Chapters authored
Structure, Oligomerisation and Interactions of β-Lactoglobulin
β-Lactoglobulin (βLg), as the most abundant whey protein in ruminant milk and as a useful model protein, is the subject of countless biophysical studies in the literature, yet its physiological role is hitherto unknown. This chapter deals with studies that focus on the structure of βLg, its oligomeric behaviour and the interactions that this protein participates in. These and further studies are necessary to understand how the protein’s physicochemical properties may influence the processing, digestion and immunogenicity of ruminant milks and their products. However, there is also a need for research into the interactions that occur naturally between βLg and other components in milk, as this may give us insight into the physiological role of the protein.
Part of the book: Milk Proteins
Microfluidics for Small-Angle X-ray Scattering
Small-angle X-ray scattering is a well-established biophysical technique, whilst micro-fluidics is proving to be a convenient technology for creating miniaturised multifunctional devices. Both fields are highly versatile and find use in multiple scientific disciplines. Together, they offer the potential to obtain structural information on biomacromolecules, nanoparticles and condensed matter, in a high-throughput manner and with enhanced time-resolution capabilities. This chapter provides practical design considerations for X-ray-based microfluidic systems and examines some of the existing microfluidic platforms used in conjunction with small-angle X-ray scattering. As the exclusive advantages of microfluidics become recognised and accessible, the prevalence of microfluidic sample environments in X-ray scattering measurements will hopefully increase.
Part of the book: X-ray Scattering