Adem Bardak

By Yuksel Bolek, Khezir Hayat, Adem Bardak and Muhammad Tehseen Azhar

Molecular characterization provides comprehensive information about the extent of genetic diversity, it assists for the development of an effective, highly accurate, and rapid marker‐assisted cotton breeding program. Due to one of the world’s leading fiber crops, molecular studies of cotton are being explored widely by cotton researchers. Cotton provides raw material to the textile industry among other products. Limitations in conventional breeding program for genetic improvement are due to the complexity and limited knowledge on economically important traits. The use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in molecular genetics. In the present scenerio, cotton molecular breeding has become a reliable source through the study and exploitation of its genetic diversity and due to better understanding of the cotton genomes using the next‐generation sequencing technologies. Cotton breeders should utilize genomics in breeding programs for effective selection of best parents for agronomic and fiber‐related traits, as well as for the development of resistance against biotic and abiotic stresses. The genomic research work could be based upon genotyping using DNA markers, quantitative trait loci mapping, genome‐wide associations, and next‐generation sequencing. The objective of this chapter is to describe evolution as well as utilization of various molecular markers and review the contribution of marker‐assisted selection (MAS) in cotton breeding.

Part of the book: Cotton Research

By Adem Bardak, Khezir Hayat, Oktay Erdogan, Zahid Mahmood, Noor-ul-Islam Khan, Muhammad Atif Iqbal, Halil Tekerek, Mehboob-ur-Rahman and Hafiz Muhammad Imran

The genus Gossypium provides natural fiber for textile industry worldwide. Genetic improvement in cotton for traits of interest is not up to mark due to scarcity of adequate information about fiber production and quality. Use of DNA markers for overcoming the issues of selection associated with complex traits is the ultimate choice which may lead to initiate breeding by design. Numerous marker-trait associations have been identified for economical traits using linkage analysis in cotton. Currently there is need for developing high-density genetic maps using next-generation sequencing approaches together with genome-wide association studies (GWAS). Efforts have been started in this direction and several QTLs including fiber quality, yield traits, plant architecture, stomatal conductance and verticillium wilt resistance were identified. This chapter narrates genetic diversity, QTL mapping, association mapping and QTLs related to fiber quality traits. The incorporation of various genomic approaches and previously described marker strategies will pave the way for increase in fiber production.

Part of the book: Past, Present and Future Trends in Cotton Breeding

By Khezir Hayat, Adem Bardak, Mehboob-ur-Rahman, Hafiz Muhammad Imran, Furqan Ahmad, Donay Parlak, Muhammad Azam, Muhammad Usmaan, Muhammad Adnan, Sidra Anjum and Rao Sohail Ahmad Khan

Improved fiber yield is considered a constant goal of upland cotton (Gossypium hirsutum) breeding worldwide, but the understanding of the genetic basis controlling yield-related traits remains limited. Dissecting the genetic architecture of complex traits is an ongoing challenge for geneticists. Two complementary approaches for genetic mapping, linkage mapping and association mapping have led to successful dissection of complex traits in many crop species. Both of these methods detect quantitative trait loci (QTL) by identifying marker–trait associations, and the only fundamental difference between them is that between mapping populations, which directly determine mapping resolution and power. Nowadays, the availability of genomic tools and resources is leading to a new revolution of plant breeding, as they facilitate the study of the genotype and its relationship with the phenotype, in particular for complex traits. Next Generation Sequencing (NGS) technologies are allowing the mass sequencing of genomes and transcriptomes, which is producing a vast array of genomic information with the development of high-throughput genotyping, phenotyping will be a major challenge for genetic mapping studies. We believe that high-quality phenotyping and appropriate experimental design coupled with new statistical models will accelerate progress in dissecting the genetic architecture of complex traits.

Part of the book: Plant Breeding