Interfacial electron transfer kinetics of the haem (FeIII/FeII) group in human hemoglobin molecules were investigated on glass/tin-doped indium oxide electrodes. Factors such as surface roughness, crystallinity, hydrophilicity and partial polarization of the working electrode played an important role to provide a more compatible microenvironment for protein adsorption. Results suggested that direct electron transfer from electrode to haem (FeIII)-H2O intermediate is coupled to proton at near physiological pH (I = 0.035, pH = 7.2).
Part of the book: Applications of the Voltammetry