The aim of this study was to analyze the pesticide residues in chili samples, collected from farmer’s field. Ultra performance liquid chromatography (UPLC) with BEH C18 column was used for this analysis work. A cheap and fast method for the simultaneous quantification of 12 residue of pesticides in chili has been developed. Samples were prepared according to Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) method and quantification was performed by using tunable ultra violet (TUV) detector. The method was applied for the analysis of the chili samples and results showed that most of the samples have detectable pesticide residues. The residues of acetamiprid and thiodicarb were detected only in three samples, whereas flubendiamide and mancozeb were detected in six samples and arbosulfan and Spinosad were detected in two and five samples, respectively. Out of the 30 chili samples, only 11 samples were found to be contaminated with pesticide residues with more than maximum residue limits (MRLs).
GC-MS/MS method has been developed and validated for the determination and quantification of 35 multi-class pesticide residues in grape samples. Pesticides are selected from different families including organochlorines, organophosphorus, carbamates, pyrethroids, triazines, triazoles, pyrazoles, etc. The QuEChERS-dSPE (dispersive solid-phase extraction) method was used for the extraction of residues of pesticide. An extra cleanup step was included with the help of a primary secondary amine (PSA) and graphitized carbon black (GCB). Recoveries ranged from 70 to 100% with 14% relative standard deviation (RSD). Other parameters such as precision, recoveries, limit of detection (LOD), limit of quantification (LOQ), and linearity were also studied. Finally, the proposed analytical method was successfully employed for the determination of residues of pesticide in grape samples.
Part of the book: Gas Chromatography