Simple sequence repeats (SSR) have been applied as useful markers for understanding cotton genetics. In the last decade, chromosome-deficient stocks of Gossypium hirsutum L. were used in the development of chromosome substitution lines for G. barbadense L., G. tomentosum Nuttall et Seemann, and G. mustelinum Watt chromosomes or chromosome segments. Several DNA markers have already been assigned to the individual chromosomes of G. hirsutum. We created new cotton monosomic lines in Uzbekistan after irradiation of seeds by thermal neutrons or pollen gamma‐irradiation to complement other global efforts in the development of cotton chromosome substitution lines. The primary objective of this chapter is to report the use of chromosome-specific SSR markers and a well-defined tester set of cotton translocation lines from the Cotton Cytogenetic Collection at Texas A&M AgriLife Research to confirm chromosome specificity of monosomic lines in Uzbekistan cytogenetic collection of cotton. Our results have assigned several different monosomic lines to the chromosomes 2, 4, 6, and telosome 11 At‐subgenome and chromosomes 18 and 20 or 22 Dt‐subgenome. These lines will be very useful in molecular mapping, the creation of substitution lines, and cotton breeding.
Part of the book: Cotton Research
Cotton is one of the most important crops in the world. The Gossypium genus is represented by 50 species, divided into two levels of ploidy: diploid (2n = 26) and tetraploid (2n = 52). This diversity of Gossypium species provides an ideal model for studying the evolution and domestication of polyploids. In this regard, studies of the origin and evolution of polyploid cotton species are crucial for understanding the ways and mechanisms of gene and genome evolution. In addition, studies of polyploidization of the cotton genome will allow to more accurately determine the localization of QTLs that determine fiber quality. In addition, due to the fact that cotton fibers are single trichomes originating from epidermal cells, they are one of the most favorable model systems for studying the molecular mechanisms of regulation of cell and cell wall elongation, as well as cellulose biosynthesis.
Part of the book: Model Organisms in Plant Genetics