles by the polysaccharide, shifts in the affected groups are usually observed. The hydroxyl, hemiacetal and aldehyde groups play significant roles in the synthesis and stabilization of polysaccharide-capped silver [85,86] and gold nanoparticles [79,83].
The zeta potential measures the overall charge on polymer-stabilized noble metal nanoparticle system [87] as either negative or positive in millivolts. As mentioned earlier, it may be used in conjunction with FTIR to assess the extent of nanoparticle stabilization by polysaccharides. When stabilized with carboxylic acid-terminated polysaccharides, gold and silver NPs are usually surrounded by negative charges. The negatively charged polysaccharide molecules interact with one another via electrostatic repulsion and thus help to prevent agglomeration. Similarly, positively charged polysaccharide-stabilized gold and silver nanoparticles are stabilized via electrostatic repulsion of the polysaccharide’s molecules. The higher the magnitude of the zeta potentials, the greater is the extent of charge-to-charge electrostatic repulsion and thus the greater is the stability of nanoparticles [82] and vice versa. The negatively or positively charged polysaccharide-stabilized silver nanoparticles are important for efficient antimicrobial efficacy of silver nanoparticles against gram-positive and gram-negative microbes, respectively [82].
The dynamic light scattering, also known as the photon correlation spectroscopy, uses light scattered by moving particles in a dispersible solution system to determine the hydrodynamic size distribution of various particles present in the solution. DLS particle-size estimation is based on measurement of the intensities of scattered lights by various particles present in the solution, provided the particles’ movement is Brownian in nature. Therefore, it is used to evaluate the homogeneity of a solution and thus the aggregation of nanoparticle in the solution. For a reliable DLS measurement, a polydispersity index of between 0.05 and 0.7 may be preferable. Also, the refractive indices (
TEM is a two-dimensional technique used to probe the shape and morphology of a nanoparticle system [83,88]. The shapes of nanoparticles affect the optical, chemical and biological properties of metal nanoparticles. For example, silver nanoparticles have been shown to evolve different shapes as temperature increases when synthesized and stabilized by methyl cellulose [89]. This change in shape morphology of stabilized silver nanoparticles was observed using the TEM technique (Figure 2). Furthermore, TEM can be used to estimate the particle-size distribution of nanoparticle systems [86]. The particle-size data obtained from TEM are usually compared to the information obtained from other particle-size measurements such as DLS and X-ray diffractometry for analytical reliability and accuracy. Furthermore, TEM can be used in conjunction with FTIR, UV–Vis and other probing techniques to elucidate the mechanism of evolution of nanoparticles during the course of the synthesis (Figure 3).
SEM is similar to TEM but differs in its magnifications and image outputs. SEM is a three-dimensional imaging probe, which can be used to visualize the three-dimensional images of stabilized nanoparticles [80,90,91] (Figure 2). ICP-MS is mainly used to determine the concentration of metals in a material. Thus, it can be used to estimate the concentration of gold and silver nanoparticles [92] within the surrounding structure of polysaccharide stabilization. The amount of nanoparticles present within a stabilizing matrix is essential for such sensitive applications such as environmental sensing, industrial catalysis and biomedical diagnostics and therapies. The powder XRD technique is used to probe the extent of the crystallinity of both unstabilized and stabilized nanoparticles. Particles size can also be estimated using XRD technique. Usually, a face-centred cubic structure is observed for polysaccharide-stabilized gold [79,83,93] and silver nanoparticles [82,90].
A: TEM image of methyl cellulose-capped silver nanoparticles at 60 °C [
Elucidation of a reaction mechanism using UV–Vis and TEM. a: UV–Vis absorption spectra of Ag-NPs at different reaction times during the synthesis of Ag-NPs. b: TEM image of Ag-NPs (at 1 h reaction time) showing necklace arrangement. c: TEM image at 24 h reaction time, scale bar, 10 nm (inset shows high-resolution image). d: TEM image at 30 h reaction time, scale bar, 20 nm (inset shows high-resolution image and particle-size distribution) [
Gold nanoparticles are essential nanomaterials for many biomedical applications such as targeted biological sensing [94], site-specific drug delivery, diagnostic imaging [84,95,96] and photothermal therapy [95,97]. They are essentially utilized for these applications due to their unique SPR property, inertness [84,96], non-toxicity, enhanced permeability retention effect, conjugatable surfaces for the attachment of targeting [98] and therapeutic agents [99], near-infrared light absorption [97], high light absorption [95] and scattering properties and generation of therapeutic heat after light irradiation [95]. Recent advances in the use of gold nanoparticles have focused on the use of polysaccharide-stabilized gold nanoparticles. One of the reasons for this is the better efficiency of polysaccharides in synthesizing and stabilizing gold nanoparticles compared to other stabilizing agents such as oligosaccharides or monosaccharides [100]. Other reasons are their low toxicity, easily conjugatable functional groups [101] and good biocompatibility [102,103]. Some of the recent biomedical applications of gold nanoparticles are provided in the next section.
Various structures of gold nanoparticles such as the nanospheres, nanorods and nanocages have been shown to exhibit different SPR characteristics [78,103], which can be used to detect biomarkers [104] and pathogens in body fluid samples. In targeted plasmonic-assisted biosensing, stabilized gold nanoparticles are conjugated to some specific biomolecule targeting agents such as carbohydrates [102,105] peptides [106], antibodies and oligonucleotides [102] which react specifically with some disease biomarkers or microbes. This interaction often leads to the aggregation of gold nanoparticles [104] which causes shifting of the surface plasmon resonance peak. This shift effect can be detected by visual colour change, spectrophotometric measurement or dynamic light scattering techniques. Targeted plasmonic biosensing could be successfully employed for the rapid improvement of various diagnostic techniques such as homophase immunoassay, DNA assay and enzymatic assay [104].
Gold nanoparticles are plasmonic entities which absorb and scatter light from the visible to near-infrared optical region where tissues are relatively transparent to light [106,107]. These properties enable them to be used as contrasting agents in many biomedical imaging applications such as magnetic resonance imaging and computed tomography [108]. When excited with near-infrared light, gold nanoparticles absorb and scatter light more than the surrounding tissues leading to a better detection of their sites of localization. In targeted magnetic resonance imaging (MRI) technique, stabilized gold NPs are conjugated to an active targeting material such as a specific site-targeting carbohydrate [105], peptides [106,109,110], aptamers [108] or external magnetic field-driven iron oxide nanoparticles (IONPs) [111] in order to achieve active site-targeting, detection and better imaging. Superparamagnetic iron oxide nanoparticles (SPIONs) are excellent MRI contrast agents [112,113]. Thus, utilizing stabilized gold-SPION hybrids, such as SPION-gold core–shell, promotes greater improvement of the MRI technique. Furthermore, gold NPs may be coupled with conventional imaging dyes or contrasting agents to improve their imaging efficacies.
In targeted fluorescence detection, stabilized gold nanoparticles are either bonded directly [98] or conjugated to a fluorescence molecule [95] bonded to an active site-targeting biomolecule [110]. The technique makes use of (i) the synergetic properties of the selective localization of the gold nanoparticles in disease tissues, (ii) interaction of the active targeting biomolecules with their corresponding disease tissue receptors and (iii) emitted fluorescence light from the gold nanoparticles [98] or conjugated fluorophore to improve detection of disease tissues [110]. For diagnosis purposes, the active site targeting gold nanoparticle solution may be injected via intravenous means and the body exposed to the fluorescence spectroscopy imaging technique. The diseased tissues containing the gold nanoparticle fluorophore conjugates will emit light of specific wavelength while the healthy tissue remains non-fluorescent [95,110].
Targeted PTT involves the use of active site targeting stabilized gold nanoparticles (Au-NPs) for specific tumour cell destructions. The Au-NPs generate therapeutic heat after irradiation with light of appropriate wavelength and dose. The site targeting agent interacts with the tumour cell surfaces in order to pave way for the nanoparticles to gain entrance into the cell where the therapeutic function will take place. Especially suitable for this purpose are the gold nanorods [78,114] and nanoshells [78,115] whose aspect ratio and the ratio of the shell to core diameter can be tuned to absorb light in the near-infrared regions where biological tissues are transparent to light. Gold nanorods have been extensively applied for the eradication of cancer cells via PTT [102]. Other gold nanoparticle structures that can be used for PTT are gold nanocages [78,91]. However, all gold nanostructures exhibit different PTT efficacies due to their different structures and surface plasmon resonance properties.
Stabilized silver nanoparticles (Ag-NPs), being a plasmonic entity also exhibit biological imaging characteristic [96,116] and plasmonic and fluorescence detection of biomolecules [116,117,118] and toxic heavy metals [119,120]. Like gold nanoparticles, it could be used to improve image contrast of disease tissues when functionalized or employed as nanocarriers of specific site-targeting agents such as peptides, antibodies and carbohydrates which show some specific affinity towards certain molecules present in disease tissues. As nanocarriers of these biomolecules, silver nanoparticles may cause enhanced biological image contrast via their light absorption and scattering properties. However, the choice of silver nanoparticles for fluorescence imaging function may be more for silver nanoclusters (Ag-NCs) compared to other silver morphologies due to their small size-dependent fluorescence effect. It has been reported that as the size of silver nanoparticles approaches the dimension of the Fermi wavelength of an electron, they emit fluorescence light which can be tuned from the visible to near-infrared region after suitable light irradiations [116]. Moreover, Ag-NCs have also been found to be useful as an excellent nanobiosensor agent for the detection of biotoxins [118] and toxic inorganic heavy metals [120]. Nonetheless, like gold nanoparticles, the interaction of functionalized, stabilized silver nanoparticles with disease biomarkers such as proteins may cause aggregation and thus a shift in the position of their SPR band which may be used to detect these biomarkers.
Silver nanoparticles are especially known for their antibacterial activities [90,121–125]. However, in recent years, reports about the antiviral effects of silver nanoparticles are beginning to emerge [126–128]. In their study, Gusseme and co-workers [128] employed biogenic silver nanoparticles to remove murine norovirus 1 (MNV-1) from drinking water. The biogenic silver was mixed with a water filter to effect the viral removal. Also, the use of silver nanoparticles in preventing the binding of HIV-1 to human host cells via binding of nanoparticles to the virus’ glycoproteins
Silver nanoparticles could also function as anti-angiogenesis agents by preventing the formation of new blood vessels responsible for the development of many human diseases such as cancer, macular degeneration and inflammatory diseases [133]. In their study, Gurunathan and co-workers [133] suggested that silver nanoparticles readily interfere with the angiogenesis pathway by inhibiting the activation of p13k/akt.
Biopolymers have continued to revolutionize the advancements of green nanotechnology. Nanoscale derivatives of polymers like starch, cellulose and dextran can be synthesized on a large scale and can be used for the manufacture of bionanocomposites. They present promising alternatives to the environmental challenging non-biodegradable plastics via industrially viable process. Most of these biopolymers also offer a route for completely green synthesis of several noble metal nanoparticles performing the dual role of stabilizing and reducing agents in aqueous medium. Biopolymer-mediated synthesis offers numerous advantages which include efficient solubility in water, non-cytotoxicity and biocompatibility in biomedical applications. Polysaccharide-capped silver and gold nanoparticles are emerging as excellent nanomaterials in many fields, including biomedical and environmental. This is due to the result of the excellent functionalities inferred on them by the polysaccharide molecules. These properties include long-term stability and high solubility in aqueous solution, specific biomolecule targeting which induces specific therapeutic functions and toxicity reduction.
The Energy Market Regulatory Agency (EMRA) has license fee exemption for renewable energy investors and the Turkish Electricity Trading Company, TETAS, can provide buying guarantees to renewable energy, further supporting inward investment.
In Turkey, present and future installed electricity capacity by energy sources is given in the Table 1. According to the projection, the share of hydro capacity will be highest with 37.4% and share of natural gas will be 32.6%. Renewable energy supply for at the present and in the future in Turkey is given in the Table 2 [1, 2, 3, 4, 5, 6, 7] and the hare of sources in electricity generation in 2020 is given ın the Figure 1.
Energy source | 2010 | 2012 | 2014 | 2016 | 2018 | 2020 |
---|---|---|---|---|---|---|
Lignite | 8280 | 8280 | 8280 | 8280 | 8280 | 8280 |
Hard coal | 560 | 560 | 560 | 560 | 560 | 560 |
Imported coal | 1840 | 3470 | 4680 | 4690 | 4690 | 4690 |
Natural gas | 14,840 | 17,560 | 18,400 | 18,400 | 18,400 | 18,400 |
Geothermal | 90 | 90 | 90 | 90 | 90 | 90 |
Fuel oil | 1900 | 1900 | 1900 | 1900 | 1900 | 1900 |
Other | 29,382 | 33,920 | 35,970 | 35,980 | 35,980 | 35,980 |
Hydropower | 16,393 | 19,880 | 19,880 | 21,094 | 21,094 | 21,112 |
Total (MW) | 45,255 | 53,320 | 55,370 | 56,594 | 56,594 | 56,612 |
Present and future installed electricity capacity by energy sources (MW) in Turkey.
Renewable energy sources | 2000 | 2005 | 2010 | 2015 | 2020 |
---|---|---|---|---|---|
Hydropower (ktoe) | 2656 | 4067 | 4903 | 7060 | 9419 |
Geothermal, solar and wind (ktoe) | 978 | 1683 | 2896 | 4242 | 6397 |
Biomass and waste (ktoe) | 6457 | 5325 | 4416 | 4001 | 3925 |
Renewable energy production (ktoe) | 10,091 | 11,074 | 12,215 | 15,303 | 19,741 |
Share of total domestic production (%) | 38 | 48 | 33 | 29 | 30 |
Share of TPES (%) | 12 | 12 | 10 | 9 | 9 |
Hydropower (GWh) | 30,879 | 47,287 | 57,009 | 82,095 | 109,524 |
Geothermal, solar and wind (GWh) | 109 | 490 | 5274 | 7020 | 8766 |
Renewable energy generation (GWh) | 30,988 | 47,777 | 62,283 | 89,115 | 118,290 |
Share of total generation (%) | 25 | 29 | 26 | 25 | 25 |
Geothermal, solar and wind (ktoe) | 910 | 1385 | 2145 | 3341 | 5346 |
Biomass and waste (ktoe) | 6457 | 5325 | 4416 | 4001 | 3925 |
Renewable total consumption (ktoe) | 7367 | 6710 | 6561 | 7342 | 9271 |
Share of total final consumption (%) | 12 | 10 | 7 | 6 | 6 |
Renewable energy supply in Turkey.
Share of sources in electricity generation in 2020.
In the last decade a lot of dams and small hydropower have been planned to build in Turkey in Eastern Black Sea and Sakarya Basins also. Most of them have been already completed (for example Deriner Dam 669.60 MV in Artvin) and others under construction (for example Yusufeli Dam 558 MV in Yusufeli) in the eastern Black Sea region. Similarly, Adasu Small Hydropower (10 MV) has been completed and Akçay Dam (12 MV) under construction in Pamukova in Sakarya Basin.
On the other hand, in the last two decades a lot of dams and small hydropower (SHP) either completed or under construction in Turkey. State Hydraulic Works (DSI) provided equipment for the 140 MW Kigi project in Turkey, including three 46.6 MW turbines with pre-turbine gate valves, three generators of the same capacity, switchgear, and all other equipment for the turbine hall [8].
Figures 2 and 3 show hydropower global capacity, shares of top six countries and hydropower capacity and additions, top six countries for capacity added, 2013 respectively [8, 9].
Hydropower global capacity, shares of top six countries, 2013.
Hydropower capacity and additions, top six countries for capacity added, 2013.
There are different methods for calculating hydroelectric energy. Since the amount of energy depends mostly on the water drop and water discharge values it is decided to use the method which is mentioned from some literatures [10, 11]. The method and it equations as follows:
The equation, N = γ*H*Q , N = 9.81*H*Q* ր (ր = 0.85–0.92) can be written for N and ր is 0.85 the N is calculated as:
and then E is calculated as:
In these equations:
N = Power (tm/s), (1 tm/s= 9.81 kW = 13.3 HP) and it goes to (kW, MV, GW)
γ = Specific weight (t/m3)
H = Water drop (head of water) (m)
Q =Water discharge (m3/s)
E = Energy value of water resource (kWh, MWh, GWh)
Out of 26 main basins, Sakarya and Eastern Black Sea Basins are very important about hydro energy potential in Turkey.
In 2016, Sakarya and Eastern Black Sea Basins have 63.358 km2 and 22.845 km2 area and 5.16 km3 and 16.46 km3 annual average flow respectively.
In this study, some streams which are observed their water discharge and the others hydraulics and hydrological characteristic were selected in the Sakarya and Eastern Black Sea Basins [12, 13].
When considered the alternative energy resources, hydroelectric generation is not generally achieved in Turkey, especially in Sakarya and Eastern Black Sea Basins. However, with some laws and regulations enacted, in many activities and opportunities such as construction, production and distribution related to investments carried out exclusively by the Turkish Government, but private sectors have not used enough these opportunities yet. Therefore, by using these facilities well, it will be possible for the private sector to close the energy gap in Turkey or to minimize it by evaluating the river resources in the region [14]. In this study, some small streams have been selected and their hydro energy potential determined for Sakarya and Eastern Black Sea Basins in Turkey.
There are three types of potential which are gross (theoretical) potential, technical potential and economical potential [12, 15, 16].
Economical potential in generally depends on some geological and technical conditions such as geographical structure of basin, technical team and equipment facilities etc. To calculate of hydropower potential for selected streams in the Sakarya and Eastern Black Sea Basins the equations 1, 2 and 3 are used.
Hydroelectric Power (N) and hydroelectric energy (E) have been calculated by using this method. Hydroelectric power and hydroelectric energy values for selected streams in the Sakarya and Eastern Black Sea Basins are given in the Tables 3 and 4 respectively.
Stream and station name | Water discharge (Q), m3/s | Water drop (head of water) (H), m | Hydroelectric power (kW) | Hydroelectric energy (GWh) |
---|---|---|---|---|
Sakarya River - Dogancay | 121 | 41 | 4217 | 364 |
Kocasu - Rustumkoy | 17.9 | 198 | 3013 | 260 |
Sakarya River - Aktas | 7.19 | 837 | 5115 | 442 |
Aladağ Stream - Karakoy | 13.3 | 505 | 5709 | 493 |
Mudurnu Stream - Dokurcun | 7.75 | 286 | 1884 | 163 |
Total | 19,938 | 1722 |
Hydroelectric power and hydroelectric values for selected streams in the Sakarya Basin.
Name of selected small stream | Average water discharge (m3/s) | Average water drop (altitude) (m) | Hydroelectric power (kW) | Hydroelectric energy (GWh) |
---|---|---|---|---|
Iskefiye Stream | 1.3 | 535 | 5.564 | 48.74 |
Sana Stream | 1.5 | 455 | 5.460 | 47.83 |
Orta Stream | 1.05 | 285 | 2.394 | 20.97 |
Horyan Stream | 1.12 | 230 | 2.061 | 18.05 |
Canakci Stream | 2.36 | 405 | 7.646 | 66.98 |
Yanbolu Stream | 4.90 | 60 | 2.352 | 20.60 |
Kalyan Stream | 2.45 | 135 | 2.646 | 23.18 |
Fol Stream | 2.91 | 155 | 3.608 | 31.61 |
Kucuk Stream | 2.34 | 110 | 2.059 | 18.04 |
Durana Stream | 1.71 | 90 | 1.231 | 10.79 |
Kadiralak Stream | 2.90 | 135 | 3.132 | 27.44 |
Manahoz Stream | 4.98 | 65 | 2.590 | 22.68 |
Baltaci Stream. | 5.72 | 75 | 3.432 | 30.06 |
Altıntas Stream | 4.11 | 95 | 3.124 | 27.36 |
Acisu Stream | 1.56 | 155 | 1.934 | 16.95 |
Akhisar Stream | 1.60 | 105 | 1.344 | 11.77 |
Solakli Stream | 2.13 | 105 | 1.789 | 15.67 |
Holo Stream | 2.65 | 95 | 2.014 | 17.64 |
Askaroz Stream | 6.26 | 105 | 5.258 | 46.06 |
Kokasor Stream | 4.03 | 105 | 3.385 | 29.65 |
Pilahoz Stream | 2.50 | 95 | 1.900 | 16.64 |
Potomya Stream | 0.84 | 145 | 974 | 8.54 |
Hako Stream | 0.83 | 105 | 697 | 6.11 |
Hongra Stream. | 0.36 | 105 | 302 | 2.65 |
Buyuk Stream | 1.56 | 105 | 1.310 | 11.48 |
Vanazit Stream. | 2.56 | 70 | 1.434 | 12.56 |
Zekere Stream | 1.85 | 105 | 1.554 | 13.61 |
Kizilev Stream | 6.34 | 105 | 5.326 | 46.65 |
Koyunham Stream | 1.47 | 105 | 1.235 | 10.82 |
Baltama Stream | 1.76 | 105 | 1.478 | 12.95 |
Tokmadin Stream | 1.28 | 245 | 2.509 | 21.98 |
Bal Stream | 0.67 | 155 | 831 | 7.28 |
Catalcam Stream | 0.89 | 125 | 890 | 7.80 |
Kirikli Stream | 0.80 | 95 | 6.08 | 5.33 |
Yagli Stream | 13.01 | 55 | 5.724 | 50.15 |
Total | 89.798 | 786.61 |
Hydroelectric power and hydroelectric values for selected streams in the Eastern Black Sea Basin (some data are from [17]).
In this study shows that Turkey has abundant hydropower energy sources and great renewable energy potential and is keen to reduce its dependence on fossil fuels by increasing its use of renewable energy resources. The hydropower sector is a good example of the increasing interest in generation of electricity with renewable resources. It is expected that the Government will call for tenders for hydropower licenses.
It is well known that the environmental impacts of hydro plants are minimal compared with alternative resources. They make use of our renewable “green energy” resource, without causing pollution. It is not enough for governments to support the development of renewable energy technologies. They must also support their commercial application in the country.
On the other hand, hydroelectric facilities are very valuable resources in Sakarya and Eastern Black Sea Basins, which has a great potential due to meteorological, topographic, hydraulics and hydrological conditions. Using this potential, the economic situation of people most of whom are unemployed and poor and live rural areas will be significantly improved.
Since small hydropower plants are river type hydropower plants, small hydropower potential should be used in the country especially in the Sakarya and Eastern Black Sea Basins. Because river type power plants are superior than the other plants, this type plants have not only cost and operational features but also good environmental effects.
energy value (kgm) power (kgm/s) specific weight (kg/m3) water discharge (m3/s) head of water drop (m) efficiency factor of turbine (%) statistic variance the number of ties of length (m). the statistic S is then standardized difference between ranks number of data rank value Sen’s slope denotes the variable indices median Sk terra watt hour giga watt hour mega watt hour kilo watt
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His main focus now is to unravel the mechanism of drought and heat stress response in plants to tackle climate change related threats in agriculture.",institutionString:null,institution:{name:"Indian Council of Agricultural Research",country:{name:"India"}}},{id:"4782",title:"Prof.",name:"Bishnu",middleName:"P",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/4782/images/system/4782.jpg",biography:"Bishnu P. Pal is Professor of Physics at Mahindra École\nCentrale Hyderabad India since July 1st 2014 after retirement\nas Professor of Physics from IIT Delhi; Ph.D.’1975 from IIT\nDelhi; Fellow of OSA and SPIE; Senior Member IEEE;\nHonorary Foreign Member Royal Norwegian Society for\nScience and Arts; Member OSA Board of Directors (2009-\n11); Distinguished Lecturer IEEE Photonics Society (2005-\n07).",institutionString:null,institution:{name:"Indian Institute of Technology Delhi",country:{name:"India"}}},{id:"69653",title:"Dr.",name:"Chusak",middleName:null,surname:"Limsakul",slug:"chusak-limsakul",fullName:"Chusak Limsakul",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Prince of Songkla University",country:{name:"Thailand"}}},{id:"75563",title:"Dr.",name:"Farzana Khan",middleName:null,surname:"Perveen",slug:"farzana-khan-perveen",fullName:"Farzana Khan Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75563/images/system/75563.png",biography:"Dr Farzana Khan Perveen (FLS; Gold-Medallist) obtained her BSc (Hons) and MSc (Zoology: Entomology) from the University of Karachi, MAS (Monbush-Scholar; Agriculture: Agronomy) and from the Nagoya University, Japan, and PhD (Research and Course-works from the Nagoya University; Toxicology) degree from the University of Karachi. 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