Host‐guest inclusion complex (IC) of vitamin C with β‐cyclodextrin (β‐CD) in aqueous medium has been explored by spectroscopic, physicochemical and calorimetric methods as stabilizer, carrier and regulatory releaser. Job plot has been drawn by UV‐visible spectroscopy to confirm the 1:1 stoichiometry of the host‐guest assembly. Stereo‐chemical nature of the inclusion complex has been explained by two‐dimensional (2D) NMR spectroscopy. Surface tension and conductivity studies further support the inclusion process. Association constants for the vitamin C‐β‐CD inclusion complex have been calculated by UV‐visible spectroscopy using both Benesi‐Hildebrand method and non‐linear programme, while the thermodynamic parameters have been estimated with the help of van’t Hoff equation. Isothermal titration calorimetric study has been performed to determine the stoichiometry, association constant and thermodynamic parameters with high accuracy.
Part of the book: Vitamin C